Method for cultivating green algae by recycling laver cultivation net curtain

Abstract

The invention discloses a method for cultivating green algae by recycling a laver cultivation net curtain, which comprises the following steps: firstly, cultivating laver, and cleaning the cultivation net curtain after the laver cultivation period is ended; then, enteromorpha spp attachment and cultivation nursing are carried out, and when the concentration of enteromorpha spores used for spp attachment is 104-105 / ml, the yield of enteromorpha reaches the highest; by means of the culture method, the optimal enteromorpha attached spore concentration can be obtained, the highest yield is obtained, and meanwhile the utilization rate of a sea area and culture equipment is remarkably increased.

Description

Technical Field

[0001] The present invention relates to a method for culturing green algae, in particular to a method for culturing green algae by recycling laver cultivation net curtains, and belongs to the technical field of bioengineering. Background Art

[0002] Laver is one of the main cultivated algae in China and has important economic value. The cultivation season of Zhejiang hairtail laver ( Porphyra haitanensis ) is from September of each year to January of the next year, and at most until February. After that, due to the too high water temperature, laver cannot be cultivated anymore. Thereafter, the laver net curtains will be taken out of the sea, cleaned and repaired, and then used again in September. Therefore, in the seasons with higher temperatures, there is no algae to cultivate in the sea area, which is not only a waste of sea area resources, but also not conducive to the stability of the sea area ecological environment. In addition, the poles for laver cultivation are idle in the sea area, causing waste. At the same time, the net curtains are placed on the shore for 6 - 7 months, and are easily weathered by long-term exposure to wind and rain, and the loss rate is very fast, resulting in unnecessary economic losses.

[0003] Enteromorpha prolifera ( Ulva prolifera ) grows fast and has strong stress resistance, and can grow in the environment of high temperature in summer. Young Enteromorpha prolifera can be used as a kind of marine flavor food, and there are certain reports on its cultivation in Japan. However, the large-scale outbreak of Enteromorpha prolifera in China has become an ecological disaster. One of the reasons for this phenomenon is that Enteromorpha prolifera in the far sea area cannot be salvaged, and the Enteromorpha prolifera that drifts to the near-shore beach has aged, with too high cellulose and the protein has been exhausted, so it cannot be eaten. And there is a history and technology of cultivating Enteromorpha prolifera in Ningbo and its surrounding sea areas, and Enteromorpha prolifera in Ningbo has been used as a delicious food in Ningbo and its surrounding coastal cities for a long time. Enteromorpha prolifera thousand-layer cakes, Enteromorpha prolifera peanuts, and Enteromorpha prolifera twist bread are well-known. In addition, Enteromorpha prolifera can be exported to earn foreign exchange and is in short supply. At present, the export volume only accounts for 50% of the quota that can be exported to Japan, and it has great cultivation potential. The main bottleneck restricting the development of the Enteromorpha prolifera industry at present is the insufficient output of fresh wild Enteromorpha prolifera, so how to increase the output of Enteromorpha prolifera is an important industrial problem.

[0004] The laver cultivation net curtains are idle for a long time, and there is a certain empty period in the sea area for seaweeds at present. How to effectively utilize this empty period in the sea area and the idle net curtains to cultivate green algae Enteromorpha prolifera, there is no relevant technical report at present. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a method for culturing green algae by recycling laver cultivation net curtains in view of the deficiencies in the prior art. On the basis of previous research, the present invention has developed a method for further cultivating green algae Enteromorpha prolifera by using laver cultivation net curtains ( Ulva prolifera)Aquaculture and related supporting technologies make full use of the empty period in the sea area and idle net curtains, while promoting the accumulation of blue carbon in the sea area. Taking Enteromorpha prolifera as an example, other green algae, including Ulva lactuca, Monostroma nitidum and other green algae, can be modified under the inspiration of the present invention to achieve the purpose of aquaculture.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A method for recycling laver culture net curtains for green algae culture, the method for using laver culture net curtains for green algae culture includes: during the intermittent period of laver culture, using laver culture net curtains for the culture of large green algae, and establishing a relevant technical system.

[0007] In the above technical solution, the method for using laver culture net curtains for green algae culture further includes: after the cultivation of Pyropia haitanensis ends, using the idle laver culture net curtains to cultivate Enteromorpha prolifera, and performing aquaculture maintenance, harvesting and processing.

[0008] In the above technical solution, the method for using laver culture net curtains for green algae culture specifically includes the following steps: (1) Conduct the cultivation of Pyropia haitanensis: Drive piles in the sea area, build a cultivation raft frame, conduct laver seedling cultivation, attach laver sporelings to the net curtain, and place the net curtain on the cultivation raft frame in the sea area during the season suitable for laver cultivation for cultivation; (2) Cleaning of the culture net curtain: Recover the net curtain idle after cultivating laver in step (1) in the season of recovering the laver culture net curtain, clean the net curtain to wash off the attachments, and disinfect it for later use; (3) Attachment of Enteromorpha prolifera seedlings and aquaculture care: Immerse the disinfected net curtain in step (2) in the artificially prepared Enteromorpha prolifera spore liquid for seedling attachment, set the Enteromorpha prolifera spore concentration to 10 3 ~10 5 , and then put the Enteromorpha prolifera net curtain into the sea and conduct aquaculture care; (4) Harvesting and processing of Enteromorpha prolifera: Harvest and process Enteromorpha prolifera after it reaches the harvesting specification.

[0009] In the above technical solution, in step (1), the season suitable for laver cultivation is from September of each year to January of the following year.

[0010] In the above technical solution, in step (2), the cleaning of the culture net curtain specifically includes: Recover the culture net curtain at the end of January every year, first wash it with a brush to wash off the laver attachments on the net curtain, then soak the culture net curtain in hydrochloric acid for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, soak it in seawater for one day and then dry it for later use.

[0011] In the above technical solution, the hydrochloric acid has a mass concentration of 0.1%.

[0012] In the above technical solution, in step (3), the Enteromorpha is the Enteromorpha prolifera algal strain wild in Ningbo ( Ulva prolifera ).

[0013] In the above technical solution, in step (3), for the artificially prepared Enteromorpha prolifera spore liquid, artificial shearing stimulation is used to promote the release of Enteromorpha prolifera spores; the concentration of the Enteromorpha prolifera spores is 10 3 , 10 4 , 10 5 cells / mL, preferably 10 4 cells / mL or 10 5 cells / mL.

[0014] In the above technical solution, in step (3), for the cultivation and care of Enteromorpha prolifera, it mainly involves cleaning the miscellaneous algae on the Enteromorpha prolifera raft to prevent the attachment of miscellaneous algae. The miscellaneous algae mainly include Grateloupia filicina ( Grateloupia filicina ) and Sargassum horneri( Sargassum horneri ).

[0015] In the above technical solution, in step (4), the harvesting and processing of Enteromorpha prolifera include: according to different water temperatures and the growth rate of Enteromorpha prolifera, harvesting is carried out when Enteromorpha prolifera grows to 40 - 50 cm; during harvesting, a laver harvesting boat is used to harvest the cultivated Enteromorpha prolifera in turn; the processing of the cultivated Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing impurities, and packaging of Enteromorpha prolifera.

[0016] Combined with the above technical solution and the solved technical problems, the advantages and positive effects of the technical solution to be protected by the present invention are analyzed from the following aspects: The present invention has simple processes and is easy to operate, without the need for complex equipment or cumbersome steps, making the entire production process more efficient and controllable. The present invention is an expansion based on the original laver cultivation facilities, extending the sea area cultivation time, increasing the cultivation yield per unit sea area, greatly improving the utilization rate of the unit sea area, and increasing the economic output of the unit sea area. In addition, the cultivation process can effectively absorb nitrogen and phosphorus elements and promote biological carbon fixation. Since the nutrients absorbed during the sea area cultivation of Pyropia haitanensis and Enteromorpha prolifera are different, it can promote the nitrogen and phosphorus nutrient balance in the sea area, increase the unit yield, similar to the rotation cultivation on land, comprehensively utilize the nutrients in the sea area, and improve the cultivation yield and recovery rate.

[0017] The expected benefits and commercial value after the transformation of the technical solution of the present invention are: providing a new technical approach for the industrial production of Enteromorpha prolifera; improving the utilization rate of the sea area and increasing economic benefits. Description of the Drawings

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the attached drawings required for use in the embodiments of the present invention. Obviously, the attached drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other attached drawings can be obtained based on these attached drawings without creative efforts.

[0019] Figure 1 It is a schematic diagram of the rotational cultivation of laver and Enteromorpha prolifera in different seasons in the sea area; Figure 2 It is a schematic diagram of the structure of the cultivation net curtain for laver and Enteromorpha prolifera (where 1 is the mooring cable, 2 is the raft frame, and 3 is the net curtain); Figure 3 It is a scene diagram of the large-scale cultivation of Enteromorpha prolifera in the sea area in Example 3; Figure 4 It is a scene diagram of the large-scale cultivation of Enteromorpha prolifera in the sea area in Example 4. Specific embodiments

[0020] The following describes in detail the specific embodiments of the technical solutions of the present invention, but the present invention is not limited to the following description: As Figure 1 shown, the cultivation period of laver is from September of each year to January of the following year. Due to factors such as weather, temperature, and light, the main cultivation time in a year is 5 months. During the period when the sea area temperature is relatively high, laver cannot be cultivated, and the laver cultivation net curtain and the sea area are in an idle state. Enteromorpha prolifera has stronger adaptability. Wild Enteromorpha prolifera in the natural sea area exists from October to March, and Enteromorpha prolifera begins to naturally recede in February - March, and fresh Enteromorpha prolifera cannot be obtained. Therefore, artificial cultivation can be carried out during February to June of each year, which can greatly utilize the idle sea area and equipment. Figure 2 It is a schematic diagram of the net curtain used for laver cultivation, where: 1 is the mooring cable, 2 is the raft frame, and 3 is the net curtain. The cultivation conditions of laver and Enteromorpha prolifera are similar, and the same cultivation raft frame and harvesting equipment can be shared.

[0021] The technical method for cultivating green algae using the laver cultivation net curtain provided by the present invention includes the following steps: (1) Conduct laver cultivation: Drive piles in the sea area, build a cultivation raft frame, conduct laver seedling cultivation, attach laver spore seedlings to the net curtain, and place the net curtain on the cultivation raft frame in the sea area for cultivation from September of each year to January of the following year.

[0022] (2) Clean the cultivation net curtain: Recover the cultivation net curtain in February of each year. First, wash it once with a brush to wash off the laver attachments on the net curtain, then soak the cultivation net curtain in hydrochloric acid with a concentration of 0.1% for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, soak it in seawater for one day, and then dry it for standby.

[0023] (3)Enteromorpha prolifera seeding and aquaculture care: Soak the net curtain in the artificially prepared spore solution of wild Enteromorpha prolifera in Ningbo for seeding. The spore solution of Enteromorpha prolifera is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set to 0, 10 3 , 10 4 , 10 5 cells per milliliter. After the net curtain is seeded, the net curtain is put into the sea and aquaculture care is carried out, mainly to prevent the attachment of miscellaneous algae.

[0024] (4)Enteromorpha prolifera harvesting and processing: According to different water temperatures and the growth rate of Enteromorpha prolifera, when Enteromorpha prolifera grows to 40 - 50 cm long, it can be harvested. The harvesting uses a laver harvesting boat to take turns harvesting and weighing the cultured Enteromorpha prolifera. The processing of cultured Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing sundries, and packaging of Enteromorpha prolifera.

[0025] The applicant found that Enteromorpha prolifera spores with different concentrations have a significant impact on the growth and yield of Enteromorpha prolifera. The embodiments of the present invention studied the growth of Enteromorpha prolifera under different concentrations of Enteromorpha prolifera spores, and achieved some positive effects during the research and development or use process, and indeed have great advantages compared with the prior art. The following content is described in combination with the data, charts, etc. of the test process. Example 1:

[0026] A method for culturing green algae by recycling laver culture net curtains. In the embodiment of the present invention, when the concentration of Enteromorpha prolifera spores provided is 0 cells per milliliter (i.e., natural seeding in the sea area), the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1)Carry out laver culture: Drive piles in the sea area, build a culture raft frame, carry out laver seedling cultivation, attach laver spore seedlings to the net curtain, and place the net curtain on the culture raft frame in the sea area for cultivation from September of each year to January of the next year.

[0027] (2)Clean the culture net curtain: Recover the culture net curtain in February of each year. First, wash it once with a brush to wash off the laver attachments on the net curtain, then soak the culture net curtain in 0.1% hydrochloric acid for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, soak it in seawater for one day and then dry it for standby.

[0028] (3)Enteromorpha prolifera seeding and aquaculture care: Soak the net curtain in the artificially prepared spore solution of wild Enteromorpha prolifera in Ningbo for seeding. The spore solution of Enteromorpha prolifera is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set to 0 cells per milliliter. After the net curtain is seeded, the net curtain is put into the sea and aquaculture care is carried out, mainly to prevent the attachment of miscellaneous algae.

[0029] (4)Enteromorpha prolifera harvesting and processing: According to different water temperatures and the growth rate of Enteromorpha prolifera, when it grows to 40 - 50 cm long, it can be harvested. The harvesting uses a laver harvesting boat to take turns harvesting and weighing the cultured Enteromorpha prolifera. The results show that when Enteromorpha prolifera naturally attaches seedlings in the sea area without artificial seeding, it takes about 90 days from setting the net to the first harvest, with a single - yield of 1300 catties per mu. After that, it is harvested once every about 60 days (the results are shown in Table 1). Compared with the seeding group, the first - harvest time is significantly extended. The processing of cultured Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing impurities, and packaging. Example 2:

[0030] A method for culturing green algae by recycling laver cultivation net curtains. In this embodiment of the present invention, when the concentration of Enteromorpha prolifera spores provided is 10 3 cells per milliliter, the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1)Carry out laver cultivation: The same as in Example 1.

[0031] (2)Clean the cultivation net curtain: The same as in Example 1.

[0032] (3)Enteromorpha prolifera seeding and cultivation care: Immerse the net curtain in the artificially prepared Enteromorpha prolifera spore liquid of Ningbo wild Enteromorpha prolifera for seeding. The Enteromorpha prolifera spore liquid is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set to 10 3 cells per milliliter. After the net curtain is seeded, it is put into the sea and cultivated, mainly to prevent the attachment of miscellaneous algae.

[0033] (4)Enteromorpha prolifera harvesting and processing: According to different water temperatures and the growth rate of Enteromorpha prolifera, when it grows to 40 - 50 cm long, it can be harvested. The harvesting uses a laver harvesting boat to take turns harvesting and weighing the cultured Enteromorpha prolifera. The first - harvest time is 45 days, saving 45 days compared with the non - seeding group. After that, it is harvested once every 35 days, with a single - yield of 1400 catties per mu (the results are shown in Table 1). The processing of cultured Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing impurities, and packaging. Example 3:

[0034] A method for culturing green algae by recycling laver cultivation net curtains. In this embodiment of the present invention, when the concentration of Enteromorpha prolifera spores provided is 10 4 cells per milliliter, the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1)Carry out laver cultivation: The same as in Example 1.

[0035] (2)Clean the cultivation net curtain: The same as in Example 1.

[0036] (3) Enteromorpha prolifera seeding and aquaculture care: Soak the net curtain in the artificially prepared spore liquid of wild Enteromorpha prolifera in Ningbo for seeding. The Enteromorpha prolifera spore liquid is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set at 10 4 cells / ml. After the net curtain is seeded, lower the net curtain into the sea and carry out aquaculture care, mainly to prevent the attachment of miscellaneous algae.

[0037] (4) Enteromorpha prolifera harvesting and processing: The scene map of large-scale aquaculture of Enteromorpha prolifera in the sea area is as shown in Figure 3 . The harvesting and processing of Enteromorpha prolifera are the same as those in Example 1. The final result shows that the first harvesting time is 30 days, which saves 60 days compared with the non-seeding group. After that, it is harvested once every 30 days, and the single yield is 1500 catties / mu (the results are shown in Table 1). Example 4:

[0038] A method for culturing green algae by recycling laver aquaculture net curtains. When the concentration of Enteromorpha prolifera spores provided in the embodiment of the present invention is 10 5 cells / ml, detect and analyze the harvesting cycle and yield of Enteromorpha prolifera. The specific steps are as follows: (1) Carry out laver aquaculture: The same as in Example 1.

[0039] (2) Cleaning of aquaculture net curtains: The same as in Example 1.

[0040] (3) Enteromorpha prolifera seeding and aquaculture care: Soak the net curtain in the artificially prepared spore liquid of wild Enteromorpha prolifera in Ningbo for seeding. The Enteromorpha prolifera spore liquid is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set at 10 5 cells / ml. After the net curtain is seeded, lower the net curtain into the sea and carry out aquaculture care, mainly to prevent the attachment of miscellaneous algae.

[0041] (4) Enteromorpha prolifera harvesting and processing: The scene map of large-scale aquaculture of Enteromorpha prolifera in the sea area is as shown in Figure 4 . The harvesting and processing of Enteromorpha prolifera are the same as those in Example 1. The final result shows that the first harvesting time is 30 days, which saves 60 days compared with the non-seeding group. After that, it is harvested once every 30 days, and the single yield is 1500 catties / mu (the results are shown in Table 1).

[0042] Table 1 shows the effects of different Enteromorpha prolifera spore concentrations on the final yield in different examples Sporulation inoculation concentration First harvest time Second harvest time Third harvest time Fourth harvest time Fifth harvest time Single yield Total yield (2.1 - 6.30) 0 90 days 60 days -- -- -- 1300 jin / mu 2600 jin / mu <![CDATA[10 3 CFU / mL]]> 45 days 35 days 35 days 35 days -- 1400 jin / mu 5200 jin / mu <![CDATA[10 4 CFU / mL]]> 30 days 30 days 30 days 30 days 30 days 1500 jin / mu 7500 jin / mu <![CDATA[10 5 CFU / mL]]> 30 days 30 days 30 days 30 days 30 days 1500 jin / mu 7500 jin / mu (Note: The highest aquaculture season of Enteromorpha prolifera is up to June 30th, calculated as 150 days (from February 1st to June 30th). Without spore seeding, 2 crops can be harvested. With low-density seeding, 4 crops can be cultured. With high-density seeding, 5 crops can be cultured and harvested. There are significant differences in the total output.)

[0043] As can be seen from Table 1, in the present invention, the net curtain is immersed in the artificially prepared spore liquid of wild Enteromorpha prolifera in Ningbo for seedling attachment, and different concentrations of Enteromorpha prolifera spore concentrations are screened. The highest yield is obtained under the condition of a concentration of 10 4 -10 5 cells per milliliter.

[0044] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention shall be covered by the protection scope of the present invention.

Claims

1. A method for cultivating green algae by recycling laver cultivation net curtains, characterized in that, The method for culturing green algae using laver cultivation net curtains includes: during the intermittent period of laver cultivation, using laver cultivation net curtains to culture large green algae, and establishing a relevant technical system.

2. The method according to claim 1, wherein The method for culturing green algae using laver cultivation net curtains further includes: after the cultivation of Gracilaria lemaneiformis is completed, using the idle laver cultivation net curtains to culture Enteromorpha prolifera, and performing cultivation maintenance, harvesting and processing.

3. The method according to claim 2, characterized in that The method for culturing green algae using laver cultivation net curtains specifically includes the following steps: (1) Conduct Gracilaria lemaneiformis cultivation: Drive piles in the sea area, build a cultivation raft frame, conduct laver seedling cultivation, attach laver sporelings to the net curtains, and place the net curtains on the cultivation raft frame in the sea area during the season suitable for laver cultivation for cultivation; (2) Cleaning of cultivation net curtains: Recover the net curtains idle after laver cultivation in step (1) in the season when laver cultivation net curtains are recovered, clean the net curtains to wash off the attachments, and disinfect them for later use; (3)Ulva prolifera spore attachment and aquaculture care: Soak the disinfected net curtain in step (2) in the artificially prepared Ulva prolifera spore liquid for spore attachment, set the Ulva prolifera spore concentration to 10 3 ~10 5 cells / ml, and then lower the Ulva prolifera net curtain into the sea and conduct aquaculture care; (4) Harvesting and processing of Enteromorpha prolifera: Harvest and process Enteromorpha prolifera after it reaches the harvesting specification.

4. The method according to claim 3, wherein In step (1), the season suitable for laver cultivation is from September of each year to January of the next year.

5. The method according to claim 3, wherein In step (2), the cleaning of the cultivation net curtains specifically includes: Recover the cultivation net curtains at the end of January each year. First, wash them once with a brush to wash off the laver attachments on the net curtains, then soak the cultivation net curtains in hydrochloric acid for 1 hour, rinse them with tap water to remove the excess hydrochloric acid residue, soak them in seawater for one day and then dry them for later use.

6. The method according to claim 5, characterized in that, The hydrochloric acid has a mass concentration of 0.1%.

7. The method according to claim 3, characterized in that, In step (3), the Enteromorpha prolifera is the Enteromorpha prolifera algal species wild in Ningbo ( Ulva prolifera ).

8. The method according to claim 3, characterized in that In step (3), for the artificially prepared Enteromorpha prolifera spore liquid, artificial shearing stimulation is used to promote the release of Enteromorpha prolifera spores; the concentration of the Enteromorpha prolifera spores is 10 3 , 10 4 , 10 5 spores per milliliter.

9. The method according to claim 3, wherein In step (3), the cultivation and care of Enteromorpha mainly involve the cleaning of miscellaneous algae on the Enteromorpha raft to prevent the attachment of miscellaneous algae. The miscellaneous algae mainly include Grateloupia filicina ( Grateloupia filicina ) and Sargassum horneri ( Sargassum horneri ).

10. The method according to claim 3, characterized in that, In step (4), the harvesting and processing of Enteromorpha prolifera include: According to different water temperatures and the growth rate of Enteromorpha prolifera, harvest Enteromorpha prolifera when it grows to 40 - 50 cm; When harvesting, use a laver harvesting boat to harvest the cultivated Enteromorpha prolifera in turns; The processing of cultivated Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing sundries, and packaging of Enteromorpha prolifera.

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