Application of coronatine in preparation of accelerant for diffusion of porphyra yezoensis conchospores
By using 0.188-0.376 μmol/L of coronidin as a promoter during the release of slit norica shell spores, the problem of limited use of coronidin was solved, and a significant increase in the release of coronidin was achieved.
Patent Information
- Application Number
- CN202510552655.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-29
- Publication Date
- 2025-08-01
AI Technical Summary
The use of coronin is relatively limited and has not effectively promoted the release of spores of streaky nori shells.
The concentration of coronin at a concentration of 0.188-0.376 μmol/L is used as a promoter to promote the release of spores of streaky scalyptus.
Within this concentration range, coronidin significantly promotes the daily discharge of shell spores.
Smart Images
Figure CN120391438A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a use of coronatine. Background Art
[0002] Plant promoters can regulate the process of plant growth and development. Applying promoters is an important means to promote increased production and high-quality production, and is also an effective method to resist stress. Coronatine (COR) is one of the metabolites secreted by Pseudomonas syringae, and is a novel plant growth regulator. The molecular formula of COR is C 18 H 25 NO4, which is obtained by condensation of coronamic acid (CMA) and coronafacic acid (CFA) through an amide bond. At present, the use of coronatine is relatively limited. Summary of the Invention
[0003] The purpose of the present invention is to provide a new use of coronatine, which is an application of coronatine in the preparation of a promoter for the liberation of conchospores of Pyropia yezoensis.
[0004] The technical solution of the present invention is as follows:
[0005] An application of coronatine in the preparation of a promoter for the liberation of conchospores of Pyropia yezoensis, characterized in that: the concentration of the coronatine is 0.188 - 0.376 μmol / L.
[0006] The present invention promotes the daily liberation amount of conchospores by adding coronatine at a concentration of 0.188 - 0.376 μmol / L. Description of the Drawings
[0007] The present invention will be further described below with reference to the drawings and embodiments.
[0008] Figure 1 It is a schematic diagram of the daily average liberation amount of the shell filaments of Pyropia yezoensis under the treatment of coronatine at different concentrations. Detailed Embodiments
[0009] An application of coronatine in the preparation of a promoter for the liberation of conchospores of Pyropia yezoensis, wherein the concentration of the coronatine is 0.188 - 0.376 μmol / L.
[0010] Specific experiments:
[0011] 1 Materials and Methods
[0012] 1.1 Experimental materials
[0013] The tested free filaments of Pyropia yezoensis were taken from the Y-9101 strain of the national Pyropia germplasm bank.
[0014] 1.2 Experimental equipment
[0015] Light incubator MGZ-120B-3, Nikon-E800 biological microscope (Japan), Yuanfang SFIM-300 spectral scintillation illuminometer, food blender, WALZ-uKEA0388 chlorophyll fluorescence meter PAM (Germany), etc.
[0016] Table 1 Reagents
[0017]
[0018] 1.3 Experimental methods
[0019] (1) Inoculation and culture of filaments
[0020] On May 8, 2023, a food blender was used to break the free filaments of Pyropia yezoensis into small segments of 60-80 μm, which were inoculated onto the cleaned shells of Ostrea rivularis. The inoculation density was 300 inds / cm2. The culture conditions were set as follows: the light intensity was 20 μmol / (m2·s), the temperature in the incubator was 20 °C, and the light cycle was set as 12L:12D.
[0021] (2) Measurement of the amount of conchospore release
[0022] After 180 days of culture of the shell filaments, the experiment of coronatine treatment was carried out. The culture conditions were set as follows: the light intensity was 70 μmol / (m2·s), the temperature was (22±1) °C, and the temperature in the dark condition was (19±1) °C. The light cycle was set as 8L:16D. The concentrations of coronatine were set as 0, 0.019, 0.038, 0.188, 0.376 μmol / L. The coronatine treatment solution was added every 3 days until the end of the conchospore release experiment. It was continuously measured for 15 days. At 12:00 every day, a biological microscope was used to count the daily average amount of conchospore release. There were 7 shells in each group, and a total of 7 days were counted. The spore liquid of each shell was fixed in a 5 ml test tube, mixed evenly, and then counted in five fields of view in a counting frame, and then the amount of conchospore release of each shell was calculated. The calculation formula for the release amount is as follows:
[0023] N = V÷[R 2 ×π×D]×n
[0024] In the formula, N is the total amount of spores, V is the volume of the spore liquid, R is the radius of the field of view, π is the pi, D is the depth of the field of view, and n is the average number of spores in each field of view.
[0025] After counting, clean the surface of the shell and replace the culture seawater.
[0026] 2 Results
[0027] 2.1 Effects of coronatine treatment at different concentrations on the amount of conchospore release
[0028] Compared with the control, adding coronatine at a concentration of 0.019 - 0.038 μmol / L decreased the daily average liberation amount of conchospores, while adding coronatine at a concentration of 0.188 - 0.376 μmol / L promoted the daily liberation amount of conchospores. The results showed that adding coronatine at a concentration of 0.188 - 0.376 μmol / L could be used as a promoter for the liberation of conchospores of Pyropia yezoensis.
Claims
1. Application of coronatine in preparing a promoter for promoting the release of conchospores of Pyropia yezoensis, characterized in that: The concentration of the coronatine is 0.188 - 0.376 μmol / L.