Polysaccharide and oligopeptide composition and application thereof in promoting plant growth

Abstract

The invention relates to a polysaccharide and oligopeptide composition and application thereof in promoting plant growth. The polysaccharide and oligopeptide composition comprises the following components: xanthan gum, algal polysaccharides, a grifola frondosa extract, soybean oligopeptide, wheat oligopeptide, corn oligopeptide, modified silicon dioxide, itaconic acid, indolebutyric acid, glycine, glutamic acid, citric acid and fatty alcohol-polyoxyethylene ether. Wherein the modified silicon dioxide is obtained by reacting silicon dioxide with an acrylate-based silane coupling agent and then reacting with acrylic acid esterified humic acid. According to the plant growth promoter, the modified silicon dioxide is compounded with various substances such as polysaccharide and oligopeptide, the components are good in compatibility, the stability of the product is improved, the action time is prolonged, the absorption efficiency is improved, plant growth can be efficiently promoted, the defects of existing products are overcome, and the plant growth promoter has important significance on development of plant growth promoter products.

Description

Technical Field

[0001] The present invention relates to the field of fertilizers, and particularly to a polysaccharide and oligopeptide composition and its application in promoting plant growth. Background Art

[0002] Plant growth promoters can improve the cell division, differentiation and growth rate of plants, and also have a regulatory and promoting effect on the organ development of plants. They play an important role in agricultural production and can effectively improve the quality and yield of crops.

[0003] Current plant growth promoter products are usually composed of a single component and can only achieve a single function when used. For example, indoleacetic acid and naphthaleneacetic acid are used to promote the rooting of cuttings and prevent organ abscission; gibberellin is used to promote cell elongation, seed germination and increase yield; 6-benzyladenine is used to promote cell division and improve the growth rate of plants, flower bud differentiation and fruit development.

[0004] Polysaccharides and oligopeptides are also important substances with the ability to promote plant growth. Polysaccharides can not only increase the quantity and variety of microorganisms involved in nutrient transformation and release in the soil, thereby improving the absorption efficiency of plants for nutrients; but also form complexes with microorganisms in the soil, enhancing the adhesion and adsorption force of plant roots and promoting the speed and efficiency of plant nutrient absorption; at the same time, polysaccharides can also improve the soil structure, increase the porosity and water permeability in the soil, providing an ideal environment for plant development; polysaccharides themselves can also enhance the adhesion and fixation force of plant cells, promote the growth of roots and leaves, and improve the stress resistance of crops. Oligopeptides can increase the photosynthesis efficiency of plant leaves, efficiently synthesize more organic substances, so as to accelerate the growth of plants; they can also stimulate the growth of plant roots, promote the development and branching of roots, increase the absorption area and activity of roots, and thus absorb growth substances more fully; oligopeptides can also induce plants to produce defensive substances, such as antioxidant enzymes, etc., improve the antioxidant capacity and disease resistance of plants, and enhance the adaptability of plants to adversity.

[0005] However, in actual production, there are often multiple requirements, such as simultaneously increasing the plant growth rate, increasing the fertilizer absorption amount, improving the stress resistance of plants, etc. Single-component plant growth promoters need to be added and applied multiple times to meet the requirements, which is very inconvenient to use. There are also products that mix multiple components to form a composition, simplifying the usage process and meeting multiple requirements at the same time. However, such products also have problems such as poor compatibility between components, uneven mixing, and easy deterioration and inactivation of active components such as polysaccharides and oligopeptides, reducing the value of plant growth promoters and making it difficult to achieve ideal functions and effects.

[0006] In summary, it is urgent to develop a new technical solution to solve the problems existing in the prior art. Summary of the Invention

[0007] Based on this, the present invention provides a polysaccharide and oligopeptide composition and its application in promoting plant growth. The present invention uses modified silica in combination with various substances such as polysaccharides and oligopeptides. The components are well compatible with each other, which not only improves the stability of the product, but also increases the action time and absorption efficiency, and can efficiently promote plant growth, overcoming the deficiencies existing in existing products, and is of great significance to the development of plant growth promoter products.

[0008] An object of the present invention is to provide a polysaccharide and oligopeptide composition, and the polysaccharide and oligopeptide composition comprises the following components in parts by mass:

[0009]

[0010] Wherein,

[0011] The modified silica is obtained by reacting silica with an acrylate-based silane coupling agent and then reacting with acrylated humic acid.

[0012] Furthermore, the preparation method of the modified silica comprises the following steps:

[0013] S1. Blend silica and an acrylate-based silane coupling agent, and perform ultrasonic heating to obtain intermediate product 1;

[0014] S2. Blend humic acid, acryloyl chloride and a catalyst, and after reaction, obtain intermediate product 2;

[0015] S3. Blend the intermediate product 1, intermediate product 2 and an initiator, and perform ultrasonic heating to obtain modified silica.

[0016] Furthermore, in step S1, the temperature of the ultrasonic heating is 30 - 90 °C, and the time is 5 - 20 h.

[0017] Furthermore, in step S2, the temperature of the reaction is 0 - 30 °C, and the time is 12 - 36 h.

[0018] Furthermore, in step S2, the catalyst is a base catalyst.

[0019] Furthermore, in step S3, the temperature of the ultrasonic heating is 60 - 80 °C, and the time is 12 - 36 h.

[0020] Furthermore, the mass ratio of the intermediate product 1 to the intermediate product 2 is 1:(0.5 - 3).

[0021] Furthermore, the Grifola frondosa extract is purchased from Shaanxi Yuanbeibei Biotechnology Co., Ltd.

[0022] Another object of the present invention is to provide the use of the above-mentioned polysaccharide and oligopeptide composition in promoting plant growth.

[0023] The present invention has the following beneficial effects:

[0024] The polysaccharide and oligopeptide composition of the present invention contains modified silica. First, the silica is treated with an acrylate-based silane coupling agent KH570 to obtain intermediate product 1 with unsaturated double bonds introduced on the surface. Then, humic acid and acryloyl chloride are subjected to an acylation reaction to obtain intermediate product 2 with double bonds introduced. Next, intermediate product 1 and intermediate product 2 are blended and polymerized under the action of an initiator to obtain a silica carrier grafted with humic acid macromolecules on the surface. On the one hand, this component improves the compatibility of silica with active ingredients such as polysaccharides and oligopeptides, thereby enhancing the stability of the composition; on the other hand, the surface-modified silica has a larger specific surface area, which is beneficial for the adsorption of polysaccharides and oligopeptides. At the same time, humic acid has a large number of active groups such as carboxyl groups, hydroxyl groups, and phenolic hydroxyl groups, which further increases the active sites of the modified silica, can form intermolecular forces with components such as polysaccharides and oligopeptides, improves the adsorption and binding effects, reduces the possibility of deterioration and failure of the active ingredients, and realizes the function of lasting and long-term effectiveness.

[0025] In addition, the silica modified by humic acid also has good absorption capacity for nutrients such as nitrogen and phosphorus in the soil, improves the utilization rate of conventional fertilizers and soil nutrients, and further promotes the growth and development of plants. In summary, the polysaccharide and oligopeptide composition of the present invention solves the problems in the prior art and provides a new idea for the development of plant growth promoters. Specific Embodiments

[0026] In order to more clearly illustrate the technical solution of the present invention, the following examples are listed. The raw materials, reactions, and post-treatment means appearing in the examples are all common raw materials on the market and technical means well-known to those skilled in the art, unless otherwise specified.

[0027] The terms "preferred", "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects in certain cases. However, in the same or other cases, other embodiments may also be preferred. In addition, the description of one or more preferred embodiments does not imply that other embodiments are not available, nor is it intended to exclude other embodiments from the scope of the present invention.

[0028] It should be understood that, except in any operating instance or otherwise indicated, all numbers representing, for example, the amounts of ingredients used in the specification and claims are to be understood as being modified in all instances by the term "about". Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and appended claims are approximations that vary depending upon the desired properties sought to be obtained by the present invention.

[0029] In the embodiments of the present invention, xanthan gum was purchased from Tokyo Chemical Industry (Shanghai) Chemical Industry Development Co., Ltd., and seaweed polysaccharide was purchased from Shaanxi Yuanbeibei Biotechnology Co., Ltd.

[0030] In the embodiments of the present invention, Grifola frondosa extract was purchased from Shaanxi Yuanbeibei Biotechnology Co., Ltd.

[0031] In the embodiments of the present invention, soybean oligopeptide, wheat oligopeptide, and corn oligopeptide were purchased from Xi'an Green Peptide Biotechnology Co., Ltd.

[0032] The fatty alcohol polyoxyethylene ether in the embodiments of the present invention is AEO-9.

[0033] The preparation method of the modified silica in the embodiments of the present invention comprises the following steps:

[0034] S1. Add 6 g of silica to 80 ml of ethanol-aqueous solution (ethanol: water = 1:4, v / v), then add 20 g of KH570, and react by ultrasonic wave at 80 °C for 4 h. After filtration, washing, and drying, intermediate product 1 is obtained;

[0035] S2. At 0 °C, add 5 g of humic acid to 20 ml of anhydrous dichloromethane, then add 1 g of triethylamine and 1 g of acryloyl chloride, and raise the temperature to 30 °C and react for 24 h. After filtration, washing, and drying, intermediate product 2 is obtained;

[0036] S3. Add 5 g of the intermediate product 1, 2 g of the intermediate product 2, and 0.3 g of ammonium persulfate to 100 ml of water, and react by ultrasonic wave at 80 °C for 6 h under a nitrogen atmosphere. After filtration, washing, and drying, modified silica is obtained.

[0037] All "parts" in the embodiments of the present invention refer to parts by mass.

[0038] Example 1

[0039] A polysaccharide and oligopeptide composition, comprising the following ingredients in parts by mass:

[0040]

[0041] The preparation method of the polysaccharide and oligopeptide composition comprises the following steps:

[0042] Mix the components in accordance with the above parts by mass and stir evenly to obtain the product.

[0043] Example 2

[0044] A polysaccharide and oligopeptide composition, comprising the following components in parts by mass:

[0045]

[0046]

[0047] The preparation method of the polysaccharide and oligopeptide composition comprises the following steps:

[0048] According to the above parts by mass, blend each component and stir evenly to obtain a product.

[0049] Example 3

[0050] A polysaccharide and oligopeptide composition, comprising the following components in parts by mass:

[0051]

[0052] The preparation method of the polysaccharide and oligopeptide composition comprises the following steps:

[0053] According to the above parts by mass, blend each component and stir evenly to obtain a product.

[0054] Comparative Example 1

[0055] A composition, the difference between this comparative example and Example 1 is that: the preparation method of the modified silica is replaced by: physically blending the intermediate product 1 obtained in step S1 with humic acid in a mass ratio of 2:1, and stirring evenly to obtain the modified silica, and other components and preparation methods are the same as those in Example 1.

[0056] Comparative Example 2

[0057] A composition, the difference between this comparative example and Example 1 is that: the soy oligopeptide, wheat oligopeptide and corn oligopeptide are replaced by xanthan gum of equal mass, and other components and preparation methods are the same as those in Example 1.

[0058] Test Example

[0059] Perform performance tests on the compositions prepared in the examples and comparative examples.

[0060] Test method:

[0061] Soak lettuce seeds in warm water at 25°C for 6 hours. After taking them out, conduct germination at 20°C. Transplant the seedlings after the first true leaf grows. Set up 6 treatment groups. In each treatment group, plant 3 lettuce seedlings with good growth and consistent weight into pots. Add 10 kg of soil to each pot, and apply 4 g of conventional chemical fertilizer (3 g of urea and 1 g of potassium dihydrogen phosphate) to each group, then stir and mix evenly. One group does not add other components, and the other five groups respectively add the compositions prepared in Examples 1-3 and Comparative Examples 1-2 according to 5 wt% of the chemical fertilizer. After 30 days of planting, take out the lettuce and measure the weight (fresh weight of the above-ground part), and calculate the average value of lettuce in each group. During this period, the management of lettuce follows the agricultural timing operation, and the cultivation temperature is controlled at 25-30°C.

[0062] After storing the compositions prepared in the examples and comparative examples at room temperature for 30 days, retest according to the above method.

[0063] The test results are shown in Table 1.

[0064] Table 1 Test Results

[0065]

[0066] It can be concluded from Table 1 that the polysaccharide and oligopeptide compositions prepared in the examples of the present invention can effectively promote plant growth, and the weight gain is significantly higher than that of conventional chemical fertilizers. In Comparative Example 2, the oligopeptide component was replaced, and the weight gain effect was significantly lower than that of the examples. At the same time, the composition of the present invention has good stability. After long-term storage, it can still effectively improve the growth of plants. In Comparative Example 1, the modified silica was replaced with a mixture of coupling agent-modified silica and humic acid, the adsorption effect on the active ingredient decreased, and the stability also decreased, and finally the weight gain effect was not ideal. In summary, the polysaccharide and oligopeptide compositions of the present invention overcome the deficiencies of the prior art and have good application prospects.

[0067] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, it is intended to include all changes falling within the meaning and scope of the equivalent elements of the claims in the present invention.

[0068] In addition, it should be understood that although this specification is described according to the embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A polysaccharide and oligopeptide composition, characterized in that, The polysaccharide and oligopeptide composition comprises the following components in parts by mass: Wherein, The modified silica is obtained by reacting silica with an acrylate-based silane coupling agent and then reacting with acrylated humic acid.

2. The polysaccharide and oligopeptide composition according to claim 1, wherein The preparation method of the modified silica comprises the following steps: S1. Blend silica and an acrylate-based silane coupling agent, and perform ultrasonic heating to obtain intermediate product 1; S2. Blend humic acid, acryloyl chloride and a catalyst, and react to obtain intermediate product 2; S3. Blend the intermediate product 1, intermediate product 2 and an initiator, and perform ultrasonic heating to obtain modified silica.

3. The polysaccharide and oligopeptide composition according to claim 2, wherein In step S1, the temperature of the ultrasonic heating is 30 - 90 °C, and the time is 5 - 20 h.

4. The polysaccharide and oligopeptide composition according to claim 2, wherein In step S2, the temperature of the reaction is 0 - 30 °C, and the time is 12 - 36 h.

5. The polysaccharide and oligopeptide composition according to claim 2, characterized in that, In step S2, the catalyst is a base catalyst.

6. The polysaccharide and oligopeptide composition according to claim 2, wherein In step S3, the temperature of the ultrasonic heating is 60 - 80 °C, and the time is 12 - 36 h.

7. The polysaccharide and oligopeptide composition according to claim 2, wherein The mass ratio of the intermediate product 1 to the intermediate product 2 is 1:(0.5 - 3).

8. Use of the polysaccharide and oligopeptide composition according to any one of claims 1 - 8 in promoting plant growth.

Images