A SNP marker and KASP primer set for detecting the resistance of tobacco xanthomonas campestris race 0 and its application

By developing SNP markers and KASP primer sets on chromosome 22 of the tobacco genome, the problem of lack of effective markers in tobacco wildfire resistance breeding was solved, achieving efficient genotype screening and cost reduction in the breeding process.

CN120464766BActive Publication Date: 2026-06-26CHINA NAT TOBACCO CORP HEILONGJIANG CO MUDANJIANG TOBACCO SCIEN CE INSTIT
View PDF 4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA NAT TOBACCO CORP HEILONGJIANG CO MUDANJIANG TOBACCO SCIEN CE INSTIT
Filing Date
2025-04-25
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

The lack of effective SNP markers in existing technologies for breeding tobacco wildfire resistance makes it difficult for molecular breeding to meet the needs of rapid selection.

Method used

A SNP marker located at position 111102247 on chromosome 22 of the tobacco genome and its KASP primer set were developed to detect tobacco resistance to race 0 of wildfire. Efficient genotype screening was achieved through PCR amplification and fluorescence signal analysis.

Benefits of technology

It significantly improves breeding efficiency, reduces the cost of field management and phenotypic identification, and can identify 80%-90% of non-target materials through molecular screening at the seedling stage, thereby improving the accuracy and efficiency of breeding.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120464766B_ABST
    Figure CN120464766B_ABST
Patent Text Reader

Abstract

The application discloses a SNP marker and a KASP primer group for detecting the resistance of tobacco wild fire bacteria No. 0 physiological race and application thereof. The application discloses a SNP marker for detecting the resistance of tobacco wild fire bacteria No. 0 physiological race, wherein the SNP marker is located at the position of 111102247 of chromosome No. 22 of a tobacco genome, and the nucleotide type is A or G. The application further discloses a KASP primer group for detecting the SNP marker and application thereof. The SNP marker of the application is a long-flower tobacco resistance site, and 80%-90% of non-target materials can be eliminated through seedling stage molecular screening in the breeding process, so that the labor and material costs of field management and phenotype identification are significantly reduced, and the SNP marker has a wide application prospect.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • CN116649212B

  • CN117327834A

  • CN106884040A

  • KR1018347630000B1