Process for preparing FDP cyclic adenosine monophosphate composite drink by low-temperature enzymolysis method
Through technical means such as low-temperature enzymatic decomposition, gradient complexing and nanodispersion treatment, the problems of prone to inactivation, poor solubility and insufficient stability of active ingredients in functional beverages are solved, and high-quality FDP cyclic adenosine phosphate complex beverages are effectively prepared, which is suitable for physical recovery and cardiovascular health support after high-intensity exercise.
Patent Information
- Application Number
- CN202510739289.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-04
- Publication Date
- 2025-08-26
AI Technical Summary
In the prior art, the preparation process of functional beverages is difficult to balance the degradation of thermally sensitive active ingredients, enzymatic lysis efficiency and product stability. Interactions are prone to occur when the multiphase components are mixed, resulting in turbidity or precipitation. The vitamin B group components have high oxidation sensitivity. The lyophilization process does not support the nano-scale dispersion system, which affects the uniformity and functionality of the product.
Low-temperature enzymatic solution was used to combine nitrogen protection, and directed enzymatic solution was performed using a complex enzyme system of bromelain and trypsin. The pH and temperature were controlled through gradient complexing, combined with three-stage nanodispersion treatment and low-temperature lyophilization process, lecithin was added as a dispersion stabilizer, and atomization spraying technology and nitrogen filling were used to retain the active ingredients and store them in a low-temperature environment.
It significantly improves the yield of bioactive peptides, avoids component inactivation or precipitation, improves product solubility and stability, ensures synergistic efficiency of functional components, and achieves long-term stability and bioavailability of the product.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of nutritional health drinks, and in particular to a process for preparing an FDP cyclic adenosine monophosphate compound drink by a low-temperature enzymatic hydrolysis method. Background Art
[0002] FDP cyclic adenosine monophosphate complex drink is a functional beverage made with fructose 1,6-diphosphate (FDP) as its core functional ingredient, in synergy with adenosine-like substances and various nutrients. As a key intermediate in cellular glucose metabolism, FDP plays a significant role in energy supply, myocardial protection, and improving hypoxia tolerance, while adenosine-like substances also play an important role in cardiovascular regulation and cell signaling. The complex drink aims to achieve nutritional synergy through the scientific combination of red date extract, B vitamins and other natural ingredients. It is suitable for scenarios such as physical recovery after high-intensity exercise and cardiovascular health support, and has high application value.
[0003] In the existing technology, the preparation process of similar functional drinks still faces multiple challenges. Traditional enzymatic hydrolysis processes often lead to degradation of heat-sensitive active ingredients due to improper temperature control, and it is difficult to balance enzymatic hydrolysis efficiency and product stability; when multi-phase ingredients are compounded, substances with different physical and chemical properties are prone to interact with each other, causing turbidity, precipitation or inactivation of active ingredients; in addition, trace components such as B vitamins are highly sensitive to oxidation and are easily lost during processing and storage, affecting the uniformity and functionality of the final product. Conventional freeze-drying processes do not provide sufficient support for nano-scale dispersion systems, which may lead to poor resolubility or reduced bioavailability. Summary of the Invention
[0004] The present invention provides a process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis, which realizes efficient retention of active ingredients, improved system stability and large-scale production through process innovation.
[0005] The technical solution adopted by the present invention is: a process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis, wherein the raw materials are counted by weight: 8 parts of water, 2 parts of red date extract, 0.15 parts of trisodium fructose 1,6-diphosphate, 0.1 parts of deer myocardin powder, 0.1 parts of anhydrous citric acid, 0.004 parts of potassium sorbate, 0.00003 parts of vitamin B1, 0.00003 parts of vitamin B2, and 0.00003 parts of vitamin B6.
[0006] The preparation process includes the following steps:
[0007] S1, pretreatment: the red date extract was treated with 20-40 kHz ultrasound for 10-15 minutes, and then degassed under a vacuum degree of -0.08 to -0.1 MPa;
[0008] S2, low-temperature enzymatic hydrolysis: Deer myocardial peptide powder is mixed with immobilized protease, and enzymatic hydrolysis is carried out at 5-8°C under nitrogen protection for 12-16 hours. After the enzymatic hydrolysis is completed, the protein is instantaneously inactivated at a low temperature of 45-50°C;
[0009] S3, gradient compounding: the product of step S2 is mixed with the pretreated 1,6-diphosphate trisodium salt solution in stages, the mixing speed is controlled at 200-300 rpm, and the whole process is performed in the dark.
[0010] As a further improvement of the present invention, the immobilized protease in step S2 is a composite enzyme system composed of bromelain and trypsin in a mass ratio of 1:2-3, with an enzyme activity unit of 8000-12000 U / g, and the added amount is 0.8-1.5% of the mass of the deer myocardin powder.
[0011] As a further improvement of the present invention, the gradient compounding includes three stages:
[0012] Stage 1: pH 6.6-7.0, temperature 4-6°C, mixing time 30-45 minutes;
[0013] Stage 2: pH 7.1-7.3, temperature 8-10°C, mixing time 20-30 minutes;
[0014] The third stage: pH 6.9-7.1, temperature 2-4°C, mixing time 60-90 minutes,
[0015] The temperature was changed at a rate of 0.5°C / min during the transition between each stage.
[0016] As a further improvement of the present invention, the pretreatment step further comprises: dissolving trisodium fructose-1,6-diphosphate in a buffer solution containing 0.01-0.03% trehalose, filtering through a 0.22 μm microporous membrane, and precooling to 2-4° C. for standby use.
[0017] As a further improvement of the present invention, a freeze-drying step is added after the gradient compounding: the mixed solution is cooled to -40°C at a rate of 1-2°C / min, maintained for 4-6 hours, and then sublimated and dried under vacuum degree ≤10Pa and a heating rate of 0.5°C / min to obtain a freeze-dried powder.
[0018] As a further improvement of the present invention, the freeze-dried powder undergoes a three-stage nano-dispersion treatment: a high-pressure microfluidizer is used to circulate 3-5 times at a pressure of 150-200 MPa; cross-flow filtration is performed through a ceramic membrane with a pore size of 50 nm; and 0.005-0.01% of lecithin is added as a dispersion stabilizer.
[0019] As a further improvement of the present invention, after the gradient compounding is completed, nitrogen microbubbles accounting for 0.5-1% of the total volume are injected, and the product is filled in amber glass bottles under nitrogen-filled conditions and stored at a temperature of ≤-18°C.
[0020] As a further improvement of the present invention, the vitamin B group component is added in the third stage of gradient compounding through an atomization spraying system, the atomization particle size is controlled at 10-20 μm, and the spraying rate is 0.5-1 mL / min.
[0021] Beneficial effects of the present invention: The present invention significantly reduces the degradation of thermosensitive peptides and FDPs through low-temperature enzymatic hydrolysis (5-8°C) combined with nitrogen protection, and the directional enzymatic hydrolysis of the composite enzyme system (bromelain and trypsin) increases the yield of bioactive peptides; in the gradient compounding stage, the pH, temperature and mixing conditions are precisely controlled to avoid inactivation or precipitation caused by multi-component interactions, and combined with the atomization spraying technology of the B vitamins, the biological activity of the oxidation-sensitive components is retained to the greatest extent, achieving synergistic enhancement of the functional ingredients. The innovative use of three-stage nano-dispersion treatment (high-pressure micro-jet homogenization, nano-membrane filtration and lecithin stabilization) makes the freeze-dried powder particle size uniform and the dispersion stable, and there is no stratification after re-dissolution; combined with the low-temperature freeze-drying process (-40°C quick freezing and gradient sublimation drying), a porous loose structure is formed, which improves the dissolution rate and bioavailability. Nitrogen filling and -18°C storage during storage further ensure the long-term stability of the product. DETAILED DESCRIPTION
[0022] In order to make the technical problems, technical solutions and beneficial effects to be solved by this application more clearly understood, this application is further described in detail below in conjunction with the embodiments. It should be understood that the embodiments described herein are only used to explain this application and are not intended to limit this application.
[0023] The present invention provides a process for preparing an FDP cyclic adenosine monophosphate compound drink by a low-temperature enzymatic hydrolysis method. The raw materials comprise, by weight, 8 parts of water, 2 parts of red date extract, 0.15 parts of trisodium fructose 1,6-diphosphate, 0.1 parts of deer myocardin powder, 0.1 parts of anhydrous citric acid, 0.004 parts of potassium sorbate, 0.00003 parts of vitamin B1, 0.00003 parts of vitamin B2, and 0.00003 parts of vitamin B6.
[0024] The preparation process includes the following steps:
[0025] S1, pretreatment: the red date extract was treated with 20-40 kHz ultrasound for 10-15 minutes, and then degassed under a vacuum degree of -0.08 to -0.1 MPa;
[0026] S2, low-temperature enzymatic hydrolysis: Deer myocardial peptide powder is mixed with immobilized protease, and enzymatic hydrolysis is carried out at 5-8°C under nitrogen protection for 12-16 hours. After the enzymatic hydrolysis is completed, the protein is instantaneously inactivated at a low temperature of 45-50°C;
[0027] S3, gradient compounding: the product of step S2 is mixed with the pretreated 1,6-diphosphate trisodium salt solution in stages, the mixing speed is controlled at 200-300 rpm, and the whole process is performed in the dark.
[0028] The immobilized protease in step S2 of the present invention is a composite enzyme system composed of bromelain and trypsin in a mass ratio of 1:2-3, with an enzyme activity unit of 8000-12000 U / g, and the added amount is 0.8-1.5% of the mass of the deer myocardin powder.
[0029] The gradient compounding of the present invention includes three stages:
[0030] Stage 1: pH 6.6-7.0, temperature 4-6°C, mixing time 30-45 minutes;
[0031] Stage 2: pH 7.1-7.3, temperature 8-10°C, mixing time 20-30 minutes;
[0032] The third stage: pH 6.9-7.1, temperature 2-4°C, mixing time 60-90 minutes,
[0033] The temperature was changed at a rate of 0.5°C / min during the transition between each stage.
[0034] The pretreatment step of the present invention further comprises: dissolving trisodium fructose 1,6-diphosphate in a buffer solution containing 0.01-0.03% trehalose, filtering through a 0.22 μm microporous membrane, and precooling to 2-4° C. for standby use.
[0035] After the gradient compounding, a freeze-drying step is added: the mixture is cooled to -40°C at a rate of 1-2°C / min, held for 4-6 hours, and then sublimated and dried under a vacuum of ≤10 Pa and a heating rate of 0.5°C / min to obtain a freeze-dried powder. The freeze-dried powder undergoes a three-stage nanodispersion treatment: 3-5 cycles of high-pressure microfluidizer homogenization at 150-200 MPa; cross-flow filtration through a 50 nm pore size ceramic membrane; and the addition of 0.005-0.01% lecithin as a dispersion stabilizer.
[0036] After the gradient compounding is completed, nitrogen microbubbles accounting for 0.5-1% of the total volume are injected, and the product is filled in amber glass bottles under nitrogen-filled conditions, with a storage temperature of ≤-18°C.
[0037] The vitamin B group component of the present invention is added through an atomization spraying system in the third stage of gradient compounding, the atomization particle size is controlled at 10-20 μm, and the spraying rate is 0.5-1 mL / min.
[0038] Example 1:
[0039] Raw material ratio: 8kg water, 2kg red date extract, 0.15kg trisodium fructose 1,6-diphosphate, 0.1kg deer myocardin powder, 0.1kg anhydrous citric acid, 0.004kg potassium sorbate, 0.03g vitamin B1, 0.03g vitamin B2, 0.03g vitamin B6.
[0040] Step 1: Preprocessing
[0041] A 20% red date extract was ultrasonically processed (frequency: 30 kHz) for 12 minutes, followed by degassing at -0.09 MPa for 20 minutes to remove dissolved oxygen and volatile impurities. Trisodium fructose-1,6-diphosphate was dissolved in phosphate buffer (pH 6.8) containing 0.02% trehalose, filtered through a 0.22 μm microporous membrane, and precooled to 3°C before use.
[0042] Step 2: Low-temperature enzymatic hydrolysis
[0043] Weigh 0.1 kg of deer myocardial peptide powder and mix it with 1.2% immobilized protease (bromelain:trypsin = 1:2.5, enzyme activity 10,000 U / g). Place the mixture in a sealed reactor and purge with nitrogen to an oxygen content of <0.5%. Enzymatic hydrolysis was performed at 6°C for 14 hours, followed by instant inactivation at 48°C for 5 minutes. After inactivation, the mixture was immediately cooled to 4°C.
[0044] Step 3: Gradient compounding
[0045] Stage 1: The enzymatic hydrolysate was mixed with the pretreated FDP solution at 250 rpm, the pH was adjusted to 6.8, and stirred at 5 °C for 40 min;
[0046] Stage 2: Raise the temperature to 9°C (at a rate of 0.5°C / min), adjust the pH to 7.2, and continue mixing for 25 minutes;
[0047] The third stage: cool to 3°C, adjust the pH to 7.0, spray B vitamins (B1, B2, B6) evenly through an atomizing nozzle (particle size 15 μm, rate 0.8 mL / min), and continue mixing for 75 minutes.
[0048] Step 4: Freeze-drying and dispersion
[0049] The compound solution was quickly frozen to -40°C at 1.5°C / min, maintained for 5 hours, and then sublimated and dried for 24 hours at a vacuum of 8 Pa and a heating rate of 0.5°C / min to obtain a freeze-dried powder. The freeze-dried powder was circulated four times by a high-pressure microfluidizer (pressure 180 MPa), then cross-flow filtered through a 50nm ceramic membrane, and finally 0.008% lecithin was added and homogenized to obtain a nano-dispersed powder.
[0050] Step 5: Filling and storage
[0051] Inject 0.8% nitrogen microbubbles into the finished product liquid, fill it into an amber glass bottle (fill with nitrogen until the oxygen content is less than 0.1%), seal it and store it at -20°C.
[0052] Test results
[0053] (1) Activity retention rate: HPLC detection showed that the FDP retention rate was ≥98.5%, and the bioactivity retention rate of deer myocardial peptide was ≥95%;
[0054] (2) Solubility: Reconstitution time of lyophilized powder is less than 15 seconds, and the light transmittance of the solution is greater than 99% (660nm).
[0055] (3) Stability: After storage at -18°C for 6 months, the residual rate of vitamin B1 is ≥92%, with no stratification or precipitation.
[0056] Example 2:
[0057] Raw material ratio: 8kg water, 2kg red date extract, 0.15kg trisodium fructose 1,6-diphosphate, 0.1kg deer myocardin powder, 0.1kg anhydrous citric acid, 0.004kg potassium sorbate, 0.03g vitamin B1, 0.03g vitamin B2, 0.03g vitamin B6.
[0058] Step 1: Preprocessing
[0059] A 20% red date extract was ultrasonically processed (frequency: 35 kHz) for 15 minutes, followed by degassing at -0.1 MPa for 15 minutes. Fructose-1,6-diphosphate trisodium salt was dissolved in Tris-HCl buffer (pH 7.0) containing 0.03% trehalose, filtered through a 0.22 μm microporous membrane, and precooled to 2°C until use.
[0060] Step 2: Low-temperature enzymatic hydrolysis
[0061] Weigh 0.1 kg of deer myocardial peptide powder and mix it with immobilized protease (bromelain:trypsin = 1:3, enzyme activity 12000 U / g) at a ratio of 1.5%. Fill with nitrogen until the oxygen content is less than 0.3%. Enzyme hydrolysis is carried out at a constant temperature of 5°C for 16 hours, followed by instant inactivation at 50°C for 3 minutes. After inactivation, cool to 2°C.
[0062] Step 3: Gradient compounding
[0063] Stage 1: The enzymatic hydrolysate was mixed with the pretreated FDP solution at 300 rpm, the pH was adjusted to 6.6, and stirred at 4 °C for 45 min;
[0064] Stage 2: Raise the temperature to 10°C (at a rate of 0.5°C / min), adjust the pH to 7.3, and mix for 20 minutes;
[0065] The third stage: cooling to 2°C, adjusting the pH to 6.9, spraying B vitamins through an atomizing nozzle (particle size 10 μm, rate 1 mL / min), and mixing for 90 minutes.
[0066] Step 4: Freeze-drying and dispersion
[0067] The reconstituted solution was quickly frozen to -40°C at 2°C / min, maintained for 4 hours, and then sublimation dried for 20 hours at a vacuum of 5 Pa and a heating rate of 0.5°C / min. The lyophilized powder was passed through a high-pressure microfluidizer (pressure 200 MPa) for five cycles, cross-flow filtered through a 50 nm ceramic membrane, and homogenized by adding 0.01% lecithin.
[0068] Step 5: Filling and storage
[0069] Inject 1% nitrogen microbubbles, fill into amber glass bottles (fill with nitrogen until the oxygen content is less than 0.05%), and store at -25°C.
[0070] Test results
[0071] Activity retention rate: FDP retention rate ≥ 99.2%, deer myocardial peptide activity retention rate ≥ 97%;
[0072] Solubility: The reconstitution time of the lyophilized powder is less than 10 seconds, and the viscosity of the solution is reduced to 12mPa·s (25℃);
[0073] Stability: After storage at -25℃ for 6 months, the residual rate of vitamin B2 is ≥94% and the moisture content of the powder is ≤1.5%.
[0074] Example 3:
[0075] Raw material ratio: 8kg water, 2kg red date extract, 0.15kg trisodium fructose 1,6-diphosphate, 0.1kg deer myocardin powder, 0.1kg anhydrous citric acid, 0.004kg potassium sorbate, 0.03g vitamin B1, 0.03g vitamin B2, 0.03g vitamin B6.
[0076] Step 1: Preprocessing
[0077] A 20% red date extract was ultrasonically processed (25 kHz) for 10 minutes, followed by degassing at -0.08 MPa for 25 minutes. Fructose-1,6-diphosphate trisodium salt was dissolved in HEPES buffer (pH 7.2) containing 0.01% trehalose, filtered through a 0.22 μm microporous membrane, and precooled to 4°C until ready for use.
[0078] Step 2: Low-temperature enzymatic hydrolysis
[0079] Weigh 0.1 kg of deer myocardial peptide powder and mix it with 0.8% immobilized protease (bromelain:trypsin = 1:2, enzyme activity 8000 U / g). Nitrogen is then introduced to an oxygen content of <0.6%. Enzymatic hydrolysis is performed at 8°C for 12 hours, followed by instant inactivation at 45°C for 8 minutes. After inactivation, the mixture is cooled to 5°C.
[0080] Step 3: Gradient compounding
[0081] Stage 1: The enzymatic hydrolysate was mixed with the pretreated FDP solution at 200 rpm, the pH was adjusted to 7.0, and stirred at 6 °C for 30 min;
[0082] Stage 2: Raise the temperature to 8°C (at a rate of 0.5°C / min), adjust the pH to 7.1, and mix for 30 minutes;
[0083] The third stage: cooling to 4°C, adjusting the pH to 7.1, spraying B vitamins through an atomizing nozzle (particle size 20 μm, rate 0.5 mL / min), and mixing for 60 minutes.
[0084] Step 4: Freeze-drying and dispersion
[0085] The reconstituted solution was quickly frozen to -40°C at a rate of 1°C / min and maintained for 6 hours. The solution was then sublimated and dried for 28 hours at a vacuum of 10 Pa and a heating rate of 0.5°C / min. The lyophilized powder was passed through a high-pressure microfluidizer (150 MPa) for three cycles, filtered through a 50 nm ceramic membrane, and homogenized by adding 0.005% lecithin.
[0086] Step 5: Filling and storage
[0087] Inject 0.5% nitrogen microbubbles, fill into amber glass bottles (fill with nitrogen until the oxygen content is less than 0.2%), and store at -18°C.
[0088] Test results
[0089] Activity retention rate: FDP retention rate ≥ 97.8%, deer myocardial peptide activity retention rate ≥ 93%;
[0090] Solubility: Reconstitution time of lyophilized powder is less than 18 seconds, and the transmittance of the solution is greater than 98% (660nm);
[0091] Stability: After storage at -18℃ for 6 months, the residual rate of vitamin B6 is ≥90% and the moisture content of the powder is ≤2.0%.
[0092] As can be seen from Examples 1-3 above, the low-temperature enzymatic hydrolysis process for preparing FDP cyclic adenosine monophosphate compound drink provided herein effectively ensures the activity, solubility, and stability of each component in the product. While the specific parameters of the different examples vary, all achieve good results. This demonstrates the flexibility and adaptability of the process, allowing for appropriate adjustments based on actual production needs and conditions to meet the quality requirements of the FDP cyclic adenosine monophosphate compound drink in different scenarios.
[0093] In summary, the present invention's low-temperature enzymatic hydrolysis process for preparing FDP cyclic adenosine monophosphate (cAMP) compound beverage successfully addresses key issues associated with the preparation of FDP cyclic adenosine monophosphate compound beverages, including the inactivation of active ingredients, poor solubility, and insufficient stability, through a series of scientifically rational and coordinated steps, including pretreatment, low-temperature enzymatic hydrolysis, gradient compounding, freeze-drying and dispersion processing, and filling and storage. This process fully leverages the unique advantages of each step. For example, precise control of temperature, nitrogen protection, and the enzyme system during low-temperature enzymatic hydrolysis effectively improves the yield of bioactive peptides. The ingenious use of pH, temperature, mixing conditions, and atomization spraying technology during the gradient compounding stage achieves synergistic enhancement of the functional ingredients. The combination of a three-stage nano-dispersion process and low-temperature freeze-drying fundamentally improves the product's particle size uniformity, dispersion stability, dissolution rate, and bioavailability. Furthermore, nitrogen-filled filling and low-temperature storage further ensure the product's long-term stability, laying a solid foundation for the large-scale industrial production of FDP cyclic adenosine monophosphate compound beverages and promising application prospects in the health beverage market.
[0094] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis, characterized in that: The raw material composition is counted by weight: 8 parts of water, 2 parts of red date extract, 0.15 parts of trisodium fructose 1,6-diphosphate, 0.1 parts of deer myocardin powder, 0.1 parts of anhydrous citric acid, 0.004 parts of potassium sorbate, 0.00003 parts of vitamin B1, 0.00003 parts of vitamin B2, and 0.00003 parts of vitamin B6. The preparation process includes the following steps: S1, pretreatment: the red date extract was treated with 20-40 kHz ultrasound for 10-15 minutes, and then degassed under a vacuum degree of -0.08 to -0.1 MPa; S2, low-temperature enzymatic hydrolysis: Deer myocardial peptide powder is mixed with immobilized protease, and enzymatic hydrolysis is carried out at 5-8°C under nitrogen protection for 12-16 hours. After the enzymatic hydrolysis is completed, the protein is instantaneously inactivated at a low temperature of 45-50°C; S3, gradient compounding: the product of step S2 is mixed with the pretreated 1,6-diphosphate trisodium salt solution in stages, the mixing speed is controlled at 200-300 rpm, and the whole process is performed in the dark.
2. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 1, characterized in that: In step S2, the immobilized protease is a composite enzyme system composed of bromelain and trypsin in a mass ratio of 1:2-3, with an enzyme activity unit of 8000-12000 U / g, and the added amount is 0.8-1.5% of the mass of the deer myocardin powder.
3. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 1, characterized in that: The gradient compounding includes three stages: Stage 1: pH 6.6-7.0, temperature 4-6°C, mixing time 30-45 minutes; Stage 2: pH 7.1-7.3, temperature 8-10°C, mixing time 20-30 minutes; The third stage: pH 6.9-7.1, temperature 2-4°C, mixing time 60-90 minutes, The temperature was changed at a rate of 0.5°C / min during the transition between each stage.
4. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 1, characterized in that: The pretreatment step further comprises: dissolving trisodium fructose 1,6-diphosphate in a buffer solution containing 0.01-0.03% trehalose, filtering through a 0.22 μm microporous membrane, and precooling to 2-4° C. for later use.
5. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 1, characterized in that: A freeze-drying step is added after the gradient compounding: the mixed solution is cooled to -40°C at a rate of 1-2°C / min, maintained for 4-6 hours, and then sublimated and dried under vacuum degree ≤10Pa and a heating rate of 0.5°C / min to obtain freeze-dried powder.
6. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 5, characterized in that: The freeze-dried powder is subjected to three-stage nano-dispersion treatment: a high-pressure microfluidizer is used to circulate 3-5 times at a pressure of 150-200 MPa; cross-flow filtration is performed through a ceramic membrane with a pore size of 50 nm; and 0.005-0.01% of the total amount of lecithin is added as a dispersion stabilizer.
7. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 1, characterized in that: After the gradient compounding is completed, nitrogen microbubbles accounting for 0.5-1% of the total volume are injected, and the product is filled in amber glass bottles under nitrogen-filled conditions. The storage temperature is ≤-18°C.
8. The process for preparing FDP cyclic adenosine monophosphate compound drink by low-temperature enzymatic hydrolysis according to claim 1, characterized in that: The vitamin B group components are added in the third stage of gradient compounding through an atomization spraying system, the atomization particle size is controlled at 10-20 μm, and the spraying rate is 0.5-1 mL / min.