Sour pomegranate seed extract and application thereof in whitening skin care products

Abstract

The invention belongs to the field of daily chemicals, and particularly relates to a sour pomegranate seed extract and application thereof in whitening and skin care products. The preparation method of the sour pomegranate seed extract comprises the following steps: soaking sour pomegranate seeds in a lactic acid bacteria solution, and fermenting under a sealed condition; adding the fermented sour pomegranate seeds into liquid nitrogen for quick freezing, then performing freeze drying, and grinding into powder; the preparation method comprises the following steps: adding dried sour pomegranate seed powder into weakly alkaline water, treating with a colloid mill to obtain a suspension, performing ultrasonic-assisted extraction and ultrasonic treatment, adding a compound enzyme for enzymolysis, treating the solution after enzymolysis in an electric field, adsorbing with activated carbon, and filtering to obtain the sour pomegranate seed extract. The sour pomegranate seed extract disclosed by the invention has a relatively good whitening effect.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular relates to an acid pomegranate seed extract and application thereof in whitening and skin care products. Background Art

[0002] As the largest organ on the human body's surface, the skin performs multiple functions, including barrier function, thermoregulation, and immunity. Due to its long-term contact with the external environment, the skin is significantly affected by these factors. Ultraviolet radiation, radiation from electronic products, and unhealthy eating and living habits all place a significant burden on the skin. In recent years, with the improvement of living standards, people's pursuit of beauty has become increasingly sophisticated, and the demand for cosmetics has also increased. A fair and translucent complexion is a long-cherished goal for many women. With the advancement of modern technology, a variety of cosmetics have emerged. Currently, many whitening products are available on the market. While they offer strong whitening effects, they are also highly irritating and may damage the skin barrier.

[0003] Pomegranate seeds are a byproduct of pomegranate processing, accounting for approximately 12% of the total weight of the fruit. They are rich in nutrients such as cellulose, fatty acids, protein, and carbohydrates, as well as physiologically active ingredients such as polyphenols, flavonoids, and anthocyanins, which are beneficial to the human body. Currently, research on pomegranate seeds, both domestically and internationally, has focused on pomegranate seed oil. It has been found that pomegranate seed oil contains a variety of fatty acids, primarily oleic acid, palmitic acid, and punicic acid, among other unsaturated fatty acids, exhibiting antioxidant, anti-tumor, and lipid-regulating activities. However, the application of pomegranate seed extract in skin whitening and skincare has not been publicly reported. Summary of the Invention

[0004] The present invention aims to provide an acid pomegranate seed extract and application thereof in whitening and skin care products.

[0005] To achieve the purpose of the present invention, the technical solution adopted by the present invention is:

[0006] A method for preparing a pomegranate seed extract comprises the following steps: immersing the pomegranate seeds in a lactic acid bacteria solution and fermenting the solution under sealed conditions; adding liquid nitrogen to the fermented pomegranate seeds for quick freezing, freeze-drying the solution, and then grinding the solution into powder; adding the dried pomegranate seed powder into weakly alkaline water, treating the solution with a colloid mill to obtain a suspension, performing ultrasound-assisted extraction, adding a complex enzyme after the ultrasound treatment to perform enzymolysis, treating the solution after the enzymolysis in an electric field, adsorbing the solution with activated carbon, and filtering the solution to obtain the pomegranate seed extract.

[0007] Furthermore, the mass ratio of the dried acid pomegranate seed powder to the weakly alkaline water is 1:3-4; the weakly alkaline water is adjusted to a pH of 8-9 using sodium citrate.

[0008] Furthermore, the complex enzyme includes cellulase, pectinase and ligninase; the ratio of the cellulase, pectinase and ligninase is 5:2:3-4.

[0009] Furthermore, the ultrasonic treatment steps are as follows: the ultrasonic frequency is maintained at 40 kHz for 40 minutes, increased to 80 kHz for 40 minutes, and then adjusted to 40 kHz for 40 minutes.

[0010] Furthermore, the grinding speed of the colloid mill is 1600-1800 rpm.

[0011] Furthermore, the electric field adopts a constant direct current or a pulsed direct current, and the treatment time is 2 to 4 minutes.

[0012] The present invention also provides an acid pomegranate seed extract prepared by the above method.

[0013] The present invention also provides application of the acid pomegranate seed extract in daily chemical products.

[0014] Furthermore, the daily chemical product is a whitening skin care product.

[0015] Furthermore, the mass ratio of the acid pomegranate seed extract added to the whitening skin care product is 0.01 to 10%.

[0016] Beneficial effects

[0017] The invention uses the acid pomegranate seeds as raw materials and can efficiently extract active ingredients such as polyphenols and flavonoids from the acid pomegranate seeds through multiple extraction steps, thereby achieving the effect of fully and effectively utilizing the acid pomegranate seeds and reducing waste.

[0018] The acid pomegranate seeds are processed in a liquid nitrogen quick-freezing manner, so that the surface and internal moisture of the acid pomegranate seeds can be quickly formed into ice crystals, rapidly destroying the cell membrane, cell wall and epidermal tissue, which is conducive to the dissolution of active substances in the subsequent extraction process; and because the acid pomegranate seeds have a larger hardness, after liquid nitrogen quick-freezing and freeze-drying, the acid pomegranate seeds are easier to crush; by subjecting the solution to electric field treatment under specific conditions, the permeability of the acid pomegranate seed cells can be improved, and the outflow of intracellular active substances can be promoted, so as to obtain better extraction effect. The present invention is processed by using ultrasound-assisted enzymolysis and then placed in an electric field, which can effectively improve the extraction rate of active ingredients.

[0019] The acid pomegranate seed extract of the present invention regulates the exudation of hydrated compounds by changing keratin tissue, reabsorbing water and forming hydrates in the epidermis of the skin, thereby hydrating the skin; superoxide dismutase can remove excess free radicals from the human body, has the effects of delaying skin aging, anti-oxidation, and removing spots, and can promote the generation of aquaporins, which is beneficial to the maintenance of moisture on the skin surface. The two have a synergistic technical effect, so that the prepared skin care product has an excellent hydration and water-locking effect. By comparing the examples and comparative examples, it is proved that the skin care product prepared by the present invention has a good whitening effect. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be described below.

[0021] Example 1

[0022] The method comprises the following steps: immersing the acid pomegranate seeds in a lactic acid bacteria liquid, placing the mixture under sealed high-humidity conditions for fermentation for 10 to 14 hours, wherein the fermentation temperature is 40 to 43° C. and the humidity is 85 to 90%. The fermented acid pomegranate seeds are quickly frozen by adding liquid nitrogen, freeze-dried, and then ground into powder. The dried acid pomegranate seed powder is added into weakly alkaline water and treated with a colloid mill to obtain a suspension. The suspension is subjected to ultrasound-assisted extraction for 2 to 3 hours. After the ultrasound treatment, a complex enzyme is added for enzymatic hydrolysis. The enzymatic hydrolysis solution is subjected to treatment in an electric field, adsorbed with activated carbon, and filtered to obtain the acid pomegranate seed extract.

[0023] The mass ratio of dried acid pomegranate seed powder to weakly alkaline water is 1:3-4. The complex enzyme comprises cellulase, pectinase, and ligninase, with the ratio of cellulase, pectinase, and ligninase being 5:2:3-4. The weakly alkaline water is adjusted to a pH of 8-9 using sodium citrate. The ultrasonic treatment steps include maintaining an ultrasonic frequency of 40 kHz for 40 minutes, increasing it to 80 kHz for 40 minutes, and then adjusting it to 40 kHz for 40 minutes. The grinding speed of the colloid mill is 1600-1800 rpm. The electric field uses a constant direct current or a pulsed direct current, and the treatment time is 2-4 minutes.

[0024] The pomegranate seed extract was added to the body lotion at a mass ratio of 1.0%, and superoxide dismutase was added at a mass ratio of 0.2%. The formula of the body lotion was as follows:

[0025]

[0026]

[0027] Example 2

[0028] The pomegranate seed extract was added to the facial essence at a mass ratio of 1.0%, and superoxide dismutase was added at a mass ratio of 0.2%. The formula of the essence was as follows:

[0029]

[0030]

[0031] Comparative Example 1

[0032] This comparative example provides a body lotion, which differs from the comparative document 1 in that it does not contain the acid pomegranate seed extract described in Example 1, and the other conditions are exactly the same as those in Example 1.

[0033] Comparative Example 2

[0034] This comparative example provides a body lotion, which differs from the comparative document 1 in that the preparation method of the acid pomegranate seed extract does not include a fermentation step, and the other conditions are exactly the same as those in Example 1.

[0035] Comparative Example 3

[0036] This comparative example provides a body lotion, which differs from the comparative document 1 in that the preparation method of the acid pomegranate seed extract does not include a liquid nitrogen quick-freezing step, and the other conditions are exactly the same as those in Example 1.

[0037] Comparative Example 4

[0038] This comparative example provides a body lotion, which differs from the comparative document 1 in that, in the preparation method of the acid pomegranate seed extract, freeze-drying is adjusted to drying, and the drying temperature is 50-60°C. The other conditions are exactly the same as those in Example 1.

[0039] Comparative Example 5

[0040] This comparative example provides a body lotion, which differs from comparative document 1 in that the weakly alkaline water in the acid pomegranate seed extract is replaced with deionized water, and the other conditions are exactly the same as those in Example 1.

[0041] Comparative Example 6

[0042] This comparative example provides a body lotion, which differs from the comparative document 1 in that the preparation method of the acid pomegranate seed extract does not include colloid milling treatment, and the other conditions are exactly the same as those in Example 1.

[0043] Comparative Example 7

[0044] This comparative example provides a body lotion, which differs from the comparative document 1 in that the preparation method of the acid pomegranate seed extract does not include an ultrasonic step, and the other conditions are exactly the same as those in Example 1.

[0045] Comparative Example 8

[0046] This comparative example provides a body lotion, which differs from the comparative document 1 in that the preparation method of the acid pomegranate seed extract does not include an enzymatic hydrolysis step, and the other conditions are exactly the same as those in Example 1.

[0047] Comparative Example 9

[0048] This comparative example provides a body lotion, which differs from the comparative document 1 in that the preparation method of the acid pomegranate seed extract does not include an electric field treatment step, and the other conditions are exactly the same as those in Example 1.

[0049] Comparative Example 10

[0050] This comparative example provides a body lotion, which differs from the comparative document 1 in that the body lotion does not contain superoxide dismutase, and the other conditions are exactly the same as those in Example 1.

[0051] Comparative Example 11

[0052] This comparative example provides a body lotion, which differs from the comparative document 1 in that the body lotion does not contain pomegranate seed extract and superoxide dismutase, and the other conditions are exactly the same as those in Example 1.

[0053] Example 3

[0054] In order to verify the mildness of the body lotion of the present invention, each embodiment and comparative example was subjected to a skin irritation test: healthy white rabbits were selected as experimental animals, with 3 rabbits in each group. Before the test, the hair on both sides of the back spine was trimmed to ensure that there was no epidermal damage. The hair removal area was approximately 3cm×3cm on each side. 0.5mL was applied to the rabbit skin. After 30 minutes, the residual test substance was removed with water. The rabbits were observed for erythema, edema, etc. 1 hour and 24 hours after washing. The skin irritation intensity was determined based on the table in Chapter 6, Toxicology Test Method 4, Skin Irritation / Corrosion Test of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0055] Table 1

[0056]

[0057]

[0058] As shown in Table 1, the product of Example 1 is non-irritating and has a good formula safety, but some of the ingredients are irritating to the skin.

[0059] Example 4

[0060] A 1 g / L L-dopa solution was prepared using a 0.2 mol / L phosphate buffer solution (pH 6.8); a 100 U / mL tyrosinase solution was prepared using a 0.2 mol / L phosphate buffer solution (pH 6.8); a 2 mg / mL test solution containing the essence of the embodiment or comparative example was prepared using a 0.2 mol / L phosphate buffer solution (pH 6.8); 40 μL of the L-dopa solution, 40 μL of the test solution and / or 4 0 μL of tyrosinase solution was added to a 96-well plate, followed by reaction in a 30°C water bath for 40 minutes; after the reaction was completed, the absorbance was measured at 490 nm, and each experiment was repeated 3 times. The absorbance of each experiment was the average of the 3 tests to obtain absorbance C; referring to the above method, phosphate buffer was used instead of tyrosinase solution, and the absorbance was D; phosphate buffer was used instead of the test solution, and the absorbance was A; phosphate buffer was used instead of the test solution and tyrosinase solution, and the absorbance was B.

[0061] Tyrosinase activation rate (%) = [(CD)-(AB)] / (AB)*100%

[0062] The higher the tyrosinase activation rate, the stronger the ability to promote melanin production, which means the better the whitening effect. Specific test data are shown in the table below.

[0063] The samples prepared in the examples and comparative examples were tested for tyrosinase inhibitory activity, and the results were as follows:

[0064]

[0065]

[0066] Tyrosinase is the rate-limiting enzyme in melanin production, and the whitening performance of cosmetics is usually reflected by measuring the tyrosinase inhibition rate. The above test results show that the body lotion of Example 1 of the present invention has a significant tyrosinase inhibition effect and has a good whitening effect.

[0067] Example 5

[0068] Volunteers aged between 20 and 50 with healthy skin were randomly divided into groups. The flat area on the inner side of the left forearm of the volunteers was used as the test area. Ultraviolet radiation was irradiated using an artificial sun irradiator at a dose of 1.2 MED. The irradiated area was a circular area with a diameter of about 0.8 cm. The body lotions of the above embodiment and comparative example were used respectively. The application method was to use the body lotion once in the morning and once in the afternoon every day, with a dosage of about 2 mg / cm 2 , lasting for 4 weeks. The skin melanin value of the test area was tested with a skin melanin tester before and 4 weeks after using the product. The measurement was performed three times, and the average result was taken. The melanin change rate after irradiation was calculated. The results are as follows:

[0069]

[0070]

[0071] As can be seen from the table above, the body lotion prepared in Example 1 significantly reduced the MI value and melanin content in the skin. While the body lotion prepared in the comparative example did reduce the MI value, the reduction was smaller, and the melanin content in the skin remained high. Research has shown that this is due to the acidic pomegranate seed extract prepared by the method of Example 1, which reduces melanin synthesis, prevents melanin precipitation and aggregation, and phagocytizes melanin, resulting in the excellent whitening effect of the resulting composition.

[0072] Example 6

[0073] Volunteers aged between 20 and 50 years old with healthy skin were selected and randomly divided into groups. The 4cm×4cm area on the inner side of the left forearm of the volunteers was used as the test area. The body lotions of the above embodiments and comparative examples were used respectively. During the experiment, the volunteers were not allowed to apply any cosmetics on the experimental area. The method of use was to use it once in the morning and once in the evening for 4 weeks. Before each test, the test area was cleaned and sat in a laboratory with a temperature of 21±1°C and a humidity of 50±5%RH for 30 minutes. The skin moisture content was detected with a skin moisture tester before and 4 weeks after using the product. The test was conducted three times, and the results were averaged. The rate of change in moisture content before and 4 weeks after using the product was calculated. The results are as follows:

[0074]

[0075]

[0076] As can be seen from the table above, the body lotion using Example 1 effectively increases the moisture content of the skin, while the body lotion using the comparative example, while showing some increase in moisture content, does so at a smaller rate. Research has found that the acidic pomegranate seed extract described herein regulates the exudation of hydrated compounds by altering keratin structure, reabsorbing water and forming hydrates in the epidermis, thereby hydrating the skin. Superoxide dismutase can scavenge excess free radicals in the human body, delaying skin aging, providing antioxidant benefits, and removing freckles. It also promotes the production of aquaporins, facilitating surface moisture retention. These two effects create a synergistic, synergistic effect, resulting in a body lotion with excellent hydration and moisture retention.

Claims

1. A method for preparing an acidic pomegranate seed extract, characterized in that: The following steps are involved: The method comprises the following steps: immersing the acid pomegranate seeds in a lactic acid bacteria liquid and fermenting them under sealed conditions; adding liquid nitrogen to the fermented acid pomegranate seeds for quick freezing, freeze-drying the seeds, and then grinding the seeds into powder; adding the dried acid pomegranate seed powder into weakly alkaline water and treating the powder with a colloid mill to obtain a suspension, performing ultrasound-assisted extraction, adding a complex enzyme for enzymatic hydrolysis after the ultrasonic treatment, treating the solution after the enzymatic hydrolysis in an electric field, adsorbing the solution with activated carbon, and filtering the solution to obtain the acid pomegranate seed extract.

2. The method for preparing the acid pomegranate seed extract according to claim 1, wherein The mass ratio of the dried acid pomegranate seed powder to weak alkaline water is 1:3-4; the pH value of the weak alkaline water is adjusted to 8-9 by using sodium citrate.

3. The method for preparing the acid pomegranate seed extract according to claim 1, wherein The complex enzyme comprises cellulase, pectinase and ligninase; the ratio of the cellulase, pectinase and ligninase is 5:2:3-4.

4. The method for preparing the acid pomegranate seed extract according to claim 1, wherein The ultrasonic treatment steps are as follows: ultrasonic frequency is maintained at 40 kHz for 40 min, increased to 80 kHz and maintained for 40 min, and then adjusted to 40 kHz and maintained for 40 min.

5. The method for preparing the acid pomegranate seed extract according to claim 1, wherein The grinding speed of the colloid mill is 1600-1800 rpm.

6. The method for preparing the acid pomegranate seed extract according to claim 1, wherein The electric field adopts a constant direct current or a pulsed direct current, and the treatment time is 2 to 4 minutes.

7. An acid pomegranate seed extract, characterized in that Prepared by the method of claim 1.

8. Use of the acid pomegranate seed extract according to claim 7 in daily chemical products.

9. The use according to claim 8, characterized in that The daily chemical product is a whitening skin care product.

10. The use according to claim 9, characterized in that The mass ratio of the acid pomegranate seed extract added to the whitening skin care product is 0.01-10%.

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