Silver ion polypeptide antibacterial gel

Abstract

The invention relates to the technical field of health care products, in particular to silver ion polypeptide antibacterial gel. The invention discloses a silver-polypeptide-containing shampoo, which is prepared from the following ingredients including 0.1 to 1 percent of silver ions, 0.5 to 3 percent of polypeptide, 60 to 85 percent of deionized water, 1 to 5 percent of jingnaoji leaf extracts, 1 to 5 percent of herba portulacae extracts, 1 to 5 percent of radix notoginseng extracts, 1 to 5 percent of radix sophorae flavescentis extracts, 1 to 5 percent of saffron crocus extracts, 1 to 5 percent of apple seed extracts, 1 to 5 percent of herba violae extracts, 1 to 5 percent of fructus cnidii extracts, 1 to 5 percent of sunflower extracts, 1 to 5 percent of herba centellae extracts and 0.5 to 3 percent of herba menthae extracts. The preparation method comprises the following steps: extraction preparation, silver ion preparation, mixing and stirring, and gel preparation. The invention has the beneficial effects of scientific and reasonable formula, obvious synergistic effect and strong antibacterial effect; the preparation process is simple and feasible, effective activity of each component can be fully reserved, and stable product quality is guaranteed; the product is mild and non-irritant, is suitable for daily cleaning, health care and health care of private parts, does not destroy normal microbial balance of the private parts, and is high in use safety.

Description

Technical Field

[0001] The invention relates to the technical field of sanitary care products, in particular to a silver ion polypeptide antibacterial gel. Background Art

[0002] Currently, a wide variety of hygiene products are available to treat genital discomfort caused by Staphylococcus aureus, Candida albicans, and Escherichia coli. However, some products suffer from poor antibacterial efficacy and irritating ingredients. Some traditional antibacterial products may disrupt the normal microbial balance in the genital area, impacting genital health. Therefore, developing a hygiene product that is highly effective in inhibiting bacteria, gentle and non-irritating, and provides comprehensive care is crucial. Summary of the Invention

[0003] In order to overcome the technical defects in the prior art and effectively solve the technical problems in the background technology, the present invention provides the following technical solutions:

[0004] A silver ion polypeptide antibacterial gel is composed of the following ingredients: 0.1-1% silver ions, 0.5-3% polypeptides, 60-85% deionized water, 1-5% Thuja chinensis leaf extract, 1-5% Portulaca oleracea extract, 1-5% Panax notoginseng root extract, 1-5% Sophora flavescens extract, 1-5% Saffron extract, 1-5% Apple seed extract, 1-5% Atractylodes lancea extract, 1-5% Cnidium monnieri extract, 1-5% Sunflower extract, 1-5% Centella asiatica extract, and 0.5-3% peppermint extract.

[0005] As a preferred technical solution of the present invention, the invention is composed of the following ingredients: 0.1% silver ions, 3% polypeptide, 60% deionized water, 5% Thuja chinensis leaf extract, 1% Portulaca oleracea extract, 5% Panax notoginseng root extract, 1% Sophora flavescens extract, 5% Saffron extract, 1% Apple seed extract, 5% Atractylodes lancea extract, 1% Cnidium monnieri extract, 5% Sunflower extract, 1% Centella asiatica extract, and 3% Peppermint extract.

[0006] As a preferred technical solution of the present invention, the invention is composed of the following ingredients: 1% silver ions, 0.5% polypeptide, 85% deionized water, 1% Thuja chinensis leaf extract, 5% Portulaca oleracea extract, 1% Panax notoginseng root extract, 5% Sophora flavescens extract, 1% Saffron extract, 5% Apple seed extract, 1% Atractylodes lancea extract, 5% Cnidium monnieri extract, 1% Sunflower extract, 5% Centella asiatica extract, and 0.5% Peppermint extract.

[0007] As a preferred technical solution of the present invention, the invention is composed of the following ingredients: 0.5% silver ions, 2.5% polypeptide, 75% deionized water, 3% Thuja chinensis leaf extract, 3.5% Portulaca oleracea extract, 2.5% Panax notoginseng root extract, 4.5% Sophora flavescens extract, 3% Saffron extract, 3.5% Apple seed extract, 2.5% Atractylodes lancea extract, 4.5% Cnidium monnieri extract, 3% Sunflower extract, 3.5% Centella asiatica extract, and 1.8% Peppermint extract.

[0008] A method for preparing a silver ion polypeptide antibacterial gel comprises the following steps: S1: extraction and preparation:

[0009] Thuja chinensis leaves, purslane, notoginseng root, sophora flavescens, saffron, apple seeds, radix ruticosae, cnidium monnieri, sunflower, centella asiatica, and mint were pretreated to remove impurities, and then extracted 2-3 times at 40-60° C. with an ethanol-water mixture as the extraction solvent, each extraction time for 1-2 hours, and the extracts were combined and concentrated under reduced pressure to obtain the plant extracts for later use;

[0010] S2: Preparation of silver ions: Using a chemical reduction method, silver nitrate solution and a reducing agent are mixed in a certain proportion, and reacted at 50-70°C for 1-2 hours under stirring to obtain a silver ion solution;

[0011] S3: Mixing and stirring: Add deionized water to the reactor, turn on the stirrer, control the temperature at 30-40°C, add the above-prepared plant extracts, silver ion solution and polypeptide in sequence, and continue stirring for 30-60 minutes to fully mix the ingredients;

[0012] S4: Gel preparation: Add gel matrix to the above mixed solution, continue stirring until a uniform gel is formed, adjust the pH value to 5.5-7.0, let it stand for defoaming, and obtain silver ion polypeptide antibacterial gel.

[0013] As a preferred technical solution of the present invention, the reducing agent in step S2 is sodium citrate solution, and the mixing ratio of the silver nitrate solution to the reducing agent is 1:1.5-2.5.

[0014] As a preferred technical solution of the present invention, the gel matrix in step S4 is carbomer.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. The formula of the silver ion polypeptide antibacterial gel of the present invention is scientific and reasonable. It combines the high-efficiency antibacterial property of silver ions, the restorative property of polypeptides with the antibacterial and anti-inflammatory effects of various natural plant extracts. It has a significant synergistic effect and has a strong inhibitory effect on Candida albicans, Staphylococcus aureus and Escherichia coli.

[0017] 2. The preparation process is simple and feasible. The extraction method and preparation technology adopted can fully retain the effective activity of each component and ensure stable product quality.

[0018] 3. The product is mild and non-irritating, suitable for daily cleaning and hygiene care of the vagina. It will not destroy the normal microbial balance of the vagina and is highly safe to use.

[0019] In the present invention:

[0020] 1. Medicinal properties and efficacy of each component:

[0021] Silver ions:

[0022] From the perspective of modern medicine, silver ions have broad-spectrum and highly effective antibacterial and bactericidal properties. They can penetrate bacterial cell membranes, combine with proteins, nucleic acids and other biological macromolecules in the cells, destroy their structure and function, and thus inhibit the growth and reproduction of Staphylococcus aureus, Candida albicans, Escherichia coli and other pathogens. They are the core component of the product to achieve antibacterial function.

[0023] Peptides:

[0024] Peptides have excellent biocompatibility, promoting cell proliferation and differentiation, and accelerating the repair and regeneration of damaged tissue. In vaginal care, they can help repair tissue damage caused by bacterial infection or inflammation, enhancing the resistance and self-healing ability of local tissues.

[0025] Thuja chinensis leaf extract:

[0026] Medicinal properties: bitter taste, cool nature.

[0027] Efficacy: It is often used in traditional medicine to clear away heat and detoxify, astringe and stop bleeding; modern research shows that it contains a variety of flavonoids and phenolic compounds, which have significant antibacterial, anti-inflammatory and antioxidant activities, can inhibit the growth of pathogens and reduce local inflammatory reactions.

[0028] Purslane Extract:

[0029] Medicinal properties: sour, cold in nature.

[0030] Efficacy: It has the effects of clearing away heat and detoxifying, cooling blood and stopping bleeding, and dispersing blood stasis and reducing swelling. Rich in alkaloids and flavonoids, it has a strong inhibitory effect on Escherichia coli and Staphylococcus aureus, and can also relieve discomfort such as swelling and pain in the genitals.

[0031] Panax notoginseng root extract:

[0032] Medicinal properties: sweet, slightly bitter, warm in nature.

[0033] Efficacy: It has the effects of dispersing blood stasis, stopping bleeding, reducing swelling and relieving pain. Notoginseng saponins and other ingredients it contains can promote blood circulation, improve the nutritional supply of local tissues, accelerate the disappearance of inflammation, and promote the repair of damaged tissues.

[0034] Sophora flavescens extract:

[0035] Medicinal properties: bitter in taste, cold in nature.

[0036] Efficacy: Clears away heat and dampness, dispels wind and kills insects. The alkaloids it contains, such as matrine, have a strong inhibitory effect on Candida albicans and Staphylococcus aureus, and can also relieve symptoms such as genital itching.

[0037] Saffron Extract:

[0038] Medicinal properties: sweet, neutral in nature.

[0039] Efficacy: Activates blood circulation, removes blood stasis, cools blood and detoxifies. It can improve genital blood circulation, promote metabolism, and enhance local tissue immunity. At the same time, its ingredients such as crocin have certain antibacterial and anti-inflammatory effects.

[0040] Apple Seed Extract:

[0041] Medicinal properties: sweet, slightly bitter, neutral in nature.

[0042] Efficacy: Rich in antioxidants such as polyphenols, flavonoids, etc., it has antioxidant and anti-inflammatory effects, can protect cells from free radical damage, enhance tissue resistance, and help inhibit pathogens.

[0043] Purple Flower Extract:

[0044] Medicinal properties: spicy, bitter, cold in nature.

[0045] Efficacy: Clears away heat and detoxifies, cools blood and reduces swelling. It has an inhibitory effect on a variety of pathogens, can reduce genital inflammation and relieve discomfort.

[0046] Cnidium monnieri extract:

[0047] Medicinal properties: spicy, bitter, warm in nature.

[0048] Efficacy: Warming the kidneys and strengthening yang, drying dampness and dispelling wind, killing insects and relieving itching. The coumarins it contains have a strong inhibitory effect on fungi such as Candida albicans and are commonly used to treat genital itching and inflammation.

[0049] Sunflower Extract:

[0050] Medicinal properties: sweet, neutral in nature.

[0051] Efficacy: Contains a variety of vitamins and flavonoids, has anti-inflammatory, antioxidant and immunomodulatory effects, can enhance the resistance of local tissues and promote tissue repair.

[0052] Centella asiatica extract:

[0053] Medicinal properties: bitter, spicy, cold in nature.

[0054] Efficacy: Clears away heat and dampness, detoxifies and reduces swelling, and promotes skin regeneration. Ingredients such as asiatica glycosides it contains can accelerate wound healing and repair damaged tissues, while also having anti-inflammatory and antibacterial effects.

[0055] Peppermint Extract:

[0056] Medicinal properties: spicy, cool in nature.

[0057] Efficacy: It has the effects of cooling and relieving itching, dispersing wind and dissipating heat. It can relieve uncomfortable symptoms such as itching and burning in the vagina, giving the user a cool and comfortable feeling.

[0058] 2. Analysis of monarch, minister, assistant and envoy combinations:

[0059] King medicine:

[0060] Silver ions and peptides serve as the main ingredients. Silver ions, with their powerful broad-spectrum antibacterial and bactericidal properties, directly target pathogenic bacteria like Staphylococcus aureus, Candida albicans, and Escherichia coli, inhibiting their growth at the source and providing the key to resolving vaginal infections. Peptides, on the other hand, focus on repairing damaged tissue. The two complement each other, jointly addressing vaginal discomfort and laying the foundation for the product's core efficacy.

[0061] Minister medicine:

[0062] Thuja chinensis leaf extract, Portulaca oleracea extract, Sophora flavescens extract, and Cnidium monnieri extract serve as auxiliary herbs. They complement the main herbs in different ways, enhancing their antibacterial effects. Thuja chinensis leaf extract, Portulaca oleracea extract, and Sophora flavescens extract have significant inhibitory effects against a wide range of pathogens, broadening their antibacterial spectrum and synergizing with silver ions for more comprehensive bacterial suppression. Cnidium monnieri extract has a strong antifungal effect, enhancing its effectiveness against fungal infections such as Candida albicans. Its kidney-warming and dampness-removing properties also help improve the overall genital environment, enhancing therapeutic efficacy.

[0063] Adjuvant:

[0064] Panax notoginseng root extract, saffron extract, apple seed extract, Amaranthus chinensis extract, sunflower extract, and Centella asiatica extract serve as adjuvants. Panax notoginseng root extract and saffron extract promote blood circulation by promoting blood circulation and removing blood stasis, providing a favorable nutritional environment for tissue repair and assisting peptides in promoting the repair of damaged tissues. Apple seed extract, Amaranthus chinensis extract, sunflower extract, and Centella asiatica extract possess antioxidant, anti-inflammatory, and immunomodulatory properties, enhancing tissue resistance while synergizing with other ingredients to reduce inflammatory responses and promote recovery. They also reduce potential irritation from the main and auxiliary herbs, making the formula more gentle.

[0065] Drug:

[0066] Peppermint extract is the adjuvant. Its cooling and antipruritic properties can quickly relieve uncomfortable symptoms such as genital itching and burning, enhancing the user experience. Peppermint extract also promotes transdermal absorption, guiding other drug ingredients to penetrate the genital tissue better, enhancing the drug's effects and allowing the entire formula to function more effectively.

[0067] Through the exquisite combination of monarch, minister, assistant and guiding ingredients, the various ingredients in this formula work together and complement each other, which not only ensures efficient antibacterial and bactericidal effects, but also takes into account damaged tissue repair, inflammation relief and comfort of use, and achieves effective cleaning, health care and hygienic care for genital discomfort caused by Staphylococcus aureus, Candida albicans and Escherichia coli. DETAILED DESCRIPTION

[0068] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions in the embodiments of the present invention will be further clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0069] Example 1:

[0070] The present invention provides a technical solution: a silver ion polypeptide antibacterial gel composed of the following components: 0.5% silver ions, 2.5% polypeptides, 75% deionized water, 3% Thuja chinensis leaf extract, 3.5% Portulaca oleracea extract, 2.5% Panax notoginseng root extract, 4.5% Sophora flavescens extract, 3% Saffron extract, 3.5% Apple seed extract, 2.5% Amurense extract, 4.5% Cnidium monnieri extract, 3% Sunflower extract, 3.5% Centella asiatica extract, and 1.8% Peppermint extract.

[0071] A method for preparing a silver ion polypeptide antibacterial gel comprises the following steps:

[0072] S1: Extraction Preparation:

[0073] Thuja chinensis leaves, Portulaca oleracea, Panax notoginseng root, Sophora flavescens, Crocus sativus, Apple seed, Ligusticum chuanxiong, Cnidium monnieri, Helianthus annuus, Centella asiatica, and Mentha chinensis were pretreated to remove impurities. The raw materials were then added to an ethanol-water mixture (50% ethanol by volume) at a material-to-liquid ratio of 1:10 (g / mL) and then added to an extraction vessel. Ultrasonic-assisted extraction was performed using an ultrasonic power of 400 W and a temperature of 50°C for two extractions of 1.5 hours each. The combined extracts were concentrated in a rotary evaporator at 60°C and -0.08 MPa under reduced pressure to obtain the plant extracts for later use.

[0074] S2: Silver ion preparation:

[0075] A 0.1 mol / L silver nitrate solution and a 0.2 mol / L sodium citrate solution were mixed in a volume ratio of 1:2 and stirred at 300 rpm at 60°C for 1.5 hours to produce a silver ion solution with uniform particle size and good dispersion. Transmission electron microscopy (TEM) analysis of the silver ion particle size revealed an average particle size between 20 and 30 nm.

[0076] S3: Mixing and stirring:

[0077] Add deionized water to a stainless steel reactor equipped with a thermostat and agitator. Turn on the agitator, adjust the stirring speed to 200 rpm, and control the temperature at 35°C. First, add the prepared plant extracts and stir for 15 minutes to fully dissolve them. Then, slowly add the silver ion solution and continue stirring for 20 minutes. Finally, add the polypeptide and continue stirring for 25 minutes to thoroughly mix all the ingredients.

[0078] S4: Gel preparation:

[0079] To the mixed solution, 1.5% carbomer was added, and the mixture was stirred at 300 rpm until a uniform gel was formed. The pH was adjusted to 6.2 using a 1 mol / L sodium hydroxide solution. The gel was transferred to a vacuum degassing apparatus and allowed to stand at -0.06 MPa for 30 minutes to defoam, yielding a silver ion polypeptide antibacterial gel.

[0080] Experimental Example 1:

[0081] Antibacterial experiment:

[0082] Using a suspension quantitative bactericidal test method, the silver ion polypeptide antibacterial gel of the present invention was prepared into solutions with different concentration gradients. The solutions were mixed with suspensions of Candida albicans, Staphylococcus aureus, and Escherichia coli, respectively. After incubation at 37°C for 20 minutes, samples were taken for viable bacteria count. The results showed that when the gel solution concentration was 10%, the inhibition rate against Candida albicans reached 99.8%, the inhibition rate against Staphylococcus aureus reached 99.6%, and the inhibition rate against Escherichia coli reached 99.7%.

[0083] Experimental Example 2:

[0084] Skin irritation test:

[0085] Ten healthy adult rabbits were selected and hair was removed from both sides of their backs, covering an area of ​​approximately 2 cm × 2 cm. The silver ion polypeptide antibacterial gel of the present invention was applied to one side, while normal saline was applied to the other side as a control. The gel was applied once daily and observed for 7 consecutive days. During the experiment, no irritation reactions such as erythema and edema occurred on the skin areas where the gel was applied, and the skin reaction scores were all 0, indicating that the gel was non-irritating to the skin.

[0086] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A silver ion polypeptide antibacterial gel, characterized in that: It is composed of the following ingredients: silver ion 0.1-1%, polypeptide 0.5-3%, deionized water 60-85%, Thuja chinensis leaf extract 1-5%, Portulaca oleracea extract 1-5%, Panax notoginseng root extract 1-5%, Sophora flavescens extract 1-5%, Saffron extract 1-5%, Apple seed extract 1-5%, Atractylodes lancea extract 1-5%, Cnidium monnieri extract 1-5%, Sunflower extract 1-5%, Centella asiatica extract 1-5%, and peppermint extract 0.5-3%.

2. The silver ion polypeptide antibacterial gel according to claim 1, characterized in that: It is composed of the following ingredients: 0.1% silver ions, 3% polypeptide, 60% deionized water, 5% Thuja chinensis leaf extract, 1% Portulaca oleracea extract, 5% Panax notoginseng root extract, 1% Sophora flavescens extract, 5% Saffron extract, 1% Apple seed extract, 5% Atractylodes macrocephala extract, 1% Cnidium monnieri extract, 5% Sunflower extract, 1% Centella asiatica extract, and 3% Peppermint extract.

3. The silver ion polypeptide antibacterial gel according to claim 1, characterized in that: It is composed of the following ingredients: 1% silver ion, 0.5% polypeptide, 85% deionized water, 1% Thuja chinensis leaf extract, 5% Portulaca oleracea extract, 1% Panax notoginseng root extract, 5% Sophora flavescens extract, 1% Saffron extract, 5% Apple seed extract, 1% Atractylodes lancea extract, 5% Cnidium monnieri extract, 1% Sunflower extract, 5% Centella asiatica extract, and 0.5% Peppermint extract.

4. The silver ion polypeptide antibacterial gel according to claim 1, characterized in that: It is composed of the following ingredients: 0.5% silver ions, 2.5% polypeptide, 75% deionized water, 3% Thuja chinensis leaf extract, 3.5% Portulaca oleracea extract, 2.5% Panax notoginseng root extract, 4.5% Sophora flavescens extract, 3% Saffron extract, 3.5% Apple seed extract, 2.5% Atractylodes lancea extract, 4.5% Cnidium monnieri extract, 3% Sunflower extract, 3.5% Centella asiatica extract, and 1.8% Peppermint extract.

5. The method for preparing the silver ion polypeptide antibacterial gel according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1: Extraction Preparation: Thuja chinensis leaves, purslane, notoginseng root, sophora flavescens, saffron, apple seeds, radix ruticosae, cnidium monnieri, sunflower, centella asiatica, and mint were pretreated to remove impurities, and then extracted 2-3 times at 40-60° C. with an ethanol-water mixture as the extraction solvent, each extraction time for 1-2 hours, and the extracts were combined and concentrated under reduced pressure to obtain the plant extracts for later use; S2: Preparation of silver ions: Using a chemical reduction method, silver nitrate solution and a reducing agent are mixed in a certain proportion, and reacted at 50-70°C for 1-2 hours under stirring to obtain a silver ion solution; S3: Mixing and stirring: Add deionized water to the reactor, turn on the stirrer, control the temperature at 30-40°C, add the above-prepared plant extracts, silver ion solution and polypeptide in sequence, and continue stirring for 30-60 minutes to fully mix the ingredients; S4: Gel preparation: Add gel matrix to the above mixed solution, continue stirring until a uniform gel is formed, adjust the pH value to 5.5-7.0, let it stand for defoaming, and obtain silver ion polypeptide antibacterial gel.

6. The method for preparing the silver ion polypeptide antibacterial gel according to claim 5, wherein: The reducing agent in step S2 is sodium citrate solution, and the mixing ratio of the silver nitrate solution to the reducing agent is 1:1.5-2.

5.

7. The method for preparing the silver ion polypeptide antibacterial gel according to claim 5, wherein: The gel matrix in step S4 is carbomer.