Tau protein detection kit of double-antibody sandwich coupled DNAzyme and application of Tau protein detection kit

The Tau protein detection kit with double antibody sandwich coupled DNAzyme combines specific antibodies and DNAzyme components to achieve high-sensitivity and high-specificity Tau protein detection, which solves the shortcomings of existing methods and is suitable for early diagnosis and disease monitoring of Alzheimer's disease.

CN120594842AActive Publication Date: 2025-09-05CHINA MEDICAL BIOTECHNOLOGY (BEIJING) CO LTD
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Patent Information

Application Number
CN202510745415.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-05
Publication Date
2025-09-05
Estimated Expiration
2045-06-05

AI Technical Summary

Technical Problem

Existing Tau protein detection methods are cumbersome to operate, lack sensitivity and specificity, and are unable to meet the needs of early diagnosis of Alzheimer's disease.

Method used

The Tau protein detection kit uses a double antibody sandwich coupled to DNAzyme, which combines specific antibodies with DNAzyme components and achieves efficient signal conversion through fluorescent probes. Combined with simple operation steps and a suitable buffer environment, it achieves highly sensitive and specific detection.

Benefits of technology

It achieves sensitive detection of low-concentration Tau protein with a detection limit of 6.0 pg/mL, simplifies the detection process, reduces sample volume and time requirements, and is suitable for early diagnosis and large-scale screening.

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Abstract

The invention relates to a Tau protein detection kit with double antibody sandwich coupled DNAzyme and application thereof, and relates to the technical field of biological detection, the Tau protein detection kit comprises a first antibody coupled with a first constituent part of DNAzyme with catalytic activity, a second antibody coupled with a second constituent part of DNAzyme with catalytic activity, a fluorescent probe and a reaction buffer solution. The detection method provided by the invention can accurately capture the tiny signal of Tau protein, and provides powerful support for early diagnosis of neurodegenerative diseases such as Alzheimer's disease and the like.
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Citation Information

Patent Citations

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