Nanometer iron oxide and donkey-hide gelatin synergistic blood replenishing nutrition formula and preparation method thereof

Abstract

The invention discloses a nano iron oxide and colla corii asini synergistic blood replenishing nutrition formula and a preparation method thereof. The formula comprises the following components: 0.5-3% of surface functionalized nano iron oxide, 0.5-3% of colla corii asini and the balance of water. 30-50% of a donkey-hide gelatin matrix; 0.5 to 2.5% of vitamin C or a vitamin C derivative; 2-7% of plant polysaccharide; 0.5-3% of a licorice extract; 40-55% of ice syrup; the preparation method comprises the following steps: dissolving the donkey-hide gelatin matrix, adding the premixed nano iron oxide and vitamin C derivative, carrying out ultrasonic dispersion, sequentially adding other components, and carrying out homogenization treatment. According to the invention, sodium citrate or gelatin-coated nano iron oxide is used as a high-efficiency iron source and is matched with the blood replenishing effect of donkey-hide gelatin to realize function synergy; the bioavailability of iron is remarkably improved through the compatibility of vitamin C and polysaccharide; the nutritional powder has the advantages of traditional nourishing and modern nutritional supplement, and can be prepared into various dosage forms such as paste and soft sweets. In addition, the in-vitro absorption rate is 1.8-2.5 times of that of a traditional iron agent, the gastrointestinal irritation is remarkably reduced, and the iron-containing compound preparation is particularly suitable for nutrition intervention of people suffering from iron-deficiency anemia.

Description

Technical Field

[0001] The present invention relates to the technical field of food and nanomaterials, and in particular to a nutritional formula for synergistically enriching blood with nano iron oxide and donkey-hide gelatin and a preparation method thereof. Background Art

[0002] Donkey-hide gelatin, a traditional tonic, has benefits such as nourishing blood, nourishing yin and moistening dryness. However, its low iron content (typically less than 0.1%) makes it difficult to rapidly replenish the iron needed by patients with iron-deficiency anemia. While donkey-hide gelatin can promote hematopoiesis, its iron-replenishing efficiency is limited when used alone, making it unsuitable as an effective iron supplement.

[0003] Although the iron supplements currently available on the market (such as ferrous sulfate, ferrous fumarate, etc.) have a high iron content, they have obvious disadvantages: gastrointestinal irritation: inorganic iron supplements can easily cause adverse reactions such as nausea, abdominal pain, and constipation, and patient compliance is poor; poor taste: the unique metallic smell of iron supplements affects the taking experience, especially for children and pregnant women; low absorption rate: affected by food components (such as phytic acid and tannic acid), the bioavailability of traditional iron supplements is usually less than 10%.

[0004] Nano-iron oxide (e.g., Fe₃O₄) is considered a candidate for a new generation of iron supplements due to its high surface area and controlled release properties. However, existing technologies still face the following challenges: insufficient stability: nanoparticles tend to aggregate, affecting their dispersion and absorption in the gastrointestinal tract; lack of synergistic formulations: no mature technical solutions have been found for combining with traditional blood-tonifying herbs such as donkey-hide gelatin, making it difficult to achieve a synergistic "blood-tonifying and iron-tonifying" effect; taste and safety: unmodified nano-iron may affect product palatability, and long-term biosafety requires further verification.

[0005] Currently, there are no publicly available technical solutions for the synergistic use of surface-functionalized nano-iron oxide and donkey-hide gelatin for nutritional intervention. The simple combination of traditional iron supplements and donkey-hide gelatin cannot address issues such as low absorption and high irritation. Furthermore, there is still a lack of systematic research on the optimized combination of nano-iron and donkey-hide gelatin, particularly in terms of dosage form compatibility (such as ointments, soft candies, etc.) and functional synergy (blood-tonifying and fatigue-fighting effects).

[0006] Therefore, in response to the above problems, the present invention provides a nutritional formula for synergistic blood-enriching by nano-iron oxide and donkey-hide gelatin and a preparation method thereof. The present invention aims to develop a new nutritional formula, which achieves synergistic functions such as blood-enriching and anti-fatigue on the basis of ensuring safety, taste and absorption rate through the synergistic combination of surface functionalized nano-iron oxide and donkey-hide gelatin, combined with auxiliary materials such as vitamin C and plant polysaccharides, and is suitable for nutritional health care applications. Summary of the Invention

[0007] The purpose of the present invention is to provide a nutritional formula for synergistically replenishing blood by nano-iron oxide and donkey-hide gelatin and a preparation method thereof, so as to achieve synergistic functions such as blood replenishing and anti-fatigue, and is suitable for nutritional health care applications.

[0008] The purpose of the present invention is achieved through the following technical solutions:

[0009] A nutritional formula for synergistic blood-tonifying effect of nano-iron oxide and donkey-hide gelatin, comprising the following components by mass percentage:

[0010] Surface functionalized nano-iron oxide 0.5-3wt%;

[0011] Donkey-hide gelatin matrix 30-50wt%;

[0012] 0.5-2.5wt% of vitamin C or vitamin C derivative;

[0013] Plant polysaccharides 2-7wt%;

[0014] Licorice extract 0.5-3wt%;

[0015] Rock sugar syrup 40-55wt%.

[0016] Preferably, the surface functionalized nano-iron oxide is Fe3O4 nanoparticles coated with sodium citrate or gelatin, with an average particle size of 2 to 8 nm and a specific surface area of ​​150 m 2 / g;

[0017] The donkey-hide gelatin matrix is ​​donkey hide gelatin hydrolyzate with a molecular weight of 1 to 50 kDa.

[0018] Preferably, the plant polysaccharide is red date polysaccharide or wolfberry polysaccharide.

[0019] Preferably, the glycyrrhizic acid content in the licorice extract is ≥10%;

[0020] The sugar content of the rock sugar syrup is 65-75°Brix.

[0021] Preferably, the vitamin C derivative is selected from one of sodium ascorbate, calcium ascorbate, and ascorbyl palmitate.

[0022] This application also claims a method for preparing the above-mentioned nutritional formula of nano-iron oxide and donkey-hide gelatin for synergistic blood enrichment, comprising the following steps:

[0023] (1) Dissolve the donkey-hide gelatin matrix in a water bath at 50-60°C to form a gelatin solution;

[0024] (2) premixing the surface functionalized nano-iron oxide with vitamin C or a vitamin C derivative, and then adding the mixture to the gel to disperse it by ultrasonication;

[0025] (3) adding plant polysaccharide, licorice extract and rock sugar syrup in sequence and homogenizing;

[0026] (4) Pour the homogenized material into a mold and cool it into a block of donkey-hide gelatin cake, which is then packaged and stored.

[0027] Preferably, in step (2), the ultrasonic dispersion is carried out under nitrogen protection, the ultrasonic dispersion power is 200 to 400 W, and the ultrasonic dispersion time is 15 to 30 min;

[0028] In step (3), the homogenization speed is 2000-5000 rpm, the temperature is controlled at 45-55° C., and the homogenization time is 10-20 min.

[0029] The present application also claims the use of the above-mentioned nutritional formula of nano-iron oxide and donkey-hide gelatin for synergistic blood-enrichment in the preparation of an iron-enriching functional food, wherein the functional food is in the form of an ointment, chewable soft candy, oral liquid or instant powder;

[0030] The daily intake provides 3-12 mg of iron and 1.5-4 g of active ingredients in donkey-hide gelatin.

[0031] Preferably, the functional food has both the functions of improving iron deficiency anemia and resisting fatigue; wherein the bioavailability of nano-iron oxide is 1.5 to 2.2 times that of ferrous sulfate.

[0032] The working mechanism of the invention is as follows: the nourishing effect of donkey-hide gelatin is used as a base, and nano-iron oxide is used in combination as an iron source; sodium citrate or gelatin is coated on the surface of the nano-iron oxide to improve its dispersibility and biocompatibility; vitamin C and polysaccharides are combined to improve the stability and absorption of iron ions; the composition can be made into ointments, soft candies, powders, oral liquids, etc.

[0033] Due to the application of the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0034] 1. The present invention significantly improves iron absorption efficiency. It uses surface-functionalized nano-iron oxide as an iron source. Its small particle size (2-10 nm) and large specific surface area make its intestinal permeability significantly higher than that of traditional iron supplements (such as ferrous sulfate). Experiments have shown that the bioavailability of iron in this formula is 1.8-2.5 times that of traditional iron supplements, which can more quickly improve the symptoms of iron deficiency anemia.

[0035] 2. The present invention is safe, mild, and has good palatability. By coating nano-iron oxide with sodium citrate or gelatin, the free degree of iron ions is effectively reduced, reducing irritation to the gastrointestinal mucosa and avoiding the common side effects of traditional iron supplements such as nausea and constipation. At the same time, natural flavoring ingredients such as rock sugar syrup and licorice extract are added to the formula to mask the iron smell and improve the taste, making the product more acceptable to sensitive groups such as children and pregnant women.

[0036] 3. The present invention synergistically enhances blood-tonifying and nutritional benefits. Donkey-hide gelatin provides small molecule peptides and amino acids to improve the hematopoietic microenvironment; vitamin C enhances iron absorption and conversion while exerting an antioxidant effect; red date polysaccharides and wolfberry polysaccharides further regulate immune function and synergistically enhance red blood cell production efficiency. This combination not only replenishes iron quickly but also improves comprehensive symptoms such as fatigue and weakness caused by insufficient Qi and blood.

[0037] 4. The present invention has diverse dosage forms and a wide range of application scenarios. The formula can be adapted to various dosage forms according to needs: traditional paste prescriptions (such as donkey-hide gelatin cakes): retain the nourishing characteristics and are suitable for tonic in autumn and winter; modern dosage forms (such as soft candies and oral liquids): are easy to carry and for daily supplementation; functional decoction pieces: can be combined with other nourishing ingredients to expand into niche markets such as women's health and postoperative rehabilitation;

[0038] 5. The industrialization advantages of this invention are outstanding. Raw materials are easily available: donkey-hide gelatin, nano-iron oxide, vitamin C, etc. are all mature raw materials with a stable supply chain; the process compatibility is strong: conventional mixing, homogenization, and molding technologies are adopted, and no complex equipment is required, making it suitable for large-scale production; the cost is controllable: the amount of nano-iron oxide used is low, which reduces the cost of raw materials while ensuring the effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for use in the specific embodiments or the description of the prior art. Obviously, some of the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be made based on these drawings without paying any creative work.

[0040] Figure 1 Schematic diagram of the working principle of each component in the formula of Example 1 of the present invention. DETAILED DESCRIPTION

[0041] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, a specific implementation scheme is now described in detail.

[0042] The present invention is further described below with reference to the following examples, but the present invention is not limited to the following examples. The implementation conditions used in the examples can be further adjusted according to the different requirements of specific applications. The implementation conditions not specified are conventional conditions in the industry. The technical features involved in the various embodiments of the present invention may be combined with each other as long as they do not conflict with each other.

[0043] Example 1

[0044] See attached Figure 1 This embodiment provides a nutritional formula for synergistic blood-tonifying effect of nano-iron oxide and donkey-hide gelatin, which includes the following components by mass percentage:

[0045] Surface functionalized nano-iron oxide 2wt%;

[0046] Donkey-hide gelatin solution 40wt%;

[0047] Sodium ascorbate 1wt%;

[0048] Red date polysaccharide 5wt%;

[0049] Licorice extract 2wt%;

[0050] Rock sugar syrup 50wt%.

[0051] The surface functionalized nano-iron oxide is Fe3O4 nanoparticles coated with sodium citrate or gelatin, with an average particle size of 4.5nm and a specific surface area of ​​≥150m 2 / g;

[0052] The donkey-hide gelatin matrix is ​​donkey hide gelatin hydrolyzate with a molecular weight of 25 kDa;

[0053] The glycyrrhizic acid content in the licorice extract is ≥10%;

[0054] The sugar content of the icing syrup is 70°Brix.

[0055] The preparation method of the above-mentioned nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin comprises the following steps:

[0056] (1) Dissolve the donkey-hide gelatin matrix in a 50°C water bath to form a gelatin solution;

[0057] (2) After premixing the surface functionalized nano-iron oxide and sodium ascorbate, the mixture was added to the glue solution and ultrasonically dispersed under nitrogen protection at a power of 300 W for 20 min.

[0058] (3) adding red date polysaccharide, licorice extract and rock sugar syrup in sequence, homogenizing at a speed of 3000 rpm, controlling the temperature at 50°C, and homogenizing for 20 minutes;

[0059] (4) Pour the homogenized material into a mold and cool it into a block of donkey-hide gelatin cake, which is then packaged and stored.

[0060] The use of the above-mentioned nutritional formula of nano-iron oxide and donkey-hide gelatin for synergistic blood-enrichment in the preparation of iron-enriching functional food, wherein the functional food is in the form of an ointment, chewable candy, oral liquid or instant powder;

[0061] The daily intake provides 8 mg of iron and 2 g of active ingredients of donkey-hide gelatin; the functional food also has the functions of improving iron deficiency anemia and resisting fatigue.

[0062] Comparative Example 1

[0063] This comparative example is carried out on the basis of the above-mentioned Example 1, and the similarities with Example 1 are not repeated here.

[0064] In this comparative example, it is FeSO4.

[0065] Comparative Example 2

[0066] This comparative example is carried out on the basis of the above-mentioned Example 1, and the similarities with Example 1 are not repeated here.

[0067] This comparative ratio was physically mixed.

[0068] The products of Example 1, Comparative Example 1 and Comparative Example 2 were subjected to biological activity tests, and the iron permeability was tested in an in vitro Caco-2 cell model. The test results are shown in Table 1.

[0069] Table 1

[0070] Test items Iron permeability Example 1 180％ Comparative Example 1 100％ Comparative Example 2 90％

[0071] The products of Example 1, Comparative Example 1, and Comparative Example 2 were tested for iron release rate in simulated gastric fluid. The test results are shown in Table 2.

[0072] Table 2

[0073]

[0074] The products of Example 1, Comparative Example 1, and Comparative Example 2 were tested for iron release rate in simulated intestinal fluid. The test results are shown in Table 3.

[0075] Table 3

[0076]

[0077] As can be seen from the above table, in the in vitro Caco-2 cell model, the iron permeability is 1.8 times that of traditional FeSO4; the present invention shows significant advantages in iron ion release characteristics in a simulated gastrointestinal environment. Through scientific proportions and nanotechnology modification, it achieves the ideal release pattern of "stomach protection and intestinal efficiency". In the simulated gastric fluid (pH 1.2) test, the sodium citrate-coated nano-iron oxide of the present invention showed a moderate release rate, effectively avoiding the mucosal irritation problem caused by the premature and large-scale release of traditional iron supplements in the stomach, while preventing the iron ions from reacting with gastric acid to form insoluble precipitates. When entering the simulated intestinal fluid (pH 6.8) environment, the iron ion release rate increases rapidly under the synergistic effect of the active ingredients of donkey-hide gelatin and vitamin C, significantly exceeding the release level of traditional ferrous sulfate. This intelligent phased release characteristic not only ensures the efficient release of iron in the main absorption site (small intestine), but also reduces gastrointestinal adverse reactions through surface functionalization, making the product's bioavailability 1.8 to 2.5 times that of traditional iron supplements, perfectly balancing the contradiction between iron supplement efficiency and comfort.

[0078] In summary, the iron absorption efficiency of the present invention is significantly improved. By using surface-functionalized nano-iron oxide as the iron source, it has a small particle size (2 - 10 nm) and a large specific surface area, and its transmittance in the intestine is significantly higher than that of traditional iron agents (such as ferrous sulfate); experiments show that the bioavailability of iron in this formulation reaches 1.8 - 2.5 times that of traditional iron agents and can improve iron deficiency anemia symptoms more quickly; the present invention is safe, mild, and has good palatability. By coating nano-iron oxide with sodium citrate or gelatin, the free degree of iron ions is effectively reduced, the irritation to the gastrointestinal mucosa is reduced, and side effects common to traditional iron agents such as nausea and constipation are avoided; at the same time, natural flavoring components such as rock sugar syrup and licorice extract are added to the formulation to cover the iron smell and improve the taste, making the product more acceptable to sensitive groups such as children and pregnant women; the present invention synergistically enhances the effects of blood tonifying and nutrition. Ejiao provides small molecular peptides and amino acids to promote the improvement of the hematopoietic microenvironment; vitamin C enhances the absorption and conversion of iron and simultaneously plays an antioxidant role; jujube polysaccharide and wolfberry polysaccharide further regulate the immune function and synergistically improve the efficiency of red blood cell production; this combination not only has a fast iron supplement speed but also can improve comprehensive symptoms such as fatigue and weakness caused by qi and blood deficiency; the present invention has various dosage forms and wide application scenarios. The formulation can be adapted to a variety of dosage forms according to needs: traditional paste formulas (such as Ejiao cakes): retaining the nourishing characteristics and suitable for tonic supplementation in autumn and winter; modern dosage forms (such as soft candies, oral liquids): convenient for carrying and daily supplementation; functional cut pieces: can be combined with other nourishing components and extended to segmented markets such as women's health and postoperative rehabilitation; the present invention has prominent industrialization advantages. The raw materials are easily available: Ejiao, nano-iron oxide, vitamin C, etc. are all mature raw materials with a stable supply chain; the process has strong compatibility: using conventional mixing, homogenization, and forming technologies, without complex equipment and suitable for large-scale production; the cost is controllable: the dosage of nano-iron oxide is low, reducing the raw material cost while ensuring the effect.

[0079] The above-described embodiments only represent one implementation mode of the present invention, and its description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. A nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin, characterized in that: Calculated by mass percentage, it includes the following components: Surface functionalized nano-iron oxide 0.5-3wt%; Donkey-hide gelatin matrix 30-50wt%; 0.5-2.5wt% of vitamin C or vitamin C derivative; Plant polysaccharides 2-7wt%; Licorice extract 0.5-3wt%; Rock sugar syrup 40-55wt%.

2. The nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin according to claim 1, characterized in that: The surface functionalized nano-iron oxide is Fe3O4 nanoparticles coated with sodium citrate or gelatin, with an average particle size of 2 to 8 nm and a specific surface area of ​​150 m 2 / g; The donkey-hide gelatin matrix is ​​donkey hide gelatin hydrolyzate with a molecular weight of 1 to 50 kDa.

3. The nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin according to claim 1, characterized in that: The plant polysaccharide is red date polysaccharide or wolfberry polysaccharide.

4. The nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin according to claim 1, characterized in that: The glycyrrhizic acid content in the licorice extract is ≥10%; The sugar content of the rock sugar syrup is 65-75°Brix.

5. The nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin according to claim 1, characterized in that: The vitamin C derivative is selected from one of sodium ascorbate, calcium ascorbate and ascorbyl palmitate.

6. A method for preparing the nutritional formula of nano-iron oxide and donkey-hide gelatin for synergistic blood enrichment as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: (1) Dissolve the donkey-hide gelatin matrix in a water bath at 50-60°C to form a gelatin solution; (2) premixing the surface functionalized nano-iron oxide with vitamin C or a vitamin C derivative, and then adding the mixture to the gel to disperse it by ultrasonication; (3) adding plant polysaccharide, licorice extract and rock sugar syrup in sequence and homogenizing; (4) Pour the homogenized material into a mold and cool it into a block of donkey-hide gelatin cake, which is then packaged and stored.

7. The method for preparing the nutritional formula for synergistic blood-enriching with nano-iron oxide and donkey-hide gelatin according to claim 6, characterized in that: In step (2), the ultrasonic dispersion is carried out under nitrogen protection, the ultrasonic dispersion power is 200-400W, and the ultrasonic dispersion time is 15-30min; In step (3), the homogenization speed is 2000-5000 rpm, the temperature is controlled at 45-55° C., and the homogenization time is 10-20 min.

8. Use of the nutritional formula of nano-iron oxide and donkey-hide gelatin for synergistic blood-enrichment according to any one of claims 1 to 5 in the preparation of iron-enriched functional foods, characterized in that: The functional food is in the form of a paste, chewable candy, oral liquid or instant powder; The daily intake provides 3-12 mg of iron and 1.5-4 g of active ingredients in donkey-hide gelatin.

9. The use of the nutritional formula of nano-iron oxide and donkey-hide gelatin for synergistic blood-enrichment according to claim 8 in the preparation of iron-enriched functional food, characterized in that: The functional food has the functions of improving iron deficiency anemia and resisting fatigue; the bioavailability of nano-iron oxide is 1.5 to 2.2 times that of ferrous sulfate.

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