Oil control composition, application and skin care product

Abstract

The invention belongs to the technical field of skin care, and discloses an oil control composition, application and a skincare product, and the oil control composition comprises the following components in parts by weight: 0.5-1 part of PCA zinc, 4-9 parts of nicotinamide, 0.5-2 parts of pink passion flower extract and 0.3-0.7 part of acetyl tetrapeptide-5. The oil control composition not only can inhibit the activity of 5 alpha-reductase, but also has the effect of promoting sebaceous gland cell autophagy, so that the oil control effect of the composition is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, and in particular to an oil control composition, uses thereof and a skin care product. Background Art

[0002] The skin contains numerous sebaceous glands, which secrete oil through the sebaceous ducts of the hair follicles to the skin's surface, moisturizing the skin and helping to maintain a slightly acidic environment on the skin's surface. Under normal circumstances, sebum secreted by the sebaceous glands maintains skin moisture and integrity. However, with increasing environmental pollution, stressful lifestyles and work, unhealthy habits like staying up late, and puberty, sebum secretion increases abnormally. Excessive oil not only makes the skin look greasy but also easily clogs pores, leading to skin problems such as acne, blackheads, and blackheads.

[0003] Currently, most skin care products solve the problem of excessive skin oil by inhibiting 5α-reductase. For example, prior art 1: Chinese patent application 202110915319.7 discloses a balanced oil composition and oil control gel that inhibits 5α-reductase activity, including a solvent, a thickener, a moisturizer, a preservative, a putrefactive agent, a pH regulator, and a balanced oil composition. The preparation method of the preparation comprises the following steps: S1: Accurately weigh the components in the formula and clean the production equipment at the same time; S2: Add the solvent, moisturizer, and thickener to the emulsifier, stir and heat to 85-90°C, turn on the homogenizer and homogenize for 3-5 minutes, dissolve completely, and sterilize at a constant temperature for 10 minutes; S3: Stir and cool to 70-75°C, add the preservative, stir for 3 minutes, and vacuum and keep the temperature to defoam completely; S4: Stir and cool to 40-45°C, add the pH regulator, excipient, and acne conditioning composition in turn, stir evenly, and the production is completed.

[0004] This patent application regulates excessive oil secretion of sebaceous glands by inhibiting the activity of 5α-reductase, inhibits the activity of 5α-reductase, regulates oil secretion, and achieves water-oil balance.

[0005] 5α-reductase is a membrane protease that depends on reduced coenzyme II (NADPH). It is an important male hormone metabolic enzyme in the skin. It can irreversibly convert testosterone into dihydrotestosterone (DHT). DHT can induce excessive oil secretion of sebaceous glands. By inhibiting the activity of 5α-reductase to reduce the level of DHT, it can effectively relieve excessive oil secretion of sebaceous glands.

[0006] Currently, many existing technologies achieve oil control effects by inhibiting 5α-reductase, and rarely alleviate the problem of excessive sebum secretion from sebaceous glands through other directions; achieving excellent oil control effects in a single direction requires a high content of active ingredients, which is costly and prone to skin safety issues. Summary of the Invention

[0007] One of the objectives of the present invention is to provide an oil-control composition that regulates sebum secretion from sebaceous glands by inhibiting 5α-reductase activity and promoting autophagy in sebaceous gland cells, thereby effectively improving the oil-control effect of the composition and effectively avoiding the cost and skin safety issues caused by achieving oil-control efficacy in a single direction.

[0008] Another object of the present invention is to provide a use of an oil-control composition, which can be used to prepare skin care products to effectively avoid the problem of excessive sebum secretion by sebaceous glands, thereby reducing pore blockage and the induction of skin problems such as acne, blackheads, and blackheads.

[0009] Another object of the present invention is to provide a skin care product containing the oil control composition of the present invention, which can control oil in multiple directions and bring more oil control options to the market.

[0010] In order to achieve the above objectives, the present invention provides an oil control composition, which comprises, by weight, 0.5-1 parts of PCA zinc, 4-9 parts of niacinamide, 0.5-2 parts of pink passionflower extract and 0.3-0.7 parts of acetyl tetrapeptide-5.

[0011] PCA zinc not only has the antibacterial, anti-sebum and anti-inflammatory effects of zinc, but also has the function of keeping the stratum corneum hydrated and maintaining a healthy barrier. Niacinamide is a derivative of vitamin B3 that can accelerate metabolism, promote the shedding of melanin-containing keratinocytes, promote the synthesis of epidermal proteins, and improve skin texture. It can also reduce the production of fatty acids and triglycerides in sebum, thereby shrinking pores and reducing the severity of acne. Pink passionflower extract mainly contains flavonoids, alkaloids and other substances, which have antioxidant, anti-inflammatory and nervous tension-relieving effects; Acetyl tetrapeptide-5 mainly regulates sebaceous gland cell differentiation by targeting the MC5R receptor of sebaceous cells to reduce sebum secretion.

[0012] The present invention combines four components: PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5, which not only effectively inhibits the activity of 5α-reductase, but also promotes autophagy of sebaceous gland cells. These two directions synergistically regulate the sebum secretion of the sebaceous glands to achieve excellent oil control effects.

[0013] The present invention provides use of an oil-control composition in preparing skin care products.

[0014] At the same time, the present invention provides a skin care product containing 1-3 wt % of the above-mentioned oil control composition.

[0015] Furthermore, the skin care products include lotions, creams, essences, and facial masks.

[0016] Beneficial effects

[0017] Compared with the prior art, the present invention has at least the following advantages: (1) The present invention discloses an oil control composition, which produces a significant effect of inhibiting 5α-reductase activity by compounding four components: PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5; (2) The oil-control composition of the present invention can not only inhibit the activity of 5α-reductase, but also promote the autophagy of sebaceous gland cells, thereby enhancing the oil-control efficacy of the composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described below with reference to the accompanying drawings and embodiments; Figure 1 This is a test diagram of the chicken embryo chorioallantoic membrane vascular test in the application example of this application. DETAILED DESCRIPTION

[0019] The present invention will be further described below in conjunction with the embodiments, but this does not constitute any limitation to the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0020] In order to explain the technical content of the present invention in detail, further description will be given below in conjunction with the embodiments.

[0021] In the following examples and comparative examples, the PCA zinc was purchased from Guangzhou Aoxue Chemical Co., Ltd.; the niacinamide was purchased from Guangzhou Hemiao Biotechnology Co., Ltd.; the pink passionflower extract was purchased from Guangzhou Miyanxiu Biotechnology Co., Ltd.; and the acetyl tetrapeptide-5 was purchased from Guangzhou Best Chemical Co., Ltd.

[0022] It should be noted that the technical solution of the present invention is not limited to the above raw materials, and all the same raw materials available on the market can realize the technical solution of the present invention.

[0023] Unless otherwise specified, in the following examples and comparative examples, the parts and % refer to parts by weight and percentage by weight, respectively. In the following examples and comparative examples, deionized water is used as a solvent, which does not affect the efficacy of the composition.

[0024] Example 1 An oil control composition is prepared by mixing 1 part of PCA zinc, 4 parts of niacinamide, 1.2 parts of pink passionflower extract, 0.5 parts of acetyl tetrapeptide-5 and 93.3 parts of deionized water.

[0025] Example 2 An oil control composition is prepared by mixing 0.5 parts of PCA zinc, 6 parts of niacinamide, 2 parts of pink passionflower extract, 0.3 parts of acetyl tetrapeptide-5 and 91.2 parts of deionized water.

[0026] Example 3 An oil control composition is prepared by mixing 0.8 parts of PCA zinc, 9 parts of niacinamide, 0.5 parts of pink passionflower extract, 0.7 parts of acetyl tetrapeptide-5 and 89 parts of deionized water.

[0027] Comparative Example 1 An oil-control composition is prepared by mixing 6.3 parts of niacinamide, 2.1 parts of pink passionflower extract, 0.4 parts of acetyl tetrapeptide-5 and 91.2 parts of deionized water.

[0028] Comparative Example 2 An oil control composition is prepared by mixing 1.5 parts of PCA zinc, 6.3 parts of pink passionflower extract, 1 part of acetyl tetrapeptide-5 and 91.2 parts of deionized water.

[0029] Comparative Example 3 An oil control composition is prepared by mixing 0.6 parts of PCA zinc, 7.8 parts of niacinamide, 0.4 parts of acetyl tetrapeptide-5 and 91.2 parts of deionized water.

[0030] Comparative Example 4 An oil-control composition is prepared by mixing 0.5 parts of PCA zinc, 6.2 parts of niacinamide, 2.1 parts of pink passionflower extract and 91.2 parts of deionized water.

[0031] Comparative Example 5 An oil control composition is prepared by mixing 8.8 parts of PCA zinc and 91.2 parts of deionized water.

[0032] Comparative Example 6 An oil control composition is prepared by mixing 8.8 parts of niacinamide and 91.2 parts of deionized water.

[0033] Comparative Example 7 An oil-control composition is prepared by mixing 8.8 parts of pink passionflower extract and 91.2 parts of deionized water.

[0034] Comparative Example 8 An oil control composition is prepared by mixing 8.8 parts of acetyl tetrapeptide-5 and 91.2 parts of deionized water.

[0035] Efficacy testing 1. 5α-reductase activity inhibition rate 5α-reductase is a key enzyme that catalyzes the conversion of testosterone to dihydrotestosterone, which stimulates excessive oil secretion in the sebaceous glands. The 5α-reductase inhibition test indirectly assesses the oil-control ability of the test substance by detecting its inhibitory effect on enzyme activity. During the experiment, 5α-reductase metabolizes testosterone into dihydrotestosterone, which then continues to react under the action of the enzyme. The product generated during this reaction has a specific peak at a wavelength of 405nm. By measuring the change in the absorbance of this product at a wavelength of 405nm, it is used to reflect the amount of dihydrotestosterone produced, and ultimately reflect the change in 5α-reductase activity, thereby evaluating the inhibitory effect of the sample.

[0036] Inhibition rate = (control group - sample group) / (control group - blank group) * 100%; The control group was a base without the composition; The blank group contained no composition and base material; The sample groups are Examples 1-3 and Comparative Examples 1-4.

[0037] The inhibitory effects of Examples 1-3 and Comparative Examples 1-4 are shown in Table 1; Table 1 Test results of 5α-reductase activity inhibition rate of the compositions of Examples 1-3 and Comparative Examples 1-4 Absorbance Inhibition rate control group 0.996 - Blank group 0.051 - Example 1 0.251 78.84% Example 2 0.317 71.85% Example 3 0.386 64.55% Comparative Example 1 0.796 21.16% Comparative Example 2 0.489 53.65% Comparative Example 3 0.556 46.56% Comparative Example 4 0.678 33.65%

[0038] According to the results in Table 1, we can see that: According to the data results of Examples 1-3, the present application has an extremely high 5α-reductase activity inhibition rate through the technical solution of compounding PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5.

[0039] According to the comparison of the data of Example 2 and Comparative Example 1, when PCA zinc is missing and the dosage of other components is increased accordingly, the inhibition rate of 5α-reductase activity of the composition is significantly reduced. It is speculated that the reason may be: (1) PCA zinc itself has a very high 5α-reductase activity inhibition rate, while the other components themselves have a low 5α-reductase activity inhibition rate; (2) PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5 produced a synergistic effect. The lack of PCA zinc resulted in the disappearance or weakening of the synergistic inhibition of 5α-reductase activity.

[0040] However, according to the comparison of the data of Example 2 and Example 3, it can be seen that the content of PCA zinc in Example 3 is higher than that in Example 2, but the 5α-reductase activity inhibition rate produced is lower than that in Example 2, indicating that PCA zinc itself does not have the 5α-reductase activity inhibition efficacy that can cause the 5α-reductase activity inhibition rate of the technical solution of Comparative Example 1 to decrease by 50.69% compared with that of Example 2; therefore, the reason for the significant decrease in the 5α-reductase activity inhibition rate of Comparative Example 1 should be that: the combination of PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5 produces a synergistic effect, and the lack of PCA zinc causes the synergistic inhibition of 5α-reductase activity to disappear or be greatly weakened.

[0041] Similarly, according to the comparison of the data of Example 2 and Comparative Examples 2-4, the lack of any one of nicotinamide, pink passionflower extract and acetyl tetrapeptide-5 will also lead to a significant decrease in the 5α-reductase activity inhibition rate of the composition, further confirming that the combination of PCA zinc, nicotinamide, pink passionflower extract and acetyl tetrapeptide-5 has a synergistic effect of improving the 5α-reductase activity inhibition rate.

[0042] 2. Verification of Sebaceous Gland Cell Autophagy Autophagy is a self-protection mechanism prevalent in eukaryotic cells. Cells harbor aging and damaged organelles, missynthesized proteins, invading bacteria, and macromolecules. Autophagy encapsulates these harmful substances by forming autophagosomes, which then combine with lysosomes to form autolysosomes to break down and eliminate them, maintaining intracellular homeostasis and promoting the recycling of intracellular substances. Improving the autophagic capacity of sebaceous gland cells can increase the sebaceous gland's ability to metabolize sebum and reduce sebum secretion.

[0043] Western blotting was used to detect the expression of autophagy-related proteins LC3-Ⅱ and p62 in sebaceous gland cells to assess the level of autophagy. Upregulation of LC3-Ⅱ / β-actin and downregulation of p62 / β-actin indicate increased autophagy.

[0044] LC3-Ⅱ change rate = (sample group - blank group) / blank group * 100%; p62 change rate = (sample group - blank group) / blank group * 100%; The sample groups are Examples 1-3 and Comparative Examples 1-8; The blank group was a base material without adding the composition.

[0045] The sebaceous gland autophagy effects of Examples 1-3 and Comparative Examples 1-8 are shown in Table 2; Table 2 Sebaceous gland autophagy effects of the compositions of Examples 1-3 and Comparative Examples 1-8 LC3-Ⅱ / β-actin ratio LC3-Ⅱ change rate p62 / β-actin ratio p62 change rate Blank group 0.553 - 1.253 - Example 1 1.235 123.33% 0.635 -49.32% Example 2 0.989 78.84% 0.716 -42.86% Example 3 1.014 83.36% 0.683 -45.49% Comparative Example 1 0.627 13.38% 1.153 -7.98% Comparative Example 2 0.735 32.91% 0.983 -21.55% Comparative Example 3 0.639 15.55% 1.013 -19.15% Comparative Example 4 0.743 34.36% 0.939 -25.06% Comparative Example 5 0.697 26.04% 0.882 -29.61% Comparative Example 6 0.652 17.90% 1.113 -11.17% Comparative Example 7 0.686 24.05% 0.852 -32.00% Comparative Example 8 0.579 4.70% 1.208 -3.59% .

[0046] According to the data comparison in Table 2, we can see that: According to the data of Examples 1-3 of the present application, the technical solution of the present application has extremely high protein LC3-Ⅱ expression enhancement and p62 expression inhibition; and combined with the data comparison in Table 1, it can be seen that, unlike Table 1, the LC3-Ⅱ expression enhancement and p62 expression inhibition in Example 3 are higher than those in Example 2 but lower than those in Example 1. At the same time, the difference in LC3-Ⅱ change rate between Example 1 and Example 3 is much greater than the difference between Example 3 and Example 2, and the difference in p62 change rate between Example 1 and Example 3 is also greater than the difference between Example 3 and Example 2. It is speculated that the reason may be that PCA zinc plays an important role in enhancing LC3-Ⅱ and inhibiting p62 expression, but it is not the only important factor. The ratio of other components also plays an important role. Combined with the content of each component in Examples 1-3, it is likely that the combination of PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5 produces a synergistic effect of enhancing protein LC3-Ⅱ and inhibiting p62 expression.

[0047] According to the data of Comparative Examples 5-8, it can be seen that in the role of a single component in regulating the expression of LC3-Ⅱ and p62, PCA zinc > pink passion fruit extract > nicotinamide > acetyl tetrapeptide-5, and at the same dosage, PCA zinc and pink passion fruit extract have similar effects in promoting LC3-Ⅱ expression and inhibiting p62 expression, while the regulatory effect of acetyl tetrapeptide-5 on the expression of LC3-Ⅱ and p62 is not obvious.

[0048] According to the data of Comparative Examples 5-8, theoretically, Example 2 should have a better effect on regulating the expression of LC3-II and p62 than Example 1. However, in reality, the effect of Example 1 is significantly higher than that of Example 2. It is speculated that the reason why the actual effect is inconsistent with the expected effect may be: (1) In the synergistic combination of this composition, PCA zinc plays a major role in regulating the expression of LC3-Ⅱ and p62, and its dosage has a great influence on the efficacy of the composition; (2) Although acetyl tetrapeptide-5 itself does not have a significant regulatory effect on the expression of LC3-Ⅱ and p62, it plays an important synergistic role in the composition of the present application, so that the increase in its dosage seriously affects the efficacy of the composition.

[0049] However, combined with the data comparison of Example 1 and Example 3, the above two speculations have not been proven. It can be inferred that: in the technical solution of the present application, the combined dosage of PCA zinc, niacinamide, pink passionflower extract and acetyl tetrapeptide-5 is particularly important for promoting LC3-Ⅱ expression and inhibiting p62 expression. Under a specific ratio, the LC3-Ⅱ and p62 expression-regulating effect of the composition can be significantly enhanced.

[0050] Application Examples Configure the product according to Table 3. The specific configuration method is as follows: 1) Mix glycerol, carbomer, butylene glycol, macromolecular HA, and water, stir well, heat to 85°C, and keep warm for 15 minutes to obtain mixture A; 2) uniformly mixing the Olivem 1000, 1618 alcohol, jojoba oil, and squalane, heating to 85° C., and maintaining the temperature for 15 minutes to obtain a mixture B; 3) While mixture A is being stirred, add mixture B and mix until uniform; 4) Cool down to 40±2℃; add a small amount of water to dissolve the arginine and quinone, then add hexanediol, the composition, and the essence, and stir well.

[0051] Table 3 Product formula Components source Dosage% water - To 100 Cabo Guangzhou Zhaoheng Trading Development Co., Ltd. 0.2 glycerin Guangzhou Biono Biotechnology Co., Ltd. 5 Butanediol Guangzhou Hengtao Trading Co., Ltd. 2 Large molecule HA Guangzhou Hemiao Biotechnology Co., Ltd. 0.01 Olivem 1000 Guangzhou Biono Biotechnology Co., Ltd. 0.8 1618 alcohol Guangzhou Chichen Trading Co., Ltd. 0.3 Jojoba oil Guangzhou Baihaobo Co., Ltd. 2 Squalane Guangzhou Hengtao Trading Co., Ltd. 1 Arginine Guangzhou Hemiao Biotechnology Co., Ltd. 0.2 Composition Example 1 2 Xinxian Ketone Guangzhou Hemiao Biotechnology Co., Ltd. 0.4 Hexanediol Guangzhou Hemiao Biotechnology Co., Ltd. 0.5 essence Guangzhou Fangya Biotechnology Co., Ltd. 0.05

[0052] Application case test 3. Human Experiment Verification Test method: Referring to T / CAB 0152-2022 "Test Methods for Seven Efficacy of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing, and Soothing", 10 subjects were recruited to use the product on half of their faces. One side used a lotion without the added composition, and the other side used a lotion with the added composition. The sebum secretion was tested before and 14 days after use. On the day of the test, the subjects first used an alkaline soap-based cleanser to clean the test area. After cleaning, the skin oil secretion was measured at least 4 hours later. The test results are shown in Table 4: Table 4 Measurement results of skin oil secretion

[0053] Data showed that after using the lotion containing the combination, sebum secretion was significantly reduced (p<0.05) by 22.15% over the four hours following application, indicating that the combination has an oil-control effect.

[0054] 4. Mildness Test Test method: Refer to T / GDCA 036-2024 "Cosmetic eye irritation - Chicken chorioallantoic membrane vascular test method" to test the mildness of the application case. The test score is 1.33 points (stress stimulation). The test screenshot is as follows: Figure 1 : Figure 1 1-1, 1-2, and 1-3 represent three different chick embryo chorioallantoic membrane vascular experiments.

[0055] The embodiments presented herein are merely embodiments selected from a combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include subranges therein, and variations in these ranges should also be covered by the appended claims.

Claims

1. An oil control composition, characterized in that The invention comprises, by weight, 0.5-1 parts of PCA zinc, 4-9 parts of niacinamide, 0.5-2 parts of pink passionflower extract and 0.3-0.7 parts of acetyl tetrapeptide-5.

2. Use of the oil control composition according to claim 1 in preparing skin care products.

3. A skin care product, characterized in that: Contains 1-3 wt % of the oil control composition according to claim 1.

4. The skin care product according to claim 3, characterized in that The skin care products include lotions, creams, essences and facial masks.

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