A composition containing ultramicrovesicles, its preparation method, and its uses.

Abstract

This invention belongs to the field of cosmetic research technology, specifically relating to a composition containing ultramicrovesicles, its preparation method, and its uses. The composition comprises the following components by weight: 0.01–30.0 parts of animal umbilical cord extract, 0.001–5 parts of a mixture of pro-xylene and azelaic acid, and 0.1–10 parts of niacinamide; the mass ratio of pro-xylene to azelaic acid in the mixture is 2–3:2–3. This invention combines umbilical cord extract ultramicrovesicles, the pro-xylene and azelaic acid mixture, and niacinamide to achieve a synergistic effect, resulting in better and more comprehensive efficacy in three dimensions: anti-wrinkle and firming, whitening and brightening, and soothing and repairing after application to the skin.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic research technology, specifically relating to a composition containing ultramicrovesicles, its preparation method, and its uses. Background Technology

[0002] Skin is the largest organ in the human body, participating not only in vital physiological functions such as secretion and excretion, temperature regulation, immunity, respiration, and endocrine function, but also serving as a crucial symbol of beauty. Nowadays, with improved living standards and technology, people are paying increasing attention to skincare. As we age, and due to factors such as UV radiation, stress, lack of sleep, and excessive use of cosmetics, skin develops a series of problems, including wrinkles, dullness, and a damaged skin barrier. Good skin should be plump, firm, radiant, and have an intact skin barrier. Therefore, maintaining healthy skin requires not only a healthy lifestyle but also a skincare product that addresses wrinkles and firmness, brightening and whitening, and soothing and repairing.

[0003] The market is currently flooded with a growing number of products, which are becoming increasingly segmented. Most products target only a single skin concern; for example, anti-wrinkle and firming products primarily contain ingredients like pro-xylane, peptides, and retinol; whitening and brightening products mainly contain niacinamide, arbutin, and vitamin C; and soothing and repairing products mainly contain dipotassium glycyrrhizate, ceramides, and plant extracts. The drawback of these products is that they only address a specific skin problem and cannot maintain healthy skin. Furthermore, most products have a limited range of functionalities; low doses of a single active ingredient are ineffective, while excessive doses can cause irritation.

[0004] Therefore, it is essential to develop a product that can achieve good results in three dimensions: anti-wrinkle and firming, whitening and brightening, and soothing and repairing. Summary of the Invention

[0005] In view of the above technical problems, the present invention provides a composition containing ultra-micro vesicles with three-dimensional effects of anti-aging, whitening and repair, and a method for preparing the same. The composition has strong efficacy and low irritation, and can improve the skin from three dimensions: anti-wrinkle and firming, whitening and brightening, and soothing and repairing. Moreover, the production process is simple and easy to implement.

[0006] The technical solution adopted in this invention is as follows:

[0007] In a first aspect, the present invention provides a composition containing microvesicles, comprising the following components by weight: 0.01 to 30.0 parts of animal umbilical cord extract, 0.001 to 5 parts of a mixture of boswellic acid and azelaic acid, and 0.1 to 10 parts of nicotinamide;

[0008] In a mixture of bosine and azelaic acid, the mass ratio of bosine to azelaic acid is 2-3:2-3.

[0009] Preferably, the composition further includes 0.01 to 20 parts of a functional ingredient, wherein the functional ingredient is selected from at least one of carnosine, tetrahydromethylpyrimidine carboxylic acid, and sodium hyaluronate.

[0010] Preferably, the composition further includes 1.0 to 20.0 parts of a humectant and 0.01 to 1.0 parts of a preservative.

[0011] Preferably, the humectant is at least one of glycerin, 1,2-butanediol or hydroxyethyl urea, and the preservative is phenoxyethanol.

[0012] A second aspect of the present invention provides a method for preparing the composition, comprising the following steps:

[0013] Weigh out the animal umbilical cord extract, the mixture of pro-xylene and azelaic acid, niacinamide, functional ingredients, moisturizers and preservatives according to the parts by weight;

[0014] After dispersing the humectant in water, phase A is obtained;

[0015] The composition is obtained by uniformly mixing phase A with animal umbilical cord extract, a mixture of boswellic acid and azelaic acid, nicotinamide, functional ingredients and preservatives.

[0016] Preferably, the specific operation process of the preparation method of the composition is as follows:

[0017] After dispersing the humectant in water, stir it evenly at 80-85℃ to obtain phase A;

[0018] After cooling phase A to room temperature, it is mixed evenly with animal umbilical cord extract, a mixture of boswellic acid and azelaic acid, nicotinamide, functional ingredients and preservatives to obtain the composition.

[0019] In a third aspect, the present invention provides the use of the composition in the preparation of products for anti-wrinkle firming, whitening and brightening, and soothing and repairing.

[0020] In a fourth aspect, the present invention provides a product having three-dimensional effects of anti-wrinkle firming, whitening and brightening, and soothing and repairing, comprising the aforementioned composition.

[0021] Preferably, the product is a cosmetic.

[0022] Preferably, the cosmetic is a lotion, cream, liquid mask, or balm mask.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] This invention combines umbilical cord extract microvesicles, a mixture of pro-xylene and azelaic acid, and niacinamide to achieve a synergistic effect. When applied to the skin, this composition provides better and more comprehensive effects in three dimensions: anti-wrinkle and firming, whitening and brightening, and soothing and repairing. The composition provided by this invention has a simple production process, is highly operable, and is easy to implement. Attached Figure Description

[0025] Figure 1 This is a trend chart of IL-1α levels. Detailed Implementation

[0026] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto.

[0027] Unless otherwise specified, all reagents used in the following examples are commercially available and commonly used reagents.

[0028] Unless otherwise specified, the experimental methods used in the following embodiments are conventional operating methods in the art.

[0029] The in vitro animal umbilical cord extract used in this invention was purchased from Shaanxi Future Meat and Diet Health Technology Co., Ltd., the Bosein-Azelaic acid (i.e., a mixture of Bosein and azelaic acid) was purchased from Shaanxi Future Bio-Matrix Engineering Co., Ltd., the nicotinamide was purchased from DSM, and the phenoxyethanol was purchased from ASHLAND.

[0030] The animal umbilical cord extract used in this invention contains ultramicrovesicles (exosomes). Exosomes are small, cup-shaped vesicles with a diameter of approximately 30–100 nm, secreted by various cells, and possessing a double-membrane structure. Exosomes can transport proteins, mRNA, microRNA, and other active substances between cells, participating in many important physiological and pathological processes, and their unique roles have attracted increasing attention. Cells secrete exosomes under normal physiological conditions. Due to the fusion between the plasma membrane and multivesicular bodies, exosome-like vesicles are secreted into the extracellular space through exocytosis. Exosome-like vesicles are divided into an inner and outer layer by a lipid bilayer. Because they contain membrane lipids, membrane proteins, genetic material, and cytoplasmic components, they indirectly reflect the properties and state of the cell. At the same time, exosome-like vesicles can repair damaged cells and exert corresponding effects by binding with other cells and tissues. They also act as extracellular transport proteins mediating cell-cell communication, transferring membrane components, mRNA, miRNA, proteins (growth hormones, cytokines, etc.), and other functional components to recipient cells, enabling these components to exert their effects.

[0031] Pro-Xylane, chemically known as hydroxypropyltetrahydropyranotriol, is a xylose derivative with anti-aging activity, commonly used in cosmetics. Studies have shown that Pro-Xylane can affect the extracellular matrix in the three layers of the skin, influencing the secretion of glycosaminoglycans, promoting the synthesis of glycosaminoglycans in the skin, improving the adhesion between the dermis and epidermis, promoting the regeneration of damaged tissue, and helping to maintain the elasticity of the dermis. It can effectively keep the skin firm and smooth, and delay skin aging.

[0032] Niacinamide: It is a derivative of vitamin B3 and a recognized whitening ingredient in the field of cosmetic dermatology. It can downregulate the transport of melanin from melanocytes to melanosomes in keratinocytes, thereby inhibiting tyrosinase activity and cell proliferation. While promoting the absorption of active ingredients, it also has a whitening effect.

[0033] The raw material composition of the various embodiments and comparative examples of the present invention is shown in Tables 1 and 2.

[0034] Table 1. Raw material composition of the compositions in each embodiment (unit: weight percentage %)

[0035]

[0036] Note: In Table 1, the mass ratio of Bosein to azelaic acid in the mixture is 2:3; as another embodiment, the mass ratio of Bosein to azelaic acid can also be 1:1 or 3:2. Since the mixing ratio of the two has little effect on the effect of the composition, they are not listed here.

[0037] Table 2. Raw material composition of each comparative example composition (unit: weight percentage %)

[0038]

[0039]

[0040] The compositions in Tables 1 and 2 were prepared according to the following methods:

[0041] Weigh out the animal umbilical cord extract, mixture of boswellic acid and azelaic acid, niacinamide, functional ingredients, moisturizers and preservatives according to the corresponding formula amounts in the table;

[0042] After dispersing the humectant in water, it is stirred evenly at 85°C (at this temperature or a temperature close to this temperature, the humectant can be more evenly dispersed in water, and microorganisms in the water can also be killed) to obtain phase A;

[0043] After cooling phase A to room temperature, add animal umbilical cord extract (i.e., microvesicles, which are unstable at higher temperatures and therefore need to be mixed with phase A and other substances at room temperature), a mixture of bosine and azelaic acid, nicotinamide, functional ingredients and preservatives, and stir for 30 minutes to mix evenly.

[0044] Since the compositions prepared in Examples 1 to 7 have basically the same effects on anti-wrinkle firming, whitening and brightening, and soothing and repairing, the following description will only use the composition prepared in Example 7 as an example to illustrate the effects.

[0045] Effect test

[0046] 1. Anti-wrinkle and firming efficacy test:

[0047] 1.1 Experimental Subjects

[0048] Comparative Examples 1-6, Example 7

[0049] 1.2 Experimental Methods

[0050] For the human efficacy test, 105 volunteers aged 35-65 years were recruited, divided into groups of 15. Comparative Examples 1-6 and Example 7 were used respectively. Before the test, the subjects washed their faces and stayed in a constant temperature and humidity environment (temperature 21-23℃, humidity 55-65%) for 30 minutes. Facial photographs were taken using VISIA-CR as initial data. The subjects applied the corresponding test product to their faces twice a day, morning and evening, for 30 consecutive days. On day 0 and day 30, facial wrinkles were tested using VISIA-CR after washing the face, and skin elasticity was tested using a German CK instrument. The test results are shown in Table 3.

[0051] Table 3. Test results of the anti-wrinkle and firming effects of the compositions of the present invention.

[0052]

[0053] As can be seen from the results in Table 3, Comparative Examples 1 to 6 only contained one or two of the ingredients, and their anti-wrinkle and firming effects were not very obvious after 4 weeks of use. However, Example 7 had a better anti-wrinkle and firming effect, indicating that a significant synergistic effect was produced in anti-wrinkle and firming through the synergistic action of microcapsules ((animal) umbilical cord extract), β-Xylane-azelaic acid (a mixture of hydroxypropyl tetrahydropyranotriol and azelaic acid), and nicotinamide.

[0054] 2. Whitening and brightening efficacy test:

[0055] 2.1 Experimental Subjects

[0056] Comparative Examples 1-6, Example 7

[0057] 2.2 Experimental Methods

[0058] For the human efficacy test, 70 volunteers aged 25-45 years were recruited, divided into groups of 10. Each group used Comparative Examples 1-6 and Example 7, with each group using the same product. Before the test, participants washed their faces and remained in a constant temperature and humidity environment (temperature 21-23℃, humidity 55-65%) for 30 minutes. Skin brightness was measured using a DermaLab multifunctional skin analyzer, and the collected data served as the initial value. The average value was calculated and recorded as W0. Then, participants applied the corresponding test product to their faces twice a day, morning and evening, for four consecutive weeks. Skin brightness after four weeks was recorded as W4. The test results are shown in Table 4.

[0059] Table 4. Test results of the whitening and brightening effects of the compositions of the present invention.

[0060]

[0061] As can be seen from Table 4, Comparative Examples 1-6 did not show significant skin whitening and brightening effects, while Example 7 showed significant skin whitening and brightening effects. This indicates that the skin whitening and brightening effects are significantly enhanced by the synergistic action of microvesicles (animal umbilical cord extract), β-Xylane-azelaic acid (a mixture of hydroxypropyl tetrahydropyranotriol and azelaic acid), and niacinamide.

[0062] 3. Soothing and repairing efficacy test:

[0063] 3.1 Validation of in vitro skin models

[0064] 3.1.1 Experimental Subjects

[0065] Comparative Examples 1-6, Example 7

[0066] 3.1.2 Experimental Methods

[0067] A 3D epidermal skin model was stimulated with the anionic surfactant sodium lauryl sulfate (SLS). An in vitro skin injury model was constructed, and the soothing efficacy of the test samples was evaluated by detecting changes in the pro-inflammatory factor IL-1α in the skin model after sample treatment. The test protocol is shown in Table 5. The test results are shown in Table 6. Figure 1 As shown.

[0068] Table 5 Soothing Test Protocol

[0069]

[0070] Table 6. Test results of the soothing effect of the compositions of the present invention.

[0071]

[0072]

[0073] Note: When performing statistical analysis using the t-test method, significance compared with the BC group is indicated by #, with P-value < 0.05 indicated by # and P-value < 0.01 indicated by ##; significance compared with the NC group is indicated by *, with P-value < 0.05 indicated by * and P-value < 0.01 indicated by **.

[0074] Table 6 shows that the IL-1α content in the NC group was significantly higher than that in the BC group, indicating that the stimulation conditions in this test were effective. The IL-1α content in the PC group was significantly lower than that in the NC group, indicating that the positive control in this test was effective. Compared with the NC group, nicotinamide alone did not significantly reduce the IL-1α content. The experimental groups containing a mixture of pro-xylene and azelaic acid and microvesicles showed a certain degree of reduction in IL-1α content, but it was not significant. The combination containing microvesicles (animal umbilical cord extract), pro-xylene-azelaic acid (hydroxypropyl tetrahydropyranotriol, azelaic acid), and nicotinamide significantly reduced the IL-1α content, with an inhibition rate of 52.96%. This indicates that the synergistic effect of microvesicles (animal umbilical cord extract), pro-xylene-azelaic acid (a mixture of hydroxypropyl tetrahydropyranotriol and azelaic acid), and nicotinamide produced a significant synergistic effect in terms of soothing.

[0075] 3.2 Human Verification

[0076] 3.2.1 Experimental Subjects

[0077] Comparative Examples 1-6, Example 7

[0078] 3.2.2 Experimental Methods

[0079] Seventy volunteers with sensitive or allergic skin, aged 28-55, were selected and divided into seven groups of ten. Each group used Comparative Examples 1-6 and Example 7, respectively, twice daily (morning and evening), and comparisons were made after four weeks. Transepidermal water loss rate was tested using a German CK instrument, and the average value was calculated. W0 represents the transepidermal water loss rate before use, and W4 represents the transepidermal water loss rate after four weeks of continuous use. The test results are shown in Table 7.

[0080] Table 7. Test results of the repair effect of the composition of the present invention.

[0081]

[0082] As can be seen from Table 7, Comparative Examples 1-6 did not significantly reduce the transepidermal water loss rate in the test area, while Example 7 significantly reduced the transepidermal water loss rate in the test area. This indicates that the synergistic effect of ultramicrovesicles (animal umbilical cord extract), β-Xylane-azelaic acid (a mixture of hydroxypropyl tetrahydropyranotriol and azelaic acid), and niacinamide produces a significant synergistic effect in skin repair.

[0083] While the specific embodiments of the present invention have been described above, they are not intended to limit the scope of protection of the present invention. Those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without creative effort based on the technical solutions of the present invention are still within the scope of protection of the present invention.

Claims

1. A composition comprising ultramicron vesicles, characterized in that, It comprises the following components by weight fraction: animal umbilical cord extract 0.01-30.0 parts, mixture of bovine and azelaic acid 0.001-5 parts, nicotinamide 0.1-10 parts; The mass ratio of bovine and azelaic acid in the mixture of bovine and azelaic acid is 2-3:2-3; The composition further comprises 0.01-20 parts of functional ingredients selected from at least one of carnosine, tetrahydro methyl pyrimidine carboxylic acid, and sodium hyaluronate; The composition further comprises a humectant and a preservative, the humectant being at least one of glycerol, 1,2-butanediol or hydroxyethyl urea, and the preservative being phenoxyethanol.

2. The composition of claim 1, wherein, It further comprises 1.0-20.0 parts of a humectant and 0.01-1.0 parts of a preservative.

3. A process for the preparation of a composition according to claim 2, characterized in that, It comprises the following steps: The animal umbilical cord extract, the mixture of bovine and azelaic acid, the nicotinamide, the functional ingredients, the humectant and the preservative are weighed by weight fraction; The humectant is dispersed in water to obtain phase A; Phase A is mixed with the animal umbilical cord extract, the mixture of bovine and azelaic acid, the nicotinamide, the functional ingredients and the preservative to obtain the composition.

4. The production method according to claim 3, characterized by, The specific operation process is: The humectant is dispersed in water and stirred uniformly at 80-85°C to obtain phase A; Phase A is cooled to room temperature and mixed with the animal umbilical cord extract, the mixture of bovine and azelaic acid, the nicotinamide, the functional ingredients and the preservative to obtain the composition.

5. Use of the composition of any one of claims 1 or 2 in the preparation of a product for anti-wrinkle firming, whitening, brightening and soothing repair.

6. A product with three-dimensional effects of anti-wrinkle tightening, whitening and soothing repair, characterized in that, It comprises the composition of claim 1 or 2.

7. The product of claim 6, wherein, The product is a cosmetic.

8. The product of claim 7, wherein, The cosmetic is an emulsion, a cream, a mask liquid or a mask paste.

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