Hair care product based on plant extracts

Abstract

The invention relates to a hair composition for cutaneous and follicular penetration, characterized in that it comprises: (A) 45% to 95% by weight of exosomes derived from plant stem cells, (B) 2% to 40% by weight of a fruit extract from the Serenoa palm, (C) 1% to 15% by weight of a hair root culture extract from Ocimum Basilicum, (D) 0.05% to 10% by weight of Niacinamide, (E) 0.05% to 10% by weight of Rosemary essential oil, the weight percentages being expressed as a percentage of the sum of the weights of (A), (B), (C), (D), and (E), as well as its use in the prevention or reduction of hair loss, the cosmetic use of the composition to increase hair density or hair diameter, or a cosmetic hair treatment method comprising the application of the composition to the scalp scalp massage, possibly followed by a scalp massage.

Description

Title of the invention: Hair composition based on plant extracts. Technical field

[0001] The present invention relates to the field of hair treatment.

[0002] It relates more particularly to a hair composition based on plant extracts that increases hair density, increases hair diameter, or prevents or reduces hair loss. Previous technique

[0003] Hair grows from follicles located in the scalp.

[0004] Structure and development of the hair follicle:

[0005] The hair follicle is composed of a diverse assembly of approximately 20 distinct cell types, each with specialized functions. The hair follicle regulates hair growth through a complex interaction between hormones, neuropeptides, and immune cells. This complex interaction induces the hair follicle to produce different types of hair, as observed on different parts of the body. Hair follicles emerge through a dynamic interaction between the dermis and the epidermis, orchestrated by a sequence of cellular interactions: - Dermo-epidermal interaction: the development of the hair follicle begins with the interaction between the dermal and epidermal layers of the skin. An initial signal from the epidermis triggers this process. - Fibroblast activation: In response to the epidermal signal, fibroblasts, specialized cells in the dermis, become activated. They organize and transmit signals to neighboring keratinocytes. - Formation of the epidermal plaque: activated keratinocytes react by forming an epidermal plaque. This structure begins to push downwards, penetrating the dermal layer. - Formation of the primary bud: as the epidermal plate extends into the dermis, it forms a primary bud. This primary bud serves as the fundamental structure for the future hair follicle. - Formation of the dermal papilla: the primary bud generates signals that act as messages, influencing the surrounding fibroblasts. These messages lead to the stabilization and organization of the fibroblasts, giving rise to the dermal papilla. - Differentiation into a hair follicle: over time, the primary bud, under the continuous influence of signals from the dermal papilla, undergoes gradual differentiation. This process ultimately leads to the formation of a fully functional hair follicle.

[0006] In summary, the development of a hair follicle is a finely orchestrated process, directed by a cascade of signaling events between the epidermis and the dermis, involving fibroblasts, keratinocytes and the formation of key structures such as the epidermal plate and the dermal papilla.

[0007] As in many systems of the human body, the precise chemical pathways and roles involved in hair growth are not fully understood. However, it is known that hair growth is facilitated by the physical interaction between the dermis and epidermis within the dermal papilla, where keratinocytes and fibroblasts are concentrated. The dermal papilla is an area particularly rich in collagens and glycosaminoglycans, which maintain close contact between the two cell populations and promote the chemical communication necessary for hair follicle growth. Type IV collagen and laminin 5 are important proteoglycans because they constitute the basement membrane, the attachment zone between the epidermis and the dermis, and, in the case of hair, between the root sheath and the dermis.

[0008] A hair follicle consists of: - The papilla is a large structure located at the base of the hair follicle. The papilla is primarily composed of connective tissue and a capillary loop. Cell division within the papilla is either rare or nonexistent. - The hair matrix is ​​located around the papilla. - A root sheath composed of an outer and inner root sheath. The outer root sheath appears empty with cuboid cells when stained with an H&E dye. The inner root sheath is composed of three layers: the Henle layer, the Huxley layer, and an inner cuticle that is continuous with the outermost layer of the hair fiber. - The protrusion is located in the external root sheath at the point where the arrector pili muscle inserts. It houses several types of stem cells, which supply the entire hair follicle with new cells and participate in the healing of the epidermis after an injury.

[0009] Other structures associated with the hair follicle include the cup in which the follicle grows, called the infundibulum, the arrector pili muscles, the sebaceous glands, and the apocrine sweat glands. Receptors in the hair follicle detect the position of the hair.

[0010] Attached to the follicle is a small bundle of muscle fibers called the arrector pili muscle. This muscle is responsible for raising the hair follicle more perpendicularly to the skin surface, thus causing the follicle to protrude slightly above the surrounding skin (piloerection) and a pore surrounded by sebum. This process results in the formation of goosebumps.

[0011] Also attached to the follicle is a sebaceous gland, which produces the oily or waxy substance called sebum. The higher the hair density, the more sebaceous glands there are.

[0012] Hair structure and growth:

[0013] Outside the hair, flat, scaly cells form the cuticle, while at Inside, the cortex is composed of elongated cells which, in turn, are made up of an organized amalgam of sulfur-rich proteins arranged in an alpha helix structure. Keratin, which constitutes 50% of the hair's proteins, is the protein responsible for the shape and strength of hair. It is primarily composed of glycine, leucine, and cysteine.

[0014] The hair shaft, which is the visible part of the hair, originates near the infundibulum, at the opening of the sebaceous canal. In adults, it has a diameter ranging from 70 to 100 sqm and is composed of three well-defined layers.

[0015] The medulla, also called the pith, which occupies the central part of the hair shaft. The medulla is made up of large, anucleate, lightly pigmented cells.

[0016] The cortex plays an essential role within the hair, representing approximately 90% of its total weight. It is composed of tightly bound, spindle-shaped keratinized cells, forming a solid network. The melanocyte pigments, which determine hair color, are located within the cortex.

[0017] The cuticle is the outermost layer of the hair shaft. It is composed of keratinized cells called "scales" which are held together by a lipid binder. These scales interlock like roof tiles, with their free edges pointing towards the tip of the hair. Their main function is to protect the cortex from the various aggressions to which hair is likely to be exposed.

[0018] Hair growth occurs in cycles and comprises four phases: The Anagen phase is the longest of the cycle, lasting approximately 2 to 5 years, and is the phase during which hair actively grows. This stage accounts for 85% to 90% of all hair growth. It can be disrupted by various factors, including nutritional deficiencies, stress, and genetic predispositions. The catagen phase is a short, transitional stage that begins at the end of the anagen phase and lasts approximately 2 to 3 weeks. This phase accounts for 5% of all hairs, during which hair growth stops. Various factors, including mineral deficiencies, blood circulation, and oxygen supply, influence this phase. Insufficient factors, as well as genetic predispositions, can disrupt the Catagen phase. - The telogen phase is the resting phase and generally represents 6% to 8% of all hairs. This phase lasts approximately 100 days, during which the hair follicle is at rest and the hair in the telogen phase is fully formed. - The exogenous phase is the final phase of the hair cycle. This phase involves the growth of new hairs in the follicles and the beginning of the shedding of old hairs.

[0019] The duration of a cycle varies considerably, with an average ranging between 3 and 5 years. This period depends on several factors, such as the sex of the individual, as well as the nature and specific location of the hair follicle.

[0020] [Tables 1] Anagen phase Telogen phase Duration (months) Proportion (%) Duration (months) Proportion (%) Hair 24-72 80-90 3-6 10-15 Eyebrows 1-2 10 3 90 Pubic hair 4-8 30 12 70 Arms 3-12 20 3-12 80

[0021] The duration of the phases of the hair cycle varies according to the location of the hair follicles.

[0022] Each hair follicle goes through approximately 20 to 30 distinct cycles during its life, and its development is autonomous and independent of others.

[0023] Hair loss:

[0024] Hair loss can be attributed to a wide range of factors, such as genetics, diseases, nutritional deficiencies, aging, hormonal changes and stress, which lead to alterations in the cycles of hair follicles, including a shortening of the anagen phase, an increase in the number of hair follicles in the telogen phase and changes in the morphology of hair follicles.

[0025] The most common type of hair loss is androgenic alopecia, also known as male pattern baldness. This condition affects millions of people, both men and women. Recently, the incidence of hair loss has increased considerably among young people. Male pattern baldness is usually caused by the conversion of testosterone to dihydrotestosterone, which reduces the size of hair follicles and the blood vessels that support them, ultimately by causing the closure or even death of certain follicles. The conversion of testosterone to dihydrotestosterone is triggered by the hormone 5α-reductase. It is also known that vitamin H (biotin) deficiencies can lead to thinning hair.

[0026] It has been suggested that effective strategies to combat hair loss include prolonging the anagen phase, stimulating the telogen-anagen transition of follicles, reversing miniaturization and improving the viability of dermal papilla cells to stimulate the generation of new follicles.

[0027] Minoxidil (MNX) and finasteride are currently treatments for hair loss in patients with alopecia in many countries. However, their therapeutic benefits are limited due to their temporary and undesirable effects.

[0028] Nevertheless, consumers are increasingly seeking effective natural alternatives, particularly those based on plant extracts, capable of treating hair loss or increasing hair density or diameter. Summary

[0029] The invention relates to a hair composition for cutaneous and follicular penetration characterized in that it comprises: (A) 45% to 95% by weight of exosomes derived from plant stem cells, (B) 2% to 40% by weight of a fruit extract from the palm tree of the genus Serenoa, (C) 1% to 15% by weight of a cultured extract of hairy roots of Ocimum Basilicum, (D) 0.05% to 10% by weight of Niacinamide, (E) from 0.05% to 10% by weight of Rosemary essential oil, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0030] Another aspect relates to the composition according to the invention for its use in the prevention or reduction of hair loss.

[0031] Another aspect relates to the cosmetic use of the composition according to the invention to increase hair density, or increase the diameter of hair.

[0032] Another aspect relates to a cosmetic hair treatment process comprising the application of the composition according to the invention to the scalp, possibly followed by a scalp massage or friction.

[0033] Without wishing to be bound to any particular theory, exosomes derived from plant stem cells appear to act as vesicular vectors, transporting the active ingredients of the composition to the papilla of the hair follicles. The small size and liposomal envelope of these exosomes give them a capacity to Optimal penetration through the scalp and pilosebaceous orifices. Thanks to the protection provided by their lipid membrane, they also help preserve these active ingredients during application to the scalp by friction.

[0034] The composition according to the invention comprises a mixture of active ingredients advantageously chosen for their beneficial effects on the development of the hair follicle: - Reduction of dihydrotestosterone (DHT), a hormone associated with hair loss, by inhibiting the activity of 5α-reductase. This inhibition helps to preserve the health and longevity of hair follicles. - Improved blood circulation to the dermis, the deeper layer of skin that houses the hair follicles. Better circulation helps deliver essential nutrients and oxygen to the hair follicles, promoting their growth and vitality. - Optimal hair rooting in the skin, which helps prevent premature hair loss and promotes the maintenance of healthy hair. - Healthy scalp: the composition according to the invention promotes scalp hydration, helps maintain the perfect moisture balance, creating an ideal environment for healthy hair growth.

[0035] Exosomes derived from plant stem cells and active ingredients act synergistically to give the composition excellent skin and follicular penetration, excellent tolerance and efficacy that goes beyond current treatments.

[0036] The composition according to the invention makes it possible to promote hair growth, increase hair density and / or thickness, increase hair resistance, reduce scalp inflammation and protect it from environmental stressors, thus promoting a healthier environment more conducive to hair growth.

[0037] The composition according to the invention also has the advantage of being very stable during storage and not degrading during handling. This is a very important advantage given that the integrity of the membrane of plant-derived exosome-type nanoparticles is crucial for their bioactivity.

[0038] The features described in the following paragraphs may optionally be implemented independently of each other or in combination with each other: Brief description of the figures

[0039] Other features, details and advantages will become apparent upon reading the detailed description below, and upon analysis of the accompanying drawings, on which:

[0040] [Fig.1]

[0041] [Fig.1] shows the evaluation of hair density by measuring the number of hairs per cm2 on 1cm2 of shaved scalp (values ​​expressed as median ± SE; p<0.05 by t-test).

[0042] [Fig.2]

[0043] [Fig.2] shows the evaluation of the regrowth rate in 48 hours on 1cm2 of shaved scalp (values ​​expressed as median ± SE; / ?<0.05 by t-test).

[0044] [Fig.3]

[0045] [Fig.3] shows the evaluation of the anagen and telogen hair ratio in % over 1cm2 of shaved scalp (values ​​expressed as median ± SE; p<0.05 by t-test).

[0046] [Fig.4]

[0047] [Fig.4] shows the evaluation of terminal and vellus hair density per cm2 on 1cm2 of shaved scalp (values ​​expressed as median ± SE; p<0.05 by t-test). Detailed description

[0048] The hair composition according to the invention is characterized in that it comprises the following ingredients of plant origin: (A) 45% to 95% by weight of exosomes derived from plant stem cells, (B) 2% to 40% by weight of a fruit extract from the palm tree of the genus Serenoa, (C) 1% to 15% by weight of a cultured extract of hairy roots of Ocimum Basilicum, (D) 0.05% to 10% by weight of Niacinamide, (E) from 0.05% to 10% by weight of Rosemary essential oil, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0049] (A) Exosomes derived from plant stem cells:

[0050] Plant exosomes are nanoparticles produced by the multivesicular corpuscles of most plant or fruit stem cells, measuring between 30 and 200 nm. They are extruded from the stem cells from which they originate into the extracellular matrix, where they act as messengers, facilitating communication, intercellular signaling, and the transport of biomaterials involved in regulating the expression of cellular functions. Their native cargo consists of various proteins and RNAs forming the cytosol, surrounded by a protective lipid membrane. Numerous extraction methods have been developed in recent years, each with its advantages and disadvantages; the most effective are those that allow for high vesicle purity while maintaining their integrity.

[0051] The principle of exosome extraction consists of sequential steps where ultracentrifugation and ultrafiltration play a major role because they are adapted to the nano-size, shape and density of these extracellular vesicles.

[0052] Ultracentrifugation is a standard of excellence in the production of plant exosomes. It is a differential centrifugation, based on the separation of particles according to their density, size and shape by using a series of high-speed centrifugations in a homogeneous medium such as a phosphated physiological solution, preceded by an initial preparation of the sample to remove larger cells and debris.

[0053] Further purification is often necessary to remove unwanted debris such as certain proteins, RNA and membrane aggregates, which can be achieved by gradient ultracentrifugation.

[0054] The following ultrafiltration is a method isolating exosomes according to their size, molecular weight and hydrodynamic diameter by means of a porous rectangular channel where the smaller particles are once again dissociated from the larger ones.

[0055] According to one embodiment, exosomes from plant stem cells are extracted in the following manner: (a) Preparation of plant stem cell lines in the laboratory, (b) Stem cell culture and amplification, (c) Observation of their differentiation by electron microscopy, (d) Series of progressive ultracentrifugations, (e) Separation of the particles obtained by successive ultrafiltrations in order to obtain exosomes of high purity and, (f) High-pressure homogenization of the material obtained, extraction of vesicles and liposomal stabilization of their contents.

[0056] Plant-derived nanoparticles obtained through these processes have the potential to be stable and resistant to the scalp microenvironment thanks to the lipids contained in their membrane, and to transport active ingredients through the stratum corneum. The processes used also give the formulations significant stability that does not degrade during the handling and storage of the final cosmetic product preparations.

[0057] Exosomes derived from plant stem cells can be selected from exosomes derived from stem cells of flowering plants, in particular from the following families: - Curcubitaceae, in particular of the Lagenaria genus, such as Lagenaria siceraria, of the Sicyos genus, such as Sicyos angulatus, or of the Benincasa genus, such as Benincasa hispida; - Rosaceae, especially of the genus Malus, such as Malus domestica, of the genus Prunus, such as Prunus armeniaca or Prunus dulcis, or of the genus Rosa, such as Rosa gallica or Rosa canina.

[0058] It is possible to use mixtures of exosomes.

[0059] The content of exosomes from plant stem cells in the hair composition according to the invention is 45% and 95% by weight, in particular 60% to 94% by weight, in particular 75% to 93% by weight, more particularly 85% to 92% by weight, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0060] (B) Extract of the fruit of the palm tree of the genus Ser enoa:

[0061] The genus Serenoa comprises a single species in the Arecaceae family, Serenoa repens (heterotypic synonym: Serenoa serrulala), which is native to the southeastern United States, particularly Florida and its subtropical climate. It is a dwarf palm species that can reach 3 meters in height and is more commonly known as "saw palm" or "Florida fan palm." Its fruits are globose-ellipsoid drupes, green, then orange, and finally black when ripe.

[0062] The fruit extract of this palm tree is used in the composition according to the invention for its ability to block 5α-reductase, resulting in a decrease in DHT levels. This botanical extract plays an essential role in addressing the problem of hair loss.

[0063] The content of the fruit extract of the palm of the genus Serenoa in the hair composition according to the invention is from 2% to 40% by weight, in particular from 53% to 930% by weight, in particular from 64% to 208% by weight, more particularly from 6.5% to 7.510% by weight, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0064] (C) Ocimum Basilicum hairy root culture extract:

[0065] Basil is the common name for the culinary herb Ocimum basilicum.

[0066] The extract of hairy roots of Ocimum Basilicum can be obtained By infecting a small portion of a basil plant with the naturally occurring soil bacterium Agrobacterium rhizogenes, followed by culturing on agar plates, a natural transformation of the plant cells occurs. These cells then form tiny roots, known as hair roots. These hair roots can be propagated on artificial culture media in Petri dishes or suspended in liquid media. After sufficient biomass has been produced, the roots are harvested, and the active compounds are extracted and dissolved in a solvent to make them available for use in hair treatment formulations.

[0067] The hair root culture extract of Ocimum Basilicum offers several key advantages in combating hair loss, including: - Inhibition of 5α-reductase II activity: One of the central mechanisms by which Ocimum Basilicum contributes to hair health is by effectively inhibiting the activity of 5α-reductase II. This inhibition is crucial for reducing the conversion of testosterone to dihydrotestosterone (DHT), a hormone strongly associated with hair loss. By lowering DHT levels, Ocimum Basilicum helps maintain the health of hair follicles and can potentially slow down or prevent hair loss. - Stimulation of dermal papilla cell proliferation: Another significant benefit of Ocimum Basilicum is its ability to enhance the proliferation of dermal papilla cells within hair follicles. Dermal papilla cells play a crucial role in regulating hair growth, and increasing their proliferation can lead to stronger, healthier hair follicles, promoting hair growth and thickness.

[0068] The content of the hair root culture extract of Ocimum Basilicum in the hair composition according to the invention is from 1% to 15% by weight, from 1% to 10% by weight, in particular from 1.5% to 7% by weight, more particularly from 1.5% to 4% by weight, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0069] (D) Niacinamide:

[0070] Niacinamide (also called nicotinamide) is one of the three forms that vitamin B3 can take, the other two being niacin and nicotinamide riboside. It produces the following multiple effects: - Keratin synthesis: Niacinamide plays a crucial role in the synthesis of keratin, a protein that forms the structural basis of hair. By promoting keratin production, it contributes to the overall health and strength of hair strands. - Anti-inflammatory properties: The anti-inflammatory properties of niacinamide are beneficial for soothing and calming the scalp. This can relieve discomfort and irritation often associated with various scalp conditions, thus promoting a healthier environment for hair growth. - Improved blood circulation: Niacinamide improves blood circulation, which is essential for delivering vital nutrients and oxygen to hair follicles. Better circulation can stimulate the follicles and promote healthier hair growth. - Redness reduction: By reducing redness and irritation of the scalp, niacinamide helps create a more comfortable and supportive environment for hair follicles, which can potentially reduce hair loss. - Promoting hair growth: Niacinamide is linked to promoting hair growth by nourishing hair follicles and stimulating their activity. This can lead not only to increased hair growth, but also to thicker, healthier strands. - Reduction of hair loss: Niacinamide's ability to support hair follicles and reduce inflammation may contribute to a decrease in hair loss, thus helping to maintain a thicker head of hair. - Improved hair thickness: With continued use, niacinamide can contribute to thicker, stronger hair. - Moisture retention: Niacinamide helps retain moisture, thus preventing dryness and brittleness. Hydrated hair is less prone to damage and breakage. - Environmental protection: Niacinamide acts as a protective shield against environmental damage, such as pollution and UV rays. This helps maintain hair integrity and prevents it from becoming brittle and fragile.

[0071] The niacinamide content in the hair composition according to the invention is 0.05% to 10% by weight, in particular 0.1% to 8% by weight, in particular 0.2% to 5% by weight, more particularly 0.3% to 1% by weight, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0072] (E) Rosemary essential oil:

[0073] Rosemary, Salvia rosmarinus, is a species of shrub in the family Lamiaceae (or Labiatae), growing wild around the Mediterranean, particularly in arid and rocky garrigue, on calcareous soils4.

[0074] Rosemary essential oil has multiple properties. It is incorporated into the composition according to the invention primarily to produce the following two effects: - Vasodilatory properties: Rosemary essential oil possesses impressive vasodilatory properties, meaning it helps dilate blood vessels. This action can improve blood circulation in the scalp, ensuring that hair follicles receive an adequate supply of nutrients and oxygen. Improved circulation can stimulate hair follicles, which can promote healthier hair growth. - Reduction of 5α-reductase: Another significant benefit of rosemary essential oil is its ability to reduce the activity of 5α-reductase. By inhibiting this This enzyme helps reduce levels of dihydrotestosterone (DHT), a hormone strongly linked to hair loss. This reduction in DHT levels can promote the maintenance of healthy hair follicles and potentially slow down or prevent hair loss.

[0075] By synergistically exploiting these properties, the inclusion of rosemary essential oil in the hair composition according to the invention offers a holistic approach to treating hair loss. It not only promotes better blood circulation to nourish the hair follicles, but it also addresses the root cause of hair loss by reducing DHT levels, thus potentially offering individuals a comprehensive solution for maintaining and regenerating their hair.

[0076] The content of rosemary essential oil in the hair composition according to the invention is 0.05% to 10% by weight, in particular 0.08% to 8% by weight, in particular 0.1% to 5% by weight, more particularly 0.15% to 1% by weight, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

[0077] Other ingredients:

[0078] The hair composition according to the invention may include a physiologically acceptable liquid medium, such as an aqueous, alcoholic or hydroalcoholic solvent.

[0079] The alcoholic solvent is chosen for example from lower alcohols having from 1 to 8 carbon atoms and in particular 1 to 6 carbon atoms, such as ethanol, isopropanol, propanol, butanol; polyethylene glycols having from 6 to 80 ethylene oxides; polyols such as propylene glycol, isoprene glycol, butylene glycol, glycerin, sorbitol, 1,3-propanediol.

[0080] When the composition includes an aqueous solvent, it has a pH compatible with the skin, preferably ranging from 3 to 8 and better from 4 to 7.

[0081] When the composition includes an aqueous or hydroalcoholic medium, it is possible to add a fatty (or oily) phase to this medium.

[0082] The hair composition according to the invention is in particular a composition intended for topical application to the scalp and / or hair.

[0083] The composition according to the invention may also include one or more additional compounds selected in particular from a vitamin, a humectant or moisturizing agent, a conditioner, a vasodilator (e.g., Mentha piperita essential oil), an antiseptic agent, an antimicrobial agent, an antioxidant agent (e.g. extract of Faex) and / or a pH regulator, an emulsifying agent, a surfactant, in particular selected from non-ionic, anionic, cationic and amphoteric surfactants, a gelling agent, a viscosity control agent, polymeric or not, natural or synthetic, a bulking agent, a coloring agent and / or perfumer, a UV filter, a filler such as mother-of-pearl, titanium dioxide, resins and clays, a peptizer, an acidic agent, an alkaline agent, a reducing agent, an oxidizing agent, an additional active ingredient different from the aforementioned active ingredients intended to further improve the activity on hair loss and / or on slowing down hair loss, and which has already been described for this activity, such as nicotinic acid esters, including in particular tocopherol nicotinate, benzyl nicotinate and C1-C6 alkyl nicotinates such as methyl or hexyl nicotinates; hair regrowth promoting agents selected from pyrimidine 3-oxide derivatives such as 2,4-diaminopyrimidine-3-N-oxide (Aminexil), pyridine-dicarboxylate derivatives or one of its salts such as pyridine-2,4-dicarboxylate dimethyl ester; and a mixture of these compounds.

[0084] The vitamin can be chosen from the B group such as: B1, B2, B5 (panthenol), B6, B7, B8, B12, a vitamin C, a vitamin E, or a mixture thereof.

[0085] The humectant or moisturizing agent may be chosen from: glycerin, aloe vera, soybean germ extract, glycosaminoglycan, retinyl palmitate, ferric sulfate or a mixture thereof.

[0086] The conditioner can be chosen from: hydrolyzed keratin, coconut extract, sunflower seed oil.

[0087] The above additional compounds may each be present in quantities ranging from 0.000001% to 20% by weight relative to the total weight of the composition.

[0088] The composition according to the invention can be presented in all the galenic forms normally used in the cosmetic field, such as a lotion, a serum, a shampoo, a conditioner, a dry shampoo, a gel, a wax, a lacquer, a spray, a mousse, a paste, a cream, a powder, an oil, a mask, a treatment, or a toner.

[0089] It can be formulated as an aqueous or hydro-alcoholic solution or suspension, an emulsion or dispersion of liquid or semi-liquid consistency obtained by dispersing a fatty phase in an aqueous phase (O / W) or vice versa (W / O), a dispersion or emulsion of soft consistency, an aqueous or hydro-alcoholic gel, or even microcapsules or microparticles, or vesicular dispersions of ionic and / or non-ionic type.

[0090] According to a preferred embodiment, the composition is in the form of a lotion or serum.

[0091] Applications:

[0092] The composition according to the invention is preferably applied to the scalp. After this application, the scalp may be massaged or rubbed to promote the penetration of the active ingredients of the composition through of the stratum corneum. The application time can vary from 1 to 30 minutes. The product can then be rinsed off depending on its form. For example, in the case of dry shampoo, the product does not need to be rinsed off.

[0093] The present invention also relates to the composition as described above for its use in the prevention or reduction of hair loss, the application being optionally followed by a scalp massage, and then possibly rinsing.

[0094] The present invention also relates to the cosmetic use of the composition as described above to increase hair density, or increase the diameter of hair.

[0095] The present invention also relates to a cosmetic hair treatment method comprising the application of the hair composition as described above to the scalp, the application being optionally followed by a scalp massage, and then possibly rinsing. Examples

[0096] 1. Serum according to the invention

[0097] The INCI ingredients of the serum according to the invention and their percentages by weight are presented in Table 1 below.

[0098] The preparation of the serum according to the invention is carried out at a constant temperature of 20 °C and comprises 5 main phases (cp) described in Table 2 below.

[0099] [Tables 1] Ingredients % by weight Water 87.684558 Malus Domestica Fruit Cell Culture Extract 0.200000 Serenoa Serrulata Fruit Extract 0.015000 Ocimum Basilicum Hairy Root Culture Extract 0.004500 Niacinamide 0.000750 Rosmarinus Officinalis Extract 0.000400 Glycerin 9.610000 Aloe Barbadensis Leaf Juice Powder 0.870000 Phenoxyethanol 0.536000 Isopropyl Alcohol 0.300000 Ethanol 0.150000 Faex Extract 0.150000 Glycine Soja Germ Extract 0.090000 Hydrolyzed Keratin 0.060000 PEG-40 Hydrogenated Castor Oil 0.060000 Lecithin 0.055000 Rosmarinus Officinalis Leaf Oil 0.055000 Cocos Nucifera Fruit Extract 0.050000 Mentha Piperita Oil 0.035000 Xanthan Gum 0.020000 Tocopheryl Acetate 0.011050 Sodium Benzoate 0.009000 Sodium Citrate 0.008850 Ascorbic Acid 0.005050 Citric Acid 0.005050 Ascorbyl Palmitate 0.003000 Glycosaminoglycans 0.003000 Potassium Sorbate 0.003000 Retinyl Palmitate 0.003000 Helianthus Annuus Seed Oil 0.000600 Panthenol 0.000600 Biotin 0.000300 Inositol 0.000300 Pyridoxine HCl 0.000300 Thiamine HCl 0.000300 Calcium Sulfate 0.000180 Riboflavin 0.000045 Magnesium Sulfate 0.000039 Manganese Sulfate 0.000039 Copper Sulfate 0.000030 Cyanocobalamin 0.000030 Potassium Sulfate 0.000015

[0100] [Tables2] 0 Operating procedure Ingredients A Homogenize A Aloe Barbadensis leaf juice powder Water B Make a homogenous premix and add B Glycerin Faex extract, glycine soja germ extract, hydrolyzed keratin, tocopheryl acetate, glycosaminoglycans, retinyl palmitate, ascorbyl palmitate, niacinamide, panthenol, riboflavin, biotin, inositol, thiamine HCl, pyridoxine HCl, cyanocobalamin, calcium sulfate, magnesium sulfate, copper sulfate, potassium sulfate, zinc sulfate, manganese sulfate, ferric sulfate. Add C Serenoa Serrulata Fruit Extract D Make a homogeneous premix and add D Rosmarinus Officinalis Leaf Oil Mentha Piperita Oil Glycerin, water, lecithin, cocos nucifera fruit extract, sodium citrate, ascorbic acid, citric acid, tocopherol acetate, helianthus annuus seed oil, rosmarinus officinalis extract.Add E Extract of hairy root culture of Ocimum Basilicum Extract of fruit cell culture of Malus Domestica Phenoxyethanol.

[0101] 2. Randomized, double-blind, placebo-controlled clinical evaluation of efficacy serum according to example 1).

[0102] 2.1. Objective: The objective of this clinical study is to evaluate the efficacy and safety The serum, as described in example 1, was shown to reduce hair loss, stimulate hair regrowth, and improve hair health after 12 weeks of use. The impact of this intervention was assessed quantitatively and at the individual participant level.

[0103] 2.2. Clinical study methodology: 20 men and 20 women are selected, A total of 40 participants were randomly divided into two groups identical in terms of sex and average age. Selection was based on the following predefined criteria, assessed by an expert for the degree of hair loss using scales recognized by the scientific community.

[0104] 2.2.1. Inclusion criteria: participants of both sexes aged 25 to 55 years presenting signs of mild to moderate hair loss assessed as II-III on the Ludwig scale for women (Dermatology (4th ed.). Elsevier. 2018. pp. 2637-2648) and III-IV on the Norwood-Hamilton scale for men (Guarrera M, Cardo P, Arrigo P, Rebora A, “Reliability of hamilton-norwood classification”, Int J Trichology, vol. 1, no 2, 2009, p. 120-2).

[0105] 2.2.2. Exclusion criteria: individuals undergoing medical or other treatment for the Hair loss, individuals undergoing hormone therapy.

[0106] 2.2.3. Distribution by age group:

[0107] Group A: subjects receiving the serum (25-35 years: 5 men, 6 women; 35-55 years: 5 men, 4 women).

[0108] Group B: subjects receiving placebo (25-35 years: 5 men, 6 women; 35-55 years: 5 men, 4 women).

[0109] 2.2.4. Distribution by degree of baldness

[0110] Initial assessment of participants according to the severity of hair loss and taking into account the age group [YES] [Tables 3] Scales Group A Group B Ludwig II 4 4 Ludwig III 6 6 Norwood-Hamilton III 3 3 Norwood-Hamilton IV 7 7

[0112] 2.2.5. Description of evaluation criteria and tools

[0113] Baseline and end-of-study measures are defined by the following criteria: - Counting hairs lost at 30, 60 and 90 days; - Evaluation of hair density; - Analysis of hair diameter; - Evaluation by a specialist of the degree of hair loss on international classification scales; - Self-assessment of subjects by targeted response satisfaction questionnaire (“closed-ended questionnaire”).

[0114] These measurements are taken using the following devices:

[0115] Hair loss was assessed using the SELS® (Surface Evaluation of the Living Skin) counting method developed by Professor Tronnier at the Institute of Experimental Dermatology of the University of Witten-Herdecke (Germany).

[0116] Hair photographs were analyzed using a Visioscan® VC 20plus device, which accurately measures hair density, hair diameter, and degree of hair loss. pH, hydration, and transepidermal water loss analyses were performed using the C+K Multi Probe device and its various probes, detailed below.

[0117] 2. 2. 5.a. Evaluation by Visioscan ® VC 20plus

[0118] The Visioscan® VC 20plus is a device using the SELS® assessment method which has allowed us to capture high-resolution images to characterize skin structures, to accurately measure the number, length and diameter of hairs in a shaved area and the level of hydration / dryness / scaling of the scalp as well as the sebum production of each sebaceous gland in the area studied.

[0119] Technical specifications: Image size: 10 x 8 mm; Resolution: 1 / 2 B / W CMOS sensor 1.3 MPix (1280 x 024 pixels); Light source: UV-A LED 390 nm; Lens: 20 mm; f / 2.8

[0120] 2. 2. 5.b. Evaluation by C+K Multi Probe Adapter System

[0121] This platform allows for more specific analyses by using the Skin-pH-Meter PH 905, Corneometer® Cm 825 and Tewameter® TM Nano probes for reproducible results.

[0122] 2. 2. 5.c. Evaluation by the Skin-pH- Meter PH 905

[0123] The Skin-pH-Meter PH 905 allowed us to measure the pH on the surface of the scalp and to establish that it was healthier after using our serum due to a modification of the skin microbiome. The measurement principle is based on the application to the test area of ​​two combined electrodes, one sensitive to H+ ions, the other serving as a reference, connected to a handpiece containing the electronic measuring instruments.

[0124] 2. 2. 5.d. Evaluation by Corneometer® Cm 825

[0125] The Corneometer® Cm 825 indicates the hydration level of the superficial layers of the stratum corneum on the scalp by measuring its electrical properties. The measurement principle is based on capacitance measurements of the dielectric medium. Any change in skin surface hydration leads to a modification of these dielectric constants, and consequently, of the capacitance measurement.

[0126] The probe is applied vertically to the scalp. During the measurement, an electric field penetrates the superficial layers of the skin and the dielectric constant is measured. The measurement is expressed in units from 0 to 125 according to the following indices: - 6-10: very dry scalp; - 10-50: dry scalp; - 50-125: hydrated scalp.

[0127] 2. 2. 5.e. Evaluation by Tewameter® TM Nano

[0128] The Tewameter® TM Nano is a high-precision measuring device because it can analyze transepidermal water loss expressed in g / h / m² in areas as small as 2 mm in diameter. Water evaporation through the skin is an essential element for proper metabolic function.

[0129] 2.3. Data analysis

[0130] The data and images obtained are automatically captured by the professional software TrichoSciencePro. This program integrates the diagnostic studies necessary to initiate clinical evaluation and analytical tools such as trichoscopy, phototrichogram, and dermatoscopy, enabling analysis of targeted follicular units and automated data calculation (number of hairs at each evaluation, correlated with previous measurements). It also allows for the analysis of hair density and diameter in different areas of the scalp, as well as access to their actual distribution within the follicular units. Trichoscopic evaluation is performed in predefined circular fields of view, as required for clinical trials or scientific research, since this function allows for the synchronization of symmetrical sites for evaluation, regardless of the angle used to obtain the images of the studied sites.

[0131] Measurements of hair density, hair diameter and distribution in follicular units are likewise integrated automatically, allowing for safe marking and counting.

[0132] The Phototrichogram section of this tool is widely recognized and prevalent in clinical trichology practice due to its high accuracy. We used this methodology to distinguish subclinical forms of female pattern baldness in the early stages of the disease, to perform a differential diagnosis between androgenetic alopecia (AGA) and chronic telogen effluvium (CTE), and to evaluate the efficacy of our serum. This program calculates the total number of hairs per square centimeter of skin, the quantities and percentage of telogen and vellus hairs in the telogen phase. The Phototrichogram analysis also allows for the determination of the average hair growth rate.

[0133] Values ​​are expressed as median ± SE; p<0.05 by t-test.

[0134] 2.4. Evaluation Protocol

[0135] Day 1: Each participant in Group A, representing the serum intervention arm (serum from Example 1), and each participant in Group B, representing the control arm (Placebo), are given dropper bottles of the same formulation, without any possible external identification or difference. of opacity or texture, in sufficient quantity for 4 weeks of treatment, and a single application method. All participants also receive the same neutral shampoo to use throughout the clinical study, to ensure that subjects' personal hygiene products do not interfere with the study results. These two precautions allowed us to standardize the intervention.

[0136] Days 1 to 30: Subjects apply 30 drops of the solution directly to their clean scalp every morning, massaging gently until completely absorbed. The solution is not rinsed out until the following morning. The application method is demonstrated on day 1 by a specialist, blinded to the subject's group assignment, applying the solution allocated to each subject.

[0137] Visit 1: On day 30, subjects are assessed by a specialist, unaware of their group assignments, to verify proper application of the solution and to evaluate the four-week results regarding hair loss, hair density, and hair diameter. Subjects receive a further supply of dropper bottles of the solution in sufficient quantity for the following four weeks.

[0138] From day 31 to day 61, subjects continue applying their solution according to the same protocol.

[0139] Visit 2: On day 61, subjects are assessed by a specialist unaware of their group assignments to verify proper application of the solution and to evaluate the eight-week results regarding hair loss, hair density, and hair diameter. Subjects receive a further supply of dropper bottles of the solution in sufficient quantity for the following four weeks.

[0140] From day 62 to day 92, subjects continue applying their solution according to the same protocol.

[0141] Visit 3: On day 93, subjects are assessed by a specialist who is not aware of the subjects' group assignments, in order to establish the twelve-week results on hair loss, hair density, and hair diameter.

[0142] 2.5. Observed results

[0143] The results are shown in Figures 1, 2, 3 and 4.

[0144] In subjects in the intervention arm (group A) receiving 30 drops of the anti-hair loss serum daily (serum from example 1), a significant reduction in hair loss was observed compared to the control arm (group B), quantified as the number of hairs lost during the reference period of 90 days. clinical study. The reduction in hair loss was progressive, measurements were taken at 1, 2 and 3 months of serum application with respective percentage reductions of -16%, -35% and -40%.

[0145] The stimulation of regrowth was demonstrated in the intervention arm by a clear increase in hair density in subjects who used the serum from example 1. This increase in hair density was observed at 1, 2 and 3 months of application of the serum from example 1, with respective percentages of +8%, +17% and +23%.

[0146] The mean hair diameter also increased progressively in the intervention arm, measured in mm at 1, 2 and 3 months of application of the serum from example 1 to reach +6% at the end of the study compared to the initial measurements.

[0147] Participant satisfaction in the intervention arm was also clearly positive. Subjects using HFC serum reported via closed-ended questionnaires a marked improvement in hair volume, less scalp irritation, better hair health, and a significant improvement in their perception of hair loss and their image, which they reported had a positive impact on their quality of life and socio-professional relationships.

[0148] Increased hair density is considered one of the best parameters in any clinical study evaluating hair regrowth. As such, the Comparative Scoring Criteria, widely accepted as an evaluation tool, relies on the actual increase in hair density to categorize the effects of different interventions. In our study, we used this tool for our quantitative measurements and to highlight the differences between the two groups. Thus, the intervention arm, or Group A, receiving the serum from Example 1, showed an increase in hair density, measured in hairs / cm², at the intermediate measurements at 30 and 60 days and at the final measurement at 90 days, compared to baseline measurements.

[0149] Application of the serum in Example 1 showed regrowth of >40 hairs / cm² within 3 months, which is considered excellent regrowth. In comparison, good regrowth is considered to be 20-39.99 hairs / cm², moderate regrowth 10-19.99 hairs / cm², poor regrowth 5-9.99 hairs / cm², and no regrowth or product ineffectiveness if <5 hairs / cm². The improvement in the ranking of participants in Group A on international scales also testifies to this efficacy.

[0150] 2.5.1. Long-term assessment of the degree of hair loss, according to classifications international and age groups

[0151] [Tables4] Scales Group A Group B Ludwig I 3 0 Ludwig II 5 4 Ludwig III 2 6 Norwood-Hamilton II 4 0 Norwood-Hamilton III 3 3 Norwood-Hamilton IV 3 7

[0152] 2.5. 2. Safety assessment

[0153] All topical treatments can result in side effects, whether expected or not, and of varying severity. We gave participants the opportunity to report any direct or related side effects in the questionnaires they received, in order to index them and, if necessary, compare them with other hair regrowth stimulating products available on the market.

[0154] Based on the data collected, we found no notable side effects among the treated participants. The safety of application of the serum in Example 1 is rated 'A' according to the following criteria:

[0155] [Tables5 A No side effects or very mild, transient side effects B Moderate, transient side effects C Allergic reaction, requiring discontinuation of treatment D Severe reaction requiring therapeutic action

[0156] 2.5. 3. Self-assessment results using questionnaires

[0157] [Tableauxô] Group A (n=20) Initial Assessment Day 0 Visit 1 Day 30 Visit 2 Day 60 Visit 3 Day 90 Hair Loss Score 1 (100%) 1 (70%) 2 (30%) 2 (60%) 3 (40%) 3 (80%) 4 (20%) Texture Score 1 (60%) 2 (40%) 1 (40%) 2 (30%) 3 (30%) 2 (30%) 3 (50%) 4 (20%) 3 (40%) 4 (20%) 5 (40%) Volume Score 1 (70%) 2 (30%) 1 (50%) 2 (50%) 2 (60%) 3 (40%) 4 (80%) 5 (20%) 1 (50%) Itching score: 2 (30%) 3 (20%) 1 (60%) 2 (40%) 1 (70%) 2 (30%) 1 (100%) Perceived severity of baldness score: 4 (10%) 5 (90%) 3 (70%) 4 (30%) 2 (40%) 3 (60%) 1 (80%) 2 (20%) Quality of Life score: 3 (70%) 4 (30%) 3 (80%) 4 (20%) 2 (40%) 3 (60%) 1 (60%) 2 (40%)

[0158] [Tables?] Group B (n=20) Initial Assessment Day 0 Visit 1 Day 30 Visit 2 Day 60 Visit 3 Day 90 Hair Loss Score 1 (100%) 1 (90%) 2 (10%) 1 (90%) 2 (10%) 1 (90%) 2 (10%) Texture Score 1 (90%) 2 (10%) 2 (30%) 3 (70%) 2 (20%) 3 (80%) 2 (20%) 3 (80%) Volume Score 1 (80%) 2 (20%) 1 (80%) 2 (20%) 2 (100%) 2 (100%) Itching Score 1 (50%) 2 (30%) 3 (20%) 1 (50%) 2 (40%) 3 (10%) 1 (60%) 2 (40%) 1 (60%) 2 (40%) Perceived Severity Score Baldness 4 (10%) 5 (90%) 4 (30%) 5 (70%) 4 (30%) 5 (70%) 4 (30%) 5 (70%) Quality of Life Score 4 (20%) 5 (80%) 4 (20%) 5 (80%) 4 (30%) 5 (70%) 4 (30%) 5 (70%)

[0159] Legend:

[0160] *Observed hair loss rate: 1 = more than 100, 2 = 50-100, 3 = 30-50, 4 = 10-30, 5 = less than 10.

[0161] *Hair texture assessment: 1 = rough, brittle and dry hair, 2 = dry, 3 = normal, 4 = soft, 5 = very soft.

[0162] * Volume score evaluation: 1 = very little volume, 2 = little volume, 3 = volume average, 4= good volume, 5= excellent volume.

[0163] *Itching assessment: 1= no itching, 2= mild itching, 3= moderate itching, 4= severe itching, 5= intense itching impacting daily life.

[0164] * Assessment of the degree of perceived baldness: 1= no baldness, 2= slight baldness, 3= 4 = moderate baldness, 5 = significant baldness, 5 = very visible baldness.

[0165] *Assessment of the negative impact of hair loss on Quality of Life: 1= no impact, 2= slight impact, 3= significant impact, 4= disabling, 5= very disabling

[0166] At the end of our clinical study, the self-assessment results of the participants in the intervention arm, group A, showed that: - 96% of treated participants would be willing to buy the serum from example 1 again. - 95% of treated participants found a marked improvement in the overall condition of their hair. - 92% of treated participants felt that the serum from example 1 helped to slow their hair loss. - 90% of treated participants found their hair to be stronger and less brittle. - 88% of treated participants felt their hair was thicker. - 85% of treated participants would recommend the serum from example 1 to a close friend.

[0167] 2.6. Conclusion

[0168] This clinical evaluation demonstrated the efficacy of the serum in example 1 composed of a synergistic association of active ingredients transported in the environment close to the hair follicles thanks to an optimization of their penetration for this galenic form.

[0169] Data from this clinical study indicate that the Serum in Example 1 is effective in reducing hair loss, stimulating regrowth, and improving hair density, with notable user satisfaction. No side effects were observed, and no participants were lost to follow-up or discontinued the protocol.

[0170] In accordance with the International Hair Science Association's Comparative Scoring Criteria, the clinical evaluation of the serum in Example 1 meets the five criteria established by the association: - effectiveness in hair regrowth, - the quality of the data collected, - safety and absence of side effects upon application, - the practicality of use, - Inclusivity, the serum can be used regardless of gender, skin phototype and hair type.

[0171] These results support the efficacy of the Serum in Example 1 and suggest that it can be recommended as an effective and safe solution for combating hair loss or for preserving hair health.

Claims

Demands

1. Hair composition for cutaneous and follicular penetration characterized in that it comprises: (A) 45% to 95% by weight of exosomes from plant stem cells, (B) 2% to 40% by weight of an extract of fruit of the palm of the genus Serenoa, (C) 1% to 15% by weight of an extract of hair roots culture of Ocimum Basilicum, (D) 0.05% to 10% by weight of Niacinamide, (E) 0.05% to 10% by weight of Rosemary essential oil, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

2. Composition according to claim 1, characterized in that it comprises: (A) 60% to 94% by weight, in particular 75% to 93% by weight, more particularly 85% to 92% by weight of exosomes derived from plant stem cells, (B) 3% to 30% by weight, in particular 4% to 20% by weight, more particularly 5% to 10% by weight of an extract of the fruit of the palm tree of the genus Serenoa, (C) 1% to 10% by weight, in particular 1.5% to 7% by weight, more particularly 1.5% to 4% by weight of a cultured extract of hairy roots of Ocimum Basilicum, (D) 0.1% to 8% by weight, in particular 0.2% to 5% by weight, more particularly 0.3% to 1% in weight of Niacinamide, (E) from 0.08% to 8% by weight, in particular from 0.1% to 5% by weight, more particularly from 0.15% to 1% by weight of Rosemary essential oil, the percentages by weight being expressed in relation to the sum of the weights of (A), (B), (C), (D) and (E).

3. Composition according to claim 1 or 2, characterized in that the exosomes derived from plant stem cells are selected from exosomes derived from stem cells of flowering plants of the Cucurbitaceae family, in particular of the genus Lagenaria, such as Lagenaria siceraria, of the genus Sicyos, such as Sicyos angulatus, or of the genus Benincasa, such as Benincasa hispida, or of the family Rosaceae, especially of the genus Malus, such as Malus domestica, of the genus Prunus, such as Prunus armeniaca or Prunus dulcis, or of the genus Rosa, such as Rosa gallica or Rosa canina.

4. Composition according to any one of claims 1 to 3, characterized in that it further comprises a vitamin, in particular selected from the B group such as: B1, B2, B5, B6, B7, B8, B12, a vitamin C, a vitamin E, or a mixture thereof.

5. Composition according to any one of claims 1 to 4, characterized in that it further comprises a humectant or moisturizing agent, in particular selected from: glycerin, aloe vera, soybean germ extract, glycosaminoglycan, retinyl palmitate, ferric sulfate or a mixture thereof.

6. Composition according to any one of claims 1 to 5, characterized in that it further comprises a conditioner, in particular selected from: hydrolyzed keratin, coconut extract, sunflower seed oil, or a vasodilator, such as Mentha piperita essential oil.

7. Composition according to any one of claims 1 to 6, characterized in that it further comprises a preservative, an antiseptic, an antimicrobial, an antioxidant and / or a pH regulator.

8. Composition according to any one of claims 1 to 7, characterized in that it further comprises an emulsifying agent, a surfactant, a gelling agent, a viscosity control agent, a bulking agent, a coloring agent and / or a perfumer.

9. Composition according to any one of claims 1 to 8, characterized in that it is in the form of a lotion, serum, shampoo, conditioner, dry shampoo, gel, wax, lacquer, spray, mousse, paste, cream, powder, oil, mask, treatment, toner, said composition possibly further comprising an aqueous, alcoholic or hydroalcoholic solvent.

10. Composition according to any one of claims 1 to 9, for its use in the prevention or reduction of hair loss.

11. Composition for its use according to claim 10, characterized in that the composition is applied to the scalp, and the application is optionally followed by a massage or friction of the scalp.

12. Composition for its use according to claim 11, characterized in that the composition is further rinsed.

13. Cosmetic use of the composition according to any one of claims 1 to 9 to increase hair density, or increase hair diameter.

14. A cosmetic hair treatment method comprising applying the composition according to any one of claims 1 to 9 to the scalp, optionally followed by a scalp massage or friction.

15. A process according to claim 14 further comprising a step of rinsing the composition.

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