Liver-clearing, restlessness-relieving and nerve-soothing cream formula and preparation method thereof

Abstract

The invention discloses a liver-clearing, restlessness-relieving and nerve-soothing cream formula and a preparation method thereof. The invention discloses a liver-clearing, restlessness-relieving and nerve-calming ointment The traditional Chinese medicine composition comprises the following components in parts by weight: 5-15 parts of pericarpium citri reticulatae, 15-45 parts of poria cocos, 10-20 parts of rhizoma pinellinae praeparata, 5-15 parts of fructus aurantii immaturus, 10-20 parts of fried semen ziziphi spinosae, 3-10 parts of baked ginger, 5-15 parts of schisandra chinensis, 5-20 parts of angelica sinensis, 5-20 parts of raw rhizoma atractylodis macrocephalae, 3-15 parts of cortex magnoliae officinalis, 3-10 parts of honey-fried licorice root, 5-15 parts of poria with hostwood, 5-15 parts of cassia twig, 5-15 parts of pericarpium citri reticulatae viride, 5-20 parts of radix bupleuri, 10-20 parts of cortex moutan, 3-10 parts of fructus gardeniae, 5-10 parts of mint, 5-20 parts of radix paeoniae alba and 100 to 200 parts of honey. The invention also provides a preparation method of the liver-clearing, restlessness-relieving and nerve-calming ointment. The traditional Chinese medicine composition is used for treating chronic insomnia caused by liver depression transforming into fire and damp obstruction and qi stagnation, and has the effects of soothing liver-qi stagnation, clearing away the heart-fire, promoting qi circulation, reducing phlegm, soothing the nerves, stabilizing the mind and the like.

Description

Technical Field

[0001] The invention relates to a traditional Chinese medicine paste prescription for clearing the liver, eliminating restlessness and calming the mind, and in particular to a paste prescription for clearing the liver, eliminating restlessness and calming the mind and a preparation method thereof. Background Art

[0002] Chronic insomnia refers to long-term difficulty sleeping that impairs daytime function. It is characterized by difficulty falling asleep, waking easily, waking early, and impaired sleep maintenance. According to statistics, insomnia is the most common sleep disorder worldwide, characterized by sleep disturbances, difficulty falling asleep, and frequent awakenings. Insomnia has become a widespread problem that seriously impacts public health. Its harmful effects not only lead to daytime fatigue, poor concentration, and decreased cognitive function, significantly reducing work and study efficiency and quality of life, but are also a significant risk factor for inducing or exacerbating anxiety, depression, cardiovascular disease, and other conditions.

[0003] Modern medical treatments primarily rely on sedative hypnotics (such as zopiclone, alprazolam, and diazepam) and antidepressants. While these can provide short-term relief for insomnia, long-term use carries significant drawbacks, including drug dependence, tolerance, next-day residual effects (such as drowsiness and dizziness), and cognitive impairment. In contrast, Traditional Chinese Medicine (TCM) demonstrates unique value through its synergistic compatibility, holistic conditioning, and multi-component, multi-target therapeutic approaches. Furthermore, the sustained-release properties of paste formulations enable steady and sustained drug release, effectively avoiding the dependency and withdrawal reactions common with Western medicine.

[0004] The inventor has found in previous clinical practice research that there is a type of insomnia patients with liver depression turning into fire and phlegm blocking qi stagnation, which is more common in people who like to drink beverages or have irregular meals due to high work pressure. This is because the high sugar in beverages belongs to phlegm and dampness in traditional Chinese medicine, which will stagnate the spleen and earth. People with high work pressure and irregular meals will disrupt the physiological rhythm of the spleen and stomach, damage the gastric mucosa, and induce gastritis. The spleen and stomach are weak and dampness is prevalent, and the spleen is often weak and damp. In addition, in modern society with a developed economy, a fast pace of life and work, and high work intensity, people generally think and worry a lot. People with excessive liver qi disharmony are particularly common. Liver qi disharmony invades the spleen and earth, aggravating the weakness of the spleen and earth, causing the spleen to fail to transport and transform, and normal food cannot be absorbed as nutrients, but instead becomes pathological metabolic products. Dampness obstruction makes people difficult to fall asleep, have shallow sleep and wake up easily, have many dreams and wake up early, leading to daytime fatigue, decreased memory and attention, affecting life and work. It is necessary to create a safe, effective, easy-to-use Chinese medicine preparation suitable for long-term use with conditioning properties for such people. Summary of the Invention

[0005] The first object of the present invention is to provide a liver-clearing, restlessness-removing and mind-calming paste for treating chronic insomnia of the liver depression-fire-transforming syndrome and dampness-blocking qi stagnation syndrome, which has the effects of soothing the liver and relieving depression, clearing the heart and reducing fire, promoting qi and resolving phlegm, and calming the mind and stabilizing the spirit.

[0006] The second object of the present invention is to provide a method for preparing the above-mentioned liver-clearing, vexation-removing and mind-calming paste.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] A liver-clearing, restlessness-relieving and mind-calming paste recipe comprises the following components by weight: 5-15 parts of dried tangerine peel, 15-45 parts of poria cocos, 10-20 parts of pinellia ternata, 5-15 parts of immature bitter orange, 10-20 parts of stir-fried spinach seeds, 3-10 parts of roasted ginger, 5-15 parts of schisandra chinensis, 5-20 parts of angelica sinensis, 5-20 parts of raw atractylodes macrocephala, 3-15 parts of magnolia bark, 3-10 parts of roasted liquorice root, 5-15 parts of poria, 5-15 parts of cinnamon twig, 5-15 parts of green peel, 5-20 parts of bupleurum, 10-20 parts of moutan bark, 3-10 parts of gardenia, 5-10 parts of mint, 5-20 parts of white peony root, 10-50 parts of raw rehmannia root, and 100-200 parts of honey.

[0009] Preferably, the following components are included in parts by weight: 5 parts of dried tangerine peel, 15 parts of Poria cocos, 10 parts of French pinellia, 5 parts of immature bitter orange, 10 parts of stir-fried spinach seeds, 3 parts of roasted ginger, 5 parts of Schisandra chinensis, 5 parts of angelica, 5 parts of raw Atractylodes macrocephala, 3 parts of Magnolia officinalis, 3 parts of roasted licorice, 5 parts of Poria, 5 parts of cinnamon twig, 5 parts of green peel, 5 parts of bupleurum, 10 parts of moutan bark, 3 parts of gardenia, 5 parts of mint, 5 parts of white peony root, 10 parts of raw Rehmannia root, and 100 parts of honey.

[0010] Preferably, the following components are included in parts by weight: 7.5 parts of dried tangerine peel, 22.5 parts of Poria cocos, 11.5 parts of French pinellia, 7.5 parts of immature bitter orange, 11.5 parts of stir-fried spinach seeds, 5 parts of roasted ginger, 7.5 parts of Schisandra chinensis, 9.5 parts of angelica sinensis, 9.5 parts of raw Atractylodes macrocephala, 7 parts of Magnolia officinalis, 5 parts of roasted liquorice, 7.5 parts of Poria, 7.5 parts of cinnamon twig, 7.5 parts of green peel, 9.5 parts of bupleurum, 11.5 parts of moutan bark, 5 parts of gardenia, 5.5 parts of mint, 9.5 parts of white peony root, 22.5 parts of raw Rehmannia root, and 115 parts of honey.

[0011] Preferably, the following components are included in parts by weight: 10 parts of dried tangerine peel, 30 parts of Poria cocos, 15 parts of French pinellia, 10 parts of immature bitter orange, 15 parts of stir-fried spinach seeds, 6.5 parts of roasted ginger, 10 parts of Schisandra chinensis, 12.5 parts of angelica sinensis, 12.5 parts of raw Atractylodes macrocephala, 9 parts of Magnolia officinalis, 6.5 parts of roasted liquorice, 10 parts of Poria, 10 parts of cinnamon twig, 10 parts of green peel, 12.5 parts of bupleurum, 15 parts of moutan bark, 6.5 parts of gardenia, 7.5 parts of mint, 12.5 parts of white peony root, 30 parts of raw Rehmannia root, and 150 parts of honey.

[0012] Preferably, the following components are included in parts by weight: 12.5 parts of dried tangerine peel, 37.5 parts of Poria cocos, 19 parts of French pinellia, 12.5 parts of immature bitter orange, 19 parts of stir-fried spinach seeds, 8 parts of roasted ginger, 12.5 parts of schisandra chinensis, 15.5 parts of angelica sinensis, 15.5 parts of raw white atractylodes, 11.5 parts of Magnolia officinalis, 8 parts of roasted liquorice, 12.5 parts of poria, 12.5 parts of cinnamon twig, 12.5 parts of green peel, 15.5 parts of bupleurum, 19 parts of moutan bark, 8 parts of gardenia, 9.5 parts of mint, 15.5 parts of white peony root, 37.5 parts of raw rehmannia, and 190 parts of honey.

[0013] Preferably, the following components are included in parts by weight: 15 parts of dried tangerine peel, 45 parts of Poria cocos, 20 parts of French pinellia, 15 parts of immature bitter orange, 20 parts of stir-fried spinach seeds, 10 parts of roasted ginger, 15 parts of Schisandra chinensis, 20 parts of angelica sinensis, 20 parts of raw Atractylodes macrocephala, 15 parts of Magnolia officinalis, 10 parts of roasted licorice, 15 parts of Poria, 15 parts of cinnamon twig, 15 parts of green peel, 20 parts of bupleurum, 20 parts of moutan bark, 10 parts of gardenia, 10 parts of mint, 20 parts of white peony root, 50 parts of raw rehmannia, and 200 parts of honey.

[0014] A preparation method of a liver-clearing, restlessness-relief and mind-calming paste comprises the following steps:

[0015] 7.1 Weigh tangerine peel, Poria cocos, French pinellia, immature bitter orange, roasted ginger, Schisandra chinensis, Chinese angelica, raw white atractylodes, Magnolia bark, roasted liquorice root, Poria, cinnamon twig, green peel, bupleurum, moutan bark, gardenia, mint, white peony root, raw rehmannia root together and soak for 7-9 hours;

[0016] 7.2 Stir-fry the spinach seeds to extract the fat-soluble tranquilizing ingredients. Then, use water to extract the water-soluble ingredients from the filtered medicinal residue for later use.

[0017] 7.3 After soaking the Chinese medicinal materials in step 7.1, place them in a pot and simmer for two times, each time for 1.3-1.7 hours. Add mint 7-13 minutes before the end of the second decocting.

[0018] 7.4 Add the water-soluble ingredients obtained in step 7.2 to the medicinal liquid obtained after decoction in step 7.3, and then filter it 2-3 times. Concentrate the filtrate until it becomes a clear paste in the shape of honeycomb balls. Reduce the heat and add the melted honey. Keep stirring and control the temperature at 70-80℃ until the paste is formed.

[0019] Preferably, the Poria cocos is subjected to vacuum freeze-drying technology before step 7.1 to retain its active ingredients;

[0020] In step 7.1, put dried tangerine peel, Poria cocos, Pinellia ternata, Citrus aurantium, Rhizoma Zingiberis, Schisandrae Chinensis, Chinese Angelicae Sinensis, Atractylodes macrocephala, Magnolia officinalis, Roasted Licorice, Poria, Cinnamon twig, Citrus aurantium, Bupleurum chinense, Cortex Moutan, Gardenia jasminoides, Mentha, White Peony Root, and Rehmannia glutinosa into a cloth bag and soak it in purified water 6-8 times the weight of the medicinal materials in this step for 8 hours;

[0021] In step 7.2, a predetermined amount of stir-fried spinach seeds is weighed and subjected to a component extraction process using a combined alcohol extraction-water extraction process, namely, based on different solvent solubilities, first using an ethanol-water solution with a volume ratio of water to ethanol of 70% by volume at 70-80° C., wherein the weight ratio of the stir-fried spinach seeds to the ethanol-water solution is 1:8-10, reflux for 1.9-2.1 hours, and repeated twice, and then concentrated under reduced pressure at 55° C.-65° C. to remove alcohol to obtain the fat-soluble sedative component of the stir-fried spinach seeds; the spinach seeds residue after the fat-soluble sedative component is extracted is filtered and separated, and washed thoroughly with pure water until no ethanol residue is left, and then the spinach seeds residue is decocted with 6-8 times the weight of the spinach seeds residue at normal pressure of 90±2° C. for 1.4-1.6 hours to obtain the water-soluble component of the residue;

[0022] In step 7.3, simmer for 1.5 hours twice, adding mint 10 minutes before the end of the second simmering;

[0023] In step 7.4, filter 2-3 times with a sieve of 80 to 100 mesh. The paste is collected by using the standard of water droplets forming beads and hanging flags. After the paste is collected, it is packaged into sterilized glass bottles while hot and sterilized with high-pressure steam at 115℃-125℃ for 20-30 minutes. After the paste is cooled, it can be sealed.

[0024] The liver-clearing, restlessness-eliminating and mind-calming paste of the present invention can be used to treat chronic insomnia of liver depression transforming into fire, dampness blocking and qi stagnation syndrome, and the like.

[0025] The beneficial effects of the present invention are as follows:

[0026] The Chinese medicinal paste of the present invention is suitable for the combination of medicinal materials, conforms to the theories of traditional Chinese medicine and modern medicine, and has the effects of soothing the liver and relieving depression, clearing the heart and reducing fire, promoting qi and resolving phlegm, and calming the mind. In the theory of traditional Chinese medicine, liver depression and fire transformation, emotional imbalance, and emotional stagnation caused by imbalance of yin and yang and lack of nourishment of the mind lead to liver qi stagnation, which turns into fire and disturbs the mind, and ultimately manifests as difficulty falling asleep, irritability and dreaminess. Therefore, in the treatment of this syndrome, it is necessary to regulate the qi mechanism and combine clearing fire and nourishing yin to calm the mind and take into account the spleen-strengthening, promoting qi and resolving phlegm. A comprehensive treatment targeting the complexity of the pathogenesis is required to achieve both symptomatic and root cause treatment, which should have a better effect.

[0027] The paste prescription composed of the above-mentioned Chinese medicine combination of the present invention can soothe the liver, clear away heat, calm the mind and stabilize the spirit, and at the same time strengthen the spleen, promote qi and eliminate phlegm and dampness, and treat various insomnia caused by liver depression, qi stagnation, fire transformation and spleen earth generation of phlegm and dampness. Compared with the single Chinese patent medicines sold on the market that soothe the liver and clear heat, strengthen the spleen and replenish blood to nourish the heart, or connect the heart and kidneys, this combination takes into account the pathogenesis of spleen deficiency and dampness obstructing the mind in Lingnan area of ​​Guangdong. It uses tangerine peel, Poria, Pinellia ternata, Atractylodes macrocephala to strengthen the spleen and stomach and eliminate phlegm and dampness. "The spleen is the source of phlegm", which reduces the interference of phlegm and dampness on the mind and makes it difficult to fall asleep. Bupleurum and mint soothe liver depression and smooth the qi. Citrus aurantium, Magnolia officinalis, and Citrus aurantium promote qi, break up qi and relieve depression, taking the idea that "qi moves when water moves". Gardenia, Cortex Moutan, and Rehmannia root clear heat, nourish yin and relieve restlessness. It is supplemented with stir-fried Ziziphus jujuba seeds and Poria cocos to calm the mind. Schisandra chinensis helps to calm the mind and restrain yin. Cinnamon twig and dried ginger warm and prevent coldness. Angelica sinensis and white peony root nourish blood and soften the liver. Roasted licorice harmonizes all the medicines. The whole prescription has the effects of soothing the liver, promoting qi and relieving depression, clearing heat and nourishing yin, strengthening the spleen and resolving phlegm, nourishing blood and softening the liver and soothing the mind. It is mainly used to treat insomnia, anxiety, depression, etc. caused by various types of qi stagnation turning into fire, spleen deficiency and dampness obstruction, and restlessness. For example, sleep abnormalities caused by emotional diseases such as menopausal syndrome, anxiety, and depression. Symptoms include difficulty falling asleep, early awakening, easy awakening, difficulty continuing to sleep after waking up, daytime restlessness, impatience, irritability, dry mouth, hot flashes and sweating, poor appetite, fullness and discomfort in the upper abdomen or ribs, red tongue with little coating, and deep, thin or stringy pulse. It can shorten the time to fall asleep, improve sleep quality, and improve daytime restlessness, irritability, and sleepiness.

[0028] In addition, the Chinese medicine composition of the present invention has a safe and reliable formulation of Chinese medicine raw materials, low technical risk, broad market demand, and obvious economic and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0030] Figure 1a Schematic diagram of the righting experiment results of the sleep latency of mice in the present invention;

[0031] Figure 1b Schematic diagram of the righting experiment results of the sleep duration of each mouse in the present invention;

[0032] Figure 2a This is a schematic diagram of the open field test analysis of the total distance each mouse enters the central area after drug administration in the present invention;

[0033] Figure 2b This is a schematic diagram of the open field test analysis of the number of times each mouse entered the central area after drug administration in the present invention;

[0034] Figure 2c A schematic diagram of the open field test analysis of the time taken by each mouse to enter the central area after drug administration in the present invention; and

[0035] Figure 3 Schematic diagram of the analysis of the experimental results of novel object recognition by each mouse in the present invention.

[0036] Explanation of the accompanying symbols: * represents P<0.1, ** represents P<0.01, *** represents P<0.001, and **** represents P<0.0001. DETAILED DESCRIPTION

[0037] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0038] A prescription for clearing the liver, relieving restlessness and tranquilizing the mind paste comprises the following components in parts by weight: 5-15 parts of dried tangerine peel, 15-45 parts of poria, 10-20 parts of pinellia ternata, 5-15 parts of immature bitter orange, 10-20 parts of stir-fried spinach seeds, 3-10 parts of roasted ginger, 5-15 parts of schisandra chinensis, 5-20 parts of raw atractylodes macrocephala, 3-15 parts of magnolia bark, 5-20 parts of roasted liquorice root, 5-15 parts of poria, 5-15 parts of cinnamon twig, 5-15 parts of green peel, 5-20 parts of bupleurum, 10-20 parts of moutan bark, 3-10 parts of gardenia, 5-10 parts of mint, 5-20 parts of white peony root, 10-50 parts of raw rehmannia root, and 100-200 parts of honey.

[0039] A preparation method of a liver-clearing, restlessness-relief and mind-calming paste comprises the following steps:

[0040] 7.1 Weigh tangerine peel, Poria cocos, Pinellia ternata, Citrus aurantium, Rhizoma Zingiberis, Schisandrae Chinensis, Chinese Angelicae Sinensis, Atractylodes macrocephala, Magnolia officinalis, Roasted Licorice, Poria cocos, Cinnamon twig, Citrus aurantium, Bupleurum chinense, Cortex Moutan, Gardenia jasminoides, Menthol, White Peony Root, and Rehmannia glutinosa together in proportion by weight and soak for 7-9 hours;

[0041] 7.2 Stir-fry the spinach seeds to extract the fat-soluble tranquilizing ingredients. Then, use water to extract the water-soluble ingredients from the filtered medicinal residue for later use.

[0042] 7.3 After soaking the Chinese medicinal materials in step 7.1, place them in a pot and simmer for two times, each time for 1.3-1.7 hours. Add mint 7-13 minutes before the end of the second decocting.

[0043] 7.4 Add the water-soluble ingredients obtained in step 7.2 to the medicinal liquid obtained after decoction in step 7.3, and then filter it 2-3 times. Concentrate the filtrate until it becomes a clear paste in the shape of honeycomb balls. Reduce the heat and add the melted honey. Keep stirring and control the temperature at 70-80℃ until the paste is formed.

[0044] Preferably, the Poria cocos is subjected to vacuum freeze drying technology before step 7.1 to retain its active ingredients, wherein the vacuum freeze drying technology is used to dehydrate the medicinal material by low-temperature freezing and vacuum sublimation, thereby maximally retaining the active ingredients of the traditional Chinese medicine;

[0045] In step 7.1, put dried tangerine peel, Poria cocos, Pinellia ternata, Citrus aurantium, Rhizoma Zingiberis, Schisandrae Chinensis, Chinese Angelicae Sinensis, Atractylodes macrocephala, Magnolia officinalis, Roasted Licorice, Poria, Cinnamon twig, Citrus aurantium, Bupleurum chinense, Cortex Moutan, Gardenia jasminoides, Mentha, White Peony Root, and Rehmannia glutinosa into a cloth bag and soak it in purified water 6-8 times the weight of the medicinal materials in this step for 8 hours;

[0046] In step 7.2, a predetermined amount of stir-fried spinach seeds is weighed and subjected to a component extraction process using an alcohol extraction-water extraction combined process. That is, based on the different solubility of the solvent, an ethanol-water solution having a volume ratio of water to ethanol of 70% is first used for heating and reflux extraction at 70-80° C., wherein the weight ratio of the stir-fried spinach seeds to the ethanol-water solution is 1:8-10, and the process is refluxed for 1.9-2.1 hours, for example, 1.9, 2.0, or 2.1 hours, repeated twice, and then concentrated under reduced pressure at 55° C.-65° C. to remove the alcohol to obtain the fat-soluble sedative component of the stir-fried spinach seeds. , for example: 55°C or 60°C or 65°C; after extracting the fat-soluble tranquilizing component, the Chinese jujube seed residue is filtered and separated and fully washed with pure water until no ethanol residue is left, and then the Chinese jujube seed residue is decocted with 6-8 times the weight of pure water of the Chinese jujube seed residue at normal pressure and 90±2°C for 1.4-1.6 hours to obtain the water-soluble component of the residue, for example: 1.4 or 1.5 or 1.6 hours, wherein the alcohol extraction-water extraction combined process is adopted, and the effective components of different polarities in the traditional Chinese medicine can be extracted by the different solubility of the solvent, thereby achieving efficient and comprehensive extraction;

[0047] In step 7.3, simmer for 1.5 hours twice, adding mint 10 minutes before the end of the second simmering;

[0048] In step 7.4, filter 2-3 times with a sieve of 80 to 100 mesh. The paste is collected by using the standard of water droplets forming beads and hanging flags. After the paste is collected, it is packaged into sterilized glass bottles while hot and sterilized with high-pressure steam at 115℃-125℃ for 20-30 minutes. After the paste is cooled, it can be sealed.

[0049] In addition, the quality control standards are as follows (relative density: tonic pastes ≥1.40 (25°C): heat-clearing pastes ≥1.35; microbial limits: total colony count ≤100CFU / g, mold and yeast ≤10CFU / g, no pathogenic bacteria shall be detected; heavy metals and pesticide residues: lead ≤5mg / kg, cadmium ≤0.3mg / kg, pesticide residues shall comply with the requirements of the "Quality Management Standards for the Production of Chinese Medicinal Materials"). After passing the standards, they can be stored.

[0050] The liver-clearing, restlessness-eliminating and mind-calming paste of the present invention can be used to treat chronic insomnia of liver depression transforming into fire, dampness blocking and qi stagnation syndrome, and the like.

[0051] Among them, the liver-clearing, restlessness-relief and tranquilizing ointment prescription of the present invention has 5 preferred formulas, and the preferred formulas and preparation methods thereof are as follows:

[0052] Preferred formula 1, a liver-clearing, irritability-removing and tranquilizing paste prescription, weighed by weight: 5 parts of dried tangerine peel, 15 parts of Poria cocos, 10 parts of French Pinellia, 5 parts of Citrus aurantium, 10 parts of stir-fried Ziziphus jujuba seeds, 3 parts of roasted ginger, 5 parts of Schisandra chinensis, 5 parts of Angelica sinensis, 5 parts of raw Atractylodes macrocephala, 3 parts of Magnolia officinalis, 3 parts of roasted Licorice root, 5 parts of Poria, 5 parts of cinnamon twig, 5 parts of Citrus aurantium, 5 parts of Bupleurum chinense, 10 parts of Cortex Moutan, 3 parts of Gardenia jasminoides, 5 parts of mint, 5 parts of white peony root, 10 parts of raw Rehmannia root, and 100 parts of honey.

[0053] The preparation method is as follows:

[0054] Weigh by weight: 5 parts of dried tangerine peel, 15 parts of Poria cocos, 10 parts of French pinellia, 5 parts of immature bitter orange, 10 parts of stir-fried spinach seeds, 3 parts of roasted ginger, 5 parts of Schisandra chinensis, 5 parts of angelica, 5 parts of raw Atractylodes macrocephala, 3 parts of Magnolia officinalis, 3 parts of roasted liquorice, 5 parts of Poria, 5 parts of cinnamon twig, 5 parts of green peel, 5 parts of bupleurum, 10 parts of moutan bark, 3 parts of gardenia, 5 parts of mint, 5 parts of white peony root, 10 parts of raw rehmannia, and 100 parts of honey.

[0055] Weigh the remaining herbs, except for the stir-fried sour jujube kernels, mint, and honey, and place them in a cloth bag. Add 6-8 times the weight of the herbs to a bag and soak for 8 hours. Extract the stir-fried sour jujube kernels with 70% alcohol and then with water. Bag the mint separately for later use.

[0056] After soaking, decoct the herbs twice (1.5 hours each time, simmering), adding the mint 10 minutes before the end of the second decoction. Filter the combined liquid through an 80- to 100-mesh sieve three times, concentrating it into a honeycomb-like paste. Simmer the mixture over low heat and add melted honey (control the temperature below 80°C). The paste should form beads or resemble flags.

[0057] While hot, divide the mixture into sterilized bottles, sterilize under high pressure at 121℃ for 30 minutes, cool and seal for storage.

[0058] Preferred formula 2, a liver-clearing, irritability-removing and tranquilizing paste prescription, weighed by weight: 7.5 parts of dried tangerine peel, 22.5 parts of Poria cocos, 11.5 parts of French pinellia, 7.5 parts of immature bitter orange, 11.5 parts of stir-fried spinach seeds, 5 parts of roasted ginger, 7.5 parts of Schisandra chinensis, 9.5 parts of angelica sinensis, 9.5 parts of raw Atractylodes macrocephala, 7 parts, 9.5 parts of Magnolia officinalis, 5 parts of roasted liquorice, 7.5 parts of Poria, 7.5 parts of cinnamon twig, 7.5 parts of green peel, 9.5 parts of bupleurum, 11.5 parts of moutan bark, 5 parts of gardenia, 5.5 parts of mint, 9.5 parts of white peony root, 22.5 parts of raw Rehmannia root, and 115 parts of honey.

[0059] The preparation method is as follows:

[0060] Weigh by weight: 7.5 parts of dried tangerine peel, 22.5 parts of Poria cocos, 11.5 parts of French pinellia, 7.5 parts of immature bitter orange, 11.5 parts of stir-fried spinach seeds, 5 parts of roasted ginger, 7.5 parts of Schisandra chinensis, 9.5 parts of angelica, 9.5 parts of raw Atractylodes macrocephala, 7 parts of Magnolia officinalis, 5 parts of roasted liquorice, 7.5 parts of Poria, 7.5 parts of cinnamon twig, 7.5 parts of green peel, 9.5 parts of bupleurum, 11.5 parts of moutan bark, 5 parts of gardenia, 5.5 parts of mint, 9.5 parts of white peony root, 22.5 parts of raw rehmannia, and 115 parts of honey.

[0061] Weigh the remaining herbs, except for the stir-fried sour jujube kernels, mint, and honey, and place them in a cloth bag. Add 6-8 times the weight of the herbs to a bag and soak for 8 hours. Extract the stir-fried sour jujube kernels with 70% alcohol and then with water. Bag the mint separately for later use.

[0062] After soaking, decoct the herbs twice (1.5 hours each time, simmering), adding the mint 10 minutes before the end of the second decoction. Filter the combined liquid through an 80- to 100-mesh sieve three times, concentrating it into a honeycomb-like paste. Simmer the mixture over low heat and add melted honey (control the temperature below 80°C). The paste should form beads or resemble flags.

[0063] While hot, divide the mixture into sterilized bottles, sterilize under high pressure at 121℃ for 30 minutes, cool and seal for storage.

[0064] Preferred formula 3, a liver-clearing, irritability-removing and tranquilizing paste prescription, weighed by weight: 10 parts of dried tangerine peel, 30 parts of Poria cocos, 15 parts of French Pinellia, 10 parts of Citrus aurantium, 15 parts of stir-fried Ziziphus jujuba seeds, 6.5 parts of roasted ginger, 10 parts of Schisandra chinensis, 12.5 parts of Angelica sinensis, 12.5 parts of raw Atractylodes macrocephala, 9 parts of Magnolia officinalis, 6.5 parts of roasted Licorice root, 10 parts of Poria, 10 parts of cinnamon twig, 10 parts of Citrus aurantium, 12.5 parts of Bupleurum chinense, 15 parts of Cortex Moutan, 6.5 parts of Gardenia jasminoides, 7.5 parts of mint, 12.5 parts of white peony root, 30 parts of Rehmannia glutinosa, and 150 parts of honey.

[0065] The preparation method is as follows:

[0066] Weigh by weight: 10 parts of dried tangerine peel, 30 parts of Poria cocos, 15 parts of French pinellia, 10 parts of immature bitter orange, 15 parts of stir-fried spinach seeds, 6.5 parts of roasted ginger, 10 parts of Schisandra chinensis, 12.5 parts of angelica sinensis, 12.5 parts of raw Atractylodes macrocephala, 9 parts of Magnolia officinalis, 6.5 parts of roasted liquorice, 10 parts of Poria, 10 parts of cinnamon twig, 10 parts of green peel, 12.5 parts of bupleurum, 15 parts of moutan bark, 6.5 parts of gardenia, 7.5 parts of mint, 12.5 parts of white peony root, 30 parts of raw rehmannia, and 150 parts of honey.

[0067] Weigh the remaining herbs, except for the stir-fried sour jujube kernels, mint, and honey, and place them in a cloth bag. Add 6-8 times the weight of the herbs to a bag and soak for 8 hours. Extract the stir-fried sour jujube kernels with 70% alcohol and then with water. Bag the mint separately for later use.

[0068] After soaking, decoct the herbs twice (1.5 hours each time, simmering), adding the mint 10 minutes before the end of the second decoction. Filter the combined liquid through an 80- to 100-mesh sieve three times, concentrating it into a honeycomb-like paste. Simmer the mixture over low heat and add melted honey (control the temperature below 80°C). The paste should form beads or resemble flags.

[0069] While hot, divide the mixture into sterilized bottles, sterilize under high pressure at 121℃ for 30 minutes, cool and seal for storage.

[0070] Preferred formula 4, a liver-clearing, restlessness-relief and tranquilizing paste prescription, weighed by weight: 12.5 parts of dried tangerine peel, 37.5 parts of Poria cocos, 19 parts of French Pinellia, 12.5 parts of Citrus aurantium, 19 parts of stir-fried Ziziphus jujuba seeds, 8 parts of roasted ginger, 12.5 parts of Schisandra chinensis, 15.5 parts of Angelica sinensis, 15.5 parts of raw Atractylodes macrocephala, 11.5 parts of Magnolia officinalis, 8 parts of roasted Licorice root, 12.5 parts of Poria, 12.5 parts of cinnamon twig, 12.5 parts of Citrus aurantium, 15.5 parts of Bupleurum chinense, 19 parts of Cortex Moutan, 8 parts of Gardenia jasminoides, 9.5 parts of mint, 15.5 parts of white peony root, 37.5 parts of Rehmannia glutinosa, and 190 parts of honey.

[0071] The preparation method is as follows:

[0072] Weigh by weight: 12.5 parts of dried tangerine peel, 37.5 parts of Poria cocos, 19 parts of French pinellia, 12.5 parts of immature bitter orange, 19 parts of stir-fried spinach seeds, 8 parts of roasted ginger, 12.5 parts of schisandra chinensis, 15.5 parts of angelica sinensis, 15.5 parts of raw white atractylodes, 11.5 parts of Magnolia officinalis, 8 parts of roasted liquorice, 12.5 parts of poria, 12.5 parts of cinnamon twig, 12.5 parts of green peel, 15.5 parts of bupleurum, 19 parts of moutan bark, 8 parts of gardenia, 9.5 parts of mint, 15.5 parts of white peony root, 37.5 parts of raw rehmannia, and 190 parts of honey.

[0073] Weigh the remaining herbs, except for the stir-fried sour jujube kernels, mint, and honey, and place them in a cloth bag. Add 6-8 times the weight of the herbs to a bag and soak for 8 hours. Extract the stir-fried sour jujube kernels with 70% alcohol and then with water. Bag the mint separately for later use.

[0074] After soaking, decoct the herbs twice (1.5 hours each time, simmering), adding the mint 10 minutes before the end of the second decoction. Filter the combined liquid through an 80- to 100-mesh sieve three times, concentrating it into a honeycomb-like paste. Simmer the mixture over low heat and add melted honey (control the temperature below 80°C). The paste should form beads or resemble flags.

[0075] While hot, divide the mixture into sterilized bottles, sterilize under high pressure at 121℃ for 30 minutes, cool and seal for storage.

[0076] Preferred formula 5, a liver-clearing, irritability-removing and tranquilizing paste prescription, weighed by weight: 15 parts of dried tangerine peel, 45 parts of Poria cocos, 20 parts of French Pinellia, 15 parts of Citrus aurantium, 20 parts of stir-fried Ziziphus jujuba seeds, 10 parts of roasted ginger, 15 parts of Schisandra chinensis, 20 parts of Angelica sinensis, 20 parts of raw Atractylodes macrocephala, 15 parts of Magnolia officinalis, 10 parts of roasted Licorice root, 15 parts of Poria, 15 parts of cinnamon twig, 15 parts of Citrus aurantium, 20 parts of Bupleurum chinense, 20 parts of Cortex Moutan, 10 parts of Gardenia jasminoides, 10 parts of mint, 20 parts of white peony root, 50 parts of raw Rehmannia glutinosa, and 200 parts of honey.

[0077] The preparation method is as follows:

[0078] Weigh by weight: 15 parts of dried tangerine peel, 45 parts of Poria cocos, 20 parts of French pinellia, 15 parts of immature bitter orange, 20 parts of stir-fried spinach seeds, 10 parts of roasted ginger, 15 parts of Schisandra chinensis, 20 parts of angelica sinensis, 20 parts of raw Atractylodes macrocephala, 15 parts of Magnolia officinalis, 10 parts of roasted liquorice, 15 parts of Poria, 15 parts of cinnamon twig, 15 parts of green peel, 20 parts of bupleurum, 20 parts of moutan bark, 10 parts of gardenia, 10 parts of mint, 20 parts of white peony root, 50 parts of raw rehmannia, and 200 parts of honey.

[0079] Weigh the remaining herbs, except for the stir-fried sour jujube kernels, mint, and honey, and place them in a cloth bag. Add 6-8 times the weight of the herbs to a bag and soak for 8 hours. Extract the stir-fried sour jujube kernels with 70% alcohol and then with water. Bag the mint separately for later use.

[0080] After soaking, decoct the herbs twice (1.5 hours each time, simmering), adding the mint 10 minutes before the end of the second decoction. Filter the combined liquid through an 80- to 100-mesh sieve three times, concentrating it into a honeycomb-like paste. Simmer the mixture over low heat and add melted honey (control the temperature below 80°C). The paste should form beads or resemble flags.

[0081] While hot, divide the mixture into sterilized bottles, sterilize under high pressure at 121℃ for 30 minutes, cool and seal for storage.

[0082] The intervention and effects of the Qinggan Chufan Anshen ointment of the present invention on insomnia model mice are as follows:

[0083] 1 Materials and Methods

[0084] 1.1 Experimental Animals

[0085] Sixty healthy, SPF-grade, male C57BL / 6J mice (18 ± 2 g), aged 4-6 weeks, were housed at 24 ± 2°C and 50%-65% relative humidity. They were housed in cages with free access to food and water throughout the study, and maintained on a 12-hour light / dark cycle. The animals were acclimated for 7 days before the experiment. This study was approved by the Shenzhen Zhongxun Precision Medicine Research Institute.

[0086] 1.2 Investigational Drugs

[0087] The liver-clearing, vexation-removing and tranquilizing ointment of the present invention is prepared according to the method described in the preferred formula 3. Purified water is used to prepare a solution for administration.

[0088] Liver-clearing, restlessness-relief and tranquilizing paste: 10 parts of dried tangerine peel, 30 parts of Poria cocos, 15 parts of French pinellia, 10 parts of immature bitter orange, 15 parts of stir-fried spinach seeds, 6.5 parts of roasted ginger, 10 parts of schisandra chinensis, 12.5 parts of angelica sinensis, 12.5 parts of raw white atractylodes, 9 parts of Magnolia officinalis, 6.5 parts of roasted liquorice, 10 parts of poria, 10 parts of cinnamon twig, 10 parts of green peel, 12.5 parts of bupleurum, 15 parts of moutan bark, 6.5 parts of gardenia, 7.5 parts of mint, 12.5 parts of white peony root, 30 parts of raw rehmannia, and 150 parts of honey.

[0089] Weigh the remaining medicinal ingredients, except for the stir-fried Chinese jujube seeds, mint, and honey, in appropriate proportions and place them in a cloth bag. Soak the bag in purified water (6-8 times the weight of the ingredients) for 8 hours. Extract the stir-fried Chinese jujube seeds separately with 70% alcohol and then with water. Bag the mint separately for later use. After soaking, decoct the ingredients twice, adding the mint 10 minutes before the end of the second decoction. Filter the combined solution three times through an 80- to 100-mesh sieve and concentrate it into a honeycomb-like paste. Reduce heat to low and add the melted honey to dissolve the paste. Prepare the solution with purified water for administration.

[0090] 1.3 Grouping and modeling

[0091] Ten mice were randomly selected as the normal group, and the rest were model groups. The mice were randomly divided into the following five groups, with 10 mice in each group: model group, diazepam group, Qingganchufananshenan paste low-dose group, Qingganchufananshenan paste medium-dose group, and Qingganchufananshenan paste high-dose group.

[0092] This study used a modified chlorophenylalanine combined with chronic unpredictable mild stress model to establish an insomnia mouse model. Adaptive feeding was performed for 7 days. After the adaptation period, starting on the 8th day, all model group mice were exposed to chronic unpredictable mild stress stimulation for 6 weeks. One of the following stimulation methods was performed daily, including forced swimming, wet bedding, food and water deprivation, strong light stroboscopic stimulation, and light-dark reversal cycle. Starting from the 49th day, except for the normal group, all groups of mice were intraperitoneally injected with PCPA (350 mg / kg) every morning for 3 consecutive days. The normal group of mice was placed in the home cage and raised normally to ensure adequate sleep conditions. During the modeling period, all rats had free access to basic feed and distilled water. When the model mice showed typical characteristics such as curling up, lack of security, listlessness, withered fur, reduced food and water intake, and obvious stress response when grasping, it indicated that the model was successful.

[0093] 1.4 Administration

[0094] On the 52nd day, the mice in the Qingganchufananshenan paste low-dose group, Qingganchufananshenan paste medium-dose group, Qingganchufananshenan paste high-dose group, and diazepam group were given the designated doses for 7 days, and the control group and model group received an equal amount of normal saline by gavage.

[0095] 1.5 Behavioral testing

[0096] (1) Righting experiment

[0097] After modeling, mice in each group were intraperitoneally injected with 30 mg / kg sodium pentobarbital. Mice were placed on a flat surface with their abdomen facing upward and their bodies held vertically. The time from the time of intraperitoneal injection to the loss of the righting reflex was considered sleep latency. If the mouse turned over more than twice within 60 seconds, the righting reflex was considered recovered. The time from the loss of the righting reflex to the recovery of the righting reflex was considered sleep duration.

[0098] (2 Open field experiment

[0099] Place the mouse in the center square of an open box (the size of the open box is 100 cm long x 100 cm wide x 40 cm high), use a counter to manually count the number of squares each mouse crosses horizontally within 5 minutes, record the time and number of times the mouse enters the central area, and calculate the total distance the mouse travels.

[0100] (3) New object recognition experiment

[0101] Two objects, A and B, of different shapes and colors, were placed in a chamber, 25 cm apart. On the first day, mice were allowed to acclimate to the chamber for 20 minutes. The two objects were identical (both objects were A). One hour later, object A on the left side was replaced with object B, and the time the mice spent in contact with A and B within a 5-minute period was recorded. After 24 hours, object A on the right side was replaced with object B, and the time the mice spent in contact with A and B within a 5-minute period was recorded.

[0102] 1.6 Statistical methods

[0103] SPSS 23.0 statistical software was used for data processing, and one-way analysis of variance was used to compare data among the groups. P < 0.05 was considered statistically significant.

[0104] 2 Results

[0105] 2.1 Results of the Reversal Experiment

[0106] Figure 1a 、 1b The results of the reversal experiment showed that compared with the control group, the sleep latency time of the mice in the model group was prolonged and the sleep time was shortened, and the differences were statistically significant (P < 0.05). After taking the medicine, the latency time of the mice in the low-dose Qinggan Chufan Anshen Paste group, the medium-dose Qinggan Chufan Anshen Paste group, the high-dose Qinggan Chufan Anshen Paste group, and the diazepam group was significantly shortened, and the differences were statistically significant (P < 0.05); the sleep time was also significantly prolonged, and the differences were statistically significant (P < 0.05), indicating that the Qinggan Chufan Anshen Paste prescription has a sedative and tranquilizing effect.

[0107] 2.2 Open field test results

[0108] See Figure 2a 、 Figure 2b 、 Figure 2c The results of exercise showed that the total distance traveled, the number of times the mice entered the central area, and the time they stayed in the model group were significantly lower than those in the control group. The total distance traveled by the mice in the medium-dose Qinggan Chufan Anshen Paste group, the high-dose Qinggan Chufan Anshen Paste group, and the diazepam group was statistically significant (P < 0.05). The number of times the mice entered the central area in the medium-dose Qinggan Chufan Anshen Paste group and the high-dose Qinggan Chufan Anshen Paste group was statistically significant (P < 0.05); the time the mice spent in the central area in the high-dose Qinggan Chufan Anshen Paste group was statistically significant (P < 0.05). The number of times the mice in the low-dose Qinggan Chufan Anshen Paste group, the medium-dose Qinggan Chufan Anshen Paste group, and the diazepam group entered the central area was increased, but not statistically significant. Compared with the model group, the total distance traveled by the mice in the high-dose Qinggan Chufan Anshen Paste group, the number of times the mice entered the central area, and the time they stayed in the central area were all increased, indicating that the Qinggan Chufan Anshen Paste formula can increase the spontaneous activity of mice and relieve their anxiety.

[0109] 2.3 Experimental results of new object recognition

[0110] See Figure 3The results of the novel object recognition experiment showed that the novel object recognition index of the model group mice was significantly lower than that of the control group. However, the novel object recognition index of the low-dose Qinggan Chufan Anshen Paste group, the medium-dose Qinggan Chufan Anshen Paste group, and the diazepam group increased with statistical significance (P < 0.05). The novel object recognition index of the medium-dose Qinggan Chufan Anshen Paste group and the diazepam group increased compared to the model group, indicating that the Qinggan Chufan Anshen Paste prescription helps improve learning and recognition abilities.

[0111] Animal experiments have shown that the Qinggan Chufan Anshen paste of the present invention has the effects of soothing liver qi and relieving depression, clearing the heart and reducing internal heat, and calming the mind and stabilizing the spirit. When applied to insomnia model mice, it can calm the mice, significantly shorten the time it takes to fall asleep, prolong sleep duration, and alleviate cognitive impairment caused by insomnia, thereby improving cognitive function, alleviating anxiety symptoms, and relieving fatigue, with significant effects. Therefore, the Qinggan Chufan Anshen paste has the effects of soothing liver qi and relieving depression, clearing the heart and reducing internal heat, and calming the mind and stabilizing the spirit in insomnia mice.

[0112] In summary, the effects and advantages of the present invention are as follows:

[0113] (1) The present invention provides a paste formula for treating insomnia of the liver depression transforming into fire and damaging yin, spleen deficiency and dampness blocking qi stagnation type.

[0114] (2) The paste formula of the present invention has a unique effect: the main medicine is Bupleurum chinense to relieve liver qi and depression, Citrus aurantium to break qi and relieve depression, Gardenia jasminoides and Cortex moutan to clear liver fire, which are the main drugs. The auxiliary medicine is stir-fried Ziziphus jujuba seeds to nourish the heart and calm the mind, Poria cocos to calm the heart and calm palpitations, and Schisandra chinensis to astringe yin and calm the mind to help the main medicine to calm the mind. The auxiliary medicine system is comprehensive: Raw Rehmannia root nourishes yin and reduces fire, Angelica sinensis and White Peony root nourish blood and soften the liver to treat the root cause; Raw Atractylodes macrocephala and Poria cocos strengthen the spleen and eliminate dampness, Tangerine peel, Magnolia bark, Citrus aurantium, and Pinellia ternata regulate qi and eliminate phlegm; Roasted ginger warms and promotes the flow of qi, cinnamon twig warms and promotes the flow of meridians, and peppermint penetrates heat and relieves depression. The auxiliary medicine is roasted licorice to harmonize the other drugs, and honey moisturizes and corrects the taste, helps the paste to form, and harmonizes the medicinal properties. The whole formula works synergistically on multiple targets, namely, relieving liver depression and relieving depression, clearing away heat and reducing fire, nourishing yin and blood, strengthening the spleen and eliminating dampness, and calming the mind. It targets the pathogenesis of insomnia caused by liver depression transforming into fire, deficiency heat, and internal disturbance of phlegm and dampness, and can effectively improve symptoms such as difficulty falling asleep, shallow sleep and easy awakening, frequent dreams, irritability and palpitations.

[0115] (3) Animal experiments show that the liver-clearing, annoyance-removing and tranquilizing ointment of the present invention has the effects of significantly shortening the time to fall asleep, prolonging the sleep duration, improving cognitive function, improving anxiety symptoms, and relieving fatigue in insomnia model mice. It is precisely because the present invention has the effects of soothing the liver and relieving depression, clearing the heart and reducing fire, promoting qi and resolving phlegm, and calming the mind that the insomnia model mice have the effects of significantly shortening the time to fall asleep, prolonging the sleep duration, improving cognitive function, improving anxiety symptoms, and relieving fatigue, and the effect is significant.

[0116] (4) The paste formula of the present invention is highly safe, easy to take, does not require decocting, is easy to store and carry, is suitable for long-term use, and has the effect of conditioning the body.

[0117] The above describes the preferred embodiments of the present invention. It should be understood that the present invention is not limited to the above-mentioned specific embodiments, and the devices and structures that are not described in detail should be understood to be implemented in a common manner in the art; any technician familiar with the art can use the above-mentioned disclosed methods and technical contents to make many possible changes and modifications to the technical solutions of the present invention without departing from the scope of the technical solutions of the present invention, or modify them into equivalent embodiments of equivalent changes, which does not affect the essential content of the present invention. Therefore, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention that do not depart from the content of the technical solutions of the present invention are still within the scope of protection of the technical solutions of the present invention.

Claims

1. A liver-clearing, restlessness-relieving and mind-calming ointment prescription, characterized in that: The invention comprises the following components by weight: 5-15 parts of dried tangerine peel, 15-45 parts of poria cocos, 10-20 parts of pinellia ternata, 5-15 parts of immature bitter orange, 10-20 parts of stir-fried spinach seeds, 3-10 parts of roasted ginger, 5-15 parts of schisandra chinensis, 5-20 parts of raw atractylodes macrocephala, 3-15 parts of magnolia bark, 3-10 parts of roasted liquorice, 5-15 parts of poria, 5-15 parts of cinnamon twig, 5-15 parts of green peel, 5-20 parts of bupleurum, 10-20 parts of moutan bark, 3-10 parts of gardenia, 5-10 parts of mint, 5-20 parts of white peony root, 10-50 parts of raw rehmannia root, and 100-200 parts of honey.

2. A liver-clearing, restlessness-relief and mind-calming paste according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 5 parts of dried tangerine peel, 15 parts of poria cocos, 10 parts of pinellia ternata, 5 parts of immature bitter orange, 10 parts of stir-fried spinach seeds, 3 parts of roasted ginger, 5 parts of schisandra chinensis, 5 parts of angelica sinensis, 5 parts of raw atractylodes macrocephala, 3 parts of magnolia bark, 3 parts of roasted liquorice root, 5 parts of poria, 5 parts of cinnamon twig, 5 parts of green peel, 5 parts of bupleurum chinense, 10 parts of moutan bark, 3 parts of gardenia jasmine, 5 parts of mint, 5 parts of white peony root, 10 parts of raw rehmannia root, and 100 parts of honey.

3. A liver-clearing, restlessness-relief and mind-calming paste according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 7.5 parts of dried tangerine peel, 22.5 parts of poria cocos, 11.5 parts of pinellia ternata, 7.5 parts of immature bitter orange, 11.5 parts of stir-fried spinach seeds, 5 parts of roasted ginger, 7.5 parts of schisandra chinensis, 9.5 parts of angelica sinensis, 7 parts of raw atractylodes and magnolia bark, 5 parts of roasted liquorice, 7.5 parts of poria, 7.5 parts of cinnamon twig, 7.5 parts of green peel, 9.5 parts of bupleurum, 11.5 parts of moutan bark, 5 parts of gardenia, 5.5 parts of mint, 9.5 parts of white peony root, 22.5 parts of raw rehmannia root, and 115 parts of honey.

4. A liver-clearing, restlessness-relief and mind-calming paste according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 10 parts of dried tangerine peel, 30 parts of poria, 15 parts of pinellia ternata, 10 parts of immature bitter orange, 15 parts of stir-fried spinach seeds, 6.5 parts of roasted ginger, 10 parts of schisandra chinensis, 12.5 parts of angelica sinensis, 12.5 parts of raw atractylodes macrocephala, 9 parts of magnolia bark, 6.5 parts of roasted licorice root, 10 parts of poria, 10 parts of cinnamon twig, 10 parts of green peel, 12.5 parts of bupleurum, 15 parts of moutan bark, 6.5 parts of gardenia, 7.5 parts of mint, 12.5 parts of white peony root, 30 parts of raw rehmannia, and 150 parts of honey.

5. The liver-clearing, restlessness-relief and mind-calming paste according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 12.5 parts of dried tangerine peel, 37.5 parts of poria, 19 parts of pinellia ternata, 12.5 parts of immature bitter orange, 19 parts of stir-fried spinach seeds, 8 parts of roasted ginger, 12.5 parts of schisandra chinensis, 15.5 parts of angelica sinensis, 15.5 parts of raw atractylodes macrocephala, 11.5 parts of magnolia bark, 8 parts of roasted liquorice root, 12.5 parts of poria, 12.5 parts of cinnamon twig, 12.5 parts of green peel, 15.5 parts of bupleurum, 19 parts of moutan bark, 8 parts of gardenia, 9.5 parts of mint, 15.5 parts of white peony root, 37.5 parts of raw rehmannia root, and 190 parts of honey.

6. A liver-clearing, restlessness-relief and mind-calming paste according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 15 parts of dried tangerine peel, 45 parts of poria, 20 parts of pinellia ternata, 15 parts of immature bitter orange, 20 parts of stir-fried spinach seeds, 10 parts of roasted ginger, 15 parts of schisandra chinensis, 20 parts of raw atractylodes macrocephala, 15 parts of magnolia bark, 10 parts of roasted liquorice root, 15 parts of poria, 15 parts of cinnamon twig, 15 parts of green peel, 20 parts of bupleurum, 20 parts of moutan bark, 10 parts of gardenia, 10 parts of mint, 20 parts of white peony root, 50 parts of raw rehmannia root, and 200 parts of honey.

7. A method for preparing the liver-clearing, vexation-removing and mind-calming paste according to any one of claims 1 to 6, characterized in that: It includes the following steps: 7.1 Weigh tangerine peel, Poria cocos, Pinellia ternata, Citrus aurantium, Rhizoma Zingiberis, Schisandrae Chinensis, Chinese Angelicae Sinensis, Atractylodes macrocephala, Magnolia officinalis, Roasted Licorice, Poria cocos, Cinnamon twig, Citrus aurantium, Bupleurum chinense, Cortex Moutan, Gardenia jasminoides, Menthol, White Peony Root, and Rehmannia glutinosa together in proportion by weight and soak for 7-9 hours; 7.2 Stir-fry the spinach seeds to extract the fat-soluble tranquilizing ingredients. Then, use water to extract the water-soluble ingredients from the filtered medicinal residue for later use. 7.3 After soaking the Chinese medicinal materials in step 7.1, place them in a pot and simmer for two times, each time for 1.3-1.7 hours. Add mint 7-13 minutes before the end of the second decocting. 7.4 Add the water-soluble ingredients obtained in step 7.2 to the medicinal liquid obtained after decoction in step 7.3, and then filter it 2-3 times. Concentrate the filtrate until it becomes a clear paste in the shape of honeycomb balls. Reduce the heat and add the melted honey. Keep stirring and control the temperature at 70-80℃ until the paste is formed.

8. The preparation method of the liver-clearing, vexation-relief and tranquilizing paste according to claim 7, characterized in that: Poria cocos was freeze-dried using vacuum technology before step 7.1 to preserve its active ingredients; In step 7.1, put dried tangerine peel, Poria cocos, Pinellia ternata, Citrus aurantium, Rhizoma Zingiberis, Schisandrae Chinensis, Chinese Angelicae Sinensis, Atractylodes macrocephala, Magnolia officinalis, Roasted Licorice, Poria, Cinnamon twig, Citrus aurantium, Bupleurum chinense, Cortex Moutan, Gardenia jasminoides, Mentha, White Peony Root, and Rehmannia glutinosa into a cloth bag and soak it in purified water 6-8 times the weight of the medicinal materials in this step for 8 hours; In step 7.2, a predetermined amount of stir-fried spinach seeds is weighed and subjected to a component extraction process using a combined alcohol extraction-water extraction process, namely, based on different solvent solubilities, first using an ethanol-water solution with a volume ratio of water to ethanol of 70% by volume at 70-80° C., wherein the weight ratio of the stir-fried spinach seeds to the ethanol-water solution is 1:8-10, reflux for 1.9-2.1 hours, and repeated twice, and then concentrated under reduced pressure at 55° C.-65° C. to remove alcohol to obtain the fat-soluble sedative component of the stir-fried spinach seeds; the spinach seeds residue after the fat-soluble sedative component is extracted is filtered and separated, and washed thoroughly with pure water until no ethanol residue is left, and then the spinach seeds residue is decocted with 6-8 times the weight of the spinach seeds residue at normal pressure of 90±2° C. for 1.4-1.6 hours to obtain the water-soluble component of the residue; In step 7.1, simmer for 1.5 hours twice, and add mint 10 minutes before the end of the second simmering. In step 7.4, filter 2-3 times with a sieve of 80 to 100 mesh. The paste is collected by using the standard of water droplets forming beads and hanging flags. After the paste is collected, it is packaged into sterilized glass bottles while hot and sterilized with high-pressure steam at 115℃-125℃ for 20-30 minutes. After the paste is cooled, it can be sealed.

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