Bactericidal composition containing kasugamycin and oxine-copper and preparation method thereof

Abstract

The invention relates to the field of insecticidal compositions, and particularly discloses a bactericidal composition containing kasugamycin and oxine-copper and a preparation method of the bactericidal composition. The bactericidal composition containing kasugamycin and oxine-copper is prepared from the following raw materials in parts by weight: 4 to 5 parts of kasugamycin, 40 to 42 parts of oxine-copper, 10 to 20 parts of natural extracting solution, 1 to 3 parts of dispersing agent, 1 to 2 parts of thickening agent, 0.2 to 0.5 part of wetting agent and 45 to 50 parts of water, the preparation method comprises the following steps: uniformly mixing and stirring kasugamycin and water, then adding oxine-copper, and uniformly mixing and stirring to obtain a primary mixture; adding a natural extracting solution, a dispersing agent, a thickening agent and a wetting agent into the primary mixture, and continuously mixing and uniformly stirring to obtain the bactericidal composition; the bactericidal composition has the advantages of high efficiency, long lasting period and environment friendliness, and can effectively prevent and treat angular leaf spot, canker and downy mildew.

Description

Technical Field

[0001] The present application relates to the field of insecticide compositions, and more particularly, to a fungicidal composition containing kasugamycin and quinoline copper and a preparation method thereof. Background Art

[0002] Common bacterial diseases in agricultural production, such as angular leaf spot, canker, and downy mildew, pose a serious threat to crop yield and quality. Existing fungicides mostly focus on the control of fungal diseases, while the prevention and control measures for bacterial diseases are limited. Fungicides on the market are often ineffective due to their lack of specificity.

[0003] Although existing bactericidal compositions for bacterial diseases have certain bactericidal effects, they have problems such as short duration of effectiveness and high risk of environmental pollution. At the same time, single-ingredient bactericides can easily cause pathogens to develop resistance. Although compound bactericides can alleviate this situation to a certain extent, they still face the dual pressures of reduced efficacy and increased costs in actual applications.

[0004] Therefore, it is a problem to be solved to develop a bacterial bactericidal composition that is highly effective, long-lasting, and environmentally friendly and can effectively prevent and treat angular leaf spot, canker, and downy mildew. Summary of the Invention

[0005] In order to prepare a bacterial bactericidal composition that is highly effective, long-lasting, and environmentally friendly, and can effectively prevent and treat angular leaf spot, canker, and downy mildew, the present application provides a bactericidal composition containing kasugamycin and quinoline copper and a preparation method thereof.

[0006] In a first aspect, the present application provides a fungicidal composition containing kasugamycin and quinoline copper, which adopts the following technical solution: A fungicidal composition containing kasugamycin and quinoline copper comprises the following raw materials in parts by weight: 4-5 parts of kasugamycin, 40-42 parts of quinoline copper, 10-20 parts of a natural extract, 1-3 parts of a dispersant, 1-2 parts of a thickener, 0.2-0.5 parts of a wetting agent, and 45-50 parts of water.

[0007] By adopting the above technical solution, kasugamycin and quinoline copper are combined, and the systemic absorption and permeability of kasugamycin are utilized to enable plant leaves to quickly absorb and conduct in the plant body, which allows kasugamycin to enter the internal tissues of the plant, interfere with the amino acid metabolism of bacteria, inhibit protein synthesis, cause the hyphae to swell and degenerate, and stop growing, thereby inhibiting and killing the pathogens inside the plant; in combination with quinoline copper, copper ions are gradually released, and after the copper ions enter the bacterial cell through the protoplasm membrane, the bacterial protoplasm and protein are passivated, gradually killing bacteria in the surface tissues of plant leaves, and can also inhibit the metabolism of pathogenic spores, control division and differentiation, and further organize the spread and reproduction of bacteria on the surface of plant leaves; that is, kasugamycin and quinoline copper improve the plant's prevention and control effect on angular leaf spot, ulcer disease, and downy mildew by simultaneously killing and inhibiting bacteria inside and on the surface of plant leaves.

[0008] Kasugamycin, a natural extract, a dispersant, and a wetting agent are combined, and the penetration-promoting effect of the wetting agent is utilized in combination with the penetration effect of kasugamycin and the natural extract, so that kasugamycin and the natural extract gradually penetrate into the interior of plant leaves, thereby improving the bactericidal effect. In addition, the wetting agent can increase the contact area between the bactericidal composition and the surface of the plant leaves, and combined with the dispersant, the bactericidal composition is evenly attached to the surface of the plant leaves, thereby achieving the effect of uniformly preventing and controlling bacterial diseases.

[0009] Quinoline copper and a thickener are combined. After the fungicide composition is evenly dispersed on the surface of plant leaves, as kasugamycin and the natural extract penetrate into the interior of the plant leaves, quinoline copper and the thickener gradually form a protective film on the surface of the plant leaves. This film not only has a high affinity with the plant leaves, allowing the fungicide composition to stably adhere to the surface of the plant leaves, but also can improve the rain erosion resistance of the fungicide composition, achieving the effect of long-term inhibition of the germination and invasion of other bacteria, thereby extending the prevention and control time of angular leaf spot, canker, and downy mildew.

[0010] Preferably, the kasugamycin is composed of kasugamycin liquid and sustained-release kasugamycin in a mass ratio of 1:0.2-0.3.

[0011] By adopting the above technical scheme, kasugamycin liquid and sustained-release kasugamycin are combined. When the bactericidal composition is configured into a suspension and other substances and sprayed on the surface of plant leaves, the water solubility of kasugamycin is first utilized to quickly dissolve in water and adhere to the surface of the plant leaves. Combined with the film-forming effect of the thickener and quinoline copper, kasugamycin and sustained-release kasugamycin are relatively stably adhered to the surface of the plant leaves. In the subsequent long-term prevention and control process, the sustained-release kasugamycin can be slowly released to achieve long-term antibacterial and bactericidal effects, thereby improving the plant's prevention and control effects on angular leaf spot, canker, and downy mildew.

[0012] Preferably, the sustained-release kasugamycin is prepared by soaking and dispersing porous carbon in an isomaltotriose solution, filtering, drying, then soaking and dispersing in a kasugamycin solution, and then binding the surface with a sulfamic acid complex liquid after filtration; the mass ratio of porous carbon, isomaltotriose solution, kasugamycin solution, and sulfamic acid complex liquid is 1:3-5:8-10:1-1.5.

[0013] By adopting the above technical solution, the low viscosity of the isomaltotriose solution is utilized to facilitate the adsorption of the isomaltotriose solution by the porous carbon. After drying, the isomaltotriose adheres to the interior of the porous carbon. Then, the adsorption effect of the porous carbon is utilized to further adsorb the kasugamycin solution. The hydroxyl groups in the isomaltotriose are further attracted and connected with the hydroxyl groups in the kasugamycin, thereby improving the adsorption stability of kasugamycin in the porous carbon and prolonging the sustained-release time of kasugamycin.

[0014] The porous carbon is subjected to surface adhesion treatment using a sulfamic acid composite liquid. The sulfamic acid is acidic, and kasugamycin has good stability under acidic conditions and is easily decomposed under alkaline conditions. The acidic film layer of the sulfamic acid composite liquid is used to block kasugamycin, further ensuring the stability of kasugamycin in the porous carbon. When the suspension prepared from the fungicide composition is sprayed on the surface of plant leaves, kasugamycin can be slowly released in the porous carbon, thereby extending the prevention and control time of plant leaf diseases.

[0015] Preferably, the aminosulfonic acid composite liquid is prepared from aminosulfonic acid, hydroxyethyl cellulose and water in a mass ratio of 1:0.5-1:150-200.

[0016] By adopting the above technical scheme, the solution prepared by dissolving aminosulfonic acid in water is acidic, and hydroxyethyl cellulose has a good bonding and film-forming effect after being dissolved in water. When the aminosulfonic acid composite liquid adheres to the surface of the porous carbon, the blocking effect of the aminosulfonic acid composite liquid after film formation is utilized to ensure the sustained release and stability of kasugamycin, and the amino and sulfonic acid groups in the aminosulfonic acid are combined with the hydroxyl groups in the hydroxyethyl cellulose to increase the cross-linking density of the film layer on the surface of the porous carbon, thereby improving the blocking effect of the film layer on kasugamycin, ensuring that kasugamycin has a longer duration of action, and can prolong the disease prevention and control time of plant leaves.

[0017] Preferably, the quinoline copper is composed of quinoline copper particles and coated slow-release quinoline copper in a mass ratio of 1:0.5-1.

[0018] By adopting the above technical solution, quinoline copper particles and coated slow-release quinoline copper are combined. First, the copper ions released from the quinoline copper particles are used to achieve rapid bactericidal and antibacterial effects on the surface of plant leaves. As the copper ions in the coated slow-release quinoline copper are gradually released and react with carbon dioxide in the air to generate carbonic acid, this process not only enhances the stability of the drug film, but also improves the bactericidal efficiency of quinoline copper, thereby extending the prevention and control time and improving the prevention and control effect.

[0019] Preferably, the coated sustained-release quinoline copper is prepared from quinoline copper, linseed gum solution, and pine ester alcohol diglucoside in a mass ratio of 1:0.8-1.6:0.2-0.4.

[0020] By adopting the above technical solution, the surface of the quinoline copper is bonded with a linseed gum solution and then loaded with pinoresinol diglucoside. The lipophilic effect of pinoresinol diglucoside is utilized to facilitate attachment to the lipophilic surface of plant leaves. The hydrophilic hydroxyl groups of the pinoresinol diglucoside are utilized to facilitate cross-linking with the hydroxyl groups in the linseed gum. The hydroxyl groups and amino groups in the slow-release kasugamycin can also be cross-linked. This further improves the adhesion stability of the insecticide composition on the surface of plant leaves. In combination with the slow-release quinoline copper, the prevention and control time of diseases on the surface of plant leaves is prolonged.

[0021] Flaxseed gum is insoluble in room temperature water. During the preparation of the bactericidal composition and the suspending agent, flaxseed gum is not easily soluble in room temperature water, thereby affecting the sustained-release effect of the coated sustained-release quinoline copper. In addition, the film-forming stability of the pine ester alcohol diglucoside and flaxseed gum solution is utilized to further improve the water scouring resistance of the surface of the bactericidal composition, and the composition can also resist external pathogens, thereby extending the effectiveness of the bactericidal composition in preventing and controlling plant diseases.

[0022] Pinesterol diglucoside combined with natural extract can affect the digestive system of pests, thus having certain anti-insect activity. Combined with the film-forming effect of quinoline copper, it further improves the insect resistance and stability of the fungicidal composition, and still has a good control effect under long-term use conditions.

[0023] Preferably, the flaxseed gum solution is prepared from flaxseed gum, fucoidan and water in a mass ratio of 1:0.2-0.5:150-200.

[0024] By adopting the above technical solution, flaxseed gum and fucoidan are not easily soluble in room temperature water. Under the conditions of preparing the bactericidal composition and the suspension concentrate prepared by the bactericidal composition, room temperature water is unlikely to affect the film formation of the flaxseed gum solution, and the moisture in the environment is also room temperature water, which generally does not reach above 60°C, thereby ensuring the sustained-release blocking effect of the flaxseed gum solution on quinoline copper and prolonging the prevention and control of plant diseases by the bactericidal composition.

[0025] Fucoidan has certain antibacterial activity and can inhibit diseases and kill some bacteria. Combined with the antibacterial effects of quinoline copper and pinoresinol diglucoside, it can further destroy bacterial cell membranes, accelerate bacterial death, improve crop immunity, and promote crop growth.

[0026] Preferably, the natural extract is prepared from turmeric extract, sophora flavescens extract, shellac and ethanol in a mass ratio of 1:0.8-1.5:0.2-0.5:150-250.

[0027] By adopting the above technical solution, turmeric extract, sophora flavescens extract and shellac are all dissolved in ethanol, and shellac is used to increase the viscosity of the natural extract, thereby facilitating the stable attachment of the turmeric extract and the sophora flavescens extract to the surface of plant leaves. The combination of the bacterial control effect of curcumin in turmeric and the bacterial and pest control effect of matrine further improves the bactericidal and antibacterial effect of the fungicide composition. In addition, shellac has a certain viscosity and can stably attach to the surface of plant leaves. Combined with the attachment and film-forming properties of shellac on the surface of plant leaves, bacteria and pests in the external environment are further blocked, thereby protecting the health of plant leaves, improving the control effect and extending the control time.

[0028] The natural extract has a good penetration effect under the action of ethanol. Combined with the penetration effect of kasugamycin, it further improves the antibacterial and bactericidal effect inside the plant, thereby improving the prevention and control effect.

[0029] Preferably, the wetting agent is hydroxyapatite whiskers.

[0030] By adopting the above technical solution, when the fungicide composition is configured into a suspension and sprayed onto the surface of plant leaves, the thorn-like structure of the hydroxyapatite whiskers is utilized to facilitate the generation of micro-scratches on the surface of the plant leaves, further promoting the penetration effect of the copper ions in kasugamycin and quinoline copper into the interior of the plant leaves, thereby improving the bactericidal and bacteriostatic effects; and the micro-scratches of the hydroxyapatite whiskers are utilized to further increase the contact area between the fungicide composition and the surface of the plant leaves, and can also improve the adhesion stability of the fungicide composition on the surface of the plant leaves, thereby further extending the bactericidal and bacteriostatic time of the fungicide composition on the surface of the plant leaves, thereby extending the prevention and control time.

[0031] In a second aspect, the present application provides a method for preparing a fungicidal composition containing kasugamycin and quinoline copper, using the following technical solution: A method for preparing a bactericidal composition containing kasugamycin and quinoline copper comprises the following steps: S1, kasugamycin and water are mixed and stirred evenly, and then copper quinoline is added and mixed and stirred evenly to obtain an initial mixture; S2. Add the natural extract, dispersant, thickener and wetting agent to the primary mixture and continue to mix and stir evenly to obtain a bactericidal composition.

[0032] By adopting the above technical solution, the prepared bactericidal composition has the advantages of high bactericidal efficiency, low toxicity and environmental friendliness.

[0033] In summary, this application has the following beneficial effects: 1. Kasugamycin and quinoline copper cooperate with each other, and the systemic absorption and permeability of kasugamycin are utilized to enable plant leaves to quickly absorb and conduct in the plant body, which allows kasugamycin to enter the internal tissues of the plant, interfere with the amino acid metabolism of bacteria, inhibit protein synthesis, cause the hyphae to swell and degenerate, and stop growth, thereby inhibiting and killing pathogens inside the plant; in combination with quinoline copper, copper ions are gradually released, and after copper ions enter the bacterial cell through the protoplasm membrane, the bacterial protoplasm and protein are passivated, gradually killing bacteria on the surface tissues of plant leaves, and can also inhibit the metabolism of pathogenic spores, control division and differentiation, and further organize the spread and reproduction of bacteria on the surface of plant leaves; that is, kasugamycin and quinoline copper improve the plant's prevention and control effect on angular leaf spot, ulcer disease, and downy mildew by synchronously killing and inhibiting bacteria inside and on the surface of plant leaves.

[0034] 2. Kasugamycin, natural extract, dispersant and wetting agent are combined, and the penetration-promoting effect of the wetting agent is utilized in combination with the penetration effect of kasugamycin and natural extract, so that kasugamycin and natural extract gradually penetrate into the interior of the plant leaves, thereby improving the bactericidal effect; and the wetting agent can increase the contact area between the bactericidal composition and the surface of the plant leaves, and cooperate with the dispersant to make the bactericidal composition evenly adhere to the surface of the plant leaves, thereby achieving the effect of uniformly preventing and controlling bacterial diseases. DETAILED DESCRIPTION

[0035] The present application is further described in detail below with reference to the embodiments.

[0036] Preparation Example of Sustained-Release Kasugamycin Among the following raw materials, kasugamycin was purchased from Suzhou Chengjie Fine Chemical Co., Ltd.; other raw materials were commercially available.

[0037] Preparation Example 1: Sustained-release kasugamycin was prepared by the following method: 1 kg of aminosulfonic acid and 0.8 kg of hydroxyethyl cellulose were placed in 180 kg of water and stirred until completely dissolved to obtain an aminosulfonic acid composite liquid; 1 kg of porous carbon is immersed in 4 kg of isomaltotriose solution, the porous carbon is passed through a 150-mesh sieve, the average porosity is 75%, isomaltotriose is a 1% by mass isomaltotriose aqueous solution, ultrasonically dispersed at 20 kHz for 5 minutes, and then the porous carbon is filtered out, and after drying, the loaded porous carbon is obtained; the loaded porous carbon is immersed in 9 kg of kasugamycin solution, the kasugamycin solution is a 12% by mass kasugamycin aqueous solution, ultrasonically dispersed at 20 kHz for 10 minutes, and then the loaded porous carbon is filtered out to obtain a semi-finished product; 1.2 kg of aminosulfonic acid complex liquid is evenly sprayed on the surface of the semi-finished product, and after drying and dispersion, sustained-release kasugamycin is obtained; the sustained-release kasugamycin is passed through a 75-mesh sieve.

[0038] Preparation Example 2: This preparation example differs from Preparation Example 1 in that: 1 kg of aminosulfonic acid and 0.5 kg of hydroxyethyl cellulose were placed in 150 kg of water and stirred until completely dissolved to obtain an aminosulfonic acid composite liquid; 1 kg of porous carbon was immersed in 3 kg of isomaltotriose solution, ultrasonically dispersed at 20 kHz for 5 minutes, and then the porous carbon was filtered out. After heating and drying, the loaded porous carbon was obtained. The loaded porous carbon was immersed in 8 kg of kasugamycin solution, ultrasonically dispersed at 20 kHz for 10 minutes, and then the loaded porous carbon was filtered out to obtain a semi-finished product. 1 kg of aminosulfonic acid complex liquid was evenly sprayed on the surface of the semi-finished product, and after drying and dispersion, sustained-release kasugamycin was obtained.

[0039] Preparation Example 3: This preparation example differs from Preparation Example 1 in that: 1 kg of aminosulfonic acid and 1 kg of hydroxyethyl cellulose were placed in 200 kg of water and stirred until completely dissolved to obtain an aminosulfonic acid composite liquid; 1 kg of porous carbon was immersed in 5 kg of isomaltotriose solution, ultrasonically dispersed at 20 kHz for 5 minutes, and then the porous carbon was filtered out. After heating and drying, the loaded porous carbon was obtained. The loaded porous carbon was immersed in 10 kg of kasugamycin solution, ultrasonically dispersed at 20 kHz for 10 minutes, and then the loaded porous carbon was filtered out to obtain a semi-finished product. 1.5 kg of aminosulfonic acid complex liquid was evenly sprayed on the surface of the semi-finished product, and after drying and dispersion, sustained-release kasugamycin was obtained.

[0040] Preparation example of coated sustained-release quinoline copper Among the following raw materials, flaxseed gum was purchased from Shandong Xinxiong Biotechnology Co., Ltd.; fucoidan was purchased from Hebei Qiansheng Biotechnology Co., Ltd.; quinoline copper was purchased from 8-hydroxyquinoline copper of Wuhan Smack Biotechnology Co., Ltd.; other raw materials were all commercially available.

[0041] Preparation Example 4: Coated sustained-release quinoline copper was prepared by the following method: Mix 1 kg of flaxseed gum and 0.35 kg of fucoidan, add 180 kg of water at 85°C, and mix and stir until the flaxseed gum and fucoidan are completely dissolved to obtain a flaxseed gum solution; 1.2 kg of linseed gum solution was evenly sprayed on the surface of 1 kg of quinoline copper, the quinoline copper was passed through a 200-mesh sieve, and then 0.3 kg of pinoresinol diglucoside was added, and the pinoresinol diglucoside was passed through a 300-mesh sieve. The addition rate of pinoresinol diglucoside was 100 g / min. During the addition process, the quinoline copper was continuously stirred at a speed of 120 r / min. After uniform mixing, the solution was dried and dispersed to obtain a coated slow-release quinoline copper; the coated slow-release quinoline copper was passed through a 100-mesh sieve.

[0042] Preparation Example 5: This preparation example differs from Preparation Example 4 in that: Mix 1 kg of flaxseed gum and 0.2 kg of fucoidan, add 150 kg of water at 85°C, and stir until the flaxseed gum and fucoidan are completely dissolved to obtain a flaxseed gum solution; 0.8 kg of linseed gum solution was evenly sprayed on the surface of 1 kg of quinoline copper, and then 0.2 kg of pinoresinol diglucoside was added at a rate of 100 g / min. During the addition process, the quinoline copper was continuously stirred at a speed of 120 r / min. After uniform mixing, it was dried and dispersed to obtain coated sustained-release quinoline copper.

[0043] Preparation Example 6: This preparation example differs from Preparation Example 4 in that: After mixing 1 kg of flaxseed gum and 0.5 kg of fucoidan, add 200 kg of water at 85°C, mix and stir until the flaxseed gum and fucoidan are completely dissolved to obtain a flaxseed gum solution; 1.6 kg of linseed gum solution was evenly sprayed on the surface of 1 kg of quinoline copper, and then 0.4 kg of pinoresinol diglucoside was added at a rate of 100 g / min. During the addition process, the quinoline copper was continuously stirred at a speed of 120 r / min. After uniform mixing, it was dried and dispersed to obtain coated sustained-release quinoline copper.

[0044] Preparation example of natural extract Among the following raw materials, turmeric extract was purchased from Hebei Jiuyu Biotechnology Co., Ltd.; Sophora flavescens extract was purchased from Shaanxi Kepler Biotechnology Co., Ltd.; other raw materials were commonly available on the market.

[0045] Preparation Example 7: The natural extract was prepared by the following method: 1 kg of turmeric extract, 1.2 kg of Sophora flavescens extract and 0.3 kg of shellac were mixed and stirred evenly, and then 200 kg of ethanol was added and stirred until the turmeric extract, Sophora flavescens extract and shellac were completely dissolved and the mass fraction of ethanol was 99%, thereby obtaining a natural extract.

[0046] Preparation Example 8: This preparation example differs from Preparation Example 7 in that: 1 kg of turmeric extract, 0.8 kg of Sophora flavescens extract and 0.2 kg of shellac were mixed and stirred evenly, and then 150 kg of ethanol was added and stirred until the turmeric extract, Sophora flavescens extract and shellac were completely dissolved to obtain a natural extract.

[0047] Preparation Example 9: This preparation example differs from Preparation Example 7 in that: 1 kg of turmeric extract, 1.5 kg of Sophora flavescens extract and 0.5 kg of shellac were mixed and stirred evenly, and then 250 kg of ethanol was added and stirred until the turmeric extract, Sophora flavescens extract and shellac were completely dissolved to obtain a natural extract. Example

[0048] The following raw materials are all commercially available.

[0049] Example 1: A bactericidal composition containing kasugamycin and quinoline copper: 4.5 kg of kasugamycin, 41 kg of quinoline copper, 15 kg of natural extract, 2 kg of dispersant, 1.6 kg of thickener, 0.4 kg of wetting agent, and 48 kg of water; kasugamycin is composed of kasugamycin solution and sustained-release kasugamycin prepared in Preparation Example 1 at a mass ratio of 1:0.25; quinoline copper is composed of quinoline copper particles and coated sustained-release quinoline copper prepared in Preparation Example 4 at a mass ratio of 1:0.8; the natural extract is the natural extract prepared in Preparation Example 7; the dispersant is sodium dodecylbenzenesulfonate; the thickener is a 1% by mass carboxymethyl cellulose solution; the wetting agent is hydroxyapatite whiskers, the average length of the hydroxyapatite whiskers is 40 μm, and the average diameter is 10 nm; The preparation method is as follows: S1, kasugamycin and water are mixed and stirred evenly, and then copper quinoline is added and mixed and stirred evenly to obtain an initial mixture; S2. Add the natural extract, dispersant, thickener and wetting agent to the primary mixture and continue to mix and stir evenly to obtain a bactericidal composition.

[0050] Example 2: This example differs from Example 1 in that: 4 kg of kasugamycin, 40 kg of quinoline copper, 10 kg of natural extract, 1 kg of dispersant, 1 kg of thickener, 0.2 kg of wetting agent, and 45 kg of water; kasugamycin is composed of kasugamycin liquid and the sustained-release kasugamycin prepared in Preparation Example 2 in a mass ratio of 1:0.2; quinoline copper is composed of quinoline copper particles and the coated sustained-release quinoline copper prepared in Preparation Example 5 in a mass ratio of 1:0.5; the natural extract is the natural extract prepared in Preparation Example 8.

[0051] Example 3: This example differs from Example 1 in that: 5kg of kasugamycin, 42kg of quinoline copper, 20kg of natural extract, 3kg of dispersant, 2kg of thickener, 0.5kg of wetting agent, and 50kg of water; kasugamycin is composed of kasugamycin liquid and sustained-release kasugamycin prepared in Preparation Example 3 at a mass ratio of 1:0.3; quinoline copper is composed of quinoline copper particles and coated sustained-release quinoline copper prepared in Preparation Example 6 at a mass ratio of 1:1; the natural extract is the natural extract prepared in Preparation Example 9.

[0052] Example 4: This example differs from Example 1 in that: Sustained-release kasugamycin is not added to kasugamycin.

[0053] Example 5: This example differs from Example 1 in that: No isomaltotriose solution and aminosulfonic acid complex solution were added during the preparation of sustained-release kasugamycin.

[0054] Example 6: This example differs from Example 1 in that: No hydroxyethyl cellulose was added to the aminosulfonic acid complex solution during the preparation of sustained-release kasugamycin.

[0055] Example 7: This example differs from Example 1 in that: Coated sustained-release quinoline copper is not added to quinoline copper.

[0056] Example 8: This example differs from Example 1 in that: No rosinol diglucose was added during the preparation of coated sustained-release quinoline copper.

[0057] Example 9: This example differs from Example 1 in that: No fucoidan was added during the preparation of flaxseed gum solution in coated sustained-release quinoline copper.

[0058] Example 10: This example differs from Example 1 in that: Natural extracts with no added shellac.

[0059] Comparative Example Comparative Example 1: The difference between this comparative example and Example 1 is that: No wetting agents were added to the raw materials.

[0060] Comparative Example 2: This comparative example differs from Example 1 in that: No kasugamycin was added to the raw materials.

[0061] Comparative Example 3: This comparative example differs from Example 1 in that: No quinoline copper was added to the raw materials.

[0062] Performance testing 1. Prevention and control effect detection The methods of Examples 1-10 and Comparative Examples 1-3 were used to prepare fungicidal compositions, respectively. Water was added to the fungicidal compositions to prepare suspensions, and the fungicidal compositions were diluted 500 times. One fungicidal composition was used for each acre of land, and the dosage of each group of liquid was 45 liters per acre, and the suspensions were evenly sprayed. Plant angular leaf spot control effect: The plants are cucumber seedlings, and the surface of the cucumber seedlings is inoculated with a downy mildew suspension (bacterial activity 1000 CFU / mL). 25 μL of the downy mildew suspension is inoculated on cucumber leaves with a diameter of 20 mm. The first application is made at the early stage of the disease, and the second application is made after an interval of 7 days. The disease index is investigated before application, and the control effect is investigated 14 days and 40 days after the last application. Ten points are randomly selected per mu, and 10 plants are investigated at each point. The disease index is graded according to the percentage of the lesion area on each leaf to the total leaf area. The blank unsprayed group is used as the control group. The disease grading standard is as follows: Grade 0: no lesions; Grade 1: the lesion area accounts for less than 5% of the total leaf area; Grade 3: the lesion area accounts for 6% to 10% of the total leaf area; Grade 5: the lesion area accounts for 11% to 20% of the total leaf area; Grade 7: the lesion area accounts for 21% to 50% of the total leaf area; Grade 9: the lesion area accounts for more than 51% of the total leaf area.

[0063] Disease index = ∑ (number of diseased leaves at each level × relative level value) / total number of leaves surveyed × highest disease level × 100% Control effect = (control disease index - drug treatment disease index) / control disease index × 100%.

[0064] Citrus canker control efficacy: Citrus seedlings were used as plants, and a canker suspension (1000 CFU / mL) was inoculated on the surface of the seedlings. Citrus leaves with a diameter of 20 mm were inoculated with 20 μL of downy mildew suspension. The first application was made at the early stage of the disease, and the second application was made 7 days later. The disease index was investigated before application, and the control efficacy was investigated 14 and 40 days after the last application. Ten points were randomly selected per mu, and 10 plants were investigated at each point. The canker area on each leaf was classified as a percentage of the total leaf area. A blank, unsprayed group served as the control group. The disease grading standards are as follows: Level 0: no ulcers; Level 1: the ulcer area accounts for less than 5% of the total leaf area; Level 3: the ulcer area accounts for 6% to 10% of the total leaf area; Level 5: the ulcer area accounts for 11% to 20% of the total leaf area; Level 7: the ulcer area accounts for 21% to 50% of the total leaf area; Level 9: the ulcer area accounts for more than 51% of the total leaf area.

[0065] Disease index = ∑ (number of diseased leaves at each level × relative level value) / total number of leaves surveyed × highest disease level × 100% Control effect = (control disease index - drug treatment disease index) / control disease index × 100%.

[0066] Downy mildew control efficacy: Lettuce seedlings were used as plants, and a downy mildew suspension (1000 CFU / mL) was inoculated on the surface of the lettuce. 15 μL of the downy mildew suspension was inoculated onto lettuce leaves 20 mm in diameter. The first application was at the early stage of the disease, and the second application was made 7 days later. The disease index was investigated before application, and the control efficacy was investigated 14 and 40 days after the last application. Ten points were randomly selected per mu, and 10 plants were investigated at each point. The plants were graded based on the percentage of the lesion area per leaf to the total leaf area. A blank, unsprayed group served as the control group. The disease grading standards are as follows: Level 0: no lesions; Level 1: the area of ​​lesions accounts for less than 5% of the total leaf area; Level 3: the area of ​​lesions accounts for 6% to 10% of the total leaf area; Level 5: the area of ​​lesions accounts for 11% to 20% of the total leaf area; Level 7: the area of ​​lesions accounts for 21% to 50% of the total leaf area; Level 9: the area of ​​lesions accounts for more than 51% of the total leaf area.

[0067] Disease index = ∑ (number of diseased leaves at each level × relative disease level) / total number of leaves surveyed × highest disease level × 100% Control effect = (control disease index - drug treatment disease index) / control disease index × 100%.

[0068] Table 1 Performance test table In combination with Examples 1-3 and Table 1, it can be seen that the fungicidal composition prepared in the present application has a good control effect on angular leaf spot, ulcer disease, and downy mildew. Under the control conditions of 14d and 40d, it still has a high control effect, protects plant growth, and increases crop yield.

[0069] Combining Example 1 and Examples 4-10 and Table 1, it can be seen that no sustained-release kasugamycin is added to kasugamycin in Example 4. Compared with Example 1, the prevention effect of Example 4 is lower than that of Example 1; this shows that the addition of sustained-release kasugamycin can prolong the duration of action of the fungicidal composition, and has good prevention and control effects on plant angular leaf spot, canker and downy mildew after 14 days and 40 days, thereby protecting plant growth.

[0070] In the preparation process of the sustained-release kasugamycin in Example 5, no isomaltotriose solution and aminosulfonic acid complex solution were added. Compared with Example 1, the prevention effect of Example 5 was lower than that of Example 1; this indicates that the acidity of aminosulfonic acid stabilizes kasugamycin, and the binding effect of isomaltotriose on kasugamycin further prolongs the effect of kasugamycin on the surface of plant leaves, thereby achieving the advantage of long-term disease prevention and control.

[0071] During the preparation of the sustained-release kasugamycin in Example 6, hydroxyethyl cellulose was not added to the aminosulfonic acid complex liquid. Compared with Example 1, the prevention effect of Example 6 was lower than that of Example 1. This shows that the presence of hydroxyethyl cellulose facilitates the formation of a film layer on the surface of the porous carbon adsorbed with kasugamycin, further sustained-releases kasugamycin, and prolongs the duration of action of kasugamycin, and still has a high prevention and control effect after 40 days.

[0072] In Example 7, no coated slow-release quinoline copper was added. Compared with Example 1, the prevention effect of Example 7 was lower than that of Example 1. This shows that the addition of coated slow-release quinoline copper can further improve the sustained-release effect of quinoline copper, and the continuous antibacterial and bactericidal effects of copper ions enable the fungicidal composition to have a continuous antibacterial and bactericidal effect on the surface of plant leaves. The fungicidal composition has a long-term prevention and control effect on angular leaf spot, ulcer disease, and downy mildew.

[0073] In the preparation process of the coated slow-release quinoline copper in Example 8, no pinoresinol diglucoside was added. Compared with Example 1, the control effect of Example 8 was lower than that of Example 1. This shows that the lipophilic effect of pinoresinol diglucoside is utilized to facilitate attachment to the lipophilic surface of plant leaves, and its hydrophilic hydroxyl group is utilized to facilitate cross-linking with the hydroxyl group in linseed gum, and can also be cross-linked with the hydroxyl group and amino group in the slow-release kasugamycin, thereby further improving the adhesion stability of the insecticide composition on the surface of plant leaves, and combining with the slow-release quinoline copper to prolong the control time of diseases on the surface of plant leaves.

[0074] In the preparation process of the flaxseed gum solution in the coated sustained-release quinoline copper in Example 9, no fucoidan was added. Compared with Example 1, the prevention effect of Example 9 was lower than that of Example 1, which shows that fucoidan has certain antibacterial activity and can inhibit disease and kill some bacteria. Combined with the antibacterial effects of quinoline copper and pinoresinol diglucoside, it further destroys the bacterial cell membrane, accelerates bacterial death, improves crop immunity, and promotes crop growth.

[0075] In Example 10, no shellac was added to the natural extract. Compared with Example 1, the control effect of Example 10 was lower than that of Example 1. This shows that shellac has good adhesion stability, which can facilitate the stable attachment of the turmeric extract and the sophora flavescens extract to the surface of the plant leaves. The combination of the control effect of curcumin in turmeric on bacteria and the control effect of matrine on bacteria and pests further improves the bactericidal and antibacterial effect of the bactericidal composition, and can further block bacteria and pests in the external environment, thereby protecting the health of the plant leaves and improving the control effect.

[0076] Combining Example 1 and Comparative Examples 1-3 and Table 1, it can be seen that no wetting agent is added to the raw materials of Comparative Example 1, and compared with Example 1, the prevention effect of Comparative Example 1 is lower than that of Example 1; this shows that the thorny structure of hydroxyapatite whiskers is used to facilitate the generation of micro-scratches on the surface of plant leaves, further promoting the penetration of copper ions in kasugamycin and quinoline copper into the interior of plant leaves, thereby improving the bactericidal and bacteriostatic effects; and improving the adhesion stability of the bactericidal composition on the surface of plant leaves, thereby further prolonging the bactericidal and bacteriostatic time of the bactericidal composition on the surface of plant leaves, thereby prolonging the prevention and control time.

[0077] No kasugamycin was added to the raw materials of Comparative Example 2. Compared with Example 1, the control effect of Comparative Example 2 was lower than that of Example 1, which showed that kasugamycin had good antibacterial and bactericidal effects and could improve the control effect of plants against angular leaf spot, canker and downy mildew.

[0078] No quinoline copper was added to the raw materials of Comparative Example 3. Compared with Example 1, the control effect of Comparative Example 3 was lower than that of Example 1, indicating that the copper ions of quinoline copper can further inhibit and kill bacteria on the surface of plant leaves, thereby improving the control effect of the fungicidal composition on plant angular leaf spot, ulcer disease, and downy mildew.

[0079] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A bactericidal composition containing kasugamycin and quinoline copper, characterized in that: The invention comprises the following raw materials in parts by weight: 4-5 parts of kasugamycin, 40-42 parts of quinoline copper, 10-20 parts of natural extract, 1-3 parts of dispersant, 1-2 parts of thickener, 0.2-0.5 parts of wetting agent and 45-50 parts of water.

2. A bactericidal composition containing kasugamycin and quinoline copper according to claim 1, characterized in that: The kasugamycin is composed of kasugamycin liquid and sustained-release kasugamycin in a mass ratio of 1:0.2-0.

3.

3. A bactericidal composition containing kasugamycin and quinoline copper according to claim 2, characterized in that: The sustained-release kasugamycin is prepared by soaking and dispersing porous carbon in an isomaltotriose solution, filtering, drying, then soaking and dispersing in the kasugamycin solution, and then binding the surface with a sulfamic acid complex liquid after filtering; the mass ratio of the porous carbon, the isomaltotriose solution, the kasugamycin solution, and the sulfamic acid complex liquid is 1:3-5:8-10:1-1.

5.

4. A bactericidal composition containing kasugamycin and quinoline copper according to claim 3, characterized in that: The aminosulfonic acid composite liquid is prepared from aminosulfonic acid, hydroxyethyl cellulose and water in a mass ratio of 1:0.5-1:150-200.

5. The bactericidal composition containing kasugamycin and quinoline copper according to claim 1, characterized in that: The quinoline copper consists of quinoline copper particles and coated slow-release quinoline copper in a mass ratio of 1:0.5-1.

6. A bactericidal composition containing kasugamycin and quinoline copper according to claim 5, characterized in that: The coated sustained-release quinoline copper is prepared from quinoline copper, linseed gum solution and pine ester alcohol diglucoside in a mass ratio of 1:0.8-1.6:0.2-0.

4.

7. A bactericidal composition containing kasugamycin and quinoline copper according to claim 6, characterized in that: The flaxseed gum solution is prepared from flaxseed gum, fucoidan and water in a mass ratio of 1:0.2-0.5:150-200.

8. The bactericidal composition containing kasugamycin and quinoline copper according to claim 1, characterized in that: The natural extract is prepared from turmeric extract, sophora flavescens extract, shellac and ethanol in a mass ratio of 1:0.8-1.5:0.2-0.5:150-250.

9. The bactericidal composition containing kasugamycin and quinoline copper according to claim 1, characterized in that: The wetting agent is hydroxyapatite whiskers.

10. A method for preparing a bactericidal composition containing kasugamycin and quinoline copper according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1, kasugamycin and water are mixed and stirred evenly, and then copper quinoline is added and mixed and stirred evenly to obtain an initial mixture; S2. Add the natural extract, dispersant, thickener and wetting agent to the primary mixture and continue to mix and stir evenly to obtain a bactericidal composition.

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