Antiviral and bacteriostatic composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of bacteriostatic preparations, and particularly relates to an antiviral and bacteriostatic composition as well as a preparation method and application thereof. The antiviral and bacteriostatic composition is prepared from aprotinin, benzalkonium chloride, zinc gluconate, a plant extract, a flavoring agent and a solvent. The antibacterial rates of the antiviral and antibacterial composition to escherichia coli, staphylococcus aureus, candida albicans and pseudomonas aeruginosa are all higher than 99%, and the antiviral and antibacterial composition has a relatively strong antibacterial effect; the antiviral and bacteriostatic composition disclosed by the invention has a relatively strong inhibition effect on H3N2 and H1N1 influenza viruses; meanwhile, the safety is high, and if the eye drops accidentally enter the eyes, the eye drops have no irritation to the eyes and do not generate side effects of damaging the eyes. According to the antiviral and bacteriostatic composition, the peppermint essence and the aspartame in a specific ratio are combined as the flavoring agent, so that the antiviral and bacteriostatic composition is free of bitter and astringent feeling when being sprayed into the oral cavity and after being sprayed into the oral cavity, and also has proper sweetness.

Description

Technical Field

[0001] The present invention belongs to the technical field of preparations, and in particular relates to an antiviral and antibacterial composition, a preparation method and application thereof. Background Art

[0002] Upper respiratory tract infections (URTIs) are among the most common illnesses, 90% of which are caused by viruses, with bacterial infections often secondary to these. Pathogens primarily invade the nose, pharynx, tonsils, and larynx, causing inflammation, clinically referred to as upper respiratory infections. These infections are common in people of all ages and at all times of the year, when the body's immune system is weakened. Common symptoms of URTIs include congestion and edema of the nasal and pharyngeal mucosa, epithelial cell destruction, small mononuclear cell infiltration, and serous and mucous exudates. Secondary bacterial infections also result in neutrophil infiltration and copious thick secretions, which can easily lead to coughing. Due to the wide variety of viruses that can cause URTIs, diagnosis is challenging, and effective treatment options for viral-induced URTIs remain limited. Currently, antibiotics are often used to treat URTIs, but these treatments are ineffective, and frequent use can lead to increased bacterial resistance. Therefore, the development of highly effective antibacterial and antiviral compositions is crucial for the prevention or treatment of URTIs. Summary of the Invention

[0003] In view of the above problems existing in the prior art, the present invention provides an antiviral and antibacterial composition, a preparation method and application thereof for achieving antibacterial and antiviral effects on the upper respiratory tract.

[0004] The technical solution provided by the present invention is as follows:

[0005] An antiviral and antibacterial composition, characterized by comprising the following components: aprotinin, benzalkonium chloride, and zinc gluconate; a plant extract, a flavoring agent, and a solvent.

[0006] Optionally, a solvent is further included. The antiviral and antibacterial composition comprises the following components per unit volume of solvent: aprotinin 800 KIU / mL~1500 KIU / mL, benzalkonium chloride 800 μg / mL~1200 μg / mL, zinc gluconate 3200 μg / mL~3800 μg / mL, plant extract 250 μg / mL-650 μg / mL, and flavoring agent 2300-9000 μg / mL.

[0007] Optionally, the aprotinin activity is not less than 5400 KIU / mg.

[0008] Optionally, a flavoring agent is further included, and the flavoring agent includes mint essence and aspartame; the antiviral and antibacterial composition contains 2000-5000 μg / mL of mint essence and 300-4000 μg / mL of aspartame, calculated per unit volume of solvent.

[0009] Optionally, plant extracts are further included, including chrysanthemum extract and dandelion extract; the antiviral and antibacterial composition contains 200 μg / mL-500 μg / mL of chrysanthemum extract and 50 μg / mL-150 μg / mL of dandelion extract, calculated per unit volume of solvent.

[0010] Optionally, 5 μg / mL to 15 μg / mL of sodium azulenesulfonate is further included, based on unit volume of the solvent.

[0011] Optionally, an isotonicity agent is also included.

[0012] Optionally, the isotonic agent comprises sodium chloride, and the solvent comprises water.

[0013] Optionally, the solvent comprises, per unit volume, 800 μg / mL to 1200 μg / mL of benzalkonium chloride, 3200 μg / mL to 3800 μg / mL of zinc gluconate, 200 μg / mL to 500 μg / mL of chrysanthemum extract, 50 μg / mL to 150 μg / mL of dandelion extract, 800 KIU / mL to 1500 KIU / mL of aprotinin, 5 μg / mL to 15 μg / mL of sodium azulenesulfonate, 400-900 μg / mL of aspartame, 2500-3500 μg / mL of mint flavor, and 1500-2500 μg / mL of sodium chloride; and the solvent comprises water.

[0014] Optionally, the composition comprises benzalkonium chloride 1000 μg / mL, zinc gluconate 3500 μg / mL, chrysanthemum extract 300 μg / mL, dandelion extract 100 μg / mL, aprotinin 1250 KIU / mL, sodium azulenesulfonate 10 μg / mL, aspartame 600 μg / mL, mint flavor 3000 μg / mL, and sodium chloride 2000 μg / mL, calculated per unit volume of solvent; the solvent comprises water.

[0015] The preparation method of the above-mentioned antiviral and antibacterial composition comprises the following steps:

[0016] Take 70%-90% of the total purified water, add the remaining components in sequence, stir and dissolve; then add the remaining purified water, mix and filter, and the filtrate is the antiviral and antibacterial composition.

[0017] Alternatively, take 70%-90% of the total amount of purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0018] (2) Add mint flavor, aspartame, sodium azulenesulfonate, and zinc gluconate in sequence and stir to dissolve;

[0019] (3) Finally, add chrysanthemum extract and dandelion extract; after dissolving, add the remaining purified water, stir evenly, filter with a membrane, and fill.

[0020] Optionally, the filtration uses a filter membrane with a pore size of 0.8-1 micron, and the filter membrane is a mixed cellulose ester microporous filter membrane.

[0021] Applications of the above antiviral and antibacterial composition: can be used for antibacterial and antiviral inhibition.

[0022] The beneficial effects include at least the following:

[0023] (1) The antiviral and antibacterial composition of the present invention has an antibacterial rate of over 99% against Escherichia coli, Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa, demonstrating a strong antibacterial effect. Furthermore, the antiviral and antibacterial composition of the present invention has a significant inactivation effect on H3N2 and H1N1 influenza viruses, with an inactivation rate of up to 99.99%. When sprayed into the mouth, it has excellent antibacterial and antiviral effects on the upper respiratory tract, effectively improving upper respiratory tract symptoms caused by viruses or bacteria, such as oral ulcers, pharyngitis, sore throat, and tonsillitis.

[0024] (2) The antiviral and antibacterial composition of the present invention is highly safe. When applied as an oral spray, it is non-irritating to the eyes and will not cause any side effects such as eye damage if it accidentally enters the eyes.

[0025] (3) The antiviral and antibacterial composition of the present invention uses a mint flavor and aspartame in a specific ratio as a flavoring agent, which can avoid bitterness when spraying into the mouth and after spraying into the mouth, and also has a suitable sweetness.

[0026] (4) The antiviral and antibacterial composition of the present invention contains a variety of plant ingredients, which have the effects of clearing away heat and detoxifying, soothing and relieving pain DETAILED DESCRIPTION

[0027] The present invention is described in further detail below with reference to specific embodiments:

[0028] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are conventional methods.

[0029] The reagents and materials used in this example can be purchased conventionally. The quantitative experiments involved in the examples were repeated at least three times, and the results were averaged.

[0030] Source of raw materials:

[0031] Chrysanthemum extract and dandelion extract were both food grade and purchased from Shaanxi Bolin Biotechnology Co., Ltd.;

[0032] Mint flavor, food grade, purchased from Hangzhou Bolin Flavor Co., Ltd.;

[0033] Aprotinin; calculated on anhydrous basis, the activity per 1 mg shall not be less than 3.0 units.

[0034] Aprotinin potency assay: One aprotinin activity unit (EPU) is defined as the amount of activity that can inhibit one nucrokatal trypsin (one nucrokatal trypsin unit is defined as the amount of activity that can hydrolyze lumol of N-benzoyl-L-arginine ethyl ester (BAEE) per second). One EPU of aprotinin is equivalent to 1800 KIU.

[0035] MCE filter membrane is a mixed cellulose ester microporous filter membrane with a pore size of 0.8 microns.

[0036] Example 1

[0037] An antiviral and antibacterial composition, comprising:

[0038] Components content Benzalkonium chloride 16mg Aprotinin 30000KIU Sodium azulenesulfonate 0.1mg Sodium chloride 30mg Aspartame 8mg Zinc gluconate 64mg Chrysanthemum Extract 10mg Dandelion extract 1mg mint flavor 70mg purified water 20g

[0039] Preparation method:

[0040] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0041] (2) Add liquid mint flavor, aspartame, sodium azulenesulfonate, and zinc gluconate in sequence and stir to dissolve;

[0042] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolution; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 μm, and the filtrate is the antiviral and antibacterial composition;

[0043] (4) Filling into oral liquid medicinal high-density polyethylene bottle + spray pump can obtain antiviral and antibacterial spray.

[0044] Example 2

[0045] An antiviral and antibacterial composition, comprising:

[0046] Components content Benzalkonium chloride 24mg Aprotinin 16000KIU Sodium azulenesulfonate 0.3mg Sodium chloride 50mg Aspartame 18mg Zinc gluconate 76mg Chrysanthemum Extract 4mg Dandelion extract 3mg mint flavor 50mg purified water 20g

[0047] Preparation method:

[0048] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0049] (2) Add liquid mint flavor, aspartame, sodium azulenesulfonate, and zinc gluconate in sequence and stir to dissolve;

[0050] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolving; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 μm, and the filtrate is the antiviral and antibacterial composition;

[0051] (4) Fill into oral liquid medicinal high-density polyethylene bottle + spray pump to obtain antiviral and antibacterial spray.

[0052] Example 3

[0053] An antiviral and antibacterial composition, comprising:

[0054] Components content Benzalkonium chloride 20mg Aprotinin 25000KIU Sodium azulenesulfonate 0.2mg Sodium chloride 40mg Aspartame 12mg Zinc gluconate 70mg Chrysanthemum Extract 6mg Dandelion extract 2mg mint flavor 60mg purified water 20g

[0055] Preparation method:

[0056] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0057] (2) Add liquid mint flavor, aspartame, sodium azulenesulfonate, and zinc gluconate in sequence and stir to dissolve;

[0058] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolving; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 microns, and the filtrate is the antiviral and antibacterial composition;

[0059] (4) Fill into oral liquid medicinal high-density polyethylene bottle + spray pump to obtain antiviral and antibacterial spray.

[0060] Example 4 (Compared with Example 3, no aspartame was added)

[0061] An antiviral and antibacterial composition, comprising:

[0062] Components content Benzalkonium chloride 20mg Aprotinin 25000KIU Sodium azulenesulfonate 0.2mg Sodium chloride 40mg Zinc gluconate 70mg Chrysanthemum Extract 6mg Dandelion extract 2mg mint flavor 60mg purified water 20g

[0063] Preparation method:

[0064] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0065] (2) Add liquid mint essence, sodium azulene sulfonate, and zinc gluconate in sequence and stir to dissolve;

[0066] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolving; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 μm, and the filtrate is the antiviral and antibacterial composition;

[0067] (4) Fill into oral liquid medicinal high-density polyethylene bottle + spray pump to obtain antiviral and antibacterial spray.

[0068] Example 5 (Compared with Example 3, aspartame was replaced with mint flavor)

[0069] An antiviral and antibacterial composition, comprising:

[0070] Components content Benzalkonium chloride 20mg Aprotinin 25000KIU Sodium azulenesulfonate 0.2mg Sodium chloride 40mg Zinc gluconate 70mg Chrysanthemum Extract 6mg Dandelion extract 2mg mint flavor 72mg purified water 20g

[0071] Preparation method:

[0072] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0073] (2) Add liquid mint essence, sodium azulene sulfonate, and zinc gluconate in sequence and stir to dissolve;

[0074] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolving; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 μm, and the filtrate is the antiviral and antibacterial composition;

[0075] (4) Fill into oral liquid medicinal high-density polyethylene bottle + spray pump to obtain antiviral and antibacterial spray.

[0076] Example 6 (Compared with Example 3, mint flavor was not added)

[0077] An antiviral and antibacterial composition, comprising:

[0078] Components content Benzalkonium chloride 20mg Aprotinin 25000KIU Sodium azulenesulfonate 0.2mg Sodium chloride 40mg Aspartame 12mg Zinc gluconate 70mg Chrysanthemum Extract 6mg Dandelion extract 2mg purified water 20g

[0079] Preparation method:

[0080] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0081] (2) Add liquid mint flavor, aspartame, sodium azulenesulfonate, and zinc gluconate in sequence and stir to dissolve;

[0082] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolving; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 μm, and the filtrate is the antiviral and antibacterial composition;

[0083] (4) Fill into oral liquid medicinal high-density polyethylene bottle + spray pump to obtain antiviral and antibacterial spray.

[0084] Example 7 (Compared with Example 3, mint flavor was replaced with aspartame)

[0085] An antiviral and antibacterial composition, comprising:

[0086] Components content Benzalkonium chloride 20mg Aprotinin 25000KIU Sodium azulenesulfonate 0.2mg Sodium chloride 40mg Aspartame 72mg Zinc gluconate 70mg Chrysanthemum Extract 6mg Dandelion extract 2mg purified water 20g

[0087] Preparation method:

[0088] (1) Take 90% purified water, add benzalkonium chloride, aprotinin, and sodium chloride in sequence, and stir to dissolve;

[0089] (2) Add liquid mint flavor, aspartame, sodium azulenesulfonate, and zinc gluconate in sequence and stir to dissolve;

[0090] (3) Add chrysanthemum extract and honeysuckle extract in sequence, add the remaining purified water after dissolving; stir evenly, filter with an MCE filter membrane with a pore size of 0.8 μm, and the filtrate is the antiviral and antibacterial composition;

[0091] (4) Fill into oral liquid medicinal high-density polyethylene bottle + spray pump to obtain antiviral and antibacterial spray.

[0092] Example 8

[0093] The difference between this embodiment and embodiment 3 is that the amount of mint essence is halved.

[0094] Example 9

[0095] The difference between this example and Example 3 is that grape essence is used to replace half of the mint essence, and steviol glycoside (2000 μg / mL, calculated per unit volume of solvent water) is added.

[0096] Example 10

[0097] The difference between this embodiment and embodiment 3 is that aspartame is replaced by equal amounts of steviol glycosides and sucralose, and the ratio of stevioside to sucralose is 1:1.25.

[0098] Antiviral and antibacterial composition effect test

[0099] (1) Antibacterial effect test

[0100] According to the antibacterial performance test of dissolution antibacterial (inhibitory) products in GB 15979-2002, the antiviral and antibacterial composition prepared in Example 3 was used to test its antibacterial effect on Escherichia coli, Staphylococcus aureus, and Candida albicans; the number of viable bacteria and the antibacterial rate in the samples after the antiviral and antibacterial composition was exposed to the solution for 2 minutes, 5 minutes, 10 minutes, and 20 minutes were recorded. Each test was repeated three times in parallel. The results are as follows:

[0101]

[0102] After three repeated tests in each group, the antiviral and antibacterial composition prepared in Example 3 had an inhibition rate of more than 99% against Escherichia coli, Staphylococcus aureus and Candida albicans when acting for 2 min, 5 min, 10 min and 20 min, respectively, and had a strong antibacterial effect.

[0103] The antibacterial effect of the antiviral and antibacterial composition prepared in Example 3 on Pseudomonas aeruginosa was determined by the suspension quantitative antibacterial test in WS-T 650-2019 "Antibacterial and Antibacterial Effect Evaluation Method"; the number of viable bacteria and the antibacterial rate in the sample after 2 minutes, 5 minutes, 10 minutes, and 20 minutes of the antiviral and antibacterial composition were recorded, and the test was repeated 3 times in parallel; the test results are as follows:

[0104]

[0105] After three repeated tests, the antiviral and antibacterial composition prepared in Example 3 had an antibacterial rate of more than 99% against Pseudomonas aeruginosa after being treated for 2 min, 5 min, 10 min, and 20 min, respectively, and had a strong antibacterial effect against Pseudomonas aeruginosa.

[0106] In summary, the antiviral and antibacterial composition of the present invention has an antibacterial rate of more than 99% against Escherichia coli, Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa, and has a strong antibacterial effect.

[0107] (2) Product irritation test

[0108] Sample: the antiviral and antibacterial composition prepared in Example 3;

[0109] Animal species: New Zealand White rabbit (Source: Shanghai Songjiang District Chedun Experimental Animal Breeding Farm Co., Ltd., production license number: SCXK (Shanghai) 2022-0001);

[0110] Test method:

[0111] Both eyes of New Zealand rabbits were examined within 24 hours before the start of the experiment (including examination with sodium fluorescein). Animals with signs of eye irritation, corneal defects and conjunctival damage were not used in the experiment.

[0112] 0.1 mL of the test substance was instilled into the conjunctiva of the left eye of a New Zealand white rabbit. The right eye was instilled with normal saline as a normal control. After instillation, the eye was passively closed for 4 seconds and then rinsed with normal saline after 30 seconds.

[0113] Clinical Examination: One hour, 24 hours, 48 ​​hours, and 72 hours after eye instillation, the conjunctiva, iris, and cornea of ​​the New Zealand rabbits were visually inspected for damage and recovery. No irritation was observed within 72 hours, so the study was terminated. After the 24-hour observation and recording period, all animals' eyes were further examined with 2% sodium fluorescein solution.

[0114] Judgment criteria and scoring: The acute irritation reactions of the cornea, iris and conjunctiva of the New Zealand rabbits were scored according to the scoring criteria for acute eye irritation reactions of domestic rabbits, and the scores of corneal damage, iris damage, conjunctival congestion and conjunctival edema were calculated for each animal at three different observation times (24h, 48h and 72h).

[0115] The average scores and recovery times of corneal, iris, and conjunctival congestion and edema were used to determine the eye irritation intensity of the test substance according to the eye irritation reaction grading standards in Table 2-14 and Table 2-15 of 2.3.4.4 of the "Technical Specifications for Disinfection" (2002 Edition). The specific test results are shown in the following table:

[0116]

[0117] The above eye irritation results show that the antiviral and antibacterial composition of the present invention, when applied to New Zealand white rabbits, scored 0 for acute eye irritation, as did the control group treated with normal saline solution, indicating a non-irritating effect. This means that the antiviral and antibacterial composition of the present invention, when applied as a spray, is non-irritating and non-corrosive to the eyes if accidentally introduced into the eyes, resulting in no side effects such as eye damage.

[0118] (III) Product virus inactivation test

[0119] 1. Equipment, Raw Materials and Testing Methods

[0120] Sample information: The antiviral and antibacterial composition in Example 3 was used as the test substance

[0121] Virus Name: H1N1 and H3N2

[0122] Host cell: MDCK

[0123] Culture medium: DMEM / AJ30750638

[0124] Neutralizer: D / E neutralizing broth

[0125] Instruments and equipment: CO2 incubator BPN-CRH (WPE-RH0274), inverted microscope LWD300-38LT (WPE-RH0164), biological safety cabinet BSC-1604ⅡB2 (WPE-RH0313)

[0126] Testing basis: Technical Specifications for Disinfection (2002 edition) 2.1.1.10

[0127] Testing environment: Temperature: 22.9°C, Humidity: 51%RH

[0128] Virus extinguishing test: The test substance is exposed to the fire for 30 minutes. The test is repeated 3 times.

[0129] 2. H3N2 influenza virus test results

[0130] 1. The results of the H3N2 influenza virus neutralizer identification test are shown in the table below:

[0131] Results of the H3N2 influenza virus neutralizer identification test

[0132]

[0133] 2. The results of the H3N2 influenza virus inactivation test are as follows:

[0134]

[0135] The antiviral and antibacterial composition has an inactivation logarithmic value of >4.00 for H3N2 influenza virus in 30 minutes, and a virus inactivation rate of greater than 99.99%.

[0136] 3. H1N1 influenza virus test results

[0137] 1. The results of the H1N1 influenza virus neutralizer identification test are shown in the table below:

[0138]

[0139] 2. The results of the H1N1 influenza virus inactivation test are as follows:

[0140]

[0141] After testing, the antiviral and antibacterial composition had an inactivation logarithmic value of >4.00 for H1N1 influenza virus in 30 minutes, and an inactivation rate of over 99.99%.

[0142] The present application also conducted the above-mentioned tests on the antiviral and antibacterial compositions obtained in Examples 1 and 2. The antibacterial rates of the antiviral and antibacterial compositions in Examples 1 and 2 against Escherichia coli, Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa were all higher than 99%, showing strong antibacterial effects; and they were non-irritating and non-corrosive to the eyes; the obtained antiviral and antibacterial compositions had an inactivation rate of up to 99.99% against H3N2 influenza virus and H1N1 influenza virus in 30 minutes, showing good virus inactivation effect.

[0143] (IV) Product taste test

[0144] The mouthfeel of the antiviral and antibacterial composition sprays obtained in Examples 1-10 after being sprayed into the oral cavity was tested respectively;

[0145] Testing method: Five subjects were used to conduct a taste test. The taste level was designed to be divided into 4 levels: bitter at the beginning and bitter aftertaste was scored as 1; sweet at the beginning and bitter aftertaste was scored as 2; relatively sweet at the beginning and relatively bitter aftertaste was scored as 3; sweet at the beginning and relatively sweet aftertaste was scored as 4. The results are shown below:

[0146] Example Subject 1 Subject 2 Subject 3 Subject 4 Subject 5 Example 1 4 4 4 4 4 Example 2 4 4 4 4 4 Example 3 4 4 4 4 4 Example 4 1 1 1 1 1 Example 5 1 1 1 1 1 Example 6 2 2 2 2 2 Example 7 2 2 2 2 2 Example 8 4 4 3 4 3 Example 9 3 4 4 4 4 Example 10 2 2 2 2 2

[0147] The above taste test results show that the antiviral and antibacterial composition obtained by the experimental scheme of the present application is not only sweet in the mouth, but also relatively sweet. The composition containing mint flavor and aspartame (Examples 8-9) also has a good taste, and the use of other flavoring agents cannot mask the bitter taste of the composition of the present invention.

[0148] In the present application, the obtained antiviral and antibacterial composition was placed in a constant temperature box at 60°C for 5 hours. The taste of the antiviral and antibacterial compositions obtained in Examples 1-3 did not change significantly. The compositions in Examples 8 and 9 had a bitter taste, while the bitter taste of the compositions in Examples 4-7 and Example 10 was aggravated. It can be seen that the antiviral and antibacterial composition in the present application has better durability and stability.

Claims

1. An antiviral and antibacterial composition, characterized in that: It contains the following ingredients: aprotinin, benzalkonium chloride, and zinc gluconate.

2. The antiviral and antibacterial composition according to claim 1, characterized in that The antiviral and antibacterial composition also includes a solvent. Calculated per unit volume of the solvent, the antiviral and antibacterial composition includes the following components: aprotinin 800 KIU / mL~1500 KIU / mL, benzalkonium chloride 800 μg / mL~1200 μg / mL, zinc gluconate 3200 μg / mL~3800 μg / mL, plant extract 250 μg / mL-650 μg / mL, and flavoring agent 2300-9000 μg / mL.

3. The antiviral and antibacterial composition according to claim 1, characterized in that The antiviral and antibacterial composition further comprises a flavoring agent, which comprises mint essence and aspartame. The antiviral and antibacterial composition contains 2000-5000 μg / mL of mint essence and 300-4000 μg / mL of aspartame, calculated per unit volume of solvent.

4. The antiviral and antibacterial composition according to claim 1, characterized in that The invention also includes plant extracts, which include chrysanthemum extract and dandelion extract. The antiviral and antibacterial composition contains 200 μg / mL-500 μg / mL of chrysanthemum extract and 50 μg / mL-150 μg / mL of dandelion extract, calculated per unit volume of solvent.

5. The antiviral and antibacterial composition according to claim 1, characterized in that The antiviral and antibacterial composition further comprises sodium azulene sulfonate, wherein the sodium azulene sulfonate content is 5 μg / mL-15 μg / mL, calculated per unit volume of solvent.

6. The antiviral and antibacterial composition according to claim 1, characterized in that Also included are isotonic agents, which include sodium chloride.

7. The antiviral and antibacterial composition according to claim 1, characterized in that Calculated per unit volume of solvent, the invention includes benzalkonium chloride 800μg / mL~1200μg / mL, zinc gluconate 3200μg / mL~3800μg / mL, chrysanthemum extract 200μg / mL-500μg / mL, dandelion extract 50μg / mL-150μg / mL, aprotinin 800KIU / mL~1500KIU / mL, sodium azulenesulfonate 5μg / mL-15μg / mL, aspartame 400-900μg / mL, mint flavor 2500-3500μg / mL, and sodium chloride 1500-2500μg / mL.

8. The antiviral and antibacterial composition according to claim 1, characterized in that Calculated per unit volume of solvent, it includes benzalkonium chloride 1000 μg / mL, zinc gluconate 3500 μg / mL, chrysanthemum extract 300 μg / mL, dandelion extract 100 μg / mL, aprotinin 1250 KIU / mL, sodium azulenesulfonate 10 μg / mL, aspartame 600 μg / mL, mint flavor 3000 μg / mL, and sodium chloride 2000 μg / mL.

9. The method for preparing the antiviral and antibacterial composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: Take 70%-90% of the total purified water, add the remaining components in sequence, stir and dissolve; then add the remaining purified water, mix and filter, and the filtrate is the antiviral and antibacterial composition.

10. Use of the antiviral and antibacterial composition according to any one of claims 1 to 8.

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