Efficient seedling forming method for myricaria laxiflora seedlings

Abstract

The invention discloses an efficient seedling formation method for myricaria laxiflora seedlings. The method comprises the following steps: mixing peat nutrient soil with sand to prepare a bud matrix; spreading the selected seeds in a seedling raising tray, keeping wet, culturing in a greenhouse until the seeds germinate and plants grow to 3cm, culturing the seedling raising tray at room temperature and under a full-spectrum quantum plate, keeping the soil wet, and culturing; after branches and stems are lignified, seedlings are transplanted into flowerpots with peat mixture matrixes, the full-spectrum cultivation environment of the greenhouse is kept for cultivation, in the process, seedlings poor in growth vigor are pruned and irrigated with clear water and 1 / 2 MS nutrient solution, and the seedlings can be transplanted when growing to 20-30 cm. According to the method, seedlings are successfully bred through seeds for the first time, and seedling plants with branches and lignification are cultured; a common LED lamp and a full-spectrum quantum plate are adopted for seedling raising, obtained grown-up seedling plants are developed in root system, luxuriant in branches and leaves, lignified in stems and straight and upright, the seedling rate of seedlings is remarkably increased, and the method is suitable for large-scale field transplanting.

Description

Technical Field

[0001] The invention belongs to the technical field of biological propagation, and specifically discloses a high-efficiency seedling-forming method for Cyperus sparsely flowered seedlings. Background Art

[0002] Myricaria laxiflora is a shrub of the genus Myricaria in the family Tamarix, native to the floodplains of the middle reaches of the Yangtze River. It is mainly distributed in the drawdown zone of the Three Gorges Reservoir, which was originally 70-155m above sea level. The construction of the Three Gorges Project caused the water level to rise, submerging its core habitat in the reservoir and making it an endangered plant. In 2021, Myricaria laxiflora was listed as a second-class protected plant in the "National List of Key Protected Wild Plants". The main distribution areas of its current discovery are concentrated in Sichuan, Chongqing, and Hubei in the Yangtze River Basin. Including Yibin, Luzhou, and Leshan in Sichuan, Wanzhou and Fuling in Chongqing, and Yichang, Badong, and Zigui in Hubei. The branches and leaves of Myricaria laxiflora are lanceolate or oblong, densely growing on the green twigs of the current year. The racemes are usually terminal, the capsules are narrowly conical, and the root system is well developed. Through its long-term adaptation to the environment, it has developed a unique ability to withstand flooding, maintaining its leaves in winter and falling dormant after flooding in summer. Its strong resistance to flooding and erosion makes it suitable for slope consolidation and sand protection in riverbanks, and it is also used as a folk medicinal herb and has a certain ornamental value.

[0003] Although Eupatorium sparseflorum has a high seed germination rate, artificially propagated seedlings exhibit developmental impairments under standard lighting conditions, manifesting as phenotypic abnormalities such as branch loss, non-lignified stems, and prostrate growth. This results in the inability to establish normal plants, hindering their potential for reintroduction into the wild. Current research on Eupatorium sparseflorum propagation focuses primarily on asexual propagation, such as tissue culture, air layering, and cuttings. However, no research has been reported on seed propagation to seedling stage, either domestically or internationally. Asexual propagation requires collecting cuttings from native populations, which has a significant impact on the development of wild populations. Furthermore, asexually propagated plants lack a true taproot, primarily relying on fibrous roots, resulting in a shallow, weak root system. Wild reintroduction plantings are easily washed away during flood seasons, resulting in unstable growth. Furthermore, asexually propagated offspring do not undergo genetic recombination, retaining only the original traits and failing to inherit genes for other desirable traits within the population. This results in a limited genetic diversity, reducing their ability to adapt to the environment. When grown from seedlings of the sparsely-flowered water chestnut, the plantlets possess a well-developed taproot system, making them more resilient to flooding than vegetatively propagated plants. This makes them valuable for ecological restoration and slope protection along the Yangtze River. Furthermore, the rich genetic diversity of the seedling offspring is of great significance for population expansion, adaptive evolution research, and long-term conservation of the species.

[0004] Based on the above analysis, an efficient seedling-growing method for thinning out the seedlings of Cyperus rotundus is urgently needed in the industry. Summary of the Invention

[0005] To address these issues, the present invention provides a highly efficient artificial seedling method for the endangered plant Eupatorium sparsely flowered. Mature seeds are selected, germinated in a greenhouse, and seedlings are obtained. These seedlings are then grown under full-spectrum light and transplanted to produce mature plants with branched, lignified stems. This method, a sexual reproduction method, allows Eupatorium sparsely flowered to possess high genetic diversity and strong adaptability.

[0006] In order to achieve the above technical objectives, the present invention adopts the following technical means:

[0007] An efficient seedling-raising method for thinning-flowered cypress seedlings comprises:

[0008] (1) Spread the selected seeds in a seedling tray filled with a specific germination medium, cover with plastic wrap, place in a water tray, move into a greenhouse, cultivate until the seedlings emerge, and then culture under a weak light source;

[0009] (2) When the plant is 3-5 cm tall, keep the soil moist and place it in a greenhouse under a full-spectrum quantum board;

[0010] (3) After the plants have branches and the stems have lignified, they are transplanted into flower pots and cultivated with peat mixture in a greenhouse under full-spectrum quantum boards. The soil moisture is maintained and nutrient solution is sprayed after each watering. During this period, the growth of the plants is observed. The branches and leaves that grow poorly can be cut. Later, branch seedlings will be sent out, and more lush and robust seedlings will be obtained. When the plants grow to a height of 15±2 cm, they are moved to outdoor cultivation. The selected outdoor location needs to be exposed to direct sunlight.

[0011] (4) When the seedlings grow to 15-25 weeks, you can get 25-40 cm tall seedlings of sparse-flowered water cypress, which can be transplanted into the wild at this time.

[0012] Furthermore, the seeds selected in step (1) are: yellow seeds covered with white soft hairs and sticky on the capsules that have just cracked on the branches of the sparse-flowered cypress.

[0013] Furthermore, the specific germination matrix in step (1) is: peat and Yangtze River sand are mixed in equal volumes, moistened with clean water, placed in a seedling tray with a mesh structure at the bottom, and slowly irrigated with clean water to completely soak the soil.

[0014] Furthermore, the cultivation in step (1) needs to be covered with plastic wrap to keep moisture before the seedlings emerge, and the greenhouse temperature is controlled at 25°C.

[0015] Furthermore, the weak light source in step (1) includes: a common LED light source or a full-spectrum light source; the light intensity of the weak light source is less than 5000 lux, the illumination time is 12 hours per day, and the culture is carried out at 22-28°C for 10-20 days. Using the quantum board can cause the plant to branch and grow, the stem begins to lignify, and grow upright.

[0016] Furthermore, the greenhouse temperature in step (2) is 18-28°C; the wavelength range of the light source under the full-spectrum quantum board is 400-750nm, the light intensity is 35000-45000 lux, and the illumination time is 12h / d.

[0017] Furthermore, the specifications of the flower pots for transplanting in step (3) are: flower pots or flower pots with a height of 15 to 20 cm and a diameter of 10 to 15 cm, which are beneficial to the growth of plant roots; and the peat mixture includes equal volumes of peat and Yangtze River sand.

[0018] Furthermore, the nutrient solution in step (3) is 1 / 2MS basic nutrient solution.

[0019] Furthermore, the greenhouse temperature in step (3) is 18-28°C; the wavelength range of the light source under the full-spectrum quantum board is 400-750nm, the light intensity is 35000-45000 lux, and the illumination time is 12h / d.

[0020] The beneficial effects of the present invention are:

[0021] Euphrasia sparsely flowered water chestnut is one of the wild plants with extremely small populations in the upper reaches of the Yangtze River in my country, and has extremely high academic and application value. In view of the endangered nature of this species, expanding the population through artificial breeding of seed seedlings is one of the important ways to effectively protect this species. The native habitat of Euphrasia sparsely flowered water chestnut is the ebb and flow zone of the Yangtze River, which has strict requirements on water level fluctuations, soil matrix and light conditions. Artificially cultivated seedlings have weak flooding resistance and insufficient stress resistance in the early stage of transplanting. In addition, long-term monitoring by a professional management team is required for their return to the wild to prevent pests and diseases and human damage. Based on this, the present invention cultivates seed seedlings of Euphrasia sparsely flowered water chestnut, which provides important scientific basis and technical means for the conservation biology, restoration ecology, population return to nature and development and utilization of resources of this species. Directly using seeds of the sparse-flowered water cypress tree for rapid and efficient artificial propagation of species seedlings, the full spectrum, high-intensity light treatment promotes branch growth and stem lignification, resulting in an upright plant shape. This overcomes the difficulty of traditional seedling cultivation methods in which seedlings cannot normally achieve branch growth and stem lignification and upright growth, providing technical support for the artificial propagation, population recovery, and effective resource protection of this rare and endangered species. This invention successfully propagates seedlings from seeds for the first time and cultivates branched and lignified mature plants; seedling cultivation uses ordinary LED lights and full-spectrum quantum panels, resulting in mature plants with well-developed root systems, lush branches and leaves, and lignified and upright stems, significantly improving the seedling success rate and making them suitable for large-scale field transplanting. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a physical picture of the germination matrix configured by the present invention;

[0023] Figure 2 The present invention is a physical picture of seedlings obtained after the seeds are cultured in a germination medium for 15 days;

[0024] Figure 3 The present invention is a physical picture showing abnormal growth of seedlings after about 60 days of cultivation under weak light;

[0025] Figure 4 The present invention is a physical picture of the normal development of seedlings after being cultured under full-spectrum strong light for about 60 days;

[0026] Figure 5 This is a photo of the seedlings of the present invention grown after about 100 days of indoor cultivation after transplanting;

[0027] Figure 6 This is a photo of the seedlings (including roots) of the present invention after being transplanted and cultured indoors for about 100 days;

[0028] Figure 7 This is a photo of the seedlings of the present invention grown into seedlings after being transplanted and cultured outdoors in the sun for about 100 days. DETAILED DESCRIPTION

[0029] The present invention is further described below with reference to examples, but the scope of the present invention is not limited to the scope of the examples.

[0030] Example 1:

[0031] (1) Seed collection: Around November, we collected the newly cracked capsules from the branches of the sparse-flowered cypress distributed along the Yangtze River. The capsules were covered with white soft hairs and had sticky yellow seeds. We placed them in kraft paper and brought them back to the laboratory.

[0032] (2) Germination substrate configuration: PINDSTRUP peat and Yangtze River sand are mixed with water in a ratio of 1:1, and then moved into a seedling tray with a mesh structure at the bottom and completely soaked with clean water ( Figure 1 ).

[0033] (3) Germination: Sprinkle the collected seeds evenly onto the surface of the germination matrix seedling tray, cover with plastic wrap to keep moist before germination, and place the seedlings under ordinary LED light source for cultivation. The light time is 12h / d, and the seedlings are cultured in a greenhouse at 22-28℃ for about 15-20 days. The seed germination rate can reach more than 90% ( Figure 2 ).

[0034] (4) Seedling strengthening process: When the seedlings are 3-5cm tall, keep the soil moist and place them in a greenhouse at 18-28℃ under a full-spectrum quantum board. The wavelength range of the light source is 400-750nm, the light intensity is 35,000-45,000 lux, and the lighting time is 12h / d. After being cultured under full-spectrum strong light, the branches of the cypress will be able to grow, the stems will begin to lignify, and the plants will grow upright. Otherwise, they will not develop branches and can only grow prostrate. Figure 3 ).

[0035] (5) Transplantation: Wait until the seedlings grow branches and the stems become lignified ( Figure 4 ), transplant to a flower pot / pot with a height of about 15 cm and a width / diameter of 10-15 cm. The cultivation soil is still prepared with a ratio of peat and Yangtze River sand in a ratio of 1:1. The cultivation conditions are the same as (4). Daily irrigation is used to maintain soil moisture. After each irrigation, 1 / 2MS basic nutrient solution is sprayed. The survival rate of seedlings after transplanting can reach more than 98%. After the seedlings grow for 15-25 weeks, sparse-flowered water cypress seedlings with a height of 20-30 cm can be obtained ( Figure 5 、 6 During this period, observe the growth of the plants and prune any poorly growing branches and leaves so that they can subsequently develop into high-quality plants. After transplanting, the plants have greater spacing, more soil, and better nutrition, which is more conducive to plant growth and development.

[0036] Example 2:

[0037] Based on Example 1, in step (3), after emergence, the seedlings can also be placed under a full-spectrum quantum dot plate, with a light intensity of less than 5000 lux, a light duration of 12 hours per day, and cultured in a 22-28°C greenhouse for about 10-20 days, with other conditions remaining unchanged. The seed emergence rate can reach over 90%, but the growth rate will be faster.

[0038] Example 3:

[0039] On the basis of Example 1, the transplanted plants grow to a height of about 15 cm. After the spring, the flower pots can be moved to the outdoors in direct sunlight for cultivation. The management and maintenance are carried out according to the weather conditions. The rest remains unchanged. After the seedlings grow for 15-25 weeks, the seedlings of 25-40 cm tall water cypress can be obtained ( Figure 7 ).

[0040] Artificial cultivation of Eupatorium sparsely flowered has moved from the technical exploration stage to large-scale application, but maintaining genetic diversity, improving the adaptability of seedlings in the field, and cost control remain major challenges. The seedlings cultivated using the specific method of the present invention have excellent growth and a high survival rate. The mature plants cultivated by this method can provide an effective method for the large-scale propagation of Eupatorium sparsely flowered, thereby achieving better species protection in the future and enhancing its adaptability to the environment.

Claims

1. A method for efficiently raising seedlings of Cyperus sparsely planted seedlings, comprising: (1) Spread the selected seeds in a seedling tray filled with a specific germination medium, cover with plastic wrap, place in a water tray, move into a greenhouse, and culture under a weak light source after germination; (2) When the plant is 3-5 cm tall, keep the soil moist and place it in a greenhouse under strong light from a full-spectrum quantum dot; (3) After the plants have branches and the stems have lignified, they are transplanted into flower pots and cultivated with peat mixture. They are placed in a greenhouse and cultivated under strong light from a full-spectrum quantum board. The soil moisture is maintained and nutrient solution is sprayed after each watering. During this period, the growth of the plants is observed and any branches and leaves that grow poorly can be cut. When the plants grow to a height of 15 ± 2 cm, they are moved outdoors for cultivation. (4) When the seedlings grow to 15-25 weeks, you can get 25-40 cm tall seedlings of sparse-flowered water cypress, which can be transplanted into the wild at this time.

2. The efficient seedling-forming method according to claim 1, wherein: The seeds selected in step (1) are: capsules that have just cracked on branches of cypress, yellow seeds covered with white soft hairs; the specific germination matrix is: peat and Yangtze River sand mixed in equal volumes.

3. The efficient seedling-forming method according to claim 1, wherein: The step (1) of cultivating the seedlings needs to be covered with plastic wrap to keep them moist.

4. The efficient seedling-forming method according to claim 1, wherein: The weak light source in step (1) includes: a common LED light source or a full-spectrum light source; The illumination intensity of the weak light source is below 5000 lux, the illumination time is 12 h / d, and the culture is carried out at 22-28° C. for 10-20 days.

5. The efficient seedling-forming method according to claim 1, wherein: The temperature of the greenhouse in step (2) is 18-28°C; The wavelength range of the light source under the full-spectrum quantum board is 400-750nm, the light intensity is 35000-45000 lux, and the illumination time is 12h / d.

6. The efficient seedling-forming method according to claim 1, wherein: The specifications of the flower pots in step (3) are: a flower pot with a height of 15 to 20 cm and a diameter of 10 to 15 cm; The peat mixture includes equal volumes of peat and Yangtze River sand.

7. The efficient seedling-forming method according to claim 1, wherein: The nutrient solution in step (3) is 1 / 2MS basic nutrient solution.

8. The efficient seedling-forming method according to claim 1, wherein: The temperature of the greenhouse in step (3) is 18-28°C; The wavelength range of the light source under the full-spectrum quantum board is 400-750nm, the light intensity is 35000-45000 lux, and the illumination time is 12h / d.

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