Intelligence promoting and nerve calming mushroom soup formula based on gastrodia elata-edible mushroom synergistic interaction

Abstract

The invention relates to the technical field of functional food, and particularly discloses a gastrodia elata-edible mushroom synergistic interaction-based intelligence-promoting and nerve-soothing mushroom soup formula which comprises seven raw materials including morchella esculenta (3-5 cm), pleurotus citrinopileatus (the umbrella diameter is 4-7 cm), pleurotus geesteranus (pileus is 2-4 cm / stem is smaller than or equal to 1 cm), gastrodia elata (2 cm thick slices), velvet antler mushrooms (4-5 cm segments), shiitake mushrooms (the umbrella diameter is 4-6 cm) and bolete (the slice thickness is 0.5-1 mm). The gastrodin-beta glucan compound is formed and a neurotransmitter dual-channel regulation mechanism is constructed by setting dynamic proportion conservation of raw materials to maintain size-activity matching, combining gradient superfine grinding with cryogenic wall breaking to directionally release active ingredients and activating a pleated channel through enzyme-ultrasonic coupling, so that the efficacy stability and brain targeting are remarkably improved, and the application prospect is wide. The composition is suitable for improving cognition and sleep functions.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and more particularly to a formula of an intelligence-enhancing and mind-calming mushroom soup based on the synergistic enhancement of gastrodia elata and edible fungi. Background Art

[0002] In recent years, the development of brain health functional foods has focused on the combination of Gastrodia elata and edible fungi. Traditional approaches often involve simply combining Gastrodia elata extracts with conventional mushrooms (such as Lentinus edodes and Ganoderma lucidum). Although basic research has confirmed the neuromodulatory potential of gastrodin and fungal polysaccharides, three bottlenecks remain: a lack of precise control over raw material combinations, inefficient active ingredient delivery, and a mismatch between dosage forms and circadian rhythms. This results in unstable product efficacy and poor clinical reproducibility, and existing technologies still have certain limitations: 1. Ambiguous raw material specifications weaken the basis for collaboration The existing patent only defines the type of fungus, and fails to recognize that the polypeptide content of the 3-5cm fruiting body of Morchella geesteranus is 40%-45% higher than that of other specifications. It also ignores the 35%-38% increase in glutamate release rate when the stipe of Pleurotus geesteranus is ≤1cm, resulting in efficacy fluctuations of >30% between batches.

[0003] 2. Processing technology destroys the structure of active ingredients Conventional decoction methods result in a thermal degradation rate of gastrodin exceeding 32% (thermogravimetric analysis confirms a weight loss peak at 220°C), and the molecular weight of edible mushroom β-glucan degrades from 50 kDa to less than 10 kDa. In particular, the blood-brain barrier permeability is less than 15% (in an in vitro hCMEC / D3 cell model), and the boletus terpene dissolution rate is less than 50%, making it impossible to form an effective complex.

[0004] 3. Dosage form design ignores neural regulation rhythm Commercially available dosage forms do not differentiate between day and night needs: the rehydration time of instant powder is >60 seconds, resulting in oxidative loss >20%; the soft capsules lack brain-targeting design, and the oral bioavailability is only 55%-60% (human AUC0-∞ test); the lack of nighttime sustained-release dosage forms leads to a sharp drop in sleep intervention effect by >40%.

[0005] Therefore, in order to solve the above problems, a recipe of intelligence-enhancing and mind-calming mushroom soup based on the synergistic enhancement of Gastrodia elata and edible fungi was proposed. Summary of the Invention

[0006] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present invention provide a recipe for an intelligence-enhancing and mind-calming mushroom soup based on the synergistic enhancement of Gastrodia elata and edible fungi to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a formula for a brain-enhancing and mind-soothing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi, which is composed of the following precise components and physical-biochemical dual specification indicators: Dried fruiting bodies of Morchella edodes, length 3.0 to 5.0 cm, dosage 4.0 g ± 0.2 g; Whole golden oyster mushrooms of standard grade, with a cap diameter of 4.0 to 7.0 cm and an intact stem, are used at a dosage of 12.0 g ± 0.5 g. For grade B or C selected Pleurotus geesteranus, with a cap diameter of 2.0 to 4.0 cm and a stipe length ≤ 1.0 cm, the dosage is 18.0 g ± 0.8 g. Gastrodia elata slices, thickness 2.0 ± 0.1 cm and retaining the ring-shaped horizontal structure, dosage 12.0 g ± 0.6 g; A middle segment of velvet antler mushroom, 4.0 to 5.0 cm in length and with radial fibrous structures on the cross section, is used in an amount of 15.0 g ± 0.7 g. Common-grade dried shiitake mushrooms, with a cap diameter of 4.0 to 6.0 cm and a flesh thickness of 2.0 mm or more, are used in a dosage of 18.5 g ± 0.9 g. Grade B dried slices of porcini mushrooms, 0.5 to 1.0 mm thick and free of insect-eaten black spots, dosage 5.5 g ± 0.3 g.

[0008] Preferably, the fluctuation of component dosage follows the principle of dynamic proportion conservation: when the actual dosage of any raw material exceeds the benchmark value, the remaining raw materials will increase according to their mass percentage year-on-year; when the actual dosage of any raw material is lower than the benchmark value, the remaining raw materials will decrease year-on-year; the deviation of the total mass relative to the benchmark value of 84.0 grams does not exceed ±3.0%, and the size ratio of the length of the morel to the thickness of the Gastrodia elata slice is maintained constant (3.0-5.0):2.0.

[0009] Preferably, the Gastrodia elata slices are subjected to gradient ultrafine grinding: 200 mesh, 250 mesh, and 300 mesh are sequentially ground under liquid nitrogen protection, the cavity temperature is ≤40°C, and the final particle size is ≤48 microns.

[0010] Preferably, the morels and boletus are subjected to a deep-cold cell wall breaking treatment: three cycles of impact crushing are performed using 0.6 MPa compressed air in an environment of -18°C ± 1°C, so that the cell wall breaking rate is ≥ 90%.

[0011] Preferably, the velvet antler mushroom and the Pleurotus geesteranus are treated by biological enzymatic hydrolysis-ultrasound coupling: 0.5 enzyme activity units of cellulase per gram of raw material are added, enzymatic hydrolysis is carried out at 50°C±1°C for 1 hour, and then immediately transferred to a 40 kHz ultrasonic field for treatment for 20 minutes.

[0012] Preferably, the synergistic enhancement mechanism includes a gastrodin-β-glucan complex and a dual neurotransmitter balance pathway, wherein the gastrodin-β-glucan complex is formed by ultrafinely crushing gastrodin and edible fungus β-glucan to form a complex with a molecular weight of 5000-8000 Daltons; in the dual neurotransmitter balance pathway, morel polypeptides inhibit excessive activation of glutamate receptors, and the enzymatic hydrolysis products of velvet antler mushroom enhance the sensitivity of γ-aminobutyric acid receptors.

[0013] The technical effects and advantages of the present invention are as follows: 1. Structural compatibility of raw materials This formula establishes a structural synergy between the ingredients through precise matching of physical specifications and biochemical properties: For Morchella, the fruiting body is limited to 3-5cm to target the peptide-rich region; for Pleurotus geesteranus, the stipe is controlled to ≤1cm to ensure efficient glutamate release; and for Gastrodia elata, the slice thickness is fixed at 2cm to optimize the dissolution surface area. The size standards of each ingredient are directly linked to the release characteristics of its active ingredient, and a constant ratio of Morchella length to Gastrodia elata thickness is maintained to ensure synchronized dissolution kinetics of the neuroactive ingredients. This dual "form-function" adaptation design fundamentally avoids the ingredient fluctuations caused by the ambiguous specifications of traditional technologies.

[0014] 2. Targeted activation logic of the process chain To address the bottlenecks in active ingredient release, we employ a graded, targeted processing process. The circular, transverse structure of Gastrodia elata requires ultrafine grinding to expose terpene sites, so we employ gradient low-temperature crushing. The cell walls of edible mushrooms hinder peptide dissolution, so we employ a unique deep-cold airflow method to break the cell walls. Glutamate in the gills requires bio-enzymatic hydrolysis combined with physical field activation, so we devise an enzyme-ultrasound coupled release method. Each process is tailored to the physical barriers and thermal sensitivities of the raw materials, forming a progressive "cell wall breaking-release-activation" processing chain to maximize the retention of active ingredients and achieve targeted synergy enhancement.

[0015] 3. Functional complementarity of synergistic mechanisms The formula's inherent dual-pathway neuromodulatory mechanism demonstrates its functional rationality: Gastrodin and edible mushroom β-glucan form a complex through hydrophobic interactions, enhancing blood-brain barrier penetration; Morchella oleracea peptides inhibit glutamate receptor overexcitation, while antler antler enzymatic hydrolysate enhances GABA receptor sensitivity, creating an "inhibition-activation" neurotransmitter balance. This dual-stage synergy of "carrier transport + receptor regulation" achieves functional complementarity at the molecular level, forming a complete closed-loop action loop from ingredient delivery to physiological regulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a system framework diagram of the present invention. DETAILED DESCRIPTION

[0017] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0018] As attached Figure 1As shown, (1) the formula of the Yizhi Anshen Mushroom Soup based on the synergistic effect of Gastrodia elata and edible fungi is composed of the following precise components and physical-biochemical dual specification indicators: Dried fruiting bodies of Morchella edodes, length 3.0 to 5.0 cm, dosage 4.0 g ± 0.2 g; Whole golden oyster mushrooms of standard grade, with a cap diameter of 4.0 to 7.0 cm and an intact stem, are used at a dosage of 12.0 g ± 0.5 g. For grade B or C selected Pleurotus geesteranus, with a cap diameter of 2.0 to 4.0 cm and a stipe length ≤ 1.0 cm, the dosage is 18.0 g ± 0.8 g. Gastrodia elata slices, thickness 2.0 ± 0.1 cm and retaining the ring-shaped horizontal structure, dosage 12.0 g ± 0.6 g; A middle segment of velvet antler mushroom, 4.0 to 5.0 cm in length and with radial fibrous structures on the cross section, is used in an amount of 15.0 g ± 0.7 g. Common-grade dried shiitake mushrooms, with a cap diameter of 4.0 to 6.0 cm and a flesh thickness of 2.0 mm or more, are used in a dosage of 18.5 g ± 0.9 g. Grade B dried slices of boletus, 0.5 to 1.0 mm thick and free of insect-eaten black spots, are used in a dosage of 5.5 g ± 0.3 g. Among them, take the fruiting body of Morchella with a length of 3.0-5.0cm (measurement deviation of vernier caliper ±0.1mm) 4.0g±0.2g; the whole golden mushroom with a cap diameter of 4.0-7.0cm (drum screening sorting) 12.0g±0.5g; the Pleurotus geesteranus with a cap diameter of 2.0-4.0cm and a stem ≤1.0cm (image recognition sorting + automatic stem cutting) 18.0g±0.8g; the thickness of 2.0±0.1cm Hemp slices (laser thickness measurement to check for annular structure) 12.0g ± 0.6g; antler antler segments 4.0-5.0cm long (microscopic cross-section confirming ≥85% radial fibers) 15.0g ± 0.7g; shiitake mushrooms 4.0-6.0cm in diameter with ≥2.0mm flesh (puncture thickness measurement) 18.5g ± 0.9g; 0.5-1.0mm thick boletus without black spots (micrometer inspection at three locations) 5.5g ± 0.3g. Premix the seven ingredients in a nitrogen cabinet (oxygen concentration ≤ 5%) for 30 minutes.

[0019] (2) The fluctuation of component dosage follows the principle of dynamic proportion conservation: when the actual dosage of any raw material exceeds the benchmark value, the other raw materials will increase according to their mass percentage year-on-year; when the actual dosage of any raw material is lower than the benchmark value, the other raw materials will decrease year-on-year; the deviation of the total mass relative to the benchmark value of 84.0 g shall not exceed ±3.0%, and the size ratio of the length of Morchella edodes to the thickness of Gastrodia elata slices shall be maintained constant (3.0-5.0):2.0. Among them, when the actual weight of Morchella edodes is 4.2 g (+0.2 g), the system automatically increases the weight of Shiitake mushroom to 19.1 g according to the proportion coefficient (calculation formula: 18.5+(0.2 / 4.0)×18.5); if the actual weight of Boletus edulis is 5.2 g (-0.3 g), the weight of Pleurotus ostreatus will be decreased year-on-year to 14.6 g (15.0-(0.3 / 5.5)×15.0). The final total mass deviation is ≤±2.5% (baseline 84.0g), and the ratio of Morchella length / Gastrodia thickness is always (4.2:2.1)=2:1.

[0020] (3) Gastrodia elata slices were subjected to gradient ultrafine grinding: in a liquid nitrogen-protected environment, they were sequentially ground through three levels of 200 mesh, 250 mesh, and 300 mesh, with the chamber temperature ≤40°C and the final particle size ≤48 μm. The Gastrodia elata slices were placed in an STJ-300 ultrafine grinder, and the liquid nitrogen pipeline maintained the chamber temperature ≤40°C. They were then sequentially ground through three levels of 200 mesh (74 μm), 250 mesh (58 μm), and 300 mesh (48 μm). Samples were taken at intervals at each level and tested with a laser particle size analyzer. The final product had a D90 particle size of ≤45 μm (residual rate ≤2%), exposing the terpene binding sites in the annular striations.

[0021] (4) Cryogenic cell wall disruption of Morchella and Boletus: Three cycles of impact crushing were performed at -18°C ± 1°C using 0.6 MPa compressed air to achieve a cell wall rupture rate of ≥ 90%. Morchella and Boletus were placed in a CY-600 air flow mill, with liquid nitrogen spray at -18°C (± 1°C) and 0.6 MPa compressed air cycle crushing for 3 cycles of 120 seconds (with 30 seconds intervals for heat dissipation). Samples were taken and stained with methylene blue for microscopic examination (400×). The proportion of intact cells was ≤ 8%, and the intracellular polypeptide release rate was ≥ 92%.

[0022] (5) Bio-enzymatic hydrolysis-ultrasonic coupling treatment of velvet antler mushroom and Pleurotus geesteranus: add 0.5 enzyme activity units of cellulase per gram of raw material, enzymatic hydrolysis at 50℃±1℃ for 1 hour, and immediately transfer to 40 kHz ultrasonic field for 20 minutes. In which, velvet antler mushroom segments + Pleurotus geesteranus are added with pH 5.0 buffer (material-liquid ratio 1:15), and add 0.5U / g of cellulase (enzyme activity unit definition: hydrolyze microcrystalline cellulose to produce 1μmol glucose per minute at 50℃ / pH 5.0). After oscillation at 50℃±0.5℃ for 60 minutes, immediately transfer to 40kHz±1kHz ultrasonic tank (sound intensity meter calibrated power density 50W / L), and cycle for 10 seconds on / 2 seconds off for 20 minutes.

[0023] (6) The synergistic mechanism includes the gastrodin-β-glucan complex and the dual pathway of neurotransmitter balance. The gastrodin-β-glucan complex is formed by ultrafine grinding of gastrodin and edible fungus β-glucan to form a complex with a molecular weight of 5000-8000 Daltons; the dual pathway of neurotransmitter balance, Morchella peptide inhibits the over-activation of glutamate receptors, and the enzymatic hydrolysis product of Pleurotus ostreatus enhances the sensitivity of γ-aminobutyric acid receptors. Among them, 1g of gastrodin ultrafine powder and 2g of Quanyao 4 broken wall powder were taken, added with 50ml of distilled water, and shaken at 40℃ for 2 hours. The 5-8kDa component was separated by ultrafiltration. HPLC-MS showed that gastrodin (retention time 12.3min) and β-glucan (m / z 789.4) co-eluted, and the yield of the complex was 92.5%. After 28 days of gavage in SD rats, ELISA detection of cerebrospinal fluid GABA increased by 38.4±2.1%, and Western Blot showed that glutamate receptor expression decreased by 29.7±1.8%.

[0024] Implementation process of the present invention: Step 1: Raw material pretreatment 1.1 Deep-freeze cell wall breaking of morels and porcini Take 4.0g (±0.2g) of Morchella fruiting bodies with a length of 3.0-5.0cm and 5.5g (±0.3g) of dried Boletus slices with a thickness of 0.5-1.0mm and place them in a CY-600 low-temperature airflow mill: Ambient temperature: -18°C (liquid nitrogen injection temperature control ±1°C) Pressure: 0.6 MPa (accuracy 0.01 MPa) Cycle crushing: 3 times x 120 seconds (30 seconds interval for heat dissipation) End point determination: Methylene blue staining microscopy (400×), intact cells ≤ 8% 1.2 Gastrodia elata gradient ultrafine grinding Take 12.0g (±0.6g) of Gastrodia elata slices with a thickness of 2.0±0.1cm, pre-cool them in liquid nitrogen for 5 minutes, and then put them into the STJ-300 grinder: Stage screen mesh number target particle size duration temperature control Primary 200 mesh ≤74μm 2min ≤45℃ Intermediate 250 mesh ≤58μm 3min ≤42℃ Final grade 300 mesh ≤48μm 5min ≤40℃ End point: Residue rate after passing 300 mesh sieve ≤2%, laser particle size analyzer D90 ≤45μm 1.3 Pleurotus geesteranus / Pleurotus geesteranus enzyme-ultrasound coupling 15.0g (±0.7g) of velvet antler mushroom segments + 18.0g (±0.8g) of Pleurotus geesteranus, add pH 5.0 buffer (material-liquid ratio 1:15): Enzymatic hydrolysis: Cellulase 0.5 U / g (definition: hydrolyzes microcrystalline cellulose to produce 1 μmol of glucose per minute at 50°C / pH 5.0) Conditions: 50℃±0.5℃, 120 rpm shaking for 60 minutes Ultrasound: Immediately transfer to 40kHz±1kHz ultrasonic field (sound intensity meter calibrated power density 50W / L), work for 10 seconds / rest for 2 seconds, and treat for 20 minutes Step 2: Dynamic ratio control To enable the driven tolerance system: Case 1: Actual weight of morels: 4.2g (+0.2g) → Shiitake mushrooms: increased to 19.1g (calculation formula: 18.5 + (0.2 / 4.0) × 18.5) Case 2: Actual weight of porcini mushrooms: 5.2g (-0.3g) → Price of velvet antler mushrooms decreased to 14.6g (calculation formula: 15.0 - (0.3 / 5.5) × 15.0) Quality control: Total mass deviation ≤ ±2.5% (baseline 84.0g), constant Morchella length / Gastrodia thickness ratio (4.2cm:2.1cm=2:1) Step 3: Synergy Verification 3.1 Formation of Gastrodin-β-glucan Complex Take 1g of Gastrodia elata superfine powder (product 1.1.2) and 2g of broken mushroom powder (morels + boletus, product 1.1), add 50ml of distilled water, and shake at 40℃ for 2 hours: Ultrafiltration separation of 5-8 kDa fractions HPLC-MS detection: Gastrodin (retention time 12.3 min) and β-glucan (characteristic peak m / z 789.4) co-eluted, and the yield of the complex was ≥92.5%. 3.2 Dual neurotransmitter pathway regulation SD rat oral administration formula extract (human equivalent dose conversion): ELISA: CSF GABA concentration ↑38.4±2.1% after 28 days Western Blot: Glutamate receptor expression ↓29.7±1.8% Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense, and may refer to mechanical or electrical connections, internal communication between two components, or direct connection. "Up," "down," "left," and "right" are only used to indicate relative positional relationships. When the absolute positions of the objects being described change, the relative positional relationships may also change. Secondly: The drawings of the embodiments disclosed in the present invention only involve structures related to the embodiments disclosed in the present invention. Other structures may refer to conventional designs. The same embodiment and different embodiments of the present invention may be combined with each other without conflict. Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A formula for a brain-enhancing and mind-soothing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi, characterized in that: It is composed of the following precise components and physical and biochemical dual specifications: Dried fruiting bodies of Morchella edodes, length 3.0 to 5.0 cm, dosage 4.0 g ± 0.2 g; Whole golden oyster mushrooms of standard grade, with a cap diameter of 4.0 to 7.0 cm and an intact stem, are used at a dosage of 12.0 g ± 0.5 g. For grade B or C selected Pleurotus geesteranus, with a cap diameter of 2.0 to 4.0 cm and a stipe length ≤ 1.0 cm, the dosage is 18.0 g ± 0.8 g. Gastrodia elata slices, thickness 2.0 ± 0.1 cm and retaining the ring-shaped horizontal structure, dosage 12.0 g ± 0.6 g; A middle segment of velvet antler mushroom, 4.0 to 5.0 cm in length and with radial fibrous structures on the cross section, is used in an amount of 15.0 g ± 0.7 g. Common-grade dried shiitake mushrooms, with a cap diameter of 4.0 to 6.0 cm and a flesh thickness of 2.0 mm or more, are used in a dosage of 18.5 g ± 0.9 g. Grade B dried slices of porcini mushrooms, 0.5 to 1.0 mm thick and free of insect-eaten black spots, dosage 5.5 g ± 0.3 g.

2. The formula of the intelligence-enhancing and tranquilizing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi according to claim 1, characterized in that: The fluctuation of component dosage follows the principle of dynamic proportion conservation: when the actual dosage of any raw material exceeds the benchmark value, the remaining raw materials will increase according to their mass percentage year-on-year; when the actual dosage of any raw material is lower than the benchmark value, the remaining raw materials will decrease year-on-year. The deviation of the total mass relative to the benchmark value of 84.0 grams shall not exceed ±3.0%, and the size ratio of the length of the morel mushroom to the thickness of the Gastrodia elata slice shall be maintained constant (3.0-5.0):2.

0.

3. The formula of the intelligence-enhancing and mind-soothing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi according to claim 1, characterized in that: The Gastrodia elata slices were subjected to gradient ultrafine grinding: 200 mesh, 250 mesh and 300 mesh were sequentially ground under liquid nitrogen protection, the cavity temperature was ≤40°C, and the final particle size was ≤48 μm.

4. The formula of the intelligence-enhancing and tranquilizing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi according to claim 1, characterized in that: The morels and boletus were treated with deep freezing to break the cell wall: three cycles of impact crushing were performed using 0.6 MPa compressed air in an environment of -18℃±1℃ to achieve a cell wall breaking rate of ≥90%.

5. The formula of the intelligence-enhancing and tranquilizing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi according to claim 1, characterized in that: Porcupine antler mushroom and Pleurotus geesteranus were treated by biological enzymatic hydrolysis-ultrasound coupling: 0.5 enzyme activity units of cellulase per gram of raw material were added, and enzymatic hydrolysis was carried out at 50℃±1℃ for 1 hour, and then immediately transferred to a 40 kHz ultrasonic field for treatment for 20 minutes.

6. A formula of a brain-enhancing and mind-soothing mushroom soup based on the synergistic effect of Gastrodia elata and edible fungi according to any one of claims 3 to 5, characterized in that: The synergistic enhancement mechanism includes the gastrodin-β-glucan complex and the dual pathway of neurotransmitter balance. The gastrodin-β-glucan complex is a complex with a molecular weight of 5000-8000 Daltons formed by ultrafinely crushed gastrodin and edible fungus β-glucan; in the dual pathway of neurotransmitter balance, morel polypeptides inhibit the over-activation of glutamate receptors, and the enzymatic hydrolysis products of velvet antler mushroom enhance the sensitivity of γ-aminobutyric acid receptors.

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