Method for efficiently feeding sweet potato small weevil

Abstract

According to the method for efficiently feeding the small weevils of the sweet potatoes, the small weevils are fixed through the half-moon-shaped clamping rings, the small weevils are placed in a suspended mode, adult weevils are prevented from being squeezed to die, and the survival rate and the egg laying amount of the small weevils are remarkably increased. All parts of the device are detachable, easy to clean, recyclable, low in cost and convenient to operate. During potato block replacement, only adults need to be lightly brushed and integrally transferred, mechanical damage is reduced, and egg damage is avoided. The snap ring design facilitates marking and management and facilitates experiment tracking. Potato block burying and regular matrix cleaning promote smooth eclosion of larvae. The suspended structure enables adults to freely crawl, feed and lay eggs, distribution is uniform, and the utilization rate of potato blocks is increased. The method effectively solves the problems of imago death, uneven egg laying and larva extrusion caused by potato block accumulation in traditional feeding, greatly improves the feeding efficiency and reproductive performance, and has good application prospects.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of insect feeding, in particular to a method for efficiently feeding sweet potato weevils. BACKGROUND

[0002] The sweet potato weevil is also known as the sweet potato ant weevil, which mainly harms sweet potatoes and spinach convolvulus plants and is an important quarantine pest at home and abroad. The pest is small in size, has the characteristics of strong concealment, high reproduction rate, significant pseudo-death, overlapping generations, strong vitality, hidden occurrence and long adult lifespan. After the larvae hatch, they feed on the inside of the tuber or vine head, causing sudden and unpreventable damage. In recent years, due to the extensive planting and management mode of sweet potatoes, the sweet potato weevil has become more and more serious. At the same time, with the increasing attention of the sweet potato industry and the increasing frequency of seed potato trade, the pest continues to spread and spread, seriously restricting the safe production of sweet potatoes.

[0003] For a long time, the prevention and control of the sweet potato weevil in Zhejiang Province has been mainly based on chemical control, and relevant units at home and abroad have also carried out work related to the monitoring of insecticide resistance and the screening of control agents. These works require the development progress (development stage, development age, individual health) of the sweet potato weevil to be as consistent as possible. To achieve this requirement and ensure the accuracy of pesticide bioassay results, large-scale artificial feeding of test insects is necessary.

[0004] There are very limited documents related to the feeding of sweet potato weevils, and only the use of artificial feed for the feeding of sweet potato weevils is mentioned: first, the existing patent mentions a sweet potato weevil breeding and breeding device, including a breeding box, a pad, a guide frame, a transparent observation window, a buffer device, a positioning cylinder, a buffer spring, a limiting sheet, a supporting rod, a buffer plate, a locking mechanism, a spring box, a locking rod, a pull rod, a push piece, a locking spring, a light shield, a handle, and a protective plate. The above-mentioned feeding device is extremely complex, and the operation details are extremely tedious. The indoor artificial feeding of sweet potato weevils is complex and difficult to feed, which not only consumes a lot of time, but also causes the host plants (such as tubers and leaves) to rot and deteriorate or become moldy, resulting in malnutrition and infection of the larvae, and thus the larvae die at a high rate. At the same time, if the humidity of the small environment is too high, a large number of adults will be infected with fungi and die, resulting in a significant decrease in the number of adults, which seriously affects the adult lifespan and egg production, and brings great difficulties to the feeding of the next generation. In addition, the accumulation of tubers during the feeding process causes a large number of adults to be crushed to death. Therefore, it is urgent to solve the problems that occur during the feeding and oviposition of sweet potato weevils in order to carry out large-scale successive feeding. Therefore, a method for efficiently feeding sweet potato weevils is proposed. SUMMARY

[0005] The present application aims at solving the problem of large-scale and simple feeding and egg laying of Cylindrocopturus adspersus, and inventing a support body which can be fixed flexibly according to the size of sweet potato, so as to quickly, simply and efficiently ensure the smooth feeding and uniform egg laying of Cylindrocopturus adspersus. By using the method, the laboratory has successfully realized the large-scale rearing of Cylindrocopturus adspersus in the past 5 months, greatly reducing the labor input and improving the survival rate and egg laying amount of Cylindrocopturus adspersus adults.

[0006] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions: a method for efficiently rearing Cylindrocopturus adspersus, comprising the following steps: step one, rearing preparation: preparing 5mm from the ground, arranging 3 layers of clamping rings around the central axis in radial manner, with 3mm distance between layers, 16 clamping rings in each layer, and 16 clamping rings arranged in two rows in front and back to form two circles, 1 red sweet potato can be placed in front and back clamping rings, and the two ends of the red sweet potato extend out of the clamping ring; the clamping ring is made of PE environment-friendly material, is semicircular, has a radius of 5.0cm, and is used for bearing and supporting the two ends of the red sweet potato; a vertical central axis rod is supported by a triangular base, the central axis rod is 13cm long, has a radius of 4.5cm, and is longitudinally located in the middle of the 3 layers of clamping rings, and forms a firm support body with the triangular base; a support ring is used for longitudinally connecting the 3 layers of clamping rings and transversely connecting the clamping rings in the same layer, and the clamping ring is installed thereon; a rearing cage is a transparent 120-mesh square cage with a length of 50.0cm, a width of 50.0cm and a height of 50.0cm; and a rearing box has a length of 43.0cm, a width of 28.5cm and a height of 16.0cm, and the box cover has an opening (length of 18.0cm and width of 15.5cm) which is sealed by a 120-mesh gauze.

[0007] Step two, adult rearing: placing a central axis rod and a support ring on the bottom of the rearing box, installing 3 layers of 24 semitransparent clamping rings on the support ring to form a 3-layer petal-shaped device, placing one fresh sweet potato in each tube, introducing a large number of newly-emerged Cylindrocopturus adspersus adults to the clamping ring, allowing the adults to naturally climb onto the sweet potato to feed and lay eggs, introducing adults of the same age into the clamping ring of the same rearing box, and covering the box cover; after 7 days or so, observing the feeding condition of the sweet potato through the semitransparent gauze and the rearing box body, and replacing the fresh sweet potato in time when the pinhead-sized uniformly distributed holes appear on the surface of the sweet potato, the sweet potato skin begins to dry, and a large number of adults begin to gather on the wall of the rearing box and the box cover.

[0008] Step three, egg collection: sequentially installing a central axis rod, a support ring and a semicircular clamping ring on the triangular base in the rearing box, placing 24 fresh sweet potatoes in 24 pairs of semicircular clamping rings, introducing Cylindrocopturus adspersus under the clamping ring, and covering the rearing box cover; after 7 days or so, the pinhead-sized uniformly distributed pits on the surface of the sweet potato are the traces left by the feeding or egg laying of Cylindrocopturus adspersus adults.

[0009] Step four, larvae feeding: directly bury the petal-shaped device with sweet potato and eggs in three kinds of moist substrates (soil, loose substrate, mixture of soil and loose substrate), the substrate is preferably in contact with the root support, after 15 days, the larvae hatched from the eggs start to eat the sweet potato tissue near the egg pit, as the age increases, the larvae form curved tunnels in the sweet potato, when the larvae pupate and emerge from the sweet potato, the residual sweet potato with almost no pupae is taken out and discarded every 7 days.

[0010] As a preferred technical solution of the present application, the snap ring is made of translucent material, which facilitates the observation of the feeding and oviposition of sweet potato weevils.

[0011] As a preferred technical solution of the present application, the insect rearing cage is a transparent 120-mesh cubic cage, and the opening of the cage cover is sealed with a 120-mesh screen to maintain air circulation.

[0012] As a preferred technical solution of the present application, the rearing site is an insect rearing room, and the rearing conditions are as follows: indoor temperature controlled by air conditioning is 27±1℃, photoperiod is 14:10h, and relative humidity is 70%.

[0013] As a preferred technical solution of the present application, in the adult feeding step, adults of the same age are introduced into the snap ring of the same rearing box to ensure consistency of the rearing environment.

[0014] As a preferred technical solution of the present application, in the adult feeding step, fresh sweet potatoes are replaced after about 7 days according to the feeding situation of sweet potatoes to avoid affecting the growth and reproduction of sweet potato weevils.

[0015] As a preferred technical solution of the present application, in the egg collection step, the pits of pinhead size uniformly distributed on the surface of sweet potatoes after about 7 days can be used as traces left by adult sweet potato weevils to judge whether the adults have fed or laid eggs.

[0016] As a preferred technical solution of the present application, in the larvae feeding step, the three kinds of moist substrates are soil, loose substrate, and mixture of soil and loose substrate, and the substrate is preferably in contact with the root support to avoid dry sweet potatoes, poor ventilation, and difficult conditions for adult emergence.

[0017] As a preferred technical solution of the present application, the triangular base is used to support the vertical central shaft, and the vertical shaft can be stably held up by nesting rings.

[0018] As a preferred technical solution of the present application, the central shaft is 13 cm long and has a radius of 4.5 cm, and is longitudinally located in the middle of the three snap rings, forming a firm support body with the triangular base.

[0019] Compared with the prior art, the present application has the following advantages:

[0020] The method is extremely efficient and simple, has the advantages of simple operation, time saving and recycling, and good feeding effect can be obtained. When replacing the potato pieces, only the soft hair brush is used to gently brush the sweet potato weevil adults, the whole device is moved into a new insect rearing box, and the pre-prepared moist and fluffy substrate is buried. After 10 days, the substrate above the sweet potato is removed and only a thin layer is left, so that the adults can successfully emerge and drill out. The parts on the device can be flexibly installed and detached, the clasp ring is easy to clean and can be reused, and the components are cheap. Therefore, the method not only saves materials and cost, but also greatly improves the efficiency of feeding and replacing sweet potatoes, and is easy to operate.

[0021] The above assembly mode of all parts of the present application can mark the clasp ring, facilitate the further smooth development of subsequent other experiments, and avoid a large amount of manual processing by directly burying, thereby greatly improving the efficiency, avoiding damage to the eggs due to extrusion, and ensuring the hatching of larvae. The feeding device greatly avoids the frequent occurrence of the phenomenon that a large number of adults are crushed to death by the huge sweet potatoes, and improves the survival rate of adults to some extent. The feeding device improves the survival rate of adults while also improving the egg-laying amount of adults.

[0022] In the past feeding process, 10 or so sweet potatoes are directly stacked at the bottom of the insect rearing box and connected to the emerged adults. However, during the feeding and egg-laying process, a large number of adults are often crushed to death by the huge sweet potatoes, which seriously affects the adult lifespan and egg-laying amount, and the frequent replacement of sweet potatoes by humans causes secondary injury to the eggs. The use of the half-moon-shaped clasp ring fundamentally solves this problem. The large clasp ring fixes the two ends of the sweet potato, ensuring that it does not fall off and ensuring that the adults can smoothly climb to the surface of the sweet potato from the bottom of the insect rearing box for feeding and egg-laying. At the same time, each sweet potato is in a suspended state, avoiding the crushing of adults caused by the stacking of several sweet potatoes. In addition, the eggs are evenly laid. These factors enable the sweet potato weevil to efficiently feed and lay eggs, thereby improving the survival rate of adults and the egg-laying amount.

[0023] The mechanical damage caused during the collection of the egg-laying potato pieces is greatly reduced, and the potential damage to the eggs caused by the tweezers and blind handling of the potato pieces is avoided. The half-moon-shaped clasp ring greatly solves the problem of mechanical damage; at the same time, the distance between adjacent clasp rings is very close, and the adults can freely move, feed and lay eggs between different potato pieces, avoiding the damage caused by human transfer, and greatly improving the utilization rate of sweet potatoes. The sweet potato weevil has a strong pseudomortis, and once it encounters a disturbance, it will immediately fall to the ground, reducing the feeding efficiency and egg-laying efficiency of the adults. Therefore, the smooth and free migration of the adults fixed by the half-moon-shaped clasp ring indirectly improves the survival rate and egg-laying amount of the sweet potato weevil adults.

[0024] The present application avoids the frequent occurrence of the phenomenon that the larvae are squeezed to death due to the stacking of sweet potatoes in the feeding box; and avoids the large occurrence of the phenomenon of unbalanced feeding, uneven oviposition or less oviposition.

[0025] According to the strong pseudo-death characteristics of the sweet potato small elephant beetle, the present application uses a low-cost, commercially available snap ring as a unique component for fixing sweet potatoes, so that the bottom of the box does not directly contact the fresh potato pieces, and the potato pieces are not stacked on the bottom of the box. Instead, the adult sweet potato small elephant beetles freely climb onto the surface of fresh potato pieces to feed and lay eggs, thereby avoiding waste and inefficiency, greatly improving the survival rate and oviposition of adult sweet potato small elephant beetles, and improving the utilization rate of potato pieces. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 A method flowchart is provided for the present application;

[0027] Figure 2 An adult feeding management data block diagram is provided for the present application;

[0028] Figure 3 A data parameter block diagram is provided for the present application;

[0029] Figure 4 An egg collection data block diagram is provided for the present application;

[0030] Figure 5 A larva feeding and management data block diagram is provided for the present application;

[0031] Figure 6 A feeding device structure diagram is provided for the present application;

[0032] Figure 7 A feeding device snap ring structure diagram is provided for the present application. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments.

[0034] Therefore, the following detailed description of the embodiments of the application is not intended to limit the scope of the application as claimed, but merely represents some embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the application. It should be noted that the embodiments in the application and the features and technical solutions in the embodiments can be combined with each other without conflict, and similar reference numerals and letters represent similar items in the following drawings, so that once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0035] Embodiment 1: A method for efficiently breeding sweet potato weevils, comprising the following steps: Step one, preparation for feeding: prepare 5mm from the ground, arrange 3 layers of clamps 1 radially around the central axis, 3mm apart between layers, 16 clamps 1 per layer, and arrange the 16 clamps 1 in two rows in front and back to form two circles, 1 red sweet potato can be placed in front and back two clamps 1, and the two ends of the red sweet potato extend out of the clamps 1; the clamps 1 are made of PE environmentally friendly material, are half-moon shaped, have a radius of 5.0cm, and are used to support and hold the two ends of the red sweet potato; a triangular base 2 is used to support a vertical central axis rod 3, the central axis rod 3 is 13cm long and has a radius of 4.5cm, and is longitudinally located in the middle of the 3 layers of clamps 1 and forms a firm support body with the triangular base 2; a support ring 4 is used to longitudinally connect between the 3 layers of clamps 1 and transversely connect between the clamps 1 in the same layer, and the clamps 1 are installed thereon; a rearing cage 5 is a transparent 120-mesh square cage with a length x width x height = 50.0cm x 50.0cm x 50.0cm; and a rearing box 6 is 43.0cm x 28.5cm x 16.0cm in length x width x height, and has an opening in the box cover (18.0cm x 15.5cm in length x width), which is sealed with a 120-mesh gauze 8.

[0036] Step two, adult feeding: place a central axis rod 3 and a support ring 4 on the bottom of the rearing box 6, install 3 layers of 24 translucent clamps 1 on the support ring 4, form a 3-layer petal-shaped device, place one fresh sweet potato in each tube, introduce a large number of newly emerged sweet potato weevil adults to the clamps 1, and let the adults naturally climb onto the sweet potatoes to feed and lay eggs, introduce adults of the same age into the clamps 1 of the same rearing box 6, cover the box cover, and when feeding for about 7 days, observe the feeding condition of the sweet potatoes through the translucent gauze 8 and the rearing box 6 body, replace the fresh sweet potatoes in time when the entire sweet potato surface appears uniformly distributed pinhead-sized holes, the sweet potato skin begins to dry, and a large number of adults begin to gather on the wall and cover of the rearing box 6.

[0037] Step three, egg collection: in the feeding box, install the central axis rod 3, support ring 4 and half-moon-shaped clasp ring 1 on the triangular base 2 in turn, put 24 fresh sweet potatoes into 24 pairs of half-moon-shaped clasp ring 1, connect the sweet potato weevils below the clasp ring 1, cover the insect feeding box 6 cover, and after about 7 days, the needle-sized evenly distributed pits on the surface of the sweet potato are the traces left by the sweet potato weevil adults feeding or laying eggs;

[0038] Step four, larva feeding: directly bury the petal-shaped device and the sweet potato in it with three kinds of moist substrates (soil, fluffy substrate, and mixture of soil and fluffy substrate), and the substrate should be in contact with the root support. After 15 days, the larvae hatched from the eggs start to eat the sweet potato tissue near the egg pit. As the age increases, the larvae form curved tunnels in the sweet potato. After the larvae pupate and emerge from the sweet potato, the substrate is removed after 7 days.

[0039] The clasp ring 1 is made of translucent material, which is convenient for observing the feeding and egg-laying of the sweet potato weevil adults.

[0040] The insect feeding cage 5 is a transparent 120-mesh cube cage, and the opening of the insect feeding box 6 cover is sealed with a 120-mesh screen 8 to maintain air circulation.

[0041] The feeding site is an insect feeding room, and the feeding conditions are as follows: the indoor temperature is controlled by air conditioning at 27±1℃, the light period is 14:10h, and the relative humidity is 70%.

[0042] In the adult feeding step, adults of the same age are introduced into the same clasp ring 1 of the insect feeding box 6 to ensure the consistency of the feeding environment.

[0043] In the adult feeding step, when the feeding is about 7 days, fresh sweet potatoes are replaced in time according to the feeding situation to avoid affecting the growth and reproduction of the sweet potato weevil.

[0044] In the egg collection step, the needle-sized evenly distributed pits on the surface of the sweet potato after about 7 days can be used as the traces left by the sweet potato weevil adults feeding or laying eggs for judgment.

[0045] In the larva feeding step, the three kinds of moist substrates used are soil, fluffy substrate, and mixture of soil and fluffy substrate. The substrate should be in contact with the root support to avoid dryness, poor ventilation, and difficulties in adult emergence.

[0046] The triangular base 2 is used to support the vertical central axis rod 3, which can be stably supported by the nested ring 7. The central axis rod 3 is 13 cm long and has a radius of 4.5 cm. It is longitudinally located in the middle of the three clasp rings 1 and forms a firm support body with the triangular base 2.

[0047] Embodiment 2: A method for efficiently breeding Cylindrocopturus adspersus, 5mm from the ground, 3 layers of snap rings 1 arranged radially around the central axis, 3mm apart between layers, 16 snap rings 1 per layer, 16 snap rings 1 arranged in two rows in front and back, forming two circles, one red sweet potato can be placed in front and back snap rings 1, and the two ends of the red sweet potato extend out of the snap ring 1.

[0048] Snap ring 1: PE environmentally friendly material, half-moon shape, radius 5.0cm, front and back snap rings 1 used to support and hold the two ends of the red sweet potato.

[0049] Triangular base 2: a base for supporting the vertical central axis rod 3, which can be stably held by the nested ring 7.

[0050] Central axis rod 3: 13cm long, radius 4.5cm; longitudinally located in the middle of the 3 layers of snap rings 1, and forms a firm support body with the triangular base 2.

[0051] Support ring 4: a structure that connects longitudinally between the 3 layers of snap rings 1 and transversely between the snap rings 1 in the same layer, on which the snap rings 1 are installed.

[0052] Insect breeding cage 5: l 长 ×w 宽 ×h 高 = 50.0cm x 50.0cm x 50.0cm, transparent 120 mesh cube cage.

[0053] Insect breeding box 6: l 长 ×w 宽 ×h 高 = 43.0cm x 28.5cm x 16.0cm, the box cover has an opening (l 长 ×w 宽 = 18.0cm x 15.5cm) sealed with 120 mesh gauze 8 to maintain air circulation.

[0054] Breeding site: Insect breeding room of Plant Protection and Microbial Economic Crop Pest Control Research Laboratory, Zhejiang Academy of Agricultural Sciences.

[0055] Breeding conditions: indoor temperature controlled by air conditioning at 27±1℃, light cycle 14:10h, relative humidity 70%.

[0056] 1. The method for breeding Cylindrocopturus adspersus is carried out according to the following steps:

[0057] A middle shaft 3 and a support ring 4 are placed at the bottom of the rearing box 6, and 3 layers of 24 translucent clamping rings 1 are installed on the support ring 4, forming a 3-layer petal-shaped device. Each tube contains a fresh sweet potato. A large number of newly emerged sweet potato small elephant beetles are introduced to the clamping ring 1, and the adults are allowed to naturally climb onto the sweet potato to feed and lay eggs. Adults of the same age are introduced to the clamping ring 1 of the same rearing box 6, and the lid is closed. After about 7 days of feeding, the feeding condition of the sweet potato is observed through the translucent mesh 8 and the body of the rearing box 6. When the entire surface of the sweet potato appears evenly distributed pinhead-sized holes, the surface of the sweet potato begins to dry, and a large number of adults begin to gather on the wall of the rearing box 6 and the lid, fresh sweet potatoes are replaced in time Figure 1

[0058] 2. The collection method of sweet potato small elephant beetle eggs is as follows:

[0059] In the rearing box, the middle shaft 3, the support ring 4 and the half-moon-shaped clamping ring 1 are installed in sequence on the triangular base 2. 24 fresh sweet potatoes are placed in 24 pairs of half-moon-shaped clamping rings 1, and sweet potato small elephant beetles are introduced below the clamping ring 1, and the rearing box 6 is closed. After about 7 days, pinhead-sized pits evenly distributed on the surface of the sweet potato can be seen, which are the traces left by the adults of sweet potato small elephant beetles feeding or laying eggs.

[0060] 3. The rearing method of sweet potato small elephant beetle larvae is as follows:

[0061] The petal-shaped device with eggs and the sweet potatoes in it are directly buried with three kinds of moist substrates (soil, fluffy substrate, and mixture of soil and fluffy substrate), and the substrate should be in contact with the roots for support. This can avoid the conditions of dry sweet potatoes, poor ventilation and difficulty in adult emergence. After 15 days, the larvae hatched from the eggs start to feed on the sweet potato tissue near the egg pit. As the age increases, curved tunnels are formed in the sweet potato. After the larvae pupate in the sweet potato and emerge from the sweet potato, the residual sweet potato with almost no pupae in the substrate is removed and discarded every 7 days.

[0062] Test Example 1: Test of sweet potato small elephant beetle rearing and egg production

[0063] Test time: September 1, 2024 to April 30, 2025

[0064] Test site: Insect rearing room of Plant Protection and Microbial Economic Crop Pest Control Research Room of Zhejiang Academy of Agricultural Sciences.

[0065] Test number of insects: 4000.

[0066] Rearing of larvae and replacement of feed: The above method is used.

[0067] ​Test results: Using this method to feed sweet potato weevils, most of the adults survived within the time (3h) and were successfully transferred through the adjacent half-moon shaped clasp 1. The number of adults transferred using the half-moon shaped clasp 1 device accounted for 92.5% to 99.5% of the number of adults introduced, with an average of 96.4% (Table 1). In contrast, as a control, sweet potato weevils collected from the stacked sweet potato tubers had an adult transfer rate of 76.0% to 94.0% of the number of adults introduced, with an average of 86.9% (Table 2); the adult transfer rate after the sweet potato tubers were placed flat was 93.5% to 96.5%, with an average of 95.2%; and the adult transfer rate after the sweet potato tubers were placed vertically against the corners was 80.5% to 94.5%, with an average of 89.9% (2). The mortality rate of sweet potato weevils during feeding using the petal-shaped device was lower, at 1.6% (Table 3); the mortality rate of the controls was higher, at 13.6% (direct stacking), 12.2% (flat placement), and 11.6% (placement against the corners). It can be seen that the new device and its feeding method have a lower mortality rate during the feeding of sweet potato weevils, indirectly increasing the lifespan of the adults and improving the egg-laying capacity of the adults. This method achieves the purpose of large-scale subculture feeding and year-round supply of standard sweet potato weevil sources.

[0068] Table 1, Test results of the adult transfer of sweet potato weevils of the present patent (3h)

[0069]

[0070] Table 2, Test results of the adult transfer of sweet potato weevils of the control method (3h) Number of dead adults (heads) and mortality rate (%) of sweet potato weevils fed by the present patent and the control method Test results

[0071]

[0072] Table 3, Number of dead adults (heads) and mortality rate (%) of sweet potato weevils fed by the present patent and the control method Test results

[0073]

[0074] This method has the following advantages: (1) high efficiency: sweet potato weevils can move freely between sweet potato tubers, reducing the frequent occurrence of false death caused by disturbance, improving feeding efficiency and egg-laying capacity; (2) simple: the device is very simple to make, has a simple structure, is low in cost, and the components can be reused, making it very easy to operate; (3) little disturbance: it not only meets the free living environment of sweet potato weevils, providing a guarantee for the survival rate and egg-laying capacity of sweet potato weevils, but also facilitates direct observation by experimenters. Tests have also shown that the present invention avoids rotting and deterioration caused by stacking of sweet potato tubers, delays and indirectly provides a relatively clean living environment for adults, to some extent, avoids the frequency of bacterial infection, and meets the practical requirements.

[0075] The above examples are only used to illustrate the present application and not to limit the technical solutions described in the present application. Although the present application has been described in detail with reference to the above various embodiments, the present application is not limited to the above specific embodiments. Therefore, any modification or replacement of the present application; and all technical solutions and improvements that do not deviate from the spirit and scope of the application are all included in the scope of the claims of the present application.

Claims

1. A method for efficiently raising sweet potato weevils, characterized in that: The following steps are involved: Step 1. Preparation for feeding: Prepare 3 layers of clamps 5mm above the ground, with a distance of 3mm between layers and 16 clamps in each layer. The 16 clamps are arranged in two circles, front and back. Put a sweet potato in the front and back clamps, with both ends of the sweet potato extending out of the clamps; the clamps are made of PE environmentally friendly material, half-moon shaped, with a radius of 5.0cm, used to carry and hold the two ends of the sweet potato; use a triangular base to support the vertical central axis rod, the central axis rod is 13cm long and 4.5cm in radius, and is located vertically between the 3 layers of clamps. In the middle, it forms a firm support body with the triangular base; the bracket ring is used to connect the three layers of clasps longitudinally and the clasps on the same layer horizontally, and the clasps are installed on it; the insect cage is a transparent 120-mesh cube cage with a length × width × height of 50.0 cm × 50.0 cm × 50.0 cm; the insect box is a length × width × height of 43.0 cm × 28.5 cm × 16.0 cm, and the lid has an opening (length × width = 18.0 cm × 15.5 cm) sealed with a 120-mesh gauze; Step 2: Adult rearing: Place a central axis and a support ring at the bottom of the insect box. Install 3 layers of 24 translucent clasps on the support ring to form a 3-layer petal-shaped device. Place a fresh sweet potato in each tube, and guide a large number of newly emerged sweet potato weevils to the clasps. Let the adults naturally crawl onto the sweet potatoes to feed and lay eggs. Connect adults of the same age to the clasps of the same insect box, close the box lid, and after rearing for 7 days, observe the feeding situation of the sweet potatoes through the translucent gauze and the insect box body. When evenly distributed pinpoint-sized holes appear on the entire surface of the sweet potato, the sweet potato skin begins to dry, and a large number of adults begin to gather on the wall and lid of the insect box, replace the fresh sweet potatoes in time. Step 3: Collecting Eggs: In a breeding box, install the central axis, bracket ring, and half-moon-shaped clamps on the triangular base in sequence. Place 24 fresh sweet potatoes into each of the 24 pairs of half-moon-shaped clamps, insert the sweet potato weevils under the clamps, and close the insect box lid. After 7 days, evenly distributed pinpoint-sized pits will appear on the surface of the sweet potatoes, indicating that they are traces of feeding or egg-laying by the adult sweet potato weevils. Step 4. Larvae rearing: directly bury the petal-shaped device with eggs and the sweet potatoes in it with three kinds of moist substrates (soil, fluffy substrate, and a mixture of soil and fluffy substrate). The substrate should be in contact with the root support. After 15 days, the larvae hatched from the eggs begin to eat the sweet potato tissue near the egg pit. As the age increases, curved tunnels are formed in the sweet potato. After the larvae pupate and emerge from the sweet potato, the remaining sweet potatoes in the substrate that contain almost no pupae are removed and discarded every 7 days.

2. The method for efficiently raising sweet potato weevils according to claim 1, wherein The clamp ring is made of translucent material and is used to observe the feeding and egg-laying conditions of the adult sweet potato weevil.

3. The method for efficiently raising the sweet potato weevil according to claim 2, wherein: The insect raising cage is a transparent 120-mesh cubic cage, and the opening of the insect raising box lid is sealed with a 120-mesh gauze to maintain air circulation.

4. The method for efficiently raising the sweet potato weevil according to claim 3, wherein: The breeding place is an insect breeding room, and the breeding conditions are that the indoor temperature is controlled at 27±1° C. by air conditioning, the photoperiod is 14:10 h, and the relative humidity is 70%.

5. The method for efficiently raising the sweet potato weevil according to claim 4, wherein: In the adult rearing step, adults of the same age are connected to the clasp of the same insect rearing box and the rearing environment is consistent.

6. The method for efficiently raising the sweet potato weevil according to claim 5, wherein: In the adult rearing step, after 7 days of rearing, fresh sweet potatoes should be replaced in time according to the feeding situation of sweet potatoes.

7. The method for efficiently raising the sweet potato weevil according to claim 6, wherein: During the egg collection step, evenly distributed pinpoint-sized pits were observed on the sweet potato surface on the 7th day, which were judged as traces left by the adult sweet potato weevil feeding or laying eggs.

8. The method for efficiently raising the sweet potato weevil according to claim 7, wherein: During the larval rearing step, the three moist substrates used were soil, fluffy substrate, and a mixture of soil and fluffy substrate, with the substrate contacting the root support.

9. The method for efficiently raising the sweet potato weevil according to claim 8, wherein: The triangular base is used to support the vertical central axis rod and firmly hold up the vertical rod through the nested rings.

10. The method for efficiently raising the sweet potato weevil according to claim 9, characterized in that: The central axis is 13 cm long and has a radius of 4.5 cm. It is located longitudinally in the middle of the three layers of clamps and forms a firm support body with the triangular base.

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