SNP (Single Nucleotide Polymorphism) molecular marker related to corn kernel length character and application thereof

CN120796543APending Publication Date: 2025-10-17INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202510925844.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-07
Publication Date
2025-10-17

AI Technical Summary

Technical Problem

The existing technology lacks functional molecular markers that are stably associated with corn kernel length, resulting in long and low efficiency in traditional breeding selection cycles, making it difficult to achieve early and efficient screening of long-grain corn germplasm.

Method used

Based on genome-wide association analysis (GWAS), the SNP site SNP_chr8_74366816 significantly associated with corn kernel length was identified, and the KASP molecular marker was developed. A primer set with FAM and HEX fluorescent linker sequence tags was used to accurately correspond genotype and phenotype, achieving efficient screening at the seedling stage.

Benefits of technology

It has achieved rapid and accurate genetic typing of corn kernel length, breaking through the cycle limitations of traditional breeding, enabling early and efficient screening of long-grain corn germplasm and improving breeding efficiency.

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for regulating and controlling the length of a corn kernel and application of the SNP molecular marker, and belongs to the technical field of molecular genetics. Based on genome-wide association analysis (GWAS), SNP (single nucleotide polymorphism) sites (C / T variation) remarkably related to grain length are identified at chromosome 8 74, 366 and 816bp of a corn B73RefGenv5 genome, and a C allele is a dominant allele. And the nucleotide sequences of the KASP primer group for the locus typing are shown as SEQ ID NO: 1-3. The molecular marker is combined with a KASP technology, high-precision and high-throughput genetic typing detection can be realized, and the molecular marker has the characteristics of good amplification stability and high detection efficiency, is suitable for early prediction of corn kernel length, germplasm resource identification and molecular marker-assisted selection breeding, and has important application value for breeding of new long-grain corn varieties.
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