Embryo heavy ion track acquisition method based on biological stack

Abstract

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of biological materials, and particularly relates to a method for obtaining a heavy ion track of a seed embryo based on a biostack. BACKGROUND

[0002] In space flight, the radiation environment is relatively complex, and the damage to organisms caused by high-energy heavy ions is an important source of biological effects of space radiation. In order to study the influence of heavy ions on organisms, capturing the track of heavy ions is the first step to achieve this goal. A biostack is a kind of device specially used for heavy ion detection. After the biological material is stacked with a solid nuclear track detector, the track of the heavy ion can be captured by the solid nuclear track, and the corresponding organism can be associated.

[0003] In the prior art, the heavy ion hitting experiment of the biostack has the problems of great workload and tedious data collection. For example, the heavy ion data collection of the Shi Shi No. 10 biostack took nearly 2 years. After entering the space station era, the flight time of the biostack is greatly increased, and the carrying capacity of the biostack is greatly improved. The accumulation of space heavy ions on the detection sheet leads to an increase of 30-60 times in the number of heavy ion tracks in the same observation area. Due to the increase in the number of carriers, the workload of data collection is greatly increased. However, due to the repair mechanism of the damage to the organism, the biological material has obvious timeliness after returning to the earth. The length of data collection is directly related to the accuracy of the data.

[0004] Meanwhile, in the problem of determining the relative position and projection contour of the rice seed embryo, the prior art directly replaces the plane projection of the embryo with an ellipse, but the actual size and shape of the seed embryo are not an ellipse with a major axis of 1.5 mm and a minor axis of 1 mm. When the optical microscope is focused to a certain fixed plane, imaging will be blurred at other imaging depths. Moreover, the outline of the biological material is simply described as an ellipse, which will cause a large error in the long-term flight and affect the subsequent research. SUMMARY

[0005] The embodiment of the application provides a method for obtaining a heavy ion track of a seed embryo based on a biostack, which can solve the problem of inaccurate track caused by inaccurate data collection and inaccurate projection of biological material in the prior art.

[0006] In a first aspect, the embodiment of the application provides a method for obtaining a heavy ion track of a seed embryo based on a biostack, comprising: A biological stack obtained after mounting biological samples and detection materials and returning to Earth via space flight is used as an initial biological stack, the initial biological stack is pre-processed to obtain a target biological stack, and six detection piece mounting surfaces of the target biological stack in three dimensions are scanned to obtain an initial scan image of the target biological stack; Marking the initial scan image for the first time based on the center position of the embryo to obtain a target scan image; Laminating the target scan image and the target biological stacked detection sheet to obtain an initial laminated part, and performing a second marking on the non-contact surface of the initial laminated part according to the center position of the embryo to obtain a target laminated part; Scanning the target bonding part with a laser confocal microscope to obtain an image of the embryo, and cutting out the actual projection of the embryo at the center of the embryo in the image based on a preset projection outline; The heavy ion track of the embryo is identified based on the actual projection.

[0007] Optionally, the step of pre-treating the initial bio-stack to obtain the target bio-stack includes: The initial detection sheet in the initial biological stack is etched according to the pretreatment to obtain a target detection sheet, and the target detection sheet is fixed in the initial biological stack at the original position for placing the initial detection sheet to obtain a target biological stack.

[0008] Optionally, the step of marking the initial scan image for the first time based on the center position of the embryo to obtain a target scan image includes: The scanned image is marked for the first time based on the center position of each embryo using image processing software to obtain a target scanned image.

[0009] Optionally, the step of scanning the target bonding component by using a laser confocal microscope to obtain an image of the embryo includes: Scanning the target assembly using a preset laser confocal microscope to obtain a mosaic image of the embryo and sub-viewing field images composed of the mosaic image by computer splicing; Convert the sub-viewing field images according to the attribute data of the spliced ​​image to obtain a target sub-viewing field image; The target sub-field of view images are manually spliced ​​to obtain an ultra-large field of view scanning image of the position of the embryo, and the ultra-large field of view scanning image is used as the imaging image of the embryo.

[0010] Optionally, before the step of cutting out the actual projection of the seed embryo at the center position of the seed embryo in the image based on the preset projection outline, the method further includes: Obtaining activity data and attribute data of the embryo; modeling the embryo based on the activity data and the attribute data by using a 3D modeling software to obtain an embryo model; establishing a planar projection profile of the embryo based on the embryo model, and taking the planar projection profile of the embryo as the preset projection profile.

[0011] Optionally, the step of obtaining the activity data and the attribute data of the embryo comprises: obtaining initial activity data of the embryo based on a tetrazolium staining method, and performing water absorption expansion proportion conversion on the initial activity data to obtain the activity data of the embryo; obtaining initial attribute data of the embryo based on a tetrazolium experiment, the initial attribute data comprising a length of the embryo, a width of the embryo, and a width of a cross-sectional structure of the embryo; determining volume data of the embryo in a dry state according to the initial attribute data and an expansion coefficient of the embryo, and taking the volume data, the length of the embryo, the width of the embryo, and the width of the cross-sectional structure of the embryo together as the attribute data.

[0012] Optionally, the step of modeling the embryo based on the activity data and the attribute data by using a 3D modeling software to obtain an embryo model comprises: obtaining species information of the embryo, and determining average size data of the embryo based on the species information; modeling the embryo based on the activity data, the attribute data, and the average size data by using a 3D modeling software to obtain an embryo model.

[0013] Optionally, the step of establishing a planar projection profile of the embryo based on the embryo model, and taking the planar projection profile of the embryo as the preset projection profile comprises: establishing an initial planar projection profile of the embryo based on the embryo model; arranging the initial planar projection profile according to a preset requirement to obtain the planar projection profile, so that the planar projection profile is profile characterized on a plane.

[0014] Optionally, the step of cropping the actual projection of the embryo based on the preset projection profile at the center position of the embryo in the imaging image comprises: determining direction data of the embryo based on the imaging image; cropping the actual projection of the embryo on the preset projection profile based on the direction data and the center position of the embryo in the imaging image.

[0015] Optionally, the laser confocal microscope is a laser confocal microscope equipped with an electrically driven stage.

[0016] The beneficial effects of the embodiments of the present application compared with the prior art are: The beneficial effects of the embodiments of the present application compared with the prior art are: The beneficial effects of the embodiments of the present application compared with the prior art are: The beneficial effects of the embodiments of the present application compared with the prior art are:

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort on the basis of these drawings.

[0018] Figure 1 is a flowchart of a method for obtaining a heavy ion track of a seed embryo based on a biological stack provided by an embodiment of the present application; Figure 2 is a flowchart of a method for obtaining a heavy ion track of a seed embryo based on a biological stack provided by another embodiment of the present application; Figure 3 is a structural diagram of a biological stack provided by an embodiment of the present application. DETAILED DESCRIPTION

[0019] In the following description, specific details are set forth in order to provide a thorough understanding of the embodiments of the present application. However, persons skilled in the art should understand that the present application can also be implemented in other embodiments without these specific details. In other cases, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present application with unnecessary details.

[0020] It should be understood that the word “comprise” or variations such as “comprises” or “comprising”, when used in this specification and in the accompanying claims, specify the presence of stated features, integers, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof.

[0021] It should also be understood that the term “and / or” when used in this specification and in the claims which follow, unless otherwise stated, means any conceivable combination of one or more of the associated listed items and all possible combinations.

[0022] As used in this specification and in the claims, the term “if’ can be interpreted as meaning “when,” or “once,” or “in response to a determination,” or “in response to a detection,” depending on the context. Similarly, the phrase “if it is determined” or “if [a described condition or event] is detected” can be interpreted as meaning “once it is determined” or “in response to the determination” or “once [the described condition or event] is detected” or “in response to the detection [of the described condition or event],” depending on the context.

[0023] In addition, in the description of the application and in the claims which follow, the terms “first”, “second”, “third”, etc. are used only to distinguish descriptions, and cannot be understood as indicating or implying relative importance.

[0024] Reference in the specification to “one embodiment” or “some embodiments” etc. means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the application. The appearances of the phrases “in one embodiment”, “in some embodiments”, “in other embodiments”, “in additional embodiments”, etc. in various places in the specification are not necessarily all referring to the same embodiment, although they can. The terms “comprise”, “comprises”, “comprising”, “include”, “includes”, “including” and the like are synonymous with “containing” or “comprising”, unless otherwise stated. The terms “comprise”, “comprises”, “comprising”, “include”, “includes”, “including” and the like are synonymous with “containing” or “comprising”, unless otherwise stated.

[0025] Figure 1 A schematic flowchart of a biological stack-based zygote heavy ion track acquisition method provided by the application is shown. The embodiment of the application provides a biological stack-based zygote heavy ion track acquisition method, which comprises: S101, install a biological sample and a detection material, and obtain a biological stack after space flight return to the earth as an initial biological stack, pretreat the initial biological stack to obtain a target biological stack, and scan six detection piece mounting surfaces of the target biological stack in three dimensions to obtain an initial scanning diagram of the target biological stack; In a possible implementation, the step of pre-treating the initial biological stack to obtain a target biological stack comprises: etching the initial detection sheet in the initial biological stack according to the pre-treatment to obtain a target detection sheet, and fixing the target detection sheet in the initial biological stack at an original position where the initial detection sheet is placed to obtain a target biological stack.

[0026] For example, the initial detection sheet in the initial biological stack is etched using a NaOH solution. After etching, the target detection sheet is placed back at the original position of the biological stack, and 3M tape is used to fix the four corners of the target detection sheet (to prevent residue). The target detection sheet is scanned using a Hewlett Packard 4060 scanner, with a scanning resolution of 4800 pixels and saved as a TIFF format picture.

[0027] For example, as shown in Figure 3 the initial biological stack includes a seed 2 and a solid nuclear track material 1.

[0028] S102, first marking the initial scan map based on the center position of the seed to obtain a target scan map; For example, the scan map is first marked based on the center position of each seed by using image processing software, such as PS software, to obtain a target scan map.

[0029] S103, aligning the target scan map with the detection sheet of the target biological stack to obtain an initial alignment piece, and second marking on the non-contact surface of the initial alignment piece according to the center position of the seed to obtain a target alignment piece; For example, the size of the target scan map is adjusted to be the same as that of the detection sheet of the target biological stack, and the target scan map is color printed. The target scan map and the detection sheet of the target biological stack are aligned to obtain an initial alignment piece. A marker pen, such as a marker pen, is used to second mark on the non-contact surface of the initial alignment piece according to the center position of the seed to obtain a target alignment piece.

[0030] S104, scanning the target alignment piece by a laser confocal microscope to obtain an imaging map of the seed, and clipping an actual projection of the seed on the center position of the seed in the imaging map based on a preset projection contour; For example, the system scanning is performed using a VK1000 laser confocal microscope or a metallurgical microscope equipped with a motorized stage. First, the observation is performed using a 4X objective, and the lens is aligned with the projection position of the zygote, and then the observation is performed under a 20X objective. Using the stitching measurement mode of the motorized stage, the scanning range is selected as x x y = 4 x 5 fields, the actual size of the scanning is 2551 x 2375 μm, and it is ensured that the zygote is located at the center of the scanning range. The focal plane is manually set, the scanning depth is set as 1.5 μm, and the scanning is started.

[0031] In a possible implementation, the step of cropping the actual projection of the zygote on the preset projection contour based on the center position of the zygote in the imaging map comprises: determining the direction data of the zygote based on the imaging map; cropping the actual projection of the zygote on the preset projection contour based on the direction data and the center position of the zygote in the imaging map.

[0032] For example, the direction of the zygote (direction data) is determined according to the display content of the imaging map, and the actual projection of the zygote is cropped on the preset projection contour based on the direction of the zygote (direction data) and the center position of the zygote.

[0033] S105, identifying the heavy ion track of the zygote according to the actual projection.

[0034] The biological stack obtained after the biological sample and the detection material are installed and spatially flown back to the earth is taken as an initial biological stack, the target biological stack is obtained by pretreating the initial biological stack, and the initial scanning map of the target biological stack is obtained by scanning the target biological stack; the initial scanning map is marked for the first time based on the center position of the zygote, and the target scanning map is obtained; the target scanning map and the detection sheet of the target biological stack are matched to obtain an initial matching piece, and the second marking is performed on the non-contact surface of the initial matching piece according to the center position of the zygote to obtain a target matching piece; the imaging map of the zygote is obtained by scanning the target matching piece through a preset laser confocal microscope, and the actual projection of the zygote is cropped on the preset projection contour based on the imaging map and the center position of the zygote in the imaging map; and the heavy ion track of the zygote is identified according to the actual projection. Compared with an optical microscope, the preset laser confocal microscope can improve the efficiency of data acquisition, faster data acquisition process, and avoid the timeliness caused by the damage repair mechanism of biological materials. By cropping the actual projection of the zygote on the preset projection contour, the fixed size of the ellipse representing the actual projection of the zygote is avoided, the projection of the zygote is more accurate, and the track determination result is more accurate.

[0035] In a possible implementation, the step of scanning the target fitting part by the laser confocal microscope to obtain the imaging map of the seed embryo includes: scanning the target fitting part by a preset laser confocal microscope to obtain a spliced map of the seed embryo and a sub-field map spliced by a computer to form the spliced map; converting the sub-field map according to attribute data of the spliced map to obtain a target sub-field map; artificially splicing the target sub-field map to obtain an ultra-large field scanning map of a position of the seed embryo, and taking the ultra-large field scanning map as the imaging map of the seed embryo.

[0036] For example, when the computer splices the sub-field maps to form the spliced map of the seed embryo, compression may occur in the splicing process, which may cause the clarity to decrease. Therefore, the sub-field map is converted according to the attribute data (pixel, size, and the like) of the spliced map to obtain a target sub-field map, and the imaging map of the seed embryo is obtained by using the artificial splicing method, so as to ensure the quality of the imaging map and facilitate subsequent track acquisition.

[0037] In a possible implementation, before the step of clipping the actual projection of the seed embryo based on the preset projection contour at the center position of the seed embryo in the imaging map, the method further includes: obtaining activity data and attribute data of the seed embryo; modeling the seed embryo based on the activity data and the attribute data by using a 3D modeling software to obtain a seed embryo model; establishing a planar projection contour of the seed embryo according to the seed embryo model, and taking the planar projection contour of the seed embryo as the preset projection contour.

[0038] For example, the step of obtaining the activity data and the attribute data of the seed embryo includes: obtaining initial activity data of the seed embryo based on a tetrazolium staining method, and performing water absorption and expansion proportion conversion on the initial activity data to obtain the activity data of the seed embryo; obtaining initial attribute data of the seed embryo based on a tetrazolium experiment, the initial attribute data including a length of the seed embryo, a width of the seed embryo, and a width of a cross-sectional structure of the seed embryo; determining volume data of the seed embryo in a dry state according to the initial attribute data and an expansion coefficient of the seed embryo, and taking the volume data, the length of the seed embryo, the width of the seed embryo, and the width of the cross-sectional structure of the seed embryo together as the attribute data. Specifically, the size of the seed active center is determined by using a tetrazolium staining method, and the water absorption expansion ratio of the dry seed is converted. The mechanism of the experiment can refer to the national standard. The width of the seed embryo profile structure, the length of the seed embryo, and the width of the seed embryo are obtained according to the tetrazolium experiment, and the size of the seed embryo when dry is determined according to the determined expansion coefficient.

[0039] In a possible implementation, the step of modeling the seed embryo based on the activity data and the attribute data by using 3D modeling software to obtain a seed embryo model includes: Obtaining the species information of the seed embryo, and determining the average size data of the seed embryo based on the species information; Modeling the seed embryo based on the activity data, the attribute data, and the average size data by using 3D modeling software to obtain a seed embryo model.

[0040] Exemplarily, because the average sizes of seed embryos of different species are quite different, for example, corn seed embryos and rice seed embryos, the species information of the seed embryo needs to be obtained, and then the average size data of the seed embryo is determined. Taking rice seed embryos as an example, 3D reconstruction of the seed embryo is performed by using soildworks, and the built 3D model is given the average size of the rice seed embryo.

[0041] In a possible implementation, the step of establishing the planar projection contour of the seed embryo according to the seed embryo model and taking the planar projection contour of the seed embryo as the preset projection contour includes: Establishing an initial planar projection contour of the seed embryo according to the seed embryo model; Arranging the initial planar projection contour according to a preset requirement to obtain the planar projection contour, so that the planar projection contour is characterized on a plane.

[0042] Exemplarily, the planar projection contour of the rice seed embryo is established, and is processed by PS, so that the size of the specified contour can be circled on the plane.

[0043] In a possible embodiment, in order to better reflect the timeliness of the present scheme, the present scheme is compared with the prior art by means of "man-hour" unit, the data acquisition time of the present method for a single CR-39 detection sheet can be controlled within 15 man-hours, and about 3000 heavy ion tracks are acquired for each detection sheet. Compared with the method used in the biological stack of the Shijian-10 return satellite, the Shijian-10 processed a total of 10 CR-39 sheets, and the total number of data points (single heavy ion track) was less than 1000, and the total time consumed was 606 days, about 60 days for each sheet, a total of 3 people participated (about 1080 man-hours for each sheet, 6 man-hours per day for each person), and the average number of heavy ion tracks for each sheet was about 200. It can be seen that the processing speed of the whole detection sheet is increased by about 70 times, and the number of track detections per man-hour is increased by 37 times.

[0044] In a possible embodiment, as shown in Figure 2 the biological stack-based heavy ion track acquisition method for zygotes described in the present scheme comprises: After the biological stack is returned, the original position of the detection sheet (fixed by silicone rubber, placed loose or fallen off by using adhesive tape) is fixed by using 3M adhesive tape, and then the interface is scanned by using a scanner. Since the actual size of the zygote is smaller than the marker for the zygote, the scanning image of the biological stack is processed at 1:1, and the center position of each zygote in the biological stack is marked, then the pattern is printed, the detection sheet is pasted on the printed pattern, and the center position of the zygote is marked on the non-contact surface by using a marker pen, then a laser confocal microscope is used for scanning, and the scanning area is ensured to be completely positioned on the projection of the zygote, and the center provided by the marker pen is only used for positioning.

[0045] The microscope model used in this method is a Keyence VK1000 laser confocal microscope, equipped with a motorized stage adapted for the microscope. During the measurement, the probe piece with the position mark is placed on the slide and placed in the center of the stage. The laser confocal microscope scans the side of the probe piece that is in contact with the seed. Each probe piece is engraved with the probe piece number, and the engraved side does not contact the seed. The microscope is adjusted through the software to the general measurement mode under the 4x lens. The projection position of the seed embryo to be measured is found in the field of view. Then the microscope magnification is adjusted to 20x and the focal length is adjusted to make the field of view clear. Adjust the light intensity to avoid the field of view being too bright or too dark. The measurement mode is converted to the splicing measurement mode, and the automatic scanning function of the motorized stage is used to generate a scanning field of view of the entire projection site. Set the scanning area to the entire projected area, adjust the scanning depth to 1.5μm, set the scanning plane to the focal plane, and then start scanning. After the scan is complete, two types of data are generated: an image of the entire embryo derived from the stitching measurement function, and images of the sub-fields that form this stitched image. These data are saved as VK files and require multi-file analysis software to open. The actual size of a rice embryo is 1.3×1.1 mm, so generally, a 4×4 field of view is sufficient to capture the surface of the probe.

[0046] After scanning, a surface image of the corresponding position is obtained for subsequent processing. First, the corresponding embryo mosaic pattern is opened using VK software. Due to the compression phenomenon after image mosaicking, the clarity is reduced. After manually superimposing the sub-fields according to the mosaic pattern and the conversion ratio, the embryo model constructed by SoildWorks is used to perform a planar projection outline. The specific projection of the embryo is cut out according to the embryo's direction and center position.

[0047] Those skilled in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of this application.

[0048] In the embodiments provided by the present application, it should be understood that the disclosed apparatus / network device / terminal device and method can be implemented in other manners. For example, the embodiments of the apparatus / network device / terminal device described above are merely schematic; the division of the modules or units is merely logical function division; there can be another division manner in actual implementation; for example, a plurality of units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the displayed or discussed mutual couplings or direct couplings or communication connections between the units can be indirect couplings or communication connections through some interfaces, devices or units, and can be electrical, mechanical or in other forms.

[0049] The units described as separate components can or can not be physically separate, and the components shown as units can or can not be physical units, i.e., can be located in one place, or can be distributed on a plurality of network units. Part or all of the units can be selected according to actual needs to achieve the purpose of the embodiments.

[0050] The above-described embodiments are merely used to illustrate the technical solutions of the present application, but not limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: the technical solutions recorded in the foregoing embodiments can still be modified, or some technical features can be replaced by equivalent replacements; and these modifications or replacements do not make the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application, and should be included in the protection scope of the present application.

Claims

1. A method for obtaining heavy ion tracks of embryos based on bio-stack, characterized in that: include: A biological stack obtained after mounting biological samples and detection materials and returning to Earth via space flight is used as an initial biological stack, the initial biological stack is pre-processed to obtain a target biological stack, and six detection piece mounting surfaces of the target biological stack in three dimensions are scanned to obtain an initial scan image of the target biological stack; Marking the initial scan image for the first time based on the center position of the embryo to obtain a target scan image; Laminating the target scan image and the target biological stacked detection sheet to obtain an initial laminated part, and performing a second marking on the non-contact surface of the initial laminated part according to the center position of the embryo to obtain a target laminated part; Scanning the target bonding part with a laser confocal microscope to obtain an image of the embryo, and cutting out the actual projection of the embryo at the center of the embryo in the image based on a preset projection outline; The heavy ion track of the embryo is identified based on the actual projection.

2. The method for obtaining heavy ion tracks of embryos based on biorepository according to claim 1, wherein: The step of pre-treating the initial biomass to obtain the target biomass comprises: The initial detection sheet in the initial biological stack is etched according to the pretreatment to obtain a target detection sheet, and the target detection sheet is fixed in the initial biological stack at the original position for placing the initial detection sheet to obtain a target biological stack.

3. The method for obtaining heavy ion tracks of embryos based on biorepository according to claim 1, wherein: The step of marking the initial scan image for the first time based on the center position of the embryo to obtain a target scan image includes: The scanned image is marked for the first time based on the center position of each embryo using image processing software to obtain a target scanned image.

4. The method for obtaining heavy ion tracks of embryos based on bio-stack as claimed in claim 1, wherein: The step of scanning the target bonding component by a laser confocal microscope to obtain an image of the embryo includes: Scanning the target assembly part through a preset laser confocal microscope to obtain a mosaic image of the embryo and sub-viewing field images composed of the mosaic image through computer stitching; Convert the sub-viewing field images according to the attribute data of the spliced ​​image to obtain a target sub-viewing field image; The target sub-field of view images are manually spliced ​​to obtain a super-large field of view scanning image of the position of the embryo, and the super-large field of view scanning image is used as the imaging image of the embryo.

5. The method for obtaining heavy ion tracks of embryos based on bio-stack as claimed in claim 1, characterized in that: Before the step of cutting out the actual projection of the seed embryo at the center position of the seed embryo in the image based on the preset projection outline, the following operations need to be completed, including: Obtaining activity data and attribute data of the embryo; Modeling the embryo based on the activity data and the attribute data using 3D modeling software to obtain an embryo model; A plane projection profile of the seed embryo is established according to the seed embryo model, and the plane projection profile of the seed embryo is used as the preset projection profile.

6. The method for obtaining heavy ion tracks of embryos based on biorepository according to claim 5, wherein: The step of obtaining activity data and attribute data of the embryo comprises: Obtaining initial activity data of the seed embryo based on a tetrazolium staining method, and converting the initial activity data into a water absorption swelling ratio to obtain activity data of the seed embryo; Obtaining initial attribute data of the seed embryo based on a tetrazolium experiment, wherein the initial attribute data includes a length of the seed embryo, a width of the seed embryo, and a width of a cross-sectional structure of the seed embryo; The volume data of the seed embryo during drying is determined based on the initial attribute data and the expansion coefficient of the seed embryo, and the volume data, the length of the seed embryo, the width of the seed embryo and the width of the cross-sectional structure of the seed embryo are collectively used as the attribute data.

7. The method for obtaining heavy ion tracks of embryos based on biorepository according to claim 1, wherein: The step of modeling the embryo based on the activity data and the attribute data using 3D modeling software to obtain the embryo model comprises: Acquiring type information of the seed embryos, and determining average size data of the seed embryos based on the type information; The embryo is modeled based on the activity data, the attribute data and the average size data using 3D modeling software to obtain an embryo model.

8. The method for obtaining heavy ion tracks of embryos based on bio-stack as claimed in claim 1, characterized in that: The step of establishing the planar projection profile of the seed embryo according to the seed embryo model and using the planar projection profile of the seed embryo as the preset projection profile comprises: Establishing an initial planar projection profile of the embryo according to the embryo model; The initial plane projection profile is sorted according to preset requirements to obtain the plane projection profile, so that the plane projection profile can be represented on a plane.

9. The method for acquiring heavy ion tracks of embryos based on bio-stack as claimed in claim 1, characterized in that: The step of clipping the actual projection of the seed embryo at the center position of the seed embryo in the image based on the preset projection outline comprises: Determining direction data of the embryo based on the imaging image; The actual projection of the seed embryo is clipped on a preset projection outline based on the direction data and the center position of the seed embryo in the image.

10. The method for obtaining heavy ion tracks of embryos based on bio-stack as claimed in claim 1, characterized in that: The preset laser confocal microscope is a laser confocal microscope equipped with a motorized stage.

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