Multi-effect composition with anti-aging and whitening functions as well as preparation method and application of multi-effect composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a multi-effect composition with anti-aging and whitening functions as well as a preparation method and application of the multi-effect composition. According to the multi-effect composition, the gnaphalium affine extract, the gardenia extract and the fermented rice obtained by fermenting bacillus licheniformis CGMCC1.8805 are specifically mixed, so that the multi-effect composition has the anti-inflammatory and anti-oxidation effects, skin stretching can be promoted and skin elasticity can be achieved while external irritation to skin is resisted, and the multi-effect composition has the effects of resisting inflammation and oxidation. The skin is promoted to absorb nutritional ingredients and the skin barrier function is repaired. Moreover, in the multi-effect composition disclosed by the invention, the used raw materials are all natural sources, green, safe and non-irritant, and the unique smell of the gardenia can endow the product with natural smell, so that the subsequent experience feeling is improved. Therefore, the multi-effect composition provided by the invention can be used for preparing anti-aging and whitening products.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a multi-effect composition with anti-aging and whitening effects, a preparation method and an application thereof. Background Art

[0002] The skin, the largest organ in the human body, not only performs numerous important physiological functions but is also closely linked to our appearance and health. As society progresses, modern people are increasingly prioritizing skin health and recognizing the importance of preventing aging. In addition to sun protection, a healthy diet, and exercise, daily skincare is a key way to slow the progression of skin deterioration.

[0003] The development of multifunctional anti-aging and whitening products is a research hotspot in this field. For example, Chinese patent CN115025000 A discloses a whitening composition with anti-wrinkle and firming functions and its application. It specifically discloses that an amide compound, a reducing coenzyme, and a nutrient are combined to reduce the production of niacin and improve the stability of niacinamide, thereby achieving whitening and anti-aging effects, firming the skin, and repairing the skin barrier. Another example is Chinese patent CN113081910 B, which discloses a repair composition and its application. By combining rhizobium gum, trehalose, tamarind seed polysaccharide, Moringa pterygospermum seed oil, meadowfoam seed oil, tocopheryl acetate, betaine, gardenia fruit extract, sodium mannose phosphate, mannose, niacinamide, and bisabolol, the repair composition combines multiple functions such as moisturizing, whitening, anti-wrinkle, anti-aging, and anti-allergic soothing. It can effectively combat skin problems such as dryness, aging, wrinkles, pigmentation, darkening, and allergies caused by external environmental stimuli, and effectively protect skin health. However, niacinamide is somewhat irritating, and due to the large number of raw materials, unknown reactions may also occur, leading to allergies, which is not conducive to the efficacy of the composition. Summary of the Invention

[0004] The purpose of the present invention is to provide a multi-effect composition with anti-aging and whitening effects, and a preparation method and application thereof, which has the characteristics of safety, good anti-aging and whitening effects.

[0005] The present invention provides a multi-effect composition with anti-aging and whitening effects, which comprises the following substances in parts by mass: 3-5 parts of velvet koji extract, 3-5 parts of gardenia extract and 6-8 parts of rice fermentation product; The rice fermented product is obtained by fermenting Bacillus licheniformis CGMCC1.8805.

[0006] The present invention provides a method for preparing the multi-effect composition described in the above technical solution, comprising the following steps: The sample to be extracted is mixed with a methanol solution having a volume concentration of 45%-55%, and ultrasonic extraction and reduced pressure concentration are performed in sequence to prepare the velvet koji extract and the gardenia extract, respectively; mixing rice with water and soaking the mixture to obtain a mixture, adding Bacillus licheniformis CGMCC1.8805 to the mixture, and then performing anaerobic fermentation, filtering and freeze-drying in sequence to obtain a rice fermentation product; The velvet koji extract, gardenia jasminoides extract and rice fermentation product are mixed to obtain a multi-effect composition.

[0007] Preferably, when preparing the extract of Edelweiss, the sample to be extracted includes: flowers of Edelweiss.

[0008] Preferably, during ultrasonic extraction, the mass volume ratio of the sample to be extracted to the methanol solution is 1 g: 12-16 mL.

[0009] Preferably, the parameters of the ultrasonic extraction include: temperature of 38-42°C, time of 55-65 min, and power of 580-650w.

[0010] Preferably, when mixed and soaked, the mass volume ratio of rice to water is 1g:3-5mL; The parameters of the mixed soaking include: temperature of 25-28° C. and time of 15-18 hours.

[0011] Preferably, during anaerobic fermentation, the mass ratio of the mixture to Bacillus licheniformis CGMCC1.8805 is 100:1.5-3; The effective viable count of the Bacillus licheniformis CGMCC1.8805 is 1×10 8 -2×10 8 cfu / g.

[0012] Preferably, the parameters of the anaerobic fermentation include: a time of 3-4 days and a temperature of 45-50°C.

[0013] The present invention provides the use of the multi-effect composition described in the above technical solution in the preparation of anti-aging and / or whitening products.

[0014] The present invention provides a product with anti-aging and whitening effects, comprising: the multi-effect composition described in the above technical solution.

[0015] Beneficial effects: The present invention provides a multi-effect composition with anti-aging and whitening effects. The multi-effect composition comprises the following substances, calculated by weight: 3-5 parts of velvet koji extract, 3-5 parts of gardenia extract and 6-8 parts of rice fermentation product; the rice fermentation product is obtained by fermenting Bacillus licheniformis CGMCC1.8805.

[0016] First, in the multi-effect composition described in the present invention, the added velvet koji extract has anti-inflammatory and antioxidant effects. While resisting external irritation to the skin, it can also promote skin stretching, thereby giving the skin elasticity; the gardenia extract is beneficial for promoting the skin to absorb nutrients; and the rice fermentation obtained by fermenting Bacillus licheniformis CGMCC1.8805 can replenish skin moisture and repair the skin barrier function. Therefore, after the above three substances are specifically compounded, the multi-effect composition has anti-aging and whitening effects. Secondly, in the multi-effect composition described in the present invention, the raw materials used are all of natural origin, green, safe and non-irritating, and the unique smell of gardenia can give the product a natural flavor, enhancing the subsequent experience.

[0017] Therefore, the multi-effect composition provided by the present invention can be used to prepare products with anti-aging and whitening effects, and has the characteristics of being green and safe. DETAILED DESCRIPTION

[0018] In order to further illustrate the present invention, the solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be understood as limiting the scope of protection of the present invention.

[0019] Example 1 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) Preparation of tinder koji grass ( GNAPHALIUM LIONTOPODIUM ) extract Clean and dry the flowers of the tinderella var. truncatula and set aside for later use, and record them as the tinderella var. truncatula samples to be collected; The sample of the velvet koji grass to be extracted was mixed with a methanol solution having a volume concentration of 55% at a mass volume ratio of 1 g:12 mL, and ultrasonically extracted at 42°C and 650W for 55 minutes to obtain an ultrasonic extract; the ultrasonic extract was concentrated under reduced pressure to ensure removal of excess solvent, thereby obtaining the velvet koji grass extract; (2) Preparation of Gardenia jasminoides ( Gardenia jasminoides J. Ellis ) extract Wash the gardenias, dry them and set them aside for later use, recording them as the gardenia samples to be collected; The gardenia flower sample to be extracted was mixed with a 50% methanol solution at a mass volume ratio of 1 g:15 mL, and ultrasonically extracted at 42°C and 650W for 60 minutes to obtain an ultrasonic extract; the ultrasonic extract was concentrated under reduced pressure to ensure the removal of excess solvent, thereby obtaining a gardenia flower extract; (3) Preparation of rice fermentation The effective viable count is 1×10 8 cfu / g of Bacillus licheniformis ( Bacillus licheniformis ) CGMCC1.8805, spare; Select healthy and plump rice and grind it to obtain rice flour with a particle size of ≤0.1cm for later use; Rice flour and water were mixed and soaked at 28°C in a mass-volume ratio of 1 g:5 mL for 15 hours to obtain an immersion material; the immersion material and Bacillus licheniformis CGMCC1.8805 were added to the immersion material in a mass ratio of 100:3, and anaerobically fermented at 50°C for 4 days. The fermentation material was filtered through 5 layers of gauze to obtain a filtrate, and the filtrate was freeze-dried to obtain a rice fermentation material; (4) By weight, 5 parts of velvet koji extract, 5 parts of gardenia extract and 6 parts of rice fermentation product were mixed evenly to obtain a multi-effect composition.

[0020] Example 2 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) The process is the same as step (1) in Example 1; (2) Preparation of Gardenia jasminoides extract Wash the gardenias, dry them and set them aside for later use, recording them as the gardenia samples to be collected; The gardenia flower sample to be extracted was mixed with a methanol solution having a volume concentration of 55% at a mass volume ratio of 1 g:12 mL, and ultrasonically extracted at 42°C and 650W for 55 minutes to obtain an ultrasonic extract; the ultrasonic extract was concentrated under reduced pressure to ensure removal of excess solvent, thereby obtaining a gardenia flower extract; (3) The process is the same as step (3) in Example 1; (4) By weight, 3 parts of velvet koji extract, 4 parts of gardenia extract and 6 parts of rice fermentation product were mixed evenly to obtain a multi-effect composition.

[0021] Comparative Example 1 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) Preparation of velvet koji extract The roots of the tinder grass were cleaned, dried and set aside for later use, and recorded as the tinder grass sample to be collected; The sample of the velvet koji grass to be extracted was mixed with a methanol solution having a volume concentration of 55% at a mass volume ratio of 1 g:12 mL, and ultrasonically extracted at 42°C and 650W for 55 minutes to obtain an ultrasonic extract; the ultrasonic extract was concentrated under reduced pressure to ensure removal of excess solvent, thereby obtaining the velvet koji grass extract; (2) The process is the same as step (2) in Example 1; (3) The process is the same as step (3) in Example 1; (4) The process is the same as step (4) in Example 1.

[0022] Comparative Example 2 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) The process is the same as step (1) in Example 1; (2) Preparation of Gardenia Fruit Extract Wash the gardenia fruit, dry it and set aside, and record it as the gardenia fruit sample to be collected; The gardenia fruit sample to be extracted was mixed with a 50% methanol solution at a mass volume ratio of 1 g:15 mL, and ultrasonically extracted at 42°C and 650W for 60 minutes to obtain an ultrasonic extract; the ultrasonic extract was concentrated under reduced pressure to ensure the removal of excess solvent, thereby obtaining a gardenia fruit extract; (3) The process is the same as step (3) in Example 1; (4) The process is the same as step (4) in Example 1.

[0023] Comparative Example 3 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) The process is the same as step (1) in Example 1; (2) The process is the same as step (2) in Example 1; (3) Preparation of rice fermentation The effective viable count is 1×10 8 cfu / g of Lactobacillus plantarum ( Lactobacillus plantarum ),spare; Select healthy and plump rice and grind it to obtain rice flour with a particle size of ≤0.1cm for later use; Rice flour and water were mixed and soaked at 28° C. in a mass-to-volume ratio of 1 g:5 mL for 15 hours to obtain an immersion product; the immersion product and Lactobacillus plantarum were added to the immersion product in a mass ratio of 100:3, and anaerobically fermented at 50° C. for 4 days. The fermentation product was filtered through 5 layers of gauze to obtain a filtrate, and the filtrate was freeze-dried to obtain a rice fermentation product; (4) The process is the same as step (4) in Example 1.

[0024] Comparative Example 4 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) The process is the same as step (1) in Example 1; (2) The process is the same as step (2) in Example 1; (3) Preparation of rice fermentation The effective viable count is 1×10 8 cfu / g of Bacillus licheniformis ( Bacillus licheniformis ) CGMCC1.8805, spare; Select healthy and plump rice and grind it to obtain rice flour with a particle size of ≤0.1cm for later use; Rice flour, water and Bacillus licheniformis CGMCC1.8805 were mixed uniformly in a mass ratio of 1:10:0.03, and anaerobically fermented at 50°C for 4 days. The fermentation product was filtered through 5 layers of gauze to obtain a filtrate, and the filtrate was freeze-dried to obtain a rice fermentation product. (4) The process is the same as step (4) in Example 1.

[0025] Comparative Example 5 The preparation of a multi-effect composition with anti-aging and whitening properties comprises the following steps: (1) The process is the same as step (1) in Example 1; (2) The process is the same as step (2) in Example 1; (3) The process is the same as step (3) in Example 1; (4) By weight, 3 parts of velvet koji extract, 3 parts of gardenia extract and 10 parts of rice fermentation product were mixed evenly to obtain a multi-effect composition.

[0026] Test 1DPPH-free radical scavenging assay The higher the DPPH-free radical scavenging rate, the better the anti-wrinkle effect of the multi-effect composition. The multi-effect compositions prepared in Examples 1-2 and Comparative Examples 1-5 were preliminarily screened for their anti-wrinkle effects. The specific process is as follows: Using water as a solvent, the multi-functional compositions prepared in Examples 1-2 and Comparative Examples 1-5 were dissolved to obtain sample solutions with a final concentration of 1 mg / mL. Sample group: sample solution 100 μL + DPPH solution 100 μL; Blank group: 100 μL sample solution + 100 μL water; Control group: 100 μL DPPH solution + 100 μL water; Each treatment group was repeated three times in parallel, and the DPPH clearance rate was calculated; where DPPH clearance rate = (1-(A1-A0) / A2) × 100%; Among them, A1 is the absorbance value of the sample group, A0 is the absorbance value of the blank group, and A2 is the absorbance value of the control group. The results are shown in Table 1; Table 1 Free radical scavenging rate of the multi-effect composition in different treatments

[0027] From the data in Table 1, it can be seen that the DPPH scavenging rates in Example 1, Example 2, Comparative Example 2 and Comparative Example 5 are relatively high. Therefore, the multi-effect composition prepared in the present invention has a good antioxidant effect.

[0028] Test 2 Tyrosinase activity inhibition test The higher the tyrosinase activity inhibition, the better the whitening effect of the multi-effect composition. The multi-effect compositions in Example 1, Example 2, Comparative Example 2 and Comparative Example 5 were subjected to whitening verification, and the process was as follows: Using water as a solvent, the multi-functional compositions of Examples 1-2, Comparative Example 2, and Comparative Example 5 were dissolved to obtain test solutions with a final concentration of 1 mg / mL. B16 cells were seeded in 96-well plates at a density of 1×10 4 Cells were cultured in a conventional manner to allow the cells to adhere to the wall, and the following treatment groups were set up, with 3 parallel replicates for each treatment group: Blank group: 100 μL of regular culture medium (the culture medium does not contain the test substance) was added to each well; Positive group: 100 μL kojic acid was added to each well; Sample group: add 100 μL of each test solution to each well (for example, in Example 1, add 100 μL of the test solution prepared in Example 1 to each well); After 48 hours of routine culture, the tyrosinase activity inhibition rate was calculated according to the instructions of the tyrosinase activity detection kit. The results are shown in Table 2: Table 2 Tyrosinase activity inhibition rate in different treatments

[0029] From the data in Table 2, it can be seen that compared with Comparative Example 5, the multi-effect compositions prepared in Example 1, Example 2 and Comparative Example 2 have better tyrosinase activity inhibition rates and better whitening activity.

[0030] Test 3 Safety test New Zealand rabbits were used as test subjects. The left and right sides of the rabbits' backs were hair-removed, and the skin was kept intact (the hair-removed areas were 1.5 cm × 1.5 cm respectively). 1 mL of each test solution from Example 1, Example 2, and Comparative Example 2 (final concentration of the test solution was 1 mg / mL) was evenly applied to the hairless area on the left side of the rabbit's back. The area was then patched and secured with non-irritating adhesive tape and a bandage. Simultaneously, a patch was applied to the hairy area on the right side of the rabbit's back as a control. Three replicates were performed for each treatment. The non-irritating tape and bandage were removed 4 hours after application, and the skin reaction at the test site was observed 24 and 48 hours after application of the test solution. The average score for each treatment was calculated according to the following criteria to determine the skin irritation intensity. The results are shown in Table 3.

[0031] The scoring criteria for skin reactions (including erythema and edema) are as follows, with a maximum score of 8: Erythema reaction: no erythema (0 points), mild erythema (1 point), obvious erythema (2 points), severe erythema, i.e., purple-red spots (3 points), purple-red spots with mild eschar (4 points); Edema reaction: no edema (0 points), mild edema (1 point), mild edema with contour (2 points), moderate edema with skin swelling (3 points), and severe edema with large area and skin swelling (4 points).

[0032] The skin irritation intensity classification standards are as follows: Non-irritating (0 to <0.5 points), mildly irritating (0.5 to <2.0 points), moderately irritating (2.0 to <6.0 points), and strongly irritating (6.0 to <8.0 points).

[0033] Table 3 Scoring results in different treatments

[0034] From the data in Table 3, it can be seen that the multi-effect compositions prepared in Example 1, Example 2 and Comparative Example 2 are all safe and reliable, and have no irritation to the skin.

[0035] Test 4 In vitro collagen production assay The multifunctional compositions prepared in Example 1, Example 2, and Comparative Example 2 were dissolved in human fibroblast culture medium to obtain test solutions with a final concentration of 20 μM; Human fibroblasts were cultured in human fibroblast culture medium at a rate of 2 × 10 5 After confirming cell adhesion, the following treatment groups were set up, with 3 replicates per treatment group: Blank group: 2 mL of human fibroblast culture medium was added to each well (the culture medium did not contain sample solution); Sample group: add 2 mL of each test solution to each well; After 24 hours of conventional culture, the growth of type 1 collagen was measured using a collagen type I C-peptide enzyme immunoassay kit, and the growth rate of collagen in each treatment was statistically analyzed. The results are shown in Table 4.

[0036] Table 4 Growth rate of type 1 collagen in different treatments

[0037] As shown in Table 4, the growth rate of collagen in Comparative Example 2 was only 80% relative to the blank group, while the growth rate in the Examples was as high as 142%-150%. The growth rates of collagen in the Examples were 70% and 62% higher than those in the Comparative Examples, respectively. This shows that the multi-effect composition prepared by the present invention has a better in vitro anti-aging effect.

[0038] In summary, the present invention specifically mixes the extract of velvet koji, gardenia extract and the rice fermentation obtained by fermentation with Bacillus licheniformis CGMCC1.8805, so that the multi-effect composition has anti-inflammatory and antioxidant effects. While resisting external irritation to the skin, it can also promote skin stretching, give skin elasticity, promote skin absorption of nutrients, and repair skin barrier function. In addition, in the multi-effect composition of the present invention, the raw materials used are all of natural origin, green, safe and non-irritating, and the unique smell of gardenia can give the product a natural flavor and enhance the subsequent experience. Therefore, the multi-effect composition provided by the present invention can be used to prepare products with anti-aging and whitening properties.

[0039] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A multi-effect composition with anti-aging and whitening properties, characterized in that: The multi-effect composition comprises the following substances in parts by mass: 3-5 parts of velvet koji extract, 3-5 parts of gardenia extract and 6-8 parts of rice fermentation; The rice fermentation product is obtained by fermenting Bacillus licheniformis CGMCC1.8805.

2. The method for preparing the multi-effect composition according to claim 1, characterized in that: The steps include: The sample to be extracted is mixed with a methanol solution having a volume concentration of 45%-55%, and ultrasonic extraction and reduced pressure concentration are performed in sequence to prepare the velvet koji extract and the gardenia extract, respectively; mixing rice with water and soaking the mixture to obtain a mixture, adding Bacillus licheniformis CGMCC1.8805 to the mixture, and then performing anaerobic fermentation, filtering and freeze-drying in sequence to obtain a rice fermentation product; The velvet koji extract, gardenia jasminoides extract and rice fermentation product are mixed to obtain a multi-effect composition.

3. The preparation method according to claim 2, characterized in that When preparing the extract of Edelweiss, the sample to be extracted includes: flowers of Edelweiss.

4. The preparation method according to claim 2, characterized in that During ultrasonic extraction, the mass volume ratio of the sample to be extracted to the methanol solution is 1 g: 12-16 mL.

5. The preparation method according to claim 2 or 4, characterized in that The parameters of the ultrasonic extraction include: temperature of 38-42° C., time of 55-65 min, and power of 580-650 W.

6. The preparation method according to claim 2, characterized in that When mixed and soaked, the mass volume ratio of rice to water is 1g:3-5mL; The parameters of the mixed soaking include: temperature of 25-28° C. and time of 15-18 hours.

7. The preparation method according to claim 2, characterized in that During anaerobic fermentation, the mass ratio of the mixture to Bacillus licheniformis CGMCC1.8805 is 100:1.5-3; The effective viable count of the Bacillus licheniformis CGMCC1.8805 is 1×10 8 -2×10 8 cfu / g.

8. The preparation method according to claim 2 or 7, characterized in that The parameters of the anaerobic fermentation include: a time of 3-4 days and a temperature of 45-50°C.

9. Use of the multi-effect composition according to claim 1 in the preparation of anti-aging and / or whitening products.

10. A product with anti-aging and whitening effects, characterized in that: The product comprises: the multi-effect composition according to claim 1.

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