Method for rapidly propagating solieria seedlings

Abstract

The invention discloses a method for rapidly propagating solieria seedlings, and relates to the technical field of solieria cultivation. The invention provides a method suitable for rapid propagation of solieria seedlings in a land-based artificial regulation environment. The whole set of method combines a first-stage propagation process and a second-stage propagation process. When primary propagation is carried out, immature quartophyte or male and female gametophyte fronds are used as propagation mother branches, a plurality of independent offspring seeds are obtained after cleaning, cutting and seedling separation treatment, then the cut independent offspring seeds are inoculated into a culture container of an algae culture chamber for suspension aeration culture, and the offspring seeds are promoted to recover, germinate and grow; during second-stage propagation, the solieria seedlings subjected to first-stage propagation are inoculated into a floating frame with holes and placed in a circulating water seedling propagation pool for rapid propagation. The whole propagation process is completed in a land-based artificial regulation environment, and the method has the advantages of being high in propagation efficiency, short in cultivation period, long in suitable cultivation period, high in seedling survival rate and budding rate and the like.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of red seaweed cultivation, and particularly relates to a method for rapidly propagating red seaweed seedlings. BACKGROUND

[0002] Solieriaceae seaweed representative species include Betaphycus, Kappaphycus, Eucheuma and Solieria. Solieriaceae seaweed has very important economic value and ecological value, can be directly eaten and used as medicine, can be used as a raw material for extracting carrageenan, can be used as high-quality feed for aquatic animals, and can also play a role in purifying water quality in marine ecological restoration and tail water treatment. At present, the research on Solieriaceae seaweed at home and abroad mainly focuses on the Eucheumatoideae (Betaphycus, Kappaphycus and Eucheuma), and Eucheuma has gradually become one of the largest cultivated seaweeds in the world, second only to Japanese kelp. Although the seaweed seedling breeding and cultivation technology of Eucheumatoideae seaweed abroad is relatively mature, the overall yield of Eucheuma cultivated in China is very small compared with the global yield. Therefore, China urgently needs to innovate and develop other Solieriaceae seaweed advantage species resources, establish stable and effective seedling cultivation technology and cultivation technology, so as to truly promote the development of China's Solieriaceae seaweed cultivation industry.

[0003] At present, there are only related researches on classification and identification, nutritional analysis, carrageenan extraction and separation of active ingredients of Solieria seaweed at home and abroad, and the research on Solieria seaweed seedling breeding and cultivation technology is still in the blank stage. The Solieria seaweed reported in China includes two species, Solieria pacifica and Solieria tenuis, which have great development value. However, the seedling propagation technology of Solieria seaweed is still in the development stage, so it is necessary to develop a method for rapidly propagating Chinese native Solieria seaweed seedlings. SUMMARY

[0004] The main purpose of the present application is to provide a method for rapidly propagating Solieria seaweed seedlings, which aims to solve the problem of lack of effective means for Solieria seaweed seedling breeding.

[0005] To achieve the above purpose, the present application provides a method for rapidly propagating Solieria seaweed seedlings, which comprises the following steps:

[0006] S1, providing immature tetrasporophytes or gametophytes of Solieria seaweed, and cleaning the algae to remove surface miscellaneous algae to obtain propagation mother bodies;

[0007] S2, cutting and dividing the propagation mother bodies to obtain independent seedlings;

[0008] S3, inoculating the independent seedling into a culture container containing culture solution, and performing suspension aeration culture until the length of the independent seedling reaches 3-5 cm, to obtain first-level propagated red seaweed seedling;

[0009] S4, inoculating the first-level propagated red seaweed seedling into a floating frame with holes, and fixing the upper end of the first-level propagated red seaweed seedling with a seedling fixing net, and then putting the inoculated floating frame with holes into a circulating water seedling propagation tank for rapid propagation until the length of the seedling exceeds the seedling fixing net by 5-8 cm, to obtain second-level propagated red seaweed seedling.

[0010] In an embodiment, in step S1,

[0011] The red seaweed includes any one of Pacific red seaweed and Delisea pulchra; and / or,

[0012] The cleaning step includes: brushing the surface layer of the algal body with a brush, and then soaking in fresh water for 10-20 s to remove surface layer miscellaneous algae, and then cleaning with sterilized seawater for 2-3 times.

[0013] In an embodiment, in step S2, the length of the independent seedling is 1-2 cm.

[0014] In an embodiment, in step S3,

[0015] The conditions of the suspension aeration culture include: water temperature 25-33℃, salinity 25-30, light intensity 30-60 μmol*m -2 *s -1 ; and / or,

[0016] The culture solution includes culture solution obtained by mixing F / 2 culture medium and filtered seawater at a volume ratio of (0.5-1.5):1000, and the amount of the culture solution is calculated as 5 L of culture solution per 100-250 g of independent seedling; and / or,

[0017] In the process of the suspension aeration culture, the pumped gas is gas filtered through a 50 mm air filter membrane.

[0018] In an embodiment, in step S4, the mesh diameter of the seedling fixing net is 1-1.5 cm.

[0019] In an embodiment, in step S4, the coverage density of the first-level propagated red seaweed seedling in the floating frame with holes is 1-1.5 kg / m 2 .

[0020] In an embodiment, in step S4, the conditions of the rapid propagation include: water temperature 20-33℃, salinity 25-30, light intensity 60-120 μmol*m-2 *s -1 .

[0021] In one embodiment, the recirculating water seedling propagation pond is a land-based water pond, and the water entering and exiting the recirculating water seedling propagation pond is sand-filtered seawater.

[0022] In one embodiment, prior to step S4, the following steps are included:

[0023] The circulating water seedling propagation pond was disinfected.

[0024] This invention provides a method for the rapid propagation of *Gnaphalium affine* seedlings under terrestrial artificial controlled environments. This method leverages the asexual reproduction and rapid growth characteristics of *Gnaphalium affine*, comprising primary and secondary propagation processes. In primary propagation, immature tetrasporophytes or gametophytes are used as propagation mother branches. After cleaning and cutting, multiple independent seedlings are obtained. These individual seedlings are then inoculated into culture containers containing nutrient solution for suspension and aeration culture, promoting wound healing, budding, and growth. In secondary propagation, the primary propagated *Gnaphalium affine* seedlings are inoculated into perforated floating frames and placed in a recirculating water seedling propagation tank. The larger space and environment of secondary propagation facilitate rapid seedling growth and achieve rapid propagation. The propagation method provided by this invention can obtain a large number of high-quality *Gnaphalium affine* seedlings in a short period of time. This not only solves the seedling source problem to a certain extent, but also produces seedlings with stable growth, a survival rate and germination rate of 89%–100%, and good repeatability. These seedlings can be reused for successive propagation, significantly improving seedling utilization and facilitating a rapid and stable increase in seedling scale. Furthermore, the propagation method provided by this invention has low equipment requirements, low construction and operating costs, high controllability, and good operability and environmental adaptability. Propagation under artificially controlled conditions allows for year-round seedling propagation and preservation, effectively solving the problems of seedling preservation, propagation, and cultivation difficulties in low winter temperatures, reducing seasonal limitations. In addition, the *Gnaphalium affine* seedlings propagated by this invention can be directly applied to land-based industrial propagation or cultivation. This avoids the risks of uncontrollable factors in marine aquaculture, and the long suitable cultivation period for land-based industrialization (from March to November) extends the suitable cultivation period, thus demonstrating good prospects for widespread application. Attached Figure Description

[0025] In order to make the technical solutions in the embodiments of the present application or the prior art clearer, the accompanying drawings needed in the embodiments or prior art description will be briefly introduced. Obviously, the accompanying drawings in the following description only need to be some embodiments of the present application, and for ordinary skilled in the art, other drawings can be obtained without creative labor based on the drawings shown.

[0026] Figure 1 Figure for the germination and growth results of the red seaweed seedlings of different seedling lengths in Example 1 of the present application after being cultivated for 10 days;

[0027] Figure 2 Figure for the germination and growth results of the red seaweed seedlings under different temperature conditions in Example 1 of the present application after being cultivated for 10 days;

[0028] Figure 3 Figure for the germination and growth results of the red seaweed seedlings under different light conditions in Example 1 of the present application after being cultivated for 10 days;

[0029] Figure 4 Figure for the germination and growth results of the red seaweed seedlings under different salinity conditions in Example 1 of the present application after being cultivated for 10 days;

[0030] Figure 5 Figure for the red seaweed seedlings of primary expansion in Example 1 of the present application;

[0031] Figure 6 Figure for the inoculation in Example 2 of the present application;

[0032] Figure 7 Figure for the red seaweed seedlings of secondary expansion in Example 2 of the present application, wherein, Figure 7 (A) is a figure for the germination results of the red seaweed after being expanded for 10-20 days, Figure 7 (B) is a figure for the red seaweed after being expanded for 20-30 days.

[0033] The implementation, functional features and advantages of the present application will be further described with reference to the embodiments and the accompanying drawings. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the present application.

[0035] It should be noted that if the embodiments of the present application involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement condition, etc. between the components in a certain posture, and if the certain posture changes, the directional indications will also change accordingly.

[0036] In addition, if the embodiments of the present application involve descriptions such as "first", "second", etc., the descriptions of "first", "second", etc. are only for description purposes and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second" can explicitly or implicitly include at least one of the features. In addition, "and / or" or "and / or" appearing throughout the text means that the three parallel schemes are included, for example, "A and / or B" includes A scheme, or B scheme, or A and B are satisfied simultaneously. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of the ordinary skilled in the art, and when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist and is not within the protection scope required by the present application.

[0037] At present, there are only related researches on classification identification, nutrition analysis, carrageenan preparation and separation and extraction of active ingredients of red seaweed, and the seedling breeding and culture technology of red seaweed is in the blank stage. The red seaweed reported in China includes two species of Pacific red seaweed and fine weak red seaweed, which has great development value. However, the seedling propagation technology is in the stage of non-development, therefore, it is necessary to develop a method for rapidly propagating Chinese native red seaweed seedlings.

[0038] Most seaweeds are difficult to propagate seedlings asexually from the perspective of industrialization, and mature seaweed industries such as kelp and seaweed are propagated by sexual reproduction. Other seaweeds in the prior art also use asexual reproduction, but most of them need to rely on the sea area, and there are growth limitations in land-based propagation, for example, the Gracilaria can be asexually propagated by spore body or gametophyte in the sea area or pond, but there is a growth limitation in land-based propagation; for example, the sea grape is asexually cultured by creeping stems or upright branches, and relies on land-based cement pools or sea area cultivation, and there is a survival difficulty problem in the algae cultivation room.

[0039] Therefore, the present application provides a method for rapidly propagating red seaweed seedlings, comprising the following steps:

[0040] S1, providing immature tetrasporic or gametophytic algae of red seaweed, and cleaning the algae to remove surface miscellaneous algae to obtain propagation mother bodies;

[0041] S2, cutting and propagating the propagation mother bodies to obtain independent seedlings.

[0042] S3, inoculating the independent seedlings into a culture container containing culture solution to perform suspension aeration culture until the length of the independent seedlings reaches 3-5 cm, to obtain first-level proliferated red seaweed seedlings;

[0043] S4, inoculating the first-level proliferated red seaweed seedlings into a floating frame with holes, and fixing the upper end of the first-level proliferated red seaweed seedlings with a seedling fixing net, and then putting the inoculated floating frame with holes into a circulating water seedling proliferation pond to perform rapid proliferation until the length of the seedlings exceeds the seedling fixing net by 5-8 cm, to obtain second-level proliferated red seaweed seedlings.

[0044] The inventors found that mature tetrasporophytes or female gametophytes with cystocarps as algae seeds will cause the algae to be white and lost due to the release of tetraspores or carpospores, and cannot achieve the purpose of asexual rapid proliferation, while the use of immature tetrasporophytes or female and male gametophytes can maintain the asexual reproduction and normal growth of algae under the set culture conditions, and achieve the purpose of proliferating seedlings. Among them, the "mature tetrasporophytes" refers to individuals that have developed completely and can release tetraspores; the "immature tetrasporophytes" refers to tetrasporophytes that have not developed to the stage of releasing tetraspores; the "female and male gametophytes with cystocarps" refers to mature gametophytes that have completed fertilization and developed cystocarps on female gametophytes; and the "immature female and male gametophytes" refers to gametophytes that have not developed to the stage of producing gametes for sexual reproduction.

[0045] Based on the above background, the technical scheme of the present application provides a method for rapidly proliferating red seaweed seedlings in a land-based artificial controlled environment, which combines the characteristics of asexual reproduction and fast growth of red seaweed and includes first-level proliferation and second-level proliferation processes. During the first-level proliferation, immature tetrasporophytes or female and male gametophytes are used as the proliferated mother branches, and after cleaning and cutting, a plurality of independent seedlings are obtained, and then the cut independent seedlings are inoculated into a culture container containing culture solution to perform suspension aeration culture, to promote the recovery, budding and growth of the algae seeds. During the second-level proliferation, the first-level proliferated red seaweed seedlings are inoculated into a floating frame with holes and placed in a circulating water seedling proliferation pond for culture, to make the seedlings grow rapidly and achieve the effect of rapid proliferation.

[0046] The whole set of red feather algae seedling propagation method provided by the present application combines the primary propagation and secondary propagation processes, and the primary propagation alone cannot achieve good results, has space limitations, slower growth, longer time, lower efficiency, and more complicated management of replacing culture solution, and is prone to breeding of miscellaneous algae. The primary propagation of the present application aims to: (1) keep the seedlings all year round, which can avoid the limitation of low temperature period in winter; (2) slowly propagate, which is convenient for tracking growth data; (3) recover the wound of the algae seedlings after splitting; and (4) use small-sized seedlings, which can split more seedlings from limited algae seedlings, and grow the smaller seedlings to a certain size applicable to secondary propagation. The secondary propagation space and environment are larger, which is conducive to the rapid growth of seedlings and realizes rapid propagation.

[0047] The propagation method provided by the present application is completed in a land-based artificial regulation environment, has the advantages of high propagation efficiency, short cultivation period, high seedling survival rate and high budding rate, and the survival rate and budding rate are as high as 89% to 100%, and the red feather algae seedlings of uniform size reaching the suitable size can be cultured after 10 to 12 days. Therefore, the propagation method provided by the present application is simple and efficient, and a large amount of high-quality red feather algae seedlings can be obtained in a short time, which not only solves the problem of the source of red feather algae seedlings to a certain extent, but also has stable growth, low mortality and good repeated propagation ability, can be repeatedly used for step-by-step propagation, fully improves the utilization rate of seedlings, and is conducive to the rapid and stable increase of seedling scale.

[0048] In addition, the propagation method provided by the present application has low requirements for equipment conditions, low construction and operation cost, high controllability, good operability and environmental adaptability. Through propagation in an artificial regulation environment, seedling raising and seedling keeping can be realized all year round, effectively solving the problems of seedling keeping, propagation and breeding in winter low temperature, and reducing the seasonal limitation. The propagated red feather algae seedlings can be directly applied to land-based factory propagation or breeding, which avoids the risk of uncontrollable factors in sea area breeding on the one hand, and the long span of suitable breeding period of land-based factory on the other hand, so the propagation method of the present application also prolongs the suitable breeding period, and has good popularization and application prospect.

[0049] It should be noted that the first expansion needs to comprehensively consider the wound recovery of the split seedlings, the length of the seedlings and the contamination of the algae to end the process. The seedlings are too small to be suitable for the expansion mode of the second expansion, and the wound has not recovered, which is also easy to cause high mortality of the seedlings in the second expansion. At the same time, the long expansion time is easy to breed algae, and the slow growth will also lead to the expansion efficiency, and the environmental and space factors will also lead to growth restriction, affecting the expansion speed of the seedlings. In the technical scheme of the present application, it is observed that the size of the seedlings reaches 3-5 cm after the first expansion, and the first expansion of the red ling algae seedlings obtained at this time can be stopped. The seedlings can be cut and divided for a new round of suspension and aeration expansion (first expansion), and can also be used for subsequent land-based circulating water rapid expansion (second expansion). When the seedlings of the second expansion protrude from the seedling net by 5-8 cm, they can be transported back to the algae culture room for suspension and aeration expansion (first expansion) or seedling preservation, or they can be directly inoculated for land-based circulating water scale cultivation.

[0050] In addition, the technical scheme of the present application does not make specific restrictions on the culture container, and a transparent culture container can be selected, such as a conical bottle, a glass bottle or a transparent storage box.

[0051] In the embodiment of the present application, in step S1, the red ling algae includes any one of Pacific red ling and fine weak red ling.

[0052] In the embodiment of the present application, in step S1, the cleaning step includes: washing the surface of the algae body with a brush, then soaking in fresh water for 10-20 s to remove the surface algae, and then washing with sterilized seawater for 2-3 times. The surface of the algae body may be contaminated by epiphytic algae, and the algae body is cleaned in order to keep the red ling algae seedlings from being damaged and to make the used algae material as clean as possible to reduce the pollutants or competitive organisms that may affect the expanded seedlings.

[0053] In the embodiment of the present application, in step S2, the length of the independent seedlings is 1-2 cm. The technical scheme of the present application cuts the red ling algae seedlings with sterilized scissors or a guillotine to control the length of the divided seedlings to be 1-2 cm, and too short (less than 1 cm) will lead to low survival rate of the algae body, and too long will waste the algae germplasm.

[0054] In the embodiment of the present application, before step S3, the divided independent seedlings are placed in sterilized seawater. Sterilized seawater can significantly reduce the risk of contamination of the seedlings by bacteria, fungi and other microorganisms, and is also helpful to maintain the physiological state of the seedlings and avoid stress caused by environmental mutation.

[0055] In the embodiment of the present application, in step S3, the conditions of the suspension and aeration culture include: water temperature 25-33℃, salinity 25-30, light intensity 30-60 μmol*m -2s -1 .

[0056] The salinity of the culture is too high or too low, which can cause the germination rate of the independent seedlings to decrease. The water temperature is too high or too low, which can cause the germination rate of the independent seedlings to decrease, especially when the temperature is lower than 18℃, the independent seedlings hardly germinate. The light intensity is too low, which can cause the seedlings to grow slowly, and the light intensity is too high, which is easy to cause the seedlings to be polluted by mixed algae, and cause the seedlings to be lost. The technical scheme of the present application sets the conditions of the suspension aeration culture in the above range, which is conducive to providing a suitable growth environment and promoting the germination and growth of the red seaweed seedlings.

[0057] In the embodiment of the present application, in step S3, the culture solution comprises F / 2 culture medium and filtered seawater mixed according to a volume ratio of (0.5-1.5):1000, and the amount of the culture solution is calculated according to 5L of culture solution per 100-250g of independent seedlings.

[0058] It should be noted that the F / 2 culture medium is used to promote the growth of the red seaweed seedlings, and other culture media can also be used instead. On the other hand, although the addition of the culture medium can promote the growth of the seedlings to some extent, it can also cause the corresponding mixed algae to grow rapidly, so the amount of addition needs to be comprehensively considered in combination with the growth rate of the seedlings and the pollution situation of the mixed algae.

[0059] In the embodiment of the present application, in step S3, the pumped gas is gas filtered by a 50mm air filter membrane during the process of the suspension aeration culture. The untreated air can contain bacteria, fungal spores and other microorganisms, and the use of the air filter membrane can effectively remove these microorganisms, reduce the risk of pollution, and reduce the adverse effects of these microorganisms on the growth of the algae.

[0060] In the embodiment of the present application, in step S3, the culture solution and the culture container are replaced every 8-10 days. With the passage of time, the nutrient components in the culture solution will be absorbed and utilized by the algae, and regular replacement of the culture solution can supplement the necessary nutrients to ensure that the algae have enough nutrients to support their rapid growth; by replacing the culture solution, metabolic waste produced in the process of metabolism of the algae can be effectively removed, and the water quality can be maintained clean; regular replacement of the culture solution can also prevent the accumulation of pathogens and pollutants; with the passage of time, the container is easy to accumulate mixed algae, which affects the growth of the seedlings, and regular replacement of the culture container can help to reduce the mixed algae.

[0061] In the embodiment of the present application, in step S4, the mesh diameter of the seedling fixing net is 1-1.5 cm. Too small mesh diameter is easy to be blocked by fouling organisms, affecting the smoothness of water flow, and also blocking the light, which is not conducive to the upward growth of seedlings, and interfering with the normal growth environment of red feather algae seedlings. Too large mesh diameter cannot effectively fix smaller seedlings, and is easy to cause seedlings to fall off. Setting the mesh diameter of the seedling fixing net to 1-1.5 cm has little effect on the normal growth of red feather algae seedlings, and can effectively fix the seedlings.

[0062] In the embodiment of the present application, in step S4, the coverage density of the primary expanded red feather algae seedlings in the floating frame with holes is 1-1.5 kg / m 2 . When the coverage density is lower than 1 kg / m 2 , the algae is too sparse, the cultivation period is prolonged, it is easy to be infected with miscellaneous algae, and the cultivation space is wasted; when the coverage density is higher than 1.5 kg / m 2 , the algae is too dense and overlaps and extrudes each other, wasting the algae.

[0063] In the embodiment of the present application, in step S4, the rapid expansion conditions include: water temperature 20-33℃, salinity 25-30, light intensity 60-120 μmol*m -2 *s -1 .

[0064] Too high or too low salinity is not conducive to the growth of seedlings, affects the germination of seedlings, and seriously causes seedlings to rot. Too high or too low water temperature will make the seedlings grow stagnantly, especially when the temperature is lower than 18℃ for a long time, which will cause the seedlings to die. Too low light intensity will cause the seedlings to grow slowly, and too high light intensity will make the seedlings yellow, and long-term high light will even cause photo damage and lead to whitening.

[0065] In the embodiment of the present application, the circulating water seedling expansion tank is a land-based pool, and the water in and out of the circulating water seedling expansion tank is sand-filtered seawater. The primary red feather algae seedlings expanded by the expansion method of the present application can be directly applied to land-based factory expansion or cultivation, on the one hand, avoiding the risk of uncontrollable factors in sea area cultivation, and on the other hand, the suitable cultivation period of land-based factory is longer, and it can be cultivated from March to November, so the suitable cultivation period can be prolonged.

[0066] In the embodiment of the present application, the circulating water seedling expansion tank comprises: a plurality of 4*5*1.5m 3The seedling propagation pond, the water storage and sedimentation pond, the three-layer sand filtration system, the microfilter, the pipeline system and the light-adjusting sunshade net. When working, the seawater flowing out of the three-layer sand filtration system is first injected into the water storage and sedimentation pond, and after sedimentation, sterilization and disinfection, the seawater in the water storage and sedimentation pond is pumped into the seedling propagation pond through the microfilter, and the seawater in the seedling propagation pond flows into the water storage and sedimentation pond through the water outlet pipeline, so as to form a circulating loop. The whole secondary propagation and expansion process maintains the seawater amount in the cultivation system unchanged, and the seawater is in a dynamic flow process. The seawater dynamic circulation utilization increases the flowability of seawater, promotes the growth of seaweed by water flow, and improves the seawater utilization rate. In an embodiment of the present application, the circulating water propagation pond is a land-based factory cement pond, which can normally import and export seawater and be irradiated by natural light.

[0067] The seedling propagation pond provides cultivation space for seedling growth; the water storage and sedimentation pond ensures the cleanliness and stability of water quality by standing; the three-layer sand filtration system can remove large-particle pollutants and miscellaneous algae in seawater; the microfilter includes a microfiltration membrane, in the microfiltration process, the solution passes through a porous microfiltration membrane (i.e. microfiltration membrane), the pore size of the microfiltration membrane ranges from 0.1 to 10 microns, which can effectively intercept particles, bacteria, microorganisms and some macromolecules in the solution, but allows water and small molecule solutes to pass through; the light-adjusting sunshade net is used to regulate the natural light between 60-120 μmol*m -2 *s -1 .

[0068] In an embodiment of the present application, before step S4, the following steps are included:

[0069] Disinfecting the circulating water seedling propagation pond.

[0070] The land-based circulating water cement pond is sterilized with high-concentration bleaching powder or chlorine tablets in advance to reduce the risk of pollution and reduce the adverse effects of some microorganisms on the growth of algal plants.

[0071] The technical solutions of the present application will be further described in detail below in combination with specific embodiments and drawings. It should be understood that the following embodiments are only used to explain the present application and not to limit the present application.

[0072] Embodiment 1

[0073] First-stage propagation experiment of red seaweed seedlings:

[0074] The first-stage propagation experiment was carried out in the algae cultivation laboratory in Longwangwan Park, Potou District, Zhanjiang, Guangdong. All consumables were sterilized and cleaned in advance. The steps of the first-stage propagation experiment include:

[0075] (1) Algal species preparation: immature fine red wing four spore body is selected, the surface of the algal body is washed with a brush, and the surface miscellaneous algae is removed by rapid soaking in fresh water for 20s, and then the algal body is repeatedly washed with sterilized seawater for 3 times to obtain the propagation mother body.

[0076] (2) Seedling separation: the propagation mother body is cut and separated by using a sterilized scissors, and the length of the separated seedling is set as different separation lengths (0.2cm, 0.5cm, 1cm, 1.5cm and 2cm) to obtain independent seedlings. The cut seedlings are quickly placed in sterilized seawater for use.

[0077] (3) Propagation mode: the cut seedlings are inoculated in a transparent glass for suspension and aeration culture, wherein the aeration gas is filtered through a 50mm air filter membrane; the transparent glass is in a sealed state; the seedling inoculation density is 23g / L; 115g independent seedlings are added into 5L culture solution, the culture solution is filtered seawater (1mL culture medium: 1L filtered seawater) added with F / 2 culture medium, and the culture solution and culture container are replaced every 10 days. The suspension and aeration culture conditions are as follows: different water temperatures (17℃, 21℃, 25℃, 29℃ and 33℃), different artificial light intensities (30μmol*m -2 *s -1 , 60μmol*m -2 *s -1 and 120μmol*m -2 *s -1 ), different salinities (15, 20, 25, 30, 35 and 40), and a culture period of 10d. For each group of different seedling lengths, different salinities, different light intensities and different temperatures, 3 sections are set, and 3 parallel groups are set. The budding and growth results of the red wing seedlings in each group are observed, and the results are shown in Table 1, Figures 1 to 5 .

[0078] Table 1 Budding and growth of red wing algae under different culture conditions for 10d

[0079]

[0080] According to the results in Table 1 and Figures 1 to 5 , it can be known that:

[0081] (1) When the seedling length is too short, the survival rate of the algal body is low, and the budding rate is also low. It is more appropriate to control the length of the separated seedling to be 1-2cm.

[0082] (2) Different temperatures, different light intensities and different salinities have significant effects on the growth of the red wing algae. The suitable temperature range for the growth of the red wing algae is 25-33℃, and the suitable light intensity range is 30-60μmol*m -2 *s -1The salinity range is 25-30, and the survival rate and budding rate of the algal body are higher.

[0083] Example 2

[0084] Secondary propagation experiment of red seaweed seedlings:

[0085] The secondary propagation experiment (land-based circulating water rapid propagation) was carried out in the Marine Biology Research Base of Guangdong Ocean University. The circulating water seedling propagation pool system was built, and the circulating water seedling propagation pool was disinfected clean in advance with high-concentration bleaching powder. The method for carrying out the secondary propagation experiment comprises the following steps:

[0086] The seedlings (seedling size is 3-5 cm) of example 1 which have been subjected to primary propagation are uniformly inoculated into a rectangular floating frame with holes (size is 40 cm*30 cm*30 cm) ( Figure 6 ), and the seedlings are fixed with a seedling fixing net with a mesh diameter of 1 cm. The seedling fixing net is fixed on the upper layer of the bottom of the floating frame through a 10 cm long fixing belt, and the floating frame is put into the circulating water seedling propagation pool for propagation. During the propagation, the circulating water is kept running and the water body is aerated, the natural light is controlled between 60-120 μmol*m -2 *s -1 , the water temperature is controlled at 20-33℃, the salinity is controlled at 25-30, and the propagation period is 30 days. The growth conditions of the red seaweed are shown in table 2 and Figure 7 .

[0087] Table 2 Growth conditions of red seaweed after primary propagation and secondary propagation

[0088] Mode of propagation Primary propagation Secondary propagation Temperature / °C 25～33 20～33 Illumination / μmol m -2 *s -1 ]]> 30～60 60～120 Salinity 25～30 25～30 Time / days 10 30 Average initial weight / g 115 120 Inoculation density 23 g / L 1000 g / m 2 ]] Average final weight / g 142.5 539 Daily growth rate 0.55 g / (D*L) 116.4 g / (D*m 2 )]]> Weight gain rate / % 24 349

[0089] According to the results of table 2 and Figure 7 , after 30 days of culture, the average final weight of the red seaweed seedlings is 539 g, the daily growth rate of the red seaweed seedlings reaches 116.4 g / (D*m 2 ), and the weight gain rate is 349%.

[0090] The above only describes exemplary embodiments of the present application, and does not limit the patent scope of the present application, and any equivalent structural transformation made according to the technical concept of the present application, or direct / indirect application in other related technical fields is included in the patent protection scope of the present application.

Claims

1. A method for rapid propagation of seedling of Bangia fuscopurpurea, characterized by, The method comprises the following steps: S1, providing immature tetrasporophytes or gametophytes of red algae, and cleaning the algae to remove surface impurities to obtain propagation mother bodies; S2, cutting and separating the propagation mother bodies to obtain independent seedlings; S3, inoculating the independent seedlings into a culture container containing culture solution, and performing suspension and aeration culture until the length of the independent seedlings reaches 3-5 cm to obtain first-level propagated red algae seedlings; S4, inoculating the first-level propagated red algae seedlings into a floating frame with holes, fixing the upper end of the first-level propagated red algae seedlings with a seedling fixing net, and then placing the inoculated floating frame with holes into a circulating water seedling propagation pool for rapid propagation until the length of the seedlings exceeds the seedling fixing net by 5-8 cm to obtain second-level propagated red algae seedlings.

2. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S1: The red algae includes any one of Pacific red algae and weak red algae; and / or, The cleaning step includes: brushing the surface of the algae with a brush, then soaking in fresh water for 10-20 s to remove surface impurities, and then washing with sterilized seawater for 2-3 times.

3. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S2, the length of the independent seedlings is 1-2 cm.

4. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S3: The conditions of the suspension aerated culture include: water temperature 25-33℃, salinity 25-30, light intensity 30-60 μmol*m -2 *s -1 .

5. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S3: The culture solution includes F / 2 culture medium and filtered seawater mixed at a volume ratio of (0.5-1.5):1000, and the amount of the culture solution is calculated as 5 L of culture solution per 100-250 g of independent seedlings; and / or, In the process of suspension and aeration culture, the pumped gas is air filtered through a 50 mm air filter membrane.

6. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S4, the mesh diameter of the seedling fixing net is 1-1.5 cm.

7. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S4, the primary propagated Porphyra haitanensis seedlings have a covering density of 1-1.5 kg / m in the perforated floating frame. 2 .

8. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. In step S4, the conditions for the rapid propagation include: water temperature 20-33℃, salinity 25-30, light intensity 60-120 μmol*m -2 *s -1 .

9. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. The circulating water seedling propagation pool is a land-based pool, and the water entering and exiting the circulating water seedling propagation pool is sand-filtered seawater.

10. The method of claim 1, wherein the red seaweed seeds are propagated rapidly. Before step S4, it includes: Disinfecting the circulating water seedling propagation pool.

Images