Application of hippophae rhamnoides extract in preparation of products for preventing and treating cognitive dysfunction and / or emotional disorder and improving memory
By extracting sea buckthorn fruit oil, sea buckthorn seed oil, and sea buckthorn pulp from sea buckthorn fruit and seeds, the problem of depression, cognitive impairment, and memory decline caused by metabolic disorders induced by high-calorie diets has been addressed, providing a cost-effective alternative treatment with fewer side effects.
Patent Information
- Application Number
- CN202511322359.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-16
- Publication Date
- 2025-11-11
AI Technical Summary
In existing technologies, the problems of depression, cognitive impairment and memory decline caused by metabolic disorders induced by high-calorie diets have issues with poor long-term dependence and significant side effects when treated with drugs.
Seabuckthorn fruit oil, seabuckthorn seed oil, and seabuckthorn pulp are extracted from seabuckthorn fruit and seeds using supercritical carbon dioxide extraction. These are used as nutrient-rich natural foods to prevent and treat cognitive and mood disorders and improve memory.
Sea buckthorn extract has shown significant preventive and therapeutic effects with few side effects, is economical, and is suitable for long-term use. It can replace traditional drug treatments and improve mood disorders, cognitive impairment, and memory decline associated with metabolic disorders caused by high-calorie diets.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of biomedical technology, specifically relating to the application of sea buckthorn extract in the preparation of products for the prevention and treatment of cognitive impairment and / or mood disorders, as well as for improving memory. Background Technology
[0002] Mood disorders are a class of mental illnesses primarily characterized by abnormal mood. Their core features include problems with the intensity, duration, or regulation of mood, impacting daily life and social functioning. Common types include depression, bipolar disorder, and anxiety disorders. Depression is a mental illness characterized by low mood, slowed thinking, decreased motivation, and even pessimism, suicidal ideation, and other clinical symptoms. It is most commonly comorbid with obesity, metabolic disorders (MetD) such as diabetes, and cardiovascular disease, with a high-calorie diet being a significant contributing factor to metabolic disorders. A series of reviews published in the Lancet Diabetes Endocrinol in 2020 indicated that stroke, dementia, and depression are clinical complications of high-calorie diets, and that individuals with a long-term high-calorie diet and those who have experienced acute stressful events have a 1.5-2.0 times increased risk of developing major depressive disorder. In recent years, with the advancement of medical research, the close link between depression and energy metabolism has gradually gained attention. Numerous clinical studies have found that patients with depression often have multiple metabolic abnormalities, such as glucose metabolism disorders, lipid metabolism abnormalities, and energy metabolism imbalances. These metabolic abnormalities are not only accompanying phenomena of depression, but may also play a key role in the occurrence and development of depression, and may even be one of the core driving factors of depression.
[0003] Depression is primarily treated with medication, but long-term medication use and recurring symptoms can easily lead to cognitive impairment. Cognitive impairment refers to abnormalities in the brain's processes of acquiring, processing, storing, and applying information, which can manifest as memory loss, poor concentration, slow thinking, difficulty in language comprehension or expression, and impaired executive functions (such as decision-making and planning). Currently, with the aging of the global population, the prevalence of cognitive impairment diseases such as Alzheimer's disease is increasing year by year. Existing treatments for metabolic disorders caused by high-calorie diets mainly involve medication and health management, while treatments for depression, cognitive impairment, and memory loss primarily involve medication and psychological adjustment. Both types of drug treatments are characterized by long durations, poor dependence, and significant side effects. Summary of the Invention
[0004] In view of this, the purpose of the present invention is to provide an application of sea buckthorn extract or products containing sea buckthorn extract in the preparation of products for the prevention and treatment of cognitive impairment and / or mood disorders and for improving memory. This provides a natural food rich in nutrients as a substitute for traditional drug treatment, with fewer side effects, economic benefits, suitability for long-term use, good dependence, and significant preventive and therapeutic effects.
[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution:
[0006] This invention provides the application of sea buckthorn extract or products containing sea buckthorn extract in the preparation of products for preventing and treating cognitive impairment and / or mood disorders and improving memory, wherein the sea buckthorn extract includes sea buckthorn fruit oil, sea buckthorn seed oil and / or sea buckthorn pulp.
[0007] Preferably, the mood disorder includes depression.
[0008] Preferably, the depression is depression caused by a high-calorie diet.
[0009] Preferably, the preparation methods of the sea buckthorn fruit oil and sea buckthorn fruit pulp include the following steps: crushing the sea buckthorn fruit, extracting it using supercritical carbon dioxide extraction, centrifuging it, and obtaining sea buckthorn fruit oil in the upper layer; the preparation method of the sea buckthorn fruit pulp includes the following steps: crushing the sea buckthorn fruit into a pulp, grinding it, passing it through a 90-mesh sieve, collecting the sieved material, and obtaining sea buckthorn fruit pulp.
[0010] Preferably, the supercritical carbon dioxide extraction method has an extraction temperature of 30–60°C, a pressure of 10–30 MPa, and a CO2 flow rate of 5–20 L / h.
[0011] Preferably, the centrifugation speed is 3000-6000 rpm and the time is 15-40 min.
[0012] Preferably, the preparation method of the sea buckthorn seed oil includes the following steps: crushing the sea buckthorn seeds, extracting them using supercritical carbon dioxide extraction, separating the solid and liquid components, and obtaining sea buckthorn seed oil.
[0013] Preferably, the supercritical carbon dioxide extraction method has an extraction temperature of 35–60°C, a pressure of 15–35 MPa, and a CO2 flow rate of 8–25 L / h.
[0014] Preferably, the particle size of the pulverized material is 0.5 to 2 mm.
[0015] Preferably, the solid-liquid separation method includes centrifugation, wherein the centrifugation speed is 4000-8000 rpm and the time is 15-40 min.
[0016] The beneficial effects of this invention are:
[0017] The sea buckthorn extracts (sea buckthorn pulp, sea buckthorn fruit oil, and sea buckthorn seed oil) provided by this invention all have the effect of preventing and / or treating cognitive impairment and / or mood disorders, and improving memory. The sea buckthorn extracts of this invention are prepared from sea buckthorn, a natural food rich in nutrients. They have significant preventive and therapeutic effects on cognitive impairment and / or mood disorders, can replace traditional drug treatments, and can improve memory. They also have the advantages of few side effects, being economical, suitable for long-term use, and having good patient compliance. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of the supercritical carbon dioxide extraction device of the present invention, wherein ① is the extraction vessel, ② is the separation column, ③ is the heat exchanger, ④ is the condenser, ⑤ is the cryogenic cooler, ⑥ is the pump, ⑦ is the heat exchanger, and ⑧ is the storage tank.
[0019] Figure 2 The figures show the metabolic disturbances caused by a high-calorie diet in Example 7. Figure A shows the weight change, Figure B shows the random blood glucose concentration test results, Figure C shows the fasting blood glucose concentration test results, Figure D shows the line graph of blood glucose concentration changes in the ITT experiment, Figure E shows the standardized blood glucose concentration curve in the ITT experiment, Figure F shows the area under the curve of the standardized blood glucose concentration curve in the ITT experiment, Figure G shows the line graph of blood glucose concentration changes in the OGTT experiment, and Figure H shows the area under the curve of the line graph of blood glucose concentration changes in the OGTT experiment.
[0020] Figure 3 The results for the depressive symptoms of mice in each group in Example 8 are shown. A is the activity trajectory of the opening experiment, B is the route map of the elevated cross maze experiment, C, D and E are the statistical results of the total movement distance, the number of times entering the central area and the number of times standing in the opening experiment, respectively, F, G and H are the statistical results of the total movement distance, the number of times entering the open arm and the dwell time in the open arm, respectively, I is the statistical result of the movement distance around the new object, and J is the statistical result of the number of times around the new object. Detailed Implementation
[0021] This invention provides the application of sea buckthorn extract or products containing sea buckthorn extract in the preparation of products for preventing and treating cognitive impairment and / or mood disorders and improving memory, wherein the sea buckthorn extract includes sea buckthorn fruit oil, sea buckthorn seed oil and / or sea buckthorn pulp.
[0022] In this invention, the mood disorder preferably includes depression; the depression is preferably depression caused by a high-calorie diet. In some embodiments of this invention, the cognitive impairment and mood disorder are metabolic disorder-related mood disorders and cognitive impairments caused by a high-calorie diet. This invention does not specifically limit the source of sea buckthorn; conventional commercially available products in the art are acceptable. In this invention, the product preferably includes a drug. In this invention, the product containing sea buckthorn extract preferably includes a drug, food, or health product.
[0023] In this invention, the preferred method for preparing sea buckthorn fruit oil and sea buckthorn fruit pulp includes the following steps: crushing sea buckthorn fruit, extracting it using supercritical carbon dioxide extraction, centrifuging it, and obtaining sea buckthorn fruit oil in the upper layer; the method for preparing sea buckthorn fruit pulp includes the following steps: crushing sea buckthorn fruit into a pulp, grinding it, passing it through a 90-mesh sieve, collecting the sieved material, and obtaining sea buckthorn fruit pulp.
[0024] This invention does not specifically limit the method for crushing sea buckthorn berries. In some embodiments of this invention, the crushing method is mechanical pressing, grinding, or crushing. In this invention, when the crushed sea buckthorn berries are extracted using supercritical carbon dioxide extraction, the extraction temperature is preferably 30–60°C, more preferably 40–55°C, and even more preferably 45–52°C; the extraction pressure is preferably 10–30 MPa, more preferably 15–25 MPa, and even more preferably 18–22 MPa; the CO2 flow rate is preferably 5–20 L / h, more preferably 8–18 L / h, and even more preferably 9–15 L / h. In this invention, after extraction, the extract is collected and centrifuged. The centrifugation speed is preferably 3000–6000 rpm, more preferably 3800–5800 rpm, and even more preferably 4500–5500 rpm; the centrifugation time is preferably 15–40 min, more preferably 20–35 min, and even more preferably 25–32 min. In the preparation method of sea buckthorn pulp of the present invention, fresh sea buckthorn berries are preferably washed, dehydrated, and pulped into a pulp using a pulping machine; then ground in a colloid mill for 4-6 minutes to achieve a pulp fineness of 90 mesh. In this invention, small-fruited sea buckthorn from Ningxia, Qinghai, and Gansu is preferred, and frost-damaged berries are selected (for more complete sugar conversion). Metal containers are avoided (as they will destroy vitamin C). The entire process is carried out in the dark (to prevent oxidation of nutrients), and the temperature is controlled to not exceed 40°C (to protect active enzymes).
[0025] In this invention, the preferred method for preparing sea buckthorn seed oil includes the following steps: crushing sea buckthorn seeds, extracting them using supercritical carbon dioxide extraction, separating the solid and liquid phases, and obtaining sea buckthorn seed oil.
[0026] This invention does not specifically limit the method of pulverizing sea buckthorn seeds; any conventional pulverizing method in the art can be used. In this invention, it is preferred to pulverize the sea buckthorn seeds to a particle size of 0.5–2 mm. In some embodiments of this invention, the particle size is 0.5 mm, 0.8 mm, 1.0 mm, 1.3 mm, 1.6 mm, 1.8 mm, or 2 mm. In this invention, when using supercritical carbon dioxide extraction to extract the pulverized sea buckthorn seeds, the extraction temperature is preferably 35–60°C, more preferably 40–58°C, and even more preferably 48–56°C; the extraction pressure is preferably 15–35 MPa, more preferably 18–32 MPa, and even more preferably 20–30 MPa; the CO2 flow rate is preferably 8–25 L / h, more preferably 10–20 L / h, and even more preferably 13–18 L / h. In this invention, the solid-liquid separation method preferably includes centrifugation, the centrifugation speed is preferably 4000-8000 rpm, more preferably 5000-7000 rpm, and even more preferably 5500-6500 rpm; the centrifugation time is preferably 15-40 min, more preferably 20-35 min, and even more preferably 25-32 min.
[0027] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0028] Unless otherwise specified, the following embodiments are all conventional methods.
[0029] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.
[0030] Example 1
[0031] Preparation of sea buckthorn fruit oil:
[0032] Sea buckthorn fruit oil was prepared using supercritical carbon dioxide extraction, and a schematic diagram of the supercritical carbon dioxide extraction apparatus used is shown below. Figure 1 As shown, the specific preparation method is as follows:
[0033] After crushing the sea buckthorn berries, they were placed in an extraction vessel, and the required extraction temperature was set to 50℃. CO2 from the storage tank was pressurized by a pump and preheated to the set temperature of 50℃ in a heat exchanger. The inlet valve was opened to allow the CO2 fluid to enter the extraction vessel and contact the crushed sea buckthorn berries. The CO2 flow rate was controlled at 10 L / h. Under the set extraction temperature of 50℃ and pressure of 20 MPa, the sea buckthorn oil contained in the berries was fully dissolved in the supercritical CO2. After a residence time of 25 minutes, the product was discharged. The sea buckthorn oil and CO2 were separated in a separation column. The separated product was collected after being discharged through a vent valve. The CO2 gas was recycled back to the storage tank after passing through a heat exchanger, condenser, and cryostat. Recycling was stopped when almost no product could be collected at the vent valve. The product collected from the vent valve was centrifuged at 5000 rpm for 30 minutes; the upper layer was sea buckthorn oil.
[0034] Example 2
[0035] Preparation of sea buckthorn fruit oil: The difference from Example 1 is that the supercritical carbon dioxide extraction temperature is 30℃, the pressure is 10MPa, the CO2 flow rate is 5L / h, and the centrifugation conditions are 3000rpm for 40min. All other conditions are the same as in Example 1.
[0036] Example 3
[0037] Preparation of sea buckthorn fruit oil: The difference from Example 1 is that the supercritical carbon dioxide extraction temperature is 60℃, the pressure is 30MPa, the CO2 flow rate is 20L / h, and the centrifugation conditions are 6000rpm for 15min. All other conditions are the same as in Example 1.
[0038] Example 4
[0039] Preparation of sea buckthorn seed oil:
[0040] Sea buckthorn seed oil is prepared using supercritical carbon dioxide extraction, and a schematic diagram of the supercritical carbon dioxide extraction apparatus used is shown below. Figure 1 As shown, the specific preparation method is as follows:
[0041] Sea buckthorn seeds were crushed to a particle size of 1 mm and then placed into an extraction vessel. The required extraction temperature was set to 55°C. CO2 from the storage tank was pressurized by a pump and preheated to the set temperature of 55°C in a heat exchanger. The inlet valve was opened to allow the CO2 fluid to enter the extraction vessel and contact the crushed sea buckthorn seeds. The CO2 flow rate was controlled at 15 L / h. At the set extraction temperature of 55°C and pressure of 25 MPa, the sea buckthorn seed oil contained in the sea buckthorn seeds was fully dissolved in the supercritical CO2. After a residence time of 25 min, the product was discharged. The sea buckthorn seed oil and CO2 were separated in a separation column. The separated product was discharged through the vent valve and collected. The CO2 gas was recycled back into the storage tank after passing through a heat exchanger, condenser, and cryostat. The recycling was stopped when almost no product could be collected at the vent valve. The product collected from the vent valve was centrifuged at 6000 rpm for 30 min. The upper layer was sea buckthorn seed oil.
[0042] Example 5
[0043] Preparation of sea buckthorn seed oil: The difference from Example 4 is that the sea buckthorn seeds are crushed to a particle size of 0.5 mm, the supercritical carbon dioxide extraction temperature is 35 °C, the pressure is 15 MPa, the CO2 flow rate is 8 L / h, and the centrifugation conditions are 4000 rpm for 40 min. All other conditions are the same as in Example 4.
[0044] Example 6
[0045] Preparation of sea buckthorn seed oil: The difference from Example 4 is that the sea buckthorn seeds are crushed to a particle size of 2 mm, the supercritical carbon dioxide extraction temperature is 60°C, the pressure is 35 MPa, the CO2 flow rate is 25 L / h, and the centrifugation conditions are 8000 rpm for 15 min. All other conditions are the same as in Example 4.
[0046] Example 7
[0047] Preparation of sea buckthorn pulp: Wash fresh sea buckthorn berries, dehydrate them, and grind them into pulp using a pulping machine; then grind them in a colloid mill for 6 minutes to make the pulp fineness reach 90 mesh, pass it through a 90 mesh sieve, collect the sieved material, and obtain sea buckthorn pulp.
[0048] Example 8
[0049] Forty mice were evenly distributed into five cages, with eight mice in each cage, forming a control group (NCD, n=8) and a model group (Model, n=32). Except for the NCD group, all mice in the Model group were fed a high-fat diet for 16 consecutive weeks.
[0050] Random blood glucose concentration testing: No fasting or water restriction is required before testing. Mice are normally fed, and after calibration of the blood glucose meter, gentle fixation, and tail tip disinfection, blood is collected and the reading reflects the immediate blood glucose level. Fasting blood glucose testing: Mice need to be fasted overnight for 12 hours (water is not restricted). The instrument is calibrated, the mice are gently fixed, and blood is collected after tail tip treatment.
[0051] OGTT experiment: Mice were fasted overnight for 12 hours (water was not restricted), and after fasting blood glucose was measured, they were administered 20% glucose solution by gavage at a dose of 1 g / kg body weight. Blood was collected from the tail tip at 15, 30, 60 and 120 minutes after gavage to measure blood glucose values. The data were recorded and the area under the blood glucose curve (AUC) was calculated to assess glucose tolerance.
[0052] ITT experiment: Mice were fasted for 4 hours, and their basal blood glucose was measured. Insulin solution was injected intraperitoneally at a dose of 1 U / kg body weight. Blood glucose was measured at 15, 30, 60, and 120 minutes after injection. The rate of decrease in blood glucose was calculated, and insulin sensitivity was assessed.
[0053] The results are as follows Figure 2 As shown, during and after the high-fat diet, the model mice in each group showed a significant increase in body weight. Figure 2 A in the text indicates elevated blood sugar levels. Figure 2 (B and C in the text), the ITT experiment showed that the model mice had insulin resistance ( Figure 2 The D, E, and F values in the text indicate that the OGTT experiment showed that the model mice exhibited oral glucose intolerance (D, E, and F). Figure 2 The presence of G and H in the data indicates that a high-energy diet can cause metabolic disorders in the body.
[0054] Example 9
[0055] The normal control mice from Example 8 were used as the control group (NCD group). The Model group mice were divided into four subgroups: a model group (high-calorie diet-induced metabolic disorder-related mood and cognitive impairment group), a sea buckthorn fruit oil group (fruit oil group), a sea buckthorn fruit pulp group (fruit pulp group), and a sea buckthorn seed oil group (seed oil group). Except for the blank control group and the model group, mice in the sea buckthorn fruit oil group, sea buckthorn fruit pulp group, and sea buckthorn seed oil group were administered sea buckthorn fruit oil prepared in Example 1, sea buckthorn fruit pulp prepared in Example 7, and sea buckthorn seed oil prepared in Example 4, respectively, by gavage at a rate of 200 μL / day. Mice in the NCD group and the Model group were administered an equal volume of physiological saline by gavage. After 21 days of gavage treatment, the mice in each group underwent the opening field test, the new object recognition test, and the elevated cruciate maze test to observe the accompanying depressive state of the mice. The experimental methods are as follows:
[0056] In the opening experiment, mice were carefully placed in an open enclosure at the start of the experiment and allowed to explore freely. A camera system was used to record the mice's movement trajectory, speed, and number of times they stood up in real time. The mice's alertness to the environment was observed, including whether they frequently sniffed their surroundings, whether they exhibited rigid postures, and their behavioral preferences in the central and peripheral areas. Observation continued for 5 minutes, during which the environment was kept as quiet as possible to minimize external interference and ensure the accuracy of the experimental data. After the experiment, the open enclosure was cleaned and disinfected to ensure the accuracy of the next experiment.
[0057] In the elevated cross maze experiment, mice were placed in the center of the maze facing the open arms at the start. Behavioral indicators such as the number of times they entered the open and closed arms, the duration of their stay, and the number of squares they traversed were recorded within a certain time period (5 minutes). The mice's exploration behavior towards the open arms reflected their anxiety level; the more times they entered and the longer they stayed in the open arms, the lower their anxiety level. The frequency of urination and defecation was also observed, as these indicators helped determine their emotional state. After the experiment, the maze was cleaned and disinfected to ensure the accuracy of the next experiment.
[0058] New Object Recognition Experiment: First, the animal was placed in an open experimental box and allowed to acclimatize for 10 minutes. Next, in the sample phase, two identical objects were placed in the box, and the animal was allowed to explore freely for 5 minutes, recording the time spent on contact, smelling, and other exploratory behaviors with each object. Then, in the experimental phase, one of the old objects was replaced with a new object, and the animal was placed back in the box, again recording the exploration time for both the old and new objects. The ratio of exploration time for the new and old objects was compared.
[0059] The results are as follows Figure 3 As shown, compared with the blank control group, the model group mice showed no significant difference in total movement distance in the open field test, but significantly reduced entry into the central area and number of times they stood up; in the elevated cross maze test, the total movement distance decreased, the number of times they entered the open arms decreased, and the dwell time in the open arms decreased; in the new object recognition test, the model group mice showed a decrease in both movement distance and number of times they entered the new object, exhibiting obvious signs of depression, cognitive decline, and memory loss. However, after corresponding treatment, the above indicators in the sea buckthorn fruit oil group, sea buckthorn fruit pulp group, and sea buckthorn seed oil group mice all recovered to varying degrees.
[0060] It is evident that the sea buckthorn pulp, sea buckthorn fruit oil, and sea buckthorn seed oil prepared in this invention have good improving and therapeutic effects on mood disorders, cognitive impairment, and memory decline in mice caused by metabolic disorders induced by a high-calorie diet. Among them, sea buckthorn fruit oil and sea buckthorn seed oil have better improving and therapeutic effects and are expected to be used as drugs for treating mood disorders, cognitive impairment, and memory decline caused by metabolic disorders induced by a high-calorie diet, showing good application prospects.
[0061] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. The application of sea buckthorn extract or products containing sea buckthorn extract in the preparation of products for the prevention and treatment of cognitive impairment and / or mood disorders and for improving memory, characterized in that, The seabuckthorn extract includes seabuckthorn fruit oil, seabuckthorn seed oil, and / or seabuckthorn pulp.
2. The application according to claim 1, characterized in that, The mood disorders mentioned include depression.
3. The application according to claim 2, characterized in that, The depression mentioned is depression caused by a high-calorie diet.
4. The application according to claim 1, characterized in that, The preparation method of the sea buckthorn fruit oil includes the following steps: crushing the sea buckthorn fruit, extracting it using supercritical carbon dioxide extraction, centrifuging it, and the upper layer is sea buckthorn fruit oil; the preparation method of the sea buckthorn fruit pulp includes the following steps: crushing the sea buckthorn fruit into a pulp, grinding it, passing it through a 90-mesh sieve, collecting the sieved material, and obtaining sea buckthorn fruit pulp.
5. The application according to claim 4, characterized in that, The supercritical carbon dioxide extraction method has an extraction temperature of 30–60℃, a pressure of 10–30 MPa, and a CO2 flow rate of 5–20 L / h.
6. The application according to claim 4, characterized in that, The centrifugation speed is 3000-6000 rpm, and the time is 15-40 min.
7. The application according to claim 1, characterized in that, The preparation method of the sea buckthorn seed oil includes the following steps: crushing the sea buckthorn seeds, extracting them using supercritical carbon dioxide extraction, separating the solid and liquid components, and obtaining the sea buckthorn seed oil.
8. The application according to claim 7, characterized in that, The supercritical carbon dioxide extraction method has an extraction temperature of 35–60℃, a pressure of 15–35 MPa, and a CO2 flow rate of 8–25 L / h.
9. The application according to claim 7, characterized in that, The particle size of the pulverized material is 0.5–2 mm.
10. The application according to claim 7, characterized in that, The solid-liquid separation method includes centrifugation, wherein the centrifugation speed is 4000-8000 rpm and the time is 15-40 min.