Instrument and method for preparing bile-imitating working fluid

Abstract

The invention provides an instrument and method for preparing bile-imitating working fluid. The instrument comprises a base, and a feeding mechanism and a mixing mechanism are arranged on the base; wherein the mixing mechanism comprises a mixing box arranged on the base, a viscosity sensor, a refractive index detection module, a temperature and density integrated sensor and a dissolved oxygen sensor which are uniformly distributed in the circumferential direction are arranged on the inner side wall of the mixing box, and an ultrasonic defoaming module is arranged at the inner bottom of the mixing box; the base is also provided with a controller which is respectively connected with the viscosity sensor, the refractive index detection module, the temperature and density integrated sensor, the dissolved oxygen sensor and the ultrasonic defoaming module; the method has the beneficial effects that the traditional manual proportioning error can be eliminated, and the viscosity, the refractive index and the density index of each batch of liquid are highly consistent; parameter deviation caused by factors such as temperature and environment humidity in the preparation process can be avoided; and real-time correction can be realized through sensor feedback and automatic adjustment.

Description

Technical Field

[0001] This invention relates to the fields of medical bioengineering and experimental fluid dynamics, and in particular to an instrument and method for preparing a bile-like working solution. Background Technology

[0002] Particle image velocimetry (PIV), as a high-precision, non-contact flow velocity measurement method, has been widely used in in vitro fluid experiments. However, when PIV is applied to the study of biliary system fluids, the biliary system, as an experimental scenario with a narrow diameter, limited transparency, and high requirements for tissue biomimicry, places special demands on the flow medium (bile-like fluid), including: 1) refractive index matching with the model material (such as silica gel) to prevent imaging distortion; 2) controllable viscosity and density similar to real bile to ensure kinetic similarity; 3) air bubbles can cause reflection artifacts in PIV imaging, which need to be removed as much as possible; and 4) temperature stability and environmental adaptability during the experiment.

[0003] Currently, there is a lack of a systematic solution that integrates liquid formulation optimization and real-time physical parameter monitoring. Existing studies mostly use manually prepared liquid formulations, lacking online monitoring of process parameters (such as viscosity and refractive index), making it difficult to ensure consistency and repeatability across different batches. Furthermore, the refractive index of the same batch of prepared solutions is inconsistent under different room temperatures and humidity levels. Therefore, there is an urgent need to develop a controllable, real-time monitoring method for preparing bile-like liquids, along with corresponding preparation instruments. Summary of the Invention

[0004] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide an instrument and method for preparing a bile-like working fluid, which solves the problem that the prior art lacks online monitoring of preparation process parameters (such as viscosity and refractive index), making it difficult to ensure the consistency and repeatability of different batches of liquid.

[0005] To achieve the above and other related objectives, the present invention provides the following technical solution:

[0006] An instrument for preparing a bile-like working fluid includes a base, on which a feeding mechanism and a mixing mechanism located to the side of the feeding mechanism are provided. The feeding mechanism includes a feeding box, which is divided by a partition into a first storage chamber for storing glycerin, a second storage chamber for storing deionized water, and a third and fourth storage chamber for storing a refractive index regulator. The mixing mechanism includes a mixing tank and a stirring assembly mounted on the base. The inner wall of the mixing tank is provided with a viscosity sensor, a refractive index detection module, a temperature and density integrated sensor, and a dissolved oxygen sensor evenly distributed circumferentially. The bottom of the mixing tank is provided with an ultrasonic defoaming module. The base also has controllers connected to the viscosity sensor, refractive index detection module, temperature and density integrated sensor, dissolved oxygen sensor, and ultrasonic defoaming module.

[0007] In one embodiment of the present invention, the top side of the base is provided with a plurality of first drainage holes communicating with the interior of the base, and the front side of the base is also provided with a plurality of second drainage holes communicating with the interior of the base.

[0008] In one embodiment of the present invention, the top of the first storage cavity, the second storage cavity, the third storage cavity and the fourth storage cavity are all provided with a cover plate. The feeding mechanism further includes a water pump, a first guide pipe and a second guide pipe installed on the side wall of the feeding box. One end of the first guide pipe extends into the storage cavity, and the other end of the first guide pipe passes through the cover plate and is connected to the inlet of the water pump. One end of the second guide pipe is connected to the outlet of the water pump, and the other end of the second guide pipe extends into the mixing box.

[0009] In one embodiment of the present invention, the mixing mechanism further includes a cover mounted on the mixing chamber, and the stirring assembly includes a drive motor mounted inside a protective shell and a first stirring rod connected to the output shaft of the drive motor. The end of the first stirring rod away from the drive motor extends through the protective shell and the cover into the mixing chamber, and the surface of the first stirring rod is provided with a plurality of second stirring rods located inside the mixing chamber.

[0010] In one embodiment of the present invention, the base is further provided with a support assembly movably connected to the protective shell. The support assembly includes a first support frame mounted on the base and a second support frame movably connected to the first support frame. The second support frame is also movably connected to the protective shell. An ambient temperature sensor and an ambient humidity sensor are provided on the first support frame. The drive motor, the ambient temperature sensor, and the ambient humidity sensor are also connected to the controller.

[0011] A method for preparing a bile-like working solution, based on the aforementioned bile-like working solution preparation instrument, includes the following steps: glycerol, deionized water, and a refractive index regulator are sequentially injected into a mixing tank via a water pump according to a preset ratio, and the stirring assembly is activated to fully mix the components; after full mixing, the viscosity of the solution in the mixing tank is detected online by a viscosity sensor, and the refractive index of the solution in the mixing tank is measured online by a refractive index detection module; after the central processing unit in the controller obtains the current viscosity detected in real time by the viscosity sensor, it automatically adjusts the addition ratio of glycerol and deionized water according to the detection result; simultaneously, after the central processing unit obtains the refractive index measured online by the refractive index detection module, it adjusts the amount of refractive index regulator according to the actual value until all parameters are within the target range;

[0012] After the stirring assembly stops working, an ultrasonic defoaming module is used to eliminate the gas generated during the mixing process. At the same time, a dissolved oxygen sensor measures the oxygen content dissolved in the solution in the mixing chamber in real time until the bubble content in the solution is reduced to the expected level. Then, the ultrasonic defoaming module is stopped. After defoaming the solution in the mixing chamber, the central processing unit acquires the current data detected by each sensor and adjusts the solution in the mixing chamber according to the current detection data. The above steps are repeated until all parameters are within the target range, thus completing the preparation of the bile-like working solution.

[0013] In one embodiment of the present invention, while the stirring assembly is started to fully mix the components, the temperature and density of the solution in the mixing tank are monitored in real time by an integrated temperature and density sensor. After the central processing unit obtains the temperature and density of the solution in the mixing tank monitored in real time by the integrated temperature and density sensor, it adjusts them to ensure that each indicator is within the target range.

[0014] In one embodiment of the present invention, while the stirring assembly is started to fully mix the components, the temperature and humidity in the laboratory are also monitored by an ambient temperature sensor and an ambient humidity sensor. After the central processing unit obtains the temperature and humidity in the laboratory monitored by the ambient temperature sensor and the ambient humidity sensor, it records the environmental changes in the laboratory and prompts whether manual or closed-loop fine-tuning is required based on the environmental changes in the laboratory to ensure the stability of the physical properties.

[0015] As described above, the apparatus and method for preparing a bile-like working solution according to the present invention have the following beneficial effects:

[0016] This invention utilizes a multi-channel automatic liquid injection system, or feeding mechanism, to precisely control the volume ratio of each component. A high-speed stirring and constant-temperature mixing chamber supports heating, cooling, stirring, and closed-environment mixing. Built-in viscosity sensors, a refractive index detection module, an integrated temperature and density sensor, and external ambient temperature and humidity sensors enable real-time monitoring of the solution's condition within the mixing chamber and changes in the laboratory environment. The central processing unit within the controller aggregates data from various sensors, performs real-time parameter comparison and algorithm correction, generates preparation reports, and stores batch data logs, ensuring batch traceability. This eliminates errors associated with traditional manual proportioning, guaranteeing high consistency in viscosity, refractive index, and density for each batch of liquid. It also prevents parameter deviations caused by factors such as temperature and humidity during preparation, achieving real-time correction through sensor feedback and automatic adjustment. This solves the problem of existing technologies lacking online monitoring of preparation process parameters (such as viscosity and refractive index), making it difficult to guarantee the consistency and repeatability of different batches of liquid. Attached Figure Description

[0017] Figure 1 This is a three-dimensional schematic diagram of the overall structure of the instrument for preparing the bile-like working fluid disclosed in this embodiment of the invention.

[0018] Figure 2 This is a front cross-sectional view of the mixing chamber in the bile-like working fluid preparation instrument disclosed in this embodiment of the invention.

[0019] Figure 3 This is a schematic diagram of the overall process of the preparation method of the bile-like working fluid disclosed in the embodiments of the present invention;

[0020] Figure 4 This is a diagram showing the information transmission relationship between the controller and various sensors and actuators in the bile-like working fluid preparation method disclosed in this embodiment of the invention.

[0021] Component designation explanation

[0022] 1. Base; 2. First drain hole; 3. Second drain hole; 4. Controller; 5. Feeding mechanism; 501. Feeding box; 502. First storage chamber; 503. Second storage chamber; 504. Third storage chamber; 505. Fourth storage chamber; 506. Cover plate; 507. Water pump; 508. First guide pipe; 509. Second guide pipe; 6. Mixing mechanism; 601. Mixing box; 602. Box cover; 603. First support frame; 604. Second support frame; 605. Protective shell; 606. First stirring rod; 607. Discharge pipe; 608. Second stirring rod; 7. Ambient temperature sensor; 8. Ambient humidity sensor; 9. Viscosity sensor; 10. Refractive index detection module; 11. Temperature and density integrated sensor; 12. Ultrasonic defoaming module; 13. Heating module. Detailed Implementation

[0023] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. It should be noted that, unless otherwise specified, the following embodiments and features described herein can be combined with each other.

[0024] The first embodiment of the present invention relates to an apparatus for preparing a bile-like working solution; please refer to [link to relevant documentation]. Figure 1 and Figure 2 The device includes a base 1, with several first drainage holes 2 connected to the interior of the base 1 on the top side, and several second drainage holes 3 connected to the interior of the base 1 on the front side. It should be noted that when using this preparation instrument to prepare the bile-like working solution, the waste liquid on the top side of the base 1 can be discharged through the first drainage holes 2 and the second drainage holes 3.

[0025] exist Figure 1 In the middle, the base 1 is provided with a feeding mechanism 5 and a mixing mechanism 6 located on the side of the feeding mechanism 5; wherein, the feeding mechanism 5 includes a feeding box 501, which is divided by a partition into a first storage chamber 502 for storing glycerin, a second storage chamber 503 for storing deionized water, and a third storage chamber 504 and a fourth storage chamber 505 for storing refractive index regulators. The top of the first storage chamber 502, the second storage chamber 503, the third storage chamber 504 and the fourth storage chamber 505 are all provided with a cover plate 506; the feeding mechanism 5 also includes a water pump 507, a first guide pipe 508 and a second guide pipe 509 installed on the side wall of the feeding box 501. One end of the first guide pipe 508 extends into the storage chamber, and the other end of the first guide pipe 508 passes through the cover plate 506 and is connected to the inlet of the water pump 507. One end of the second guide pipe 509 is connected to the outlet of the water pump 507, and the other end of the second guide pipe 509 extends into the mixing box 601;

[0026] It should be noted that the water pump 507 can inject various raw materials in the feeding box 501 into the mixing box 601 through the first guide pipe 508 and the second guide pipe 509. In practical applications, the injection method can also be to use a bottom one-way valve injection, that is, a one-way valve is installed at the bottom of the mixing box 601, and an injection port connected to each storage chamber is opened on the base 1. Therefore, it is only necessary to align the one-way valve on the mixing box 601 with the injection port and place it on the base 1. Under the action of water pressure or water pump, various raw materials in the feeding box 501 can also be injected into the mixing box 601.

[0027] exist Figure 1 and Figure 2In this embodiment, the mixing mechanism 6 includes a mixing tank 601 and a stirring assembly mounted on the base 1. The mixing tank 601 can be set to 500mL, 1L, and 1.5L depending on the actual capacity of the solution to be mixed. In practical applications, a fixing component for fixing the mixing tank 601 can be provided on the base 1 as needed. The inner wall of the mixing tank 601 is provided with a viscosity sensor 9, a refractive index detection module 10, a temperature and density integrated sensor 11, and a dissolved oxygen sensor that are uniformly distributed circumferentially. The inner bottom of the mixing tank 601 is provided with an ultrasonic defoaming module 12. The inner side of the mixing tank 601 is provided with a heating module 13. A cooling pipe may also be provided inside the inner side of the mixing tank 601.

[0028] It should be noted that the viscosity sensor 9 is used to detect the viscosity of the solution in the mixing tank 601 in real time, the refractive index detection module 10 is used to measure the refractive index of the solution in the mixing tank 601 in real time, the temperature and density integrated sensor 11 is used to monitor the temperature and density of the solution in the mixing tank 601 in real time, and the dissolved oxygen sensor is used to monitor the dissolved oxygen concentration in the solution in the mixing tank 601 in real time. In practical applications, heating is required when preparing bile-like working fluid, but when preparing other solutions, such as preparing a saturated sodium iodide solution, it will release heat. When preparing at an ambient temperature of 24 degrees Celsius, the solubility will increase due to its own exothermic reaction, so the coolant in the cooling pipe is needed to cool the solution in the mixing tank 601.

[0029] The mixing mechanism 6 also includes a cover 602 mounted on the mixing tank 601. The front side of the mixing tank 601 is also provided with a liquid outlet pipe 607. The stirring assembly includes a drive motor installed in the protective shell 605 and a first stirring rod 606 connected to the output shaft of the drive motor. The end of the first stirring rod 606 away from the drive motor extends through the protective shell 605 and the cover 602 into the mixing tank 601. The surface of the first stirring rod 606 is provided with a plurality of second stirring rods 608 located in the mixing tank 601. It should be noted that the drive motor can drive the first stirring rod 606 and the second stirring rods 608 to rotate, thereby stirring and mixing the solution in the mixing tank 601. Furthermore, there are a plurality of second stirring rods 608, and the plurality of second stirring rods 608 are arranged sequentially along the length of the first stirring rod 606, thereby making the liquid mix more uniform.

[0030] exist Figure 1In the middle, the base 1 is also provided with a support assembly movably connected to the protective shell 605. The support assembly includes a first support frame 603 installed on the base 1 and a second support frame 604 movably connected to the first support frame 603 through a first pin. The second support frame 604 is also movably connected to the protective shell 605 through a second pin. After the bile-like working fluid is prepared, the lid 602 on the mixing box 601 is removed, and then the second support frame 604 is pulled. Under the action of the first pin, the second support frame 604 rotates around the first pin. At the same time, the first stirring rod 606 is pulled, causing the first stirring rod 606 to rotate around the second pin. Thus, the stirring assembly can be taken out of the mixing box 601. In practical applications, a drive motor can also be installed on the first support frame 603 and the second support frame 604. The drive motor drives the second support frame 604 and the protective shell 605 to rotate, which can also remove the stirring assembly from the mixing box 601.

[0031] The base 1 is also equipped with a controller 4 that is connected to the viscosity sensor 9, the refractive index detection module 10, the temperature and density integrated sensor 11, the dissolved oxygen sensor, and the ultrasonic defoaming module 12. The first support frame 603 is equipped with an ambient temperature sensor 7 and an ambient humidity sensor 8. The drive motor, the ambient temperature sensor 7, and the ambient humidity sensor 8 are also connected to the controller 4. It should be noted that in this embodiment, the controller 4 can be used to control each sensor, or the controller 4 can be connected to a terminal computer device to control each sensor.

[0032] Specifically, the supporting preparation instrument includes the following modules: 1) A multi-channel automatic liquid injection system, also known as the feeding mechanism 5, which can accurately control the volume ratio of each component; 2) A high-speed stirring and constant temperature mixing chamber, which supports heating, stirring, and closed-environment mixing; 3) An online parameter detection module, which has the following built-in sensing systems: viscosity sensor 9 (rotational or capillary rheological); refractive index detection module 10 (optical critical angle or interferometric, with an accuracy of 5 significant digits); temperature and density integrated sensor 11; ambient temperature sensor 7; and ambient humidity sensor 8; 4) A central control and data acquisition system, based on an embedded controller 4 or a micro industrial computer, responsible for: issuing operation commands such as liquid injection, stirring, detection, and defoaming; summarizing data from each sensor, performing real-time parameter comparison and algorithm correction; generating preparation reports and storing batch data logs to ensure batch traceability; 5) An ultrasonic defoaming module 12, which eliminates bubbles generated during the mixing process through ultrasonic oscillation at a specific frequency; Through the above devices, key advantages such as accurate preparation, real-time monitoring, batch controllability, and strong experimental reproducibility of bile-like liquids can be achieved.

[0033] The second embodiment of the present invention relates to a method for preparing a bile-mimicking working fluid, specifically a method for preparing a bile-mimicking liquid for simulating biliary flow in PIV (particle imaging velocimetry) experiments. This liquid possesses adjustable refractive index, adjustable viscosity, temperature stability, and good fluorescent particle dispersibility, enabling it to realistically reproduce the flow characteristics of bile in an in vitro biomimetic biliary model. It is widely used in fluid dynamics visualization experiments such as biliary disease research, biliary stent performance evaluation, and biliary drug delivery behavior simulation. The process is as follows: Figure 3 As shown, the details are as follows:

[0034] Step 101: Glycerin, deionized water and refractive index modifier are injected into the mixing tank 601 in sequence by water pump 507 according to a preset ratio, and the stirring assembly is started to fully mix the components.

[0035] Step 102: After thorough mixing, the viscosity of the solution in the mixing tank 601 is detected online by the viscosity sensor 9, and the refractive index of the solution in the mixing tank 601 is measured online by the refractive index detection module 10.

[0036] Step 103: After the central processing unit in the controller 4 obtains the current viscosity detected in real time by the viscosity sensor 9, it automatically adjusts the addition ratio of glycerol and deionized water according to the detection result. At the same time, after the central processing unit obtains the refractive index measured online by the refractive index detection module 10, it adjusts the amount of refractive index regulator according to the actual value until all parameters are within the target range.

[0037] Specifically, adjusting the ratio of glycerol to deionized water involves measuring the refractive index and viscosity during solution preparation to determine if the solution is up to standard. If it is not up to standard, such as if the refractive index is too low, an appropriate amount of glycerol needs to be added; if it is too high, an appropriate amount of deionized water needs to be added, about 5 ml each time. After stirring, the solution is measured again to see if it meets the standard, until all parameters are within the target range.

[0038] Step 104: After the stirring assembly stops working, the ultrasonic defoaming module 12 is used to eliminate the gas generated during the mixing process. At the same time, the dissolved oxygen sensor measures the oxygen content dissolved in the solution in the mixing chamber 601 in real time until the bubble content in the solution is reduced to the expected level, then the ultrasonic defoaming module 12 is stopped.

[0039] Step 105: After defoaming the solution in the mixing tank 601, the central processing unit acquires the current data detected by each sensor and adjusts the solution in the mixing tank 601 according to the current data. The above steps are repeated until all parameters are within the target range, thus completing the preparation of the bile-like working solution.

[0040] Furthermore, during the activation of the stirring assembly to fully mix the components, the temperature and density of the solution within the mixing chamber 601 are monitored in real time by an integrated temperature and density sensor 11. The central processing unit acquires the real-time temperature and density data from the integrated temperature and density sensor 11 and adjusts it to ensure that all indicators are within the target range. Additionally, the temperature and humidity within the laboratory are monitored by a temperature sensor 7 and a humidity sensor 8. After acquiring the temperature and humidity data from the ambient temperature sensor 7 and the ambient humidity sensor 8, the central processing unit records changes in the laboratory environment and indicates whether manual or closed-loop fine-tuning is needed based on these changes to ensure material stability. For the information transmission relationship between the controller and the various sensors and actuators, please refer to [link to relevant documentation]. Figure 4 .

[0041] Furthermore, 1) Basic system selection: This invention uses glycerol-water as the basic system, supplemented with refractive index regulators (such as saturated sodium iodide solution, anhydrous sodium thiosulfate particles), etc. The dynamic viscosity can be adjusted within the range of 2-6 mPa·s (room temperature 21℃) by adjusting the mass ratio of glycerol to deionized water; the refractive index is adjustable within the range of 1.4-1.42, matching the refractive index of commonly used model silica gel; 2) Multi-parameter detection and adjustment process: Glycerol, deionized water, and refractive index regulator are injected into the mixing chamber in a preset ratio; the stirring device is started to fully mix the components; the viscosity sensor 9 (such as a rotational rheology module) is used to detect the current viscosity online, and the addition ratio of glycerol and water is automatically adjusted according to the detection results; the refractive index is measured online through the refractive index detection module 10, and the amount of refractive index regulator is adjusted according to the actual value; the temperature and density of the current solution are monitored in real time through the temperature and density integrated sensor 11 to ensure that each index is within the target range; the ambient temperature sensor 7 and the ambient humidity sensor 8 are used to record changes in the laboratory environment and indicate whether manual or closed-loop fine-tuning is required to ensure the stability of physical properties;

[0042] 3) Ultrasonic Defoaming: The ultrasonic defoaming module 12 uses ultrasonic oscillation to break and release tiny bubbles in the solution, reducing the bubble content to an extremely low level and eliminating PIV measurement artifact interference; 4) Final Performance Calibration: After defoaming, the solution in the mixing tank 601 undergoes a rapid online test (viscosity, refractive index, density, temperature) to confirm that all indicators match the preset values ​​before it can be output for use; if any parameter exceeds the allowable error range (±5%), the system will automatically prompt for readjustment by adding relevant raw materials or secondary defoaming.

[0043] In summary, this invention aims to solve the problem of precise control and standardized preparation of bile-like liquids in in vitro bile duct fluid visualization experiments, achieving standardized and batch preparation of bile-like liquids. This invention eliminates errors from traditional manual proportioning through automatic liquid injection and online detection, ensuring high consistency in viscosity, refractive index, and density for each batch of liquid. This invention provides real-time online property monitoring and closed-loop correction functions to avoid parameter deviations caused by factors such as temperature and environmental humidity during preparation. Real-time correction is achieved through sensor feedback and automatic adjustment. This invention can eliminate bubble interference, improving the accuracy and image quality of PIV experiments. It employs ultrasonic debubbling technology to minimize the content of microbubbles, avoiding refractive interference or light spot artifacts caused by bubbles in PIV imaging.

[0044] This invention simplifies the experimental process and improves reproducibility. The entire preparation process is automated through a central control system, shortening the preparation time. Batch data is automatically recorded, facilitating the comparison and verification of experimental results. This invention provides a highly reliable foundation for biliary hydrodynamics research, stent testing, and preclinical trials. Through precise and controllable experimental media, more realistic and repeatable flow field data can be obtained in hydrodynamic simulations, providing a precise and consistent physical property basis for subsequent biliary stent design optimization and drug delivery efficiency evaluation, thereby improving research and verification efficiency.

[0045] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. All equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this invention should still be covered by the claims of this invention.

Claims

1. An instrument for preparing a bile-like working solution, comprising a base (1), characterized in that: The base (1) is provided with a feeding mechanism (5) and a mixing mechanism (6) located on the side of the feeding mechanism (5); The feeding mechanism (5) includes a feeding box (501), which is divided by a partition into a first storage chamber (502) for storing glycerin, a second storage chamber (503) for storing deionized water, and a third storage chamber (504) and a fourth storage chamber (505) for storing refractive index regulators. The mixing mechanism (6) includes a mixing chamber (601) and a stirring assembly disposed on the base (1). The inner wall of the mixing chamber (601) is provided with a viscosity sensor (9), a refractive index detection module (10), a temperature and density integrated sensor (11), and a dissolved oxygen sensor that are uniformly distributed in the circumferential direction. The bottom of the mixing chamber (601) is provided with an ultrasonic defoaming module (12). The base (1) is also equipped with a controller (4) that is connected to the viscosity sensor (9), the refractive index detection module (10), the temperature and density integrated sensor (11), the dissolved oxygen sensor, and the ultrasonic defoaming module (12), respectively.

2. The instrument for preparing a bile-like working solution according to claim 1, characterized in that: The top side of the base (1) is provided with several first drainage holes (2) that communicate with the interior of the base (1), and the front side of the base (1) is also provided with several second drainage holes (3) that communicate with the interior of the base (1).

3. The apparatus for preparing a bile-like working solution according to claim 1, characterized in that: The top of the first storage chamber (502), the second storage chamber (503), the third storage chamber (504) and the fourth storage chamber (505) are all provided with a cover plate (506). The feeding mechanism (5) also includes a water pump (507), a first guide pipe (508) and a second guide pipe (509) installed on the side wall of the feeding box (501). One end of the first guide pipe (508) extends into the storage chamber, and the other end of the first guide pipe (508) passes through the cover plate (506) and is connected to the inlet of the water pump (507). One end of the second guide pipe (509) is connected to the outlet of the water pump (507), and the other end of the second guide pipe (509) extends into the mixing box (601).

4. The apparatus for preparing a bile-like working solution according to claim 1, characterized in that: The mixing mechanism (6) also includes a cover (602) mounted on the mixing tank (601). The stirring assembly includes a drive motor mounted in a protective shell (605) and a first stirring rod (606) connected to the output shaft of the drive motor. The end of the first stirring rod (606) away from the drive motor extends through the protective shell (605) and the cover (602) into the mixing tank (601). The surface of the first stirring rod (606) is provided with a plurality of second stirring rods (608) located in the mixing tank (601).

5. The apparatus for preparing a bile-like working solution according to claim 4, characterized in that: The base (1) is also provided with a support assembly that is movably connected to the protective shell (605). The support assembly includes a first support frame (603) installed on the base (1) and a second support frame (604) movably connected to the first support frame (603). The second support frame (604) is also movably connected to the protective shell (605). An ambient temperature sensor (7) and an ambient humidity sensor (8) are provided on the first support frame (603). The drive motor, the ambient temperature sensor (7), and the ambient humidity sensor (8) are also connected to the controller (4).

6. A method for preparing a bile-like working fluid, characterized in that: The apparatus for preparing the bile-like working solution according to any one of claims 1 to 5 includes the following steps: Glycerin, deionized water and refractive index modifier are injected into the mixing tank (601) in sequence by water pump (507) according to a preset ratio, and the stirring assembly is started to fully mix the components; After thorough mixing, the viscosity of the solution in the mixing tank (601) is detected online by a viscosity sensor (9), and the refractive index of the solution in the mixing tank (601) is measured online by a refractive index detection module (10). After the central processing unit in the controller (4) obtains the current viscosity detected in real time by the viscosity sensor (9), it automatically adjusts the addition ratio of glycerol and deionized water according to the detection result. At the same time, after the central processing unit obtains the refractive index measured online by the refractive index detection module (10), it adjusts the amount of refractive index regulator according to the actual value until all parameters are within the target range. After the stirring assembly stops working, the ultrasonic defoaming module (12) is used to eliminate the gas generated during the mixing process. At the same time, the dissolved oxygen sensor measures the oxygen content dissolved in the solution in the mixing tank (601) in real time until the bubble content in the solution is reduced to the expected level, then the ultrasonic defoaming module (12) is stopped. After defoaming the solution in the mixing tank (601), the central processing unit acquires the current data detected by each sensor and adjusts the solution in the mixing tank (601) according to the current data. The above steps are repeated until all parameters are within the target range, thus completing the preparation of the bile-like working solution.

7. The method for preparing a bile-like working solution according to claim 6, characterized in that: During the start-up of the stirring assembly to fully mix the components, the temperature and density of the solution in the mixing tank (601) are monitored in real time by an integrated temperature and density sensor (11). After the central processing unit obtains the temperature and density of the solution in the mixing tank (601) monitored in real time by the integrated temperature and density sensor (11), it adjusts them to ensure that each indicator is within the target range.

8. The method for preparing a bile-like working solution according to claim 6, characterized in that: While the stirring assembly is started to fully mix the components, the temperature and humidity in the laboratory are monitored by the ambient temperature sensor (7) and the ambient humidity sensor (8). After the central processing unit obtains the temperature and humidity in the laboratory monitored by the ambient temperature sensor (7) and the ambient humidity sensor (8), it records the environmental changes in the laboratory and prompts whether manual or closed-loop fine-tuning is needed based on the environmental changes in the laboratory to ensure the stability of the physical properties.

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