Ginkgo biflavone-containing skin anti-wrinkle whitening composition and preparation method thereof
By using ginkgo biflavonoids to formulate an injectable preparation, the irritation and safety issues of existing anti-wrinkle and whitening products have been resolved, achieving significant anti-wrinkle and whitening effects on the skin, and improving skin elasticity and pigmentation.
Patent Information
- Application Number
- CN202511318945.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-16
- Publication Date
- 2025-11-25
AI Technical Summary
Existing anti-wrinkle and whitening products have issues with irritation and safety, and their effects are limited, making it difficult to fundamentally solve the problems of skin aging and pigmentation.
Using Ginkgo biloba flavonoids as the active ingredient, combined with polyethylene glycol 300 and PBS buffer, an injectable formulation was prepared. The stability and safety of the product were ensured through optimized preparation process, allowing it to act directly on deep skin tissues.
Ginkgo biloba extract significantly improves skin elasticity, reduces wrinkles, has good antioxidant capacity and whitening effect, and is non-irritating and stable to the skin.
Smart Images
Figure CN121003561A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to a skin anti-wrinkle and whitening product containing ginkgo biflavonoids and its preparation method, belonging to the field of cosmetic technology. Background Technology
[0002] As people place increasing importance on skin care, the development of anti-wrinkle and whitening products and cosmetics is also accelerating. However, existing anti-wrinkle and whitening products still have many problems.
[0003] Irritation: Many traditional anti-wrinkle and whitening ingredients, such as retinol and retinoic acid, while having some effect on improving wrinkles or increasing skin elasticity, can irritate the skin even in small amounts. Furthermore, their poor stability and susceptibility to oxidation and denaturation in air limit their application in anti-wrinkle and whitening products.
[0004] Safety: Some chemically synthesized anti-wrinkle and whitening ingredients may have potential side effects, and long-term use may have adverse effects on skin health.
[0005] Limited effectiveness: While some anti-wrinkle and whitening products can temporarily improve the appearance of the skin, their effects on the production of collagen and elastin in the deeper layers of the skin and the enhancement of antioxidant capacity are limited, making it difficult to fundamentally solve the problems of skin aging and pigmentation.
[0006] The aforementioned problems not only cause suffering for patients and affect their quality of life, but also place a burden on the healthcare system. Therefore, finding an efficient and convenient method for skin anti-wrinkle and whitening is a critical clinical issue that urgently needs to be addressed.
[0007] Ginkgetin is a natural flavonoid product derived from the leaves and seeds of the ginkgo tree (Ginkgo biloba). Current research has confirmed the anti-inflammatory and antioxidant effects of ginkgetin in COPD, cardiovascular diseases, and neurological disorders, demonstrating its potential and application prospects in the treatment of various diseases. However, there are no reports on the correlation between ginkgetin and skin anti-wrinkle and whitening effects. Summary of the Invention
[0008] The purpose of this invention is to fill the gap in the application of ginkgo biflavonoids in skin anti-wrinkle and whitening.
[0009] To achieve the above objectives, a first aspect of the present invention provides a skin anti-wrinkle and whitening composition, wherein the active ingredient in the composition includes ginkgo biflavonoids.
[0010] In some embodiments, the concentration of ginkgo biloba flavonoids in the composition is 5 mg / ml.
[0011] In some embodiments, the composition is an injectable formulation.
[0012] In some embodiments, the excipients in the composition include polyethylene glycol 300 and PBS buffer, wherein the volume ratio of polyethylene glycol 300 to PBS buffer is 1:1.
[0013] A second aspect of the present invention provides a method for preparing the above-mentioned anti-wrinkle and whitening skin composition, comprising the following steps:
[0014] Polyethylene glycol 300 was thoroughly mixed with PBS buffer to obtain a mixture.
[0015] Add ginkgo biloba flavonoids to the above mixture and stir thoroughly at room temperature until the ginkgo biloba flavonoids are completely dissolved to obtain a ginkgo biloba flavonoid solution.
[0016] After sterilization, the Ginkgo biloba flavonoid solution yields a skin anti-wrinkle and whitening composition.
[0017] In some embodiments, the volume ratio of polyethylene glycol 300 to PBS buffer is 1:1.
[0018] In some embodiments, the skin anti-wrinkle and whitening composition is used immediately after preparation.
[0019] In some embodiments, after the skin anti-wrinkle and whitening composition is prepared, it is dispensed into sterile containers and frozen at -80°C or lower in the dark.
[0020] In some embodiments, the skin anti-wrinkle and whitening composition may be frozen for no more than 6 months.
[0021] In some embodiments, the frozen skin anti-wrinkle and whitening composition is removed, thawed in a water bath at a temperature not exceeding 37°C, and then shaken to mix before use.
[0022] The beneficial effects of this invention are as follows: By using naturally derived ginkgo biflavonoids as the active ingredient, a novel anti-wrinkle and whitening skin product is formulated, which has significant therapeutic effects on skin wrinkle reduction and whitening. At the same time, it solves the problems of irritation and safety issues existing in current anti-wrinkle and whitening products. Attached Figure Description
[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings involved in the embodiments will be briefly described below.
[0024] Figure 1 These are gross photographs of the skin pigmentation status of the experimental mice in each group in Example 3.
[0025] Figure 2 These are photographs taken under a dermoscope of the skin pigmentation status of the experimental mice in each group in Example 3.
[0026] Figure 3 The results of HE staining of skin specimens from each group of experimental mice in Example 3 are shown.
[0027] Figure 4 The results of Masson-Fontana staining of skin specimens from each group of experimental mice in Example 3 are shown. Detailed Implementation
[0028] This patent provides a skin anti-wrinkle and whitening composition containing ginkgo biloba extract, aiming to offer a safe, effective, and stable solution for skin anti-wrinkle and whitening, meeting consumers' demand for high-quality skin care products. This patent fills a gap in the application of ginkgo biloba extract for skin anti-wrinkle and whitening.
[0029] The active ingredient in the anti-wrinkle and whitening skin composition provided in this patent is ginkgo biflavonoids, whose molecular formula is C0.05. 32 H 22 O 10 There are no specific restrictions on the source of ginkgo biflavonoids; they can be obtained through self-extraction or purchased commercially.
[0030] The concentration of ginkgo biloba flavonoids in the skin anti-wrinkle and whitening composition is 5 mg / ml. Extensive experimental studies have shown that at this concentration, ginkgo biloba flavonoids can maximize their anti-wrinkle and whitening effects while ensuring safety.
[0031] Preferably, the skin anti-wrinkle and whitening composition is formulated into an injectable preparation, which enables the active ingredients to act more directly on the deep skin tissues, improves the utilization rate of the ingredients, and thus enhances the anti-wrinkle and whitening effect.
[0032] The excipients in the skin anti-wrinkle and whitening composition include polyethylene glycol 300 and PBS buffer, with a volume ratio of 1:1. This synergistic effect ensures the stability and effectiveness of the composition. Polyethylene glycol 300 has good solubility, which helps Ginkgo biloba extract dissolve better. PBS buffer (Phosphate-Buffered Saline) is one of the most commonly used buffer solutions in biomedicine, molecular biology, and cell biology. Its core function is to maintain pH stability and provide osmotic pressure and ion concentration similar to the biological environment (such as body fluids). In this patent, the role of PBS buffer is to maintain the pH stability of the skin anti-wrinkle and whitening composition, ensuring that Ginkgo biloba extract remains active in a suitable environment, and also promoting the absorption of the active ingredient of Ginkgo biloba extract in the injectable formulation by the skin.
[0033] The preparation method of the above-mentioned skin anti-wrinkle and whitening composition containing ginkgo biloba flavonoids includes the following steps:
[0034] First, polyethylene glycol 300 and PBS buffer are thoroughly mixed to obtain a mixture. This step ensures that the two excipients are uniformly mixed, providing a good solvent environment for the subsequent dissolution of ginkgo biloba extract.
[0035] Then, add ginkgo biloba flavonoids to the above mixture and stir thoroughly at room temperature until the ginkgo biloba flavonoids are completely dissolved to obtain a ginkgo biloba flavonoid solution. Room temperature is favorable for operation and will not affect the activity of ginkgo biloba flavonoids due to excessively high or low temperatures. Thorough stirring ensures that ginkgo biloba flavonoids are evenly dispersed in the mixture, forming a homogeneous and stable solution. After necessary sterilization, the ginkgo biloba flavonoid solution yields the skin anti-wrinkle and whitening composition.
[0036] Once the ginkgo biloba flavonoid solution is prepared, there are two methods for use and storage. One is to use it immediately, which maximizes the freshness and activity of the composition. The other method is to dispense the prepared ginkgo biloba flavonoid solution into sterile containers and freeze it at -80°C or lower, protected from light. Low temperature and light protection effectively slow down the degradation rate of ginkgo biloba flavonoids, maintaining their activity.
[0037] Frozen ginkgo biloba extract solution should not be stored for more than 6 months to ensure its anti-wrinkle and whitening effects are not significantly affected within its shelf life. When needed, remove the frozen ginkgo biloba extract solution and thaw it to room temperature in a water bath at a temperature not exceeding 37°C. After thawing, shake the solution well and use immediately. A water bath temperature not exceeding 37°C avoids reducing the activity of ginkgo biloba extract due to excessive heat, shaking well after thawing ensures the homogeneity of the solution, and immediate use ensures the product's effectiveness.
[0038] The anti-wrinkle and whitening skin composition of the present invention has the following advantages:
[0039] High safety profile: Ginkgo biloba extract is derived from natural plants, is non-irritating to the skin, and is safe and reliable to use.
[0040] Significant effects: Ginkgo biloba extract can effectively inhibit the activity of collagenase and elastase, and promote the synthesis of collagen and elastin, thereby significantly improving skin elasticity and reducing wrinkles. At the same time, its antioxidant capacity and pigmentation-regulating effects can also effectively whiten the skin.
[0041] Good stability: By optimizing the formulation and preparation process of the composition, the prepared composition has good stability, is not easily oxidized or denatured, and can maintain its anti-wrinkle and whitening effects for a long time.
[0042] The technical solutions provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0043] Example 1
[0044] Preparation of anti-wrinkle and whitening skin composition
[0045] Prepare the raw materials: Accurately measure appropriate amounts of polyethylene glycol 300, PBS buffer, and ginkgo biloba extract. For example, measure 5 ml of polyethylene glycol 300 and 5 ml of PBS buffer in a 1:1 volume ratio, and weigh 50 mg of ginkgo biloba extract to prepare 10 ml of ginkgo biloba extract solution with a concentration of 5 mg / ml.
[0046] Mixing excipients: Slowly pour the measured polyethylene glycol 300 into PBS buffer while stirring thoroughly with a magnetic stirrer or glass rod at a speed of 200-300 rpm for 5-10 minutes until the two excipients are completely mixed and a clear, transparent mixture is obtained.
[0047] Dissolving Ginkgo Biloba Flavonoids: Gradually add the weighed ginkgo biloba flavonoids to the above mixture and continue stirring at room temperature (20-25℃). The stirring speed can be appropriately increased to 300-400 rpm, and the stirring time is about 15-20 minutes, until the ginkgo biloba flavonoids are completely dissolved and the solution becomes clear and transparent again, thus obtaining a ginkgo biloba flavonoid solution. After sterilizing the solution, the skin anti-wrinkle and whitening composition is obtained.
[0048] Example 2
[0049] Use and storage of anti-wrinkle and whitening skin composition
[0050] Immediate Use: Once prepared, the composition can be directly injected. For example, in a professional medical aesthetic institution, a qualified medical professional can inject an appropriate amount of the composition into the areas of skin that need improvement, following standard operating procedures. The injection dosage and injection site depend on the patient's specific skin condition and needs, generally ranging from 0.5 to 2 ml per injection, primarily focusing on areas of the face with wrinkles or dullness.
[0051] Cryopreservation: If the composition is not to be used immediately after preparation, aliquot it into sterile glass ampoules or plastic cryovials. The amount dispensed into each ampoule (or tube) should be determined according to actual needs, such as 1–5 ml. After aliquoting, quickly place the ampoule into an ultra-low temperature freezer at -80°C or a liquid nitrogen tank at an even lower temperature, protecting it from light. Throughout the cryopreservation process, strictly record the cryopreservation time to ensure it does not exceed 6 months.
[0052] Thawing and Use: When needed, remove the frozen ginkgo biloba extract solution from the freezer or liquid nitrogen tank and place it in a constant temperature water bath at 35–37°C to allow the solution to gradually thaw to room temperature. During thawing, gently shake the container every 2–3 minutes to ensure even heating. After thawing, shake again to mix thoroughly, and then immediately inject according to standard operating procedures.
[0053] Example 3
[0054] To verify the effect of ginkgo biloba flavonoids on pigmentation in mice with melasma.
[0055] 1. Animals
[0056] Animal source: C57 mice, female, 8 weeks old, weighing 22-24g, were used in animal experiments and purchased from Shanghai Paijieluo Biotechnology Co., Ltd. All mice were treated with SPF-grade 12h light / 12h dark at a standard temperature of 23±2℃, and the mice had free access to high-fat or standard diets and water.
[0057] Animal model: Five C57 mice were used as the normal control group, and another 15 C57 mice were randomly divided into three groups: the model group, the ginkgo biflavonoid treatment group, and the tranexamic acid positive treatment group, with five mice in each group. The hair on the backs of all mice was shaved off, with a shaved area of approximately 4×4 cm.
[0058] Before establishing the melasma model, mice were anesthetized with isoflurane, and then progesterone was injected alternately into the groin muscles of the mice at a dose of 25 mg / kg / day. This meant that progesterone was injected daily at a dose of 25 mg / kg based on the mouse's body weight for 21 consecutive days. Each mouse weighed 22–24 g, and the daily progesterone injection was approximately 0.6 mg.
[0059] Mice were injected with progesterone and then subjected to UV irradiation at an intensity of 4 mW / cm². 2 Irradiate 5 days a week. In the first week, each irradiation session lasts 2 minutes; in the second week, each irradiation session lasts 3 minutes; and in the third week, each irradiation session lasts 4 minutes.
[0060] Ginkgo biloba extract was administered at a dose of 50 mg / kg / day, calculated based on the body weight of each mouse. Starting from day 14, mice were injected with ginkgo biloba extract to treat melasma. Ginkgo biloba extract was injected intraperitoneally into the mice daily at a dose of 50 mg / kg for a total of 14 days.
[0061] All mice were shaved once a week, and photographed and sampled after 3 weeks.
[0062] 2. Grouping and Treatment
[0063] (1) Normal control group: The base solvent of the skin anti-wrinkle and whitening composition product was used to inject mice intraperitoneally at a dosage of 0.2 ml / mouse / day for 21 consecutive days.
[0064] The base solvent described above consists of the following components at the following volume concentrations: 50% polyethylene glycol 300 (PEG300) + 50% PBS buffer. This is consistent with the solvent composition in the skin anti-wrinkle and whitening product prepared in this patent. The base solvent does not contain ginkgo biloba extract, the active ingredient.
[0065] (2) Melasma group: Melasma model was created in the same manner as the normal control group. The mice were injected intraperitoneally with the same base solvent as the normal control group at a dose of 0.2 ml / mouse / day for 21 consecutive days.
[0066] (3) Ginkgo biloba flavonoid treatment group: Ginkgo biloba flavonoid monomer was dissolved in the product base solvent at a concentration of 5 mg / ml and injected intraperitoneally into mice at a dosage of 0.2 ml / mouse / day. Treatment started on day 14 and continued for 14 days.
[0067] (4) Tranexamic acid positive control treatment group: Tranexamic acid injection was administered to mice via intraperitoneal injection at a dose of 0.2 ml / mouse / day, starting from day 14 and continuing for 14 days.
[0068] 3. Experimental Methods
[0069] (1) Skin pigmentation analysis
[0070] Macroscopic observation: Gross photographs were taken on days 7 and 14 after drug administration. Some photos are shown below. Figure 1 As shown. Photos were taken under a dermoscope for observation; some photos are shown below. Figure 2 As shown in the two photographs above, the skin pigmentation of the mice was observed.
[0071] (2) Histological hematoxylin-eosin staining (HE staining)
[0072] Mice were sacrificed two weeks after treatment, and skin specimens were fixed in 4% neutral formaldehyde buffer for 24 hours. Routine histological examination was performed, including paraffin embedding, sectioning, and HE staining. Sections were observed under a digital slide scanner; the slide images are shown below. Figure 3 As shown.
[0073] (3) Masson-Fontana staining (melanin staining)
[0074] Mice were sacrificed two weeks after treatment, and skin specimens were fixed in 4% neutral formaldehyde buffer for 24 hours. Routine histological examination was performed, including paraffin embedding, sectioning, and Masson-Fontana staining. Sections were observed under a digital slide scanner; the slide images are shown below. Figure 4 As shown.
[0075] 4. Experimental Results
[0076] The effects of ginkgo biloba flavonoids on skin pigmentation in vivo were observed using gross photography, dermoscopy, HE staining, and Masson-Fontana staining. The results are shown in the table below. Figures 1 to 4 As shown. From Figure 1 and Figure 2 The comparison of photos from each group showed that, compared with the melasma model group, the Ginkgo biloba flavonoid treatment group had a more significant wound healing effect after 1 and 2 weeks of treatment, and the effect was better than that of the tranexamic acid positive control treatment group.
[0077] according to Figure 4 Melanin staining results showed that after two weeks, the melanin granules in the ginkgo biloba flavonoid treatment group were significantly reduced compared to the melasma model group, and the effect was superior to that in the tranexamic acid positive control group. This result indicates that melanin pigmentation on the back skin of melasma mice is reduced, and ginkgo biloba flavonoid intervention can effectively improve skin pigmentation and has a whitening effect on the skin, with a more significant effect than tranexamic acid.
[0078] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.
Claims
1. A skin anti-wrinkle and whitening composition, characterized in that, The active ingredient in the composition includes ginkgo biflavonoids.
2. The skin anti-wrinkle and whitening composition according to claim 1, characterized in that, The concentration of ginkgo biflavonoids in the composition is 5 mg / ml.
3. The skin anti-wrinkle and whitening composition according to claim 1, characterized in that, The composition is an injectable formulation.
4. The skin anti-wrinkle and whitening composition according to claim 1, characterized in that, The excipients in the composition include polyethylene glycol 300 and PBS buffer, wherein the volume ratio of polyethylene glycol 300 to PBS buffer is 1:
1.
5. The method for preparing the skin anti-wrinkle and whitening composition according to claim 1, characterized in that, Includes the following steps: Polyethylene glycol 300 was thoroughly mixed with PBS buffer to obtain a mixture. Ginkgo biloba flavonoids were added to the above mixture and stirred thoroughly at room temperature until the ginkgo biloba flavonoids were completely dissolved to obtain a ginkgo biloba flavonoid solution. The ginkgo biflavonoid solution is sterilized to obtain the skin anti-wrinkle and whitening composition.
6. The method for preparing the skin anti-wrinkle and whitening composition according to claim 5, characterized in that, The volume ratio of polyethylene glycol 300 to the PBS buffer is 1:
1.
7. The method for preparing the skin anti-wrinkle and whitening composition according to claim 5, characterized in that, The skin anti-wrinkle and whitening composition is used immediately after preparation.
8. The method for preparing the skin anti-wrinkle and whitening composition according to claim 5, characterized in that, After the skin anti-wrinkle and whitening composition is prepared, it is dispensed into sterile containers and stored in the dark at a temperature of -80°C or lower.
9. The method for preparing the skin anti-wrinkle and whitening composition according to claim 8, characterized in that, The skin anti-wrinkle and whitening composition shall be frozen for no more than 6 months.
10. The method for preparing the skin anti-wrinkle and whitening composition according to claim 8, characterized in that, Remove the frozen skin anti-wrinkle and whitening composition, thaw it in a water bath at a temperature not exceeding 37°C, shake the thawed skin anti-wrinkle and whitening composition to mix well, and then use it immediately.
Citation Information
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