Seed treatment suspending agent containing clothianidin, prothioconazole and fludioxonil

Abstract

The invention relates to the technical field of seed treatment suspending agents. The invention discloses a seed treatment suspending agent containing clothianidin, prothioconazole and fludioxonil as well as a preparation method and application of the seed treatment suspending agent. The seed treatment suspending agent is prepared from the following raw materials: clothianidin, prothioconazole, fludioxonil, a bactericidal synergist, a dispersing agent, a wetting dispersing agent, a thickening agent, a preservative, an anti-freezing agent, a film-forming agent, warning coloration, a de-foaming agent and de-ionized water. The seed treatment suspending agent containing clothianidin, prothioconazole and fludioxonil prepared by the invention is good in suspending performance, low-temperature stability and heat storage stability. The application amount and the harm to the environment are reduced. The invention also relates to application of the seed treatment suspending agent in prevention and treatment of diseases and insect pests.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticide insecticidal and fungicidal, and particularly relates to a use of a seed treatment suspension agent containing clothianidin, prothioconazole and fludioxonil for preventing and treating wheat diseases and insect pests. BACKGROUND

[0002] Current seed treatment agents are faced with the dual threat of soil-borne diseases (such as wheat foot rot, corn silk smut, and wheat root rot) and pests (grubs, needle bugs, and aphids), and single-component prevention and control has defects such as narrow spectrum and short duration. Traditional organophosphorus agents (such as phoxim) are gradually being eliminated due to resistance development and environmental risks, and there is an urgent need to develop efficient and low-toxicity multi-target compound systems.

[0003] Clothianidin belongs to the neonicotinoid insecticide, and its mechanism of action is to selectively activate the insect nicotinic acetylcholine receptor (nAChR) to block nerve conduction and cause pests to paralyze and die. The agent has contact, stomach and strong systemic triple action modes, and shows significant prevention and control effect (field prevention and control effect > 90%) on hemiptera (aphids, planthoppers, leafhoppers), coleoptera (scarab beetle larvae), diptera (thrips) and some lepidoptera pests. Compared with traditional organophosphorus pesticides, clothianidin has outstanding advantages such as broad spectrum, high efficiency, low toxicity, long duration, and no cross-resistance with existing insecticides, and has become a core variety for replacing high-toxicity pesticides in rice seed soaking, vegetable spraying and fruit tree root irrigation scenes, and is listed by the European Union as a key chemical pesticide reduction and substitution product.

[0004] Prothioconazole is a new type of broad-spectrum triazole sulfone fungicide developed by Bayer Company, mainly used for preventing and treating many diseases of crops such as cereals, wheat, beans, etc. Prothioconazole is low in toxicity, non-teratogenic and non-mutagenic, safe to humans and the environment. Its mechanism of action is to inhibit the demethylation of lanosterol or 2,4-methylene dihydro lanosterol at position 14, which is a precursor of fungal sterols. The prevention and control effect on foot rot caused by fusarium is 85%-95%, and it also has systemic conductive properties.

[0005] Fludioxonil is a new type of pyrrole non-systemic broad-spectrum fungicide, which can significantly prevent and control seed-borne / soil-borne diseases (such as damping-off) by interfering with the glucose phosphorylation of mycelium to block energy metabolism, and has no cross-resistance with existing agents. Irrigation or soil treatment has very good effect on many root diseases such as damping-off, root rot, wilt, and blight of various crops. In addition, fludioxonil can also be used for spraying to prevent and control gray mold and sclerotinia of various crops. It is very safe to crop seeds, does not affect seed emergence, and can promote early seed emergence, uniform emergence, and strong growth.

[0006] CN117099795A discloses a seed treatment suspension containing prothioconazole, fludioxonil and thiamethoxam for preventing and treating wheat foot rot and improving wheat growth activity.

[0007] CN116849222A discloses a pesticide composition comprising active ingredient A and active ingredient B, wherein the active ingredient A is prothioconazole, the active ingredient B is fludioxonil, and the pesticide composition further comprises thiamethoxam. The seed treatment suspension can prevent and treat wheat root rot, and has a synergistic effect after compounding, thereby reducing the amount of pesticide used.

[0008] CN110432277A discloses a seed treatment suspension with effective components of prothioconazole, fludioxonil and thiamethoxam. After the seed treatment suspension is mixed with water and rice seeds, the germination rate of the rice seeds can be significantly improved, the occurrence of crop diseases and pests at the seedling stage can be reduced, and the yield of crops can be increased.

[0009] Although the above-mentioned patents disclose seed treatment suspensions containing thiamethoxam, prothioconazole and fludioxonil, the properties of the seed treatment suspensions are unstable and should be stratified and deteriorated. SUMMARY

[0010] Based on the above, the present application aims to provide a seed treatment suspension containing thiamethoxam, prothioconazole and fludioxonil for preventing and treating wheat diseases and pests and improving wheat growth activity, and the seed treatment suspension has good suspending performance, cold and hot storage stability. The present application provides a seed treatment suspension containing thiamethoxam, prothioconazole and fludioxonil, a preparation method and an application thereof. Another object of the present application is to apply the seed treatment suspension containing thiamethoxam, prothioconazole and fludioxonil to prevent and treat wheat root rot and wheat aphids.

[0011] In one aspect, the present application provides a seed treatment suspension containing thiamethoxam, prothioconazole and fludioxonil, wherein the weight ratio of thiamethoxam, prothioconazole and fludioxonil is 10-40:1-15:1-10. In a preferred embodiment, the weight ratio of thiamethoxam, prothioconazole and fludioxonil is 20-30:1-10:1-5. To make the effect more obvious, the weight ratio of the three can be further preferably 22-28:1-5:1-3.

[0012] The raw material composition includes, by mass percentage, 10-40% of thiamethoxam technical material, 1-15% of prothioconazole technical material, 1-10% of fludioxonil technical material, 2-8% of bactericidal synergist, 1-8% of dispersant, 1-5% of wetting dispersant, 0.2-3% of thickening agent, 0.1-1% of preservative, 3-6% of antifreeze, 0.1-3% of film-forming agent, 1-10% of warning color, 0.1-1% of defoaming agent, and the balance of deionized water to make up to 100%.

[0013] The fungicidal synergist is selected from one or more of zinc pyrithione, Trichoderma-chitosan complex, benzothiadiazole (BTH) derivatives, plant-derived terpene alcohols.

[0014] The dispersing agent is selected from one or more of nonylphenol polyoxyethylene, high molecular comb copolymer, glycerol fatty acid polyoxyethylene, polycarboxylate, cresol-formaldehyde sodium salt concentrate, polyoxyethylene alkyl aryl ether, polyoxyethylene lanolin alcohol, alkyl naphthalene sulfonate, naphthalene sulfonate formaldehyde condensate, sodium lignosulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, lignosulfonate, sodium p-hydroxyphenyl lignosulfonate, fatty alcohol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate, dodecyltrimethylammonium salt, alkyl dimethyl benzyl ammonium salt, p-methoxy fatty amido benzene sulfonic acid, p-methoxy fatty amido benzene sulfonic acid salt, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester.

[0015] The wetting dispersing agent is selected from one or more of Morwet D-360 / D-390, Atlox™ 4913, EO-PO block copolymer.

[0016] The thickening agent is selected from one or more of modified carboxypropyl cellulose, gum arabic, animal glue, pectin, xanthan gum, methyl cellulose, ethyl cellulose, sodium carboxymethyl cellulose, magnesium aluminum silicate, sodium alginate, polyethylene glycol, polyvinyl pyrrolidone, polyacrylamide, sodium polyacrylate.

[0017] The preservative is selected from one or more of 1,2-benzisothiazolin-3-one, formaldehyde, sodium benzoate, isothiazolinones, carboxin, polyformaldehyde, sodium citrate, sodium salicylate, potassium sorbate, sodium sorbate.

[0018] The antifreezing agent is selected from one or more of ethylene glycol, propylene glycol, glycerol, inorganic salt, polyethylene glycol, sorbitol.

[0019] The film forming agent is selected from one or more of modified carboxypropyl cellulose, polyvinyl alcohol, acrylic emulsion, gum arabic, animal glue, pectin, xanthan gum, methyl cellulose, ethyl cellulose, sodium carboxymethyl cellulose, sodium alginate, polyethylene glycol, polyvinyl pyrrolidone, polyacrylamide, sodium polyacrylate.

[0020] The warning color is selected from one or more of acid scarlet, rose essence, red paste, brilliant blue, methyl violet, rose pigment, pearl powder.

[0021] The antifoaming agent is selected from one or more of silicone, butyl phosphate, organosilicon, isobutyl phosphate, n-octanol, polyether antifoaming agent, high carbon alcohol, tributyl phosphate, polysiloxane antifoaming agent.

[0022] Preferably, the fungicidal synergist is zinc pyrithione, the wetting dispersant is an EO-PO block copolymer, and the dispersant is a mixture of polycarboxylate and high molecular comb copolymer. The ratio of polycarboxylate and high molecular comb copolymer is 3:1.

[0023] By adopting the technical scheme, the seed treatment suspension agent of clothianidin, propiconazole and fludioxonil is prepared by using specific raw materials and proportioning, and has good seed treatment effect, good suspension performance, excellent low-temperature stability and thermal storage stability. The seed treatment suspension agent prepared by the application can maintain stability under low-temperature conditions, prevent freezing or precipitation, and ensure effective use in cold environments. The seed treatment suspension agent prepared by the application can also maintain stability under high-temperature conditions, avoid decomposition or failure of active ingredients, and ensure long-term storage and use effect.

[0024] The application combines the three effective active ingredients of clothianidin, propiconazole and fludioxonil to provide more comprehensive seed protection, expand the fungicidal spectrum, improve the protection performance, resist multiple pathogenic fungi, improve the disease resistance of seeds, and protect seeds from insect pests.

[0025] On the other hand, the application provides a preparation method of the seed treatment suspension agent containing clothianidin, propiconazole and fludioxonil, which comprises the following steps: S1, weighing the wetting dispersant, the dispersant and the defoaming agent, and adding them to deionized water under shearing conditions, then adding the clothianidin, propiconazole and fludioxonil technical materials and the fungicidal synergist after shearing for 5 minutes, and continuing to shear for 5 minutes; S2, weighing the antifreeze and the thickening agent, and stirring to obtain a dispersion of the thickening agent in the antifreeze; S3, weighing the preservative and mixing it with the mixture obtained in S1 and S2, and continuing to shear for 15 minutes to obtain a suspension slurry; S4, starting the cooling device, and sanding the suspension slurry obtained in S3, wherein the temperature of the first sanding is not higher than 30℃, and the temperature of the second and third sanding is not higher than 35℃; S5, adding the suspension slurry after sanding in S4, the film-forming agent and the warning color to a mixing kettle, shearing for 20 minutes until the particle fineness reaches D98<5μm, and then detecting each index after obtaining a uniform dispersion, and then packaging after passing the quality inspection to obtain the finished product of the seed treatment suspension agent of clothianidin, propiconazole and fludioxonil; Preferably, the thickening agent is dispersed in the antifreeze in advance.

[0026] On the other hand, the application provides the use of the seed treatment suspension agent containing clothianidin, propiconazole and fludioxonil for preventing and treating wheat diseases and insect pests, wherein the diseases are wheat root rot, and the insect pests are wheat aphids.

[0027] By adopting the technical scheme, the seed treatment suspension agent of the application takes clothianidin, propiconazole and fludioxonil as active ingredients, and exhibits remarkable effects on the prevention and treatment of diseases and pests of crops such as wheat. The preparation kills pests and fungi by the synergistic effect of the three active ingredients, clothianidin efficiently prevents and controls underground pests such as grub and wireworm (the effective period is ≥80 days), propiconazole inhibits soil-borne fungi such as fusarium and smut fungi, and fludioxonil blocks the infection of seed coat and root fungi (such as damping-off and root rot), thereby achieving 360° protection during the seed germination period.

[0028] Preferably, the seed treatment suspension agent is used for coating treatment of wheat seeds.

[0029] By adopting the technical scheme, the seed treatment suspension agent of the application adopts the seed coating treatment method, can protect seeds, promote seed germination and growth, and improve the stress resistance and yield of seeds. Seed coating is beneficial to the effective release and action of active ingredients and other auxiliary ingredients, can also prevent the invasion of pathogenic fungi and pests, and improve the disease resistance and pest resistance of seeds. Moreover, the use method of coating treatment is suitable for various crops.

[0030] In summary, the application has the following at least one beneficial technical effect: 1. The added zinc pyrithione in the application destroys the cell membrane integrity of pathogenic fungi by releasing zinc ions, and inhibits the mitochondrial respiratory chain of fungi, especially for seed-borne / soil-borne diseases (such as damping-off and smut caused by rhizoctonia solani); in combination with the slow-release function of the high molecular comb-shaped copolymer, the effective period of the pesticide is prolonged to the key period of seedling growth.

[0031] 2. The high molecular comb-shaped copolymer inhibits the agglomeration and sedimentation of zinc pyrithione particles (particle size control <2 μm) through steric hindrance effect and electrostatic repulsion, so that the suspension rate is increased to more than 98%, the uniformity of the pesticide solution is ensured, and the pipeline passability is suitable for mechanized coating operation. The coating shedding rate is reduced to <3%.

[0032] 3. The application can promote the synthesis of endogenous auxin in seeds, improve the uniformity of seedling emergence and the development of root system, and reduce the stress of low temperature adversity.

[0033] ‌ DETAILED DESCRIPTION The application will be further described below in conjunction with examples, but the application is not limited thereto.

[0034] The various reagents used in the following examples of the application can be obtained by market purchase or self-preparation. In addition, unless otherwise specified, the percentages in the following examples are mass percentages.

[0035] Example 1: 30% clothianidin + ipconazole + flumetover seed treatment suspension concentrate (25.5% + 3% + 1.5%) formulation

[0036] The 30% clothianidin + ipconazole + flumetover seed treatment suspension concentrate was prepared according to the preparation method described in the summary of the invention.

[0037] Example 2: 35.5% clothianidin + ipconazole + flumetover seed treatment suspension concentrate (28.0% + 4.5% + 3.0%) formulation

[0038] The 35.5% clothianidin + ipconazole + flumetover seed treatment suspension concentrate was prepared according to the preparation method described in the summary of the invention.

[0039] Example 3: 30% clothianidin + ipconazole + flumetover seed treatment suspension concentrate (25.5% + 3% + 1.5%) formulation

[0040] The 30% clothianidin + ipconazole + flumetover seed treatment suspension concentrate was prepared according to the preparation method described in the summary of the invention.

[0041] Comparative Example 1: 8.8% clothianidin + flumetover (8% + 0.8%) seed treatment suspension concentrate formulation

[0042] The 8.8% clothianidin + flumetover (8% + 0.8%) seed treatment suspension concentrate was prepared according to the preparation method described in the summary of the invention.

[0043] Comparative Example 2: 28% ipconazole + clothianidin (3% + 25%) seed treatment suspension concentrate formulation

[0044] The 28% ipconazole + clothianidin (3% + 25%) seed treatment suspension concentrate was prepared according to the preparation method described in the summary of the invention.

[0045] Comparative Example 3: 30% ipconazole + flumetover (20% + 10%) seed treatment suspension concentrate formulation

[0046] The 30% ipconazole + flumetover (20% + 10%) seed treatment suspension concentrate was prepared according to the preparation method described in the summary of the invention.

[0047] Test Example 1 Field trial on control of wheat root rot by 30% clothianidin · ipconazole · flumetover seed treatment suspension Tested wheat variety: Jinghua 11.

[0048] Controlled object: Root rot ( Bipolaris sorokiniana ).

[0049] Test site: Yuzishan Village, Shandongzhuang Town, Pinggu District, Beijing. The soil is yellow soil with pH value of 7. The previous crop is corn. The test site has a history of wheat root rot.

[0050] Plot area: 20 m 2 (5 m x 4 m).

[0051] Number of repetitions: 4 times.

[0052] Start date of the test: October 26, 2023 (sample weighing) Test completion date: June 02, 2024 (last investigation) Technical basis: “NY / T 1464.16-2007 Guidelines for Pesticide Field Trials Part 16: Control of Wheat Root Rot by Fungicides”.

[0053] Quality basis: “Pesticide Registration Test Quality Management Specification” and relevant standard operating procedures of the institution.

[0054] Weather data On the day of sowing (October 27, 2023), it was overcast to sunny, with a temperature of 5-24℃, an average relative humidity of 64%, and a west wind of 1 level.

[0055] Data on other pesticides for controlling pests, weeds and diseases Before the test: No other pesticides were applied.

[0056] During the test: On March 28, 2024, 3% disulfoton · flutriafol dispersible oil suspension 90 mL / acre was applied to control annual weeds in wheat fields; on May 05, 2024, 10% bifenthrin · clothianidin suspension 25 mL / acre was applied to control wheat aphids.

[0057] Application period and method: seed coating. The seeds of each treatment were coated in the order of test pesticides (low concentration to high concentration) followed by control pesticides. The seed coating machine was cleaned when changing pesticides. Add clean water to the prepared pesticide, mix evenly, dilute to 60 mL (about 2 liters of pesticide slurry per 100 kilograms of seeds), and sow about 375 kilograms per hectare. Add seeds and stir evenly to ensure that the pesticide solution is evenly distributed on the seed surface. After drying in a well-ventilated and cool place, divide the seeds of each treatment evenly into 4 parts for sowing. Sow 100 seeds in a fixed area in each plot to evaluate the effect of each treatment on the emergence rate.

[0058] Method and period of use: 30% clothianidin · prothioconazole · flumetover seed treatment suspension agent can be used for seed coating once before wheat sowing to control wheat root rot. The recommended formulation dosage in the field is 300-350 ml / 100 kg of seeds (effective ingredient dosage: 90-105 g / 100 kg of seeds).

[0059] Investigation time and frequency (1) After the blank control emerged (November 8, 2023): Investigate the emergence number, safety; (2) When the blank control is fully diseased (June 2, 2024): Investigate the number of diseased plants, total plant number, disease index, and safety.

[0060] Investigation method (1) Emergence rate investigation: 100 seeds are sown quantitatively in a fixed area per plot, and the emergence rate of the fixed area is investigated after the blank control emerges.

[0061] (2) Effect investigation: When the blank control is fully diseased, five points are randomly sampled per plot, and 1 meter of double rows is investigated per point to investigate the disease at the stem base. The total plant number and the number of plants at each level are recorded.

[0062] The classification standard is as follows: 0 level: No disease spot at the stem base; 1 level: Disease spot around the stem base less than 25%; 3 level: Disease spot around the stem base 26%-50%; 5 level: Disease spot around the stem base 51%-75%; 7 level: Disease spot around the stem base more than 76%, the plant wilts, and is close to death or dead.

[0063] (3) Safety: Observe whether the pesticide has phytotoxicity to crops and affects other non-target organisms by visual observation. The phytotoxicity classification method is: -: No phytotoxicity; +: Mild phytotoxicity, which does not affect the normal growth of crops; ++: Obvious phytotoxicity, which can recover and will not cause crop yield reduction; +++ : High phytotoxicity, which affects the normal growth of crops and causes a certain degree of loss of crop yield and quality; ++++: Severe phytotoxicity, which hinders the growth of crops and causes serious loss of crop yield and quality.

[0064] Method for calculating efficacy According to the investigation results, the control effect is calculated according to the following formula:

[0065]

[0066]

[0067]

[0068] Data statistics method: use excel for data input and statistics, variance analysis uses duncan's new complex range (DMRT) method (the software used is DPS 9.01), for the data containing less than 30% or more than 70% in the control effect, all data are converted by inverse sine square root when variance analysis, but the average number of converted data is restored to the original value when explaining the result of variance analysis.

[0069] Table 1 test agent

[0070] Test results: Control effect: 30% clothianidin. propiconazole. fludioxonil seed treatment suspension agent for controlling wheat root rot in the dosage of 300~350 milliliter / 100 kilograms of seeds (effective ingredient dosage: 90~105 grams / 100 kilograms of seeds), the blank control area fully developed when the control effect of wheat root rot can reach 79.88%~83.18%, better than the control agent 25 grams / liter fludioxonil seed treatment suspension agent and 20% propiconazole suspension seed coating agent, equivalent to 27% phenyl ether. roq. thiamine suspension seed coating agent, which can effectively control wheat root rot.

[0071] The emergence rates of test agent treatments A1, A2 and A3 were 91.25%, 90.50% and 92.75% respectively, and the emergence rates of control agent treatments B, C and D and the blank control treatment were 91.25%, 92.25%, 91.25% and 90.50% respectively.

[0072] Safety: during the test period, the test agent was safe to wheat within the concentration range of this test; had no adverse effects on other non-target organisms.

[0073] Table 2 control effect of different agents on wheat root rot

[0074] Note: the control effect is the average value of each repetition, and different small / large letters represent significant difference at 0.05 / 0.01 level.

[0075] Table 3 effect of different agents on wheat emergence rate

[0076] Test example 2 Field trial on control effect of 30% clothianidin·prothioconazole·flumetover seed treatment suspension concentrate on aphids in wheat Test crop: Wheat (Triticum aestivum) (variety: Jinghua No. 11).

[0077] Control object: Aphids: mainly Rhopalosiphum padi (Linnaeus) Sitobion avenae , with a small amount of Schizaphis graminum (Rondani) Schizaphis graminum . Test site: Yuzishan Village, Shandongzhuang Town, Pinggu District, Beijing, with yellow soil with pH value of 7, the previous crop being corn, and the test plot having a history of aphid occurrence. The investigation was started in the peak period of aphids in the blank control area.

[0078] Plot area: 20 m 2 (5 m x 4 m).

[0079] Number of repetitions: 4 times.

[0080] Method of application: seed coating. The seeds were coated with the test agents (low concentration to high concentration) first, and then the control agents, and the seed coating machine was cleaned when the agents were changed. An appropriate amount of water was added to the prepared agents, mixed evenly to form a slurry (2 liters of slurry per 100 kilograms of seeds), and the seeds were coated with the slurry using the seed coating machine to ensure that the liquid was evenly distributed on the surface of the seeds. After drying in a well-ventilated and cool place, the seeds were evenly divided into 4 parts for sowing. Each plot was sown with 100 seeds in a fixed area to evaluate the effect of each treatment on the emergence rate.

[0081] Start date of the test: October 26, 2023 (sample weighing) Completion date of the test: May 26, 2024 (last investigation) Water and fertilizer management of the test plot Irrigation: full irrigation on October 24, 2023, November 21, 2023, March 20, 2024, and April 28, 2024.

[0082] Fertilization: 40 kg / mu of black gold zinc humic acid mixed fertilizer was applied on October 27, 2023, and 20 kg / mu of urea was applied on March 20, 2024.

[0083] Meteorological data On the day of sowing, it was overcast to sunny (October 27, 2023), with air temperature of 5-24℃, average relative humidity of 64%, and west wind of 1st grade.

[0084] Data of agents for controlling other pests, diseases and weeds Current crop (from planting to the end of the test): 2024-03-28, 3% difenzoquat + flutriafol dispersible oil suspension 90 ml / acre was sprayed to control annual weeds in wheat field; 2024-05-08, 430 g / L tebuconazole suspension concentrate 20 ml / acre was sprayed to control wheat rust and powdery mildew.

[0085] Investigation time and frequency (1) After germination (2023-11-08): investigate the germination rate and safety of fixed planting points; (2) Aphid initial peak period in the blank control area (2024-05-11, wheat heading stage): investigate the number of insect pests and safety; (3) Aphid peak period in the blank control area (2024-05-26, wheat milk stage): investigate the number of insect pests and safety.

[0086] Investigation method (1) Germination rate: 100 seeds were quantitatively sown in a fixed area in each plot, and the number of germinated seeds in the fixed planting points of each plot was investigated after germination.

[0087] (2) Insect pest investigation: in the initial peak period and peak period of aphids in the blank control area, 5-point sampling method was used in each plot, 10 plants were investigated in each point, and a total of 50 plants were investigated to investigate the number of aphids.

[0088] (3) Safety: visual observation of the effects of pesticides on crops and other non-target organisms, and the grading method of pesticide damage is: -: no phytotoxicity; +: mild phytotoxicity, which does not affect the normal growth of crops; ++: obvious phytotoxicity, which can be recovered and will not cause crop reduction; +++ : high phytotoxicity, which affects the normal growth of crops and causes a certain degree of loss of crop yield and quality, and generally requires compensation for part of the economic loss; ++++: severe phytotoxicity, which hinders the growth of crops and causes serious loss of crop yield and quality, and economic loss should be compensated.

[0089] Pesticide efficacy calculation method According to the investigation results, the germination rate and control effect were calculated according to the following formula:

[0090]

[0091] Data statistical method: data input and statistics were performed by excel, and Duncan's multiple range test (DMRT) was used for variance analysis (the software used was DPS 9.01), and for the data less than 30% or more than 70% in the control effect, all data were subjected to inverse sine square root conversion when performing variance analysis, but the average number of converted data was restored to the original value when explaining the results of variance analysis.

[0092] Table 4 Test agent

[0093] Table 5 Dose setting of test agent

[0094] Application method: seed coating (2 liters of pesticide slurry per 100 kilograms of seeds), and the sowing amount was about 375 kilograms per hectare.

[0095] Biostatistical analysis method: Duncan's multiple range test (DMRT) was used to analyze the test data (the software used was DPS 9.01).

[0096] Method of use and period: example 1 controls wheat aphids, and can be coated once on seeds before wheat sowing, and the recommended field preparation amount of the preparation is 300-350 milliliters per 100 kilograms of seeds (the amount of active ingredient is 90-105 grams per 100 kilograms of seeds).

[0097] Test conclusion: (1) In the initial peak period of aphids in the blank control area, the control effects of test agents A1, A2 and A3 were 82.61%, 86.39% and 88.95% respectively, and the control effect of the control agent B was 87.65%. The results of variance analysis showed that the difference in control effect between treatment A1 and treatment B was extremely significant, the difference in control effect between treatment A2, A3 and treatment B was not significant, the difference in control effect between treatment A1 and treatment A2 was significant at the 5% level, and the difference in control effect between treatment A1 and treatment A3 was extremely significant, and the difference in control effect between treatment A2 and treatment A3 was not significant.

[0098] (2) In the peak period of aphids in the blank control area, the control effects of test agents A1, A2 and A3 were 78.88%, 83.49% and 86.65% respectively, and the control effect of the control agent B was 85.01%. The results of variance analysis showed that the difference in control effect between treatment A1 and treatment B was extremely significant, the difference in control effect between treatment A2, A3 and treatment B was not significant, the difference in control effect between treatment A1 and treatment A2 was significant at the 5% level, and the difference in control effect between treatment A1 and treatment A3 was extremely significant, and the difference in control effect between treatment A2 and treatment A3 was not significant.

[0099] Safety: The test agent was safe to wheat at the concentration range of this test during the test period; no adverse effects on other non-target organisms were observed, and it had a synergistic effect on wheat root rot.

[0100] Table 6 Control effects of different agents on wheat aphids

[0101] Note: The control effect is the average value of each repetition, and different small / large letters represent significant differences in control effect at the 0.05 / 0.01 level.

[0102] The emergence rates of test agent treatments A1, A2, and A3 were 90.50%, 92.25%, and 91.00%, respectively, showing excellent control effects. The emergence rates of control agent treatment B and the blank control treatment were 91.25% and 91.50%, respectively.

[0103] Table 7 Effects of different agents on wheat emergence rate

[0104] Test Example 3 The pH value, suspension rate, low-temperature stability, and heat storage stability of the seed treatment suspension concentrate containing clothianidin + prothioconazole + flumetover were detected according to GB / T 17768-1999 "Standard Writing Specification for Suspension Concentrate Products".

[0105] The seed treatment suspension concentrates prepared in Examples 1-3 and Comparative Examples 1-3 were detected for suspension rate, coating shedding rate, low-temperature stability, and heat storage stability, and the detection results are shown in Table 8.

[0106] From the detection results in Table 8, it can be seen that the seed treatment suspension concentrates containing clothianidin + prothioconazole + flumetover prepared in Examples 1-3 have high suspension rate, good suspension performance, good low-temperature stability and heat storage stability, and high uniformity of coating.

[0107] Comparative Example 1 did not add polycarboxylate, EO-PO block copolymer, and high molecular comb copolymer, and the suspension rate of the seed treatment suspension concentrate containing clothianidin + prothioconazole + flumetover prepared thereby was significantly reduced, the suspension performance was poor, and the cold and heat storage stability was poor. The film-forming property was unqualified. The inventors speculate that the possible reason is that the effective ingredients of the seed treatment suspension concentrate are not uniformly mixed, and the stability and uniformity are poor.

[0108] The seed treatment suspension concentrate containing clothianidin + prothioconazole + flumetover prepared in Comparative Example 2 without adding polycarboxylate and high molecular comb copolymer had a significantly reduced suspension rate, poor suspension performance, and poor low-temperature stability.

[0109] The seed treatment suspension of Comparative Example 3 does not add polycarboxylate and EO-PO block copolymer, and the suspension performance, cold and hot storage stability and film forming property are all unqualified.

[0110] Table 8 Quality index detection of seed treatment suspension

[0111] The present application is not limited to the above-mentioned embodiments, on the basis of the technical solutions disclosed in the present application, those skilled in the art can make some substitutions and deformations to some technical features according to the disclosed technical content without creative labor, and these substitutions and deformations are all within the protection scope of the present application.

Claims

1. A seed treatment suspension comprising thiamethoxam, prothioconazole, and fludioxonil, characterized in that, It contains the following components by weight percentage: 10-40% thiamethoxam technical, 1-15% prothioconazole technical, 1-10% fludioxonil technical, 2-8% bactericidal synergist, 1-8% dispersant, 1-5% wetting and dispersing agent, 0.2-3% thickener, 0.1-1% preservative, 3-6% antifreeze, 0.1-3% film-forming agent, 1-10% warning color, 0.1-1% defoamer, and the balance is deionized water to make up to 100%. The bactericidal synergist is zinc pyrithione; The dispersant is a polycarboxylate and a high molecular weight comb copolymer in a ratio of 3:1; The wetting and dispersing agent is an EO-PO block copolymer.

2. The seed treatment suspension according to claim 1, characterized in that, The weight ratio of thiamethoxam, prothioconazole, and fludioxonil is 22-28:1-5:1-3.

3. The seed treatment suspension agent according to claim 1, characterized in that, The thickener is selected from one or more of the following: modified carboxypropyl cellulose, gum arabic, animal glue, pectin, xanthan gum, methylcellulose, ethylcellulose, sodium carboxymethyl cellulose, magnesium aluminum silicate, sodium alginate, polyethylene glycol, polyvinylpyrrolidone, polyacrylamide, and sodium polyacrylate. The preservative is selected from one or more of 1,2-benzisothiazolin-3-one, formaldehyde, sodium benzoate, isothiazolinones, Kathon, polyoxymethylene, sodium citrate, sodium salicylate, potassium sorbate, and sodium sorbate. The antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, inorganic salts, polyethylene glycol, and sorbitol; The film-forming agent is selected from one or more of the following: modified carboxypropyl cellulose, polyvinyl alcohol, acrylic emulsion, gum arabic, animal glue, pectin, xanthan gum, methylcellulose, ethylcellulose, sodium carboxymethyl cellulose, sodium alginate, polyethylene glycol, polyvinylpyrrolidone, polyacrylamide, and sodium polyacrylate. The warning color is selected from one or more of the following: Acid Red, Rose Essence, Red Pigment, Brilliant Blue, Methyl Violet, Rose Red Pigment, and Pearl Powder; The defoamer is selected from one or more of the following: silicone, butyl phosphate, organosilicon, isobutyl phosphate, n-octanol, polyether defoamers, higher alcohols, tributyl phosphate, and polysiloxane defoamers.

4. The seed treatment suspension agent according to claim 1, characterized in that, The suspending agent is composed of the following components: Thiamethoxam technical grade 25.5%, Prothioconazole technical grade 3.0%, Fludioxonil technical grade 1.5%, Zinc pyrithione 5.0%, Polycarboxylate 3.0%, EO-PO block copolymer 2.0%, 1.0% of high molecular weight comb copolymer Magnesium aluminum silicate 0.8%, Xanthan gum 0.1%, 1,2-Benzisothiazolin-3-one 0.1%, Ethylene glycol 4.0%, Modified carboxypropyl cellulose 0.6%, Red pigment 6.0%, Organosilicon 0.2%, Add deionized water to make up the remaining amount.

5. The seed treatment suspension according to claim 1, characterized in that, The suspending agent is composed of the following components: Thiamethoxam technical grade 28.0%, Prothioconazole technical grade 4.5%, Fludioxonil technical grade 3.0%, Zinc pyrithione 5.0%, Polycarboxylate 4.5%, EO-PO block copolymer 2.5%, 1.5% of high molecular weight comb copolymer Polyethylene glycol 0.8%, Xanthan gum 0.1%, Sodium benzoate 0.1%, Ethylene glycol 4.0%, Sodium carboxymethyl cellulose 0.6%, Red pigment 6.0%, Tributyl phosphate 0.2%, Add deionized water to make up the remaining amount.

6. The seed treatment suspension according to claim 1, characterized in that, The suspending agent is composed of the following components: Thiamethoxam technical grade 25.5%, Prothioconazole technical grade 3.0%, Fludioxonil technical grade 1.5%, Zinc pyrithione 5.0%, Polycarboxylate 3.0%, EO-PO block copolymer 2.5%, 1.0% of high molecular weight comb copolymer Sodium alginate 1.0%, Xanthan gum 0.1%, Sodium sorbate 0.5%, Ethylene glycol 4.0%, Ethyl cellulose 1.0%, Red pigment 7.0%, Butyl phosphate 0.2%, Add deionized water to make up the remaining amount.

7. A method for preparing a seed treatment suspension as described in claim 1, characterized in that, The preparation steps include the following: S1. Weigh out the wetting and dispersing agent, dispersant, and defoamer, add them to deionized water under shear conditions, shear for 5 minutes, then add thiamethoxam, prothioconazole, fludioxonil technical and bactericidal synergist, and continue shearing for 5 minutes. S2. Weigh out the antifreeze and thickener, and stir to form a dispersion of thickener in antifreeze; S3. Weigh the preservative and mix it with the mixture obtained from S1 and S2. Continue shearing for 15 minutes to obtain the suspension slurry. S4. After turning on the cooling device, the slurry obtained in S3 is sand-milled. During the sand-milling process, the temperature of the first-stage sand milling does not exceed 30℃, and the temperature of the second and third-stage sand milling does not exceed 35℃. S5. Add the suspended slurry after sand milling in S4, the film-forming agent, and the warning color to the mixing tank. Shear for 20 minutes until the particle fineness reaches D98<5μm and a uniform dispersion is formed. Then test each index. After passing the test, package the product to obtain the thiamethoxam·prothioconazole·fludioxonil seed treatment suspension.

8. The use of the seed treatment suspension according to any one of claims 1-7 for the prevention and control of wheat diseases and pests, characterized in that, The disease mentioned is wheat root rot; the pest mentioned is wheat aphid.

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