Animal oocyte transplantation needle

Abstract

The present invention relates to the field of animal oocyte transplantation, and discloses an animal oocyte transplantation needle, which comprises: a transplantation frame provided with an adjustment auxiliary mechanism; the adjusting auxiliary mechanism comprises a vertical support, a top plate, a sliding plate, a vertical plate, an adjusting plate, a universal ball, a connecting rod, a first arc-shaped clamping sleeve, a second arc-shaped clamping sleeve, an injector and a needle body. The vertical support is fixedly installed at the top of the transplanting frame, the bottom of the top plate is fixedly connected with the vertical support, the sliding plate is installed on the top plate in a sliding mode, the adjusting plate is vertically installed on the vertical plate in a sliding mode, and the universal ball is movably installed at the top of the adjusting plate. The device has the following advantages and effects that puncture bleeding and ovary injury can be relieved through the small-diameter beveled glass needle body, the stable supporting structure is matched with the follicle wall difficult to puncture, handheld operation is replaced with precise adjustment and distance setting, the transplantation precision is improved, the process can be simplified, the technical threshold can be lowered, and the core operation problem in oocyte transplantation is effectively solved.

Description

Technical Field

[0001] This invention relates to the field of animal oocyte transplantation technology, and particularly to an animal oocyte transplantation needle. Background Technology

[0002] In modern livestock breeding systems, especially cattle and sheep, assisted reproductive technologies have effectively shortened generation intervals and enabled the rapid propagation of high-quality genotypes. However, in the practical application of assisted reproductive technologies such as embryo transfer, limitations in equipment and facilities at some livestock farms make it difficult to complete in vitro culture of animal oocytes, and the propagation efficiency of using embryo transfer alone needs improvement. The emerging intrafollicular oocyte transfer (IVT) technology, as a promising assisted reproductive method, involves surgically transferring oocytes collected from live or freshly slaughtered female animals into the follicles of recipient animals in estrus, allowing the donor oocytes to complete subsequent development and eventually ovulate within the recipient follicles. Compared to other assisted reproductive technologies for animals, intrafollicular transfer (IVT) utilizes the recipient's natural follicular environment to support oocyte development, which can, to some extent, replace in vitro oocyte culture technology, thus freeing relevant units from dependence on equipment such as CO2 incubators. Furthermore, IVT allows the transplantation of oocytes obtained from freshly slaughtered female animals and superovulated young animals, helping to broaden the sources of donor oocytes and improve propagation efficiency, while reducing the economic costs of animal propagation. In addition, this technology enables the continuous completion of a series of physiological processes, including ovulation, fertilization, and early embryonic development, within the recipient's body, providing new possibilities for the future development of assisted reproductive technologies for livestock.

[0003] However, during oocyte transfer, puncturing the follicles of recipient animals in estrus often results in severe bleeding, and the follicle walls of certain animal species are sometimes difficult to puncture, posing a significant challenge to the oocyte transfer procedure. Furthermore, current oocyte transfer procedures largely rely on operators holding syringes, which requires improved precision. Therefore, a new animal oocyte transfer needle is needed to address these issues. Summary of the Invention

[0004] The purpose of this invention is to provide an animal oocyte transfer needle to solve the above-mentioned problems.

[0005] The above-mentioned technical objective of the present invention is achieved through the following technical solution: an animal oocyte transfer needle, comprising: A transplantation frame, wherein the transplantation frame is provided with an adjustment auxiliary mechanism; The adjustment auxiliary mechanism includes a vertical support, a top plate, a sliding plate, a vertical plate, an adjustment plate, a universal ball, a connecting rod, an arc-shaped clamping sleeve one, an arc-shaped clamping sleeve two, a syringe, and a needle body; The upright support is fixedly installed on the top of the transplant frame. The bottom of the top plate is fixedly connected to the upright support. The sliding plate is slidably installed on the top plate. The adjusting plate is vertically slidably installed on the vertical plate. The universal ball is movably installed on the top of the adjusting plate. The connecting rod is fixedly installed between the universal ball and the first arc-shaped clamping sleeve. The first arc-shaped clamping sleeve and the second arc-shaped clamping sleeve are fixedly connected by bolts. The first arc-shaped clamping sleeve and the second arc-shaped clamping sleeve are clamped and engaged with the syringe. The needle body is threadedly installed on the syringe.

[0006] A further configuration of the present invention is as follows: the adjustment auxiliary mechanism further includes a base frame, a lead screw, a mounting plate, and a limiting block. The base frame is fixedly installed at the bottom of the vertical plate, the lead screw is rotatably installed on the base frame, the lead screw is threadedly connected to the adjustment plate, the top of the mounting plate is fixedly connected to the top plate, the top of the limiting block is fixedly connected to the adjustment plate, and the limiting block is engaged with the mounting plate.

[0007] A further configuration of the present invention is as follows: a distance setting component is provided on the top plate, the distance setting component includes an extension plate, a guide plate, a sleeve plate and a clamping screw, the bottom of the extension plate is fixedly connected to the slide plate, the guide plate is fixedly installed on the top of the top plate, the sleeve plate and the extension plate are slidably sleeved on the outside of the guide plate, the clamping screw is threaded on the sleeve plate and abuts against the guide plate.

[0008] A further feature of the present invention is that the transplantation frame is provided with an adsorption assembly, the adsorption assembly including an air pump, an air tube and a suction cup, the air pump is fixedly connected to the air tube, the air tube is fixedly installed on the transplantation frame, and the bottom of the air tube is fixedly connected to the suction cup.

[0009] A further feature of the present invention is that a control lever is fixedly mounted on the top of the skateboard.

[0010] A further feature of the present invention is that the needle body is made of high borosilicate glass.

[0011] A further feature of the present invention is that a limiting hole is provided at the top of the mounting plate, and the limiting block is engaged with the limiting hole.

[0012] A further feature of the present invention is that the top of the slide plate has an extension hole, and the top of the extension plate moves out of the extension hole.

[0013] The beneficial effects of this invention are: 1. The needle body of this invention is made of high borosilicate glass with a narrowed tip design. The narrow tip reduces the cutting area on the follicle wall during puncture, and the high smoothness of the glass material reduces frictional damage to the ovarian tissue during puncture, fundamentally reducing bleeding after follicle puncture in recipient animals during estrus and decreasing the risk of postoperative inflammation and infection. The smaller opening in the follicle wall caused by the narrow tip reduces the reflux of follicular fluid after puncture, thereby improving the recovery rate of transplanted oocytes. Furthermore, the transparent glass material of the needle body allows for direct observation of the oocyte transplantation process. 2. The transplantation frame of this invention is fixed by suction cup, avoiding shaking during hand operation; the adjustment auxiliary mechanism can precisely control the position of the needle body through the lead screw and the control lever. With the pointed design of the thin end of the needle body, it can concentrate pressure to accurately break through thick-walled follicles, eliminating the need for repeated puncture attempts, reducing follicle rupture or ovarian damage caused by multiple punctures, and improving the success rate of oocyte transplantation. Attached Figure Description

[0014] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary. The structures, proportions, sizes, etc., drawn in this specification are only used to complement the content disclosed in the specification for those skilled in the art to understand and read, and are not intended to limit the conditions under which the present invention can be implemented. Therefore, they have no substantial technical significance, and any modification of the structure, change of the proportional relationship, or adjustment of the size is not permitted.

[0015] Figure 1 This is a schematic diagram of the structure of an animal oocyte transfer needle proposed in this invention. Figure 1 .

[0016] Figure 2 This is a schematic diagram of the structure of an animal oocyte transfer needle proposed in this invention. Figure 2 .

[0017] Figure 3 This is a schematic diagram of the structure of an animal oocyte transfer needle proposed in this invention. Figure 3 .

[0018] Figure 4 This is a schematic diagram of the structure of an animal oocyte transfer needle proposed in this invention. Figure 4 .

[0019] Figure 5 yes Figure 4 A schematic diagram of part A in the diagram.

[0020] Figure 6 yes Figure 4 A schematic diagram of part B in the diagram.

[0021] In the diagram, 1. Transplant frame; 2. Support; 3. Top plate; 4. Slide plate; 5. Vertical plate; 6. Adjustment plate; 7. Universal ball; 8. Connecting rod; 9. Arc-shaped clamping sleeve one; 10. Arc-shaped clamping sleeve two; 11. Syringe; 12. Needle body; 13. Base frame; 14. Lead screw; 15. Step plate; 16. Limiting block; 17. Extension plate; 18. Guide plate; 19. Sleeve plate; 20. Clamping screw; 21. Extension hole; 22. Air pump; 23. Air tube; 24. Suction cup. Detailed Implementation

[0022] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "circumferential," and "radial," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0024] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more unless otherwise explicitly specified.

[0025] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0026] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0027] See Figures 1-6 The present invention provides an animal oocyte transfer needle, comprising: Transplantation frame 1, which is equipped with an adjustment auxiliary mechanism; The adjustment auxiliary mechanism includes a vertical support 2, a top plate 3, a sliding plate 4, a vertical plate 5, an adjustment plate 6, a universal ball 7, a connecting rod 8, an arc-shaped clamping sleeve 1 9, an arc-shaped clamping sleeve 2 10, a syringe 11, and a needle body 12; The upright support 2 is fixedly installed on the top of the transplant frame 1, the bottom of the top plate 3 is fixedly connected to the upright support 2, the sliding plate 4 is slidably installed on the top plate 3, the adjusting plate 6 is vertically slidably installed on the vertical plate 5, and the universal ball 7 is movably installed on the top of the adjusting plate 6. It is installed by damping to ensure the stability at a certain position. The connecting rod 8 is fixedly installed between the universal ball 7 and the arc-shaped clamping sleeve 1 9. The arc-shaped clamping sleeve 1 9 and the arc-shaped clamping sleeve 2 10 are fixedly connected by bolts. The arc-shaped clamping sleeve 1 9 and the arc-shaped clamping sleeve 2 10 are clamped and engaged with the syringe 11. The needle body 12 is threadedly installed on the syringe 11. It should be noted that the tip of the needle body 12 is a sharp bevel, and the needle body 12 is thinner at the front and thicker at the back.

[0028] With the above structure, by rotating the syringe 11, the angle of the needle body 12 can be adjusted by the setting of the universal ball 7, so that the needle body 12 can be accurately aligned with the ovary. After the position is adjusted, the distance is set by the distance setting component, and then the slide plate 4 is pushed so that the needle body 12 is accurately inserted into the position of the follicle, ensuring the accuracy of the transplantation.

[0029] Specifically, the adjustment auxiliary mechanism also includes a base frame 13, a lead screw 14, a mounting plate 15, and a limiting block 16. The base frame 13 is fixedly installed at the bottom of the vertical plate 5. The lead screw 14 is rotatably installed on the base frame 13 and is threadedly connected to the adjustment plate 6. The top of the mounting plate 15 is fixedly connected to the top plate 3. The top of the limiting block 16 is fixedly connected to the adjustment plate 6 and is engaged with the mounting plate 15.

[0030] With the above structure, when a transplantation operation is required, rotating the screw 14 causes the adjusting plate 6 to move upward. The adjusting plate 6 drives the limiting block 16 to move upward, causing the limiting block 16 to separate from the mounting plate 15. This releases the restriction on the vertical plate 5, allowing the vertical plate 5 to move to the left. Continuing to rotate the screw 14 causes the adjusting plate 6 to continue moving upward, thus adjusting the height of the needle body 12 according to the position of the ovary.

[0031] Specifically, a distance setting component is provided on the top plate 3. The distance setting component includes an extension plate 17, a guide plate 18, a sleeve plate 19, and a clamping screw 20. The bottom of the extension plate 17 is fixedly connected to the slide plate 4. The guide plate 18 is fixedly installed on the top of the top plate 3. The sleeve plate 19 and the extension plate 17 are slidably sleeved on the outside of the guide plate 18. The clamping screw 20 is threaded on the sleeve plate 19 and abuts against the guide plate 18. The top of the slide plate 4 has an extension hole 21. The top of the extension plate 17 moves out of the extension hole 21, which allows the position of the sleeve plate 19 to be set in advance. The position of the sleeve plate 19 is locked by the clamping screw 20. During the subsequent transplantation process, after the extension plate 17 contacts the sleeve plate 19, the needle body 12 moves to the designated position, thus ensuring the accuracy of the transplantation.

[0032] Specifically, the transplant frame 1 is equipped with an adsorption assembly, which includes an air pump 22, an air tube 23, and a suction cup 24. The air pump 22 is fixedly connected to the air tube 23, which is fixedly installed on the transplant frame 1. The bottom of the air tube 23 is fixedly connected to the suction cup 24. When the air pump 22 is started, the air in the suction cup 24 can be extracted, so that the suction cup 24 can be firmly adsorbed on the worktable, ensuring the stability during operation.

[0033] Specifically, a control lever is fixedly installed on the top of the skateboard 4, and the skateboard 4 is controlled by the control lever.

[0034] Specifically, the needle body 12 is made of high borosilicate glass, which allows for direct observation of the oocyte's location, thus greatly reducing the difficulty of oocyte transfer surgery. In addition, the high borosilicate glass, as the main body of the transfer needle, ensures the needle's strength to a certain extent and allows for multiple uses. The needle body 12 has a thinner tip, which can reduce the degree of follicular hemorrhage during estrus during oocyte transfer and reduce damage to the recipient animal's ovary from the puncture operation. The rear end can be directly connected to a 1ml syringe 11 for convenient replacement by the user. The thinner tip of the needle body 12 reduces puncture damage, while the thicker rear end can store more oocytes and absorb more culture medium, reducing the damage of environmental factors to isolated oocytes.

[0035] Specifically, a limiting hole is provided at the top of the plate 15, and the limiting block 16 is engaged with the limiting hole to limit the needle body 12, so that the needle body 12 is in the set position when not in use.

[0036] Working principle: Before the transplantation operation, place the transplantation frame 1 in the designated position on the workbench, start the air pump 22 of the adsorption component, the air pump 22 draws air from the suction cup 24 through the air tube 23, so that the suction cup 24 is tightly adsorbed on the surface of the workbench, to prevent the transplantation frame 1 from shifting during subsequent operations, and to provide a stable foundation for accurate transplantation. At the same time, place the syringe 11 containing oocytes between the arc-shaped clamping sleeve 1 9 and the arc-shaped clamping sleeve 2 10, tighten the bolts to clamp the syringe 11 with the two clamping sleeves, and then thread the needle body 12 onto the front end of the syringe 11 to complete the assembly of the transplantation tool.

[0037] During the needle angle and height adjustment phase, based on the position and angle of the recipient animal's ovary, the initial limit of the needle 12 is first released. The lead screw 14 of the adjustment auxiliary mechanism is rotated. The lead screw 14 is threaded onto the adjustment plate 6, driving the adjustment plate 6 to rise vertically along the vertical plate 5. The adjustment plate 6 then drives the bottom limiting block 16 to rise synchronously until the limiting block 16 disengages from the limiting hole of the mounting plate 15, releasing the lateral lock of the vertical plate 5. At this point, the sliding plate 4 can be pushed, causing the vertical plate 5, adjustment plate 6, and syringe 11 to move laterally as a whole, initially approaching the ovarian region. Continuing to rotate the lead screw 14, the adjustment plate 6 rises or falls further, causing the syringe 11 and needle 12 to adjust their height synchronously until the needle 12 is approximately aligned with the ovarian height. Then, using the movable characteristics of the universal ball 7, the syringe 11 is manually rotated. The universal ball 7 is fixed by damping, allowing flexible adjustment of the angle of the connecting rod 8, thereby changing the puncture angle of the needle 12, ensuring precise alignment of the needle 12 with the follicle position and avoiding ovarian damage caused by angle deviation.

[0038] To avoid the needle body 12 puncturing too deeply or too shallowly during the puncture distance setting stage, the puncture distance is preset by the distance setting component: slide the sleeve 19 along the guide plate 18, move the sleeve 19 to the corresponding position according to the distance measured before the operation, tighten the clamping screw 20 so that the clamping screw 20 presses against the guide plate 18 and fixes the position of the sleeve 19. At this time, the protruding plate 17 fixed with the slide plate 4 moves with the slide plate 4. When the protruding plate 17 contacts the sleeve 19, it indicates that the needle body 12 has reached the preset puncture depth, providing a distance reference for accurate puncture.

[0039] During the oocyte transfer procedure, pushing the control lever at the top of the slide plate 4 causes the slide plate 4 to slide laterally along the top plate 3, moving the syringe 11 and needle 12 towards the ovary. When the extension plate 17 contacts the sleeve plate 19, pushing the slide plate 4 stops. At this point, the needle 12 is precisely inserted into the follicle to the appropriate depth. Pushing the plunger of the syringe 11 slowly injects the oocytes into the follicle, completing the transfer. Because the needle 12 is made of borosilicate glass, the oocyte injection process can be directly observed, reducing blind spots during operation. Simultaneously, the thin tip reduces bleeding and ovarian damage during follicle puncture.

[0040] After the transplantation is completed during the device reset and storage phase, push the slide plate 4 in the reverse direction to remove the needle 12 from the ovarian area; rotate the screw 14 in the reverse direction, the adjusting plate 6 descends, and the limiting block 16 re-engages into the limiting hole of the mounting plate 15, fixing the position of the vertical plate 5 and the needle 12; turn off the air pump 22, the suction cup 24 becomes loose again, and the transplantation frame 1 can be moved to the storage position, or the syringe 11 and needle 12 can be disassembled for cleaning and disinfection in preparation for the next use.

[0041] The present invention has provided a detailed description of an animal oocyte transfer needle. Specific embodiments have been used to illustrate the principles and implementation methods of the invention. These embodiments are merely illustrative and are intended to help understand the method and core concepts of the invention. It should be noted that those skilled in the art can make various improvements and modifications to the invention without departing from its principles, and these improvements and modifications also fall within the scope of protection of the claims.

Claims

1. An animal oocyte transfer needle, characterized by, The utility model provides a kind of transplant frame (1), which is provided with an adjusting auxiliary mechanism. The adjusting auxiliary mechanism includes a stand (2), a top plate (3), a sliding plate (4), a vertical plate (5), an adjusting plate (6), a universal ball (7), a connecting rod (8), an arc-shaped clamping sleeve (9), an arc-shaped clamping sleeve (10), a syringe (11) and a needle body (12). The stand (2) is fixedly installed at the top of the transplant frame (1), the bottom of the top plate (3) is fixedly connected with the stand (2), the sliding plate (4) is slidably installed on the top plate (3), the adjusting plate (6) is vertically slidably installed on the vertical plate (5), the universal ball (7) is movably installed at the top of the adjusting plate (6), the connecting rod (8) is fixedly installed between the universal ball (7) and the arc-shaped clamping sleeve (9), the arc-shaped clamping sleeve (9) and the arc-shaped clamping sleeve (10) are fixedly connected by bolts, the arc-shaped clamping sleeve (9) and the arc-shaped clamping sleeve (10) are clamped with the syringe (11), and the needle body (12) is threadedly installed on the syringe (11). The adjusting auxiliary mechanism further includes a base frame (13), a lead screw (14), a clamping plate (15) and a limiting block (16), the base frame (13) is fixedly installed at the bottom of the vertical plate (5), the lead screw (14) is rotatably installed on the base frame (13), the lead screw (14) is threadedly connected with the adjusting plate (6), the top of the clamping plate (15) is fixedly connected with the top plate (3), the top of the limiting block (16) is fixedly connected with the adjusting plate (6), and the limiting block (16) is clamped with the clamping plate (15).

2. The animal oocyte transfer needle according to claim 1, wherein The top plate (3) is provided with a distance setting assembly, the distance setting assembly includes an extension plate (17), a guide plate (18), a sleeve plate (19) and a pressing screw (20), the bottom of the extension plate (17) is fixedly connected with the sliding plate (4), the guide plate (18) is fixedly installed at the top of the top plate (3), the sleeve plate (19) and the extension plate (17) are slidably sleeved on the outside of the guide plate (18), the pressing screw (20) is threadedly installed on the sleeve plate (19), and the pressing screw (20) abuts against the guide plate (18).

3. The animal oocyte transfer needle of claim 1, wherein The transplant frame (1) is provided with a suction assembly, the suction assembly includes an air pump (22), an air pipe (23) and a suction disc (24), the air pump (22) is fixedly connected with the air pipe (23), the air pipe (23) is fixedly installed on the transplant frame (1), and the bottom of the air pipe (23) is fixedly connected with the suction disc (24).

4. The animal oocyte transfer needle of claim 1, wherein The top of the sliding plate (4) is fixedly provided with a control lever.

5. The animal oocyte transfer needle of claim 1, wherein The needle body (12) is made of high borosilicate glass material.

6. The animal oocyte transfer needle of claim 1, wherein The top of the clamping plate (15) is provided with a limiting hole, and the limiting block (16) is clamped with the limiting hole.

7. The animal oocyte transfer needle of claim 2, wherein The top of the sliding plate (4) is provided with an extension hole (21), and the top of the extension plate (17) moves out of the extension hole (21).

8. The animal oocyte transfer needle of claim 3, wherein ​

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