Foliar fertilizer additive containing sulfur-rich organic matters and used for improving nutritional value of fruits and vegetables

Abstract

The invention relates to the technical field of foliar fertilizers, in particular to a sulfur-rich organic matter-containing foliar fertilizer additive for improving the nutritional value of fruits and vegetables. The foliar fertilizer additive containing the sulfur-rich organic matter and used for improving the nutritional value of the fruits and vegetables comprises the following raw materials in parts by weight: 20-30 parts of the sulfur-rich organic matter, 5-10 parts of tea polyphenol, 4-7 parts of a growth regulator, 10-14 parts of a slow release agent, 15-18 parts of bentonite and 4-8 parts of a seaweed extract. The sulfur-rich organic matter serves as a core component, the additive aims to provide necessary sulfur-containing amino acid for plants, and the amino acid plays an important role in various physiological processes in the plants and participates in protein and vitamin synthesis. By combining the tea polyphenol, the growth regulator, the slow-release agent and other functional components, the health state of plants can be comprehensively optimized, the content of amino acids and vitamins in fruits and vegetables is increased, and finally the purpose of increasing the nutritional value of the fruits and vegetables is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of foliage fertilizers, and particularly relates to a foliage fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables. BACKGROUND

[0002] With the improvement of economic level and the enhancement of health consciousness, consumers' demand for fruit and vegetable products has shifted from simply pursuing yield to paying dual attention to nutritional quality and flavor characteristics. Researchers are constantly committed to developing various foliage fertilizers and additives. However, traditional foliage fertilizers mainly focus on providing basic nutritional elements (such as nitrogen, phosphorus, potassium) and some trace elements required for plant growth. Although these fertilizers are helpful for the basic growth of crops, they have limited effect on improving the nutritional value of crops.

[0003] Traditional foliage fertilizers are difficult to effectively regulate the accumulation of bioactive substances such as amino acids, vitamins, and polyphenols in fruits, ignore the effective regulation of key metabolic pathways in plants, and may cause imbalance of plant leaf microbial community due to excessive application, indirectly affecting the synthesis of secondary metabolites.

[0004] Most of the foliage fertilizers on the market are not specifically designed to enhance the synthesis and accumulation of specific beneficial compounds in fruits and vegetables, such as the improvement of amino acid content and vitamin content, which is a challenge to meet consumers' demand for high-nutritional-value food. SUMMARY

[0005] (I) Technical problems to be solved In view of the above-mentioned shortcomings and deficiencies of the prior art, the present application provides a foliage fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables, which solves the technical problem of being difficult to effectively improve the nutritional value of fruits and vegetables and improve the taste in the prior art.

[0006] (II) Technical solutions In order to achieve the above-mentioned purposes, the main technical solutions adopted by the present application include: a foliage fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables, a preparation method and an application.

[0007] The foliage fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables comprises the following raw materials in parts by weight: sulfur-rich organic matter: 20-30 parts, tea polyphenol: 5-10 parts, growth regulator: 4-7 parts, slow-release agent: 10-14 parts, bentonite: 15-18 parts, and seaweed extract: 4-8 parts.

[0008] Sulfur-rich organic matter is the core component of the additive, and amino acids are the basic units of protein. Sulfur-rich amino acids such as cysteine, methionine, and taurine contain sulfur elements. These amino acids participate in various physiological processes in plants, including protein synthesis, and help promote the accumulation of soluble sugars and organic acids in fruits and vegetables, thereby improving fruit taste, enhancing nutritional value, and overall yield. These key amino acids can help plants more effectively synthesize proteins, thereby increasing protein content and quality in fruits. Providing essential amino acids helps strengthen the structure and texture of fruits and vegetables, making them more firm and elastic. In addition, sulfur-containing compounds can also affect the flavor of fruits and vegetables, adding some unique aroma and taste, and enhancing the overall eating experience.

[0009] Carbohydrates not only provide energy sources for plants, but also improve soil structure, increase soil water retention and air permeability, thereby benefiting root growth and nutrient uptake, and ultimately affecting the quality and nutritional value of fruits and vegetables.

[0010] Lipids are one of the main components of biological membranes and are essential for maintaining the integrity and function of cell membranes. Healthy cell membranes help plants better cope with environmental changes and improve water and nutrient transport efficiency, thereby supporting the overall health status and productivity of plants. Good plant health status usually leads to higher yield and better quality, including increased content of vitamins, minerals, and other beneficial compounds.

[0011] The present application provides a foliar fertilizer additive for improving the nutritional value of fruits and vegetables, which comprises sulfur-rich organic matter, tea polyphenols, growth regulators, slow-release agents, bentonite, and seaweed extract, etc. The additive is compounded by specific proportions of these components. By providing sulfur-rich amino acids, the additive promotes the synthesis of proteins and vitamins in plants, effectively improving the nutritional value and taste quality of fruits and vegetables. At the same time, combined with growth regulators and slow-release technology, it enhances plant stress resistance and nutrient utilization efficiency, prolongs the effect of fertilizer, reduces the frequency of fertilization, and has the comprehensive effect of safety, environmental protection, quality improvement, and yield increase.

[0012] Tea polyphenols have significant antioxidant properties and can scavenge free radicals in plants. This helps protect the active ingredients in sulfur-rich organic matter from oxidative degradation, ensuring that they can better participate in plant metabolic processes, including protein synthesis and antioxidant defense system construction.

[0013] Growth regulators can regulate plant growth and development, including promoting root growth, enhancing photosynthetic efficiency, and improving nutrient uptake capacity. Optimizing the overall growth status of plants allows them to more effectively utilize the amino acids and other nutrients provided by sulfur-rich organic matter, thereby improving nutritional value and yield.

[0014] As a carrier material, bentonite has good adsorption and air permeability, helping to evenly distribute and slowly release the various components in the additive, including sulfur-rich organic matter. Bentonite also improves soil structure, increases soil aeration and water retention, and helps root absorption of water and nutrients, indirectly supporting the effectiveness of sulfur-rich organic matter.

[0015] Seaweed extract is rich in various minerals, vitamins, plant hormones and other bioactive substances, which can stimulate the immune response of plants, enhance the ability to resist diseases and pests, and promote the growth and development of plants. These characteristics help create a healthier growing environment, allowing plants to fully utilize the nutritional ingredients provided by sulfur-rich organic matter, further enhancing the nutritional value and quality of fruits and vegetables.

[0016] In some embodiments, the sulfur-rich organic matter includes sulfur-rich proteins, which include sulfur-rich amino acids and other amino acids, with a weight ratio of 35%-60%:40-65%. The other amino acids include cysteine, methionine and taurine. The other amino acids include histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, valine, alanine, arginine, aspartic acid, glycine, glutamic acid and serine.

[0017] By matching sulfur-rich amino acids with other important amino acids, this additive can significantly improve the nutritional value and taste quality of fruits and vegetables. Sulfur-rich amino acids help promote the synthesis of various bioactive substances such as glutathione and vitamins in plants, while improving their flavor and texture. In addition, the rational mixture of amino acids can effectively promote the absorption and utilization efficiency of nutrients by plants, increasing the content of vitamins, minerals and other health-promoting ingredients in fruits and vegetables, thereby providing more nutritional value. This not only improves the overall quality of fruits and vegetables, but also makes them more delicious and satisfying, meeting the needs of consumers for high-quality agricultural products.

[0018] In some embodiments, the weight percentage of cysteine, methionine and taurine in the sulfur-rich amino acid is: 30%-40%: 40%-45%: 15%-30%.

[0019] By forming sulfur-rich organic matter from cysteine, methionine and taurine in a certain proportion, the synthesis and accumulation of nutrients in plants can be effectively promoted, significantly improving the nutritional value and taste quality of fruits and vegetables. The sulfur-rich amino acid system under this ratio helps optimize the formation of fruit flavor substances, increase sugar content, vitamin content and total amino acid content, making fruits and vegetables more delicious and tasty.

[0020] In some embodiments, the sulfur-rich organic matter further comprises carbohydrates and lipids and other substances, and the weight ratio of the sulfur-rich proteins, carbohydrates, lipids and other substances is 65-80%:5-10%:10-20%:5-10%.

[0021] In some embodiments, the growth regulator comprises choline chloride and brassinolide, wherein the choline chloride is 2-4 parts and the brassinolide is 2-3 parts; the slow-release agent comprises polyvinyl alcohol and polymalic acid, wherein the polyvinyl alcohol is 6-8 parts and the polymalic acid is 4-6 parts.

[0022] Choline chloride 2-4 parts and brassinolide 2-3 parts are compounded in this ratio, which can play a role in promoting plant cell metabolism and enhancing stress resistance, both stimulating the growth potential of crops and not causing physiological disorders due to excessive stimulation; this ratio helps to improve the transmission and absorption efficiency of sulfur-rich amino acids and other nutrients in the body, achieving the organic unity of nutrition and regulation.

[0023] In the slow-release system, polyvinyl alcohol 6-8 parts as a hydrophobic coating material can effectively delay nutrient release, while polymalic acid 4-6 parts further stabilizes the release curve and improves the soil microenvironment through its good water retention and complexing properties. In this ratio, the slow-release performance of the fertilizer is optimized, the stability and utilization rate of the bioactive ingredients are improved, and a high-efficiency, long-lasting, safe comprehensive regulation system is formed with sulfur-rich organic matter and other functional components.

[0024] In some embodiments, tea polyphenols are preferably 6-9 parts, and growth regulators are preferably 5-6 parts. In the present application, the ratio of tea polyphenols 6-9 parts, growth regulators (choline chloride and brassinolide, a total of 5-6 parts) and sulfur-rich organic matter is designed based on their effects on crop nutrition and taste improvement. Tea polyphenols in the ratio of 6-9 parts can fully exert their antioxidant properties, protect sulfur-containing amino acids in sulfur-rich organic matter from oxidative degradation, and enhance the activity of antioxidant enzyme systems in plants. This ratio ensures sufficient antioxidant capacity to support the overall health of plants. When the growth regulator is added in a ratio of 5-6 parts, it can optimally regulate plant metabolism, promote root development, improve nutrient absorption efficiency, and activate the synthesis and transport of sulfur-containing amino acids and other key substances, thereby further enhancing the utilization efficiency of nutrients by plants. Sulfur-rich organic matter as the core functional component provides abundant sulfur-based amino acids, which are essential for protein synthesis and plant growth. The combination of the three in the above ratio enhances the antioxidant defense system of plants, optimizes nutrient absorption and utilization efficiency, promotes the accumulation of sugar content, vitamins and amino acids, and ultimately significantly improves the nutritional value and taste quality of fruits and vegetables. This precise ratio ensures functional complementarity and effect superposition among the components.

[0025] In some embodiments, slow-release agent: 11-13 parts, bentonite: 16-17 parts.

[0026] The three of sulfur-rich organic matter, slow-release agent 11-13 parts and bentonite 16-17 parts form a stable, controllable, long-acting functional foliar fertilizer system. Sulfur-rich organic matter as the core nutrient source, rich in cysteine, methionine, taurine and other sulfur-containing amino acids and various essential amino acids, can significantly improve the protein synthesis capacity and antioxidant level in plants, and enhance the nutritional value of fruits and vegetables; the slow-release agent is composed of polyvinyl alcohol and polymalic acid, which effectively regulates the release rate of active ingredients at a ratio of 11-13 parts, avoids rapid nutrient loss, prolongs the fertilizer effect period, and improves the utilization rate; bentonite plays a good adsorption and structural support role at an addition amount of 16-17 parts, not only enhances the physical stability and dispersibility of the product, but also promotes the uniform combination and adhesion performance of the slow-release agent and sulfur-rich organic matter. Under the action of the three, the stable slow-release of nutrient substances is realized, and the accumulation of key nutrient components such as amino acids, vitamins and soluble sugars in fruits and vegetables is significantly improved, and the flavor and taste of fruits are improved In some embodiments, seaweed extract: 5-7 parts, tea polyphenol: 6-9 parts.

[0027] Under this ratio, seaweed extract and tea polyphenol act to activate the enzyme system in the plant body, promote the absorption and transformation of nutrients such as nitrogen and sulfur, and improve the synthesis efficiency of sulfur-rich amino acids, thereby enhancing protein content and amino acid balance and vitamin synthesis; tea polyphenol effectively protects the active substances in seaweed extract from oxidative degradation, prolongs its action time in the plant body, and improves bioavailability.

[0028] This combination also complements the controlled-release system formed by the slow-release agent and bentonite: the slow-release agent regulates the release rhythm of nutrients, and bentonite enhances adsorption and dispersibility, so that the functional components of seaweed extract and tea polyphenol more uniformly and durably act on the surface of crops, thereby realizing the optimization of the whole process from nutrient supply to metabolic regulation.

[0029] The synergistic cooperation of seaweed extract and tea polyphenol within the range of 5-7 parts and 6-9 parts not only enhances the stress resistance of plants, but also promotes the accumulation of key nutrient components such as soluble sugar, vitamins, flavor amino acids, etc. in fruits and vegetables, significantly improving the color, sweetness and taste of fruits.

[0030] In some embodiments, the preparation process of sulfur-rich organic matter is as follows: Step 1, use 10 m 3 microbial protein reactor, keep the temperature at 30 ℃; continuously supply mineral salt medium through peristaltic pump, and set the hydraulic retention time to 2.5 days; The mixed gas with volume percentage of 15% CO2, 25% O2, 59% H2 and 1% H2S is introduced into the reactor, and the total gas flow is set to 8000 L / d; the gas is fully mixed with the culture medium by a gas pump and then injected into the microbial protein reactor through a nozzle, and the microbial protein reactor is a spraying type; Step 2, adding HOB-SOB mixed bacterial community and other auxiliary bacterial species to the microbial protein reactor to form a complex microbial system, and the addition amount of the complex microbial system is 10% of the volume of the microbial protein reactor; Step 3, collecting the bacterial cells every 14 days during the culture process, a total of 7 times, and the collected bacterial cells are centrifuged and then freeze-dried to obtain a microbial biomass product rich in protein.

[0031] In some embodiments, the pH of the culture medium is maintained at 7.0 using a 0.2 M phosphate buffer solution; the culture medium formula includes the following components: 0.7-1.0 parts of MgCl2·6H2O, 1.0-1.3 parts of NH4Cl, 1.1-1.5 parts of KH2PO4, 1.6-1.8 parts of Na2HPO4, 0.1-0.5 parts of CaCl2·2H2O.

[0032] The culture medium provides a suitable nutrient environment and physiological conditions for the efficient growth and metabolism of the HOB-SOB mixed bacterial community by scientifically proportioning various mineral elements and stabilizing the pH at 7.0 by combining a 0.2 M phosphate buffer system. The reasonable collocation of key elements such as magnesium, phosphorus, nitrogen, and calcium not only meets the basic needs of microorganisms for mineral nutrients, but also promotes the effective utilization of H2S and improves the yield and quality of microbial single-cell protein. At the same time, the culture medium has good adaptability and stability, can match different nitrogen and sulfur supply modes in different operation stages, plays a key supporting role in realizing the simultaneous recovery and conversion of carbon, nitrogen, and sulfur resources, and has good engineering application prospects and industrialization promotion value.

[0033] In some embodiments, the complex microbial system includes HOB, SOB, and other auxiliary bacterial species, and 10% by volume of the HOB-SOB mixed bacterial community is added to the microbial protein reactor, wherein the HOB is selected from one or more of the following: Pseudmonas, Hydrogenophaga, Xanthobacter, Plesiomonas, Prothomonas, Leminobacter, Nocardia, Corynebacter, Rarobacter, Spirilla, Terrabacte, Geobacillus, Flavobacterium, Fattybacilli, Paracoccus, Bacillus, Aeromonas, Micrococcus, Variovorax, Mycobacterium and Alcaligenes genus. The SOB is selected from one or more of the following: Thiobacillus, Acidithiobacillus, Sulfurovum, Sulfurimonas, Halothiobacillus, Thiomonas and Thiovirga genus. The other auxiliary bacterial species is selected from one or more of the following: Nocardia, Burkholdefia, Sulfuriferula and Thioalkalivibrio genus. The relative abundance of HOB is 30%-40%, the relative abundance of SOB is 40%-50%, and the relative abundance of the other auxiliary bacterial species is 10%-15%.

[0034] The present application is constructed to Hydrogenophaga and Thiobacillus The main function of the complex microbial system supplemented by a variety of functional complementary strains (Xanthobacter, Plesiomonas, Acidithiobacillus, Sulfurovum, Halothiobacillus) Efficient conversion of CO2 and H2S is achieved. HOB, as an autotrophic microorganism, can synthesize microbial protein using H2, CO2 and O2, and SOB uses H2S as an energy source, not only reducing the toxicity of sulfides, but also providing essential sulfur elements for microbial protein synthesis. Therefore, the complex microbial system composed of HOB and SOB is more stable, which is conducive to the stable production of microbial protein. Other auxiliary strains show good ecological adaptability and potential metabolic interaction ability, which helps to enhance system stability and improve nutrient utilization. The complex microbial structure takes into account functionality and ecological diversity, can maintain a stable metabolic state under complex operating conditions, significantly improve the yield and nutritional value of microbial single-cell protein, and has good engineering application potential and industrialization prospects. In the preparation process of sulfur-rich organic matter, HOB and SOB exhibit significant synergistic effect through metabolic functional complementation and ecological interaction. HOB mainly uses H2 and CO2 for autotrophic growth, fixing carbon source to synthesize microbial protein; SOB uses H2S as energy for oxidative metabolism, converting it into sulfate medium components. This division not only effectively reduces the toxicity of H2S in the system, ensuring the stability of the microbial community, but also realizes the simultaneous recovery and conversion of carbon and sulfur resources. Hydrogenophaga and Thiobacillus HOB and SOB represented by

[0035] In the second aspect, the present application provides a preparation method of a foliar fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables, comprising the following steps: Step S1, 2-4 parts of choline chloride are prepared into a 10% aqueous solution, 2-3 parts of brassinolide are prepared into a 5% ethanol solution, and the choline chloride solution and the brassinolide solution are mixed uniformly; Step S2, Mix 20-30 parts of sulfur-rich organic matter, 5-10 parts of tea polyphenol, 15-18 parts of bentonite and 4-8 parts of seaweed extract, and grind with an airflow grinder for 15-20 min to make the particle size of the mixed powder less than 200 mesh; Step S3, Add 30-50 parts of deionized water and 5-10 parts of ethanol to the above crushed mixture, then transfer it to a high-speed homogenizer, and shear stir at 5000-7000 rpm for 10-15 minutes to fully wet and preliminarily disperse the components; Step S4, Slowly add the prepared choline chloride and brassinolide mixed solution in step S1 to the dispersion system in step S3, keep the stirring speed at 300-500 rpm, and continue stirring for 5-10 minutes to make the growth regulator evenly distributed in the system; then let the mixed system stand for 2 hours to make the active ingredients fully combined with the sulfur-rich organic matter carrier; Step S5, Add polyvinyl alcohol 6-8 parts and poly malic acid 4-6 parts to the suspension in step S4 in turn, continue to stir at 300-500 rpm for 5-10 minutes to ensure uniform dispersion of the slow-release agent; then add 0.5-1 part of 10% hydroxypropyl methyl cellulose aqueous solution to prevent particle sedimentation and enhance the stability of the system; Step S6, According to the flowability requirements of the system, supplement deionized water or 10% hydroxypropyl methyl cellulose aqueous solution as needed to adjust the viscosity to 40-80 mPa·s and the pH value to 6-7; filter the final uniform suspension to remove impurities, and then fill it into light-proof containers for sealed storage.

[0036] In step S1, choline chloride is prepared into a 10% aqueous solution, and brassinolide is prepared into a 5% ethanol solution, which are then mixed uniformly and allowed to stand for 30 minutes to activate the plant growth metabolism system. This step not only enhances the absorption and conduction efficiency of the plant to nutrients, but also improves the stability and slow-release performance of the active ingredients, prolonging their action time on the leaf surface.

[0037] In step S2, the uniformity and specific surface area of each component are significantly improved, enhancing their dispersibility and active ingredient release efficiency in the subsequent system. Superfine processing helps to improve the adhesion and penetration ability of additives on the leaf surface, promoting the effective absorption of amino acids and bioactive ingredients by plants.

[0038] In step S3, high shear breaks the agglomerated structure between materials, improving the uniformity and stability of the system, and promoting the release and dissolution of active ingredients, laying a good foundation for the addition of growth regulators and slow-release agents in the subsequent steps. In addition, the addition of appropriate amount of ethanol helps to enhance the solubility of tea polyphenols and other components, and also plays a certain antibacterial role, further ensuring the product quality and storage stability.

[0039] In step S4, the effective integration of the growth regulator and the functional component is ensured, the stability and bioavailability in the system are improved, and good conditions are created for the subsequent introduction of the slow-release agent, thereby enhancing the overall efficacy and durability of the additive.

[0040] In step S5, polyvinyl alcohol, as a hydrophobic coating material, cooperates with polymalic acid with water-retaining and complexing ability to construct a stable slow-release structure, prolong the release time of active ingredients, and improve the fertilizer efficiency. Subsequently, 0.5-1 parts of 10% hydroxypropyl methylcellulose aqueous solution is added to further enhance the rheological properties and physical stability of the system, prevent particle sedimentation, and ensure the uniformity and consistency of the product during storage and use.

[0041] In step S6, the physical stability and rheological properties of the additive are optimized to ensure that it remains uniform and consistent during storage and application; appropriate viscosity helps to improve the adhesion and penetration of the product on the leaf surface, enhancing the absorption efficiency of the plant to active ingredients; and precise pH control is beneficial to maintaining the stability and functionality of bioactive substances.

[0042] The foliar fertilizer additive of the present application finally exists in the form of a suspension. The suspension can effectively accommodate solid components such as sulfur-rich organic matter, bentonite, tea polyphenols, etc. that are difficult to dissolve but have strong functionality, allowing them to be uniformly dispersed in the system and achieve slow-release effect; at the same time, plant growth regulators such as choline chloride and brassinolide can be stably loaded on the surface or inside of the carrier, avoiding premature inactivation and enhancing their bioavailability. The addition of polyvinyl alcohol, polymalic acid, and hydroxypropyl methylcellulose and other aids in the formula not only improves the rheological properties and physical stability of the system, preventing particle sedimentation and caking, but also ensures the uniformity and operational convenience of the product during storage and spraying. In addition, the suspension system is beneficial to the contact and absorption of active ingredients with the leaf surface, improving the photosynthetic efficiency and nutrient accumulation level of fruits and vegetables.

[0043] The preparation method of the foliar fertilizer additive provided by the application significantly improves the stability, biological activity and application effect on fruits and vegetables of the additive by scientifically compounding sulfur-rich organic matter, growth regulators and various functional components, and adopting step-by-step mixing and slow-release forming process. Choline chloride and brassinolide are activated and compounded at a specific concentration, synergistically regulate plant growth and metabolism, enhance photosynthetic efficiency, and promote nutrient accumulation; sulfur-rich organic matter is rich in functional amino acids such as cysteine, methionine and taurine, and can effectively improve the antioxidant capacity and nutritional value of fruits and vegetables in combination with tea polyphenols and seaweed extract, while bentonite as an excellent carrier can enhance the structural stability and dispersibility of the product; the slow-release system composed of polyvinyl alcohol and polymalic acid ensures the persistent release of effective components on the leaf surface, improves the utilization rate and reduces the loss. The whole preparation process is mild and controllable, avoiding the destruction of active ingredients by high temperature, and the granular additive prepared at a temperature not higher than 40℃ has good solubility, adhesion and biocompatibility, facilitating storage and application. The foliar fertilizer additive prepared by the method not only can significantly improve the quality and flavor of fruits and vegetables, but also has the advantages of green safety, environmental friendliness and the like, and is suitable for the green production needs of high-quality agricultural products.

[0044] In a third aspect, the application provides the foliar fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables in the foliar fertilizer.

[0045] (Three) beneficial effects The foliar fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables provided by the application comprises the following raw materials by weight: sulfur-rich organic matter 20-30 parts, tea polyphenols 5-10 parts, growth regulators 4-7 parts, slow-release agent 10-14 parts, bentonite 15-18 parts and seaweed extract 4-8 parts. By introducing sulfur-rich organic matter as the core component, the additive aims to provide plants with necessary sulfur-containing amino acids, which play an important role in various physiological processes in the plant body, such as protein synthesis and vitamin synthesis. In addition, in combination with various functional components such as tea polyphenols, growth regulators, slow-release agents, bentonite and seaweed extract, the health status of plants can be comprehensively optimized, the disease resistance and environmental adaptability of plants can be enhanced, the content of vitamins and amino acids in fruits and vegetables can be increased, and finally the purpose of improving the nutritional value of fruits and vegetables can be achieved. DETAILED DESCRIPTION

[0046] In order to make the content of the application more convenient to understand, the technical solutions of the application will be further described below in combination with specific embodiments, but the application is not limited thereto. Any technology realized based on the above content of the application is included in the scope intended to be protected by the application. Unless otherwise specified, the raw materials, reagents and microbial flora used in the examples are commercially available. The reagents, instruments or operation steps not recorded in this text are routine determinations by those skilled in the art.

[0047] The parts or percentages of the examples are all weight percentages.

[0048] Example 1 The preparation method of the sulfur-rich organic matter comprises the following steps: Step 1, The 10 m 3 The microbial protein reactor is kept at a temperature of 30°C.

[0049] The mineral salt medium is continuously supplied by a peristaltic pump, and the hydraulic retention time is set to 2.5 days. The types and contents of the medium are as follows: The pH of the medium is maintained at 7.0 by using a 0.2M phosphate buffer solution; the AMS medium composition includes the following components: 0.7 parts of MgCl2·6H2O, 1.1 parts of KH2PO4, 1.0 parts of NH4Cl, 1.6 parts of Na2HPO4, and 0.1 parts of CaCl2·2H2O.

[0050] The mixed gas with a volume percentage of 15% CO2, 25% O2, 59% H2, and 1% H2S is introduced into the reactor, and the total gas flow rate is set to 8000 L / d; After the gas is fully mixed with the medium by a gas pump, it is injected into the spray-type microbial protein reactor by a nozzle.

[0051] Step 2, 10% volume fraction of HOB-SOB mixed bacterial community is added to the microbial protein reactor, wherein HOB is selected from Hydrogenophaga genus; SOB is selected from Thiobacillus genus.

[0052] A complex microbial system is constructed with Hydrogenophaga and Thiobacillus as the main components, supplemented by a variety of functional complementary bacterial species, which realizes efficient conversion of H2S.

[0053] The relative abundance of the bacterial community is shown in Table 1.

[0054] Table 1: Distribution of the types and relative abundances of HOB, SOB, and other bacterial species in the microbial protein reactor in Example 1.

[0055]

[0056] Step 3, Centrifugation is performed at a speed of 10000 rpm for 10 minutes, and the cultured bacterial cells are collected every 14 days as a test phase, a total of 10 times, followed by freeze-drying treatment, and finally the sulfur-rich organic matter is obtained.

[0057] The amount of biomass collected is shown in Table 2.

[0058] Table 2: Biomass yield (mg VSS / L) of each test phase in the microbial protein reactor in Example 1.

[0059]

[0060] The trend of biomass yield in the microbial protein reactor shows an initial increase followed by a gradual decline. Specifically, it reaches a peak between the first and third phases, and then shows a general downward trend, with a sharp decline in the eighth phase.

[0061] This is because in the early stage of fermentation, the initial nutrient concentration in the medium is high, providing rich growth conditions, which promotes the rapid proliferation of microorganisms, leading to an increase in biomass yield. As time goes on, even with the continuous replenishment of new nutrients, the competition within the microbial population intensifies, which may lead to some microorganisms being unable to obtain sufficient resources for effective proliferation, thereby affecting the overall biomass yield. Microorganisms produce by-products during metabolism, which inhibit the microorganisms themselves, and metabolic products cannot be removed or converted in time, leading to a sharp decline in the eighth phase, but the first seven phases show a relatively stable and high biomass yield state, therefore, the synthesis time of the subsequent biomass as raw material additive is selected from the first to the seventh phase.

[0062] The content distribution of each component in the biomass of each phase is shown in Table 3.

[0063] Table 3: Content distribution of main components in sulfur-rich organic matter in each test phase in Example 1 (%).

[0064]

[0065] Sulfur-rich protein is the sum of all amino acids.

[0066] In the first to seventh phases, the proportion of sulfur-rich protein gradually decreases from 80% to 65%. As the fermentation process progresses, the accumulation of metabolic products inhibits the microorganisms, affecting their ability to synthesize specific proteins. As the fermentation process develops, the interactions between different strains in the complex microbial system change. The proportion of main functional strains Hydrogenophaga and Thiobacillus and their relationship with other auxiliary strains change, affecting the stability and efficiency of the entire system. Further determination of the abundance changes between each strain is needed, in addition, as the fermentation process progresses, the accumulation of metabolic products may inhibit the microorganisms, affecting their ability to synthesize specific proteins.

[0067] With the decrease of the proportion of sulfur-rich proteins, the proportion of carbohydrates and lipids increases accordingly. This is because when the synthesis of sulfur-rich amino acids is limited, the microorganisms will turn to other metabolic pathways to maintain growth and survival, such as using more carbon sources to generate carbohydrates or storing energy as lipids.

[0068] In the 8th to 10th stages, the proportion of sulfur-rich proteins decreases significantly, while the proportion of carbohydrates and lipids increases significantly. This change indicates the result of system aging or imbalance of microbial community structure. Further, the synthesis time of biomass as a subsequent additive for foliar fertilizer should be selected from the first to seventh stages.

[0069] The amino acid content (mg / g VSS) of each stage is shown in Table 4.

[0070] Table 4: Distribution of sulfur-rich organic matter in each test stage in Example 1 (mg / g VSS).

[0071]

[0072] According to the experimental data analysis, the microbial protein biomass in the first to seventh stages is optimal in composition and functionality, and is suitable as an ideal raw material for foliar fertilizer additives. In this stage, the proportion of microbial proteins is stable, and the proportion of sulfur-rich complex amino acids (cysteine, methionine, taurine) in the total amount of amino acids is also stable, and the proportions in each stage are coordinated. At the same time, the types of amino acids are complete and evenly distributed, which is conducive to the synergistic effect of nutrition. In contrast, the content of microbial proteins and sulfur-rich amino acids in the biomass in the 8th to 10th stages decreases significantly, and the proportion of carbohydrates and lipids increases, and the functionality is significantly weakened. Considering the amino acid composition, functionality and process stability, it is recommended to select the first to seventh stages in the fermentation process as the biomass collection window to ensure the efficiency and consistency of the final product. Again, the synthesis time of biomass as a subsequent foliar fertilizer additive raw material should be selected from the first to seventh stages.

[0073] Example 2 The difference between this example 2 and example 1 is that 10% volume fraction of HOB-SOB mixed microbial community is added to the microbial protein reactor, the microbial community is the same, and the relative abundance is different. The specific relative abundance is shown in Table 5.

[0074] Table 5: Distribution of types and relative abundance of HOB, SOB and other species in the microbial protein reactor in Example 2.

[0075]

[0076] The obtained data are shown in Tables 6, 7 and 8.

[0077] Table 6: Biomass yield (mg VSS / L) of each test stage in the microbial protein reactor in Example 2.

[0078]

[0079] Table 7: Content distribution of main components in sulfur-rich organic matter of each test stage in Example 2 (%).

[0080]

[0081] Table 8: Amino acid content distribution (mg / g VSS) of sulfur-rich organic matter of each test stage in Example 2.

[0082]

[0083] Even if the inter-species abundance in the HOB and SOB is changed, the total abundance of the HOB and SOB meets the requirements, efficient synthesis of microbial protein can be achieved, and the proportion of sulfur-rich complex amino acids therein is stable, meeting the requirements of the present application for functional amino acid composition, and having the characteristics of universality and stability.

[0084] Comparative Example 1 The present comparative example 1 is different from Example 1 in that the same flora is used, the relative abundance of the HOB and SOB is greatly changed, and other bacterial species are within the required relative abundance. The specific relative abundance is shown in Table 9.

[0085] Table 9: Distribution of types and relative abundance of HOB, SOB and other bacterial species in the microbial protein reactor in Comparative Example 1.

[0086]

[0087] The data details are shown in Tables 10, 11 and 12.

[0088] Table 10: Biomass yield (mg VSS / L) of each test stage in the microbial protein reactor in Comparative Example 1.

[0089]

[0090] Table 11: Content distribution of main components in sulfur-rich organic matter of each test stage in Comparative Example 1 (%).

[0091]

[0092] Table 12: Amino acid content distribution (mg / g VSS) of sulfur-rich organic matter of each test stage in Comparative Example 1.

[0093]

[0094] The main difference between Comparative Example 1 and Example 1 is the imbalance of the relative abundance of the microbial community, specifically the over-proportion of HOB (hydrogen-oxidizing bacteria) and the under-proportion of SOB (sulfur-oxidizing bacteria), which breaks the original balance. This change severely inhibits the synthesis ability of sulfur-containing amino acids such as cysteine, methionine and taurine. With the progress of fermentation, the proportion of sulfur-rich proteins continues to decline, and the content of key amino acids also decreases significantly, and the overall nutritional value of the biomass is significantly reduced. The results show that once the proportion of the microbial community deviates from the protection interval, it will directly cause a series of chain reactions such as blocking of functional metabolic pathways, accumulation of by-products, and decrease in system stability, ultimately leading to a decrease in the synthesis efficiency of target products and a decrease in the effect of the foliar fertilizer additive prepared. Therefore, maintaining a reasonable ratio between HOB and SOB is a key prerequisite for efficient synthesis of sulfur-rich organic matter.

[0095] Comparative Example 2 The difference between this comparative example 1 and Example 1 is that the microbial community is the same, and the relative abundance of HOB, SOB and other microbial communities is different. The specific relative abundance is shown in Table 13.

[0096] Table 13: Species and relative abundance distribution of HOB, SOB and other bacteria in the microbial protein reactor in Comparative Example 2.

[0097]

[0098] The data details are shown in Tables 14, 15 and 16.

[0099] Table 14: Biomass yield (mg VSS / L) of each test stage in the microbial protein reactor in Comparative Example 2.

[0100]

[0101] Table 15: Content distribution of main components in sulfur-rich organic matter in each test stage in Comparative Example 2 (%).

[0102]

[0103] Table 16: Amino acid content distribution in sulfur-rich organic matter in each test stage in Comparative Example 2 (mg / g VSS).

[0104]

[0105] In Comparative Example 2, when the abundance of other genera accounts for a large proportion, the complexity of the microbial ecosystem increases, and the competition between different functional bacteria intensifies, resulting in a decrease in system stability, which is manifested as a low overall biomass yield and significant fluctuations. The proportion of sulfur-rich proteins at each stage is unstable, and the proportion of carbohydrate and lipid components increases, reflecting a shift in metabolic pathways. The excessive proportion of the remaining bacterial species disrupts the original ecological balance, which is not conducive to the stable production of microbial biomass with high protein and sulfur amino acid content.

[0106] Example 3 The sulfur-rich organic matter selected in Example 1 is used to prepare a foliar fertilizer. The specific steps include the following: Step S1, 2 parts of choline chloride are prepared into a 10% aqueous solution, and 2 parts of brassinolide are prepared into a 5% ethanol solution. The choline chloride solution and the brassinolide solution are mixed uniformly; Step S2, The sulfur-rich organic matter 20 parts, tea polyphenol 5 parts, bentonite 15 parts, and seaweed extract 4 parts are mixed and pulverized in an air flow pulverizer for 15 minutes to make the particle size of the mixed powder about 180 mesh; Step S3, Add 30 parts of deionized water and 5 parts of ethanol to the above pulverized mixture, and then transfer it to a high-speed homogenizer for shearing stirring at 5000 rpm for 5 minutes to make the components fully wet and initially dispersed; Step S4, Slowly add the mixed solution of choline chloride and brassinolide prepared in Step S1 to the dispersion system of Step S3, keep the stirring speed at 300 rpm, and continue stirring for 5 minutes to make the growth regulator uniformly distributed in the system. Then, let the mixed system stand for 2 hours to make the active ingredients fully combined with the sulfur-rich organic matter carrier; Step S5, Add polyvinyl alcohol 6 parts and poly malic acid 4 parts to the suspension of Step S4 in sequence, continue stirring at 300 rpm for 5 minutes to ensure uniform dispersion of the slow-release agent. Then add 0.5 parts of 10% hydroxypropyl methyl cellulose aqueous solution to prevent particle sedimentation and enhance the stability of the system; Step S6, According to the flowability requirements of the system, supplement deionized water or 10% hydroxypropyl methyl cellulose aqueous solution as needed to adjust the viscosity to between 50 mPa·s, and adjust the pH value of the system to between 6.5. After filtering out impurities through a filter screen, the finally formed uniform suspension is filled into a light-proof container and sealed for storage.

[0107] Example 4 The sulfur-rich organic matter selected in Example 2 is used to prepare a foliar fertilizer.

[0108] Comprising the following steps: Step S1, 4 parts of choline chloride was prepared into a 10% aqueous solution, 3 parts of brassinolide was prepared into a 5% ethanol solution, and the choline chloride solution and the brassinolide solution were mixed uniformly; Step S2, 30 parts of sulfur-rich organic matter, 10 parts of tea polyphenol, 18 parts of bentonite, and 8 parts of seaweed extract were mixed, and a jet mill was used for 20 min to obtain a mixed powder with a particle size of 180 mesh; Step S3, 50 parts of deionized water and 10 parts of ethanol were added to the above-mentioned pulverized mixture, and then transferred to a high-speed homogenizer for shearing stirring at 7000 rpm for 10 minutes to make the components fully wet and preliminarily dispersed; Step S4, The mixed solution of choline chloride and brassinolide prepared in step S1 was slowly added to the dispersion system of step S3, and the stirring speed was kept at 500 rpm for 10 minutes to make the growth regulator uniformly distributed in the system; then the mixed system was left to stand for 2 hours to make the active ingredients fully combined with the sulfur-rich organic matter carrier; Step S5, Polyvinyl alcohol 6-8 parts and poly malic acid 4-6 parts were added to the suspension of step S4 in sequence, and stirring was continued at 500 rpm for 10 minutes to ensure uniform dispersion of the slow-release agent; then 1 part of 10% hydroxypropyl methylcellulose aqueous solution was added to prevent particle sedimentation and enhance the stability of the system; Step S6, According to the flowability requirements of the system, deionized water or 10% hydroxypropyl methylcellulose aqueous solution was added as needed to adjust the viscosity to between 60 mPa·s, and the pH value of the system to between 6.5; the finally formed uniform suspension was filtered through a filter screen to remove impurities, and then filled into light-proof containers for sealed storage.

[0109] Comparative Example 3 This comparative example is the same as example 3, except that the amount of sulfur-rich organic matter added is 35 parts.

[0110] Example 5 The test was carried out in the greenhouse of an organic farm, and the soil was moist soil. The physicochemical properties of the plough layer soil were as follows: organic matter 35.62 g / kg, total nitrogen 0.49 g / kg, available phosphorus 15.6 mg / kg, water content 15.23%, Ec 384.52 μs / cm, and pH 7.51.

[0111] In this embodiment, the control group 1 only applies the conventional foliar fertilizer, while the test group 1 adds the additive prepared in Example 3 according to 10% of the total mass of the diluted foliar fertilizer, and fully mixes it with the conventional foliar fertilizer before application, and the rest of the test conditions are the same. The test group 2 adds the additive prepared in Example 4 according to 10% of the total mass of the diluted foliar fertilizer, and fully mixes it with the conventional foliar fertilizer before application, and the rest of the test conditions are the same. The test group 3 adds the additive prepared in Comparative Example 3 according to 10% of the total mass of the diluted foliar fertilizer, and fully mixes it with the conventional foliar fertilizer before application, and the rest of the test conditions are the same.

[0112] During the fruiting stage of the strawberries, 10 strawberries with consistent maturity were selected from each treatment for fruit quality determination. The determination results of the amino acids are shown in Table 17.

[0113] Table 17: Determination results of the amino acid content in the strawberry fruits of the control group and the test groups (g / 100g).

[0114]

[0115] As can be seen from the above table, after using the foliar fertilizer additive containing specific amino acids, the content of various amino acids in the strawberry fruits is increased. This indicates that the foliar fertilizer additive has a positive effect on increasing the amino acid content in the strawberry fruits. It shows that the plant leaves can absorb the amino acids in the sprayed fertilizer and transport them to other parts, including the fruits. Cysteine and methionine are sulfur-containing amino acids, which are not only the basic units of protein synthesis, but also play an important role in the antioxidant defense system. The sulfur-rich amino acids in the foliar fertilizer can provide more precursor substances for strawberries and promote the synthesis of internal amino acids. The data of the test group 3 is not ideal, which may be due to the excessive sulfur-rich organic matter that breaks the action between the components in the original formula system and interferes with the normal nutrient absorption and synthesis path in the plant body.

[0116] The effects of the soil indicators on the growth of strawberries are shown in Table 18.

[0117] Table 18: Comparison of the soil physical and chemical properties of the control group and the test groups of strawberries.

[0118]

[0119] As can be seen from the above table, a small amount of fertilizer will fall into the soil during spraying, thereby changing the soil properties. The organic matter, available phosphorus and total nitrogen content of the soil are increased, the electrical conductivity is slightly increased, the pH remains stable, and the water content is improved, indicating that the additive improves the soil fertility and water retention capacity to some extent.

[0120] The sulfur-rich organic matter, tea polyphenols, and growth regulators in the additive provide a rich source of nutrients and promote the effective conversion and release of nutrients in the soil. In particular, the presence of sulfur-containing amino acids and microbial active substances enhances the supply of important nutrients such as phosphorus and nitrogen in the soil. The addition of bentonite and seaweed extract improves the physical structure of the soil, increases the soil's air permeability and water retention capacity, and is beneficial to root growth and water management. The slow-release system composed of polyvinyl alcohol and poly malic acid ensures the slow release of nutrients, reduces nutrient loss, and improves fertilizer utilization.

[0121] The types of vitamins in the fruits were determined, and the results are shown in Table 19.

[0122] Table 19: Vitamin content determination results in strawberry fruits of the control and test groups (mg / 100g).

[0123]

[0124] As the core functional component, sulfur-rich organic matter is rich in cysteine, methionine, and taurine, which are key precursors for the synthesis of various antioxidant metabolites in plants, including glutathione and vitamin C precursors. Cysteine is one of the most critical sulfur-containing amino acids in plants, directly involved in the synthesis of glutathione (GSH), which is not only an important component of the plant's endogenous antioxidant system but also regulates the redox state of ascorbic acid (vitamin C) and promotes its accumulation in cells. Chlorocholine and brassinolide in the growth regulator can enhance photosynthetic efficiency and nutrient transport capacity by regulating plant hormone balance, thereby increasing the supply level of carbohydrates and precursor substances, providing sufficient carbon skeleton and energy support for vitamin biosynthesis. Brassinolide is beneficial to the synthesis and accumulation of vitamins and other antioxidant substances. Seaweed extract is rich in natural plant hormones and trace active substances, which can activate the secondary metabolic pathways in plants, especially the synthesis pathway of vitamin E. This additive promotes the accumulation of vitamins in fruits and vegetables from multiple levels by providing key precursor substances for vitamin synthesis, enhancing plant antioxidant defense systems, and optimizing metabolic regulation pathways, significantly improving their nutritional value and quality. Therefore, after applying the additive, the contents of vitamin C and E in strawberry fruits are significantly increased.

[0125] Example 6 The pH value of the test field soil was 6.3, the organic matter content was 20.8 g / kg, the total nitrogen content was 1.2 g / kg, the available phosphorus content was 48.4 mg / kg, and the available potassium content was 360.0 mg / kg.

[0126] In the present example, the control group 2 was only applied with the conventional foliar fertilizer, while the test group 4 was applied with the additive prepared in Example 3 added at 10% of the total mass of the diluted foliar fertilizer, and mixed with the conventional foliar fertilizer, and then applied together, with the same test conditions. The test group 5 was applied with the additive prepared in Example 4 added at 10% of the total mass of the diluted foliar fertilizer, and mixed with the conventional foliar fertilizer, and then applied together, with the same test conditions. The test group 6 was applied with the additive prepared in Comparative Example 3 added at 10% of the total mass of the diluted foliar fertilizer, and mixed with the conventional foliar fertilizer, and then applied together, with the same test conditions.

[0127] At the mature stage of the Chinese cabbage, 10 Chinese cabbages with the same maturity were selected from each treatment for quality determination. The determination results of the amino acids are shown in Table 20.

[0128] Table 20: Determination results of the amino acid contents in the Chinese cabbages of the control group and the test groups (g / kg).

[0129]

[0130] From the above table, it can be seen that after the use of the foliar fertilizer additive containing specific amino acids, the contents of various amino acids in the Chinese cabbage also increased The types of vitamins in the Chinese cabbage were determined, and the results are shown in Table 21.

[0131] Table 21: Determination results of the vitamin contents in the Chinese cabbages of the control group and the test groups.

[0132]

[0133] It can be seen that after using the foliar fertilizer additive containing specific amino acids, the vitamin content in Chinese cabbage also increased. The foliar fertilizer additive contains various functional ingredients such as sulfur-rich proteins and tea polyphenols, which can directly or indirectly provide essential nutrients to plants and promote their healthy growth. Sulfur-rich organic matter, as the core functional component, is rich in cysteine, methionine and taurine, etc. These substances are key precursors for the synthesis of various antioxidant metabolites in plants, including glutathione and vitamin C precursors. For example, cysteine is one of the most critical sulfur-containing amino acids in plants, which is directly involved in the synthesis of glutathione (GSH). GSH is not only an important component of the endogenous antioxidant system in plants, but also regulates the redox state of ascorbic acid (vitamin C) and promotes its accumulation in cells. Tea polyphenols have significant antioxidant properties and can scavenge free radicals in plants, reducing oxidative stress damage to plant cells. This helps to protect the internal environment of plants and is conducive to the synthesis and accumulation of vitamins. Growth regulators such as choline chloride and brassinolide can regulate the growth and development process of plants, including promoting root growth, enhancing photosynthetic efficiency, and improving nutrient absorption capacity. These factors work together to enable plants to more effectively use nutrients for metabolic activities, thereby promoting vitamin biosynthesis. Due to the combined effects of the above factors, Chinese cabbage treated with the specific foliar fertilizer additive showed higher vitamin K and vitamin C content at the mature stage compared to the control group. This indicates that the use of the foliar fertilizer additive indeed helps to improve the nutritional value of crops.

[0134] So far, the embodiments of the present application have been described in detail. In order to avoid obscuring the concept of the present application, some details known in the art are not described. Those skilled in the art can fully understand how to implement the technical solutions disclosed herein according to the above description.

[0135] Although some specific embodiments of the present application have been described in detail through examples, those skilled in the art should understand that the above examples are only for illustration and are not intended to limit the scope of the present application. Those skilled in the art should understand that the above embodiments can be modified or some technical features can be replaced with equivalents without departing from the scope and spirit of the present application. In particular, the technical features mentioned in each embodiment can be combined in any way as long as there is no structural conflict.

Claims

1. A foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables, characterized in that, The raw materials include the following weight parts: sulfur-rich organic matter: 20-30 parts, tea polyphenol: 5-10 parts, growth regulator: 4-7 parts, slow-release agent: 10-14 parts, bentonite: 15-18 parts, and seaweed extract: 4-8 parts.

2. The foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, The sulfur-rich organic matter includes sulfur-rich protein, the sulfur-rich protein includes sulfur-rich amino acid and remaining amino acid, and the weight percentage of the sulfur-rich amino acid and the remaining amino acid is 35%-60%: 40-65%; The sulfur-rich amino acid includes cysteine, methionine and taurine; The remaining amino acid includes histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, valine, alanine, arginine, aspartic acid, glycine, glutamic acid and serine.

3. The foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, In the sulfur-rich amino acid, the weight percentage of cysteine, methionine and taurine is: 30%-40%: 40%-45%: 15%-30%.

4. The foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, The sulfur-rich organic matter also includes carbohydrates, lipids and other substances, and the weight part ratio of the sulfur-rich protein, carbohydrates, lipids and other substances is 65-80%: 5-10%: 10-20%: 5-10%.

5. The foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, The growth regulator includes choline chloride and brassinolide, wherein the choline chloride is 2-4 parts and the brassinolide is 2-3 parts; the slow-release agent includes polyvinyl alcohol and polymalic acid, wherein the polyvinyl alcohol is 6-8 parts and the polymalic acid is 4-6 parts.

6. The method of preparing foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables based on claim 1, characterized by, The preparation process is as follows: Step 1, use 10 m 3 The microbial protein reactor was maintained at a temperature of 30 °C; the mineral salt medium was supplied continuously by means of a peristaltic pump, with a hydraulic retention time set at 2.5 days; A mixed gas with a volume percentage of 15% CO2, 25% O2, 59% H2 and 1% H2S is introduced into the reactor, and the total gas flow is set to 8000 L / d; the gas is fully mixed with the culture medium by a gas pump and then injected into the microbial protein reactor by a nozzle, and the microbial protein reactor is a spraying type; Step 2, the HOB-SOB mixed bacterial community and other auxiliary bacterial species are added to the microbial protein reactor to form a composite microbial system, and the addition amount of the composite microbial system is 10% of the volume of the microbial protein reactor; Step 3, during the cultivation process, the bacterial cells are collected every 14 days, a total of 7 times, and the collected bacterial cells are centrifuged and then freeze-dried to obtain a biomass product rich in protein.

7. The foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 6, characterized in that, The pH of the culture medium is maintained at 7.0 using 0.2M phosphate buffer solution; the mineral salt culture medium formula includes the following components: 0.7-1.0 parts of MgCl2·6H2O, 1.0-1.3 parts of NH4Cl, 1.1-1.5 parts of KH2PO4, 1.6-1.8 parts of Na2HPO4, and 0.1-0.5 parts of CaCl2·2H2O.

8. The foliar fertilizer additive comprising sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 6, characterized in that, The complex microbial system includes HOB, SOB, and other auxiliary bacterial species, wherein HOB is selected from the group consisting of Pseudmonas, Hydrogenophaga, Xanthobacter, Plesiomonas, Prothomonas, Leminobacter, Nocardia, Corynebacter, Rarobacter, Spirilla, Terrabacte, Geobacillus, Flavobacterium, Fattybacilli, Paracoccus, Bacillus, Aeromonas, Micrococcus, Variovorax, Mycobacterium and Alcaligenes one or more of the genus Pseudomonas, SOB is selected from the group consisting of Thiobacillus, Acidithiobacillus, Sulfurovum, Sulfurimonas, Halothiobacillus, Thiomonas and Thiovirga one or more of the genus Bacillus; Other helper bacteria are selected from the group consisting of Nocardia, Burkholdefia, Sulfuriferula and Thioalkalivibrio one or more of the genus Lactobacillus; The relative abundance of HOB is 30-40%, the relative abundance of SOB is 40-50%, and the relative abundance of other auxiliary bacterial species is 10-15%.

9. A method for the preparation of foliar fertilizer additive for the enhancement of nutritional value of fruits and vegetables based on the sulfur-rich organic matter according to claim 1, characterized in that, The method comprises the following steps: Step S1, 2-4 parts of choline chloride are prepared into an aqueous solution with a concentration of 10%, 2-3 parts of brassinolide are prepared into an ethanol solution with a concentration of 5%, and the choline chloride solution and the brassinolide solution are uniformly mixed; Step S2, 20-30 parts of sulfur-rich organic matter, 5-10 parts of tea polyphenol, 15-18 parts of bentonite and 4-8 parts of seaweed extract are mixed, and a jet mill is used for crushing for 15-20 min, so that the particle size of the mixed powder is less than 200 mesh; Step S3, 30-50 parts of deionized water and 5-10 parts of ethanol are added to the above-mentioned crushed mixture, and then transferred to a high-speed homogenizer for shearing stirring at 5000-7000 rpm for 5-10 min, so that each component is fully wetted and preliminarily dispersed; Step S4, The mixed solution of choline chloride and brassinolide prepared in step S1 is slowly added to the dispersion system of step S3, the stirring speed is kept at 300-500 rpm, and the stirring is continued for 5-10 min, so that the growth regulator is uniformly distributed in the system; then the mixed system is left to stand for 2 hours, so that the active ingredients are fully combined with the sulfur-rich organic matter carrier; Step S5, Polyvinyl alcohol 6-8 parts and poly malic acid 4-6 parts are sequentially added to the suspension of step S4, and the stirring is continued at 300-500 rpm for 5-10 min to ensure uniform dispersion of the slow-release agent; then 0.5-1 part of 10% hydroxypropyl methyl cellulose aqueous solution is added to prevent particle sedimentation and enhance the stability of the system; Step S6, According to the flowability requirement of the system, deionized water or 10% hydroxypropyl methyl cellulose aqueous solution is appropriately supplemented to adjust the viscosity to 40-80 mPa·s and the pH value of the system to 6-7; the finally formed uniform suspension is filtered through a filter screen to remove impurities, and then filled into a light-proof container for sealing and storage.

10. Application of the sulfur-rich organic matter-containing foliar fertilizer additive for improving the nutritional value of fruits and vegetables according to any one of claims 1-5 in a foliar fertilizer.

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