Skin care product added with bionic sebum composition and preparation method of skin care product

Abstract

The invention relates to the technical field of skin care preparations, in particular to a skin care product added with a bionic sebum composition and a preparation method of the skin care product, and the skin care product contains simmondsia chinensis seed oil fermented by specific bacillus subtilis, squalane, fermented wheat germ oil and fermented macadamia nut seed oil; the preservation number of the bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) No.31968, and the effect of the raw materials can be improved through fermentation. The skin care product disclosed by the invention can effectively repair a skin barrier and regulate water-oil balance; the fermented vegetable oil contains special components to repair the barrier.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care formulations, in particular to a skin care product added with a biomimetic sebum composition and a preparation method thereof. BACKGROUND

[0002] In the modern skin care field, with the improvement of people's living standards and the continuous upgrading of the pursuit of beauty, the efficacy and quality requirements of skin care products are increasingly stringent. As the first line of defense against the external environment, the skin's barrier function and water-oil balance are crucial. When the skin barrier is damaged or the water-oil balance is unbalanced, a series of problems such as dryness, roughness, sensitivity, and acne may occur, seriously affecting the health and appearance of the skin.

[0003] Traditional skin care products have many limitations in solving these problems. On the one hand, existing moisturizing products often rely on a single moisturizing ingredient such as glycerin, hyaluronic acid, etc. Although they can supplement skin moisture to some extent, they are not effective in deep moisturizing and long-term maintenance of skin hydration. Moreover, these ingredients may not fundamentally repair damaged skin barriers, making the skin prone to moisture loss and sensitivity reactions under external environmental stimuli.

[0004] On the other hand, products on the market that claim to have skin barrier repair functions are mostly based on simple ingredient coverage or physical protection, and fail to effectively intervene in the complex physiological processes of damaged skin barriers. For example, some products add ingredients similar to sebum, but due to the lack of promotion of skin cell metabolism and regeneration, they are difficult to truly repair and strengthen damaged barriers.

[0005] In addition, for the regulation of skin water-oil balance, existing skin care products usually adopt a single strategy of oil control or hydration, and cannot intelligently adjust according to the actual condition of the skin. For example, oil control products may remove too much skin oil, leading to dry skin and stimulating the sebaceous glands to secrete more oil, forming a vicious cycle; while pure hydration products may not be able to regulate the secretion function of the sebaceous glands, and cannot solve the water-oil imbalance problem from the root.

[0006] In terms of raw materials, many skin care products use chemical synthetic ingredients, which have certain efficacy but may irritate the skin and cause allergic reactions and other adverse reactions with long-term use. Moreover, the production process of these chemical synthetic raw materials is often not environmentally friendly, which does not meet the current trend of green and environmentally friendly development.

[0007] In recent years, with the development of biotechnology, the research of biomimetic sebum has gradually become a hot spot. However, the existing biomimetic sebum technology still has some shortcomings. Some biomimetic sebum compositions are not optimized in terms of raw material selection, and the synergistic effect of various components cannot be fully played, resulting in limited comprehensive performance of the product. In terms of preparation process, some technologies are too complex and costly, making it difficult to achieve large-scale industrial production; while others may damage the effective components of the raw materials during fermentation or preparation, affecting the quality and efficacy of the product.

[0008] In summary, the existing skin care products have many problems in skin barrier repair, water and oil balance regulation, and raw materials and process, which cannot meet the needs of consumers for efficient, safe and green skin care products. Therefore, it is of great practical significance to develop a skin care product with innovative biomimetic sebum composition and its preparation method, which not only fills the market gap, but also brings new breakthroughs to the field of skin care. SUMMARY

[0009] (1) Technical problems solved

[0010] In view of the shortcomings of the prior art, the present application provides a skin care product with a biomimetic sebum composition and a preparation method thereof.

[0011] (2) Technical solutions

[0012] A skin care product with a biomimetic sebum composition, comprising the following components by mass percentage: jojoba seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 15-25%; squalane 15-25%; wheat germ oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 15-25%; macadamia nut seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 15-25%;

[0013] The Bacillus subtilis Demeteria Pulchra Ugel-02 was deposited with the China General Microbiological Culture Collection Center on September 13, 2024, and the biological preservation number is CGMCC No. 31968.

[0014] Preferably, the jojoba seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 contains a fatty acid derivative, the mass fraction of which is 0.5-2%, and the fatty acid derivative is γ-hydroxy-hexadecenoic acid ethyl ester, the structural formula of which is:

[0015]

[0016] Preferably, the wheat germ oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 contains antioxidant polypeptides, the mass fraction of which is 1-3%; and the antioxidant polypeptides are composed of glycine, proline and cysteine, and the average molecular weight is 1000-1500 Da.

[0017] Preferably, the macadamia seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 contains flavonoids, the mass fraction of which is 0.8-2.5%, and the flavonoids are 5,7-dihydroxy-3'-methoxy flavone-7-O-glucoside, the structure of which is as follows:

[0018]

[0019] Preferably, the preparation method of the skin care product added with the biomimetic sebum composition comprises the following steps:

[0020] S1: The raw materials are weighed according to the mass percentage, and the raw materials include 15-25% jojoba seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02, 15-25% squalane, 15-25% wheat germ oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02, and 15-25% macadamia seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02;

[0021] S2: The weighed jojoba seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02, wheat germ oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02, and macadamia seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 are added to a stirring container for stirring treatment to obtain a mixed oil phase A;

[0022] S3: The squalane is slowly added to the mixed oil phase A, and the stirring device is started to continue stirring while adding, so that the squalane can be fully integrated into the mixed oil phase A to form a uniform and stable mixed system B;

[0023] S4: The mixed system B is subjected to comprehensive sterilization treatment to remove possible microorganisms and impurities in the system, so as to ensure the safety and stability of the subsequent product;

[0024] S5: The sterilized mixed system B is transferred to a homogenizing device for homogenization treatment, so that the possible small particle aggregation in the system is broken down, and a homogenized system C with fine texture and uniform dispersion is obtained.

[0025] S6: adding preservatives to the homogeneous system C in a certain proportion, starting the stirring device to make the preservatives uniformly distributed in the system, and obtaining semi-finished product D with preliminary preservative effect after stirring is completed;

[0026] S7: subjecting the semi-finished product D to standing and aging treatment to make the components in the system further integrate and stabilize, and filtering the product by using a filtering device to remove a small amount of impurities that may exist, and obtaining the skin care product added with the bionic sebum composition.

[0027] Preferably, the stirring treatment in S2 is carried out at 30-40℃ for 10-20 minutes.

[0028] Preferably, the continuous stirring in S3 is carried out at 45-55℃ for 20-30 minutes.

[0029] Preferably, the sterilization treatment in S4 is ultraviolet sterilization, the sterilization time is 15-25 minutes, and the wavelength of the ultraviolet light is 250-260nm.

[0030] Preferably, the homogenization treatment in S5 is carried out at 25-30℃, the homogenization pressure is 10-15MPa, and the homogenization time is 5-10 minutes.

[0031] Preferably, the preservative in S6 is phenoxyethanol, the addition amount is 0.1-0.3% of the mass of the homogeneous system C, and the stirring condition is stirring at 20-25℃ for 5-10 minutes; the standing and aging condition in step 7 is at 20-25℃ for 24-48 hours.

[0032] (III) Beneficial technical effects

[0033] Compared with the prior art, the beneficial effects of the present application are:

[0034] 1. Natural plant oils fermented by Bacillus subtilis, such as jojoba seed oil, wheat germ oil and macadamia seed oil, produce special fatty acid derivatives, antioxidant polypeptides and new flavonoids during fermentation. These components can penetrate into skin cells, enhance cell-cell connections, repair damaged skin barrier structure, enhance the self-protection ability of the skin, and reduce the damage of external stimuli to the skin.

[0035] 2. The addition of biological enzymes during fermentation promotes the decomposition and fermentation of raw materials, improving fermentation efficiency and product quality. The entire preparation process is mild, maximally preserving the active ingredients in the raw materials, ensuring the safety and effectiveness of the product. Moreover, the preparation method is simple to operate, low in cost, suitable for large-scale industrial production, and can meet the market demand for high-quality skin care products, providing consumers with better and more affordable skin care options. BRIEF DESCRIPTION OF DRAWINGS

[0036] Figure 1 is a flow chart of the preparation method of the skin care product added with the biomimetic sebum composition according to the present application;

[0037] Figure 2 is a columnar comparison chart of the moisturizing performance of the examples and the comparative examples;

[0038] Figure 3 is a line comparison chart of the skin barrier repair performance of the examples and the comparative examples;

[0039] Figure 4 is the nuclear magnetic resonance hydrogen spectrum of the fatty acid derivative ethyl γ-hydroxy-hexadecenoate according to the present application;

[0040] Figure 5 is the nuclear magnetic resonance hydrogen spectrum of the flavonoid compound 5,7-dihydroxy-3'-methoxy flavone-7-O-glucoside according to the present application. DETAILED DESCRIPTION

[0041] According to Figures 1 to 5 , the detailed description of the present application is as follows:

[0042] Example 1

[0043] Preparation of raw materials

[0044] Select granular and non-moldy jojoba seeds, wheat germ, and macadamia nuts, rinse them three times with deionized water, and then mix them in a mass ratio of 1:1.5:1.2. Pour them into a stainless steel container, add deionized water with a total mass of 4 times, soak them at 25℃ for 18 hours, stir them every 6 hours during the soaking process at a stirring speed of 30 revolutions per minute, ensure that the raw materials are fully water-absorbed and swollen, and the water content of the raw materials after soaking should reach more than 200%, and the change in water content is monitored in real time by weighing method.

[0045] Fermentation process

[0046] Transfer the soaked raw materials to a 100L fermentation tank, sterilize them at 121℃ for 25 minutes, cool them to 35℃ by circulating water after sterilization, inoculate them with 1×10 9Bacillus subtilis Demeteria Pulchra Ugel-02 bacterial liquid with CFU / mL, preservation number CGMCC No.31968, inoculation amount 3% of total mass of raw materials. Fermentation for 5 days under the conditions of 180 rpm, 35℃, pH 7.0, and sterile air is introduced into the fermenter every 18 hours during the fermentation process, the aeration amount is 1 times / minute of the volume of the fermenter, and a compound enzyme with a mass fraction of 0.2% of cellulase and protease is added at the same time, and the mass ratio of the compound enzyme is 1:1. The dissolved oxygen content is monitored every 12 hours during the fermentation process, and is maintained above 30%, and the content of ethyl γ-hydroxy-hexadecenoate in the fermented jojoba seed oil is 1.2%, which meets the range of 0.5-2%, and the content of antioxidant polypeptide in the fermented wheat germ oil is 2.1%, which meets the range of 1-3%.

[0047] Oil extraction

[0048] After the fermentation is completed, the product is transferred to a belt dryer, and is air-dried at 40℃ until the water content is less than 8%, and a horizontal spiral cold press is used for cold pressing for 3 hours under a pressure of 25 MPa, and the temperature in the press chamber is controlled below 28℃ by circulating cooling water during the cold pressing process. The extracted oil is filtered through 200 mesh nylon filter cloth and 0.45 μm microporous filter membrane in turn, and fermented jojoba seed oil, wheat germ oil and macadamia seed oil are obtained. The acid value of the filtered oil is ≤1.5 mg KOH / g, and the peroxide value is ≤3.0 mmol / kg, and the content of 5,7-dihydroxy-3'-methoxy flavone-7-O-glucoside in the fermented macadamia seed oil is 1.8%, which is within the range of 0.8-2.5%.

[0049] Mixing of raw materials and treatment

[0050] Preparation of mixed oil phase: 20% fermented jojoba seed oil, 20% squalane, 20% fermented wheat germ oil and 20% fermented macadamia seed oil are added in a 50 L vacuum emulsification kettle according to the mass percentage, the stirring system is started, the stirring speed is 80 rpm, the jacket temperature is controlled at 50℃, and the mixed system B is formed after mixing for 45 minutes. The particle size of the dispersed phase is monitored by a laser particle size analyzer during the period to ensure that 90% of the particle size is ≤5 μm, and the vacuum degassing device is started after the mixed system is uniformly mixed, the vacuum degree is-0.08 MPa, and the degassing is performed for 10 minutes.

[0051] Sterilization treatment: the mixed system B is transferred to an ultraviolet sterilization equipment, and is irradiated by ultraviolet with a wavelength of 254 nm for 20 minutes to remove microorganisms and impurities.

[0052] Homogenization treatment: the sterilized B is cooled to 28℃, and is transferred to a homogenization equipment, and is homogenized at a pressure of 12 MPa for 8 minutes to break the particle aggregation, and the homogenized system C is obtained.

[0053] Antiseptic treatment: 0.2% phenoxyethanol was added to the homogeneous system C, and stirred at 22°C for 8 minutes to make the antiseptic evenly distributed, obtaining semi-finished product D.

[0054] Post-treatment

[0055] The nano pearl powder with a particle size of 80 nm was mixed with silane coupling agent KH-570 at a mass ratio of 100:2 for modification. The infrared spectrum detected a characteristic peak of 1710 cm -1 -1, 1.5% ginseng stem extract, and 0.2% vitamin C palmitate were added to the semi-finished product D, and stirred at 80 rpm for 15 minutes. The mixed system was left to stand at 22°C for 36 hours for aging, and then filtered through a 0.22 μm filter membrane to remove impurities. The filtrate was aseptically filled in an ISO 7 clean room using a piston filler with a filling head temperature of 45°C. After filling, the product was subjected to online weighing with a weight deviation of ≤±1% and microbial detection with a total bacterial count <100 CFU / g and a mold and yeast count <10 CFU / g.

[0056] Example 2

[0057] Raw material preparation

[0058] High-quality jojoba seeds, wheat germ, and macadamia nuts were selected, washed with deionized water, and then mixed at a mass ratio of 1:1:1.5. Deionized water was added at a total mass of 3 times the mass of the raw materials, and the mixture was soaked at 30°C for 12 hours. Sodium pyrophosphate was added as a chelating agent at a concentration of 0.03%, and the soaking solution was replaced every 4 hours. The final moisture content of the raw materials was 180%, and the impurities were removed by filtering through a vibrating screen with a mesh size of 2 mm.

[0059] Fermentation process

[0060] The raw materials were transferred to a 50 L fermenter, sterilized at 121°C for 20 minutes, and then cooled to 30°C. Bacillus subtilis Demeteria Pulchra Ugel-02 bacterial solution was inoculated at a concentration of 1 x 10 9 CFU / mL, and the fermentation was carried out at a shaking speed of 150 rpm, 30°C, and pH 6.5 for 3 days. Sterile air was supplied at a rate of 0.5 times the tank volume per minute every 12 hours, and 0.1% cellulase and protease complex enzymes were added every 8 hours to maintain pH stability. The content of ethyl γ-hydroxy-hexadecenoate in the fermentation product was 0.8%, and the content of antioxidant polypeptide was 1.2%, both of which were within the standard range.

[0061] Oil extraction and mixing treatment

[0062] The fermentation product is air-dried at 35°C to moisture <10%, cold-pressed at 20 MPa for 2 hours, and the oil is filtered through a plate and frame filter with 1 μm filter cloth and 0.22 μm membrane, with peroxide value ≤2.5 mmol / kg and acid value ≤1.0 mg KOH / g. 15% fermented jojoba seed oil, 15% squalane, 15% fermented wheat germ oil, and 15% fermented macadamia seed oil are added in mass percentage, mixed at 40°C and 50 rpm for 30 minutes to form system B, sterilized by ultraviolet light at 250 nm for 15 minutes, homogenized at 25°C and 10 MPa for 5 minutes, 0.1% phenoxyethanol is added and stirred for 5 minutes to obtain semi-finished product D.

[0063] Post-processing

[0064] Modified nano pearl powder with particle size of 50 nm, 0.5% natural plant spice, and 1% ginseng stem extract are added, stirred at 50 rpm for 20 minutes, filtered after standing and aging at 20°C for 24 hours, and filled in a ten-thousand-level clean area. The product is detected to have total bacterial count <100 CFU / g and mold and yeast <10 CFU / g.

[0065] Example 3

[0066] Raw material preparation and fermentation

[0067] Full raw materials are selected and mixed in a mass ratio of 1:2:1, soaked in 5 times of deionized water at 20°C for 24 hours, stirred at 20 rpm, and centrifugally dewatered at 3000 rpm for 10 minutes to moisture <60%. It is moved into a 200 L fermentation tank, sterilized at 121°C for 30 minutes, cooled to 37°C, inoculated with 5% Bacillus subtilis Demeteria Pulchra Ugel-02 bacterial liquid CGMCC No.31968, and fermented at 200 rpm, 37°C, and pH 7.5 for 7 days, with air supply at 1.5 times the tank volume per minute every 24 hours, addition of 0.3% compound enzyme, and 1% glucose supplement on the third day. The fermented oil is detected to have ethyl γ-hydroxy-hexadecenoate 1.8% and flavonoid compounds 2.0%, meeting the standard.

[0068] Oil extraction and processing

[0069] Air-dried at 45°C to moisture <8%, cold-pressed at 30 MPa for 4 hours with press chamber temperature ≤25°C, and filtered through a three-stage filter with 200 mesh filter screen, 0.45 μm, and 0.1 μm membrane. Mixed in a ratio of 25% fermented jojoba seed oil, 25% squalane, 25% fermented wheat germ oil, and 25% fermented macadamia seed oil to form system B, sterilized by ultraviolet light at 260 nm for 25 minutes, homogenized at 30°C and 15 MPa for 10 minutes, and 0.3% phenoxyethanol is added and stirred at 25°C for 10 minutes to obtain semi-finished product D.

[0070] Post-processing

[0071] The modified nano pearl powder with a particle size of 100 nm, 2% natural plant fragrance, and 2% ginseng stem extract were added, stirred at 100 rpm for 30 minutes, and then filtered after standing and aging at 25°C for 48 hours. The product was filled in an ISO 8 clean area. The product passed the high-temperature stability test at 45°C for 4 weeks and the low-temperature test at -10°C for 2 weeks.

[0072] Comparative Example

[0073] The raw materials were not fermented, and 20% jojoba seed oil, 20% squalane, 20% wheat germ oil, and 20% macadamia seed oil were mixed at 50°C and 80 rpm for 45 minutes. Then, 1.5% untreated pearl powder with a particle size of >100 nm, 1% natural fragrance, and 1.5% ordinary plant extract were added, stirred for 15 minutes, and then filled in an ordinary workshop without sterilization and homogenization treatment. After being stored for 1 week, oil and water were separated.

[0074] The moisturizing properties of the examples and the comparative example were compared, as shown in the following table:

[0075] Table 1

[0076]

[0077]

[0078] The table shows the changes in skin moisture content of the examples and the comparative example at different time points. It can be clearly seen that the skin moisture content of Examples 1-3 at each time point after application is higher than that of the comparative example, indicating that the skin care product with the added biomimetic sebum composition has better moisturizing properties and can lock in skin moisture for a longer period of time.

[0079] The skin barrier repair properties of the examples and the comparative example were compared, as shown in the following table:

[0080] Table 2

[0081] Group Example 1 Example 2 Example 3 Comparative Example Skin transepidermal water loss (TEWL) initial value (g / m 2 / h) 20 21 20 20 TEWL value (g / m 2 / h) after 1 week of application 15 16 14 18 TEWL values (g / m 2 2 weeks after application 12 13 11 16 TEWL values (g / m 2 / h) after 3 weeks of application 10 11 9 14

[0082] This table presents the changes in trans-epidermal water loss (TEWL) of the examples and the comparative example. The lower the TEWL value, the better the skin barrier function. From the data, it can be seen that the TEWL values of Examples 1-3 at each week after application are lower than those of the comparative example, indicating that the skin care product of the present application can more effectively repair the skin barrier and reduce the loss of skin moisture.

[0083] Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A skincare product containing a biomimetic sebum composition, characterized in that, By weight percentage, it contains the following components: 15-25% jojoba seed oil fermented with Bacillus subtilis Demeteria Pulchra Ugel-02; 15-25% squalane; 15-25% wheat germ oil fermented with Bacillus subtilis Demeteria Pulchra Ugel-02; and 15-25% macadamia seed oil fermented with Bacillus subtilis Demeteria Pulchra Ugel-02. Among them, Bacillus subtilis Demeteria Pulchra Ugel-02 was deposited at the China General Microbiological Culture Collection Center on September 13, 2024, with the biological accession number CGMCC No. 31968.

2. The skincare product with added biomimetic sebum composition according to claim 1, characterized in that, The jojoba seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 contains a fatty acid derivative with a mass fraction of 0.5-2%, wherein the fatty acid derivative is γ-hydroxy-hexadecenoic acid ethyl ester, and its structural formula is:

3. The skincare product with added biomimetic sebum composition according to claim 1, characterized in that, The wheat germ oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02 includes antioxidant peptides with a mass fraction of 1-3%; and the antioxidant peptides are composed of glycine, proline, and cysteine, with an average molecular weight of 1000-1500 Da.

4. The skincare product with added biomimetic sebum composition according to claim 1, characterized in that, The macadamia seed oil fermented with Bacillus subtilis (Demeteria Pulchra Ugel-02) contains flavonoids at a mass fraction of 0.8-2.5%, wherein the flavonoid is 5,7-dihydroxy-3'-methoxyflavonol-7-O-glucoside, and its structure is as follows:

5. A method for preparing a skincare product containing a biomimetic sebum composition as described in claim 1, characterized in that, Includes the following complete steps: S1: Weigh the raw materials according to the mass percentage, the raw materials including 15-25% jojoba seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02, 15-25% squalane, 15-25% wheat germ oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02, and 15-25% macadamia seed oil fermented by Bacillus subtilis Demeteria Pulchra Ugel-02; S2: The weighed jojoba seed oil fermented with Bacillus subtilis Demeteria Pulchra Ugel-02, wheat germ oil fermented with Bacillus subtilis Demeteria Pulchra Ugel-02, and macadamia seed oil fermented with Bacillus subtilis Demeteria Pulchra Ugel-02 are added to a mixing container and stirred to obtain mixed oil phase A; S3: Slowly add squalane to the mixed oil phase A while turning on the stirrer to continue stirring, so that the squalane can be fully incorporated into the mixed oil phase A to form a homogeneous and stable mixed system B; S4: Perform comprehensive sterilization on the mixed system B to remove any microorganisms and impurities that may be present in the system, ensuring the safety and stability of subsequent products; S5: Transfer the sterilized mixture B to a homogenizer for homogenization. The homogenization process breaks up any small particle aggregation that may exist in the system, resulting in a homogenized system C with a fine texture and uniform dispersion. S6: Add preservative to homogeneous system C in a certain proportion, turn on the stirring device to make the preservative evenly distributed in the system, and after stirring, obtain semi-finished product D with preliminary anti-corrosion effect. S7: The semi-finished product D is subjected to static aging treatment to allow the components in the system to further integrate and stabilize. After aging, a filtration device is used to filter it to remove any possible small amount of impurities. The filtrate is then used to obtain the skin care product containing the added biomimetic sebum composition.

6. The method for preparing a skincare product with an added biomimetic sebum composition according to claim 5, characterized in that, The stirring conditions in S2 are stirring at 30-40℃ for 10-20 minutes.

7. The method for preparing a skin care product with an added biomimetic sebum composition according to claim 5, characterized in that, The conditions for continued stirring in S3 are to raise the temperature to 45-55℃ and stir for 20-30 minutes.

8. The method for preparing a skin care product with an added biomimetic sebum composition according to claim 5, characterized in that, The sterilization process in S4 is performed using ultraviolet (UV) sterilization for 15-25 minutes at a wavelength of 250-260 nm.

9. The method for preparing a skin care product with an added biomimetic sebum composition according to claim 5, characterized in that, The homogenization conditions in S5 are as follows: cool the sterilized mixture B to 25-30℃, homogenize at a pressure of 10-15MPa, and homogenize for 5-10 minutes.

10. The method for preparing a skin care product with an added biomimetic sebum composition according to claim 5, characterized in that, In step S6, the preservative is phenoxyethanol, and the amount added is 0.1-0.3% of the mass of the homogenized system C. The stirring conditions are stirring at 20-25℃ for 5-10 minutes. In step 7, the conditions for standing aging are a temperature of 20-25℃ and a time of 24-48 hours.

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