Private care antibacterial soothing wet tissue containing Ectoin

Abstract

The invention discloses a private care antibacterial soothing wet tissue containing Ectoin, and relates to the technical field of wet tissues. The private care antibacterial soothing wet tissue comprises a base material and a liquid composition, wherein the liquid composition comprises Ectoine or Ectoine-loaded composite hydrogel, lactic acid bacteria fermentation filtrate, tea tree essential oil, decyl glucoside, a purslane extract, asiaticoside and the balance of water; the pH value of the liquid composition is 3.8 to 4.5; and the base material is spunlace non-woven fabric. The invention also provides a preparation method of the Ectoine-containing private care antibacterial soothing wet tissue, and the preparation method comprises the following steps: firstly preparing the liquid composition, then soaking the spunlace non-woven fabric in the liquid composition, and then folding, sealing and packaging to obtain the Ectoine-containing private care antibacterial soothing wet tissue. The wet tissue provided by the invention not only has good bacteriostasis and antibacterial properties, but also can provide a good private mucous membrane repairing effect and reduce the red and swollen rate of private parts.

Description

Technical Field

[0001] This invention relates to the field of wet wipes technology, and more particularly to a feminine care antibacterial and soothing wet wipe containing ectoine. Background Technology

[0002] The female genital area is an important organ for metabolism and secretion. Its environment is complex and susceptible to various pathogens due to internal and external factors, making it highly vulnerable to infections and diseases. This not only affects women's physical and mental health and quality of life but can even lead to infertility. Furthermore, the skin in the genital area is sensitive and prone to various inflammations or simple itching, requiring special care and nourishment. Most existing wet wipes only provide basic cleaning, with limited antibacterial properties and may even irritate the skin in the genital area, failing to adequately meet the needs of female consumers. Therefore, developing a dedicated wet wipe for the care of the female genital area is a pressing issue that needs to be addressed by those skilled in the art.

[0003] CN103340816A discloses a feminine hygiene wipe specifically designed for women. The wipe's formula comprises a nano-silver solution, water-soluble vitamin E, hyaluronic acid, lauric acid-modified superoxide dismutase, chamomile hydrosol, pearl hydrolysate, soapberry extract, 1,2-propanediol, isothiazolinone, and deionized water, prepared in a specific ratio to form a wipe stock solution. This stock solution is then evenly applied to a spunlace nonwoven fabric membrane and sealed in packaging. This invention not only sterilizes and disinfects the female intimate area but also moisturizes and cleanses the skin, with no side effects or irritation. However, this feminine hygiene wipe does not provide a good repair effect on the intimate mucosa and is less effective in reducing redness and swelling.

[0004] CN104042558A discloses a functional feminine hygiene wipe and its preparation method. It is made by injecting the following raw materials into a spunlace nonwoven fabric: functional probiotics, propylene glycol, polyhexamethylene biguanide, moisturizing agents, essential oils, and purified water. The feminine hygiene wipe provided by this invention can effectively inhibit the growth of harmful bacteria, reduce itching and inflammation in the female private parts caused by harmful bacterial infections, and effectively protect the health of the female vulva and reproductive tract. It is non-irritating and non-allergenic, safe and effective. However, it does not maintain the acid-base balance of the female private parts; prolonged use may disrupt the acid-base balance, leading to a reduction in beneficial bacteria and increasing the risk of disease in women. Summary of the Invention

[0005] In view of the above-mentioned deficiencies of the prior art, the present invention provides a feminine care antibacterial and soothing wipe containing ectoine and its preparation method. The wipe not only has good antibacterial properties, but also provides a good effect on repairing the feminine mucosa, reducing the rate of feminine redness and swelling, and avoiding the problem of disrupting the acid-base balance of the female feminine, with low irritation to the female feminine, thus well meeting the needs of female consumers.

[0006] To achieve the above objectives, the present invention provides a feminine hygiene antibacterial and soothing wipe containing ectoine, comprising a substrate and a liquid composition, wherein the liquid composition comprises, by weight percentage: 0.1%-1% ectoine or ectoine-loaded composite hydrogel, 1%-3% lactic acid bacteria fermentation filtrate, 0.05%-0.1% tea tree oil, 0.06-0.12% decyl glucoside, 0.03-0.08% purslane extract, 0.02-0.05% asiaticoside, with the remainder being water.

[0007] Preferably, the substrate is a spunlace nonwoven fabric.

[0008] Preferably, the pH value of the liquid composition is 3.8-4.5.

[0009] Preferably, the mass ratio of the substrate to the liquid composition is 1:2-4.

[0010] Preferably, the preparation method of the ectoin-loaded composite hydrogel includes the following steps: S1. Add 9.5-10.5g of lysozyme to 85-95mL of water and mix. Adjust the pH to 2-2.2 with 3-6mol / L HCl aqueous solution and bring to a final volume to obtain a lysozyme solution. Then heat the lysozyme solution at 75-78℃ for 8-12h, followed by centrifugation at 10000-13000 rpm for 15-25 min at 1-5℃. Collect the precipitate, mix the precipitate with 8-12mmol / L Bis-Tris buffer, and homogenize at 7000-10000 rpm for 3-6 min to obtain a solution with a precipitate content of 4-6wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 8-12mmol / L... Prepare a 0.5-2 wt% epigallocatechin gallate solution in Bis-Tris buffer; then mix the epigallocatechin gallate solution with an equal volume of lysozyme amyloid cellulose solution and stir for 20-30 min to form a solution containing epigallocatechin gallate complex. S2. Add ectoine powder to water to prepare an ectoine solution with a concentration of 8-12% w / v; weigh 1.8-2.2g of dried porcine type I collagen and add it to 85-95mL of pre-cooled 0.1-0.3mol / L acetic acid at 1-5℃, stir magnetically at 50-200rpm for 1-2h, and bring to a final volume to obtain a collagen solution; then mix the ectoine solution and collagen solution prepared above at a volume ratio of 1:8-10, and stir magnetically at 23-27℃ and 100-200rpm for 20-30min to obtain an ectoine-loaded collagen solution; mix the ectoine-loaded collagen solution with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 3-5:1, then add 8-9mL of 9-10mmol / L genipin ethanol aqueous solution, and stir magnetically at 23-27℃ and 100-200rpm for 20-30min to obtain an ectoine-loaded collagen solution; mix the ectoine-loaded collagen solution with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 3-5:1, then add 8-9mL of 9-10mmol / L genipin ethanol aqueous solution, and stir magnetically at 23-27℃ and 100-200rpm for 20-30min. Stir magnetically at rpm for 20-30 minutes; then let stand at 35-38℃ to form a hydrogel, incubate overnight at 3-6℃, then rinse, freeze dry, pulverize and sieve for later use.

[0011] Preferably, step S2 may also be: Ectoin powder was added to water to prepare an ectoin solution with a concentration of 8-12% w / v. 1.8-2.2 g of dextran amino acid was weighed and added to 95-105 mL of water, and the mixture was magnetically stirred at 200-300 rpm for 0.5-1.5 h to obtain a dextran amino acid solution. The ectoin solution and the dextran amino acid solution were then mixed at a volume ratio of 1:8-10, and magnetically stirred at 23-27℃ and 100-200 rpm for 20-30 min to obtain an ectoin-loaded dextran amino solution. The ectoin-loaded dextran amino solution was mixed with the epigallocatechin gallate complex solution from step S1 at a volume ratio of 3-5:1, and then 8-9 mL of a 9-10 mmol / L genipin ethanol aqueous solution was added. The mixture was stirred at 23-27℃ and 100-200 rpm for 20-30 min to obtain an ectoin-loaded dextran amino solution. Stir magnetically at rpm for 20-30 minutes; then let stand at 35-38℃ to form a hydrogel, incubate overnight at 3-6℃, then rinse, freeze dry, pulverize and sieve for later use.

[0012] Preferably, the lactic acid bacteria fermentation filtrate is obtained by fermenting *Lactobacillus plantarum* N13 (accession number CGMCC No. 20496) to obtain the fermentation broth, followed by filtration. *Lactobacillus plantarum* N13 is a publicly disclosed strain, as disclosed in CN114869917A.

[0013] This invention also provides a method for preparing the above-mentioned feminine hygiene antibacterial and soothing wipes containing ectoine, comprising the following steps: Step 1: Weigh out ectoine, lactic acid bacteria fermentation filtrate, tea tree oil, decyl glucoside, purslane extract, asiaticoside, and water, then mix the substances to prepare a liquid composition. Alternatively, weigh out the following: ectoin-loaded composite hydrogel, lactic acid bacteria fermentation filtrate, tea tree oil, decyl glucoside, purslane extract, asiaticoside, and water, then mix the substances to prepare a liquid composition. Step 2: Immerse the sterilized and dried substrate in the above liquid composition, then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

[0014] The beneficial effects of this invention are: 1. Compared with existing technologies, this invention adjusts and optimizes the formula of the liquid composition for feminine hygiene antibacterial and soothing wipes, rationally proportioning each raw material. The resulting wipes effectively moisturize and clean the female private parts, are safe and non-irritating, and will not damage the vaginal mucosa. While each component of the wipe compound liquid exerts its own effect, they synergistically promote each other, exhibiting excellent antibacterial effects. This reduces odor, itching, and inflammation caused by harmful bacterial infections in the female private parts, regulates and maintains the ecological balance of the female private part's flora, and effectively protects the health of the female vulva and reproductive tract.

[0015] 2. Compared with the prior art, the present invention uses ectoine in combination with lactic acid bacteria fermentation filtrate to enhance the repair of the private mucosa through the synergistic effect of the two. On this basis, it is further improved by introducing a solution containing epigallocatechin gallate complex and collagen or dextran amino acid mixture to load ectoine to prepare ectoine-loaded composite hydrogel. This not only further enhances the antibacterial effect, but also significantly improves the retention rate of ectoine, thereby significantly improving the repair efficacy of the private mucosa and reducing the rate of redness and swelling in the private area. Detailed Implementation

[0016] The parameters and sources of some substances in the examples are as follows: Lactic acid bacteria fermentation filtrate was obtained by fermenting *Lactobacillus plantarum* N13 (CGMCC No. 20496) and then filtering it. The specific method for preparing the lactic acid bacteria fermentation filtrate is as follows: The preserved *Lactobacillus plantarum* N13 strain (CGMCC No. 20496) was inoculated onto MRS solid medium (10 g / L peptone, 10 g / L beef extract, 5 g / L yeast extract, 20 g / L glucose, 5 g / L sodium acetate, 2 g / L diammonium citrate, 2 g / L K₂HPO₄, 0.2 g / L MgSO₄·7H₂O, 0.05 g / L MnSO₄·H₂O, 15 g / L agar, pH 6.5±0.2) and incubated at 37℃ for 48 h for activation. Single colonies were picked and inoculated into MRS liquid medium and cultured at 37℃ and 200 rpm with shaking for 24 h until the OD₆₀ value reached 1.0 and the viable count in the seed culture was ≥1×10⁻⁶. 8 CFU / mL; the seed culture was inoculated at a rate of 5% (v / v) into sterilized fermentation medium (20 g / L glucose, 10 g / L peptone, 5 g / L yeast extract, 5 g / L sodium acetate, 2 g / L diammonium citrate, 2 g / L K₂HPO₄, 0.2 g / L MgSO₄·7H₂O, 0.05 g / L MnSO₄·H₂O, 1 mL / L Tween 80), and fermented at 37℃±1℃ and 100 rpm for 48 h until the OD600 value reached 2.0 and the viable cell count in the fermentation broth was ≥1×10⁻⁶. 9 CFU / mL; After fermentation, the fermentation broth was centrifuged at 10,000 rpm for 15 min at 4℃, and the supernatant was collected; The supernatant was filtered using an ultrafiltration membrane with a molecular weight cutoff of 20 kDa to obtain a clear filtrate; The clear filtrate was sterilized, and then 0.6 wt% butanediol, 0.3 wt% 1,2-hexanediol and 0.1 wt% capryloyl hydroxamic acid were added and mixed, and then filled and sealed to obtain the finished lactic acid bacteria fermentation filtrate; The finished lactic acid bacteria fermentation filtrate is a pale yellow liquid with a fermentation odor, no off-odor and no visible foreign matter, pH 5.0, soluble solids 1.0%, total bacterial count ≤1000 CFU / g, mold ≤20 CFU / g, yeast ≤20 CFU / g, and pathogenic bacteria were not detected.

[0017] Tea tree essential oil, product number: MF-013253, sourced from Xinyang Mufan Biotechnology Co., Ltd. Purslane extract, product specification: 10:1, product number: MF-000563, sourced from Xinyang Mufan Biotechnology Co., Ltd.; Lysozyme, product number: 10837059001, brand: Merck; Porcine type I collagen (dried), purity ≥96%, brand: Chuang'er Biotechnology; Dextran amino, molecular weight: 40000, WH007N031, brand: Weihua Bio; Chitosan, pharmaceutical grade, molecular weight: 300 kDa, commercially available.

[0018] Example 1 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine includes the following steps: Step 1: Weigh out the following ingredients by weight percentage: 1% ectoine, 2.5% lactic acid bacteria fermentation filtrate, 0.08% tea tree oil, 0.1% decyl glucoside, 0.05% purslane extract, 0.04% asiaticoside, and 96.23% water. Mix the ingredients to prepare a liquid composition. The pH value of the liquid composition is 4.2. Step 2: Soak the sterilized and dried spunlace nonwoven fabric in the above liquid composition, wherein the mass ratio of spunlace nonwoven fabric to liquid composition is 1:3. Then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

[0019] Example 2 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine includes the following steps: Step 1: Weigh out the following by weight percentages: 1% loaded ectoine composite hydrogel, 2.5% lactic acid bacteria fermentation filtrate, 0.08% tea tree oil, 0.1% decyl glucoside, 0.05% purslane extract, 0.04% asiaticoside, and 96.23% water. Mix the substances to prepare a liquid composition. The pH value of the liquid composition is 4.2. Step 2: Soak the sterilized and dried spunlace nonwoven fabric in the above liquid composition, wherein the mass ratio of spunlace nonwoven fabric to liquid composition is 1:3. Then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

[0020] The preparation method of the ectoin-loaded composite hydrogel includes the following steps: S1. Add 10g of lysozyme to 90mL of water and mix. Adjust the pH to 2.0 with 6mol / L HCl aqueous solution, and then make up to 100mL with water to obtain a lysozyme solution. Heat the lysozyme solution at 77℃ for 10h, then centrifuge at 12000rpm for 20min at 4℃, collect the precipitate, and mix the precipitate with 10mmol / L Bis-Tris buffer (pH=5.8) and homogenize at 8000rpm for 5min to obtain a solution with a precipitate content of 5wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 10mmol / L Bis-Tris buffer (pH=5.8) to prepare a 1.0wt% epigallocatechin gallate solution. Mix the epigallocatechin gallate solution and the lysozyme amyloid cellulose solution in equal volumes and stir for 30min to form a solution containing epigallocatechin gallate complex. S2. Add ectoine powder to water to prepare an ectoine solution with a concentration of 10% w / v; weigh 2.0 g of dried porcine type I collagen and add it to 90 mL of pre-cooled 0.1 mol / L acetic acid at 4°C, stir magnetically at 100 rpm for 2 h, and then bring the volume to 100 mL with 0.1 mol / L acetic acid to obtain a collagen solution; then mix the ectoine solution and collagen solution prepared above at a volume ratio of 1:9, and stir magnetically at 25°C and 200 rpm for 30 min to obtain an ectoine-loaded collagen solution; mix the ectoine-loaded collagen solution with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 4:1, and then add 8 mL of 10 mmol / L genipin ethanol aqueous solution (ethanol 10% v / v) was magnetically stirred at 25°C and 200 rpm for 30 min; then it was allowed to stand at 37°C to form a hydrogel, and incubated overnight at 4°C. Then it was washed three times with PBS buffer (pH=7.4), freeze-dried, pulverized and passed through a 100-mesh sieve for later use.

[0021] Example 3 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine differs from Example 2 only in that the method for preparing the ectoine-loaded composite hydrogel includes the following steps: S1. Add 10g of lysozyme to 90mL of water and mix. Adjust the pH to 2.0 with 6mol / L HCl aqueous solution, and then make up to 100mL with water to obtain a lysozyme solution. Heat the lysozyme solution at 77℃ for 10h, then centrifuge at 12000 rpm at 4℃ for 20min, collect the precipitate, and mix the precipitate with 10mmol / L Bis-Tris buffer (pH=5.8) and homogenize at 8000rpm for 5min to obtain a solution with a precipitate content of 5wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 10mmol / L Bis-Tris buffer (pH=5.8) to prepare a 1.0wt% epigallocatechin gallate solution. Then mix the epigallocatechin gallate solution and the lysozyme amyloid cellulose solution in equal volumes and stir for 30min to form a solution containing epigallocatechin gallate complex. S2. Add ectoine powder to water to prepare an ectoine solution with a concentration of 10% w / v; weigh 2.0 g of dextran amino acid and add it to 100 mL of water, and stir magnetically at 300 rpm for 1 h to obtain a dextran amino acid solution; then mix the ectoine solution and the dextran amino acid solution prepared above at a volume ratio of 1:9, and stir magnetically at 25℃ and 200 rpm for 30 min to obtain an ectoine-loaded dextran amino acid solution; mix the ectoine-loaded dextran amino acid solution with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 4:1, then add 8 mL of 10 mmol / L genipin ethanol aqueous solution (ethanol 10% v / v), and stir magnetically at 25℃ and 200 rpm for 30 min; then let it stand at 37℃ to form a hydrogel, incubate overnight at 4℃, then wash 3 times with PBS buffer (pH=7.4), freeze-dry, pulverize and pass through a 100-mesh sieve for later use.

[0022] Comparative Example 1 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine differs from Example 2 only in that the method for preparing the ectoine-loaded composite hydrogel includes the following steps: S1. Add 10g of lysozyme to 90mL of water and mix. Adjust the pH to 2.0 with 6mol / L HCl aqueous solution, and then make up to 100mL with water to obtain a lysozyme solution. Heat the lysozyme solution at 77℃ for 10h, then centrifuge at 12000rpm at 4℃ for 20min, collect the precipitate, and mix the precipitate with 10mmol / L Bis-Tris buffer (pH=5.8) and homogenize at 8000rpm for 5min to obtain a solution with a precipitate content of 5wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 10mmol / L Bis-Tris buffer (pH=5.8) to prepare a 1.0wt% epigallocatechin gallate solution. Then mix the epigallocatechin gallate solution and the lysozyme amyloid cellulose solution in equal volumes and stir for 30min to form a solution containing epigallocatechin gallate complex. S2. Ectoin powder was added to water to prepare a 10% w / v ectoin solution; 2.0 g of chitosan was weighed and added to 90 mL of pre-cooled 0.1 mol / L acetic acid at 4°C, and magnetically stirred at 300 rpm for 12 h, then diluted to 100 mL with water to obtain a chitosan solution; the ectoin solution and chitosan solution prepared above were then mixed at a volume ratio of 1:9, and magnetically stirred at 25°C and 200 rpm for 30 min to obtain an ectoin-loaded chitosan solution; the ectoin-loaded chitosan solution was mixed with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 4:1, and then 8 mL of 10 mmol / L genipin ethanol aqueous solution (10% v / v ethanol) was added, and stirred at 25°C and 200 rpm for 30 min to obtain an ectoin-loaded chitosan solution; the ectoin-loaded chitosan solution was mixed with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 4:1, and then 8 mL of 10 mmol / L genipin ethanol aqueous solution (10% v / v ethanol) was added, and stirred at 25°C and 200 rpm for 30 min. Stir magnetically at rpm for 30 min; then let stand at 37°C to form a hydrogel, incubate overnight at 4°C, then wash 3 times with PBS buffer (pH=7.4), freeze dry, pulverize and pass through a 100-mesh sieve for later use.

[0023] Comparative Example 2 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine includes the following steps: Step 1: Weigh out the following components by weight percentage: 0.5% ectoine, 0.5% epigallocatechin gallate complex hydrogel, 2.5% lactic acid bacteria fermentation filtrate, 0.08% tea tree oil, 0.1% decyl glucoside, 0.05% purslane extract, 0.04% asiaticoside, and 96.23% water. Mix these components to prepare a liquid composition. The pH value of the liquid composition is 4.2. Step 2: Soak the sterilized and dried spunlace nonwoven fabric in the above liquid composition, wherein the mass ratio of spunlace nonwoven fabric to liquid composition is 1:3. Then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

[0024] The preparation method of the epigallocatechin gallate composite hydrogel includes the following steps: S1. Add 10g of lysozyme to 90mL of water and mix. Adjust the pH to 2.0 with 6mol / L HCl aqueous solution, and then make up to 100mL with water to obtain a lysozyme solution. Heat the lysozyme solution at 77°C for 10h, then centrifuge at 12000rpm at 4°C for 20min, collect the precipitate, and mix the precipitate with 10mmol / L Bis-Tris buffer (pH=5.8) and homogenize at 8000rpm for 5min to obtain a solution with a precipitate content of 5wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 10mmol / L Bis-Tris buffer (pH=5.8) to prepare a 1.0wt% epigallocatechin gallate solution. Then mix the epigallocatechin gallate solution and the lysozyme amyloid cellulose solution in equal volumes and stir for 30min to form a solution containing epigallocatechin gallate complex. S2. Weigh 2.0 g of dextran amino and add it to 100 mL of water. Stir magnetically at 300 rpm for 1 h to obtain a dextran amino solution. Mix the dextran amino solution with the epigallocatechin gallate complex solution from step S1 at a volume ratio of 4:1. Then add 8 mL of 10 mmol / L genipin ethanol aqueous solution (ethanol 10% v / v). Stir magnetically at 25 °C and 200 rpm for 30 min. After that, let it stand at 37 °C to form a hydrogel. Incubate overnight at 4 °C. Then wash three times with PBS buffer (pH=7.4), freeze dry, pulverize and pass through a 100-mesh sieve for later use.

[0025] Comparative Example 3 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine includes the following steps: Step 1: Weigh out the following components by weight percentage: 0.5% ectoine, 0.5% epigallocatechin gallate complex hydrogel, 2.5% lactic acid bacteria fermentation filtrate, 0.08% tea tree oil, 0.1% decyl glucoside, 0.05% purslane extract, 0.04% asiaticoside, and 96.23% water. Mix these components to prepare a liquid composition. The pH value of the liquid composition is 4.2. Step 2: Soak the sterilized and dried spunlace nonwoven fabric in the above liquid composition, wherein the mass ratio of spunlace nonwoven fabric to liquid composition is 1:3. Then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

[0026] The preparation method of the epigallocatechin gallate composite hydrogel includes the following steps: S1. Add 10g of lysozyme to 90mL of water and mix. Adjust the pH to 2.0 with 6mol / L HCl aqueous solution, and then make up to 100mL with water to obtain a lysozyme solution. Heat the lysozyme solution at 77℃ for 10h, then centrifuge at 12000rpm at 4℃ for 20min, collect the precipitate, and mix the precipitate with 10mmol / L Bis-Tris buffer (pH=5.8) and homogenize at 8000rpm for 5min to obtain a solution with a precipitate content of 5wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 10mmol / L Bis-Tris buffer (pH=5.8) to prepare a 1.0wt% epigallocatechin gallate solution. Then mix the epigallocatechin gallate solution and the lysozyme amyloid cellulose solution in equal volumes and stir for 30min to form a solution containing epigallocatechin gallate complex. S2. Weigh 2.0 g of dried porcine type I collagen and add it to 90 mL of pre-cooled 0.1 mol / L acetic acid at 4°C. Stir magnetically at 100 rpm for 2 h, then bring the volume to 100 mL with 0.1 mol / L acetic acid to obtain a collagen solution. Mix the collagen solution with the epigallocatechin gallate complex solution from step S1 at a volume ratio of 4:1, then add 8 mL of 10 mmol / L genipin ethanol aqueous solution (10% v / v ethanol). Stir magnetically at 25°C and 200 rpm for 30 min. After that, let it stand at 37°C to form a hydrogel, incubate overnight at 4°C, then wash three times with PBS buffer (pH=7.4), freeze-dry, pulverize and pass through a 100-mesh sieve for later use.

[0027] Comparative Example 4 A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine includes the following steps: Step 1: Weigh out the following components by weight percentage: 1% loaded ectoine composite hydrogel, 0.08% tea tree oil, 0.1% decyl glucoside, 0.05% purslane extract, 0.04% asiaticoside, and 98.73% water. Mix the components to prepare a liquid composition. The pH value of the liquid composition is 4.2. Step 2: Soak the sterilized and dried spunlace nonwoven fabric in the above liquid composition, wherein the mass ratio of spunlace nonwoven fabric to liquid composition is 1:3. Then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

[0028] The preparation method of the loaded ectoine composite hydrogel is the same as that in Example 3. Test Example 1

[0029] Antibacterial test According to GB 15979-2024 Hygienic Requirements for Disposable Sanitary Products, the antibacterial and soothing feminine wipes containing ectoine prepared in Examples 1-3 and Comparative Examples 1-4 of this invention were tested for antibacterial properties. The contact time was 5 minutes. The test strains were Escherichia coli (commercially available, ATCC 25922), Staphylococcus aureus (commercially available, ATCC 6538), and Candida albicans (commercially available, ATCC 10231). The specific test results are shown in Table 1 below.

[0030] Table 1

[0031] As shown in Table 1, the antibacterial rates of all examples and comparative examples were higher than 90%, indicating good antibacterial effects. Comparing Examples 1-3 and Comparative Examples 1-4, the antibacterial rates of Examples 2-3 and Comparative Examples 1-4 were higher than that of Example 1. Specifically, Example 3 achieved an antibacterial rate of 99.5% against Escherichia coli, 99.9% against Staphylococcus aureus, and 99.0% against Candida albicans, demonstrating excellent antibacterial effects. This indicates that the introduction of ectoine-loaded composite hydrogel is beneficial in increasing the antibacterial properties of feminine hygiene antibacterial and soothing wipes. The reason for this may be that epigallocatechin gallate was introduced during the preparation of the ectoine-loaded composite hydrogel, which can compensate for the deficiency of tea tree oil against Gram-negative bacteria, forming a more comprehensive antibacterial spectrum. Simultaneously, the sustained-release properties of the hydrogel can prolong the action time of the antibacterial components, thus exhibiting stronger antibacterial activity. Test Example 2

[0032] Stability test Stability tests were conducted on the feminine hygiene antibacterial and soothing wipes containing ectoine prepared according to the present invention. Twelve wipes prepared in Examples 1-3 and Comparative Examples 1-4 were selected as samples (the samples were wipes prepared from the same production batch) and randomly divided into two equal groups of six samples each, designated as the initial group and the accelerated test group. The initial group samples were immediately analyzed by HPLC to determine the ectoine content, recorded as C0 (mg / g). The accelerated test group samples were stored in a constant temperature and humidity incubator at 40℃±2℃ and 75%±5%RH for 3 months, and then analyzed by HPLC to determine the ectoine content, recorded as C1 (mg / g). The ectoine activity retention rate (%) was calculated based on the determined C0 and C1 values, using the following formula: Ectocin activity retention rate (%) = C1 / C0 × 100% The test results are averaged, as shown in Table 2 below.

[0033] Table 2

[0034] As shown in Table 2, the ectoine activity retention rates of all examples and comparative examples were higher than 90%, indicating good stability. Comparing Examples 1-3 and Comparative Examples 1-4, it was found that the ectoine activity retention rates of Examples 2-3 and Comparative Example 4 were higher than those of Examples 1 and Comparative Examples 1-3, with Example 3 exhibiting the highest ectoine activity retention rate at 99.3%. This may be because the preparation of the ectoine-loaded composite hydrogel involved the introduction of dextran amino groups and a complex containing epigallocatechin gallate, which reacted to form a highly dense and uniform three-dimensional network. Ectoine was strictly confined within the small pores, resulting in an extremely low diffusion coefficient. Simultaneously, the dextran amino groups interacted strongly with ectoine, further immobilizing it and significantly reducing its migration ability. Therefore, even in accelerated testing, ectoine maintained a high retention rate. Compared to Example 3, the collagen in the ectoine-loaded composite hydrogel of Example 2 is less stable than that of the dextran amino group, and the three-dimensional network formed by the reaction with the complex containing epigallocatechin gallate has relatively larger pores. During the accelerated test, the diffusion of ectoine is faster, so the retention rate is lower than that of Example 3. The chitosan in Comparative Example 1 has poor stability under accelerated test conditions. Due to the loose network and weak interaction, the fixation of ectoine is not firm, so the retention rate is low.

[0035] Compared with Example 3, the ectoine in Example 1 and Comparative Examples 2-3 lacked hydrogel loading protection, making it easy to migrate and be lost through diffusion during long-term storage. Furthermore, it was easily oxidized and degraded in high temperature and high humidity environments, resulting in loss of activity and thus low retention rate. Test Example 3

[0036] Vaginal mucosal repair test Vaginal mucosal tissue samples were collected from the vaginas of female New Zealand white rabbits, cut into 4 mm wide strips, and digested with 40 times the volume of 0.25% trypsin digestion solution containing 1 mg / mL collagenase. After digestion at 37°C for 45 min, mucosal epithelial cells were isolated. The mucosal epithelial cells were seeded in DMEM / F12 medium containing 10% (v / v) fetal bovine serum and cultured at 37°C and 5% CO2. The culture medium was changed every 48 hours. After 6 days, mucosal epithelial cells with good growth status were obtained. Then, the liquid composition prepared in step one of the preparation methods of Examples 1-3 and Comparative Examples 1-4 was added to the above-mentioned DMEM / F12 medium containing 10% (v / v) fetal bovine serum at a ratio of 0.2 g / mL. After extraction at 37°C for 48 h, the mixture was centrifuged at 4000 rpm for 10 min, and the supernatant was collected and recorded as the test solution of Examples 1-3 and Comparative Examples 1-4, respectively.

[0037] Add 100 μL of a solution with a density of 10 to each well of a 96-well plate. 4A suspension of mucosal epithelial cells per mL was cultured at 37°C and 5% CO2 for 24 h. The original culture medium was discarded, and then 100 μL of DMEM / F12 medium containing 10% (v / v) fetal bovine serum was added to each well (as the control group), the test solutions of Examples 1-3 (as the corresponding experimental groups in the examples), and the test solutions of Comparative Examples 1-4 (as the corresponding experimental groups in the comparative examples). The cells were then cultured at 37°C and 5% CO2 for another 72 h. Then, 5 g / L MTT solution was added to each well, and the cells were cultured for another 4 h. The liquid in the wells was discarded, and 150 μL of dimethyl sulfoxide was added. The absorbance (OD) at 570 nm for each well was measured using a multi-mode microplate reader. The relative proliferation rate (RGR) of the mucosal epithelial cells was calculated based on the measured OD of the control group and each experimental group. The specific calculation formula is as follows: Relative growth rate (RGR) (%) = OD of experimental group / OD of control group × 100% Three parallel holes were set in each group, and the average value of the results was taken. The specific results are shown in Table 3.

[0038] Table 3 Results of the vaginal mucosal repair test

[0039] As shown in Table 3, in the vaginal mucosal repair experiment, the relative proliferation rate of mucosal epithelial cells in each example was higher than 120%, indicating a good effect on repairing the vaginal mucosa. Comparing Examples 1-3 and Comparative Examples 1-4, the relative proliferation rates of Examples 2-3 and Comparative Examples 1-3 were higher than those of Examples 1 and Comparative Example 4, with Example 2 showing the highest relative proliferation rate at 179.3%. This may be because the synergistic use of ectoine-loaded composite hydrogel and lactic acid bacteria fermentation filtrate enhances vaginal mucosal repair. The triple helix structure of collagen in the ectoine-loaded composite hydrogel introduced in Example 2 contains the RGD (arginine-glycine-aspartic acid) sequence, which can directly bind to integrin receptors on the surface of mucosal epithelial cells, triggering intracellular signaling pathways and promoting cell adhesion, migration, and proliferation. Simultaneously, the dense network of pores formed by the composite hydrogel slowly releases ectoine, continuously activating cellular stress protection pathways and reducing oxidative damage, thus significantly improving the relative proliferation rate of vaginal mucosal epithelial cells. Compared to collagen-loaded ectoine composite hydrogels, the dextran amino group in Example 3 lacks the RGD sequence and cannot activate the integrin signaling pathway, thus its proliferation-promoting effect is weaker than that of collagen, and its relative proliferation rate is lower than that of Example 2. In contrast, the network formed by the chitosan-loaded ectoine composite hydrogel in Comparative Example 1 is loose, with large pores that allow ectoine to be released rapidly in the early stage of culture, but the concentration is insufficient in the later stage, and it cannot continuously protect the cells. Therefore, the relative proliferation rate of mucosal epithelial cells is low.

Claims

1. A feminine hygiene antibacterial and soothing wipe containing ectoine, characterized in that, The product comprises a substrate and a liquid composition, wherein the liquid composition comprises, by weight percentage: 0.1%-1% ectoine or ectoine-loaded composite hydrogel, 1%-3% lactic acid bacteria fermentation filtrate, 0.05%-0.1% tea tree oil, 0.06-0.12% decyl glucoside, 0.03-0.08% purslane extract, 0.02-0.05% asiaticoside, and the remainder being water.

2. The feminine hygiene antibacterial and soothing wipes containing ectoine according to claim 1, characterized in that: The substrate is spunlace nonwoven fabric.

3. The feminine hygiene antibacterial and soothing wipes containing ectoine according to claim 1, characterized in that: The pH value of the liquid composition is 3.8-4.

5.

4. The feminine hygiene antibacterial and soothing wipes containing ectoine according to claim 1, characterized in that: The mass ratio of the substrate to the liquid composition is 1:2-4.

5. The feminine hygiene antibacterial and soothing wipes containing ectoine according to claim 1, characterized in that, The preparation method of the ectoin-loaded composite hydrogel includes the following steps: S1. Add 9.5-10.5g of lysozyme to 85-95mL of water and mix. Adjust the pH to 2-2.2 with 3-6mol / L HCl aqueous solution and bring to a final volume to obtain a lysozyme solution. Then heat the lysozyme solution at 75-78℃ for 8-12h, followed by centrifugation at 10000-13000 rpm for 15-25 min at 1-5℃. Collect the precipitate, mix the precipitate with 8-12mmol / L Bis-Tris buffer, and homogenize at 7000-10000 rpm for 3-6 min to obtain a solution with a precipitate content of 4-6wt%, which is the lysozyme amyloid cellulose solution. Dissolve epigallocatechin gallate in 8-12mmol / L... Prepare a 0.5-2 wt% epigallocatechin gallate solution in Bis-Tris buffer; then mix the epigallocatechin gallate solution with an equal volume of lysozyme amyloid cellulose solution and stir for 20-30 min to form a solution containing epigallocatechin gallate complex. S2. Add ectoine powder to water to prepare an ectoine solution with a concentration of 8-12% w / v; weigh 1.8-2.2g of dried porcine type I collagen and add it to 85-95mL of pre-cooled 0.1-0.3mol / L acetic acid at 1-5℃, stir magnetically at 50-200rpm for 1-2h, and bring to a final volume to obtain a collagen solution; then mix the ectoine solution and collagen solution prepared above at a volume ratio of 1:8-10, and stir magnetically at 23-27℃ and 100-200rpm for 20-30min to obtain an ectoine-loaded collagen solution; mix the ectoine-loaded collagen solution with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 3-5:1, then add 8-9mL of 9-10mmol / L genipin ethanol aqueous solution, and stir magnetically at 23-27℃ and 100-200rpm for 20-30min to obtain an ectoine-loaded collagen solution; mix the ectoine-loaded collagen solution with the epigallocatechin gallate complex solution from step S1 above at a volume ratio of 3-5:1, then add 8-9mL of 9-10mmol / L genipin ethanol aqueous solution, and stir magnetically at 23-27℃ and 100-200rpm for 20-30min. Stir magnetically at rpm for 20-30 minutes; then let stand at 35-38℃ to form a hydrogel, incubate overnight at 3-6℃, then rinse, freeze dry, pulverize and sieve for later use.

6. The feminine hygiene antibacterial and soothing wipes containing ectoine according to claim 5, characterized in that: Step S2 can also be: Ectoin powder was added to water to prepare an ectoin solution with a concentration of 8-12% w / v. 1.8-2.2 g of dextran amino acid was weighed and added to 95-105 mL of water, and the mixture was magnetically stirred at 200-300 rpm for 0.5-1.5 h to obtain a dextran amino acid solution. The ectoin solution and the dextran amino acid solution were then mixed at a volume ratio of 1:8-10, and magnetically stirred at 23-27℃ and 100-200 rpm for 20-30 min to obtain an ectoin-loaded dextran amino solution. The ectoin-loaded dextran amino solution was mixed with the epigallocatechin gallate complex solution from step S1 at a volume ratio of 3-5:1, and then 8-9 mL of a 9-10 mmol / L genipin ethanol aqueous solution was added. The mixture was stirred at 23-27℃ and 100-200 rpm for 20-30 min to obtain an ectoin-loaded dextran amino solution. Stir magnetically at rpm for 20-30 minutes; then let stand at 35-38℃ to form a hydrogel, incubate overnight at 3-6℃, then rinse, freeze dry, pulverize and sieve for later use.

7. A method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine as described in any one of claims 1-6, characterized in that, Includes the following steps: Step 1: Weigh out ectoine, lactic acid bacteria fermentation filtrate, tea tree oil, decyl glucoside, purslane extract, asiaticoside, and water, then mix the substances to prepare a liquid composition. Step 2: Immerse the sterilized and dried substrate in the above liquid composition, then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

8. The method for preparing feminine hygiene antibacterial and soothing wipes containing ectoine as described in claim 7, characterized in that, Includes the following steps: Step 1: Weigh out the following ingredients: ectoin-loaded composite hydrogel, lactic acid bacteria fermentation filtrate, tea tree oil, decyl glucoside, purslane extract, asiaticoside, and water. Then mix the ingredients to prepare a liquid composition. Step 2: Immerse the sterilized and dried substrate in the above liquid composition, then fold and seal the package to obtain feminine care antibacterial soothing wipes containing ectoine.

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