Skin care composition and application thereof

Abstract

The invention provides a skin care composition and application thereof, and relates to the technical field of skin care. According to the present invention, it is found for the first time that the skin barrier function can be effectively improved by using the Ectoine and the Pyrus ussuriensis fruit fermentation broth in the combined manner; furthermore, by combining Ectoin with Pyrus ussuriensis fruit fermentation liquor for use, damage caused by oxygen free radicals can be effectively reduced, through the effects of the multiple aspects, the health degree of skin can be effectively improved, and the problems of facial redness and the like caused by skin barrier damage are solved.

Description

Technical Field

[0001] This application relates to the field of skin care technology, and more particularly to a skin care composition and its application. Background Technology

[0002] The skin, especially the epidermis, as the body's barrier organ, is constantly exposed to external factors in daily life, such as ultraviolet radiation, air pollution, and excessive cleaning. This leads to damage to the skin barrier, an increase in free radicals, and consequently, skin aging and varying degrees of skin discomfort (such as dryness, redness, stinging, and itching). Although various coping strategies have been adopted to combat external stimuli, such as relieving burning pain and itching by inhibiting the expression level of capsaicin receptors (TRPV1), it is still necessary to provide effective removal of substances that cause skin damage and enhance the skin barrier function to improve skin health. Summary of the Invention

[0003] Therefore, after extensive research, the inventors of this application unexpectedly discovered that the Qiuzi pear ( Ussuri pear When fruit fermentation broth and ectoine are combined in a certain proportion, they produce a synergistic effect in improving skin barrier function, thereby effectively improving skin health.

[0004] On one hand, this application provides a skin care composition comprising, in a physiologically acceptable medium, ectoine and pear (…). Ussuri pear Fruit fermentation liquid, The ectoine and Qiu Zi pear ( Ussuri pear The mass ratio of fruit fermentation liquid is (0.008-1.5):1.

[0005] Among them, Ikdoin and Qiu Zili ( Ussuri pear The mass ratio of the fruit fermentation broth can be any value or range of 0.008:1, 0.009:1, 0.01:1, 0.02:1, 0.025:1, 0.03:1, 0.04:1, 0.05:1, 0.06:1, 0.07:1, 0.08:1, 0.09:1, 0.1:1, 0.2:1, 0.3:1, 0.4:1, 0.5:1, 0.6:1, 0.7:1, 0.8:1, 0.9:1, 1:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, or 1.5:1.

[0006] Furthermore, the ectoine and the autumn pear ( Ussuri pear The mass ratio of fruit fermentation liquid is (0.01-1):1.

[0007] Furthermore, the aforementioned autumn pear ( Ussuri pear The fruit fermentation broth is the fermentation filtrate of pear fruit.

[0008] From a botanical taxonomy perspective, pears primarily belong to the genus *Pyrus* within the subfamily Maloideae of the family Rosaceae. There are numerous varieties of pears, mainly categorized into the white pear system, the sand pear system, and the autumn pear system. Pyrus ussuriensis Maxim It includes four major varieties: ) and the European pear system.

[0009] Pear, also known as peeled pear, is a species of pear in the genus Pyrus of the family Rosaceae. Pear Maxim of Ussuri Ginkgo biloba is a locally cultivated variety of pear, found in the cold, damp mountainous areas of Linxia Prefecture, Gansu Province, at an altitude of 1800-2500 meters. It is rich in free acids, pectin, polysaccharides, fats, proteins, cellulose, iron, calcium, phosphorus, and other nutrients, earning it the reputation of "the king of ginkgo nuts." Due to its warm nature and sweet, slightly sour taste, it possesses properties such as clearing heat and resolving phlegm, nourishing the stomach and lungs, quenching thirst and relieving coughs, softening blood vessels, and alleviating hangovers. It is a green food rich in nutrients.

[0010] Furthermore, the aforementioned autumn pear ( Ussuri pear The fruit fermentation broth was obtained by fermenting autumn pear fruit with grape juice containing Hansenula polysaccharide.

[0011] Preferably, the autumn pear ( Ussuri pear The fruit fermentation broth was obtained by fermenting grape juice with Hansenula polymorpha yeast to obtain pear juice.

[0012] Preferably, the grape juice Hansenula polymorpha is grape juice Hansenula polymorpha ZS02.

[0013] Grape juice contains Hansenula polymorpha ( Hanseniaspora grapes ZS02 was deposited on November 7, 2022, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCCNO.26056. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. This strain has been recorded in Chinese patent CN117165459A.

[0014] Preferably, the fermentation includes fermentation at a temperature of 25℃-40℃ and a rotation speed of 100-300 rpm for 40-60 h.

[0015] Specifically, the fermentation temperature can be any value or range from 25℃, 26℃, 27℃, 28℃, 29℃, 30℃, 31℃, 32℃, 33℃, 34℃, 35℃, 36℃, 37℃, 38℃, 39℃, and 40℃; the fermentation speed can be any value or range from 100 rpm, 110 rpm, 120 rpm, 130 rpm, 140 rpm, 150 rpm, 160 rpm, 170 rpm, 180 rpm, 190 rpm, 200 rpm, 210 rpm, 220 rpm, 230 rpm, 240 rpm, 250 rpm, 260 rpm, 270 rpm, 280 rpm, 290 rpm, and 300 rpm; and the fermentation time can be 40 h, 41 h, 42 h, 43 h, 44 h, and 45 h. h, 46h, 47h, 48h, 49h, 50h, 51h, 52h, 53h, 54h, 55h, 56h, 57h, 58h, 59h, 60h, and any time value or range thereof.

[0016] In a preferred embodiment, the pear fermentation filtrate can be prepared by referring to the method described in Chinese Patent CN117165459A.

[0017] In a preferred embodiment, the method for preparing the pear fermentation filtrate includes: Step 1: Crush and filter the pears to obtain pear juice; Step 2: Sterilize the original juice of pears to obtain the fermentation medium; Step 3: Add Hansenula polymorpha yeast to the grape juice. Hanseniaspora grapes ZS02 was inoculated into the fermentation medium and fermented for 40-60 h at 25℃-40℃ and 100-300 rpm to obtain pear fermentation filtrate.

[0018] Preferably, the preparation method may further include the steps of filtering and removing impurities from the pear fermentation filtrate.

[0019] In a preferred embodiment, the method for preparing the pear fermentation product filtrate includes: 1) Crush the ripe pears, filter them with gauze to obtain the juice, and then filter them sequentially through a 2.0 μm clarifying plate, a 0.8 μm clarifying plate, and a 0.22 μm filter membrane to obtain the original pear juice; 2) Sterilize the original juice of pear fruit at 100℃ for 10 min to obtain pear fruit juice filtrate; 3) Add Hansenula polymorpha yeast to the grape juice ( Hanseniaspora grapesA single colony of ZS02 was inoculated into the pear juice filtrate and cultured in a shake flask at 200 rpm and 30℃ for 48 h to obtain the pear fermentation filtrate. 4) Centrifuge the pear fermentation filtrate at 6000 rpm for 10 min, filter to remove bacteria, take the supernatant and sterilize at 80℃ for 1 h, then filter with a 0.22 μm filter membrane to finally obtain the pear fermentation product filtrate.

[0020] Preferably, the autumn pear ( Ussuri pear The fruit fermentation broth is the filtrate of grape juice fermented with Hansenula polymorpha / Pyrus pyrifolia.

[0021] Those skilled in the art may also choose to purchase grape juice containing Hansenula polymorpha / autumn pear prepared by Bloomage Biotechnology Co., Ltd. Ussuri pear The experiment was conducted using the filtrate of the fruit fermentation product. This application does not specify the source of the product.

[0022] Furthermore, the physiologically acceptable medium includes one or more of solvents, pH adjusters, preservatives, and thickeners.

[0023] This application may also include other functional ingredients that have no negative impact on skin repair.

[0024] This application may also add physiologically acceptable excipients, which may be suitable propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, flow aids, flavoring agents, suspending agents, coating materials, fragrances, anti-adhesion agents, binding agents, penetration enhancers, buffers, plasticizers, surfactants, foaming agents, defoamers, encapsulating agents, humectants, absorbents, diluents, flocculants and anti-flocculation agents, filter aids, release inhibitors, etc.

[0025] The compositions of this application can be prepared by general methods, wherein one or more physiologically acceptable media or carriers may be added. Those skilled in the art can select the media or carrier according to actual needs.

[0026] One aspect of this application relates to compositions, which are any compositions capable of achieving the effects described in this application. The compositions include, but are not limited to, the simultaneous or sequential use of the components. "Simultaneous use" includes using them together in the same formulation or separately in different formulations. "Sequential use" includes using them sequentially in different formulations, with no restriction on the order of sequential use.

[0027] In a preferred embodiment, the method for preparing the composition includes: mixing ectoine and pear (… Ussuri pear The fruit fermentation broth was mixed in a physiologically acceptable medium.

[0028] On the other hand, this application also provides the use of the described composition in the preparation of cosmetic products.

[0029] On the other hand, this application also provides the use of the described composition in the preparation of products that improve skin barrier function.

[0030] Since the composition of this application has the effect of improving the skin barrier, it can enhance the skin's ability to resist the invasion of harmful external factors.

[0031] On the other hand, this application also provides the use of the described composition in the preparation of products that enhance the skin's resistance to harmful external factors.

[0032] Specifically, the external harmful factors include physical, chemical, and biological harmful factors affecting the skin; more specifically, the external harmful factors include ultraviolet radiation.

[0033] On the other hand, this application also provides the use of the described composition in the preparation of products that improve skin redness.

[0034] On the other hand, this application also provides a beauty product comprising the aforementioned composition.

[0035] Furthermore, the beauty product is a topical skin product.

[0036] Preferably, the product or composition is mild and non-irritating to the human body and meets the requirements of cosmetic safety technical specifications.

[0037] Furthermore, the ectoine accounts for 0.001%-5% of the total product mass by weight. The ectoine may account for any value or range of 0.001%, 0.002%, 0.003%, 0.004%, 0.005%, 0.006%, 0.007%, 0.008%, 0.009%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, or 5% of the total product mass.

[0038] Furthermore, by weight percentage, the autumn pear ( Ussuri pear The fruit fermentation broth accounts for 0.1%-50% of the total product mass. The aforementioned Qiuzi pear ( Ussuri pearThe fruit fermentation broth can account for any value or range of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 20%, 30%, 40%, or 50% of the total product mass.

[0039] Those skilled in the art can adjust the concentration of the composition or product used in this application according to the actual situation, and no specific limitation is made in this application.

[0040] The present invention has the following beneficial effects: This application contains ectoine and pear (Akiko) Ussuri pear The composition of fruit fermentation broth can synergistically inhibit the production of reactive oxygen free radicals and reduce or eliminate the damaging effects of ultraviolet rays on the skin barrier.

[0041] Furthermore, the ectoine and Akiko pear provided in this application ( Ussuri pear The composition of fruit fermentation liquid can synergistically reduce transdermal water loss and effectively improve the skin barrier function.

[0042] Furthermore, the ectoine and Akiko pear provided in this application ( Ussuri pear The composition of fruit fermentation liquid effectively improves skin health and alleviates problems such as facial redness caused by damage to the skin barrier. Attached Figure Description

[0043] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 Before and after comparison images for the sample treatment group. Detailed Implementation

[0044] To more clearly illustrate the overall concept of this application, a detailed description is provided below with reference to the accompanying drawings and embodiments. Numerous specific details are set forth in the following description to provide a more thorough understanding of the invention. However, it will be apparent to those skilled in the art that the invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described to avoid confusion with the invention.

[0045] Before further describing specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terminology used in the embodiments of the present invention is for describing specific embodiments and not for limiting the scope of protection of the present invention. Test methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the respective manufacturers.

[0046] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof. When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, apparatus, and materials used in the embodiments, based on the knowledge of the prior art possessed by one of ordinary skill in the art and the description of this invention, any prior art methods, apparatus, and materials similar to or equivalent to those described, apparatus, and materials in the embodiments of this invention may be used to implement the present invention.

[0047] Unless otherwise specified, in the following embodiments, reagents or instruments whose manufacturers are not indicated are all conventional products that can be purchased commercially.

[0048] Among them, the ectoine mentioned in this invention, batch number: J103230157, is from Bloomage Biotechnology Co., Ltd.; the grape juice Hansenula polysaccharide / Pyrus pyrifolia fermentation product filtrate (Pyrus pyrifolia fermentation filtrate), batch number: 20220706, is from Bloomage Biotechnology Co., Ltd.

[0049] Unless otherwise stated, the experimental methods, detection methods, and preparation methods disclosed in this invention all employ conventional techniques in the fields of microbiology, biochemistry, analytical chemistry, cell culture, cosmetics, and related fields.

[0050] In addition, the "water" mentioned in this invention includes any feasible water that can be used in the art, such as deionized water, distilled water, ion-exchanged water, double-distilled water, high-purity water, and purified water.

[0051] In the following embodiments, unless otherwise specified, % means wt%, i.e., weight percentage.

[0052] Test Example 1: Repair Test (1) Preparation of test solution Alternatively, in the test substances described below, Examples 1-8 and Comparative Examples 1-2 contain ectoine and grape juice Hansenula polymorpha / autumn pear fruit ferment filtrate by weight percentage.

[0053] Composition group: Prepared according to the contents in Table 1, add ectoin and grape juice Hansenula polymorpha / autumn pear fruit fermentation product filtrate to serum-free culture medium (DMEM medium), mix and stir until completely dissolved to obtain composition sample solution, filter sterilize with 0.22 μm filter membrane to obtain the compositions of Examples 1-8 and Comparative Examples 1-2.

[0054] Grape juice Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate group: The grape juice Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate was added to serum-free culture medium, mixed and stirred until completely dissolved to obtain sample solutions of different concentrations (1%, 2%), which were then filtered through a 0.22 μm filter membrane for sterilization.

[0055] Ectoin group: Ectoin was added to serum-free culture medium, mixed and stirred until completely dissolved, to obtain sample solutions of different concentrations (0.005%, 0.01%, 0.03%, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 2%), which were then filtered through a 0.22 μm filter membrane for sterilization.

[0056] (2) Test Human epidermal keratinocytes (HaCaT) were irradiated with UVA and UVB to stimulate the production of reactive oxygen species (ROS), simulating the damage of ultraviolet radiation to the epidermal barrier. After irradiation, the cells were brought into contact with the sample, and the intracellular ROS were labeled with the fluorescent probe DCFH-DA. The intracellular ROS content was detected by flow cytometry to investigate whether the test substance could reduce or eliminate the damage of ultraviolet radiation to the skin barrier.

[0057] The specific experimental method is as follows: HaCaT cells in the logarithmic growth phase were harvested at a concentration of 1×10⁻⁶. 5 Cells were seeded at a density of 1 / mL in 12-well culture plates, with 1 mL of cell suspension in each well, and cultured in a CO2 incubator at 37°C and 5% CO2 for 24 h.

[0058] Irradiation groups (experimental group and model group) were covered with plastic wrap, and UVA was applied at 2000 μW / cm². 2 Irradiation intensity for 1 hour, UVB at 700 μW / cm 2 The irradiation intensity was 3 minutes, while the negative control group was covered with aluminum foil and not irradiated.

[0059] After irradiation, the old culture medium was discarded. The experimental group was added with the test solution, while the model group and negative control group were added with serum-free culture medium, 1 mL per well. After culturing for 24 h, all culture medium was discarded, and the cells were washed twice with PBS. 1.5 mL of DCFH-DA (dichlorofluorescein diacetate, prepared by diluting 0.375 μL DCFH-DA to 1 mL PBS) was added to each well, and the cells were incubated in a cell culture incubator for 30 min, mixing every 5 min to ensure complete probe binding. The probes were discarded, and the cells were washed twice with preheated serum-free culture medium. 1 mL of serum-free culture medium was added to each well, and the cells were incubated at 37°C for 10 min. After washing once with PBS, the cells were digested with trypsin, washed twice with PBS, resuspended in 300 μL PBS, and detected by flow cytometry. The average fluorescence intensity was recorded, and the results are shown in Table 2. Significance and synergy analyses were performed on the experimental data in Table 2.

[0060] Experimental data were obtained using the King's formula. The synergy between the two was determined, where q represents the following: 0.85-1.15 is simple addition; >1.15-20 is enhancement; >20 is significant enhancement; <0.85-0.55 is antagonism; and <0.55 is significant antagonism.

[0061] Table 1. Proportions of each embodiment and comparative example

[0062] Table 2. ROS inhibition rate and synergistic effect results

[0063] Note: The inhibition rate of the model group is 0; * indicates p < 0.05 compared with the model group; ** indicates p < 0.01 compared with the model group; *** indicates p < 0.001 compared with the model group. The grape juice Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate group used grape juice Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate with the same concentration as described in the corresponding examples or comparative examples as the test solution; the ectoine group used ectoine with the same concentration as described in the corresponding examples or comparative examples as the test solution; and the composition group used the compositions described in Examples 1-8 and Comparative Examples 1-2 as the test solution.

[0064] Table 2 shows that when ectoine and grape juice Hansenula polysaccharide / Pyrus pyrifolia fermentation product filtrate are combined in the proportions specified in Examples 1-8, they can synergistically inhibit the production of reactive oxygen free radicals, thereby reducing or eliminating the damaging effects of ultraviolet radiation on the skin barrier.

[0065] Test Example 2: Patch Test In this test case, the patch test was conducted in accordance with the "Human Skin Patch Test" in the Cosmetic Safety Technical Specifications (2015 Edition).

[0066] Instrumentation: Patch tester (Chemotechnique Diagnostics AB, Sweden).

[0067] Sample preparation: Weigh 0.2% xanthan gum and dissolve it in purified water according to the contents in Table 3. Add ectoine and the filtrate of *Hansenula polymorpha* / *Pyrus pyrifolia* fermentation product from grape juice to the solution, mix and stir until completely dissolved to obtain a sample solution with a certain viscosity. Use purified water as a control.

[0068] Subjects: 30 healthy subjects will be recruited, regardless of gender.

[0069] Test method: Tear open the packaging of the plaque applicator and measure 0.020 g of sample into the small chamber. Apply the plaque applicator to the flexor side of the subject's forearm and gently press it with the palm of your hand to make it evenly adhere to the skin for 24 hours.

[0070] The reaction results were observed and recorded according to Table 4 48 h after the spot tester was removed. The results are shown in Table 5.

[0071] Table 3

[0072] Table 4. Grading Criteria for Skin Reactions in Closed Patch Tests

[0073] Table 5. Results of patch test

[0074] Table 5 concludes that samples 1-10 all showed negative reactions and had no potential adverse effects on the human body.

[0075] Test Example 3: Transdermal Water Loss Test The transdermal water loss rate was tested using samples 1-10 of Test Example 2.

[0076] In this test case, additional samples of grape juice filtrate containing Hansenula polymorpha / Pyrus pyrifolia fermentation product and ectoine were prepared.

[0077] Grape juice Hansenula polysaccharide / Pyrus pyrifolia fermentation product filtrate sample: Weigh 0.2% xanthan gum and dissolve it in a certain amount of purified water. Then add the grape juice Hansenula polysaccharide / Pyrus pyrifolia fermentation product filtrate to the solution, mix and stir until completely dissolved to obtain sample solutions of different concentrations (1%, 2%).

[0078] Ectocin sample: Weigh 0.2% xanthan gum and dissolve it in a certain amount of purified water. Then add ectocin to the solution, mix and stir until completely dissolved to obtain sample solutions of different concentrations (0.005%, 0.01%, 0.03%, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 2%).

[0079] Use pure water as a control.

[0080] Test subjects: 30 volunteers aged 20-45.

[0081] Experimental method: A 4 cm × 4 cm area was marked on the flexor side of both forearms as the test area, and the concentration of the sample was 3.0 ± 0.1 mg / cm². 2 The sample was evenly applied to the test area using the prescribed dosage. Skin moisture loss in the test and control areas was measured before application and 3 hours after application using a Tewameter™ Hex instrument. The reduction rate of transdermal water loss was calculated from the transdermal water loss, and the synergistic effect was calculated using the formula described in Test Example 1. The test results are shown in Table 6. Transdermal water loss reduction rate = (initial value - measured value) / initial value * 100%.

[0082] Table 6. Reduction rate of transdermal water loss and synergistic effect.

[0083] Note: * indicates p < 0.05 compared to before use; ** indicates p < 0.01 compared to before use; *** indicates p < 0.001 compared to before use; The grape juice Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate sample was prepared using grape juice Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate with the same concentration as recorded in the corresponding composition sample. The ectoine group was prepared using ectoine with the same concentration as recorded in the corresponding composition sample as the sample solution. The composition sample was prepared using samples 1-10 as the sample solution.

[0084] Table 6 shows that when ectoine and grape juice Hansenula polysaccharide / Pyrus pyrifolia ferment filtrate are combined in the proportions of Examples 1-8, they can synergistically reduce transdermal water loss and effectively improve the skin barrier function.

[0085] Test Example 4: Human Skin Red Zone α Value Test (1) Sample preparation: Prepare samples and placebos according to the formulations shown in Table 7.

[0086] Table 7 Formula

[0087] (2) Experimental method: Subjects with facial redness were recruited, and a self-controlled half-face method was used.

[0088] Fifteen minutes after cleansing, subjects had images of the left and right halves of their faces taken with a VISIA CR as initial values. Then, placebo and sample were applied to the left and right sides of the face, respectively, twice a day, morning and evening. The subjects were tested again 3 days after the first use to analyze the changes in the α value of the red area.

[0089] Data Analysis: The red zone α-values ​​of images captured by VISIA CR were analyzed using IPP software. The results are shown in Table 8. Figure 1 This is a before-and-after comparison of the sample treatment group.

[0090] Table 8. Value of a in the red zone

[0091] Note: * indicates p<0.05 compared to the placebo group.

[0092] Depend on Figure 1 The results in Table 8 show that continuous use of the sample for 3 days significantly reduced the a-value in the skin red area. This indicates that the combination of ectoine and *Hansenula polymorpha* / *Pyrus pyrifolia* ferment filtrate provided in this application can effectively improve skin redness.

[0093] The above description is merely an embodiment of this application and is not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. A skin care composition characterized in that, The composition comprises in a physiologically acceptable medium: ectoine, Pyrus ussuriensis Pyrus ussuriensis ) fruit fermentation broth, The ectoine and Qiu Zi pear ( Pyrus ussuriensis The mass ratio of fruit fermentation liquid is (0.008-1.5):

1.

2. The composition according to claim 1, characterized in that, The ectoine and Qiu Zi pear ( Pyrus ussuriensis The mass ratio of fruit fermentation liquid is (0.01-1):

1.

3. The composition according to claim 1, characterized in that, The autumn pear ( Pyrus ussuriensis The fruit fermentation broth is the fermentation filtrate of pear fruit.

4. The composition according to claim 1, characterized in that, The autumn pear ( Pyrus ussuriensis The fruit fermentation broth was obtained by fermenting autumn pear fruit with grape juice containing Hansenula polysaccharide.

5. Use of a composition according to any one of claims 1 to 4 for the manufacture of a cosmetic product.

6. Use of a composition according to any one of claims 1 to 4 for the manufacture of a product for improving the barrier function of the skin.

7. Use of a composition according to any one of claims 1 to 4 for the manufacture of a product for increasing the resistance of the skin to harmful external factors.

8. A cosmetic product, characterized in that, The cosmetic product comprises a composition according to any one of claims 1 to 4.

9. The product of claim 8, wherein, The amount of said ectoine is comprised between 0.001% and 5% of the total mass of the product, in mass percentage.

10. The product of claim 8, wherein The pear juice is 0.1-50% of the total mass of the product. Pyrus ussuriensis ) fruit fermentation liquid is 0.1-50% of the total mass of the product.

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