Skin care composition containing eyebright extract

Abstract

The invention discloses a skin care composition containing an eyebright extract, which is prepared from the following raw materials: the eyebright extract, a baobab pulp extract, a rosa canina fruit extract, a safflower extract, a pomelo leaf extract, a humectant, a thickening agent, a chelating agent, a preservative, a pH regulator and deionized water. Wherein the eyebright extract is prepared by combining a high-pressure cell disruption technology and an ultrasonic extraction technology. The composition achieves the remarkable effect through the synergistic effect of multiple components, wherein the eyebright extract serves as a core active component, and experiments prove that the eyebright extract can cooperate with other plant extracts to achieve the multiple effects of preserving moisture, locking water, resisting aging, tightening skin, relieving sensitivity and the like; all the components form a stable system through optimized proportioning, and the mild and non-irritating characteristics are kept while the skin barrier function is improved.

Description

Technical Field

[0001] This invention belongs to the field of daily cosmetics technology, specifically relating to a skin care composition containing eyebright extract. Background Technology

[0002] Eyebright extract contains active compounds such as iridoids, phenylethyl glycosides, flavonoids, and phenolic acids. Flavonoids, in particular, are natural organic compounds with antioxidant, antibacterial, anti-inflammatory, hepatoprotective, and anti-cancer properties. In terms of beauty, eyebright extract can reduce eye bags, preventing the appearance of aging caused by large eye bags. It can also lighten dark circles, relieve fatigue around the eyes, and improve various signs of aging in the eye area. Furthermore, using cosmetics containing eyebright extract can prevent skin problems caused by air pollutants, inhibit the increase of inflammatory factors caused by air pollutants, repair the skin barrier, reduce redness, and enhance skin elasticity, keeping the skin healthy.

[0003] Therefore, it is of great significance to develop a new, multifunctional skincare composition that can moisturize, reduce transepidermal water loss, repair the skin barrier, reduce redness, enhance skin elasticity, and maintain healthy skin. Summary of the Invention

[0004] To address the aforementioned technical problems, the present invention aims to provide a skincare composition containing eyebright extract to overcome the shortcomings of the prior art.

[0005] To achieve the above-mentioned technical effects, the present invention adopts the following technical solution: In a first aspect, the present invention provides a skincare composition containing eyebright extract, the composition comprising the following components in weight percentage: Eyebright extract: 0.2-3.0 wt%; Baobab fruit pulp extract: 0.1-2 wt%; Rosa canis fruit extract: 0.5-5 wt%; Safflower extract: 0.01-0.2 wt%; Grapefruit leaf extract: 0.1-2.0 wt%; Moisturizer: 1-10 wt% Thickener: 0.1-2.0 wt%; Chelating agent: 0.03-0.1 wt%; Preservatives: 0.5-2 wt%; pH adjuster: 0.02-1.0 wt% Add deionized water to bring the total to 100 wt%.

[0006] Furthermore, the preparation method of the eyebright extract includes the following steps: A. Wash, dry, grind, and sieve the whole eyebright plant to obtain eyebright powder; B. Mix eyebright powder with distilled water at a ratio of 1:10-15 g / g until homogeneous, and break the mixture intermittently to obtain eyebright cell sap; wherein the breaking power is 100-150W; the breaking time is 1-2h; the breaking pressure is 1500-2500PSI; the breaking cycle is 20s breaking, 10s intermittent, and repeated. C. Extract eyebright cell sap using ultrasonic oscillation to obtain eyebright extract; the ultrasonic oscillation extraction power is 400-600W; the frequency is 30-50kHz; the temperature is 40-50℃; and the time is 30-50min. D. Centrifuge the eyebright extract at 10,000 r / min for 30 min, and collect the supernatant after centrifugation; E. Concentrate the supernatant to 10% of its original volume to obtain a concentrated solution; F. Filter the concentrated liquid, freeze-dry the concentrated filtrate to obtain eyebright extract.

[0007] Preferably, the moisturizer in the above-mentioned skin care composition includes at least one of glycerin, propylene glycol, butylene glycol, dipropylene glycol, glyceryl polyether-26, methyl gluceth-20, sorbitol, erythritol, diglyceride, polyethylene glycol-32, D-panthenol, betaine, trehalose, fructooligosaccharides, sodium polyglutamate, and sodium hyaluronate.

[0008] Polyols can be used as solvents for functional ingredients in cosmetics, as well as low-temperature stabilizers and thickeners in formulations. Glycerin is a commonly used moisturizer; it is relatively mild and has no significant irritation to the body. It has moisturizing and hydrating effects, which come not only from its hygroscopic properties but also from its ability to regulate aquaporins in the skin, control osmotic pressure balance and cell differentiation, and maintain skin hydration. In addition, active substances such as D-panthenol, sodium hyaluronate, betaine, xylitol, and trehalose can effectively penetrate into intercellular spaces and the matrix for deep hydration, further replenishing skin moisture.

[0009] Preferably, the thickener in the above-mentioned skin care composition includes at least one of acrylate / C10-30 alkanol acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, polyacryloyldimethyl taurate, xanthan gum, carbomer polymer and cellulose derivative.

[0010] Thickeners can increase the viscosity of a system, keeping it in a uniform and stable suspension or emulsion state, or forming a gel or a viscous liquid, thus improving the user experience of the product.

[0011] Preferably, the chelating agent in the above-mentioned skin care composition includes at least one of EDTA-disodium, EDTA-tetrasodium, inositol hexaphosphate, and trisodium ethylenediaminedisuccinate.

[0012] The main function of chelating agents in cosmetics is to form stable chelates with metal ions, preventing discoloration, precipitation, or other adverse reactions, thereby maintaining the stability of the cosmetics and extending their shelf life. Some chelating agents also have an auxiliary preservative effect, indirectly achieving antibacterial effects by chelating metal ions.

[0013] Preferably, the preservative in the above-mentioned skin care composition includes at least one of phenoxyethanol, 1,2-hexanediol, phenoxyethanol, sodium benzoate, and methylparaben.

[0014] Preservatives work by interfering with the enzyme systems of microorganisms, disrupting their normal metabolism, inhibiting enzyme activity, causing microbial proteins to coagulate and denature, and thus interfering with their survival and reproduction. They also alter the permeability of microbial cell membranes, leading to their inactivation. In cosmetics, preservatives prevent the formation of colonies of thermostable Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, molds, and yeasts, playing a crucial role in maintaining the stability of cosmetic quality.

[0015] Preferably, the pH adjuster in the above-mentioned skin care composition includes at least one of sodium hydroxide, potassium hydroxide, triethanolamine, citric acid, sodium citrate, and arginine.

[0016] A suitable pH level is crucial for product quality and stability. pH adjusters, as important ingredients, help maintain the ideal pH range for cosmetics. Under suitable pH conditions, cosmetics can better exert their efficacy and improve product stability and safety.

[0017] Preferably, the preparation method of the above-mentioned skin care composition containing eyebright extract includes the following steps: S1. Place the humectant, chelating agent, thickener and deionized water in a container, heat to 80-85℃, stir evenly, keep warm for 10-15 minutes to obtain mixture A; S2. Place the preservative in a container, heat to 65-70℃, stir until homogeneous, and obtain mixture B; S3. Cool mixture A to 65-70℃, add mixture B, and stir until homogeneous to obtain the mixture; S4. When the temperature of the mixture drops to 40-45℃, add eyebright extract, baobab pulp extract, rosehip extract, safflower extract, and pomelo leaf extract, stir well, and obtain mixture C; S5. Add a pH adjuster to mixture C to adjust the pH to 5.5-6.5 to obtain a skin care composition.

[0018] Secondly, the present invention also provides the use of the skin care composition containing eyebright extract described in the first aspect in the preparation of cosmetics.

[0019] Preferably, the skincare composition containing eyebright extract is present in the cosmetic at a concentration of 1-99 wt%.

[0020] Compared with the prior art, the present invention has the following beneficial effects: This invention employs high-pressure cell disruption and ultrasonic extraction technologies to break down eyebright cells, releasing the cellular matrix. Utilizing the cavitation effect of ultrasound, the effective active ingredients in eyebright are extracted efficiently, effectively preserving them and thus enhancing the efficacy of the eyebright extract. The dried eyebright extract powder is easy to store and transport, reducing costs.

[0021] Eyebright is rich in phenylpropanoid glycosides (such as verbascoside and isornascoside) and flavonoids (such as luteolin and quercetin). These active ingredients effectively inhibit the release of pro-inflammatory factors (such as TNF-α and IL-6) and reduce skin inflammation at its source by blocking inflammatory pathways such as NF-κB. Polyphenols and flavonoids are powerful natural antioxidants that effectively neutralize free radicals and prevent oxidative stress damage to skin cells. They help prevent photoaging, protect collagen and elastin, and delay the formation of fine lines and wrinkles. As part of your daytime anti-pollution skincare routine, they enhance the skin's defenses.

[0022] Baobab fruit pulp extract is rich in Vitamin C (6 times that of oranges), polyphenols, and flavonoids. These components work synergistically to effectively neutralize free radicals. It prevents and delays photoaging by reducing oxidative damage to the skin caused by environmental factors such as UV rays. It protects structural proteins by helping to protect collagen and elastin, slowing the appearance of wrinkles and sagging. Rich in natural polysaccharides and mucilage, it forms a breathable hydrating film on the skin surface, effectively preventing transepidermal moisture loss and providing long-lasting deep hydration for dry and dehydrated skin. It also contains amino acids and minerals (such as potassium, calcium, and magnesium) to help maintain the health and stability of the skin barrier, soothing dryness and tightness caused by impaired barrier function. Its polyphenols and flavonoids have natural anti-inflammatory properties, inhibiting the release of inflammatory factors. It helps soothe redness and sensitive skin and has an auxiliary effect in relieving minor skin irritation and inflammation. The high Vitamin C content is an essential cofactor for collagen synthesis, directly stimulating fibroblasts to produce more type I and type III collagen, making the skin firmer and more elastic. Combined with hyaluronic acid: enhances moisturizing effect, achieving dual protection of "hydration + moisture retention".

[0023] Rosehip extract is rich in a powerful combination of antioxidants, including Vitamin C, lycopene, beta-carotene, flavonoids, and anthocyanins. Its natural Vitamin C content far exceeds that of common citrus fruits. These components work synergistically to effectively neutralize free radicals that cause aging, preventing and repairing photoaging damage caused by environmental factors such as UV rays and pollution. It protects the skin's structural proteins (collagen and elastin), delaying the formation of wrinkles. Its anti-aging properties and ability to promote collagen production indirectly protect existing collagen from free radical degradation. It improves skin elasticity and firmness, reducing fine lines and wrinkles, making skin look fuller and younger. The abundant polyphenols and flavonoids have excellent anti-inflammatory properties, inhibiting the release of inflammatory mediators. The essential fatty acids (such as linoleic acid and alpha-linolenic acid) in its base oil help repair the skin barrier and relieve inflammation, helping to soothe redness and sensitive skin.

[0024] Grapefruit leaf extract: Soothing and anti-inflammatory, grapefruit leaves are rich in flavonoids (such as naringin and rutin) and polyphenols. These components can effectively inhibit the release of inflammatory mediators (such as histamine, TNF-α, and IL-6), thereby reducing skin inflammation at its source. Flavonoids and polyphenols are powerful natural antioxidants that can effectively neutralize free radicals and reduce oxidative stress damage to the skin. They help prevent photoaging, protect collagen and elastin, and resist environmental pollution damage to the skin.

[0025] Safflower extract contains flavonoids, which have the ability to scavenge free radicals, helping to resist environmental oxidative stress and assisting in the prevention of photoaging. Safflower extract has blood-activating, anti-inflammatory, and antioxidant effects. By improving skin blood circulation, promoting skin metabolism, scavenging free radicals, inhibiting melanin deposition, accelerating the fading of spots and discoloration, and absorbing ultraviolet rays, it achieves whitening, sun protection, and anti-aging effects. It also has significant soothing effects on contact dermatitis, seborrheic dermatitis, and neurodermatitis.

[0026] Eyebright extract, baobab pulp extract, rosehip extract, safflower extract, and grapefruit leaf extract—these five plant extracts are each rich in unique antioxidants, forming a powerful multi-dimensional antioxidant pathway. Through scientific formulation, they can create a comprehensive and synergistic skincare network of "soothing-repairing-antioxidant-strengthening," making them especially suitable for sensitive, red, and barrier-damaged skin.

[0027] Both safflower extract and Rosa canis fruit extract are excellent sources of essential fatty acids (linoleic acid and linolenic acid), which can effectively repair intercellular lipids. The fatty acids and provitamin A in Rosa canis fruit work synergistically to further promote barrier regeneration. Together, they fill barrier gaps, reduce moisture loss, and fundamentally reduce sensitivity and redness caused by barrier damage.

[0028] Both baobab fruit extract and rosehip extract are natural treasure troves of vitamin C and polyphenols. Baobab polysaccharides form a hydrophilic film on the skin's surface, trapping moisture. Rosehip lipids form a water-locking film, achieving a perfect closed loop of "endogenous hydration + exogenous moisture retention." Rosehip vitamin C stimulates collagen production, acting like new "steel bars" for the skin; while baobab's various antioxidants protect these collagens from free radical damage and provide additional nutrition. Together, they fundamentally improve skin firmness and elasticity, combating aging. They are not simply a functional superposition, but rather work synergistically across four dimensions—"soothing, repairing, nourishing, and defending"—through different targets, mutually promoting each other to push the skin towards a healthier, stronger, and more stable state. Detailed Implementation

[0029] The following embodiments are only used to illustrate the technical solutions of the present invention more clearly, and are therefore only examples and should not be used to limit the scope of protection of the present invention.

[0030] Those skilled in the art will understand that the present invention can be practiced without certain specific details. In other embodiments, methods, means, apparatus, and steps well known to those skilled in the art have not been described in detail to highlight the spirit of the invention. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Unless otherwise specified, all units used in this specification are International Standard Units (SI), and all numerical values ​​and ranges appearing in this invention should be understood to include systematic errors unavoidable in industrial production.

[0031] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the materials, reagents or instruments used, unless otherwise specified by the manufacturer, are all commercially available reagents and materials; the conditions not specified in the examples are all carried out according to conventional conditions or conditions recommended by the manufacturer; at the same time, the present invention does not limit the source of the raw materials used, unless otherwise specified, the raw materials used in the present invention are all commercially available products in this technical field.

[0032] The raw materials used in this invention and their sources are as follows: Eyebright: Purchased from Bozhou Haoyunlai Pharmaceutical Sales Co., Ltd.; Baobab fruit pulp extract: purchased from Greentech; Rosa canis fruit extract: purchased from Lipoid; Safflower extract: purchased from Xi'an Chengxiang Biotechnology Co., Ltd.; All other raw materials and reagents required for the experiment were commercially available products.

[0033] Preparation of eyebright extract Eyebright extract 1: A. Wash, dry, and grind the whole eyebright plant into powder, and sieve it through a 100-mesh sieve to remove eyebright powder smaller than 100 mesh. B. Mix eyebright powder with distilled water at a ratio of 1:15 g / g until homogeneous. Add the mixed solution to a high-pressure cell disruptor and disrupt intermittently to obtain eyebright cell sap. The disruption power is 120 W; the disruption time is 2 h; the disruption pressure is 2000 PSI; the disruption cycle is 20 s disruption followed by 10 s intervals, repeated continuously. C. Extract the eyebright cell sap from step B using ultrasonic oscillation to obtain eyebright extract; wherein the ultrasonic oscillation extraction power is 500W, the frequency is 40kHz, the temperature is 45℃, and the time is 40min. D. Place the eyebright extract obtained in step C into a centrifuge and centrifuge at a speed of 10000 r / min for 30 min. After centrifugation, take the supernatant. E. The supernatant is concentrated by vacuum evaporation at a vacuum level of 0.08 MPa and a temperature of 50°C, reducing the supernatant to 10% of its original volume to obtain a concentrated liquid. F. Extract and filter the concentrate using a microporous membrane, separate the concentrated filtrate, and freeze the concentrated filtrate into a dry powder using a freeze dryer to obtain eyebright extract.

[0034] Eyebright extract 2: A. Wash, dry, and grind the whole eyebright plant into powder, and sieve it through a 100-mesh sieve to remove eyebright powder smaller than 100 mesh. B. Mix eyebright powder with distilled water at a ratio of 1:12 g / g until homogeneous. Add the mixed solution to a high-pressure cell disruptor and disrupt intermittently to obtain eyebright cell sap. The disruption power is 150 W; the disruption time is 1.5 h; the disruption pressure is 2500 PSI; and the disruption cycle is 20 s disruption followed by 10 s intervals, repeated continuously. C. Extract the eyebright cell sap from step B using ultrasonic oscillation to obtain eyebright extract; wherein the ultrasonic oscillation extraction power is 600W; the frequency is 30kHz; the temperature is 40℃; and the time is 30min. D. Place the eyebright extract obtained in step C into a centrifuge and centrifuge at a speed of 10000 r / min for 30 min. After centrifugation, take the supernatant. E. The supernatant is concentrated by vacuum evaporation at a vacuum level of 0.08 MPa and a temperature of 50°C. The supernatant is concentrated to 10% of its original volume to obtain a concentrated liquid. F. Extract and filter the concentrate using a microporous membrane, separate the concentrated filtrate, and freeze the concentrated filtrate into a dry powder using a freeze dryer to obtain eyebright extract.

[0035] Eyebright extract 3: A. Wash, dry, and grind the whole eyebright plant into powder, and sieve it through a 100-mesh sieve to remove eyebright powder smaller than 100 mesh. B. Mix eyebright powder with distilled water at a ratio of 1:10 g / g until homogeneous. Add the mixed solution to a high-pressure cell disruptor and disrupt intermittently to obtain eyebright cell sap. The disruption power is 100W; the disruption time is 1h; the disruption pressure is 1500PSI; the disruption cycle is 20s disruption followed by 10s interval, repeated continuously. C. The eyebright cell sap from step B is subjected to ultrasonic oscillation extraction to obtain eyebright extract; wherein the ultrasonic oscillation extraction power is 400W, the frequency is 50kHz, the temperature is 50℃, and the time is 50min. D. Place the eyebright extract obtained in step C into a centrifuge and centrifuge at a speed of 10000 r / min for 30 min. After centrifugation, take the supernatant. E. The supernatant is concentrated by vacuum evaporation at a vacuum level of 0.08 MPa and a temperature of 50°C. The supernatant is concentrated to 10% of its original volume to obtain a concentrated liquid. F. Extract and filter the concentrate using a microporous membrane, separate the concentrated filtrate, and freeze the concentrated filtrate into a dry powder using a freeze dryer to obtain eyebright extract.

[0036] Eyebright extract 4: Commercially available eyebright extract, purchased from Lipoid, product number 118848.

[0037] Eyebright extract 5: The difference between this and eyebright extract 1 is that it lacks ultrasonic extraction. The specific steps are as follows: A. Wash, dry, and grind the whole eyebright plant into powder, and sieve it through a 100-mesh sieve to remove eyebright powder smaller than 100 mesh. B. Mix eyebright powder with distilled water at a ratio of 1:15 g / g until homogeneous. Add the mixed solution to a high-pressure cell disruptor and disrupt intermittently to obtain eyebright cell sap. The disruption power is 120 W; the disruption time is 2 h; the disruption pressure is 2000 PSI; and the disruption cycle is 20 s disruption followed by 10 s intervals, repeated continuously. C. Place the millet grass cell solution obtained in step B into a centrifuge and centrifuge at a speed of 10000 r / min for 30 min. After centrifugation, take the supernatant. D. The supernatant is concentrated by vacuum evaporation at a vacuum level of 0.08 MPa and a temperature of 50°C. The supernatant is concentrated to 10% of its original volume to obtain a concentrated liquid. E. Extract and filter the concentrate using a microporous membrane, separate the concentrated filtrate, and freeze the concentrated filtrate into a dry powder using a freeze dryer to obtain eyebright extract.

[0038] Eyebright extract 6: The difference between this and eyebright extract 1 is that it lacks a high-pressure cell disruptor. The specific steps are as follows: A. Wash, dry, and grind the whole eyebright plant into powder, and sieve it through a 100-mesh sieve to remove eyebright powder smaller than 100 mesh. B. Mix eyebright powder with distilled water at a ratio of 1:15 g / g until homogeneous. Extract the mixture by ultrasonic oscillation to obtain eyebright extract. The ultrasonic oscillation extraction power is 500W, the frequency is 40kHz, the temperature is 45℃, and the time is 40min. C. Place the eyebright extract obtained in step B into a centrifuge and centrifuge at a speed of 10000 r / min for 30 min. After centrifugation, take the supernatant. D. The supernatant is concentrated by vacuum evaporation at a vacuum level of 0.08 MPa and a temperature of 50°C. The supernatant is concentrated to 10% of its original volume to obtain a concentrated liquid. E. Extract and filter the concentrate using a microporous membrane, separate the concentrated filtrate, and freeze the concentrated filtrate into a dry powder using a freeze dryer to obtain eyebright extract.

[0039] Preparation of skincare compositions containing eyebright extract The specific dosage of each component in the skincare composition containing eyebright extract is shown in Table 1 below, in units of mass percentage.

[0040] Table 1. Specific dosage of each component in the skincare composition containing eyebright extract.

[0041] Note: "Appropriate amount" for phase D means that phase D is used to adjust the pH to the specified value. The "specified value" refers to the pH value specified in the preparation method steps of the embodiment. " / " indicates that no addition is required.

[0042] The preparation method of Example 1 includes the following steps: S1. Place each component of phase A in a container, heat to 80°C, stir evenly, and keep warm for 10 minutes to obtain mixture A; S2. Place each component of phase B in a container, heat to 70°C, and stir until homogeneous to obtain mixture B; S3. Cool mixture A to 70℃, add mixture B, and stir until homogeneous to obtain the mixture; S4. After the temperature of the mixture drops to 40℃, add the components of phase C, stir evenly, and obtain mixture C; S5. Add phase D to mixture C and adjust the pH to 5.5 to obtain the skin care composition.

[0043] The preparation method of Example 2 includes the following steps: S1. Place each component of phase A in a container, heat to 83°C, stir evenly, and keep warm for 15 minutes to obtain mixture A.

[0044] S2. Place each component of phase B in a container, heat to 68°C, and stir until homogeneous to obtain mixture B.

[0045] S3. Cool mixture A to 68°C, add mixture B, and stir until homogeneous to obtain the final mixture.

[0046] S4. After the temperature of the mixture drops to 43℃, add the components of phase C, stir evenly, and obtain mixture C.

[0047] S5. Add phase D to mixture C and adjust the pH to 6.0 to obtain the skin care composition.

[0048] The preparation method of Example 3 includes the following steps: S1. Place each component of phase A in a container, heat to 85°C, stir evenly, and keep warm for 13 minutes to obtain mixture A.

[0049] S2. Place each component of phase B in a container, heat to 65°C, and stir until homogeneous to obtain mixture B.

[0050] S3. Cool mixture A to 65°C, add mixture B, and stir until homogeneous to obtain the final mixture.

[0051] S4. After the temperature of the mixture drops to 45℃, add the components of phase C, stir evenly, and obtain mixture C.

[0052] S5. Add phase D to mixture C and adjust the pH to 6.5 to obtain the skin care composition.

[0053] The preparation methods and steps of Comparative Examples 1-10 are the same as those in Example 2.

[0054] The facial skincare compositions prepared in the examples and comparative examples were subjected to chicken embryo irritation tests: Test Example 1: Stimulation Test The test method refers to SN / T 2329-2009 "Cosmetic Eye Irritation / Corrosiveness Chicken Embryo Villi Allantoic Membrane Test"; Scoring criteria: Reaction time method (IS): Average score < 1 indicates no stimulus; 1 ≤ average score < 5 indicates mild stimulus; 5 ≤ average score < 10 indicates moderate stimulus; average score ≥ 10 indicates strong stimulus.

[0055] The evaluation table for the irritation test of chicken embryos is shown in Table 2 below.

[0056] Table 2 Results of Chicken Embryo Irritation Test Test Project Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Chicken embryo irritation level (average score) 0.54 0.36 0.94 2.95 1.94 2.06 1.55 1.74 1.68 1.84 1.02 1.23 1.34 As shown in Table 2, the skin care composition provided by the present invention is mild, low in irritation, and highly safe, making it suitable for use on sensitive skin.

[0057] Test Example 2: Human Efficacy Test 1. Test Objectives and Principles By using the test sample on adult subjects with sensitive skin for 14 days, and using instrumental testing methods, the efficacy of the test sample in moisturizing, repairing, improving skin elasticity, soothing, and being suitable for sensitive skin was evaluated and verified by comparing before and after the test.

[0058] 1.1 Skin Hemoglobin: Based on the principle of light reflection, a quantified hemoglobin value is obtained. A decrease in the value indicates that the skin has been soothed.

[0059] 1.2 Transepidermal water loss value: Based on Fick's law of diffusion, the water vapor partial pressure gradient at different points per unit time and unit cross-sectional area was used to obtain the transepidermal water loss value. A decrease in the value indicates that the rate of skin moisture evaporation is slowed down and the skin barrier function is improved.

[0060] 1.3 Stratum Corneum Moisture Content: The differences in the dielectric constants of water and other substances when determining the moisture content of human skin stratum corneum using the capacitance method are significant. Different measured skin capacitance values ​​can represent the skin's moisture content. An increased value indicates an increased moisture content in the stratum corneum.

[0061] 1.4 Lactic Acid Stinging Test: The mechanism of the lactic acid stinging reaction is that damage to the skin barrier increases the transepidermal permeation of lactic acid. Because the barrier does not fully protect nerve endings, a lactic acid stinging reaction occurs. Scoring is performed using the "4-point method" in Table 3, and a questionnaire is completed. Those with a cumulative lactic acid stinging score ≥3 are considered to have lactic acid stinging and are deemed to have sensitive skin, thus eligible to participate in the test.

[0062] 1.5 Skin elasticity: Based on the skin's resilience, the higher the test value, the better the skin elasticity.

[0063] Table 3 Lactic acid stinging rating scale

[0064] 2. Test Samples and Requirements Test samples: Examples 1-3, Comparative Examples 1-10.

[0065] Requirements: Before the trial, a necessary safety assessment of the test samples should be completed to ensure that the test samples will not pose any foreseeable risks to the health of the subjects under the conditions of use specified in the protocol.

[0066] 3. Subject inclusion criteria Inclusion criteria: age 18-65 years (excluding pregnant and lactating women); no serious systemic diseases, immunodeficiency or autoimmune diseases; no prior skin treatments, cosmetic procedures or other tests that may affect the results at the test site; no active allergic diseases; no sensitivity to commonly used cosmetics; no use of hormonal drugs or immunosuppressants within the past month; no highly sensitive constitution; no participation in other clinical trials at the test site in the present or past three months; able to read and understand all contents of the informed consent form and voluntarily sign the informed consent form; and a cumulative lactic acid stinging sensation score ≥3.

[0067] Restrictions During the test, hot water and spicy food are prohibited; frequent entry and exit from the constant temperature and humidity room is prohibited; and the test area must not be covered or touched during the test.

[0068] 4 Test Content 4.1 Testing Instruments Stratum corneum moisture content tester (Delfin MoistureMeterSC); transepidermal water loss meter (Delfin VaPoMeter); skin color test probe (Delfin SkinColorCatch); skin elasticity test probe (Delfin ElastiMeter); temperature / humidity recorder.

[0069] 4.2 Materials Cleansing wipes, 75% alcohol, and 10% lactic acid solution.

[0070] 4.3 Test Environment Environmental requirements: Temperature: 21.0±1.0℃; Relative humidity: 50%±10%.

[0071] 4.4 Test Time Points Within 12 hours before using the test sample; within 12 hours after the last use of the test sample on day 14.

[0072] 4.5 Testing Process 4.5.1 Before using the test sample On the first visit, the participants are given an explanation of the trial and sign an informed consent form; Before the test, the subjects sat quietly for at least 10 minutes in a laboratory with a temperature of 21±1℃ and a relative humidity of 50%±10%, without drinking water or beverages, with their faces exposed, placed in the test position, and kept relaxed. The initial values ​​of facial skin stratum corneum moisture content, transepidermal water loss, skin hemoglobin, and lactic acid stinging score were measured and recorded as 0 days.

[0073] 4.5.2 Use of test samples Apply an appropriate amount of the test sample to the facial skin and gently massage until absorbed. Use once in the morning and once in the evening.

[0074] 4.5.3 After using the test sample Samples were distributed to volunteers, who were informed of the instructions for use. On day 14, within 12 hours of sample use, facial skin stratum corneum moisture content, transepidermal water loss, skin hemoglobin, skin elasticity, and lactic acid stinging score were measured and recorded for 14 days. The average rate of change in facial skin stratum corneum moisture content, transepidermal water loss, skin hemoglobin, skin elasticity, and lactic acid stinging score were calculated using the appropriate formulas. The results are shown in Table 4 below.

[0075] ; Where X 14d The values ​​representing epidermal water loss, skin hemoglobin, skin elasticity, or lactic acid stinging score on day 14; X 0d The test value represents the epidermal water loss, skin hemoglobin, skin elasticity, or lactic acid stinging score on day 0.

[0076] Table 4 Results of Human Efficacy Tests Test Project Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Number of test participants / person 10 10 10 10 10 10 10 10 10 10 10 10 10 Average change rate of stratum corneum moisture content / % 20.4 38.2 40.5 5.6 10.3 14.8 12.2 16.5 15.2 14.3 31.2 18.3 19.1 Average change rate of transepidermal water loss / % -13.1 -18.3 -20.2 0.1 -7.3 -7.9 -7.4 -9.4 -9.5 -9.7 -14.5 -11.5 -10.6 Average change rate of skin hemoglobin / % -14.6 -22.8 -23.6 -0.8 -6.5 -6.8 -6.6 -7.1 -7.2 -7.0 -16.4 -13.7 -12.5 Average change rate of lactic acid stinging score / % -18.3 -34.1 -37.9 -0.5 -6.2 -6.9 -7.4 -10.8 -8.1 -9.4 -26.7 -17.6 -17.9 Average change rate of skin elasticity / % 11.9 18.1 20.8 2.2 6.5 8.0 7.5 6.7 8.8 8.5 13.9 9.8 10.1 As shown in Table 4, the results of comparing Examples 1-3 show that the skin care composition provided by the present invention can enhance the moisturizing effect of the skin care composition, reduce transepidermal water loss, reduce skin redness, alleviate skin redness, enhance skin barrier function and improve skin elasticity. Among them, Example 2 is the preferred embodiment, which has strong efficacy and high cost performance.

[0077] In Comparative Example 2, after 14 days of skincare composition, eyebright extract showed the effects of increasing skin moisture content, reducing transepidermal water loss, reducing redness, alleviating redness, enhancing skin barrier function, and improving skin elasticity.

[0078] As can be seen from the comparison between Example 2 and Comparative Examples 1, 2 and 3, eyebright extract has a significant synergistic effect with baobab pulp extract, rosehip extract, safflower extract and pomelo leaf extract. When used together, they can enhance the moisturizing effect of the skin care composition, reduce transepidermal water loss, reduce skin redness, alleviate skin redness, enhance skin barrier function and improve skin elasticity, and make the skin healthier.

[0079] As can be seen from the comparison between Example 2 and Comparative Examples 4-7, under the condition of the same amount of eyebright extract added, the absence of any one of the extracts of baobab pulp extract, Rosa canis fruit extract, safflower extract, and pomelo leaf extract is not as effective as the combined use of eyebright extract with baobab pulp extract, Rosa canis fruit extract, safflower extract, and pomelo leaf extract. This further verifies that there is a significant synergistic effect between the eyebright extract prepared in this invention and the baobab pulp extract, Rosa canis fruit extract, safflower extract, and pomelo leaf extract.

[0080] As can be seen from the comparison between Example 2 and Comparative Example 8, the eyebright extract prepared by the steps defined in this invention has a much better efficacy than commercially available eyebright extract.

[0081] As can be seen from the comparison of Example 2 and Comparative Examples 9 and 10, the high-pressure cell disruption technology and ultrasonic extraction technology used in the eyebright extraction process defined in this invention have a significant impact on the efficacy of eyebright extract. The two technologies complement each other; eyebright extracts lacking either technology have poor efficacy. This indicates that the combination of high-pressure cell disruption technology and ultrasonic extraction technology in this invention significantly improves the actual efficacy of eyebright extract.

[0082] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0083] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

[0084] Furthermore, various different embodiments of the present invention can be combined in any way, as long as they do not violate the spirit of the present invention, they should also be regarded as the content disclosed by the present invention.

Claims

1. A skincare composition containing eyebright extract, characterized in that, The skincare composition comprises the following components by weight percentage: Eyebright extract: 0.2-3 wt%; Baobab fruit pulp extract: 0.1-2 wt%; Rosa canis fruit extract: 0.5-5 wt%; Safflower extract: 0.01-0.2 wt%; Grapefruit leaf extract: 0.1-2.0 wt%; Moisturizer: 1-10 wt% Thickener: 0.1-2.0 wt%; Chelating agent: 0.03-0.1 wt%; Preservatives: 0.5-2 wt%; pH adjuster: 0.02-1.0 wt% Add deionized water to bring the total to 100 wt%.

2. The composition according to claim 1, characterized in that, The preparation method of the eyebright extract includes the following steps: A. Wash, dry, grind, and sieve the whole eyebright plant to obtain eyebright powder; B. Mix eyebright powder with distilled water at a ratio of 1:10-15 g / g until homogeneous, and break the mixture intermittently to obtain eyebright cell sap; wherein the breaking power is 100-150W; the breaking time is 1-2h; the breaking pressure is 1500-2500PSI; the breaking cycle is 20s breaking, 10s intermittent, and repeated. C. Extract eyebright cell sap using ultrasonic oscillation to obtain eyebright extract. The ultrasonic oscillation extraction power is 400-600W; the frequency is 30-50kHz; the temperature is 40-50℃; and the time is 30-50min. D. Centrifuge the eyebright extract at 10,000 r / min for 30 min, and collect the supernatant after centrifugation; E. Concentrate the supernatant to 10% of its original volume to obtain a concentrated solution; F. Filter the concentrated liquid, freeze-dry the concentrated filtrate to obtain eyebright extract.

3. The composition according to claim 1, characterized in that, The moisturizer includes at least one of glycerin, propylene glycol, butylene glycol, dipropylene glycol, glycerin polyether-26, methyl gluceth-20, sorbitol, erythritol, diglyceride, polyethylene glycol-32, D-panthenol, betaine, trehalose, fructooligosaccharides, sodium polyglutamate, and sodium hyaluronate.

4. The composition according to claim 1, characterized in that, The thickener includes at least one of the following: acrylate / C10-30 alkanol acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, polyacryloyldimethyl taurate, xanthan gum, carbomer polymer and cellulose derivative.

5. The composition according to claim 1, characterized in that, The chelating agent includes at least one of EDTA-disodium, EDTA-tetrasodium, inositol hexaphosphate, and trisodium ethylenediaminedisuccinate.

6. The composition according to claim 1, characterized in that, The preservatives include at least one of the following: phenoxyethanol, 1,2-hexanediol, phenoxyethanol, sodium benzoate, and methylparaben.

7. The composition according to claim 1, characterized in that, The pH adjuster includes at least one of sodium hydroxide, potassium hydroxide, triethanolamine, citric acid, sodium citrate, and arginine.

8. The composition according to any one of claims 1-7, characterized in that, The method for preparing the composition includes the following steps: S1. Place the humectant, chelating agent, thickener and deionized water in a container, heat to 80-85℃, stir evenly, keep warm for 10-15 minutes to obtain mixture A; S2. Place the preservative in a container, heat to 65-70℃, stir until homogeneous, and obtain mixture B; S3. Cool mixture A to 65-70℃, add mixture B, and stir until homogeneous to obtain the mixture; S4. When the temperature of the mixture drops to 40-45℃, add eyebright extract, baobab pulp extract, rosehip extract, safflower extract, and pomelo leaf extract, stir well, and obtain mixture C; S5. Add a pH adjuster to mixture C to adjust the pH to 5.5-6.5 to obtain a skin care composition.

9. The use of a skin care composition containing eyebright extract as described in any one of claims 1-7 in the preparation of cosmetics.

10. The application according to claim 9, characterized in that, The skincare composition containing eyebright extract is present in a cosmetic concentration of 1-99 wt%.

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