Geobacillus stearothermophilus cKM-Geo and application thereof, shrimp shell oligosaccharide peptide compound and preparation method thereof

By using thermophilic Bacillus stearothermophilus cKM-Geo fermentation coupled with steam explosion to process shrimp shells, the environmental pollution and high cost problems of chemical and enzymatic methods for preparing chitosan oligosaccharides have been solved, realizing the green and efficient preparation of shrimp shell oligosaccharide peptides suitable for intestinal regulation foods.

CN121852276APending Publication Date: 2026-04-14陈慕涵
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Patent Information

Application Number
CN202610076853.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-20
Publication Date
2026-04-14

AI Technical Summary

Technical Problem

In existing technologies, chemical methods for preparing chitosan oligosaccharides are characterized by severe environmental pollution and high costs, while enzymatic methods are costly and difficult to efficiently convert shrimp shell resources. Conventional fermentation temperatures are prone to contamination by miscellaneous bacteria, leading to increased production costs and making it difficult to achieve efficient and green preparation of shrimp shell oligosaccharide peptides.

Method used

Shrimp shells were fermented using Bacillus stearothermophilus cKM-Geo coupled with steam explosion. By utilizing the high secretion of proteolytic enzymes, cellulases, lipases, and chitin deacetylases, shrimp shell oligosaccharide peptides were prepared. This method avoids the use of acid and alkali and strict separation and purification processes, achieving green and efficient conversion.

Benefits of technology

This study achieved efficient preparation of shrimp shell oligosaccharide peptides, resulting in products with good sensory properties, long shelf life, high resource utilization, reduced wastewater discharge, and good product safety. These products are suitable for intestinal regulation foods, leading to significant economic benefits.

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Abstract

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.
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