A cutting breeding method of sea coast chionanthus

Abstract

The application provides a cutting breeding method of beach cat's tail wood, and relates to the technical field of plant cutting seedling raising. The cutting breeding method of the beach cat's tail wood is as follows: after branch of the beach cat's tail wood is treated by using NAA, IAA, tocopherol acetate, ethanol and leaf extract of Rhamnus utilis as a rooting agent, cutting management is carried out, wherein the leaf extract of Rhamnus utilis is obtained by freeze-drying, crushing, acid treatment and ethanol extraction. The application overcomes the defects of the prior art, effectively shortens the seedling raising period of the beach cat's tail wood by the cutting seedling raising mode, further promotes the rooting and survival of the cutting seedlings, and comprehensively improves the efficiency of seedling raising.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant cutting seedling, in particular to a cutting breeding method of Dolichan drone spathacea. BACKGROUND

[0002] Dolichan drone spathacea is a evergreen tree of Planchonella in Lecythidaceae, which is distributed in Hainan and Guangdong provinces. Dolichan drone spathacea has been listed in the list of key protected wild plants in Hainan province. At present, the breeding method of Dolichan drone spathacea is mainly seed breeding. The seed breeding seedling rate of Dolichan drone spathacea is about 82.22% in 6 months, and the highest growth is 40cm in 4 months under careful management, the maximum base diameter is 7mm, the overall seedling period is long, the seedling management cost is high, and the seedling environment and pest control investment is large.

[0003] Cutting seedling is a common asexual propagation method of plants, which cuts the stems, leaves and roots of plants to cultivate independent new plants. The core advantages are high propagation efficiency, strong genetic stability, simple operation and low cost. At present, there is no related report on cutting seedling of Dolichan drone spathacea. However, based on the advantages of existing plant cutting seedling, it is very important to design a cutting seedling method of Dolichan drone spathacea to shorten the seedling period of Dolichan drone spathacea and realize rapid batch production of endangered plant Dolichan drone spathacea seedlings. SUMMARY

[0004] In view of the defects of the prior art, the present application provides a cutting breeding method of Dolichan drone spathacea, which effectively shortens the seedling period of Dolichan drone spathacea by cutting seedling, and further promotes the rooting and survival of cutting seedlings, and comprehensively improves the efficiency of seedling.

[0005] To achieve the above purpose, the present application is realized by the following technical scheme:

[0006] A cutting breeding method of Dolichan drone spathacea, the cutting breeding method comprises the following steps:

[0007] S1, preparation of cutting branches: selecting 1-2 year old Dolichan drone spathacea, cutting branches with a diameter of 0.5-2.5cm and a length of 25-30cm, cutting the upper end flat, cutting the lower end obliquely, and then disinfecting to obtain cutting branches for standby;

[0008] S2, rooting agent soaking treatment: the above cuttings are placed at 10°C for 2-3 h, then the lower end 3-8 cm is immersed in the rooting agent at room temperature for 30-50 min to obtain pretreated cuttings; and the rooting agent has the following formula: NAA 200-300 mg / L+IAA 200-300 mg / L+tocopherol acetate 40-80 mg / L+ethanol 10-20 mL / L+Racemobambusine leaf extract 15-30 mg / L+water to 1 L; the Racemobambusine leaf extract is obtained by freeze-drying, crushing, acid treatment and ethanol extraction of Racemobambusine leaves;

[0009] S3, cutting treatment: the lower end of the above pretreated cuttings is cut into the disinfected cutting substrate, and clear water is poured, and daily management is carried out.

[0010] Preferably, the cutting branch in step S1 retains 0-3 leaves, and when the cutting branch is a branch containing a terminal shoot, the upper end of the branch leaves is retained.

[0011] Preferably, the disinfection method in step S1 is to immerse the branch base 2-5 cm in a potassium permanganate solution with a concentration of 0.1%-0.2% for 10-12 h, and then wash the base with water to remove residual liquid.

[0012] Preferably, the preparation method of the Racemobambusine leaf extract comprises the following steps:

[0013] S2-1, freeze-drying the fresh Racemobambusine leaves, crushing to obtain Racemobambusine leaf powder for use;

[0014] S2-2, ultrasonic treatment of the Racemobambusine leaf powder in an acetic acid solution with a pH of 4-4.5 for 20-30 min, then filtering, removing the filtrate, and washing the filter residue with water to neutral to obtain acid-treated material for use;

[0015] S2-3, adding the above acid-treated material to an 85% ethanol solution, ultrasonic extraction at 45-50°C for 15-20 min, filtering, adding the filter residue to the 85% ethanol solution again and repeating the operation 2-3 times, and freeze-drying the combined filtrate to obtain the Racemobambusine leaf extract.

[0016] Preferably, the preparation method of the rooting agent in step S2 is to disperse tocopherol acetate and Racemobambusine leaf extract into ethanol, then mix water, NAA, IAA and stir uniformly to obtain.

[0017] Preferably, the cutting substrate in step S3 is a mixture of sand, red soil, organic fertilizer and the like.

[0018] Preferably, the disinfection method of the cutting substrate in step S3 is to pour the cutting substrate with a potassium permanganate solution with a concentration of 0.1%-0.5% and then cover the film and seal for 2-4 h, and then uncover the film and air dry for 2-3 d.

[0019] Preferably, the cutting depth of the pre-treated cutting branches in step S3 is 8-10 cm.

[0020] Preferably, the daily management mode in step S3 is to keep the cutting substrate humidity between 30%-90%, the temperature between 20℃-30℃, and the light shielding rate between 20%-80%.

[0021] The present application provides a cutting breeding method of beach cat tail wood, which has the following advantages compared with the prior art:

[0022] By treating the cutting branches of beach cat tail wood with plant growth hormone compound tocopheryl acetate, laguncularia leaves extract and other components, the growth of the root part of the cutting seedlings is effectively promoted. Meanwhile, the branches are treated at low temperature before soaking treatment with the rooting agent, which further improves the activity of the branch cutting, promotes early rooting of the branches, and comprehensively improves the survival rate and rooting effect of the beach cat tail wood cutting seedlings, and shortens the seedling raising cycle of the beach cat tail wood. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The beach cat tail wood branches cut for the present application;

[0024] Figure 2 The schematic diagram of the cutting branches of each control group and experimental group in the embodiment of the present application soaked with the rooting agent;

[0025] Figure 3 The growth schematic diagram of the cutting branches of control group 1 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root part;

[0026] Figure 4 The growth schematic diagram of the cutting branches of control group 2 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root part;

[0027] Figure 5 The growth schematic diagram of the cutting branches of experimental group 1 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root part;

[0028] Figure 6 The growth schematic diagram of the cutting branches of experimental group 2 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root part;

[0029] Figure 7 The growth schematic diagram of the cutting branches of experimental group 3 in the embodiment of the present application after cutting for 30d, wherein the left side is the whole plant growth schematic diagram, and the right side is the enlarged schematic diagram of the root part;

[0030] Figure 8 Figure 1 is a growth diagram of the cuttings of the experimental group 1 in the embodiment of the present application for 30 days after cutting, wherein the left side is a diagram of the whole plant growth, and the right side is an enlarged diagram of the root growth;

[0031] Figure 9 Figure 2 is a growth diagram of the cuttings of the experimental group 2 in the embodiment of the present application for 30 days after cutting, wherein the left side is a diagram of the whole plant growth, and the right side is an enlarged diagram of the root growth;

[0032] Figure 10 Figure 3 is a growth diagram of the cuttings of the experimental group 3 in the embodiment of the present application for 30 days after cutting, wherein the left side is a diagram of the whole plant growth, and the right side is an enlarged diagram of the root growth. DETAILED DESCRIPTION

[0033] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application are described below in a clear and complete manner with reference to the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0034] Preparation of the extract of the leaves of Laggera alata

[0035] 1. Preparation of LGM-A

[0036] (1) Freeze-dry the fresh leaves of Laggera alata, crush them, and obtain leaf powder of Laggera alata for standby;

[0037] (2) Put the leaf powder of Laggera alata into an acetic acid solution with a pH of 4, and treat it with ultrasonic waves at 600 W for 20 min. Then filter the solution, remove the filtrate, and wash the filter residue with clean water until it is neutral. Thus, an acid-treated material is obtained for standby;

[0038] (3) Put the acid-treated material into 10 times the volume of an 85% ethanol solution, and treat it with ultrasonic waves at 400 W for 15 min at a temperature of 45°C. Then filter the solution, repeat the above-mentioned ultrasonic extraction step with ethanol three times, and freeze-dry the combined filtrate. Thus, an extract of the leaves of Laggera alata is obtained, which is denoted as LGM-A.

[0039] 2. Preparation of LGM-B

[0040] (1) Freeze-dry the fresh leaves of Laggera alata, crush them, and obtain leaf powder of Laggera alata for standby;

[0041] (2) Put the leaf powder of Laggera alata into 10 times the volume of an 85% ethanol solution, and treat it with ultrasonic waves at 400 W for 15 min at a temperature of 45°C. Then filter the solution, repeat the above-mentioned ultrasonic extraction step with ethanol three times, and freeze-dry the combined filtrate. Thus, an extract of the leaves of Laggera alata is obtained, which is denoted as LGM-B.

[0042] Preparation of cutting substrate:

[0043] Mix sand, red soil, organic fertilizer, etc. in equal quality, then pour a 0.5% potassium permanganate solution and mix well, cover with plastic wrap and seal for 3 hours, then uncover and air dry for 2 days to obtain the cutting substrate.

[0044] Example 1:

[0045] Cutting propagation of beach cat tail wood:

[0046] (1) Select 1-2 year old beach cat tail wood, cut branches with a diameter of 0.5-2.5 cm and a length of 25-30 cm (retain 0-3 leaves, and when the cutting branch is a top-containing branch, retain the upper tender leaves), cut the upper end flat, cut the lower end obliquely, then soak the branch base 2-5 cm in a 0.2% potassium permanganate solution for 10 hours, then rinse with water to remove excess chemicals, complete disinfection, and obtain the cutting branch for use;

[0047] (2) Place the above cutting branch at 10°C for 3 hours, then immerse the lower end 3-8 cm in a room temperature rooting agent for 40 minutes to obtain the pretreated cutting branch;

[0048] (3) Insert the lower end 8-10 cm of the above pretreated cutting branch into the disinfected cutting substrate, pour clear water, control the humidity of the cutting substrate at 30%-90%, the temperature range after cutting is 20°C-30°C, the average temperature is about 24°C, set the shading rate at 50%, and perform routine management to observe the cutting rooting situation.

[0049] Comparative Example 1:

[0050] Cutting propagation of beach cat tail wood:

[0051] The specific cutting propagation method is basically the same as that of Example 1, except for the difference in step (2):

[0052] (2) Immerse the lower end 3-8 cm of the above cutting branch in a room temperature rooting agent for 40 minutes to obtain the pretreated cutting branch;

[0053] Experimental detection:

[0054] I. Preparation of rooting agent:

[0055] Prepare different rooting agents according to the following method:

[0056] 1. Preparation of rooting agent 1:

[0057] The 60 mg of tocopherol acetate and 20 mg of LGM-A are dissolved in 15 mL of ethanol, and after being stirred sufficiently, 250 mg of NAA and 250 mg of IAA are mixed, and water is added to make up to 1 L, to obtain rooting agent 1.

[0058] 2. Preparation of rooting agent 2:

[0059] The 60 mg of tocopherol acetate and 20 mg of LGM-B are dissolved in 15 mL of ethanol, and after being stirred sufficiently, 250 mg of NAA and 250 mg of IAA are mixed, and water is added to make up to 1 L, to obtain rooting agent 2.

[0060] 3. Preparation of rooting agent 3:

[0061] The 20 mg of LGM-A is dissolved in 15 mL of ethanol, and after being stirred sufficiently, 250 mg of NAA and 250 mg of IAA are mixed, and water is added to make up to 1 L, to obtain rooting agent 3.

[0062] 4. Preparation of rooting agent 4:

[0063] The 60 mg of tocopherol acetate is dissolved in 15 mL of ethanol, and after being stirred sufficiently, 250 mg of NAA and 250 mg of IAA are mixed, and water is added to make up to 1 L, to obtain rooting agent 4.

[0064] 5. Preparation of rooting agent 5:

[0065] The 250 mg of NAA and 250 mg of IAA are mixed, and water is added to make up to 1 L, to obtain rooting agent 5.

[0066] II. Cutting rooting experiment:

[0067] Referring to Table 1 below, different cutting propagation methods and rooting agents are selected to set up different cutting experiment groups and control groups (the conditions of soaking cutting branches in rooting agents of each group are shown in Table 1) : Figure 2 Table 1

[0068]

[0069]

[0070] III. Experiment statistics:

[0071] 1. The rooting rate (rooting rate = rooted branches / all cutting branches) and survival rate (survival rate = survived branches / all cutting branches) of each group of sea cat-tail cutting seedlings at 15 d and 30 d are counted, and the specific results are shown in Table 2:

[0072] Table 2

[0073]

[0074] ​From the above table, when using clean water as the rooting agent, the rooting rate of the cuttings treated according to the method of Example 1, i.e., the cuttings were treated at low temperature and then cut (control group 1), was improved to some extent compared with the cuttings that were not treated at low temperature and then cut (control group 2), but the overall survival rate of the cuttings was reduced;

[0075] In addition, the rooting agent added with the acid-treated LGM-A (experimental group 1) had a significantly improved rooting rate at 15 d compared with the rooting agent added with the LGM-B that was not acid-treated (experimental group 2), and the overall survival rate was also higher, while the LGM-B that was not acid-treated added in the rooting agent to some extent caused the early survival rate of the cuttings to be reduced, affecting the overall cutting effect;

[0076] The experimental group 1 added with the tocopheryl acetate in the rooting agent had an improved early rooting rate and survival rate compared with the experimental group 3 without the tocopheryl acetate, comprehensively ensuring the efficiency of cutting propagation and seedling raising.

[0077] 2. After the cuttings were cut for 30 d, 5 rooted cuttings were taken from each group, the average number of roots (main roots) and the average root length were calculated, and the specific results are shown in Table 3, and the rooting conditions of the cuttings in each group are shown in Figures 3-10 .

[0078] Table 3

[0079]

[0080] From the above Table 3 and Figures 3-10 , it can be seen that the rooting agent added with the LGM-B in the experimental group 2 had a relatively large number of roots, but the average root length was significantly shortened, i.e., the use of the LGM-B might inhibit the elongation of the cutting roots, to some extent affecting the quality of the cutting seedlings, while the rooting agent added with the LGM-A and the tocopheryl acetate in the experimental group 1 could significantly ensure the number of roots and the root length compared with the other groups, comprehensively improving the quality of the cutting seedlings.

[0081] The above examples are only used to illustrate the technical solutions of the present application, but not to limit the same; although the present application has been described in detail with reference to the foregoing examples, it should be understood by those skilled in the art that the technical solutions recorded in the foregoing examples can be modified, or some technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for propagating *Cercis chinensis* by cuttings, characterized in that, The cutting propagation method includes the following steps: S1. Preparation of cuttings: Select 1-2 year old seaside cat's tail wood, cut branches with a diameter of 0.5-2.5cm and a length of 25-30cm, make a horizontal cut at the top and a slanted cut at the bottom, and then disinfect them to obtain cuttings for later use. S2. Rooting agent soaking treatment: Place the above-mentioned cuttings at 10℃ for 2-4 hours, then immerse the lower 3-8cm of the cuttings in a rooting agent at room temperature for 30-50 minutes to obtain pre-treated cuttings; the rooting agent formula is: NAA 200-300mg / L + IAA 200-300mg / L + tocopheryl acetate 40-80mg / L + ethanol 10-20mL / L + Lagerstroemia indica leaf extract 15-30mg / L + water to make up to 1L; the Lagerstroemia indica leaf extract is obtained by freeze-drying Lagerstroemia indica leaves, pulverizing them, acid-treating them, and then extracting them with ethanol, specifically including the following steps: S2-1. Freeze-dry fresh Laguan wood leaves and then pulverize them to obtain Laguan wood leaf powder for later use. S2-2. Place the powdered leaves of *Lagerstroemia indica* in an acetic acid solution with a pH of 4-4.5 and sonicate for 20-30 minutes. Then filter, remove the filtrate, and wash the filter residue with water until neutral to obtain the acid-treated material for later use. S2-3. Add the above acid-treated material to an 85% ethanol solution and extract by ultrasonication at 45-50℃ for 15-20 minutes. Filter the mixture and repeat the process 2-3 times with the filter residue in an 85% ethanol solution. Combine the filtrates and freeze-dry to obtain the Laguanmu leaf extract. S3. Cutting treatment: Insert the lower end of the pretreated cuttings into the sterilized cutting substrate, water thoroughly, and perform routine management.

2. The method for propagating *Cephalotaxus fortunei* by cuttings according to claim 1, characterized in that: In step S1, the cuttings retain 0-3 leaves, and when the cuttings are branches with a terminal shoot, the tender leaves at the top are retained.

3. The method for propagating *Cephalotaxus fortunei* by cuttings according to claim 1, characterized in that: The disinfection method in step S1 is to immerse the base of the branch (2-5cm) in a 0.1%-0.2% potassium permanganate solution for 10-12 hours, and then rinse the base with clean water to remove any residual solution.

4. The method for propagating *Cercis chinensis* by cuttings according to claim 1, characterized in that: The rooting agent in step S2 is prepared by dispersing tocopheryl acetate and Lagerstroemia indica leaf extract in ethanol, and then mixing with water, NAA, and IAA and stirring evenly.

5. The method for propagating *Cercis chinensis* by cuttings according to claim 1, characterized in that: In step S3, the cutting substrate is a mixture of sand, red soil, organic fertilizer, etc.

6. The method for propagating *Cercis chinensis* by cuttings according to claim 1, characterized in that: In step S3, the cutting substrate is disinfected by thoroughly watering it with a 0.1%-0.5% potassium permanganate solution, then covering it with a film and sealing it for 2-4 hours, followed by removing the film and airing it out for 2-3 days.

7. The method for propagating *Cercis chinensis* by cuttings according to claim 1, characterized in that: In step S3, the depth of the pre-treated cuttings is 8-10 cm.

8. The method for propagating *Cercis chinensis* by cuttings according to claim 1, characterized in that: The daily management method in step S3 is to maintain the humidity of the cutting substrate between 30% and 90%, the temperature between 20 and 30°C, and the shading rate between 20% and 80%.

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