Composition for effectively reducing after-sun injury and application of composition in sunscreen product

Abstract

The invention discloses a composition capable of effectively reducing after-sun injury and application of the composition in sunscreen products, and particularly belongs to the technical field of skin care product raw materials. The composition is composed of a lessonia nigrescens extract, a centella asiatica extract, a haematococcus pluvialis extract and a hedyotis diffusa extract, the haematococcus pluvialis extract is prepared through an alkali ultrasonic alcohol precipitation extraction technology, and active ingredients are effectively reserved. The composition has excellent antioxidant, anti-inflammatory and photoprotective properties, can be applied to preparation of skin care products with antioxidant, anti-inflammatory, moisturizing and soothing effects, is especially suitable for sunscreen products for reducing after-sun injury, and can reduce the use amount of physical / chemical sunscreen agents and reduce skin irritation.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care raw materials, and in particular relates to a composition capable of effectively reducing post-sun damage and application thereof in sunscreen products. BACKGROUND

[0002] With the increasing concern about skin damage caused by external light radiation such as ultraviolet (UV) and blue light, the demand for sunscreen and post-sun repair skin care products is growing. Existing sunscreen products mostly rely on physical sunscreen agents (such as titanium dioxide and zinc oxide) or chemical sunscreen agents (such as methoxyethylhexyl cinnamate) to achieve light protection, but physical sunscreen agents are prone to produce a heavy and whitening feeling, and chemical sunscreen agents may irritate sensitive skin. At the same time, most products only focus on "blocking light radiation", and have limited repair ability for skin oxidative damage, inflammatory response (such as redness and stinging), and decreased cell vitality caused after sun exposure.

[0003] In addition, the efficacy of single plant extract in existing skin care products is limited: for example, although single Chlorella vulgaris extract has strong antioxidant properties, it lacks anti-inflammatory and skin barrier repair ability; although single Centella asiatica extract can relieve inflammation, it lacks strong photoprotective effect. It is difficult to achieve comprehensive post-sun damage improvement effect of "antioxidation-anti-inflammation-photoprotection-repair" through single or a small number of ingredients, and it is difficult to meet the needs of consumers for mild and efficient post-sun protection and repair.

[0004] Therefore, it is a key requirement in the field of skin care to develop a composition with multiple ingredients synergistically acting to reduce post-sun damage from multiple dimensions. SUMMARY

[0005] In view of the deficiencies of the prior art, the purpose of the present application is to provide a composition capable of effectively reducing post-sun damage and improving the photoprotective effect of skin, and application thereof in sunscreen products.

[0006] To achieve the above-mentioned purpose, the following technical solutions are disclosed: In a first aspect, the present application provides a composition capable of effectively reducing post-sun damage, which contains the following components: Gigartina skottsbergii extract, Centella asiatica extract, Chlorella vulgaris extract and Sida acuta extract.

[0007] Preferably, the composition contains the following components by mass fraction: Gigartina skottsbergii extract 15-20 parts; Centella asiatica extract 2-4 parts; Chlorella vulgaris extract 1-2 parts; Sida acuta extract 6-8 parts.

[0008] Further preferably, the composition contains the following components by mass fraction: Dilute black giant seaweed extract 18 parts; Centella asiatica extract 3 parts; Haematococcus pluvialis extract 1.5 parts; Oldenlandia diffusa extract 7 parts.

[0009] Further preferably, the preparation method of the dilute black giant seaweed extract comprises the following steps: Step 1. Take dry Haematococcus pluvialis and add 1.5-2.5wt% NaOH aqueous solution, stir and mix uniformly to form a suspension; Step 2. Ultrasonically treat the suspension for 10-15min, heat the ultrasonically treated mixture in a water bath at 80-85℃ for 2-3h, centrifuge the extracted mixture to collect the supernatant, discard the precipitate, and obtain an extract containing polysaccharides; Step 3. Add anhydrous ethanol to the extract, and after the addition is complete, stand at 4℃ for 16-18h, centrifuge the alcohol-sedimented mixture to collect the precipitate; Step 4. Dry the precipitate and then freeze-dry it to obtain the Haematococcus pluvialis extract.

[0010] More preferably, in Step 1, accurately weigh a certain amount of dry Haematococcus pluvialis, place it in a container, add 1.5-2.5wt% NaOH aqueous solution according to a solid-liquid ratio of 1:25-30(g / mL), stir thoroughly to mix uniformly, and form a suspension.

[0011] More preferably, in Step 2, ultrasonically treat the suspension for 10-15min, wherein the ultrasonic frequency is 28kHz, heat the ultrasonically treated mixture in a water bath to 80-85℃, heat extract at a rotation speed of 150-200r / min for 2-3h, centrifuge the extracted mixture at 8000-10000r / min for 10-15min, collect the supernatant, discard the precipitate, and obtain an extract containing polysaccharides.

[0012] More preferably, in Step 3, add 4 times the volume of anhydrous ethanol to the extract, and after the addition is complete, stand at 4℃ for 16-18h, centrifuge the alcohol-sedimented mixture at a rotation speed of 8000-10000r / min for 10-15min, and after centrifugation, discard the supernatant and collect the precipitate.

[0013] More preferably, in Step 4, add 70-80v / v% ethanol aqueous solution to the precipitate, centrifuge at 8000-10000r / min for 10-15min, discard the supernatant, repeat the washing 1-2 times, dry the washed precipitate at 60℃ for 2-3h, and then vacuum freeze-dry it to obtain the Haematococcus pluvialis extract.

[0014] In a second aspect, the present application provides use of the composition of the first aspect in the preparation of a skin care product having antioxidant, anti-inflammatory, moisturizing and / or soothing efficacy.

[0015] In a third aspect, the present application provides use of the composition of the first aspect in the preparation of a sunscreen product capable of effectively reducing post-sun damage.

[0016] In the present application: The Lessonia nigr escens extract contains rich polysaccharides, minerals and active peptides, which can form a mild moisturizing film on the skin surface, increase the water content of the skin, enhance the skin barrier function, and reduce post-sun skin dryness and desquamation. On the other hand, it can help resist direct damage of ultraviolet and blue light to skin cells, and provide basic light protection support for the skin. It is the core component of the composition for "barrier repair + basic light protection".

[0017] The Centella asiatica extract contains active substances such as asiaticoside and hydroxyl asiaticoside, which can significantly inhibit the secretion of post-sun skin inflammatory factors (such as IL-6), relieve post-sun skin inflammation such as redness, stinging and itching, and promote skin fibroblast activity to accelerate the repair process of post-sun damaged skin. It is a key component of the composition for "anti-inflammatory + repair".

[0018] The Haematococcus pluvialis extract is rich in polysaccharide components and has strong antioxidant activity, which can efficiently scavenge DPPH free radicals generated in post-sun skin, reduce oxidative stress damage to skin cells, enhance the skin's ability to resist ultraviolet and blue light, and reduce the rate of cell apoptosis induced by light radiation. It is a core component of the composition for "strong antioxidant + core light protection". The "ultrasonic-assisted hot soaking + alcohol precipitation + freeze-drying" preparation process provided in the present application can maximize the activity of polysaccharides and ensure the efficacy.

[0019] The Hedyotis diffusa extract contains active ingredients such as flavonoids and alkaloids, which have auxiliary anti-inflammatory and soothing effects: they can synergize with the asiaticoside extract to further inhibit the release of inflammatory factors and enhance the overall anti-inflammatory effect; at the same time, they can relieve the discomfort of the skin caused by light radiation and reduce the sensitivity of the skin to irritation. It is an important component of the composition for "anti-inflammatory synergy + soothing assistance".

[0020] The present application has found that the above four components form a multi-dimensional synergistic system, which respectively targets the four core problems of "oxidation-inflammation-cell damage-barrier destruction" of post-sun damage, and achieves comprehensive efficacy in multiple dimensions and multiple aspects. A single component formula cannot achieve the same effect.

[0021] The present application has the following beneficial effects: 1. The composition provided by the present application can effectively remove post-sun skin free radicals, can effectively relieve post-sun inflammation, can significantly reduce the damage of light radiation to skin cells, and can improve post-sun skin problems from multiple dimensions. 2. The core component of the composition provided by the present application is a natural plant extract, and there is no irritation of chemical sunscreen agents and the heavy feeling of physical sunscreen agents, meanwhile, the excellent light protection performance can reduce the addition amount of physical / chemical sunscreen agents in sunscreen products, further reduce the risk of skin irritation, and is suitable for sensitive skin, dry skin, oily skin and various skin types, and can be widely applied to sunscreen cream, post-sun repair cream, moisturizing cream and various skin care products. DETAILED DESCRIPTION

[0022] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples. Those skilled in the art should understand that the specific examples described herein are only used to explain the present application, and are not used to limit the present application.

[0023] Unless otherwise specified, the test methods used in the examples and comparative examples are conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the examples and comparative examples, unless otherwise specified, are mass percentages.

[0024] In the present application: Lessonia nigrscens extract: Lessonia nigrscens extract, model number: ZnPro-II, purchased from Qingdao Haida Marine Oligosaccharide Technology Co., Ltd.; Centella asiatica extract: Centella asiatica extract, purchased from Shandong Furuida Biological Technology Co., Ltd.; Hedyotis diffusa extract: Hedyotis diffusa extract, purchased from Guangzhou Galen Biotechnology Co., Ltd.; Haematococcus pluvialis: purchased from Yunnan Aier Biotechnology Co., Ltd.

[0025] Preparation of Haematococcus pluvialis extract Step 1. Accurately weigh a certain amount of dried Haematococcus pluvialis, place it in a container, add 1.5-2.5wt% NaOH aqueous solution according to the solid-liquid ratio of 1:25-30 (g / mL), fully stir to make it uniformly mixed, and form a suspension; Step 2. The container containing the suspension is subjected to ultrasonic treatment in an ice water bath for 10-15 min, with an ultrasonic frequency of 28 kHz, and the mixture after ultrasonic treatment is placed in a constant temperature water bath at 80°C and heated for extraction at a rotation speed of 150 r / min for 2 h. The mixture after extraction is centrifuged at a rotation speed of 8000-10000 r / min for 10-15 min, and the supernatant is collected, and the precipitate is discarded, to obtain an extract containing polysaccharides; Step 3. 4 times the volume of anhydrous ethanol is slowly added to the extract, and the mixture is slowly stirred with a glass rod. After the addition is completed, the mixture is placed in a refrigerator at 4°C for 16-18 h to allow the polysaccharides to fully precipitate. The mixture after alcohol precipitation is centrifuged at a rotation speed of 8000-10000 r / min for 10-15 min, and the supernatant is discarded after centrifugation to collect the precipitate; Step 4. 70-80 v / v% ethanol aqueous solution is added to the precipitate to cover the precipitate, and the mixture is again centrifuged at a rotation speed of 8000-10000 r / min for 10-15 min. The supernatant is discarded, and the washing is repeated 1-2 times. The washed precipitate is transferred to a 60°C oven and dried for 2-3 h, and then vacuum freeze-dried to obtain a Haematococcus pluvialis extract.

[0026] Composition for reducing post-sun damage The raw materials are weighed according to the mass parts in Table 1, and the composition for reducing post-sun damage is obtained after mixing uniformly.

[0027] Table 1 Mass parts of raw materials

[0028] Performance test 1. Antioxidant test: The above-prepared compositions 1-7 are diluted with deionized water to obtain test sample liquids 1-7 with a concentration of 50 μg / mL, and a DPPH free radical scavenging ability test is performed. The DPPH free radical scavenging ability reflects the antioxidant ability of the substance to some extent.

[0029] The test method is as follows: The DPPH reagent is accurately weighed and prepared into a 0.04 mg / mL DPPH solution with 50 v / v% ethanol aqueous solution. Under light-proof conditions, the DPPH solution and different test substances are added to a 96-well plate, mixed thoroughly, and incubated at room temperature for 30 min. The absorbance is measured at 517 nm on an enzyme-labeled instrument. Each determination requires three groups of test sample liquid, blank and control, with three parallel samples in each group, as follows: Sample group: 100 μL of test sample liquid + 100 μL of DPPH solution Blank group: 100 μL of test sample liquid + 100 μL of 50 v / v% ethanol aqueous solution Control group: 100 μL 50 v / v% ethanol aqueous solution + 100 μL DPPH solution The DPPH scavenging rate was calculated according to the following formula: DPPH scavenging rate = [1- (sample group-blank group) / control group] x 100% The test results are shown in Table 2: Table 2 Antioxidant test results Group DPPH scavenging rate / % Test sample liquid 1 (corresponding to composition 1) 87.73% Test sample liquid 2 (corresponding to composition 2) 88.20% Test sample liquid 3 (corresponding to composition 3) 86.92% Test sample liquid 4 (corresponding to composition 4) 71.59% Test sample liquid 5 (corresponding to composition 5) 76.42% Test sample liquid 6 (corresponding to composition 6) 80.68% Test sample liquid 7 (corresponding to composition 7) 73.68% Result analysis: According to the results in Table 2, it can be seen that compositions 1-3 have excellent DPPH free radical scavenging ability, and compared with compositions 4-7, it is found that the lack of any component will reduce the free radical scavenging rate of the composition, which shows that the giant kelp extract, centella asiatica extract, oldenlandia diffusa extract and haematococcus pluvialis extract in the composition complement each other and achieve good antioxidant effect.

[0030] 2. Anti-inflammatory test: In this test, macrophage Ana-1 was used as the research object, and the effect of compositions 1-7 on the secretion of inflammatory factor IL-6 was detected by ELISA.

[0031] The test method is as follows: Macrophage Ana-1 was inoculated in a 96-well plate at 1 x 10 4 Macrophage Ana-1 was inoculated in a 96-well plate at 1 x 10 4 The LPS solution was diluted to 3 μg / mL, 50 μL / well was added to the well plate, and the well plate was placed in a 37℃, 5% CO2 incubator for 4h. Compositions 1-7 were diluted to a concentration of 100 μg / mL with complete culture medium as a diluent, 100 μL / well was added to the cells, and the control group was the diluent and 50 v / v% ethanol aqueous solution, each group was repeated 6 wells, and the well plate was placed in a 37℃, 5% CO2 incubator for 24h. Finally, the IL-6 content was detected by ELISA method, the standard curve regression equation was calculated according to the known concentration of the standard and the measured OD value, the OD value of the sample well was substituted into the calculation to measure the concentration of the sample, and then multiplied by the dilution factor to obtain the actual IL-6 concentration of the original sample.

[0032] The inhibition rate of the sample on IL-6 was calculated by the following formula: Inhibition rate (%) = (1- sample group IL-6 content / control group IL-6 content) x 100% The test results are shown in Table 3: Table 3 Anti-inflammatory test results Group Inflammatory factor IL-6 inhibition rate / % Composition 1 70.53 Composition 2 71.19 Composition 3 70.71 Composition 4 32.30*** Composition 5 28.12*** Composition 6 41.03*** Composition 7 39.15*** Note: “*” means significant difference compared with composition 2, P<0.05, “**” P<0.01, “***” P<0.001.

[0033] Result analysis: According to the test results in Table 3, compositions 1-7 all have certain inhibitory effect on IL-6, among which, the anti-inflammatory performance of compositions 4-7 is significantly decreased compared with composition 2, which shows that the formula system composed of dark black giant seaweed extract, centella asiatica extract, oldenlandia diffusa extract and haematococcus extract has excellent performance in anti-inflammatory soothing, and the components in the system achieve indispensable synergistic promotion, which can effectively inhibit the expression of inflammatory factors.

[0034] 3. Light protection test:

[0035] This test carries out cell survival rate test on compositions 1-7. The survival rate of human immortal keratinocytes (HaCat) after ultraviolet and blue light irradiation can reflect the protection of substances against ultraviolet and blue light to a certain extent.

[0036] The test method is as follows: First, the cell safety test concentration of the composition is determined by MTT cytotoxicity test.

[0037] According to 2×10 4 The immortal keratinocyte suspension is inoculated in a 96-well plate at a density of 100 μL / well, and placed in a 37°C, 5% CO2 incubator for 24 h. Compositions 1-7 are diluted to a concentration of 100 μg / mL with complete culture medium as diluent, and then added to the cells, 100 μL / well. The positive control group and the negative control group are added with the same volume of 50 v / v% ethanol aqueous solution instead of drug addition, and placed in a 37°C, 5% CO2 incubator for 24 h. The cells in the composition 1-7 group and the positive control group are irradiated with UV for 20 min and blue light for 3 h respectively, and the negative control group is not treated with light. Then they are placed in the incubator for 24 h, and finally the MTT experiment is used to detect the change of cell viability.

[0038] The calculation formula is as follows: Cell viability percentage (%) = sample well average OD value / negative control group average OD value x 100% The test results are shown in Table 4: Table 4. Light protection test results

[0039] Note: “*” means significant difference compared with composition 2, P<0.05, “**” P<0.01, “***” P<0.001.

[0040] Results analysis: According to the results of Table 4, compared with the positive control group, the activity of HaCat cells treated with compositions 1-3 was significantly increased after ultraviolet and blue light irradiation, and was significantly higher than compositions 4-7, which indicated that the composition composed of extract of Gigartina tenella, extract of Centella asiatica, extract of Oldenlandia diffusa and extract of Haematococcus pluvialis could effectively resist the damage of ultraviolet and blue light, and play a photoprotective role. The composition provided by the application can be applied to skin care products with sunscreen effect, and the use of physical sunscreen agents and chemical sunscreen agents can be reduced.

[0041] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the foregoing embodiments of the present application are described in detail, for those skilled in the art, it still can be modified, or part of the technical features of the equivalent replacement. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A composition effective in reducing post-sun damage, characterized in that, The composition contains the following components: Ascophyllum nodosum extract, Centella asiatica extract, Haematococcus pluvialis extract and Sorsastrum alturnus extract.

2. The composition of claim 1, wherein, The composition contains the following components by mass fraction: Ascophyllum nodosum extract 15-20 parts; Centella asiatica extract 2-4 parts; Haematococcus pluvialis extract 1-2 parts; Sorsastrum alturnus extract 6-8 parts.

3. The composition of claim 2, wherein, The preparation method of the Ascophyllum nodosum extract comprises the following steps: Step 1. Take dry Haematococcus pluvialis and add 1.5-2.5wt% NaOH aqueous solution to stir and mix uniformly to form a suspension; Step 2. Ultrasonically treat the suspension for 10-15min, heat the mixture after ultrasonic treatment at 80-85℃ for 2-3h, centrifuge the mixture after extraction to collect the supernatant and discard the precipitate to obtain an extract containing polysaccharides; Step 3. Add anhydrous ethanol to the extract, and after the addition is completed, stand at 4℃ for 16-18h, centrifuge the mixture after alcohol precipitation to collect the precipitate; Step 4. Dry the precipitate and then freeze-dry to obtain the Haematococcus pluvialis extract.

4. The composition of claim 3, wherein, In step 1, accurately weigh a certain amount of dry Haematococcus pluvialis, place it in a container, and add 1.5-2.5wt% NaOH aqueous solution according to a solid-liquid ratio of 1:25-30(g / mL), stir thoroughly to mix uniformly, and form a suspension.

5. The composition of claim 3, wherein In step 2, ultrasonically treat the suspension for 10-15min, and the ultrasonic frequency is 28kHz. Heat the mixture after ultrasonic treatment in a water bath to 80-85℃, heat extract at a rotation speed of 150-200r / min for 2-3h, centrifuge the mixture after extraction at 8000-10000r / min for 10-15min, collect the supernatant and discard the precipitate to obtain an extract containing polysaccharides.

6. The composition of claim 3, wherein In step 3, add 4 times the volume of anhydrous ethanol to the extract, and after the addition is completed, stand at 4℃ for 16-18h, centrifuge the mixture after alcohol precipitation at a rotation speed of 8000-10000r / min for 10-15min, and after centrifugation, pour off the supernatant and collect the precipitate.

7. The composition of claim 3, wherein In step 4, add 70-80v / v% ethanol aqueous solution to the precipitate, centrifuge at 8000-10000r / min for 10-15min, pour off the supernatant, repeat the washing 1-2 times, dry the washed precipitate at 60℃ for 2-3h, and then vacuum freeze-dry to obtain the Haematococcus pluvialis extract.

8. Use of the composition of any one of claims 1-7 in the preparation of a skin care product having antioxidant, anti-inflammatory, moisturizing and / or soothing effects.

9. Use of the composition of any one of claims 1-7 in the preparation of a sunscreen product that can effectively reduce post-sun damage.

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