Centrifugal cup for cell treatment

Abstract

The centrifugal cup for cell treatment comprises a centrifugal cup body, a centrifugal cup head and a first flow guide pipe, the top of the centrifugal cup body is rotatably connected with the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup, and a centrifugal treatment bin is arranged in the centrifugal cup body; an inlet, a first outlet and a second outlet are formed in the top of the centrifugal cup head, the bottom of the centrifugal cup head penetrates through the top of the centrifugal cup body and extends into the centrifugal cup body, and the top of the centrifugal cup body is rotatably connected with the middle of the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup; the first flow guide pipe is arranged in the centrifugal cup body, the first end of the first flow guide pipe is fixedly connected with the inner side of the top of the centrifugal cup body, and the second end of the first flow guide pipe is arranged at the bottom of the centrifugal treatment bin and communicates with the outer edge of the centrifugal treatment bin; the inlet is communicated with the centrifugal treatment bin; the first outlet is communicated with the inner side of the centrifugal treatment bin through an interlayer flow channel in the side wall of the centrifugal cup body, and the interlayer flow channel is located above the outer edge; the second outlet is communicated with the outer edge through a first flow guide pipe.

Description

[0001] Cross-references to related applications

[0002] This application claims priority to Chinese patent application No. 202410995784X, filed on July 23, 2024, the entire contents of which are incorporated herein by reference. Technical Field

[0003] This invention belongs to the field of cell processing technology, and specifically relates to a centrifuge cup for cell processing. Background Technology

[0004] Cell therapy is an innovative approach that uses bioengineering methods to obtain cells with specific functions, and then expands and specially cultures them in vitro to enhance immunity, kill pathogens and tumor cells, thereby achieving the goal of treating diseases. After in vitro expansion and special culture, the specially cultured cells need to be isolated and harvested, including the isolation, concentration, washing, and replacement of the culture medium for the target cells. Cell concentration typically involves converting large culture systems into smaller formulation systems, that is, concentrating a culture system from several hundred milliliters to 20 liters to a formulation system of 100 to 500 milliliters, with a concentration volume usually more than 10 times.

[0005] For large-volume cell concentration, manual processing is typically cumbersome, yields inconsistent results, varies significantly in recovery efficiency, and may even lead to contamination. In addition to manual operation, some closed, automated separation devices are now available that can improve product consistency and effectively ensure product sterility.

[0006] Patent publication number WO2000038762 discloses a piston-type centrifuge cup with two inlets and / or outlets, capable of quantitatively drawing in or expelling cell fluid. Based on the density gradient centrifugation principle, under centrifugal force, denser cells are centrifuged to the side wall of the centrifuge cup, while less dense liquids remain in the center. As the piston pushes upward, the liquid in the center is discharged from the central outlet. Real-time detection and control of the piston position allows control of the cell fluid volume within the centrifuge cup, obtaining the set target cell solution. However, because this device has a limited single-cycle processing volume, generally not exceeding 220 ml of cell fluid, while the required cell fluid volume is usually greater than 220 ml, multiple cycles are necessary. Furthermore, the centrifuge cup needs to be rinsed between cycles, which is time-consuming and negatively impacts cell viability. Moreover, as the amount of concentrate in the centrifuge cup increases, the centrifugal force on cells closer to the center decreases, increasing the risk of discharge with waste liquid and reducing cell recovery rate.

[0007] The patent with publication number US20200141942A1 discloses a cylindrical centrifugal cup which comprises an inlet and an outlet. Due to the structural limitation of the centrifugal cup, the maximum single concentration volume is about 40 milliliters. Therefore, for large sample volumes, multiple cycles of processing are required. If the concentration volume is greater than 40 milliliters, the outlet is blocked, causing the waste liquid in the middle part to be unable to be discharged. Multiple cycles of processing cause the concentrated cells at the edge to be squeezed against the side wall of the centrifugal cup for a long time, affecting the activity of the cells.

[0008] The centrifugal device of the prior art is difficult to be applied to large-volume cell concentration processing, and the collected cell concentration and cell purity are low. Processing large-volume samples has the problems of long processing time, low recovery rate of harvested cells, and low activity. Large-volume cell concentration processing often requires multiple cycles of processing, and the process of the supporting instrument is relatively complex. A large amount of buffer solution is also required to rinse the centrifugal cup, which increases the problem of additional loss. SUMMARY

[0009] The present application aims to solve the problems of the prior art and provides a centrifugal cup, particularly a centrifugal cup for cell processing.

[0010] To solve the above technical problems, the present application adopts the following technical scheme: a centrifugal cup for cell processing, comprising a centrifugal cup body, a centrifugal cup head, and a first flow guide pipe, wherein the top of the centrifugal cup body is rotatably and sealingly connected with the centrifugal cup head along the longitudinal axis of the centrifugal cup, and the inside of the centrifugal cup body is provided with a centrifugal processing chamber; the top of the centrifugal cup head is provided with an inlet, a first outlet, and a second outlet, the bottom of the centrifugal cup head penetrates through the top of the centrifugal cup body and extends into the inside of the centrifugal cup body, and the top of the centrifugal cup body is rotatably and sealingly connected with the middle part of the centrifugal cup head along the longitudinal axis of the centrifugal cup; the first flow guide pipe is arranged in the centrifugal cup body, the first end of the first flow guide pipe is fixedly connected with the inside of the top of the centrifugal cup body, and the second end of the first flow guide pipe is arranged at the bottom of the centrifugal processing chamber and communicates with the outer edge of the centrifugal processing chamber; the inlet communicates with the centrifugal processing chamber; the first outlet communicates with the inside of the centrifugal processing chamber through a interlayer flow channel in the side wall of the centrifugal cup body; the interlayer flow channel is located above the outer edge of the centrifugal processing chamber; and the second outlet communicates with the outer edge of the centrifugal processing chamber through the first flow guide pipe.

[0011] Further, the centrifugal cup comprises a first sealing part, a second sealing part and a third sealing part, which are arranged in the accommodating cavity in the top of the centrifugal cup body in an axial direction, the first sealing part is close to the bottom of the centrifugal cup head, the third sealing part is away from the bottom of the centrifugal cup head, and the second sealing part is between the first sealing part and the third sealing part, the bottom outer surface of the centrifugal cup head and the inner surface of the top of the centrifugal cup body are connected through the first sealing part, the second sealing part and the third sealing part; the bottom of the first sealing part and the inner side through hole of the top of the centrifugal cup body form a first sealing space, and the second outlet communicates with the first flow guide pipe through the first sealing space; the top of the first sealing part and the bottom of the second sealing part form a second sealing space, and the inlet communicates with the centrifugal processing chamber through the second sealing space; the top of the second sealing part and the bottom of the third sealing part form a third sealing space, and the first outlet communicates with the interlayer flow channel through the third sealing space.

[0012] Further, the accommodating cavity comprises a first layer annular step, a second layer annular step and a third layer annular step, the first layer annular step is close to the bottom of the centrifugal cup head, the third layer annular step is away from the bottom of the centrifugal cup head, and the second layer annular step is between the first layer annular step and the second layer annular step, the first sealing part is placed in the first layer annular step, the second sealing part is placed in the second layer annular step, and the third sealing part is placed in the third layer annular step.

[0013] Further, the inner diameters of the first layer annular step, the second layer annular step and the third layer annular step gradually increase.

[0014] Further, the first sealing part comprises a first sealing ring and a first pressing ring, the first sealing ring is arranged in the first layer annular step and sleeved on the outer surface of the bottom of the centrifugal cup head, the outer surface of the first sealing ring forms a static seal with the inner surface of the first layer annular step, and the inner surface of the first sealing ring forms a dynamic seal with the outer surface of the bottom of the centrifugal cup head; the bottom of the first sealing ring and the inner side through hole form the first sealing space; the first pressing ring is arranged on the top of the first sealing ring and sleeved on the outer surface of the bottom of the centrifugal cup head, the top of the first pressing ring and the bottom of the second sealing part form the second sealing space, the first pressing ring is pressed with the inner side of the top of the centrifugal cup body to form a liquid inlet channel opening, and the inlet communicates with the centrifugal processing chamber through the second sealing space and the liquid inlet channel opening.

[0015] Further, the second sealing part comprises a second compression ring, a second sealing ring and a third compression ring; the second compression ring is arranged in the second annular step and fixedly connected with the second annular step; the second sealing ring is arranged inside the second compression ring and sleeved outside the bottom of the centrifugal cup head, the outer surface of the second sealing ring forms a static seal with the inner surface of the second compression ring, and the inner surface of the second sealing ring forms a dynamic seal with the outer surface of the bottom of the centrifugal cup head; the second sealing space and the liquid inlet channel are both arranged between the top of the first compression ring and the bottom of the second sealing part; the third compression ring is arranged at the top of the second sealing ring and the top of the second compression ring and sleeved on the outer surface of the bottom of the centrifugal cup head, the top of the third compression ring and the bottom of the third sealing part form the third sealing space, and the third compression ring is press-fitted with the inner side of the top of the centrifugal cup body to form an intermediate channel for discharging supernatant, and the first outlet is communicated with the interlayer flow channel through the third sealing space and the intermediate channel for discharging supernatant.

[0016] Further, the third sealing part comprises a fourth compression ring and a third sealing ring; the fourth compression ring is arranged in the third annular step and fixedly connected with the third annular step; the third sealing ring is arranged inside the fourth compression ring and sleeved outside the bottom of the centrifugal cup head, the outer surface of the third sealing ring forms a static seal with the inner surface of the fourth compression ring, and the inner surface of the third sealing ring forms a dynamic seal with the outer surface of the bottom of the centrifugal cup head; the third sealing space and the intermediate channel for discharging supernatant are both arranged between the top of the third compression ring and the bottom of the third sealing part.

[0017] Further, the middle part of the centrifugal cup head is provided with an annular boss, the inner side of the top of the centrifugal cup body is connected with the circumferential sidewall of the annular boss through a connecting piece, the bottom surface of the connecting piece is press-fitted on the top surface of the fourth compression ring, and the bottom surface of the annular boss is in contact with the top surface of the third sealing ring.

[0018] Further, the centrifugal cup comprises a second flow guide pipe, the second flow guide pipe is arranged in the centrifugal cup body and sleeved outside the first flow guide pipe, the first end of the second flow guide pipe is fixedly connected with the outer surface of the containing cavity, and the second end of the second flow guide pipe is arranged close to the bottom of the centrifugal processing bin, and the inlet is communicated with the centrifugal processing bin through the second sealing space, the liquid inlet channel and the second flow guide pipe.

[0019] Further, the second flow guide pipe is a tapered pipe with a small top aperture and a large bottom aperture, and the centrifugal processing bin is a tapered bin with a small top aperture and a large bottom aperture.

[0020] Further, the first end of the first flow guide pipe is fixedly connected with the inner hole of the top of the centrifugal cup, the bottom of the first flow guide pipe is fixedly connected with the bottom wall of the centrifugal cup, and the second end of the first flow guide pipe is arranged at the circumferential edge of the bottom wall of the centrifugal cup and close to the outer edge of the centrifugal processing bin.

[0021] Further, the first flow guide pipe is an L-shaped cell flow channel.

[0022] Further, a first flow guide part is arranged on the bottom wall of the centrifugal cup and close to the second end port of the first flow guide pipe.

[0023] Further, the bottom of the interlayer flow channel is provided with a supernatant discharge channel port in communication with the inner side of the centrifugal processing bin, the supernatant discharge channel port is arranged opposite to the second end port of the first flow guide pipe, and the top of the interlayer flow channel is in communication with the supernatant discharge intermediate channel port.

[0024] Further, a second flow guide part is arranged on the side wall of the centrifugal cup and close to the supernatant discharge channel port.

[0025] Further, the side wall of the centrifugal cup comprises a first side wall and a second side wall arranged in the first side wall, and the interlayer flow channel is arranged between the outer surface of the second side wall and the inner surface of the first side wall.

[0026] Further, the centrifugal cup comprises a main body shell and a base, the top of the main body shell is rotatably connected with the centrifugal cup head along the longitudinal axis of the centrifugal cup, the circumferential edge of the bottom of the main body shell is fixedly and sealingly connected with the circumferential edge of the base, and the inner wall of the main body shell and the inner surface of the base surround the centrifugal processing bin.

[0027] Further, the inlet is in communication with the second sealing space and the liquid inlet channel port through a liquid inlet flow guide channel arranged in the centrifugal cup head.

[0028] Further, the supernatant discharge intermediate channel port is in communication with the first outlet through the third sealing space and a supernatant flow guide channel arranged in the centrifugal cup head.

[0029] Further, the first end of the first flow guide pipe is in communication with the second outlet through the first sealing space and a cell liquid flow guide channel arranged in the centrifugal cup head.

[0030] Compared with the prior art, the beneficial effects of the present application are:

[0031] 1. The centrifugal cup for cell processing of the present application comprises a centrifugal cup body and a centrifugal cup head, a centrifugal processing chamber is arranged in the centrifugal cup body, a first outlet and a second outlet are arranged on the centrifugal cup head, the supernatant inside the centrifugal processing chamber can be discharged in real time from the first outlet to a waste liquid bag, and the concentrated cell liquid centrifuged to the outer edge of the centrifugal processing chamber can be discharged in real time from the second outlet, a sandwich flow channel is arranged above the outer edge of the centrifugal processing chamber, the supernatant can be discharged first, and the concentrated cell liquid can be discharged last, so that higher concentration cells and higher purity cells can be collected, and the concentrated cell liquid is not squeezed by centrifugation for a long time, and the high activity of the cells is maintained.

[0032] 2. The centrifugal cup for cell processing of the present application can not only simply and efficiently realize continuous flow processing of cell liquid, but also facilitate continuous recovery of cells, has high recovery rate, fast processing speed, and does not need to rinse the centrifugal cup again, thereby saving time and buffer solution.

[0033] 3. The centrifugal cup for cell processing of the present application has simple structure, is convenient for production, manufacturing and installation, is conducive to reducing production cost, is economical, and is convenient to use.

[0034] 4. The centrifugal cup for cell processing of the present application is provided with a first sealing part, a second sealing part, a third sealing part, a first sealing space, a second sealing space and a third sealing space, which can not only improve the sealing effect, but also make the flow channel in the rotating centrifugal cup body not directly contact with the flow channel in the stationary centrifugal cup head, so that the concentricity requirement of the centrifugal cup is greatly reduced, and the centrifugal cup can be suitable for rotary sealing, has small friction resistance, large sealing pressure, good sealing effect, and does not produce insoluble particles, thereby preventing pollution of the cell liquid and / or the concentrated cells.

[0035] 5. The centrifugal cup for cell processing of the present application is provided with a second flow guide pipe, so that the centrifugal processing chamber is formed between the outer surface of the side wall of the second flow guide pipe and the inner surface of the side wall and the inner surface of the bottom wall of the centrifugal cup body, the cell liquid is facilitated to flow to the centrifugal processing chamber, and the separation effect is better under the larger centrifugal force.

[0036] 6. The inner second flow guide pipe of the centrifugal cup for cell processing of the present application is arranged in a conical pipe, and the centrifugal processing chamber is arranged in a conical ring, so that the cells are subjected to downward force when the cell liquid is centrifuged, the cells are facilitated to separate from the supernatant, the recovery rate is improved, and the cells are prevented from being discharged with the supernatant.

[0037] 7. The first flow guide pipe of the centrifugal cup for cell processing of the present application is an L-shaped cell discharge flow channel, which can facilitate the concentrated cell liquid to be discharged from the outer edge of the centrifugal processing chamber to the second outlet, has simple structure, is convenient for production, manufacturing and installation, is conducive to reducing production cost, and is convenient to use.

[0038] 8、The centrifugal cup for cell processing of the present application, the outlet channel port is opposite to the second end port of the first flow guide pipe, which can maintain the dynamic balance of the centrifugal cup and prevent the centrifugal cup from swinging. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 A top view schematic diagram of one embodiment of the centrifugal cup for cell processing of the present application is shown;

[0040] Figure 2 A radial cross-sectional schematic diagram of one embodiment of the centrifugal cup head of the centrifugal cup for cell processing of the present application is shown;

[0041] Figure 3 A cross-sectional schematic diagram of the A-A direction of Figure 1 is shown;

[0042] Figure 4 A cross-sectional schematic diagram of the B-B direction of Figure 1 is shown;

[0043] Figure 5 A local enlarged structural schematic diagram of one embodiment of the first sealing part, the second sealing part and the third sealing part of the centrifugal cup for cell processing of the present application is shown;

[0044] Figure 6 A liquid inlet flow direction schematic diagram of one embodiment of the centrifugal cup for cell processing of the present application is shown;

[0045] Figure 7 A liquid outlet flow direction schematic diagram of one embodiment of the centrifugal cup for cell processing of the present application is shown.

[0046] Wherein, 1-Centrifuge cup body; 11-Centrifuge processing chamber; 111-Outer edge; 112-Inner side; 12-Jackcase flow channel; 121-Supernatant discharge channel opening; 13-Accommodation cavity; 131-First annular step; 132-Second annular step; 133-Third annular step; 14-Inner through hole; 15-First sealing space; 16-Second sealing space; 17-Third sealing space; 18-Liquid inlet channel opening; 19-Supernatant discharge intermediate channel opening; 101-First sidewall; 102-Second sidewall; 103-Main shell; 104-Base; 2-Centrifuge Cup head; 21-Inlet; 22-First outlet; 23-Second outlet; 24-Annular boss; 25-Inlet guide channel; 26-Supernatant guide channel connection; 27-Cell fluid guide channel; 3-First guide tube; 31-Second end port; 4-First sealing part; 41-First sealing ring; 42-First pressure ring; 5-Second sealing part; 51-Second pressure ring; 52-Second sealing ring; 53-Third pressure ring; 6-Third sealing part; 61-Fourth pressure ring; 62-Third sealing ring; 7-Connector; 8-Second guide tube; 9-First guide part; 10-Second guide part. Detailed Implementation

[0047] The present invention will be further described below with reference to the embodiments shown in the accompanying drawings.

[0048] The directional terms used in this invention, such as "inner," "outer," "axial," and "circumferential," are merely for illustrative purposes and are not intended to limit the invention.

[0049] like Figures 1 to 7 As shown, the centrifuge cup for cell processing of the present invention includes: a centrifuge cup body 1, a centrifuge cup head 2, and a first guide tube 3. Wherein,

[0050] The top of the centrifuge cup body 1 is rotatably and sealed to the centrifuge cup head 2 along the longitudinal axis of the centrifuge cup. The centrifuge cup body 1 is provided with a centrifugation processing chamber 11.

[0051] The centrifuge head 2 has an inlet 21, a first outlet 22, and a second outlet 23 at its top. The bottom of the centrifuge head 2 extends through the top of the centrifuge body 1 into the interior of the centrifuge body 1. The top of the centrifuge body 1 is rotatably and sealingly connected to the middle of the centrifuge head 2 along the longitudinal axis of the centrifuge body.

[0052] The first guide tube 3 is installed inside the centrifuge cup 1. The first end of the first guide tube 3 is fixedly connected to the inner side of the top of the centrifuge cup 1. The second end of the first guide tube 3 is installed at the bottom of the centrifuge processing chamber 11 and communicates with the outer edge 111 of the centrifuge processing chamber 11.

[0053] The inlet 21 is in communication with the centrifugal processing chamber 11 for the sample cell liquid to enter the centrifugal processing chamber 11 from the inlet 21.

[0054] The first outlet 22 is in communication with the inner side 112 of the centrifugal processing chamber 11 through the interlayer flow channel 12 in the side wall of the centrifugal cup 1 for the supernatant to flow out of the inner side 112 of the centrifugal processing chamber 11 through the interlayer flow channel 12 and the first outlet 22. The interlayer flow channel 12 is located above the outer edge 111 of the centrifugal processing chamber 11.

[0055] The second outlet 23 is in communication with the outer edge 111 of the centrifugal processing chamber 11 through the first flow guide pipe 3 for the concentrated cell liquid to flow out of the outer edge 111 of the centrifugal processing chamber 11 through the first flow guide pipe 3 and the second outlet 23.

[0056] Wherein, the sample cell liquid enters the bottom of the centrifugal processing chamber 11 from the inlet 21 and fills in the centrifugal processing chamber 11. The air in the centrifugal cup is squeezed out through the interlayer flow channel 12 and the first outlet 22. After the centrifugal cup 1 rotates at high speed for a certain time, the cells in the sample cell liquid and the supernatant (supernatant waste liquid) are gradually stratified under the action of centrifugal force, the concentrated cell liquid is accumulated at the outer edge 111 of the centrifugal processing chamber 11 (at the outer diameter edge of the centrifugal processing chamber 11), and the supernatant is located at the inner side 112 of the centrifugal processing chamber 11 (the part of the centrifugal processing chamber 11 except the outer edge 111). As the inlet 21 continuously enters the sample cell liquid, the supernatant in the inner side 112 of the centrifugal processing chamber 11 is squeezed or pumped through the interlayer flow channel 12 and the first outlet 22 and discharged to the waste liquid bag. The concentrated cell liquid at the outer edge 111 of the centrifugal processing chamber 11 is discharged through the first flow guide pipe 3 and the second outlet 23. And since the interlayer flow channel 12 is located above the outer edge 111 of the centrifugal processing chamber 11, the centrifugal cup can realize the discharge of the supernatant first and then the discharge of the concentrated cell liquid, so as to facilitate the realization of higher collection cell concentration and cell purity, and the concentrated cell liquid will not be squeezed by centrifugation for a long time, and the cell activity is high. The centrifugal cup can not only simply and efficiently realize continuous flow processing of cell liquid, but also facilitate continuous recovery of cells, has high recovery rate, fast processing speed, and does not need to rinse the centrifugal cup again, which saves time and buffer solution. At the same time, the centrifugal cup has simple structure, is convenient for production and installation, is conducive to reducing production cost, and is convenient to use.

[0057] In one specific embodiment, as Figures 3 to 7As shown, the centrifugal cup comprises: a first sealing part 4, a second sealing part 5 and a third sealing part 6. The first sealing part 4, the second sealing part 5 and the third sealing part 6 are all arranged axially in a containing cavity 13 in the top of the centrifugal cup body 1. The first sealing part 4 is close to the bottom of the centrifugal cup head 2, the third sealing part 6 is away from the bottom of the centrifugal cup head 2, and the second sealing part 5 is between the first sealing part 4 and the third sealing part 6. The outer surface of the bottom of the centrifugal cup head 2 and the inner surface of the top of the centrifugal cup body 1 are connected by the first sealing part 4, the second sealing part 5 and the third sealing part 6.

[0058] A first sealing space 15 is formed between the bottom of the first sealing part 4 and the inner through hole 14 of the top of the centrifugal cup body 1, and the second outlet 23 communicates with the first flow guide pipe 3 through the first sealing space 15.

[0059] A second sealing space 16 is formed between the top of the first sealing part 4 and the bottom of the second sealing part 5. The inlet 21 communicates with the centrifugal processing chamber 11 through the second sealing space 16.

[0060] A third sealing space 17 is formed between the top of the second sealing part 5 and the bottom of the third sealing part 6. The first outlet 22 communicates with the interlayer flow channel 12 through the third sealing space 17.

[0061] Among them, by using the first sealing part 4, the second sealing part 5 and the third sealing part 6, the top of the centrifugal cup body 1 can be rotatably and sealingly connected with the bottom of the centrifugal cup head 2, and by using the first sealing space 15, the second outlet 23 can be communicated with the first flow guide pipe 3 through the first sealing space 15, by using the second sealing space 16, the inlet 21 can be communicated with the centrifugal processing chamber 11 through the second sealing space 16, and by using the third sealing space 17, the first outlet 22 can be communicated with the interlayer flow channel 12 through the third sealing space 17. And by using the first sealing part 4, the second sealing part 5, the third sealing part 6, the first sealing space 15, the second sealing space 16 and the third sealing space 17, not only the sealing effect can be improved, but also the flow channel in the rotating centrifugal cup body 1 and the flow channel in the stationary centrifugal cup head 2 are not in direct contact, thereby greatly reducing the requirement for the concentricity of the centrifugal cup.

[0062] In a specific embodiment, as Figures 3 to 7As shown, the accommodating cavity 13 comprises: a first layer annular step 131, a second layer annular step 132, and a third layer annular step 133. The first layer annular step 131 is close to the bottom of the centrifugal cup head 2, the third layer annular step 133 is away from the bottom of the centrifugal cup head 2, and the second layer annular step 132 is between the first layer annular step 131 and the second layer annular step 132. The first sealing part 4 is placed in the first layer annular step 131, the second sealing part 5 is placed in the second layer annular step 132, and the third sealing part 6 is placed in the third layer annular step 133. Among them, by using the first layer annular step 131, the second layer annular step 132 and the third layer annular step 133, the stability and reliability of the placement of the first sealing part 4, the second sealing part 5 and the third sealing part 6 can be improved, and the sealing effect can be improved.

[0063] In a specific embodiment, as shown in Figures 3 to 7 The inner diameters of the first layer annular step 131, the second layer annular step 132 and the third layer annular step 133 gradually increase, which can facilitate the installation of the first sealing part 4, the second sealing part 5 and the third sealing part 6.

[0064] In a specific embodiment, as shown in Figures 3 to 7 In particular, as shown in Figure 5 The first sealing part 4 comprises: a first sealing ring 41 and a first compression ring 42. Among them,

[0065] The first sealing ring 41 is arranged in the first layer annular step 131 and is sleeved on the outside of the bottom of the centrifugal cup head 2. The outer surface of the first sealing ring 41 forms a static seal with the inner surface of the first layer annular step 131, and the inner surface of the first sealing ring 41 forms a dynamic seal with the outer surface of the bottom of the centrifugal cup head 2. The bottom of the first sealing ring 41 and the inner side through hole 14 form a first sealing space 15.

[0066] The first compression ring 42 is arranged at the top of the first sealing ring 41 and is sleeved on the outer surface of the bottom of the centrifugal cup head 2. The top of the first compression ring 42 and the bottom of the second sealing part 5 form a second sealing space 16, and the first compression ring 42 is pressed on the inner side of the top of the centrifugal cup body 2 to form a liquid inlet channel opening 18. The inlet 21 communicates with the centrifugal treatment bin 11 through the second sealing space 16 and the liquid inlet channel opening 18.

[0067] Among them, by using the first compression ring 42, the axial movement of the first sealing ring 41 can be avoided. At the same time, by using the first sealing ring 41 and the first compression ring 42, the sealing effect of the first sealing part 4 can be improved.

[0068] In a specific embodiment, as shown in Figures 3 to 7 The second sealing part 5 comprises: a second compression ring 51, a second sealing ring 52 and a third compression ring 53. Among them,

[0069] The second compression ring 51 is arranged in the second annular step 132 and fixedly connected with the second annular step 132.

[0070] The second sealing ring 52 is arranged inside the second compression ring 51 and sleeved outside the bottom of the centrifugal cup head 2. The outer surface of the second sealing ring 52 forms a static seal with the inner surface of the second compression ring 51, and the inner surface of the second sealing ring 52 forms a dynamic seal with the outer surface of the bottom of the centrifugal cup head 2. The second sealing space 16 and the liquid inlet passage 18 are both arranged between the top of the first compression ring 42 and the bottom of the second sealing part 5.

[0071] The third compression ring 53 is arranged on the top of the second sealing ring 52 and the top of the second compression ring 51, and sleeved on the outer surface of the bottom of the centrifugal cup head 2. The top of the third compression ring 53 forms a third sealing space 17 with the bottom of the third sealing part 6, and the third compression ring 53 is pressed on the inner side of the top of the centrifugal cup body 2 to form an intermediate passage 19 for discharging supernatant. The first outlet 22 communicates with the interlayer flow channel 12 through the third sealing space 17 and the intermediate passage 19 for discharging supernatant.

[0072] The third compression ring 53 can prevent the top of the second sealing ring 52 and the second compression ring 51 from moving axially. The second compression ring 51, the second sealing ring 52, and the third compression ring 53 can improve the sealing effect of the second sealing part 5.

[0073] In a specific embodiment, as shown in Figures 3 to 7 The third sealing part 6 includes a fourth compression ring 61 and a third sealing ring 62.

[0074] The fourth compression ring 61 is arranged in the third annular step 133 and fixedly connected with the third annular step 133.

[0075] The third sealing ring 62 is arranged inside the fourth compression ring 61 and sleeved outside the bottom of the centrifugal cup head 2. The outer surface of the third sealing ring 62 forms a static seal with the inner surface of the fourth compression ring 61, and the inner surface of the third sealing ring 62 forms a dynamic seal with the outer surface of the bottom of the centrifugal cup head 2. The third sealing space 17 and the intermediate passage 19 for discharging supernatant are both arranged between the top of the third compression ring 53 and the bottom of the third sealing part 6.

[0076] The fourth compression ring 61 and the third sealing ring 62 can improve the sealing effect of the third sealing part 6.

[0077] In a specific embodiment, as shown in Figures 3 to 7As shown, the middle part of the centrifugal cup head 2 is provided with an annular boss 24, the inner side of the top of the centrifugal cup body 1 is connected with the peripheral side wall of the annular boss 24 through the connecting piece 7, the bottom surface of the connecting piece 7 is pressed on the top surface of the fourth pressing ring 61, and the bottom surface of the annular boss 24 is in contact with the top surface of the third sealing ring 62. Among them, the connecting piece 7 can improve the sealing connection effect of the inner side of the top of the centrifugal cup body 1 and the annular boss 24 of the centrifugal cup head 2, and can avoid the axial movement of the fourth pressing ring 61, and the annular boss 24 can avoid the axial movement of the third sealing ring 62.

[0078] In one specific embodiment, as shown in Figures 3 to 7 The connecting piece 7 includes a bearing, which is simple in structure and convenient to use.

[0079] In one specific embodiment, as shown in Figures 3 to 7 The centrifugal cup includes a second flow guide pipe 8. The second flow guide pipe 8 is arranged in the centrifugal cup body 1 and sleeved outside the first flow guide pipe 3. The first end of the second flow guide pipe 8 is fixedly connected with the outer surface of the containing cavity 13, and the second end of the second flow guide pipe 8 is arranged at a position close to the bottom of the centrifugal processing chamber 11. The inlet 21 is communicated with the centrifugal processing chamber 11 through the second sealing space 16, the liquid inlet passage 18 and the second flow guide pipe 8. Among them, the second flow guide pipe 8 can make the centrifugal processing chamber 11 formed between the outer surface of the side wall of the second flow guide pipe 8 and the inner surface of the side wall and the inner surface of the bottom wall of the centrifugal cup body 1, so that the sample cell liquid can flow to the centrifugal processing chamber 11 more easily, and the centrifugal force is greater, and the separation effect is better.

[0080] In one specific embodiment, as shown in Figures 3 to 7 The second flow guide pipe 8 is a tapered pipe with a small top aperture and a large bottom aperture, which can make the cells of the sample cell liquid subjected to downward force during centrifugation, so as to facilitate the separation of the cells and the supernatant.

[0081] In one specific embodiment, as shown in Figures 3 to 7 The centrifugal processing chamber 11 is a tapered annular ring with a small top aperture and a large bottom aperture, which can make the cells of the sample cell liquid subjected to downward force during centrifugation, so as to facilitate the separation of the cells and the supernatant, and can avoid the cells being discharged with the supernatant, and improve the recovery rate.

[0082] In one specific embodiment, as shown in Figures 3 to 7As shown, the first end of the first flow guide pipe 3 is fixedly connected with the inner through hole 14 at the top of the centrifugal cup body 1, the bottom of the first flow guide pipe 3 is fixedly connected with the bottom wall of the centrifugal cup body 1, and the second end of the first flow guide pipe 3 is arranged at the circumferential edge of the bottom wall of the centrifugal cup body 1 and close to the outer edge 111 of the centrifugal processing bin 11, so that the concentrated cell liquid can be conveniently discharged from the outer edge 111 of the centrifugal processing bin 11 into the first flow guide pipe 3 through the second end of the first flow guide pipe 3, and then discharged through the first end of the first flow guide pipe 3, the first sealing cavity space 15 and the second outlet 23.

[0083] In a specific embodiment, as shown in Figures 3 to 7 , the first flow guide pipe 3 is an L-shaped cell discharge channel, which can conveniently discharge the concentrated cell liquid from the outer edge 111 of the centrifugal processing bin 11 to the second outlet 23, has a simple structure, is convenient for production and installation, and is conducive to reducing production cost and facilitating use.

[0084] In a specific embodiment, as shown in Figure 3 , Figure 7 , the first flow guide pipe 3 is an L-shaped cell discharge channel, which can conveniently discharge the concentrated cell liquid from the outer edge 111 of the centrifugal processing bin 11 to the second outlet 23, has a simple structure, is convenient for production and installation, and is conducive to reducing production cost and facilitating use.

[0085] In a specific embodiment, as shown in Figure 3 , Figure 7 , the first flow guide pipe 3 is an L-shaped cell discharge channel, which can conveniently discharge the concentrated cell liquid from the outer edge 111 of the centrifugal processing bin 11 to the second outlet 23, has a simple structure, is convenient for production and installation, and is conducive to reducing production cost and facilitating use.

[0086] In a specific embodiment, as shown in Figure 3 , Figure 7 , the first flow guide pipe 3 is an L-shaped cell discharge channel, which can conveniently discharge the concentrated cell liquid from the outer edge 111 of the centrifugal processing bin 11 to the second outlet 23, has a simple structure, is convenient for production and installation, and is conducive to reducing production cost and facilitating use.

[0087] In a specific embodiment, as shown in Figure 3 , Figure 7As shown, the side wall of the centrifugal cup body 1 comprises a first side wall 101 and a second side wall 102 arranged in the first side wall 101. The interlayer flow channel 12 is arranged between the outer surface of the second side wall 102 and the inner surface of the first side wall 101. Among them, by using the first side wall 101 and the second side wall 102, the interlayer flow channel 12 can be formed conveniently, and the production and installation are facilitated, which is beneficial to reduce the production cost.

[0088] In one specific embodiment, as shown in the drawings, Figures 3 to 7 As shown, the centrifugal cup body 1 comprises a main body shell 103 and a base 104. The top of the main body shell 103 is rotatably connected with the centrifugal cup head 2 along the longitudinal axis of the centrifugal cup, and the circumferential edge of the bottom of the main body shell 103 is sealingly and fixedly connected with the circumferential edge of the base 104. The inner wall of the main body shell 103 and the inner surface of the base 104 surround the centrifugal processing chamber 11. Among them, by using the main body shell 103 and the base 104, the centrifugal processing chamber 11 can be formed conveniently, and the production and installation are facilitated, which is beneficial to reduce the production cost.

[0089] In one specific embodiment, as shown in the drawings, Figure 2 As shown, Figures 4 to 6 As shown, the inlet 21 is connected with the second sealing space 16 and the liquid inlet passage 18 through the liquid inlet flow guide 25 arranged in the centrifugal cup head 2, which is simple in structure and convenient to use.

[0090] In one specific embodiment, as shown in the drawings, Figure 2 、 Figure 3 、 Figure 5 、 Figure 7 As shown, the supernatant outlet intermediate passage 19 is connected with the first outlet 22 through the third sealing space 17 and the supernatant flow guide 26 arranged in the centrifugal cup head 2, which is simple in structure and convenient to use.

[0091] In one specific embodiment, as shown in the drawings, Figures 2 to 7 As shown, the first end of the first flow guide pipe 3 is connected with the second outlet 23 through the first sealing space 15 and the cell liquid flow guide 27 arranged in the centrifugal cup head 2.

[0092] In one specific embodiment, as shown in the drawings, Figures 3 to 7 As shown, the first sealing space 15, the second sealing space 16 and the third sealing space 17 are all annular, which is simple in structure and convenient to use.

[0093] When the present application is used:

[0094] First, the centrifugal cup is installed in the rotating disc, the outer circular surface of the bottom wall of the centrifugal cup body 1 is attached to the rotating disc, and the centrifugal cup is clamped and fixed by the clamping jaw on the rotating disc. The motor drives the rotating disc to rotate, thereby driving the centrifugal cup to rotate and centrifuge. The upper part of the centrifugal cup has a fixed clamp that embraces the outer cylindrical surface of the centrifugal cup head 2, so that the centrifugal cup head 2 is fixed, and in the centrifugal operation condition, the centrifugal cup body 1 rotates, and the centrifugal cup head 2 remains stationary.

[0095] When the sample cell liquid to be treated is small (the sample volume is less than the maximum volume of the centrifugal cup in single operation), a certain volume of sample cell liquid is pumped from the inlet 21 of the centrifugal cup, and the sample cell liquid flows through the second sealing space 16 between the first pressure ring 42 and the second sealing ring 52, and then flows into the first flow guide pipe 3 through the liquid inlet channel 18, thereby entering the bottom of the centrifugal processing chamber 11. The air in the centrifugal cup is squeezed out of the first outlet 22, and the sample cell liquid is pumped into the centrifugal cup after the first outlet 22 is closed. Figure 5

[0096] As the centrifugal cup rotates, the sample cell liquid is subjected to centrifugal force. After the centrifugal cup rotates at high speed for a certain period of time, the cells and supernatant in the sample cell liquid are gradually stratified under the action of centrifugal force. At this time, the concentrated cells accumulate to the outer edge 111 of the centrifugal processing chamber 11, and the supernatant is located on the inner side 112 of the centrifugal processing chamber 11, as shown in Figure 6 At this time, the supernatant is first discharged, and the supernatant is sucked out of the first outlet 22, and the supernatant is sucked into the supernatant discharge intermediate channel 19 from the supernatant discharge channel 121, passes through the third sealing space 17 formed between the third pressure ring 53 and the third sealing ring 62, enters the supernatant flow guide 26, and is then discharged from the first outlet 22. During the supernatant discharge process, air is continuously sucked from the inlet 21 to compensate for the pressure in the centrifugal cup and maintain the balance of the pressure difference between the inside and outside of the centrifugal cup. During the centrifugation process, as the supernatant is continuously discharged, the longitudinal liquid level of the supernatant continuously moves to the outer side 111 of the centrifugal processing chamber 11. When the supernatant is discharged (the liquid level is monitored to reach the position of the supernatant discharge channel 121 or the first outlet 22 is monitored to no longer have liquid discharged, and air is discharged), the concentrated cell liquid is discharged, and the concentrated cell liquid is sucked out of the second outlet 23, and the concentrated cell liquid is sucked into the first flow guide pipe 3 from the second port 31 of the first flow guide pipe 3, flows through the first sealing space 15 formed between the lower part of the first sealing ring 41 and the inner side through hole 14 of the top of the centrifugal cup body 1, enters the cell liquid flow guide 27, and is then discharged from the second outlet 23 to collect the concentrated cells.

[0097] ​When concentrating large volume of cell solution in continuous flow (sample volume is larger than the maximum volume of centrifuge cup in single run), first pump the maximum volume of sample cell solution in single run into the centrifuge cup, the method is the same as the case when the sample cell solution is less than the maximum volume of centrifuge cup in single run. As the centrifuge cup rotates, the sample cell solution is subjected to centrifugal force. After the centrifuge cup rotates at high speed for a certain period of time, the cells and supernatant in the sample cell solution are gradually separated under the action of centrifugal force, at this time the concentrated cells accumulate to the outer edge 111 of the centrifugal processing chamber 11, and the supernatant is located at the inner side 112 of the centrifugal processing chamber 11. After a period of centrifugation, the first sample cell solution is completely separated, at this time continue to pump the sample cell solution from the inlet 21, and open the first outlet 22, as the sample cell solution continues to be pumped in, the pressure in the centrifuge cup rises, the separated supernatant is squeezed or pumped by the peristaltic pump to flow through the supernatant discharge intermediate passage port 19 from the supernatant discharge passage port 121, then pass through the third sealing space 17, enter the supernatant flow guide 26, and then be discharged from the first outlet 22, until all the sample cell solution is pumped into the centrifuge cup. Then the supernatant is pumped out from the first outlet 22 by suction, the remaining supernatant in the centrifuge cup is sucked into the supernatant discharge intermediate passage port 19 from the supernatant discharge passage port 121, passes through the third sealing space 17, enters the supernatant flow guide 26, and then is discharged from the first outlet 22. At this time, air is continuously sucked into the inlet 21 during the process of pumping out the supernatant, to compensate the pressure difference between the inside and outside of the centrifuge cup, and to maintain the balance of the pressure difference. During the centrifugation process, as the supernatant is continuously discharged, the longitudinal liquid level of the supernatant continuously moves to the outside 111 of the centrifugal processing chamber 11. When the supernatant is discharged (monitoring that the liquid level reaches the supernatant discharge passage port 121 position or monitoring that the first outlet 22 no longer discharges liquid, and discharges air), the concentrated cell solution is discharged, the concentrated cell solution is sucked into the first flow guide pipe 3 from the second end port 31 of the first flow guide pipe 3, flows through the first sealing space 15, enters the cell solution flow guide 27, and then is discharged from the second outlet 23 to collect the concentrated cells.

[0098] The scope of protection of the present application is not limited to the above-mentioned embodiments, and it is obvious that those skilled in the art can make various modifications and changes to the present application without departing from the scope and spirit of the present application. If these modifications and changes belong to the scope of the claims of the present application and their equivalent technologies, the intention of the present application also includes these modifications and changes.

Claims

1. A centrifuge cup for cell processing, characterized in that, include: Centrifuge cup body (1), centrifuge cup head (2), and first guide tube (3), wherein, The top of the centrifuge cup body (1) is rotatably and sealed to the centrifuge cup head (2) along the longitudinal axis of the centrifuge cup, and a centrifugation processing chamber (11) is provided inside the centrifuge cup body (1); The centrifuge cup head (2) is provided with an inlet (21), a first outlet (22) and a second outlet (23) at the top. The bottom of the centrifuge cup head (2) extends through the top of the centrifuge cup body (1) into the interior of the centrifuge cup body (1). The top of the centrifuge cup body (1) is rotatably and sealingly connected to the middle of the centrifuge cup head (2) along the longitudinal axis of the centrifuge cup. The first guide tube (3) is disposed inside the centrifuge cup (1). The first end of the first guide tube (3) is fixedly connected to the inner side of the top of the centrifuge cup (1). The second end of the first guide tube (3) is disposed at the bottom of the centrifuge processing chamber (11) and communicates with the outer edge (111) of the centrifuge processing chamber (11). The inlet (21) is connected to the centrifugal processing chamber (11); The first outlet (22) is connected to the inner side (112) of the centrifugal processing chamber (11) through the interlayer flow channel (12) in the side wall of the centrifugal cup (1); the interlayer flow channel (12) is located above the outer edge (111) of the centrifugal processing chamber (11); The second outlet (23) is connected to the outer edge (111) of the centrifugal processing chamber (11) through the first guide pipe (3).

2. The centrifuge cup for cell processing according to claim 1, characterized in that, The centrifuge cup includes a first sealing part (4), a second sealing part (5), and a third sealing part (6). The first sealing part (4), the second sealing part (5), and the third sealing part (6) are all axially disposed in the receiving cavity (13) inside the top of the centrifuge cup body (1). The first sealing part (4) is close to the bottom of the centrifuge cup head (2), and the third sealing part (6) is away from the bottom of the centrifuge cup head (2). The second sealing part (5) is located between the first sealing part (4) and the third sealing part (6). The bottom outer surface of the centrifuge cup head (2) and the top inner surface of the centrifuge cup body (1) are connected by the first sealing part (4), the second sealing part (5), and the third sealing part (6). A first sealing space (15) is formed between the bottom of the first sealing part (4) and the inner through hole (14) at the top of the centrifuge cup (1), and the second outlet (23) is connected to the first guide tube (3) through the first sealing space (15); A second sealing space (16) is formed between the top of the first sealing part (4) and the bottom of the second sealing part (5), and the inlet (21) is connected to the centrifugal processing chamber (11) through the second sealing space (16); A third sealing space (17) is formed between the top of the second sealing part (5) and the bottom of the third sealing part (6), and the first outlet (22) is connected to the interlayer flow channel (12) through the third sealing space (17).

3. The centrifuge cup for cell processing according to claim 2, characterized in that, The receiving cavity (13) includes: a first annular step (131), a second annular step (132), and a third annular step (133). The first annular step (131) is close to the bottom of the centrifuge cup head (2), the third annular step (133) is away from the bottom of the centrifuge cup head (2), the second annular step (132) is located between the first annular step (131) and the second annular step (132), the first sealing part (4) is placed in the first annular step (131), the second sealing part (5) is placed in the second annular step (132), and the third sealing part (6) is placed in the third annular step (133).

4. The centrifuge cup for cell processing according to claim 3, characterized in that, The inner diameters of the first annular step (131), the second annular step (132), and the third annular step (133) gradually increase.

5. The centrifuge cup for cell processing according to claim 3, characterized in that, The first sealing part (4) includes: a first sealing ring (41) and a first pressure ring (42); wherein, The first sealing ring (41) is disposed inside the first layer annular step (131) and sleeved on the outside of the bottom of the centrifuge cup head (2). The outer surface of the first sealing ring (41) forms a static seal with the inner surface of the first layer annular step (131), and the inner surface of the first sealing ring (41) forms a dynamic seal with the outer surface of the bottom of the centrifuge cup head (2). The bottom of the first sealing ring (41) and the inner through hole (14) form the first sealing space (15). The first pressure ring (42) is disposed on the top of the first sealing ring (41) and sleeved on the outer surface of the bottom of the centrifuge cup head (2). The top of the first pressure ring (42) and the bottom of the second sealing part (5) form a second sealing space (16). The first pressure ring (42) is pressed against the inner side of the top of the centrifuge cup body (2) to form a liquid inlet (18). The inlet (21) is connected to the centrifuge processing chamber (11) through the second sealing space (16) and the liquid inlet (18).

6. The centrifuge cup for cell processing according to claim 5, characterized in that, The second sealing part (5) includes: a second pressure ring (51), a second sealing ring (52), and a third pressure ring (53); wherein, The second pressure ring (51) is disposed inside the second layer annular step (132) and is fixedly connected to the second layer annular step (132); The second sealing ring (52) is disposed inside the second pressure ring (51) and sleeved on the outside of the bottom of the centrifuge cup head (2). The outer surface of the second sealing ring (52) forms a static seal with the inner surface of the second pressure ring (51), and the inner surface of the second sealing ring (52) forms a dynamic seal with the outer surface of the bottom of the centrifuge cup head (2). The second sealing space (16) and the liquid inlet (18) are both disposed between the top of the first pressure ring (42) and the bottom of the second sealing part (5). The third pressure ring (53) is disposed on the top of the second sealing ring (52) and the top of the second pressure ring (51), and is sleeved on the outer surface of the bottom of the centrifuge cup head (2). The top of the third pressure ring (53) and the bottom of the third sealing part (6) form the third sealing space (17). The third pressure ring (53) is pressed against the inner side of the top of the centrifuge cup body (2) to form the upper clear liquid discharge intermediate channel opening (19). The first outlet (22) is connected to the interlayer flow channel (12) through the third sealing space (17) and the upper clear liquid discharge intermediate channel opening (19).

7. The centrifuge cup for cell processing according to claim 6, characterized in that, The third sealing part (6) includes: a fourth pressure ring (61) and a third sealing ring (62); wherein, The fourth pressure ring (61) is disposed inside the third annular step (133) and is fixedly connected to the third annular step (133); The third sealing ring (62) is disposed inside the fourth pressure ring (61) and sleeved on the outside of the bottom of the centrifuge cup head (2). The outer surface of the third sealing ring (62) forms a static seal with the inner surface of the fourth pressure ring (61), and the inner surface of the third sealing ring (62) forms a dynamic seal with the outer surface of the bottom of the centrifuge cup head (2). The third sealing space (17) and the intermediate channel opening (19) for discharging supernatant are both disposed between the top of the third pressure ring (53) and the bottom of the third sealing part (6).

8. The centrifuge cup for cell processing according to claim 7, characterized in that, The centrifuge cup head (2) is provided with an annular boss (24) in the middle. The inner side of the top of the centrifuge cup body (1) is connected to the peripheral side wall of the annular boss (24) through a connector (7). The bottom surface of the connector (7) is pressed against the top surface of the fourth pressure ring (61). The bottom surface of the annular boss (24) is in contact with the top surface of the third sealing ring (62).

9. The centrifuge cup for cell processing according to claim 5, characterized in that, The centrifuge cup includes a second guide tube (8), which is disposed inside the centrifuge cup body (1) and sleeved outside the first guide tube (3). The first end of the second guide tube (8) is fixedly connected to the outer surface of the receiving cavity (13), and the second end of the second guide tube (8) is disposed near the bottom of the centrifuge processing chamber (11). The inlet (21) is connected to the centrifuge processing chamber (11) through the second sealed space (16), the liquid inlet (18), and the second guide tube (8).

10. The centrifuge cup for cell processing according to claim 9, characterized in that, The second guide tube (8) is a conical tube with a small top aperture and a large bottom aperture, and the centrifugal processing chamber (11) is a conical annular shape with a small top aperture and a large bottom aperture.

11. The centrifuge cup for cell processing according to claim 6, characterized in that, The first end of the first guide tube (3) is fixedly connected to the inner through hole (14) at the top of the centrifuge cup (1), the bottom of the first guide tube (3) is fixedly connected to the bottom wall of the centrifuge cup (1), and the second end of the first guide tube (3) is located at the circumferential edge of the bottom wall of the centrifuge cup (1) and close to the outer edge (111) of the centrifuge processing chamber (11).

12. The centrifuge cup for cell processing according to claim 11, characterized in that, The first guide tube (3) is an L-shaped cell discharge channel.

13. The centrifuge cup for cell processing according to claim 11, characterized in that, A first guide section (9) is provided on the bottom wall of the centrifuge cup (1) near the second end port (31) of the first guide tube (3).

14. The centrifuge cup for cell processing according to claim 13, characterized in that, The bottom of the interlayer flow channel (12) is provided with a discharge channel (121) that communicates with the inner side (112) of the centrifugal treatment chamber (11). The discharge channel (121) is arranged opposite to the second end port (31) of the first guide pipe (3). The top of the interlayer flow channel (12) is connected to the discharge intermediate channel (19).

15. The centrifuge cup for cell processing according to claim 14, characterized in that, A second guide section (10) is provided on the side wall of the centrifuge cup (1) near the discharge channel (121).

16. The centrifuge cup for cell processing according to claim 1, characterized in that, The sidewall of the centrifuge cup (1) includes a first sidewall (101) and a second sidewall (102) disposed within the first sidewall (101), and the interlayer flow channel (12) is disposed between the outer surface of the second sidewall (102) and the inner surface of the first sidewall (101).

17. The centrifuge cup for cell processing according to claim 1, characterized in that, The centrifuge cup body (1) includes a main shell (103) and a base (104). The top of the main shell (103) is rotatably connected to the centrifuge cup head (2) along the longitudinal axis of the centrifuge cup. The circumferential edge of the bottom of the main shell (103) is sealed and fixedly connected to the circumferential edge of the base (104). The centrifuge processing chamber (11) is surrounded between the inner wall of the main shell (103) and the inner surface of the base (104).

18. The centrifuge cup for cell processing according to claim 5, characterized in that, The inlet (21) is connected to the second sealed space (16) and the inlet channel (18) through the liquid inlet guide channel (25) provided in the centrifuge cup head (2).

19. The centrifuge cup for cell processing according to claim 6, characterized in that, The supernatant discharge intermediate channel (19) is connected to the first outlet (22) through the third sealed space (17) and the supernatant guide channel (26) provided in the centrifuge cup head (2).

20. The centrifuge cup for cell processing according to claim 2, characterized in that, The first end of the first guide tube (3) is connected to the second outlet (23) through the first sealed space (15) and the cell fluid guide channel (27) provided in the centrifuge cup head (2).

Images