Organic water-soluble fertilizer as well as preparation method and application thereof

Abstract

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of organic fertilizer, and particularly relates to an organic water-soluble fertilizer and a preparation method and application thereof. BACKGROUND

[0002] In the current field of kitchen waste treatment, three-phase separation technology can realize the preliminary separation of solid phase, liquid phase and oil phase. However, the existing mainstream treatment path usually disposes the solid phase and liquid phase components through anaerobic fermentation to produce biogas, and the produced biogas residue is disposed by incineration or thermal chemical conversion, and the biogas liquid is discharged into the urban sewage treatment system. Although this path can realize basic energy recovery, it cannot deeply develop the high-value organic components such as starch, protein and oil contained in the solid phase and liquid phase, thereby limiting the overall resource utilization efficiency.

[0003] In addition, part of the process attempts to convert the solid phase into organic fertilizer through aerobic composting technology, but the traditional aerobic composting has the following outstanding problems: first, there is a significant contradiction between the resource utilization efficiency and the treatment cost, which is specifically manifested in that the composting period is long, the treatment efficiency is low, and the deodorization cost is high; second, the disposal range has limitations, and only the solid phase can be disposed, and the liquid phase components generated in the pretreatment process cannot be synergistically utilized.

[0004] Another process path attempts to develop the liquid phase into water-soluble fertilizer, but faces the dilemma of poor product stability and blocked commercialization process. Specifically, the product has insufficient preservative performance, is easy to cause microbial resistance, and the shelf life stability is usually difficult to exceed 6 months; at the same time, there is a contradiction between the single function and the complexity of crop demand, and it is difficult to meet the actual needs of complex soil and crop systems, thereby limiting the application effect of the product. SUMMARY

[0005] In order to solve the above technical problems, the present application provides an organic water-soluble fertilizer and a preparation method and application thereof.

[0006] In a first aspect, the present application provides an organic water-soluble fertilizer, which specifically comprises the following components by weight: 70-85 parts of kitchen waste organic mother liquor, 5-10 parts of preservative synergist, 1-3 parts of stress resistance synergist, 5-10 parts of growth synergist, 0.5-1 part of dispersant, and 0.1-0.5 part of polyethylene glycol-400. The kitchen waste organic mother liquor is prepared by sequentially subjecting the solid phase and the liquid phase of kitchen waste to wet heat hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis; The preservative synergist is composed of 13-17 wt% natamycin solution, tea polyphenol and chitosan oligosaccharide in a weight ratio of 5-10:0.1-0.5:0.05-0.2; The anti-adverse synergist is composed of complex enzyme glycosides, plantain extract and gamma-aminobutyric acid in a weight ratio of 0.05-0.2:0.5-1.0:0.5-2. The growth-promoting synergist is composed of potassium fulvic acid and glucose acid in a weight ratio of 5-10:0.01-0.05.

[0007] The raw material organic mother liquor of the technical scheme provided in the application is prepared from kitchen waste solid phase and liquid phase through wet heat catalytic hydrolysis pretreatment and enzymatic hydrolysis of amylase, protease and lipase, forming a combined process of "wet heat hydrolysis + enzymatic hydrolysis". In the enzymatic hydrolysis conversion link, a multi-stage dynamic enzymatic hydrolysis system is developed, and the sequential action of amylase and protease is realized through pH-temperature coupling control, so that macromolecular organic matter is degraded into small molecular active substances. In the preservative and fresh-keeping link, a multi-element preservative system of "biological bacteriostatic agent + natural antioxidant" is constructed, so that a longer shelf life is realized without adding chemical preservatives. In the fertilizer effect improvement link, multiple bioactive biological stimulants are compounded, so that the functions of the components are synergistically enhanced, and the stability of the product in complex soil and the actual stress resistance effect of different crop types are enhanced.

[0008] Preferably, the organic water-soluble fertilizer specifically includes the following components in parts by weight: 74-80 parts of kitchen waste organic mother liquor, 6-9 parts of preservative synergist, 1.5-2.5 parts of anti-adverse synergist, 6-9 parts of growth-promoting synergist, 0.6-0.9 parts of dispersing agent, and 0.2-0.4 parts of polyethylene glycol-400.

[0009] Preferably, the preparation method of the kitchen waste organic mother liquor is as follows: after the kitchen waste is treated by impurity removal and oil phase separation, the kitchen waste solid phase and liquid phase are obtained, and then the kitchen waste solid phase and liquid phase are transported to a wet heat catalytic hydrolysis tank, 0.1-0.5 wt % of ammonium bisulfate is added, the pressure is adjusted to 1.0-1.5 MPa, 120-170℃ critical steam is transported to the wet heat hydrolysis tank, and catalytic hydrolysis pretreatment is performed at a stirring speed of 50-300 rpm for 1-2 h; the hydrolyzed slurry is transported to an enzymatic hydrolysis tank, the pH is adjusted to 4.5-5.5, then 0.01-0.03 wt % of amylase is added, and reaction is performed at 45-55℃ for 3-5 h; then the pH is adjusted to 6.0-8.0, 0.004-0.008 wt % of protease is added, and reaction is performed at 55-60℃ for 3-5 h; then the pH is adjusted to 7.5-8.5, 0.002-0.005 wt % of lipase is added, and reaction is performed at 40-50℃ for 3-5 h, to obtain an enzymatic hydrolysis product; the enzymatic hydrolysis product is filtered through a 200-300 mesh microfilter to remove large-particle-size solid matter, and a small-molecule organic mother liquor is obtained.

[0010] Preferably, the preservative synergist is composed of 13-17wt% natamycin solution, tea polyphenol and chitooligosaccharide in a weight ratio of 6-9:0.2-0.4:0.1-0.15.

[0011] In a specific embodiment, the weight ratio of 13-17wt% natamycin solution, tea polyphenol and chitooligosaccharide in the preservative synergist can be 5:0.1:0.05, 6:0.1:0.05, 7:0.1:0.05, 9:0.1:0.05, 10:0.1:0.05, 5:0.2:0.05, 6:0.3:0.05, 7:0.4:0.05, 9:0.5:0.05, 5:0.1:0.1, 6:0.1:0.12, 7:0.1:0.15, 9:0.1:0.2.

[0012] Through experimental analysis, it is found that the preservative synergist composed of 13-17wt% natamycin solution, tea polyphenol and chitooligosaccharide in the above weight ratio can further improve the stability of the organic water-soluble fertilizer.

[0013] Preferably, the stress resistance synergist is composed of complex glycoside, plantain extract and gamma-aminobutyric acid in a weight ratio of 0.1-0.15:0.6-0.9:1-1.5.

[0014] In a specific embodiment, the weight ratio of complex glycoside, plantain extract and gamma-aminobutyric acid in the stress resistance synergist can be 0.05:0.5:0.5, 0.1:0.5:0.5, 0.12:0.5:0.5, 0.15:0.5:0.5, 0.2:0.5:0.5, 0.05:0.6:0.5, 0.1:0.7:0.5, 0.12:0.9:0.5, 0.15:1.0:0.5 0.05:0.5:1, 0.1:0.5:1.2, 0.12:0.5:1.5, 0.15:0.5:2.

[0015] Through experimental analysis, it is found that the stress resistance synergist composed of complex glycoside, plantain extract and gamma-aminobutyric acid in the above weight ratio can further improve the stability of the organic water-soluble fertilizer.

[0016] Preferably, the stress resistance synergist is composed of complex glycoside, plantain extract and gamma-aminobutyric acid in a weight ratio of 0.1-0.15:0.6-0.9:1-1.5.

[0017] In one specific implementation, the weight ratio of potassium humate and gluconic acid in the growth promoter can be 5:0.01, 6:0.01, 7:0.01, 9:0.01, 10:0.01, 5:0.02, 6:0.02, 7:0.02, 9:0.03, 10:0.04, 5:0.03, 6:0.04, 7:0.05, 9:0.03, or 10:0.04.

[0018] Experimental analysis shows that the above-mentioned weight ratio of mineral-derived potassium humate and gluconic acid, used in this application to form a growth promoter and enhancer, can further improve the growth-promoting performance of organic water-soluble fertilizers.

[0019] Preferably, the dispersant is polyvinylpyrrolidone.

[0020] Secondly, this application provides a method for preparing the above-mentioned organic water-soluble fertilizer, which specifically includes the following steps in sequence: weighing the corresponding weights of each raw material component, mixing them evenly, and thus obtaining the fertilizer.

[0021] Thirdly, this application provides the application of the aforementioned organic water-soluble fertilizer in crop cultivation.

[0022] Preferably, the crop is any one of the following: tomato, pepper, radish, carnation, bok choy, cucumber, and melon.

[0023] In summary, the technical solution of this application has the following effects: This technology successfully solved the following key challenges through systematic process optimization and innovative design of functional components: (1) The combined process of "wet heat catalytic hydrolysis and enzymatic hydrolysis" realizes the efficient resource utilization of kitchen waste. Hydraulic catalytic hydrolysis can break down the complex organic solid structures (such as plant cell walls) in food waste, denature proteins, and gelatinize starches, thereby significantly improving the efficiency of subsequent enzymatic hydrolysis. During the enzymatic hydrolysis stage, a multi-stage dynamic enzymatic hydrolysis technology is employed. By precisely controlling the pH gradient, temperature fluctuations, and the synergistic effect of complex enzyme systems, the directional pyrolysis efficiency of macromolecular organic matter such as starch, protein, and fat in the liquid phase of food waste is significantly improved. Simultaneously, the large-scale production of high-purity water-soluble organic matter (such as oligosaccharides, small peptides, amino acids, and fatty acids) is achieved, providing a stable source of raw materials for the core components of liquid fertilizer and reducing resource waste and the risk of secondary pollution.

[0024] (2) Construct an environmentally friendly anti-corrosion and component stabilization system Due to the rich variety of organic components, small molecule organic mother liquor is particularly prone to souring. The added natamycin, tea polyphenols and chitosan oligosaccharide can inhibit and kill bacteria through multiple targets: ① Fungal inhibition: natamycin inhibits mold and yeast, tea polyphenols inhibit bacteria and oxidation, and chitosan oligosaccharide forms a physical barrier and activates plant resistance, all of which block the souring path from different angles; ② Dual control of bacteria and oxidation: tea polyphenols inhibit bacterial reproduction and lipid oxidation, chitosan oligosaccharide reduces oxygen permeation, and natamycin prevents fungal metabolic acid production. Through the synergistic effect of multi-target inhibition, physical barrier formation, antioxidant protection and immune activation, natamycin, tea polyphenols and chitosan oligosaccharide effectively inhibit the souring problem of organic water-soluble fertilizer.

[0025] The core component of the organic water-soluble fertilizer is obtained by wet heat catalytic hydrolysis, and the system will contain small particles. By adding polyvinylpyrrolidone as a dispersant, the organic particles are dispersed, and through steric hindrance effect and electrostatic repulsion, the particles are uniformly dispersed in the solution, preventing them from aggregating and precipitating, thereby maintaining the stability and uniformity of the fertilizer solution. Stable suspension system: For some liquid fertilizers containing insoluble solid components (such as poorly soluble salts of trace elements), polyvinylpyrrolidone can reduce the surface tension between the particles and the liquid, making it easier for the liquid to wet the particles, and forming a protective barrier around the particles, allowing these solid particles to be stably suspended in the solution, forming a stable suspension system.

[0026] (3) Synergistic enhancement of components, metabolic complementation, and strengthening of the actual use effect of organic water-soluble fertilizer The signal transduction of complex glycoside and gamma-aminobutyric acid has a synergistic effect, enhancing the complex resistance of plants to salt, alkali, drought and other multiple stresses; the antioxidant components of plantain combined with the osmoregulation function of gamma-aminobutyric acid reduce the accumulation of reactive oxygen species under stress and protect the structure of cell membranes. Complex glycoside promotes plant carbon metabolism, plantain extract provides an antioxidant barrier, and gamma-aminobutyric acid regulates energy distribution, promoting metabolic complementation in crops. Through the triple mechanism of enzyme activation-signal transduction-antioxidation, the actual application effect is strengthened.

[0027] Natamycin, tea polyphenols and chitosan oligosaccharide not only inhibit the souring of organic water-soluble fertilizer, but also inhibit fungal and bacterial diseases in the actual application environment of crops. Specifically, ① enhanced permeability: chitosan oligosaccharide increases the permeability of bacterial cell membranes, promoting the penetration of tea polyphenols into the interior and blocking bacterial growth; ② broad-spectrum coverage: natamycin and tea polyphenols can simultaneously inhibit multiple fungi and bacteria; ③ improved effect stability: the film-forming property of chitosan oligosaccharide can reduce the loss of natamycin and tea polyphenols, and the antioxidant property of tea polyphenols protects natamycin from degradation.

[0028] The added gluconic acid in the component is a polyhydroxy carboxylic acid with strong metal ion chelating ability, which can effectively dissolve the insoluble Fe³ + , Ca² + , Mg² + ions in the soil and improve their bioavailability. At the same time, it can form more stable organic-metal complexes with the potassium source fulvic acid in the component, prolonging the effective time of nutrients in the rhizosphere. In addition, under salt stress or drought conditions, the potassium source fulvic acid can adjust the osmotic pressure of plants, enhance the activity of antioxidant enzymes, and synergize with complex glycosides, plantain extract, and gamma-aminobutyric acid. Gluconic acid can act as an important medium in the energy conversion process in living organisms, participate in a series of important metabolic processes such as energy and acid-base balance, and provide an organic carbon framework through the tricarboxylic acid cycle to link carbon and nitrogen metabolism, thereby improving the physiological functions of crops.

[0029] (4) After the organic water-soluble fertilizer prepared by the present application is used in field experiments, it is beneficial to the growth of the above-ground parts and roots of crops, has a good growth-promoting effect on crops such as tomatoes and peppers, can reduce the number of continuous cropping obstacle diseased plants, and can improve the yield of crops in continuous cropping obstacle soil environments, which has good significance in agricultural production. DETAILED DESCRIPTION

[0030] The present application will be further described in detail below in combination with examples, comparative examples, and performance test experiments, and these examples should not be understood as limiting the scope of the present application.

[0031] The raw materials used in the present application can be obtained from the market; among them, ammonium bisulfate is purchased from Zibo Yu Jin Trading Co., Ltd.; 15% natamycin is purchased from Shandong Weiguan Biological Technology Co., Ltd.; complex glycosides are purchased from Yantai Guteli Biological Technology Co., Ltd.; chitosan is purchased from Shandong Weikang Biological Medicine Technology Co., Ltd.; gamma-aminobutyric acid is purchased from Nanning Hanhe Biological Technology Co., Ltd.; plantain extract is purchased from Lanzhou Waterless Biological Technology Co., Ltd.; potassium source fulvic acid is purchased from Shandong Innovation Humic Acid Technology Co., Ltd.; amylase, protease, and lipase are provided by Jiabowen Biological Technology Co., Ltd. BRIEF DESCRIPTION OF DRAWINGS

[0032] Figure 1 The process flow diagram for the preparation method of the organic mother liquor of kitchen waste in Preparation Example 1.

[0033] Figure 2 The above-ground growth potential diagram of tomatoes after 40 days of flush treatment under normal soil conditions; a is the control group, and b is the group of Example 1.

[0034] Figure 3Figure 1 is a diagram showing the growth of the root system of tomatoes after 40 days of the flush treatment under normal soil conditions; a is the control group, and b is the group of Example 1.

[0035] Figure 4 Figure 2 is a diagram showing the growth of the above-ground part of peppers after 35 days of the flush treatment under the condition of continuous cropping obstacles; a is the control group, and b is the group of Example 1.

[0036] Figure 5 Figure 3 is a diagram showing the growth of the root system of peppers after 35 days of the flush treatment under the condition of continuous cropping obstacles; a is the control group, and b is the group of Example 1.

[0037] Figure 6 Figure 4 is a diagram showing the growth of the above-ground part of eggplants after 25 days of the flush treatment under the condition of saline-alkali soil; a is the control group, and b is the group of Example 1.

[0038] Preparation Example Preparation Example 1 The present preparation example provides a kitchen waste organic mother liquor and a preparation method thereof.

[0039] Figure 1 Figure 5 is a schematic diagram of the process flow of the preparation method of the kitchen waste organic mother liquor in Preparation Example 1; the preparation method specifically comprises the following steps: after the impurity removal and oil phase separation treatment, the solid phase and the liquid phase of the kitchen waste are obtained and transported to a tank of a wet heat catalytic hydrolysis system, 0.25wt% ammonium bisulfate is added, the pressure is adjusted to 1.2MPa, 150℃ critical steam is transported to the wet heat hydrolysis tank, and catalytic hydrolysis is performed at a stirring speed of 100rpm for 1.5h; the hydrolyzed slurry is transported to an enzymatic hydrolysis tank of a biological enzymatic hydrolysis system, the pH is adjusted to 5.0, then 0.02wt% amylase is added, and reaction is performed at 50℃ for 4h; then the pH is adjusted to 7.0, 0.006wt% protease is added, and reaction is performed at 55℃ for 4h; then the pH is adjusted to 8.0, 0.003wt% lipase is added, and reaction is performed at 45℃ for 4h, to obtain an enzymatic hydrolysis product; the enzymatic hydrolysis product enters a filtration separation system, is filtered through a 200-300 mesh microfilter to remove large-particle-size solid substances, and small-molecule organic mother liquor is obtained, which is the kitchen waste organic mother liquor.

[0040] It is detected that the organic matter content (detection method NY / T1976-2010) in the kitchen waste organic mother liquor is 12.67wt%, the free amino acid content (NY / T1975-2010) is 2.83wt%, the hydrolyzed amino acid (GB / T-18246-2019) is 6.91wt%, and the small peptide content (GB / T-22492-2008) is 3.15wt%.

[0041] Preparation Example 2 The present preparation example provides a kitchen waste organic mother liquor and a preparation method thereof.

[0042] The preparation method of the organic mother liquor of the kitchen waste in the preparation example is as follows: after impurity removal and oil phase separation treatment, the solid phase and the liquid phase of the kitchen waste are obtained, and are transported to a tank of a wet heat catalytic hydrolysis system, 0.25wt% ammonium bisulfate is added, the pressure is adjusted to 1.2MPa, 150℃ critical steam is transported to the wet heat hydrolysis tank, and catalytic hydrolysis is carried out at a stirring speed of 100rpm for 1.5h; the hydrolysis product enters a filtration separation system, is filtered by a 200-300 mesh microfilter to remove large particle size solid, and small molecule organic mother liquor is obtained, which is the organic mother liquor of the kitchen waste.

[0043] It is detected that the organic mother liquor of the kitchen waste contains 10.15wt% organic matter, 2.06wt% free amino acid, 5.11wt% hydrolyzed amino acid and 2.73wt% small peptide.

[0044] Preparation Example 3 The preparation example provides an organic mother liquor of kitchen waste and a preparation method thereof.

[0045] The preparation method of the organic mother liquor of the kitchen waste in the preparation example is as follows: after impurity removal and oil phase separation treatment, the solid phase and the liquid phase of the kitchen waste are obtained, and are transported to an enzymolysis tank of a biological enzymolysis system, the pH is adjusted to 5.0, then 0.02wt% amylase is added, and reaction is carried out at 50℃ for 4h; then the pH is adjusted to 7.0, 0.006wt% protease is added, and reaction is carried out at 55℃ for 4h; then the pH is adjusted to 8.0, 0.003wt% lipase is added, and reaction is carried out at 45℃ for 4h, to obtain an enzymolysis product; the enzymolysis product enters a filtration separation system, is filtered by a 200-300 mesh microfilter to remove large particle size solid, and small molecule organic mother liquor is obtained, which is the organic mother liquor of the kitchen waste.

[0046] It is detected that the organic mother liquor of the kitchen waste contains 11.28wt% organic matter, 2.54wt% free amino acid, 6.12wt% hydrolyzed amino acid and 2.69wt% small peptide.

[0047] By comparing the organic mother liquor of kitchen waste prepared in preparation example 1-3, it can be seen that the organic mother liquor of kitchen waste prepared by only wet heat hydrolysis or only enzymatic hydrolysis of the solid phase and liquid phase of kitchen waste in preparation example 2-3 has a lower content of organic matter and a lower content of nutrients such as amino acids and small peptides. The reason is that only hydrolysis or enzymatic hydrolysis is not sufficient to fully and deeply convert macromolecular organic matter. Due to the filtering mechanism of the microfiltration machine, part of the organic nutrient components are intercepted and thus cannot enter the organic mother liquor. In contrast, the organic mother liquor of kitchen waste prepared by the method of first wet heat catalytic hydrolysis and then enzymatic hydrolysis of the solid phase and liquid phase of kitchen waste in preparation example 1 of the present application has a higher content of organic matter, amino acids and small peptides. Therefore, in the process of preparing the organic water-soluble fertilizer, the organic mother liquor of kitchen waste prepared in preparation example 1 is used as the raw material.

[0048] Embodiment

[0049] Embodiments 1-3 Embodiments 1-3 respectively provide an organic water-soluble fertilizer and a preparation method and application thereof.

[0050] The difference between the above embodiments is that the amount of each component in the organic water-soluble fertilizer is different, as shown in Table 1.

[0051] The preservative synergist is composed of 15wt% natamycin solution, tea polyphenol and chitosan oligosaccharide with a weight ratio of 7:0.3:0.12.

[0052] The stress resistance synergist is composed of complex glycoside, plantain extract and gamma-aminobutyric acid with a weight ratio of 0.12:0.7:1.2.

[0053] The growth promoting synergist is composed of mineral potassium fulvic acid and glucose acid with a weight ratio of 7:0.03.

[0054] According to Table 1, the corresponding weights of each raw material component of the organic mother liquor of kitchen waste (prepared in preparation example 1), preservative synergist, stress resistance synergist, growth promoting synergist, dispersant polyvinylpyrrolidone and polyethylene glycol-400 are respectively weighed, and then mixed uniformly. After passing through the filling and dosing system, the finished product is obtained.

[0055] Table 1 Amount of each component in the organic water-soluble fertilizer in embodiments 1-3 and comparative examples 1-2

[0056] Embodiments 4-9 Embodiments 4-9 respectively provide an organic water-soluble fertilizer and a preparation method thereof.

[0057] The difference between the above embodiment and embodiment 1 is as follows.

[0058] In example 4: the preservative synergist is composed of 15wt% natamycin solution, tea polyphenol and chitooligosaccharide with a weight ratio of 5:0.5:0.05.

[0059] In example 5: the preservative synergist is composed of 15wt% natamycin solution, tea polyphenol and chitooligosaccharide with a weight ratio of 10:0.1:0.2.

[0060] In example 6: the stress resistance synergist is composed of complex enzyme glycoside, plantain extract and gamma-aminobutyric acid with a weight ratio of 0.05:1.0:0.5.

[0061] In example 7: the stress resistance synergist is composed of complex enzyme glycoside, plantain extract and gamma-aminobutyric acid with a weight ratio of 0.2:0.5:2.

[0062] In example 8: the growth promotion synergist is composed of potassium fulvic acid and glucose acid with a weight ratio of 5:0.05.

[0063] In example 9: the growth promotion synergist is composed of potassium fulvic acid and glucose acid with a weight ratio of 10:0.01.

[0064] In the above examples, other process parameters are the same as those in example 1.

[0065] Comparative example Comparative example 1-2 Comparative example 1-2 respectively provides an organic water-soluble fertilizer and a preparation method thereof.

[0066] The difference between the above comparative example and example 1 is that the amount of each component in the organic water-soluble fertilizer is different, as shown in Table 1.

[0067] In the above comparative example, other process parameters are the same as those in example 1.

[0068] Comparative example 3-7 Comparative example 3-7 respectively provides an organic water-soluble fertilizer and a preparation method thereof.

[0069] The difference between the above comparative example and example 1 is as follows.

[0070] In comparative example 3: in the preservative synergist, an equal amount of 15wt% caspofungin solution is used instead of 15wt% natamycin solution.

[0071] In comparative example 4: the preservative synergist is composed of 15wt% natamycin solution, tea polyphenol and chitooligosaccharide with a weight ratio of 7:0.05:0.5.

[0072] In Comparative Example 5: In the anti-stress synergist, an equal amount of chitosan oligosaccharide was used to replace the complex enzyme glycoside.

[0073] In Comparative Example 6: the stress-relieving synergist was composed of a mixture of complex enzyme glycosides, plantain extract and γ-aminobutyric acid in a weight ratio of 0.01:2:0.1.

[0074] In Comparative Example 7: the growth promoter was composed of a mixture of mineral-derived potassium humate and gluconic acid in a weight ratio of 3:0.1.

[0075] All other process parameters in the above comparative examples are the same as those in Example 1.

[0076] Performance testing (1) Performance of organic water-soluble fertilizers Organic matter: The organic matter in organic water-soluble fertilizers was tested according to the test methods specified in NY / T1976-2010 "Determination of Organic Matter Content in Water-Soluble Fertilizers".

[0077] Bacterial count: The bacterial count was determined according to the detection method specified in NY / T2321-2013.

[0078] Product stability: Organic water-soluble fertilizers were placed at room temperature for 6 months and 12 months, and then the organic matter and number of miscellaneous bacteria in the organic water-soluble fertilizers were tested.

[0079] Test results are shown in Table 2.

[0080] Table 2 Performance test results of organic water-soluble fertilizers in the examples and comparative examples

[0081] As can be seen from the test results in the table above, the organic water-soluble fertilizer prepared using the technical solution provided in this application has a high content of organic matter and a low rate of miscellaneous bacteria, indicating that the fertilizer has good anti-corrosion performance; and the organic water-soluble fertilizer product prepared by this technical solution has high stability.

[0082] By comparing the test results of Examples 1-3 and Comparative Examples 1-2, it can be seen that the dosage of each raw material component has a significant impact on the product performance. The dosages of each raw material component in Comparative Examples 1-2 are mismatched; the organic water-soluble fertilizer prepared in Comparative Example 1 has a lower organic matter content, while the organic water-soluble fertilizer prepared in Comparative Example 2 has poorer anti-corrosion performance and product stability. In contrast, this application selects specific dosages of organic mother liquor from kitchen waste, anti-corrosion synergists, stress-resistant synergists, growth-promoting synergists, dispersants, and polyethylene glycol-400 as raw materials, resulting in an organic water-soluble fertilizer with higher organic matter content and excellent anti-corrosion performance and product stability.

[0083] From the detection results of comparative examples 1, 4-5, and comparative examples 3-4, it can be seen that in comparative example 3, the preservative synergist is replaced by 15wt% caspofungin solution instead of 15wt% natamycin solution in equal amount, and in comparative example 4, the preservative synergist is composed of 15wt% natamycin solution, tea polyphenol, and chitosan oligosaccharide in a weight ratio of 7:0.05:0.5, and the preservative performance and product stability of the organic water-soluble fertilizer prepared are poor. In contrast, the present application selects to use a preservative synergist composed of 13-17wt% natamycin solution, tea polyphenol, and chitosan oligosaccharide in a weight ratio of 5-10:0.1-0.5:0.05-0.2, and the organic water-soluble fertilizer prepared has excellent performance.

[0084] From the detection results of comparative examples 1, 6-7, and comparative examples 5-6, it can be seen that in comparative example 5, the stress resistance synergist is replaced by chitosan oligosaccharide instead of complex glycoside in equal amount, and in comparative example 6, the stress resistance synergist is composed of complex glycoside, plantain extract, and gamma-aminobutyric acid in a weight ratio of 0.01:2:0.1, and the preservative performance and product stability of the organic water-soluble fertilizer prepared are poor. In contrast, the present application selects to use a stress resistance synergist composed of complex glycoside, plantain extract, and gamma-aminobutyric acid in a weight ratio of 0.05-0.2:0.5-1.0:0.5-2, and the organic water-soluble fertilizer prepared has excellent performance.

[0085] From the detection results of comparative examples 1, 8-9, and comparative example 7, it can be seen that in comparative example 7, the growth promotion synergist is composed of potassium-mineral fulvic acid and gluconic acid in a weight ratio of 3:0.1, and the preservative performance and product stability of the organic water-soluble fertilizer prepared are poor. In contrast, the present application selects to use a growth promotion synergist composed of potassium-mineral fulvic acid and gluconic acid in a weight ratio of 5-10:0.01-0.05, and the organic water-soluble fertilizer prepared has excellent performance.

[0086] (2) Effect of organic water-soluble fertilizer on growth and development of tomato under normal soil conditions The test was carried out under greenhouse conditions by using potting method. The test soil was normal garden soil, and 5.5 kg of soil was placed in each pot. The base fertilizer was balanced compound fertilizer (NPK 15-15-15), and the application amount was 5 g / pot. After mixing with the soil, the pot was filled. One uniform tomato seedling was planted in each pot, and 4 replicates were set for each treatment.

[0087] For the fertilizer treatment, the organic water-soluble fertilizer was diluted 100 times to prepare a solution for flushing treatment, and the application amount was 10 mL / pot each time. The control treatment was flushed with an equal amount of water per pot. The first flushing was performed at the time of tomato planting, and then it was flushed every 7 days, a total of 5 times during the whole growth period.

[0088] As Figure 2The above is the above-ground growth vigor chart of tomato 40 days after planting under normal soil conditions; a is the control group, b is the example 1 group; as Figure 3 The above is the above-ground growth vigor chart of tomato 40 days after planting under normal soil conditions; a is the control group, b is the example 1 group; as Figure 2 and Figure 3 It can be seen that, compared with the control group (a) treated with water, the treatment group (b) using the organic water-soluble fertilizer prepared in example 1 of the present application is significantly better than the control group in terms of above-ground and root growth vigor of tomato, indicating that the fertilizer has obvious promoting effect on the growth of tomato.

[0089] After 40 days of tomato planting, the above-ground fresh weight, plant height, single fruit weight and root dry weight were counted, and the specific data are shown in Table 3.

[0090] Table 3 Influence of organic water-soluble fertilizer in example on growth and development of tomato

[0091] As shown in Table 3, compared with the control group and the comparative example, the organic water-soluble fertilizer provided by the present application significantly improves the above-ground fresh weight, plant height and root dry weight of tomato, and can increase the fruit setting number and total fruit weight per plant, proving that the organic water-soluble fertilizer prepared in the present application has obvious growth promoting effect on tomato.

[0092] (3) Influence of organic water-soluble fertilizer on growth and development of pepper under continuous cropping obstacle soil conditions In this test, greenhouse potting method was used, and the test soil was taken from the field where pepper had been continuously planted for many years and continuous cropping obstacles had appeared. 5.5 kg of soil was placed in each pot, and 5 g of balanced compound fertilizer (NPK 15-15-15) was used as basal fertilizer, which was mixed with soil and then placed in the pot. Pepper seedlings with consistent growth vigor were selected, and 1 plant was planted in each pot, and 5 replicates were set for each treatment.

[0093] In terms of fertilizer management, the organic water-soluble fertilizer was diluted 100 times to prepare a solution for flushing treatment, and the amount of each application was 10 mL / pot. The control treatment was flushed with an equal amount of water per pot. The first flushing was performed at the time of pepper planting, and then every 7 days, a total of 4 times during the whole growth period.

[0094] Figure 4 The above is the above-ground growth vigor chart of tomato 40 days after planting under normal soil conditions; a is the control group, b is the example 1 group; as Figure 5 The above is the above-ground growth vigor chart of tomato 40 days after planting under normal soil conditions; a is the control group, b is the example 1 group; as

[0095] From Figure 4 and Figure 5It can be seen directly that compared with the control group treated with clean water, the pepper treated with the organic water-soluble fertilizer prepared in the application has more vigorous aboveground part, more root system and whiter root system, indicating that the root system is healthier.

[0096] After the pepper was planted for 35 days, the stem diameter, plant height, yield, root weight and total weight of the aboveground part of the pepper were counted, and the data results are shown in Table 4.

[0097] Table 4 Influence of the organic water-soluble fertilizer in the example on the growth and development of the pepper

[0098] As can be seen from Table 4, compared with the control group treated with clean water, the pepper treated with the example 1 has an increase of 4.74%, 11.28% and 11.66% in stem diameter, plant height and root weight respectively; the total weight of the aboveground part and the yield per plant are increased by 21.76% and 24.73% respectively, which indicates that the organic water-soluble fertilizer prepared in the application can promote the growth of the aboveground part and root system of the pepper under the condition of continuous cropping obstacle soil and increase the yield of the pepper.

[0099] (4) Influence of the organic water-soluble fertilizer on the growth and development of eggplant under saline-alkali soil condition In the experiment, a greenhouse potting method was used, the test soil was collected from a saline-alkali land in Yancheng beach (soil pH 8.47, total salt content 0.38%), which belongs to a mild saline-alkali land; 5.5 kg of soil was loaded in each pot, 5 g of balanced compound fertilizer (NPK 15-15-15) was used as basal fertilizer, and the fertilizer and soil were fully mixed before potting. Eggplant seedlings with consistent growth were selected, one plant was planted in each pot, and four replicates were set for each treatment.

[0100] In terms of fertilization management, the organic water-soluble fertilizer was diluted 50 times to prepare a solution for flushing treatment, and the amount of each application was 10 mL / pot. The control treatment was flushed with an equal amount of clean water per pot. The first flushing was performed when the eggplant was planted, and then the flushing was performed every 7 days, and a total of 3 times were performed during the whole growth period.

[0101] Figure 6 The aboveground part growth vigor diagram of the eggplant after 25 days of flushing treatment under the saline-alkali soil condition; a is the control group, and b is the example 1 group. It can be seen that the aboveground part growth vigor of the example 1 group is obviously better than that of the control group.

[0102] After planting for 25 days, the stem diameter, plant height and fresh weight of the aboveground part of the eggplant were counted, and the data results are shown in Table 5.

[0103] Table 5 Influence of the organic water-soluble fertilizer in the example on the growth and development of the eggplant

[0104] As shown in Table 5, compared with the control group treated with clean water, the eggplant treated with the example 1 has the stem diameter, plant height and fresh weight of the above ground part increased by 11.6%, 19.7% and 30.1% respectively; according to Figure 6 The results in Table 5 can show that the organic water-soluble fertilizer prepared in the application can promote the above ground part growth of eggplant in the light saline-alkali soil.

[0105] Although the present application has been described in detail with general description and specific embodiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application claimed.

Claims

1. An organic water-soluble fertilizer, characterized in that, Specifically, it includes the following components by weight: 70-85 parts organic mother liquor from kitchen waste, 5-10 parts preservative synergist, 1-3 parts stress-resistant synergist, 5-10 parts growth-promoting synergist, 0.5-1 part dispersant, and 0.1-0.5 parts polyethylene glycol-400; The organic mother liquor from kitchen waste is prepared by sequentially hydrolyzing the solid and liquid phases of kitchen waste through wet hot water hydrolysis, amylase hydrolysis, protease hydrolysis, and lipase hydrolysis. The corrosion inhibitor is prepared at a weight ratio of 5-10: Composed of 0.1-0.5:0.05-0.2% 13-17wt% natamycin solution, tea polyphenols, and chitosan oligosaccharides; The stress-relieving synergist is composed of a mixture of complex enzyme glycosides, plantain extract, and γ-aminobutyric acid in a weight ratio of 0.05-0.2:0.5-1.0:0.5-2. The growth promoter and synergist is composed of a weight ratio of 5-10: It is composed of 0.01-0.05% mineral-derived potassium humate and gluconic acid.

2. The organic water-soluble fertilizer according to claim 1, characterized in that, Specifically, it includes the following components by weight: 74-80 parts organic mother liquor from kitchen waste, 6-9 parts preservative synergist, 1.5-2.5 parts stress-resistant synergist, 6-9 parts growth-promoting synergist, 0.6-0.9 parts dispersant, and 0.2-0.4 parts polyethylene glycol-400.

3. The organic water-soluble fertilizer according to claim 1, characterized in that, The method for preparing the organic mother liquor from kitchen waste is as follows: the solid and liquid phases of the kitchen waste after impurity removal and three-phase separation are transported to a wet-heat catalytic hydrolysis tank, and 0.1-0.5 wt% of [unspecified ingredient] is added. Pretreatment is performed by catalytic hydrolysis of ammonium bisulfate at a pressure of 1.0-1.5 MPa under 120-170℃ critical steam for 1-2 hours with stirring at 50-300 rpm. The hydrolyzed slurry is then transferred to an enzymatic hydrolysis tank, where the pH is adjusted to 4.5-5.

5. 0.01-0.03 wt% amylase is added, and the mixture is reacted at 45-55℃ for 3-5 hours. The pH is then adjusted to 6.0-8.0, and 0.004-0.008 wt% protease is added, reacting at 55-60℃ for 3-5 hours. Finally, the pH is adjusted to 7.5-8.5, and 0.002-0.005 wt% lipase is added, reacting at 40-50℃ for 3-5 hours to obtain the enzymatic hydrolysis product. This product is then filtered through a 200-300 mesh microfilter to remove large-particle solids, yielding a small-molecule organic mother liquor.

4. The organic water-soluble fertilizer according to claim 1, characterized in that, The preservative synergist is composed of a 13-17wt% natamycin solution, tea polyphenols, and chitosan oligosaccharides in a weight ratio of 6-9:0.2-0.4:0.1-0.

15.

5. The organic water-soluble fertilizer according to claim 1, characterized in that, The stress-relieving synergist is composed of a mixture of complex enzyme glycosides, plantain extract, and γ-aminobutyric acid in a weight ratio of 0.1-0.15:0.6-0.9:1-1.

5.

6. The organic water-soluble fertilizer according to claim 1, characterized in that, The growth promoter is composed of a mixture of mineral-derived potassium humate and gluconic acid in a weight ratio of 6-9:0.02-0.

04.

7. The organic water-soluble fertilizer according to claim 1, characterized in that, The dispersant is polyvinylpyrrolidone.

8. A method for preparing an organic water-soluble fertilizer as described in any one of claims 1-7, characterized in that, Specifically, the process includes the following steps: weighing the corresponding weights of each raw material component, mixing them evenly, and then obtaining the final product.

9. The application of an organic water-soluble fertilizer as described in any one of claims 1-7 in crop cultivation.

10. The application of the organic water-soluble fertilizer according to claim 9 in crop cultivation, characterized in that, The crop mentioned is any one of the following: tomato, pepper, radish, carnation, bok choy, cucumber, and melon.

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