Method for electrochemically detecting sugar concentration

Abstract

The invention provides a method for electrochemically detecting sugar concentration. The method comprises the following steps: preparing a solution to be detected, detecting and analyzing. And a to-be-detected solution preparation step: mixing the sugar-containing to-be-detected sample with the copper sulfate-containing solvent to obtain the to-be-detected solution. And a detection step: dropwise adding the solution to be detected into the electrode test piece in a room temperature environment without additional heating, applying a driving voltage to the electrode test piece through an electrochemical voltammetry method so as to activate an oxidation-reduction reaction of the solution to be detected, and monitoring a current value of the electrode test piece in a potential section so as to obtain current value data. And an analysis step: comparing the sugar potential standard data with the current value data to obtain sugar concentration data of the sugar-containing sample to be detected. The method has the advantages of low operation difficulty, high detection efficiency and high detection precision, and solves the production safety problem caused by an old heating program.

Description

Technical Field

[0001] This invention relates to a detection technology, and more particularly to an electrochemical method for detecting sugar concentration. Background Technology

[0002] As is well known, many methods for detecting sugar concentration in liquids have been proposed in general industries, such as optical detection methods (including refractometer method and spectrophotometer method) that detect sugar concentration through optical principles (light absorption or refractive index change), and Benedict's solution detection method that detects sugar concentration through redox reactions, etc.

[0003] In optical detection methods, the test solutions need to be prepared by the participation of enzymes (enzymes) in the reaction. During the test, the absorption of light or the change of refractive index is easily affected by the color of the test solution, resulting in existing optical detection methods having problems such as high operation difficulty and poor accuracy.

[0004] Furthermore, in the Benn's solution detection method, a heating procedure is required in each detection step, and the approximate sugar concentration of the test solution can only be determined by color change. This results in the existing Benn's solution detection method having problems such as low detection accuracy and the heating process being prone to danger. Summary of the Invention

[0005] The main objective of this invention is to solve the problems of high operational difficulty and poor accuracy of existing optical detection methods, as well as the problems of low detection accuracy and potential danger during the heating process of existing Benedictine liquid detection methods.

[0006] To achieve the above objectives, one embodiment of the present invention provides a method for electrochemical detection of sugar concentration, including a test solution preparation step, a detection step, and an analysis step. The test solution preparation step involves mixing a sugar-containing test sample with a copper sulfate-containing solvent to form a test solution. The detection step involves adding the test solution to an electrode plate at room temperature without additional heating, and applying a driving voltage to the electrode plate using electrochemical voltammetry to activate the redox reaction of the test solution. The current value of the electrode plate is monitored in a potential range to obtain current value data. The analysis step involves comparing the sugar potential standard data and current value data in a standard database to obtain the sugar concentration data of the sugar-containing test sample.

[0007] In another embodiment of the present invention, the volume of the sugar-containing test sample is 0.1 ml to 1 ml.

[0008] In another embodiment of the present invention, the detection time of the electrochemical voltammetry is less than 1 minute.

[0009] In another embodiment of the present invention, the electrochemical voltammetry is a linear voltammetry or a pulse voltammetry.

[0010] In another embodiment of the invention, the potential range is between 0 millivolts and 600 millivolts.

[0011] In another embodiment of the invention, the potential range is between 300 millivolts and 450 millivolts.

[0012] In another embodiment of the present invention, during the detection step, the ambient temperature is between 20 degrees Celsius and 35 degrees Celsius.

[0013] In another embodiment of the present invention, when the sugar in the sugar-containing test sample is a disaccharide or a polysaccharide, a catalytic step is performed before the detection step, wherein a catalytic voltage is applied to the test solution to catalyze the hydrolysis reaction of the disaccharide or the polysaccharide.

[0014] In another embodiment of the present invention, when the operating temperature of the detection step is higher or lower than the ambient temperature, a temperature correction step is performed, and the current value data is corrected by the potential temperature correction data corresponding to the operating temperature to obtain the calibration current value data.

[0015] In another embodiment of the present invention, in the analysis step, sugar concentration data is obtained by comparing sugar potential standard data and current value data in a standard database. The method for establishing the standard database includes the following steps: Solution preparation step: multiple sugar-containing samples with different sugar concentrations are mixed with a copper sulfate solvent to form multiple test solutions, and the known sugar concentration data of each sugar-containing sample is recorded; Current detection step: at room temperature without additional heating, each test solution is dropped onto an electrode test piece, and a driving voltage is applied to the electrode test piece by electrochemical voltammetry to activate the redox reaction of each test solution, and the current value of the electrode test piece is monitored in the potential range to obtain the corresponding current value data for each sugar-containing sample; Database establishment step: the known sugar concentration data and the corresponding current value data are integrated into sugar potential standard data, and a standard database storing the sugar potential standard data is established accordingly.

[0016] Therefore, the present invention can perform the detection steps while maintaining room temperature without the need for a heating process, thereby effectively solving the production safety problems caused by the old heating process.

[0017] Furthermore, the present invention uses the electrochemical voltammetry method to detect sugar concentration, enabling the present invention to measure the sugar concentration in the sugar-containing test sample in a very short time. Thus, the present invention has the advantages of simple operation, high detection efficiency, and high detection accuracy. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0019] Figure 1 This is a schematic flowchart of the electrochemical method for detecting sugar concentration according to an embodiment of the present invention.

[0020] Figure 2 This is a schematic flowchart of a method for electrochemical detection of sugar concentration according to another embodiment of the present invention.

[0021] Figure 3 This is a schematic diagram illustrating an embodiment of the present invention, used to show the injection of the test solution into the electrode sample.

[0022] Figure 4 This is a schematic diagram of the current value curve according to an embodiment of the present invention.

[0023] Figure 5 This is a schematic diagram of linear comparison of current concentration according to an embodiment of the present invention.

[0024] Figure 6 This is a schematic diagram showing a linear comparison of temperature and correction coefficient according to an embodiment of the present invention.

[0025] Figure 7 This is a flowchart illustrating the steps of a method for establishing a standard database according to an embodiment of the present invention.

[0026] The correspondence between the reference numerals and component names is as follows: 100. Method for electrochemical detection of sugar concentration; 200. Method for establishing a standard database; 10. Detection device; 20. Electrode test piece; 30. Test solution; S1. Test solution preparation steps; S2. Detection steps; S3. Analytical steps; S4. Catalytic steps; S5. Temperature correction steps; P1. Solution preparation steps; P2. Current detection steps; P3. Database establishment steps. Detailed Implementation

[0027] To facilitate the explanation of the central idea of ​​this invention, specific embodiments are described below. The various objects in the embodiments are depicted at a scale suitable for illustrative purposes, rather than being drawn to the scale of actual components; this is stated beforehand. The technical solution of this invention is described below with reference to the accompanying drawings and specific embodiments.

[0028] Please see Figures 1 to 7The diagram illustrates an electrochemical method 100 for detecting sugar concentration according to an embodiment of the present invention, comprising a test solution preparation step S1, a detection step S2, and an analysis step S3. The present invention can detect sugar concentration in sugary liquids, particularly sugar concentrations at low levels, such as sugar concentrations below 5%. The sugars in the sugary liquids can be monosaccharides such as glucose and fructose; disaccharides such as lactose, maltose, and sucrose; and polysaccharides such as starches, all of which are detectable by the present invention.

[0029] Please see Figure 1 and Figure 3 As shown, the electrochemical detection of the present invention measures the sugar concentration of a test solution 30 containing sugar using a detection device 10 and an electrode test piece 20. The test solution 30 is obtained through the test solution preparation step S1.

[0030] Test solution preparation step S1: Mix the sugar-containing test sample with a copper sulfate-containing solvent to form test solution 30. The volume of the sugar-containing test sample is 0.1 mL to 1 mL, thereby saving the cost of preparing the mixed solution through micro-detection of small volumes; the copper sulfate concentration of the copper sulfate-containing solvent is between 0.01 M and 1.5 M.

[0031] Detection step S2: In a room temperature environment without additional heating, the test solution 30 is dropped into the electrode test piece 20, and a driving voltage is applied to the electrode test piece 20 by electrochemical voltammetry to activate the redox reaction of the test solution 30. The current value of the electrode test piece 20 is monitored in a potential range to obtain current data (e.g., ...). Figure 4 (As shown).

[0032] In this embodiment of the invention, the detection time of the electrochemical voltammetry is less than 1 minute. Therefore, by means of the present invention, the sugar concentration in the sugar-containing test sample can be measured in a very short time, thereby improving the detection efficiency. The electrochemical voltammetry is a linear voltammetry or a pulse voltammetry.

[0033] like Figure 4 As shown, in this embodiment of the invention, the potential range is between 0 mV and 600 mV, preferably between 300 mV and 450 mV, so as to obtain more accurate current value data and eliminate current value data with excessive error.

[0034] In this embodiment of the invention, the temperature of the room temperature environment is between 20 degrees Celsius and 35 degrees Celsius, more preferably between 20 degrees Celsius and 25 degrees Celsius. Thus, the present invention abandons the old method of activating the redox reaction by heating and instead activates the redox reaction of the test solution 30 by electrochemical voltammetry. This allows the operating environment of the detection step S2 to be controlled at room temperature (i.e., 20 degrees Celsius to 35 degrees Celsius), effectively solving the production safety problems and complicated detection steps caused by the old method of activating by heating.

[0035] Analysis step S3: Analyze the current value data to obtain the sugar concentration data of the sugar-containing sample. In this embodiment of the invention, the sugar concentration data is obtained by comparing the sugar potential standard data in a standard database with the current value data. For example, please refer to... Figure 4 and Figure 5 As shown, the present invention can utilize the sugar potential standard data (i.e. Figure 5 The linear comparison graph of current and sugar concentration shown is used to obtain the sugar concentration data corresponding to the current value data (that is, when the potential range is 375 mV and the current value data is 170 μA, the sugar concentration data is 1%).

[0036] Please see Figure 2 As shown, when the sugar in the sugar-containing test sample is a disaccharide (e.g., sucrose) or a polysaccharide (e.g., starch), a catalytic step S4 can be performed before the detection step S2. A catalytic voltage is applied to the test solution 30 to catalyze the hydrolysis reaction of the disaccharide or the polysaccharide, causing the disaccharide or the polysaccharide to hydrolyze into monosaccharides. This allows the present invention to determine the concentration of the disaccharide or the polysaccharide in the sugar-containing test sample by detecting the concentration of monosaccharides.

[0037] In this embodiment of the invention, the catalytic voltage is a scanning voltage of -1000 mV to 1000 mV, and the scanning speed of the catalytic voltage is between 20 mV / s and 250 mV / s. Preferably, the scanning speed of the catalytic voltage is 100 mV / s. It should be noted that if the scanning speed of the catalytic voltage is lower than 20 mV / s, the catalytic effect of the catalytic voltage will be greatly reduced, causing the disaccharide or the polysaccharide to fail to hydrolyze normally, resulting in inaccurate sugar concentration data obtained from the final analysis; if the scanning speed of the catalytic voltage is higher than 250 mV / s, the electrode sample 20 is prone to structural damage due to exceeding its load-bearing capacity. Please see Figure 2As shown, when the operating temperature is detected by the temperature detection device to be higher or lower than the current room temperature set by the standard database in detection step S2, a temperature correction step S5 is first performed in detection step S2. The current value data is corrected by the potential temperature correction data corresponding to the operating temperature to obtain the calibration current value data.

[0038] The current value data is obtained by multiplying the potential-temperature correction data by a correction coefficient corresponding to the operating temperature. Furthermore, if the operating temperature is higher than the ambient temperature, the correction coefficient is less than 1 and greater than 0; if the operating temperature is lower than the ambient temperature, the correction coefficient is greater than 1. Therefore, by correcting the current value data using the correction coefficient, this invention can improve the detection accuracy of the sugar concentration data. Please refer to [link to relevant documentation]. Figure 6 As shown, for example, when the ambient temperature is set to 25 degrees Celsius, if the operating temperature (for example, 30 degrees Celsius) is higher than the ambient temperature (25 degrees Celsius), the correction factor is 0.8; if the operating temperature (for example, 10 degrees Celsius) is lower than the ambient temperature (25 degrees Celsius), the correction factor is 1.6.

[0039] Additionally, it should be noted that the temperature calibration step S5 is performed when the operating temperature is within a temperature range (in this invention, the temperature range is set to be between 10 degrees Celsius and 45 degrees Celsius). If the temperature detection device detects that the operating temperature exceeds the temperature range, it will identify the operating temperature as an abnormal temperature and issue a warning to inform the user that the current environment is not suitable for testing.

[0040] Please see Figure 7 As shown, the present invention further illustrates a method 200 for establishing the standard database, including a solution preparation step P1, a current detection step P2, and a database establishment step P3.

[0041] Solution preparation step P1: Mix multiple sugar-containing samples with different sugar concentrations with the copper sulfate solvent to form multiple test solutions, and record the known sugar concentration data of each sugar-containing sample.

[0042] Current detection step P2: In the room temperature environment, without additional heating, each of the test solutions is dropped onto the electrode test piece 20, and the driving voltage is applied to the electrode test piece 20 by electrochemical voltammetry to activate the redox reaction of each of the test solutions. The current value of the electrode test piece 20 is monitored in the potential range to obtain the corresponding current value data for each of the sugar-containing samples. The corresponding current value data includes multiple current value curves, and each current value curve corresponds to a known sugar concentration.

[0043] Database establishment step P3: Integrate the known sugar concentration data and the corresponding current value data into the sugar potential standard data, and establish a standard database storing the sugar potential standard data. Please refer to [link / reference]. Figure 5 As shown, the linearity between the current and sugar concentration in the sugar potential standard data established by this invention can reach as high as 0.9905, indicating that the sugar concentration detection achieved using this invention has a considerably high accuracy. Furthermore, users can store the standard database on a terminal device or cloud server, allowing for convenient storage and maintenance of the standard database.

[0044] In addition, the potential-temperature correction data of the present invention can also be stored in the standard database, so that users can maintain and update the sugar potential standard data and the potential-temperature correction data together through the standard database.

[0045] Therefore, the present invention has the following advantages: 1. The present invention can perform the detection step S2 while maintaining room temperature without the need for a heating process, thereby effectively solving the production safety problems caused by the old heating process.

[0046] 2. The present invention uses the electrochemical voltammetry method to detect sugar concentration, enabling the present invention to measure the sugar concentration in the sugar-containing test sample in a very short time. Therefore, the present invention has the advantages of simple operation, high detection efficiency and high detection accuracy.

[0047] 3. The present invention sets the potential range to 0 mV to 600 mV (preferably, the potential range is between 300 mV and 450 mV), thereby obtaining more accurate current value data and eliminating current value data with excessive errors.

[0048] 4. The present invention accelerates the catalysis of the test solution 30 through the catalytic step S4, and makes the present invention applicable not only to monosaccharides, but also to the detection of disaccharides and polysaccharides.

[0049] 5. The present invention can correct the current value data through the correction coefficient, thereby maintaining the detection accuracy of the sugar concentration data even when the operating temperature changes.

[0050] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. In addition, those skilled in the art can combine and integrate the different embodiments or examples described in this specification.

[0051] Furthermore, the technical solutions of the various embodiments can be combined with each other, but only if they are feasible for those skilled in the art. If the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0052] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A method for electrochemical detection of sugar concentration, characterized in that, include: Preparation steps of the test solution: Mix the sugar-containing test sample with a copper sulfate-containing solvent to form the test solution; Detection steps: In a room temperature environment, without the need for additional heating, the test solution is dropped onto the electrode test piece, and a driving voltage is applied to the electrode test piece by electrochemical voltammetry to activate the redox reaction of the test solution. The current value of the electrode test piece is monitored in a potential range to obtain current value data. as well as Analysis steps: Analyze the current value data to obtain the sugar concentration data of the sugar-containing test sample.

2. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, The volume of the sugar-containing test sample is 0.1 ml to 1 ml.

3. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, The detection time of the electrochemical voltammetry is less than 1 minute.

4. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, The electrochemical voltammetry method is either linear voltammetry or pulse voltammetry.

5. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, The potential range is between 0 millivolts and 600 millivolts.

6. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, The potential range is between 300 millivolts and 450 millivolts.

7. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, In the detection step, the temperature of the ambient temperature is between 20 degrees Celsius and 35 degrees Celsius.

8. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, When the sugar in the sugar-containing test sample is a disaccharide or a polysaccharide, a catalytic step is performed before the detection step, in which a catalytic voltage is applied to the test solution to catalyze the hydrolysis reaction of the disaccharide or the polysaccharide.

9. The method for electrochemical detection of sugar concentration as described in claim 1, characterized in that, In the analysis step, the sugar concentration data is obtained by comparing the sugar potential standard data and the current value data in the standard database. The method for establishing the standard database includes the following steps: Solution preparation steps: Mix multiple sugar-containing samples with different sugar concentrations with the copper sulfate-containing solvent to form multiple test solutions, and record the known sugar concentration data of each sugar-containing sample; Current detection steps: In the room temperature environment, without additional heating, each test solution is dropped onto the electrode plate, and the driving voltage is applied to the electrode plate by electrochemical voltammetry to activate the redox reaction of each test solution. The current value of the electrode plate is monitored in the potential range to obtain the corresponding current value data for each sugar-containing sample; and Database establishment steps: Integrate the known sugar concentration data and the corresponding current value data into the sugar potential standard data, and establish the standard database storing the sugar potential standard data.

10. The method for electrochemical detection of sugar concentration as described in claim 9, characterized in that, When the operating temperature of the detection step is higher or lower than the current room temperature set in the standard database, a temperature correction step is performed. The current value data is corrected by the potential temperature correction data corresponding to the operating temperature to obtain the calibration current value data.

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