Citrus colouring sweetening composition and use

Abstract

The application belongs to the field of citrus cultivation, and particularly relates to a citrus coloring and sweetening composition and application. The main components of the citrus coloring and sweetening composition are maleic hydrazide and ethylene, and the mass ratio of the maleic hydrazide to the ethylene is 2:(1-4). The application is prepared by compounding maleic hydrazide and ethylene. The test results show that the application can promote early coloring of citrus, prevent reverse acidification of citrus after application of maleic hydrazide, increase average sugar content of citrus, has no negative effect on growth of citrus, has no pollution to the environment, and can be widely used in planting and production of different regions and different citrus varieties.

Description

Technical Field

[0001] This invention belongs to the field of citrus cultivation, specifically relating to a citrus coloring and sweetening composition and its application. Background Technology

[0002] Citrus fruits, belonging to the Rutaceae family and the Citrus genus, are labor- and technology-intensive agricultural products with significant market competitive advantages, substantial domestic and international demand growth, and considerable development potential. Citrus is the world's largest fruit category and third-largest traded agricultural product, holding a very important position in international agricultural trade. Globally, over 140 countries and regions produce citrus fruits, primarily distributed in warm, humid regions south of north latitude, with Guangxi Zhuang Autonomous Region being my country's largest production area. Citrus fruits are highly nutritious and possess significant medicinal and health benefits, making them popular in both domestic and international fruit markets.

[0003] In recent years, China's citrus fruit industry has developed rapidly, with continuous increases in cultivation area, production volume, and per capita consumption. Some industrialized production bases for citrus fruits have been established, and there is a good foundation for accelerated development. Export volume has also increased significantly. However, there are also problems such as variety degradation, quality decline, and unreasonable varieties and ripening periods. Therefore, improving the quality of citrus fruits plays a crucial role in the future development of citrus.

[0004] Research data shows that indole-3-ethyl can promote citrus coloring, increase soluble solids, and improve fruit quality. Experimental results indicate that spraying citrus trees with indole-3-ethyl can advance coloring by about seven days, but it can also cause acid reflux in the fruit. How to ensure early coloring while preventing acid reflux has become a key research focus. Summary of the Invention

[0005] The purpose of this invention is to provide a citrus coloring and sweetening composition that solves the problem of acid reflux caused by using indole-3-ethyl to promote citrus coloring.

[0006] The second objective of this invention is to provide the application of the above-mentioned citrus coloring and sweetening composition in citrus cultivation, thereby solving the problem of how to promote citrus coloring while preventing citrus acid reversion.

[0007] To achieve the first objective mentioned above, the technical solution adopted by this invention is as follows:

[0008] A citrus coloring and sweetening composition, the main components of which are indole-3-methyl ester and methylamine ester, wherein the mass ratio of indole-3-methyl ester to methylamine ester is 2:(1-4).

[0009] This invention is a pioneering invention. By combining indole and methyl methacrylate, experimental results show that it can promote early coloring of citrus, prevent acid reversion in citrus after the application of indole alone, and increase the average sugar content of citrus. It has no negative impact on citrus growth and no pollution to the environment. It can be widely used in the planting and production of different regions and different citrus varieties.

[0010] Preferably, the mass ratio of the indole-methyl ester to the methyl ester is 2:(1-2).

[0011] To achieve the second objective mentioned above, the technical solution adopted by the present invention is as follows:

[0012] The above-mentioned citrus coloring and sweetening composition is applied to citrus cultivation by spraying the citrus trees with the citrus coloring and sweetening composition to promote citrus coloring, prevent acid reflux, and increase sugar content.

[0013] Spraying citrus trees with the above-mentioned citrus coloring and sweetening composition does not affect the coloring of citrus, can effectively prevent citrus from acidifying, and can increase the sugar content of citrus, significantly improving the quality of citrus and enhancing its competitiveness in the market.

[0014] Preferably, the spraying concentration of indole and terbinyl ester in the citrus coloring and sweetening composition is 50-200 mg / L.

[0015] More preferably, the spraying concentration of indole ester in the citrus coloring and sweetening composition is 100 mg / L, and the spraying concentration of amine ester is 50-100 mg / L.

[0016] Preferably, the spraying time is during the early stage of coloring.

[0017] Preferably, the citrus fruits include Murcott mandarins and Wogan mandarins. Attached Figure Description

[0018] Figure 1 These are colorized images of the Murcott oranges before and 7 days after medication in Example 4 of this invention;

[0019] Figure 2 These are colorized images of Murcott oranges after 14 days and 28 days of medication, as described in Example 4 of this invention.

[0020] Figure 3 These are colorized images of the Wogan oranges before and 7 days after medication in Example 5 of this invention;

[0021] Figure 4 Colored images of Wogan oranges treated with the drug for 14 days and 28 days in Example 5 of this invention. Detailed Implementation

[0022] The octanoic acid ester is diethylaminoethanol octanoate malate, which can be prepared according to the method described in Chinese invention patent No. CN 113754550 B, which was authorized on March 22, 2024.

[0023] This invention demonstrates through treatment experiments with single agent of indole-3-methyl ester, single agent of indole-3-methyl ester, and a combination of indole-3-methyl ester and indole-3-methyl ester that indole-3-methyl ester and indole-3-methyl ester are a group of highly efficient agricultural compositions suitable for citrus cultivation. They can enhance the coloring effect of indole-3-methyl ester, prevent acid reflux when using single agent of indole-3-methyl ester, and increase the sugar content of citrus products.

[0024] The following will provide a clear and complete description of the concept and technical effects of the present invention in conjunction with embodiments, so as to fully understand the purpose, features and effects of the present invention. Unless otherwise specified, the methods used in the embodiments are conventional methods; unless otherwise specified, the materials and reagents used are commercially available. In the following embodiments, unless otherwise specified, "%" refers to mass fraction.

[0025] I. Specific Embodiments of the Citrus Coloring and Sweetening Composition of the Present Invention

[0026] Example 1

[0027] The citrus coloring and sweetening composition of this embodiment consists of indole-methyl ester and methylamine ester, with a mass ratio of indole-methyl ester to methylamine ester of 2:1.

[0028] Example 2

[0029] The citrus coloring and sweetening composition of this embodiment consists of indole-methyl ester and methylamine ester, with a mass ratio of indole-methyl ester to methylamine ester of 1:1.

[0030] Example 3

[0031] The citrus coloring and sweetening composition of this embodiment consists of indole-methyl ester and methylamine ester, with a mass ratio of indole-methyl ester to methylamine ester of 1:2.

[0032] When using the citrus coloring and sweetening compositions of the above embodiments, indole and terbinafine can be applied separately to citrus trees, or they can be formulated into multifunctional preparations using conventional methods, achieving multiple functions such as promoting citrus coloring, preventing acid reflux, and increasing sweetness through a single application. Based on the above main components, conventional excipients (such as preservatives) can be further added to formulate agricultural preparations using conventional methods.

[0033] II. Specific Examples of the Application of Citrus Coloring and Sweetening Compositions in Citrus Cultivation

[0034] Example 4: Coloring and Sweetening Experiment of Chongzuo Maogugan Tangerines in Guangxi

[0035] The experiment was conducted during the early coloring stage of the Murcott mandarin orange. The early coloring stage refers to the period when the citrus fruit has just begun to turn yellow, with a coloring index generally ranging from 3% to 12%.

[0036] Preparation of indole-methyl ester solution: Dilute 15% indole-methyl ester emulsifiable concentrate with water to three concentrations of 50 mg / L, 100 mg / L and 200 mg / L respectively for use.

[0037] Preparation of methyl methacrylate solution: Dilute 15% methyl methacrylate soluble agent with water to a concentration of 200 mg / L before use.

[0038] Indole-methyl ester + methyl methacrylate compound treatment: Mix 15% indole-methyl ester emulsifiable concentrate and 15% methyl methacrylate soluble concentrate in a certain proportion, calculate the dilution ratio according to the required concentration, and use after dilution according to the dilution ratio.

[0039] Water was used as a blank control for the above treatments.

[0040] The entire experiment consists of the following steps:

[0041] Fruit setting: Select tangerine trees with growth conditions suitable for pesticide application, and select 5 clusters of fruit on the sunny side and 5 clusters on the shady side of the same tangerine tree. Take photos and label them. Randomly select 6 fruits from each tree to measure the initial sugar content data. Select 5 trees for each pesticide concentration as parallel treatment.

[0042] Application: Spray the diluted indole, methyl methacrylate, and their compound formulation evenly onto the leaves and fruit of citrus trees, applying 3L of solution to each tree.

[0043] Survey: The first survey was conducted 7 days after pesticide application. Photos were taken of each tree after fruit setting to assess the coloring index. Six fruits were randomly selected to measure sugar content and observe the sweetening effect. The second and third surveys were conducted at 14 and 28 days after pesticide application, with the same indicators as the first survey.

[0044] Sugar content: The soluble solids content in citrus fruits is measured using a handheld saccharimeter and displayed as a percentage.

[0045] Coloring index: The percentage of the fruit's surface area that is colored is expressed by an index or level (the percentage of the surface area of ​​the colored area of ​​each tree with labeled citrus fruits is calculated by averaging the percentage of the surface area of ​​each tree with 5 trees per treatment). It is an important indicator of the appearance quality of fruit and one of the standards for measuring fruit maturity.

[0046] The results of the investigation on the coloring index and sugar content of Murcott tangerines at different stages after pesticide application are shown in Tables 1 and 2, respectively. Images of the coloring of Murcott tangerines at different stages after pesticide application are shown below. Figure 1 , Figure 2 As shown.

[0047] Table 1. Survey of coloring index of Murcott oranges at different time points after pesticide application.

[0048]

[0049] Table 1 shows that the coloring index of Murcott oranges treated with indole-3-methyl ester was significantly higher than that of the control (CK), with a concentration of 100 mg / L showing the best effect. Treatment with indole-3-methyl ester combined with methyl methacrylate (MMA) had no adverse effect on the coloring of Murcott oranges and was slightly better than the single-agent treatment.

[0050] Table 2. Survey of sugar content in Murcott oranges at different time points after pesticide application.

[0051]

[0052] The experimental results are shown in Table 2. The sugar content of Murcott oranges treated with indole ester was significantly higher than that of the control group (CK) after 7 days of treatment. All three single-agent treatments were significantly better than the control group, with the 100 mg / L treatment showing the best effect. However, the sugar content after 14 days of treatment was lower than that after 7 days of treatment, indicating that spraying indole ester can increase the sugar content of Murcott oranges in the short term. Murcott oranges will experience acid reflux from 7 to 14 days after treatment. The acid reflux will improve when the 28-day survey is conducted, but the sugar content is not superior to that of the control group.

[0053] The sugar content of the blank control, the single agent of methyl methacrylate, and the combination of indole and methyl methacrylate in Murcott oranges steadily increased over time. Compared with the blank control, the single agent of methyl methacrylate also had a certain sweetening effect, but it was not significant.

[0054] According to the data in Table 2, the sugar content of the treatment with indole-3-methyl ester combined with methyl methacrylate steadily increased over time, which was significantly better than the CK blank control treatment and each single-dose treatment. Among them, the (100+100) mg / L compound treatment had the best effect, indicating that the indole-3-methyl ester combined with methyl methacrylate can not only solve the problem of acid reflux in Murcott oranges in the later stage of indole-3-methyl ester single-dose treatment, but also further increase the sugar content. Compared with the blank control, the sugar content was on average 1-2 percentage points higher.

[0055] Example 5: Coloring and Sweetening Experiment of Wogan Oranges in Wuming County, Nanning, Guangxi

[0056] The trial was conducted and the medication administration method was the same as in Example 5.

[0057] The results of the investigation on the coloring index and sugar content of Wogan mandarins at different stages after pesticide application are shown in Tables 3 and 4, respectively. Images of the coloring of Wogan mandarins at different stages after pesticide application are shown below. Figure 3 , Figure 4 As shown.

[0058] Table 3. Survey of coloring index of Wogan tangerines at different stages after pesticide application.

[0059]

[0060] Table 3 shows that the coloring index of Wogan oranges treated with indole monomethyl ester was significantly higher than that of the control (CK) treatment, with a concentration of 100 mg / L showing the best effect. Treatment with indole monomethyl ester combined with methyl methacrylate (MMA) had no adverse effect on the coloring of Wogan oranges.

[0061] Table 4. Survey of sugar content of Wogan oranges at different time points after pesticide application.

[0062]

[0063]

[0064] The experimental results are shown in Table 4. Seven days after treatment with indole-methyl, the sugar content of Wogan oranges treated with indole was on average about 1 percentage point higher than the control (CK). Higher concentrations showed better results, with the 100 mg / L treatment exhibiting the best effect. Fourteen days after treatment, the sugar content was lower than that after seven days, indicating that acid reflux occurred on Wogan oranges 7-14 days after indole application. At 28 days, the acid reflux improved, but the sugar content showed no advantage compared to the control.

[0065] The sugar content of Wogan oranges treated with blank control, single agent of methyl methacrylate, and compound treatment of indole-methyl methacrylate and methyl methacrylate gradually increased over time, and no acid reflux was observed.

[0066] According to the data in Table 4, the sugar content of the treatment with indole-3-methyl ester combined with methyl methacrylate steadily increased over time, which was significantly better than the CK blank control treatment and each single-agent treatment. Among them, the (100+100) mg / L compound treatment had the best effect, indicating that the indole-3-methyl ester combined with methyl methacrylate can solve the problem of citrus acid reflux that occurred in the later stage of indole-3-methyl ester single-agent treatment, and the sugar content was about 1 point higher on average than the blank control.

[0067] The above experiments show that the combination of indole-modified ester and methyl methacrylate can effectively prevent acid reversion in citrus fruits, increase their sugar content, significantly improve their quality, and enhance their market competitiveness. This compounding technology is safe to use and has no side effects. It can be widely applied to citrus cultivation in different regions and of different varieties, playing an important role in citrus production and having significant implications for agricultural production.

[0068] The present invention has been further described above with reference to the embodiments. Those skilled in the art should understand that, without departing from the concept of the present invention, various specific parameters in the above embodiments can be changed or modified to form multiple specific embodiments, all of which are common variations of the present invention, and will not be described one by one here.

Claims

1. A citrus coloring and sweetening composition, characterized in that, The main components are indole-3-methyl ester and methylamine ester, and the mass ratio of indole-3-methyl ester to methylamine ester is 2:(1-4).

2. The citrus coloring and sweetening composition according to claim 1, characterized in that, The mass ratio of the indole-modified ester to the ammonium ester is 2:(1-2).

3. The application of the citrus coloring and sweetening composition as described in claim 1 or 2 in citrus cultivation, characterized in that, Spraying the citrus coloring and sweetening composition onto citrus trees promotes citrus coloring, prevents acid reflux, and increases sugar content.

4. The application as described in claim 3, characterized in that, The spraying concentrations of indole and terbinyl ester in the citrus coloring and sweetening composition are both 50–200 mg / L.

5. The application as described in claim 4, characterized in that, The spraying concentration of indole ester in the citrus coloring and sweetening composition is 100 mg / L, and the spraying concentration of methyl methacrylate is 50-100 mg / L.

6. The application as described in claim 3, 4, or 5, characterized in that, The application time is during the early coloring stage.

7. The application as described in claim 6, characterized in that, The citrus fruits include Murcott mandarins and Wogan mandarins.

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