Cutting seedling rooting agent and preparation method thereof

Abstract

The invention discloses a cutting seedling rooting agent and a preparation method thereof, and relates to the technical field of garden horticulture, and the cutting seedling rooting agent comprises the following raw materials by weight: 5-15% of a citrus pectin oligosaccharide-spermidine compound, 0.0001-0.0005% of a brassinolide nanoemulsion, 2-5% of proline, 3-8% of sugar alcohol calcium, 5-10% of modified attapulgite, 0.25-0.55% of a surfactant, and the balance of deionized water. After use, the rooting effect is good and the stability is high.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of gardening, in particular to a cutting seedling rooting agent and a preparation method thereof. BACKGROUND

[0002] Cutting is a traditional plant asexual propagation technology, which is initially used as a propagation method of easy-rooting plants. After years of development, cutting technology is becoming mature and is applied in a wider range. Many flowers, trees, fruit trees and economic trees are mainly propagated by cutting. In order to improve the survival rate of cutting, people have made a lot of attempts to promote plant cutting propagation rooting.

[0003] The existing rooting agents mainly rely on single or composite chemical synthetic plant growth regulators (such as IBA, NAA), which have problems of unstable effect, poor effect on difficult-rooting plants, easy drug damage and environmental residue. Although biological stimulants (such as seaweed extract) are safe, the active components for promoting rooting are unclear and the effect is slow. SUMMARY

[0004] The first technical problem to be solved by the present application is to provide a cutting seedling rooting agent with good effect and high stability in view of the deficiencies in the prior art.

[0005] To solve the above-mentioned first technical problem, the technical solution of the present application is:

[0006] A cutting seedling rooting agent, which is composed of the following raw materials in percentage by weight:

[0007] Citrus pectic oligosaccharide- spermidine complex: 5-15%

[0008] Nanolipid of brassinolide: 0.0001-0.0005%

[0009] Proline: 2-5%

[0010] Calcium sugar alcohol: 3-8%

[0011] Modified attapulgite: 5-10%

[0012] Surfactant: 0.25-0.55%

[0013] Deionized water: the balance.

[0014] Preferably, the preparation method of the citrus pectic oligosaccharide-spermidine complex comprises the following steps:

[0015] A. Extracting pectin from citrus peel and preparing citrus pectic oligosaccharide with an average degree of polymerization of 3-7 by hydrogen peroxide-ascorbic acid method degradation;

[0016] B. Mix pecticoligosaccharide and spermidine in a mass ratio of 5:1 in a phosphate buffer solution at pH 7.5, add sodium cyanoborohydride as a reducing agent, and react at 55-60°C for 5-6h;

[0017] C. Purify the reaction solution through an ultrafiltration membrane with a molecular weight cutoff of 1000 Da to remove unreacted spermidine and small molecular impurities, collect the filtrate, and freeze-dry to obtain a white powder of the complex.

[0018] Preferably, the preparation method of the brassinolide nanoemulsion comprises the following steps:

[0019] S1. Dissolve brassinolide in soybean oil as an oil phase;

[0020] S2. Dissolve phospholipid and Tween-80 in deionized water as an aqueous phase;

[0021] S3. Slowly add the oil phase of S1 to the aqueous phase of S2 under moderate stirring at 500-800 rpm, and after the addition is completed, use a high-speed shearing machine to shear at a speed of 8,000-12,000 rpm for 5-10 min to form a coarse emulsion;

[0022] S4. Pass the coarse emulsion through a high-pressure homogenizer at a pressure of 10,000-20,000 psi for 3-5 cycles to obtain a brassinolide nanoemulsion;

[0023] wherein the content of brassinolide in the brassinolide nanoemulsion is 0.5-1.5% w / w, the content of soybean oil is 7.5-13.5% w / w, the content of phospholipid is 8-15% w / w, and the content of Tween-80 is 10-20% w / w.

[0024] Preferably, the preparation method of the modified attapulgite comprises the following steps:

[0025] a. Crush and grind the natural attapulgite ore, pass it through a 200-mesh sieve, and obtain attapulgite powder;

[0026] Mix 4 mol / L hydrochloric acid and 2 mol / L citric acid in a volume ratio of 3:1, stir until uniform, and obtain a mixed acid solution;

[0027] Mix the attapulgite powder and the mixed acid solution in a solid-liquid ratio of 1:10, place them in a reactor equipped with a condensation reflux device, and stir at a constant temperature of 85°C for 4h;

[0028] After the reaction is completed, centrifugal separation is performed, the filter cake is repeatedly washed with deionized water until the supernatant is neutral, and the washed material is air-dried at 105°C to a constant weight to obtain acid-activated attapulgite;

[0029] b. The acid-activated palygorskite is laid flat in a crucible and placed in a muffle furnace, and heated at a rate of 3℃ / min from room temperature to 380℃, and kept at this temperature for 2h;

[0030] After the heat treatment is completed, the palygorskite is removed from the furnace after naturally cooling to below 150℃, and immediately transferred to a desiccator to cool to room temperature, to obtain the heat-activated palygorskite;

[0031] c. The gamma-aminopropyltriethoxysilane is dissolved in 90% aqueous ethanol, and hydrolyzed by stirring for 30min, to prepare a silane hydrolyzate with a concentration of 2% v / v;

[0032] The heat-activated palygorskite is added to the silane hydrolyzate at a solid-liquid ratio of 1:8, and reacted at 60℃ and 80-120rpm for 5-6h;

[0033] After the reaction is completed, the solid product is collected by centrifugation, washed with anhydrous ethanol three times to remove the physically adsorbed silane, and finally dried at 80℃ under vacuum for 6h, to obtain the modified palygorskite.

[0034] Preferably, the surfactant is a mixture of tea saponin, alkyl polyglycoside and phosphatidylcholine, wherein the tea saponin accounts for 0.1-0.3% of the total weight of the rooting agent, the alkyl polyglycoside accounts for 0.1-0.15% of the total weight of the rooting agent, and the phosphatidylcholine accounts for 0.05-0.1% of the total weight of the rooting agent.

[0035] The second technical problem to be solved by the present application is to provide a preparation method of a cutting seedling rooting agent, which has good effect and high stability.

[0036] To solve the above-mentioned second technical problem, the technical scheme of the present application is:

[0037] A preparation method of a cutting seedling rooting agent, comprising the following steps:

[0038] I. Deionized water accounting for 40-60% of the total formulation amount is added to the reactor, and then proline, calcium sugar alcohol, and citrus pectin oligosaccharide- spermidine complex are sequentially added and fully stirred and dissolved;

[0039] II. Under high-speed stirring, modified palygorskite is first added, and after being dispersed by stirring, brassinolide nanoemulsion is added;

[0040] III. The surfactant is pre-stirred and mixed, dissolved in warm water, and then added to the above-mentioned reactor;

[0041] IV. The remaining amount of deionized water is added, and the stirring is continued until a uniform and stable suspension is formed, to obtain the cutting seedling rooting agent.

[0042] Preferably, in step I, the stirring speed is set to 80-100 rpm when deionized water is added, and after all raw materials are added, the stirring speed is increased to 180-200 rpm and the stirring is continued for 40-60 min, and the dissolution temperature is 25-35 DEG C.

[0043] Preferably, in step II, the stirring speed is adjusted to 350-400 rpm before feeding, and after the modified attapulgite is added and stirred for 20-30 min, the brassinolide nanoemulsion is continuously added, and after all the feeding is completed, the stirring speed is adjusted to 550-600 rpm and the stirring is continued for 20-40 min, and the temperature of the material liquid is controlled to be not more than 40 DEG C by water bath or jacket cooling during the feeding process.

[0044] Preferably, in step III, the mass ratio of the surfactant to warm water is 2.5-5.5:10, the temperature of the warm water is 40-45 DEG C, and the stirring speed is 150-200 rpm.

[0045] Preferably, in step IV, the stirring speed is 120-150 rpm, and the stirring time is 30-40 min.

[0046] Due to the adoption of the above technical solutions, the application has the following beneficial effects:

[0047] 1. Citrus pectin oligosaccharide, as a plant elicitor, can simulate pathogen invasion, activate plant defense response and systemic resistance, mobilize resources, and indirectly promote wound healing and root source differentiation. Spermidine is a core member of polyamines and directly participates in cell division, differentiation and stress response. The present application covalently combines the two by reductive amination, realizes the precise coupling and synergistic amplification of defense signals and growth signals, and when acting on the cut of the cutting seedling, it can simultaneously and efficiently start two key physiological pathways, significantly up-regulate the expression of genes related to rooting (such as auxin response factor ARF, nitrile hydrolase NIT, etc.), and powerfully induce the initiation and development of root source, which is much better than the simple physical mixing of the two, and solves the problems of limited effect and instability of a single signal molecule.

[0048] 2. Brassinolide is a highly efficient hormone, but it is difficult to dissolve in water and is easy to photodegrade. The present application prepares it into nanoemulsion, and soybean oil protects the activity of brassinolide as the oil phase; the complex emulsification system of phospholipid and Tween-80 forms a nano-sized and highly stable emulsion droplet. This not only solves the problems of water solubility and stability of brassinolide, but more importantly, the nano-sized emulsion droplet is easier to adhere, penetrate and conduct through the phloem at the cutting seedling cut, so that brassinolide can be precisely delivered to the action site, and it can play a strong effect of promoting cell division and elongation at a very low concentration (0.0001-0.0005%).

[0049] 3. The present application carries out three-step fine modification of "acid activation-thermal activation-silanization" on attapulgite. Acid activation and thermal activation greatly improve its specific surface area and pore volume, forming strong adsorption capacity. The subsequent modification of γ-aminopropyl triethoxysilane is a key creative step, which introduces organic amino functional groups on the surface of the carrier. These amino groups can specifically bind to active ingredients such as pectic oligosaccharides and proline through hydrogen bonds, ionic bonds and other forces. The modified attapulgite can adsorb and fix most water-soluble active substances, and then slowly release them in aqueous solution, avoiding the rapid loss of active ingredients. At the same time, when nanoemulsion is added, the oily droplets can be physically adsorbed by the hydrophobic region of attapulgite, but will not be completely "locked". This design allows both water-soluble and fat-soluble active ingredients to achieve long-term and stable release, and once used, the effect is sustained, ensuring that the cuttings maintain an optimal hormone and nutrient environment throughout the critical rooting period.

[0050] 4. Proline, as an efficient osmotic adjustment substance, can rapidly accumulate to maintain cell osmotic pressure, protect enzyme activity and membrane structural integrity under in vitro water stress of cuttings. At the same time, it is also an active oxygen scavenger that can alleviate oxidative stress at the cut surface, creating a stable internal environment for root development. Sugar alcohol chelated calcium has excellent mobility and absorption efficiency, and can quickly provide sufficient calcium source for newly formed root cells, enhancing cell wall strength and promoting root development. It plays a synergistic role with proline in maintaining cell membrane stability and resisting environmental stress.

[0051] 5. The surfactant system of the present application is not a single component, but a scientific combination of tea saponin, alkyl polyglycoside and phosphatidylcholine, which has both functionality and environmental friendliness. This complex system can form an efficient delivery network by improving spreadability with tea saponin, promoting penetration with alkyl polyglycoside, and assisting transmembrane with phosphatidylcholine, ensuring that all beneficial ingredients are fully absorbed and utilized. At the same time, this complex system can also act as a dispersant to effectively prevent the sedimentation of solid particles such as modified attapulgite, ensuring the storage stability of the product. DETAILED DESCRIPTION

[0052] The present application will be further described in conjunction with the following examples. Example 1

[0053] A cutting root agent consisting of the following weight percentage of raw materials:

[0054] Citrus pectic oligosaccharide-epinephrine complex: 5%

[0055] Nanometer emulsion of brassinolide: 0.0001%

[0056] Proline: 2%

[0057] Calcium sugar alcohol: 3%

[0058] Modified palygorskite: 5%

[0059] Surfactant: 0.25% (of which tea saponin 0.1%, alkyl polyglycoside 0.1%, phosphatidylcholine 0.05%)

[0060] Deionized water: 84.7499% Example 2

[0061] A cutting seedling rooting agent, consisting of the following raw materials in percentage by weight:

[0062] Citrus pectic oligosaccharide-spermidine complex: 10%

[0063] Brassinolide nanoemulsion: 0.0003%

[0064] Proline: 3.5%

[0065] Calcium sugar alcohol: 5.5%

[0066] Modified palygorskite: 7.5%

[0067] Surfactant: 0.4% (of which tea saponin 0.2%, alkyl polyglycoside 0.125%, phosphatidylcholine 0.075%)

[0068] Deionized water: 73.0997% Example 3

[0069] A cutting seedling rooting agent, consisting of the following raw materials in percentage by weight:

[0070] Citrus pectic oligosaccharide-spermidine complex: 15%

[0071] Brassinolide nanoemulsion: 0.0005%

[0072] Proline: 5%

[0073] Calcium sugar alcohol: 8%

[0074] Modified palygorskite: 10%

[0075] Surfactant: 0.55% (of which tea saponin 0.3%, alkyl polyglycoside 0.15%, phosphatidylcholine 0.1%)

[0076] Deionized water: 61.4495% Example 4

[0077] The preparation method of the cutting seedling rooting agent in Examples 1-3, comprising the following steps:

[0078] I. Add 50% of the total amount of deionized water into the reactor, set the stirring speed to 90 rpm, then add proline, calcium sugar alcohol, and citrus pectin oligosaccharide- spermidine complex in turn, increase the stirring speed to 190 rpm, and stir at 30℃ for 50 min to fully dissolve;

[0079] II. Adjust the stirring speed to 380 rpm, then add modified attapulgite and stir for 25 min, and then add brassinolide nanoemulsion, adjust the stirring speed to 600 rpm and continue stirring for 30 min. The temperature of the material liquid should be controlled to not exceed 40℃ during the feeding process;

[0080] III. Pre-mix the surfactant at 180 rpm, dissolve it in warm water, and then add it to the above reactor. The mass ratio of surfactant to warm water is 4:10, and the temperature of the warm water is 42℃;

[0081] IV. Add the remaining amount of deionized water and continue stirring at 130 rpm for 35 min until a uniform and stable suspension is formed, and the cutting seedling rooting agent is obtained.

[0082] The preparation method of the citrus pectin oligosaccharide-spermidine complex includes the following steps:

[0083] A. Extract pectin from citrus peel and prepare citrus pectin oligosaccharide with an average degree of polymerization of 3-7 by hydrogen peroxide-ascorbic acid method degradation;

[0084] B. Mix the pectin oligosaccharide and spermidine in a mass ratio of 5:1 in a phosphate buffer solution at pH 7.5, add sodium cyanoborohydride as a reducing agent, and react at 58℃ for 5.5h;

[0085] C. Purify the reaction solution through an ultrafiltration membrane with a molecular weight cutoff of 1000 Da to remove unreacted spermidine and small molecular impurities, collect the filtrate, and freeze-dry to obtain a white powder of the complex.

[0086] The preparation method of the brassinolide nanoemulsion includes the following steps:

[0087] S1. Dissolve brassinolide in soybean oil as the oil phase;

[0088] S2. Dissolve phospholipid and Tween-80 in deionized water as the water phase;

[0089] S3. Under moderate stirring at 600 rpm, slowly add the oil phase of S1 to the water phase of S2, and after the addition is complete, use a high-speed shearing machine to shear at a speed of 10,000 rpm for 5-10 min to form a coarse emulsion;

[0090] S4. The crude emulsion was circulated through a high-pressure homogenizer at a pressure of 15,000 psi for 3-5 times to obtain the brassinolide nanoemulsion;

[0091] In the brassinolide nanoemulsion, the content of brassinolide is 1.0% w / w, the content of soybean oil is 10% w / w, the content of phospholipid is 12.5% w / w, the content of Tween-80 is 15% w / w, and the content of deionized water is 61.5% w / w.

[0092] The preparation method of the modified attapulgite includes the following steps:

[0093] a. The natural attapulgite ore was crushed, ground, and sieved through a 200-mesh screen to obtain attapulgite powder;

[0094] A mixed acid solution was obtained by mixing 4 mol / L hydrochloric acid and 2 mol / L citric acid at a volume ratio of 3:1 and stirring uniformly; the hydrochloric acid is responsible for strongly dissolving carbonate and other impurities, while the citric acid, as an organic weak acid, can more gently and selectively remove metal oxide impurities such as iron and aluminum through complexation, avoiding excessive damage to the pore structure, and its residues can preliminarily modify the surface.

[0095] The attapulgite powder and the mixed acid solution were mixed at a solid-liquid ratio of 1:10 and placed in a reactor equipped with a condensation reflux device, and stirred at a constant temperature of 85℃ for 4h; after the reaction was completed, centrifugal separation was performed, and the filter cake was repeatedly washed with deionized water until the supernatant was neutral; the washed material was dried at 105℃ under blast drying until the weight was constant, to obtain acid-activated attapulgite;

[0096] b. The acid-activated attapulgite was laid flat in a crucible and placed in a muffle furnace, and heated from room temperature to 380℃ at a rate of 3℃ / min, and kept at this temperature for 2h;

[0097] This key temperature point of 380℃ is sufficient to remove most of the zeolite water and part of the structural water, causing the crystal structure of the attapulgite to "fold" and form more stable rigid pores, while avoiding the collapse of the pores and the sharp decrease in specific surface area caused by excessively high temperature.

[0098] After the heat treatment was completed, the furnace was naturally cooled to below 150℃, and then removed and immediately transferred to a desiccator to cool to room temperature, to obtain the heat-activated attapulgite;

[0099] c. The gamma-aminopropyltriethoxysilane is dissolved in 90% v / v aqueous ethanol solution, stirred for hydrolysis for 30 min, and prepared into a silane hydrolysis solution with a concentration of 2% v / v. The amino silane is selected, and after hydrolysis, the silanol bonds generated are condensed with the silicon hydroxyl groups on the surface of the attapulgite, firmly grafted; at the same time, the introduced amino groups (-NH2) are organic functional groups that can combine with organic acids, hormones, and other components in the rooting agent through hydrogen bonds or ionic bonds, playing the role of "anchoring" active ingredients.

[0100] The heat-activated attapulgite is added to the silane hydrolysis solution at a solid-liquid ratio of 1:8, and reacted at 60°C and 80-120 rpm for 5-6 h;

[0101] After the reaction is completed, the solid product is collected by centrifugation, washed with anhydrous ethanol three times to remove physically adsorbed silane, and finally dried at 80°C under vacuum for 6 h to obtain the modified attapulgite. Comparative Example 1

[0102] The formulation and preparation method are the same as in Example 2, but the citrus pectin oligosaccharide- spermidine complex is omitted, and its share is replaced by an equal amount of deionized water. Comparative Example 2

[0103] The formulation and preparation method are the same as in Example 2, but the citrus pectin oligosaccharide-spermidine complex is not prepared, but an equal amount of citrus pectin oligosaccharide and spermidine are simply physically mixed and added. Comparative Example 3

[0104] The formulation and preparation method are the same as in Example 2, but the brassinolide nanoemulsion is not used, and an equivalent brassinolide content of brassinolide soluble granules (dissolved with water and added) is directly added. Comparative Example 4

[0105] The formulation and preparation method are the same as in Example 2, but the modified attapulgite is replaced by unmodified natural attapulgite. Comparative Example 5

[0106] The formulation and preparation method are the same as in Example 2, but the surfactant system is replaced by an equal amount (0.4%) of Tween-80.

[0107] The rooting agents in Examples 1-3 and Comparative Examples 1-5 are tested, and the woody plant "red leaf photinia" current semi-lignified branches are selected as standard test materials. The cuttings are immersed in the rooting agents of each example and comparative example for 30 min, and the water treatment is used as a blank control (CK). The cuttings are inserted into a standardized seedling raising substrate and uniformly managed in an intelligent greenhouse. After 30 days, the rooting rate, average root number, and average root length are counted, and the root activity (TTC method) is measured, and the experimental results are shown in the following table:

[0108]

[0109] It should be understood that these embodiments are merely illustrative of the present application and should not be construed as limiting the scope of the present application. Furthermore, it should be understood that any references to prior art contained herein are not, and should not be taken as, constituting an admission that such prior art is widely known or forms part of the common general knowledge in the field of the present application.

Claims

1. A rooting agent for cuttings, characterized in that, It consists of the following raw materials by weight percentage: Citrus pectin oligosaccharide-spermidine complex: 5-15% Brassinolide nanoemulsion: 0.0001-0.0005% Proline: 2-5% Sugar alcohol calcium: 3-8% Modified attapulgite: 5-10% Surfactant: 0.25-0.55% Deionized water: Balance.

2. The rooting agent for cuttings as described in claim 1, characterized in that, The preparation method of the citrus pectin oligosaccharide-spermidine complex includes the following steps: A. Pectin was extracted from citrus peel and citrus pectin oligosaccharides with an average degree of polymerization of 3-7 were prepared by degradation using hydrogen peroxide-ascorbic acid method; B. Mix pectin oligosaccharides and spermidine at a mass ratio of 5:1 in a phosphate buffer solution with pH 7.5, add sodium cyanoborohydride as a reducing agent, and react at 55-60℃ for 5-6 hours. C. The reaction solution was purified by ultrafiltration with a molecular weight cutoff of 1000 Da, the filtrate was collected, and the solution was freeze-dried to obtain a white powdery citrus pectin oligosaccharide-spermidine complex.

3. The rooting agent for cuttings as described in claim 1, characterized in that: The preparation method of the brassinolide nanoemulsion includes the following steps: S1. Brassinolide is dissolved in soybean oil to form the oil phase; S2. Dissolve phospholipids and Tween-80 in deionized water to form the aqueous phase; S3. Under medium-speed stirring at 500-800 rpm, slowly add the oil phase of S1 dropwise to the aqueous phase of S2. After the addition is complete, use a high-speed shearing machine at a speed of 8,000-12,000 rpm for 5-10 minutes to form a crude emulsion. S4. The crude emulsion is passed through a high-pressure homogenizer and circulated 3-5 times at a pressure of 10,000-20,000 psi to obtain brassinolide nanoemulsion; The brassinolide nanoemulsion contains 0.5-1.5% w / w brassinolide, 7.5-13.5% w / w soybean oil, 8-15% w / w phospholipids, and 10-20% w / w Tween-80.

4. The rooting agent for cuttings as described in claim 1, characterized in that, The method for preparing the modified attapulgite includes the following steps: a. The natural attapulgite ore is crushed and ground, and then acidified with a mixture of hydrochloric acid and citric acid to obtain acid-activated attapulgite. b. Heat-treat acid-activated attapulgite to obtain thermally activated attapulgite; c. Add thermally activated attapulgite to silane hydrolysate and heat to react. After the reaction is complete, collect the solid product by centrifugation, wash with anhydrous ethanol and dry to obtain modified attapulgite.

5. The rooting agent for cuttings as described in claim 1, characterized in that: The surfactant is a mixture of tea saponin, alkyl polysaccharide and phosphatidylcholine, wherein tea saponin accounts for 0.1-0.3% w / w of the total weight of the hair rooting agent, alkyl polysaccharide accounts for 0.1-0.15% w / w of the total weight of the hair rooting agent, and phosphatidylcholine accounts for 0.05-0.1% w / w of the total weight of the hair rooting agent.

6. A method for preparing a rooting agent for cuttings as described in any one of claims 1-5, characterized in that, Includes the following steps: Ⅰ. Add 40-60% of the total formula amount of deionized water to the reactor, and then add proline, sugar alcohol calcium, and citrus pectin oligosaccharide-spermidine complex in sequence, and stir thoroughly to dissolve; II. Under high-speed stirring, first add modified attapulgite clay, stir and disperse, and then add brassinolide nanoemulsion; III. The surfactant is pre-stirred and mixed, dissolved in warm water, and then added to the reactor described above; IV. Add the remaining deionized water and continue stirring until a uniform and stable suspension is formed, which is the rooting agent for cuttings.

7. The method for preparing the rooting agent for cuttings as described in claim 6, characterized in that: When adding deionized water in step I, set the stirring speed to 80-100 rpm. After all raw materials have been added, increase the stirring speed to 180-200 rpm and continue stirring for 40-60 minutes. The dissolution temperature is 25-35℃.

8. The method for preparing the rooting agent for cuttings as described in claim 6, characterized in that: Before adding materials in step II, adjust the stirring speed to 350-400 rpm. After adding modified attapulgite clay, stir for 20-30 minutes and then add brassinolide nanoemulsion. After all materials are added, adjust the stirring speed to 550-600 rpm and continue stirring for 20-40 minutes. During the material addition process, control the temperature of the liquid to not exceed 40℃.

9. The method for preparing the rooting agent for cuttings as described in claim 6, characterized in that: In step III, the mass ratio of surfactant to warm water is 2.5-5.5:10, the temperature of the warm water is 40-45℃, and the stirring speed is 150-200 rpm.

10. The method for preparing the rooting agent for cuttings as described in claim 6, characterized in that: In step IV, the stirring speed is 120-150 rpm and the stirring time is 30-40 min.

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