Composition for Anti-graying hair and method to prepare thereof
The composition enhances melanin production in melanocytes by stimulating melanocyte activity, effectively reducing gray hair density and improving scalp health through a blend of natural extracts and compounds.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- ANVEYA LIVING PTE LTD
- Filing Date
- 2025-10-15
- Publication Date
- 2026-04-23
AI Technical Summary
Hair graying occurs due to the decrease in melanocyte activity and melanin production, primarily caused by oxidative stress, hormonal disruption, and malnutrition, leading to less pigmentation and visible gray or white hair.
A composition comprising Aloe barbadensis Leaf Extract, Camellia sinensis leaf extract, Rosmarinus officinalis Leaf Oil, Hamamelis virginiana extract, hyaluronic acid, panthenol, saccharide isomerate, alpha glucosyl hesperidin, dimethyl isosorbide, Neopicrorhiza scrophulariiflora root extract, mandarin peel paste, and N-acetyl L-tyrosine, along with excipients, is applied topically to stimulate melanocyte activity and enhance melanin production.
The composition significantly increases melanin content by 45.48% in melanocytes, promoting hair pigmentation and reducing gray hair density, while also improving scalp health and overall hair appearance.
Smart Images
Figure IN2025051663_23042026_PF_FP_ABST
Abstract
Description
[0001] COMPOSITION FOR ANTI-GRAYING HAIR AND METHOD TO PREPARE THEREOF
[0002] TECHNICAL FIELD
[0003] The present disclosure relates to a composition for anti-graying hair and a method to prepare thereof.
[0004] BACKGROUND
[0005] The graying of hair is one of the earliest and most noticeable signs of aging in humans. The phenomenon transcends cultural, geographic, and ethnic boundaries, holding deep social significance across the globe. As a result, there has been increasing interest in developing methods to reverse or slow down the process. Hair pigmentation, which gives rise to various hair colors such as black, brown, blonde, and red, is determined by the production of melanin, a pigment synthesized by melanocytes. These melanocytes are situated in the hair follicles and are responsible for producing two primary types of melanin-eumelanin and pheomelanin. The balance and ratio of these melanins, influenced by factors such as pH and cysteine levels within melanosomes, determine the resulting hair color.
[0006] Hair graying happens when melanocytes, the cells that produce melanin in hair follicles, gradually decrease. As people age, melanogenesis, the process of melanin production, slows down. Thus, leads to less melanin in the hair, causing it to turn gray or white. One major cause is oxidative stress, where reactive oxygen species (ROS) damage melanocytes. Psychological stress also speeds up graying by disrupting hormones, which affects melanocyte function. Malnutrition, particularly a lack of essential vitamins and minerals, further hampers melanin production.
[0007] Hair graying becomes more evident when melanogenesis stops, especially during the telogen phase of the hair cycle. Thus, results in fewer melanocytes and less melanin in the hair shaft, leading to gray or white hair. Gray hair contains fewer melanosomes (the structures that hold pigment), while white hair lacks them entirely.
[0008] Thus, there is need for a composition for anti-graying hair and a method to prepare thereof to overcome the aforementioned drawbacks. SUMMARY
[0009] In an aspect of the present disclosure, a composition for anti-graying of hair is disclosed. The composition comprising: Aloe barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition, Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition, Rosmarinus officinalis Leaf Oil in a range of 0.02 to 0.1% by volume of the composition, Hamamelis virginiana extract in a range of 0.5 to 2.00% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition, panthenol in a range of 0.2 to 2.00% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition, dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition, Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition, mandarin peel pastes in a range of 4.0 to 7.0% by volume of the composition, N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition, and one or more excipients.
[0010] In some aspects of the present disclosure, one or more excipients are selected from a group comprising: humectants, moisturizing agents, scalp hydrators, anti greying agents, preservatives, chelating agents, thickening and stabilizing agents, and conditioning agents.
[0011] In some aspects of the present disclosure, the humectants are selected from a group comprising: pentylene glycol in a range of 5.0 to 10.0% by volume of the composition, propanediol in a range of 1.00 to 3.00% by volume of the composition, and propylene glycol in a range of 1.0 to 3.0% by volume of the composition.
[0012] In some aspects of the present disclosure, the moisturising agents are selected from a group comprising: ethylhexylglycerin in a range of 0.2 to 0.6% by volume of the composition.
[0013] In some aspects of the present disclosure, the scalp hydrator is selected from a group comprising: gluconolactone in a range of 0.30 to 0.70% by volume of the composition. In some aspects of the present disclosure, the anti greying agent is biotin in a range of 0.01 to 0.1% by volume of the composition. In some aspects of the present disclosure, the thickening and stabilising agent is selected from a group comprising: acrylates / C10-30 alkyl acrylate crosspolymer in a range of 0.1 to 0.5% by volume of the composition.
[0014] In some aspects of the present disclosure, the chelating agent is selected from a group comprising: tetrasodium ethylenediamine tetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition.
[0015] In some aspects of the present disclosure, the conditioning agent is selected from a group comprising: polyalkylene glycol mono butyl ether in a range of 2.0 to 5.0% by volume of the composition.
[0016] In some aspects of the present disclosure, the preservatives are selected from a group comprising: phenoxyethanol in a range of 0.2 to 0.6% by volume of the composition, sodium benzoate in a range of 0.4 to 0.80% by volume of the composition, and potassium sorbate in a range of 0.4 to 0.80% by volume of the composition.
[0017] In an aspect of the present disclosure, a method of preparing the composition for antigraying hair is disclosed. The method comprising steps: adding demineralized (DM) water to a mixing vessel, followed by gradual addition of acrylates / cl0-30 alkyl acrylate crosspolymer in a range of 0.1 to 0.5% by volume of the composition, tetrasodium ethylenediamine tetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition, Aloe Barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition, dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition, and propanediol in a range of 1.00 to 3.00% by volume of the composition; preparing one or more premixes in separate vessels, and adding sequentially in the mixing vessel; and adding one or more ingredients propylene glycol in a range of 1.00-3.00 %, saccharide isomerate in a range of 0.1 -0.5 %, Hamamelis virginiana (Witch Hazel) in a range of 0.5-2.00 %, polyalkylene glycol mono butyl ether in a range of 2.00-5.00%, Rosmarinus officinalis Leaf Oil in a range of 0.02- 0.1 %, and ethylhexylglycerin and phenoxyethanol in a range of 0.2-0.6 % to form the composition.
[0018] In some aspects of the present disclosure, premix A comprises Sodium Benzoate in a range of 0.4 to 0.80% by volume of the composition and potassium sorbate in a range of 0.4 to 0.80% by volume of the composition, premix B comprises alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition, premix C comprises Biotin in a range of 0.01 to 0.1% by volume of the composition and Panthenol in a range of 0.2 to 2.00% by volume of the composition, premix D comprises Camellia sinensis Leaf Extract in a range of 0.05 to 0.30% by volume of the composition, premix E includes hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition, and premix F comprises Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition, mandarin peel paste in a range of 4.0 to 7.0% by volume of the composition, N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition, pentylene glycol in a range of 5.0 to 10.0% by volume of the composition, and gluconolactone in a range of 0.30 to 0.70% by volume of the composition.
[0019] In some aspects of the present disclosure, the composition is prepared in a topical form selected from a group comprising: serum, lotion, cream, gel, spray, ointment, emulsion, or oil.
[0020] In some aspects of the present disclosure, a method of treating a subject in need of hair anti-graying treatment, the method comprising: administering a composition to scalp of the subject, wherein the composition reduces the appearance of gray or white hair by stimulating melanocyte activity, enhancing melanin production, and promoting scalp health and hair pigmentation.
[0021] In some aspects of the present disclosure, the composition is administered in a dosage of 0.5 to 1.0 milliliters per application, once or twice daily.
[0022] In some aspects of the present disclosure, use of the composition, wherein the composition comprises- Aloe barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition, Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition, Rosmarinus officinalis Leaf Oil in a range of 0.02 to 0.1% by volume of the composition, Hamamelis virginiana extract in a range of 0.5 to 2.00% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition, panthenol in a range of 0.2 to 2.00% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition, dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition, Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition, mandarin peel pastes in a range of 4.0 to 7.0% by volume of the composition, N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition, and one or more excipients.
[0023] In some aspects of the present disclosure, use of a composition for reducing gray or white hair, restoring natural hair pigmentation, stimulating melanogenesis, and improving scalp condition and overall hair appearance in a subject in need thereof.
[0024] BRIEF DESCRIPTION OF DRAWINGS
[0025] The above and still further features and advantages of aspects of the present disclosure become apparent upon consideration of the following detailed description of aspects thereof, especially when taken in conjunction with the accompanying drawings, and wherein;
[0026] FIG. 1 illustrates a 45.48% increase in melanin content in the treated sample compared to the control.
[0027] To facilitate understanding, reference numerals have been used, where possible, to designate elements common to the figures.
[0028] DETAILED DESCRIPTION OF PREFERRED ASPECTS
[0029] This section is intended to provide an explanation and description of various possible aspects of the present disclosure. The aspects used herein, and the various features and advantageous details thereof are explained more fully with reference to non-limiting aspects illustrated in the accompanying drawing / s and detailed in the following description. The examples used herein are intended only to facilitate an understanding of ways in which the aspects may be practiced and to enable the person skilled in the art to practice the aspects used herein. Also, the examples / aspects described herein should not be construed as limiting the scope of the aspects herein.
[0030] Various aspects of the present disclosure provide a composition for anti-graying hair and a method to prepare thereof. The following description provides specific details of certain aspects of the disclosure illustrated in the drawings to provide a thorough understanding of those aspects. It should be recognized, however, that the present disclosure can be reflected in additional aspects and the disclosure may be practiced without some of the details in the following description.
[0031] The various aspects including the example aspects are now described more fully with reference to the accompanying drawings, in which the various aspects of the disclosure are shown. The disclosure may, however, be embodied in different forms and should not be construed as limited to the aspects set forth herein. Rather, these aspects are provided so that this disclosure is thorough and complete, and fully conveys the scope of the disclosure to those skilled in the art. In the drawings, the sizes of components may be exaggerated for clarity.
[0032] The subject matter of example aspects, as disclosed herein, is described specifically to meet statutory requirements. However, the description itself is not intended to limit the scope of this disclosure. Rather, the inventor / inventors have contemplated that the claimed subject matter might also be embodied in other ways, to include different features or combinations of features similar to the ones described in this document, in conjunction with other technologies. Generally, the various aspects including the example aspects relate to the composition for anti-graying hair and the method to prepare thereof.
[0033] In some aspects, the present disclosure provides the composition for anti-graying hair (hereinafter referred to and designated as “the composition”).
[0034] In some aspects of the present disclosure, the composition may include, but are not limited to, Aloe barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition, Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition, Rosmarinus officinalis Leaf Oil in a range of 0.02 to 0.1% by volume of the composition, Hamamelis virginiana extract in a range of 0.5 to 2.00% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition, panthenol in a range of 0.2 to 2.00% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition, dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition, Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition, mandarin peel pastes in a range of 4.0 to 7.0% by volume of the composition, N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition, and one or more excipients.
[0035] In some aspects of the present disclosure, the one or more excipients are selected from a group including humectants, moisturizing agents, scalp hydrators, anti greying agents, preservatives, chelating agents, thickening and stabilizing agents, and conditioning agents. Aspects of the present disclosure are intended to include or otherwise cover any type of excipients including known, related art, and / or without deviating from the scope of the present disclosure.
[0036] In some aspects of the present disclosure, the humectants are selected from a group including pentylene glycol in a range of 5.0 to 10.0% by volume of the composition, propanediol in a range of 1.00 to 3.00% by volume of the composition, and propylene glycol in a range of 1.0 to 3.0% by volume of the composition.
[0037] In some aspects of the present disclosure, the moisturising agents are selected from a group including ethylhexylglycerin in a range of 0.2 to 0.6% by volume of the composition.
[0038] In some aspects of the present disclosure, the scalp hydrator is selected from a group including gluconolactone in a range of 0.30 to 0.70% by volume of the composition.
[0039] In some aspects of the present disclosure, the anti greying agent is biotin in a range of 0.01 to 0.1% by volume of the composition.
[0040] In some aspects of the present disclosure, the thickening and stabilising agent is selected from a group including acrylates / C10-30 alkyl acrylate crosspolymer in a range of 0.1 to 0.5% by volume of the composition.
[0041] In some aspects of the present disclosure, the chelating agent is selected from a group including tetrasodium ethylenediamine tetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition. In some aspects of the present disclosure, the conditioning agent is selected from a group comprising: polyalkylene glycol mono butyl ether in a range of 2.0 to 5.0% by volume of the composition.
[0042] In some aspects of the present disclosure, the preservatives are selected from a group comprising: phenoxyethanol in a range of 0.2 to 0.6% by volume of the composition, sodium benzoate in a range of 0.4 to 0.80% by volume of the composition, and potassium sorbate in a range of 0.4 to 0.80% by volume of the composition.
[0043] In some aspects of the present disclosure, Aloe barbadensis leaf extract may be used as a moisturizing agent to hydrate scalp tissues.
[0044] In some aspects of the present disclosure, Camellia sinensis leaf extract may be used as an anti-greying agent by protecting hair follicles from oxidative damage.
[0045] In some aspects of the present disclosure, Rosmarinus officinalis leaf oil may be used as an anti-greying agent to support melanin production and delay hair depigmentation.
[0046] In some aspects of the present disclosure, Hamamelis virginiana extract may be used as an antibacterial agent to maintain scalp hygiene and reduce microbial load.
[0047] In some aspects of the present disclosure, hyaluronic acid may be used as a moisturizing agent to enhance scalp hydration and improve hair texture.
[0048] In some aspects of the present disclosure, panthenol may be used as a moisturizing agent to condition hair strands and improve hair elasticity.
[0049] In some aspects of the present disclosure, saccharide isomerate may be used as a moisturizing agent to provide long-lasting hydration to the scalp.
[0050] In some aspects of the present disclosure, alpha glucosyl hesperidin may be used as an antioxidant to reduce oxidative stress on scalp tissues.
[0051] In some aspects of the present disclosure, dimethyl isosorbide may be used as a moisturizing agent to enhance penetration of active ingredients into the scalp.
[0052] In some aspects of the present disclosure, Neopicrorhiza scrophulariiflora root extract may be used as an anti-greying agent to support melanin biosynthesis and delay hair color loss. In some aspects of the present disclosure, mandarin peel paste may be used as an antioxidant to neutralize free radicals and promote scalp health.
[0053] In some aspects of the present disclosure, N-acetyl L-tyrosine may be used as a melanin-enhancing agent to stimulate pigmentation in hair follicles.
[0054] In some aspects of the present disclosure, a method of preparing the composition may include steps: adding demineralized (DM) water to a mixing vessel, followed by the gradual addition of acrylates / cl0-30 alkyl acrylate crosspolymer may be in a range of 0.1 -0.5 %, tetrasodium ethylenediamine tetraacetic acid (EDTA) may be in a range of 0.01-0.1 %, Aloe Barbadensis leaf extract may be in a range of 0.05-0.20 %, dimethyl isosorbide may be in a range of 0.5-2.00 %, and propanediol may be in a range of 1.00- 3.00 %. The ingredients may be thoroughly mixed and homogenized to ensure uniformity and consistency in the base formulation; preparing one or more premixes (A to F) in a separate vessel and adding to the mixing vessel sequentially; the Premix A may be prepared by dissolving sodium benzoate may be in a range of 0.4-0.80 % and potassium sorbate may be in a range of 0.4-0.80 % in DM water, premix B may be prepared by dissolving alpha glucosyl hesperidin may be in a range of 0.1 -0.5 % to DM water, premix C may be prepared by dissolving biotin may be in a range of 0.01-0.1 % and panthenol may be in a range of 0.2-2.00 % in the DM water, premix D may be prepared by dissolving Camellia Sinensis Leaf Extract may be in a range of 0.05- 0.30 % with the DM water, premix E may be prepared by dissolving hyaluronic acid may be in a range of 0.01-0.1 % by with DM water, and premix F may be prepared by dissolving Neopicrorhiza scrophulariiflora root extract may be in a range of 2.0-4.0%, mandarin peel paste may be in a range of 4.0-7.0 %, N-acetyl L-tyrosine may be in a range of 3.0-6.0 %, pentylene glycol may be in a range of 5.0-10.0 %, and gluconolactone may be in a range of 0.30-0.70 % is dissolved in DM water; and adding propylene glycol may be in a range of 1.00-3.00 %, saccharide isomerate may be in a range of 0.1 -0.5 %, Hamamelis virginiana (Witch Hazel) may be in a range of 0.5- 2.00 %, polyalkylene glycol mono butyl ether may be in a range of 2.00-5.00%, Rosmarinus officinalis leaf oil may be in a range of 0.02-0.1 %, and ethylhexylglycerin and phenoxyethanol may be in a range of 0.2-0.6 % to form the composition. In some aspects of the present disclosure, the composition may be prepared in a topical form selected from a group including serum, lotion, cream, gel, spray, ointment, emulsion, or oil.
[0055] In some aspects of the present disclosure, a method of treating a subject in need of hair anti-graying treatment is disclosed. The method includes administering the composition to scalp of the subject, such that the composition reduces the appearance of gray or white hair by stimulating melanocyte activity, enhancing melanin production, and promoting scalp health and hair pigmentation.
[0056] In some aspects of the present disclosure, the composition is administered in a dosage of 0.5 to 1.0 milliliters per application, once or twice daily.
[0057] In some aspects of the present disclosure, use of the composition, wherein the composition comprises- Aloe barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition, Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition, Rosmarinus officinalis Leaf Oil in a range of 0.02 to 0.1% by volume of the composition, Hamamelis virginiana extract in a range of 0.5 to 2.00% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition, panthenol in a range of 0.2 to 2.00% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition, dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition, Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition, mandarin peel pastes in a range of 4.0 to 7.0% by volume of the composition, N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition, and one or more excipients.
[0058] In some aspects of the present disclosure, use of a composition for reducing gray or white hair, restoring natural hair pigmentation, stimulating melanogenesis, and improving scalp condition and overall hair appearance in a subject in need thereof.
[0059] Examples:
[0060] The disclosure will now be illustrated with working examples and study, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed composition. It is to be understood that this disclosure is not limited to methods, and experimental conditions described, as such methods and conditions may vary.
[0061] EXAMPLE 1
[0062] Demineralized (DM) water was added to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer (0.23%), Tetrasodium Ethylenediamine Tetraacetic Acid (EDTA) (0.075%), Aloe barbadensis Leaf Extract (0.10%), Dimethyl Isosorbide (1.5%), and Propanediol (1.75%). These ingredients were mixed and homogenized to ensure uniformity. Further, one or more premixes (A to F) were prepared separately, each with DM water and specific ingredients. Premix A contains Sodium Benzoate (0.65%) and Potassium Sorbate (0.60%); Premix B contains Alpha Glucosyl Hesperidin (0.25%); Premix C includes Biotin (0.05%) and Panthenol (0.75%); Premix D contains Camellia sinensis Leaf Extract (0.10%); Premix E contains Hyaluronic Acid (0.02%); and Premix F contains Neopicrorhiza scrophulariiflora Root Extract (2.50%), Mandarin Peel Paste (4.50%), N-Acetyl L- Tyrosine (4.80%), Pentylene Glycol (7.50%), and Gluconolactone (0.55%). The premixes were added sequentially to the main mixture. Finally, remaining ingredients such as Propylene Glycol (1.80%), Saccharide Isomerate (0.30%), Hamamelis virginiana (Witch Hazel, 1.00%), Polyalkylene Glycol Mono Butyl Ether (3.50%), Rosmarinus officinalis Leaf Oil (0.075%), and Ethylhexylglycerin-Phenoxy ethanol (0.35%) were incorporated. The mixture is stirred thoroughly until a homogenized final composition is achieved.
[0063] Each and every ingredient <plant extract, oil, chemicals, cells lines> of the claimed composition are commercially procured from India.
[0064] EXAMPLE 2 Demineralized (DM) water was added to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer (0.40%), Tetrasodium Ethylenediamine Tetraacetic Acid (EDTA) (0.10%), Aloe barbadensis Leaf Extract (0.18%), Dimethyl Isosorbide (2.00%), and Propanediol (3.00%). These ingredients were mixed and homogenized to ensure uniformity. Further, one or more premixes (A to F) were prepared separately, each with DM water and specific ingredients. Premix A contains Sodium Benzoate (0.80%) and Potassium Sorbate (0.75%); Premix B contains Alpha Glucosyl Hesperidin (0.50%); Premix C includes Biotin (0.095%) and Panthenol (1.5%); Premix D contains Camellia sinensis Leaf Extract (0.20%); Premix E contains Hyaluronic Acid (0.10%); and Premix F contains Neopicrorhiza scrophulariiflora Root Extract (3.80%), Mandarin Peel Paste (5.50%), N-Acetyl L- Tyrosine (5.80%), Pentylene Glycol (9.00%), and Gluconolactone (0.70%). The premixes were added sequentially to the main mixture. Finally, remaining ingredients such as Propylene Glycol (2.80%), Saccharide Isomerate (0.50%), Hamamelis virginiana (Witch Hazel, 2.00%), Polyalkylene Glycol Mono Butyl Ether (4.50%), Rosmarinus officinalis Leaf Oil (0.10%), and Ethylhexylglycerin-Phenoxy ethanol (0.50%) were incorporated. The mixture is stirred thoroughly until a homogenized final composition is achieved.
[0065] Each and every ingredient <plant extract, oil, chemicals, cells lines> of the claimed composition are commercially procured from India.
[0066] Overall Working:
[0067] The study was conducted to evaluate the effectiveness of claimed composition designed to support natural hair pigmentation by enhancing melanin production in hair follicle-derived cells. The investigation focused on the ability of the claimed composition to stimulate pigment synthesis within melanocytes, the cells responsible for hair color, and to assess early biological activity following short exposure periods. Results demonstrated a 45.48 percent increase in intracellular pigment synthesis within 48 hours of treatment, indicating rapid activation of melanogenic pathways. The induction of melanin occurred within a short in-vitro exposure time, establishing that the claimed composition initiates biological responses that precede visible repigmentation at the microscopic level.
[0068] A direct comparison between treated cells < claimed composition> and vehicle-treated controls < claimed composition absent> confirmed the causal effect of the composition on melanin production. This controlled assessment validated the composition’s ability to enhance melanocyte activity, supporting its potential application in cosmetic or therapeutic formulations aimed at preventing or reversing hair greying.
[0069] The serum increased the Absorbance (106.36), which is a "direct indicator of melanin concentration", by 45.48% which is a strong result at the cellular level even before clinical results are visible. However, a vehicle control showed no such improvement (with Absorbance of only 73.11), confirming that the boost is due to the active serum.
[0070] Clinical Trial Study:
[0071] The study was conducted with the objective of evaluating the anti-greying effect of the composition by assessing the ability to enhance melanin content in melanocyte cells derived from hair follicle cells. Melanin is the primary pigment responsible for hair color, and its production in melanocytes is crucial for maintaining hair pigmentation. The experiment was aimed to quantify the melanin content following treatment with the composition and assess its potential in reversing or preventing hair greying.
[0072] Materials and Methods
[0073] Cell Line and Culture Conditions
[0074] The study utilized WM 266-4 melanocyte cells Commercially procured from Inida> derived from hair follicle cells. The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with:
[0075] • 2 millimolar L-glutamine.
[0076] • 10% fetal bovine serum (FBS).
[0077] • Antibiotic-antimycotic solution containing 1000 milligrams per milliliter streptomycin sulfate, 1000 units per milliliter penicillin G sodium, and 2.5 milligrams per milliliter amphotericin B. • The cells were maintained in a humidified incubator at 37°C with 5% CO2 until they reached 70-80% confluency.
[0078] All material required in experiments were commercially procured from India.
[0079] Preparation of the Composition
[0080] The test composition was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock concentration of 50% v / v. For cell treatment, a working solution of 5% v / v was prepared.
[0081] Treatment Protocol
[0082] The WM 266-4 cells (2 x 105) were seeded on 60 mm cell culture plates containing DMEM medium supplemented with 10% FBS and incubated at 37°C in a 5% CO2 atmosphere. The cells were further incubated for 48 hours, after which they were trypsinized, and the resulting cell suspension was pelleted by centrifugation at 10,000 rpm for 10 minutes. The cell pellet was then resuspended in 500 pl of RIP A buffer, followed by sonication for 10 minutes to prepare the lysate. The protein concentration of the cell lysate was subsequently estimated using the Bradford method.
[0083] Assay for Melanin Content
[0084] Standard Preparation- A stock solution of melanin was prepared at a concentration of 5 mg / mL in DMSO. The melanin stock solution was then diluted with assay buffer to obtain a 500 pg / mL melanin standard solution (Ml). A series of 1:2 serial dilutions was subsequently performed to generate the remaining melanin standards (M2 to M7). Sample and Standard Assay- Each sample well was loaded with 250 micrograms of protein lysate, and the volume was adjusted to 50 microliters using assay buffer. Subsequently, 50 microliters of signal enhancer were added to each well, and the plate was incubated at room temperature for 30 to 60 minutes.
[0085] Fluorescence Measurement
[0086] Fluorescence intensity was measured using a plate reader at: A,EX / AEHI = 470 / 550 nm
[0087] • ^Excitation: 470 nm
[0088] • ^Emission: 550 nm
[0089] • Cutoff: 515 nm Results and Calculations
[0090] The percentage change in melanin content was calculated using the formula: f,.. , ,, Cnanee where:
[0091] ACOntroi= 73.11 (absorbance of vehicle-treated control)
[0092] Atreated= 106.36 (absorbance of treated sample)
[0093] % change in melanin content = [(73.11 -106.36) / 73.11 ] x 100
[0094] This result indicated a 45.48% increase in melanin content upon treatment with the composition.
[0095] MELANOCYTE ACTIVITY: 45.48% direct increase in mechanistic intracellular pigment synthesis in melanocytes within 48 hrs. Melanin induction occurs within short in-vitro exposure times, establishing early biological activity that precedes microscopic repigmentation. Direct controlled comparison (treated vs vehicle) proves causal induction of melanin (+45.48%). The serum increased the Absorbance (106.36), which is a "direct indicator of melanin concentration", by 45.48% which is a strong result at the cellular level even before clinical results are visible. In contrast, a vehicle control showed no such improvement (with Absorbance of only
[0096] 73.11), confirming that the boost is due to the active serum.
[0097] Fig. 1 illustrates the effect of a treated sample on melanin content in comparison to a vehicle-treated control, using absorbance as an indirect measure of melanin levels where higher absorbance reflects greater melanin presence. The control sample showed an absorbance of 73.11, while the treated sample showed a higher absorbance of 106.36. This increase corresponds to a 45.48% rise in melanin content, calculated using the formula: % change = [(Actr - Atreated) / Actr] x 100. The bar graph visually reinforces this finding, with the treated sample displaying a noticeably higher bar than the control. Fig. 1 indicate that the treatment significantly enhances melanin production compared to the control.
[0098] Observation and Conclusion
[0099] The results demonstrated that treatment with the composition significantly increased melanin content in WM 266-4 melanocyte cells. The observed 45.48% elevation in melanin production, compared to the untreated control, supported the hypothesis that the present composition stimulates melanogenic activity in hair follicle melanocytes.
[0100] These findings suggest that the composition may effectively restore hair pigmentation and counteract hair greying. The study confirms the potential application of the composition in cosmetic or therapeutic formulations aimed at managing premature hair greying by enhancing melanocyte activity and melanin biosynthesis.
[0101] Certain advantages of the composition of the present disclosure are listed hereinbelow:
[0102] • The composition shows a significant reduction in gray hair density which enhance melanogenesis and boost melanin production, leading to repigmentation of hair.
[0103] • The combination of Rosemary’s phenolic compounds, such as rosmarinic acid, provides a shield against oxidative stress, a major contributor to premature graying.
[0104] • In addition to reducing gray hair, the composition reduces scalp issues such as itchiness, dryness, and redness, enhancing overall scalp health.
[0105] • The inclusion of natural compounds in the composition provides a sustainable and biocompatible solution for treating gray hair.
[0106] • The ingredients such as Neopicrorhiza scrophulariiflora root extract and Rosemary Oil have been shown to stimulate hair follicles and promote hair growth.
[0107] • The advanced hair melanin-synthesizing ingredients, including Acetyl Tyrosine, help maintain natural hair color and prevent premature graying. • The ingredients such as Aloe barbadensis Extract, Panthenol, and Hyaluronic acid provide hydration and nourishment to the hair and scalp.
[0108] • Antioxidants from Camellia Sinensis Leaf Extract help protect hair from oxidative stress and environmental damage.
[0109] The foregoing discussion of the present disclosure has been presented for purposes of illustration and description. It is not intended to limit the present disclosure to the form or forms disclosed herein. In the foregoing Detailed Description, for example, various features of the present disclosure are grouped together in one or more aspects, configurations, or aspects for the purpose of streamlining the disclosure. The features of the aspects, configurations, or aspects may be combined in alternate aspects, configurations, or aspects other than those discussed above. This method of disclosure is not to be interpreted as reflecting an intention the present disclosure requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed aspect, configuration, or aspect. Thus, the following claims are hereby incorporated into this Detailed Description, with each claim standing on its own as a separate aspect of the present disclosure.
[0110] Moreover, though the description of the present disclosure has included description of one or more aspects, configurations, or aspects and certain variations and modifications, other variations, combinations, and modifications are within the scope of the present disclosure, e.g., as may be within the skill and knowledge of those in the art, after understanding the present disclosure. It is intended to obtain rights which include alternative aspects, configurations, or aspects to the extent permitted, including alternate, interchangeable and / or equivalent structures, functions, a range or steps to those claimed, whether or not such alternate, interchangeable and / or equivalent structures, functions, ranges or steps are disclosed herein, and without intending to publicly dedicate any patentable subject matter.
Claims
We Claim1. A composition for anti-graying of hair, the composition comprising:• Aloe barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition,• Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition,• Rosmarinus officinalis Leaf Oil in a range of 0.02 to 0.1% by volume of the composition,• Hamamelis virginiana extract in a range of 0.5 to 2.00% by volume of the composition,• hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition,• panthenol in a range of 0.2 to 2.00% by volume of the composition,• saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition,• alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition,• dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition,• Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition,• mandarin peel pastes in a range of 4.0 to 7.0% by volume of the composition,• N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition,• one or more excipients, and• water.
2. The composition as claimed in claim 1, wherein the one or more excipients are selected from a group comprising humectants, moisturizing agents, scalp hydrators,anti greying agents, preservatives, chelating agents, thickening and stabilizing agents, and conditioning agents.
3. The composition as claimed in claim 2, wherein the humectants are selected from a group comprising pentylene glycol in a range of 5.0 to 10.0% by volume of the composition, propanediol in a range of 1.00 to 3.00% by volume of the composition, and propylene glycol in a range of 1.0 to 3.0% by volume of the composition.
4. The composition as claimed in claim 2, wherein the moisturising agents are selected from a group comprising ethylhexylglycerin in a range of 0.2 to 0.6% by volume of the composition.
5. The composition as claimed in claim 2, wherein the scalp hydrator is selected from a group comprising: gluconolactone in a range of 0.30 to 0.70% by volume of the composition.
6. The composition as claimed in claim 2, wherein the anti greying agent is biotin in a range of 0.01 to 0.1% by volume of the composition.
7. The composition as claimed in claim 2, wherein the thickening and stabilising agent is selected from a group comprising acrylates / C 10-30 alkyl acrylate crosspolymer in a range of 0.1 to 0.5% by volume of the composition.
8. The composition as claimed in claim 2, wherein the chelating agent is selected from a group comprising tetrasodium ethylenediamine tetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition.
9. The composition as claimed in claim 2, wherein the conditioning agent is selected from a group comprising polyalkylene glycol mono butyl ether in a range of 2.0 to 5.0% by volume of the composition.
10. The composition as claimed in claim 2, wherein the preservatives are selected from a group comprising phenoxyethanol in a range of 0.2 to 0.6% by volume of thecomposition, sodium benzoate in a range of 0.4 to 0.80% by volume of the composition, and potassium sorbate in a range of 0.4 to 0.80% by volume of the composition.
11. A method of preparing the composition for anti-graying hair serum, the method comprising: a. adding demineralized (DM) water to a mixing vessel, followed by gradual addition of aery lates / cl 0-30 alkyl acrylate crosspolymer in a range of 0.1 to 0.5% by volume of the composition, tetrasodium ethylenediamine tetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition, Aloe Barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition, dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition, and propanediol in a range of 1.00 to 3.00% by volume of the composition; b. preparing one or more premixes in separate vessels, and adding sequentially in the mixing vessel; and c. adding propylene glycol in a range of 1.00-3.00 %, saccharide isomerate in a range of 0.1-0.5 %, Hamamelis virginiana (witch hazel) in a range of 0.5- 2.00 %, polyalkylene glycol mono butyl ether in a range of 2.00-5.00%, Rosmarinus officinalis Leaf Oil in a range of 0.02-0.1 %, and ethylhexylglycerin and phenoxyethanol in a range of 0.2-0.6 % to form the composition.
12. The method as claimed in claim 11, wherein: a. premix A comprises sodium benzoate in a range of 0.4 to 0.80% by volume of the composition and potassium sorbate in a range of 0.4 to 0.80% by volume of the composition, b. premix B comprises alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition, c. premix C comprises biotin in a range of 0.01 to 0.1% by volume of the composition and panthenol in a range of 0.2 to 2.00% by volume of the composition,d. premix D comprises Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition, e. premix E comprises hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition, and f. premix F comprises Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition, mandarin peel paste in a range of 4.0 to 7.0% by volume of the composition, and n-acetyl 1-tyrosine in a range of 3.0 to 6.0% by volume of the composition.
13. The method as claimed in claim 11, wherein the composition is prepared in a topical form selected from a group comprising: serum, lotion, cream, gel, spray, ointment, emulsion, or oil.
14. A method of treating a subject in need of hair anti-graying treatment, the method comprising: administering a composition to scalp of the subject, wherein the composition reduces the appearance of gray or white hair by stimulating melanocyte activity, enhancing melanin production, and promoting scalp health and hair pigmentation.
15. The method as claimed in claim 14, wherein the composition is administered in a dosage of 0.5 to 1.0 milliliters per application, once or twice daily.
16. Use of a composition for reducing gray or white hair, restoring natural hair pigmentation, stimulating melanogenesis, and improving scalp condition and overall hair appearance in a subject in need thereof.
17. The use of the composition as claimed in claim 16, wherein the composition comprises-• Aloe barbadensis Leaf Extract in a range of 0.05 to 0.20% by volume of the composition,• Camellia sinensis leaf extract in a range of 0.05 to 0.30% by volume of the composition,• Rosmarinus officinalis Leaf Oil in a range of 0.02 to 0.1% by volume of the composition,• Hamamelis virginiana extract in a range of 0.5 to 2.00% by volume of the composition,• hyaluronic acid in a range of 0.01 to 0.1% by volume of the composition,• panthenol in a range of 0.2 to 2.00% by volume of the composition,• saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition,• alpha glucosyl hesperidin in a range of 0.1 to 0.5% by volume of the composition,• dimethyl isosorbide in a range of 0.5 to 2.00% by volume of the composition,• Neopicrorhiza scrophulariiflora root extract in a range of 2.0 to 4.0% by volume of the composition,• mandarin peel pastes in a range of 4.0 to 7.0% by volume of the composition,• N-acetyl L-tyrosine in a range of 3.0 to 6.0% by volume of the composition, and• one or more excipients.