Cutting propagation method for sinojackia xylocarpa

Abstract

The invention discloses a cutting propagation method for sinojackia xylocarpa, and belongs to the technical field of plant asexual propagation. The cutting propagation method of the sinojackia xylocarpa comprises the following steps: immersing cutting slips of the sinojackia xylocarpa into an ethylicin solution, and then inserting the cutting slips into a cutting medium for cultivation. By means of the cutting propagation method, the cutting rooting rate of the twigs of the sinojackia xylocarpa can be remarkably increased by controlling the cutting time, the cutting environment, the cutting treatment, the cutting medium and other factors, the highest cutting rooting rate after two months can reach 90%, and the highest cutting rooting rate after three months can reach 80% or above. The cutting propagation method provided by the invention is simple and rapid, low in cost and easy to implement on a large scale.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of plant asexual propagation, and particularly relates to a cutting propagation method of Sinojackia rehderiana Hu. BACKGROUND

[0002] Sinojackia rehderiana Hu belongs to the Sinojackia of the Styracaceae, and the Sinojackia is the first new genus published by Chinese botanists and is also a few-species genus specific to China. The type specimen of Sinojackia rehderiana Hu was collected in Wangcheng Township, Nanchang City, Jiangxi Province, China, and has now become extinct in the type specimen habitat. Sinojackia rehderiana Hu is mostly a deciduous tree or shrub with pure white flowers and a unique flower shape like a steelyard, and has extremely high ornamental value, but its distribution is intermittent and fragmented. In fact, due to the fact that the seeds of Sinojackia rehderiana Hu are lignified endocarp, have the characteristics of deep dormancy, and need 2-3 years to break dormancy in the natural environment, and some even germinate in the fourth year, it is very difficult to rely on natural regeneration by seeds, and the habitat suitable for Sinojackia rehderiana Hu is severely destroyed, so Sinojackia rehderiana Hu has become an endangered protected tree species.

[0003] Cutting is a simple and effective asexual propagation method, which is very suitable for the breeding and protection method of endangered tree species, and its advantages not only lie in fast propagation speed, but also in maintaining excellent characteristics. How to develop a high-survival-rate asexual propagation method suitable for Sinojackia rehderiana Hu is a technical problem to be solved in the field. SUMMARY

[0004] The purpose of the present application is to provide a cutting propagation method of Sinojackia rehderiana Hu. Through experimental research on the cutting propagation technology of Sinojackia rehderiana Hu, ethion is selected as a hormone to soak cuttings, different materials of cutting substrates are used, and naphthalene acetic acid (NAA) commonly used for cutting is compared, so as to find out the best cutting method of Sinojackia rehderiana Hu, further optimize the cutting rooting technology, and improve the seedling quality.

[0005] To achieve the above purpose, the present application provides the following technical scheme:

[0006] One of the technical schemes of the present application: a cutting propagation method of Sinojackia rehderiana Hu is provided, which comprises the following steps: immersing the cuttings of Sinojackia rehderiana Hu in an ethion solution, and then inserting them into a cutting substrate for cultivation.

[0007] The allitridin used in the application for promoting the rooting of the narrowfruit tree cutting and increasing the survival rate of the cutting has the chemical name of ethyl thioethyl sulfonate, and has the similar structure and function to natural allicin. The allitridin has the strong garlic and acetic acid smell, belongs to the plant source pesticide, and is mainly obtained by artificial synthesis at present. The allitridin is generally used as the high-efficiency broad-spectrum and environment-friendly bactericide in agriculture, and the bactericidal effect is mainly realized through the sulfide bond (-S-S-) in the molecular structure. Specifically, when the liquid medicine of the allitridin contacts the cells of the pathogenic bacteria, the sulfide bond can penetrate the cell membrane of the pathogenic bacteria, and reacts with the substances containing -SH (mercapto) in the bacterial molecules (such as cysteine and glutathione); the -SH group is the active center of many biological enzymes (such as dehydrogenase and respiration-related enzymes), and after the allitridin combines with the -SH group, the enzymes lose the activity, thereby interrupting the normal metabolism and respiration of the pathogenic bacteria; when the metabolism and respiration are inhibited, the pathogenic bacteria cells cannot obtain energy, the growth and reproduction are suppressed, and finally the cells die. Different from the conventional application, the allitridin is selected as the hormone for promoting the rooting of the narrowfruit tree cutting and increasing the survival rate of the cutting, and the application is the first of its kind.

[0008] Preferably, the cutting is the new semi-lignified tender branch of the 1-2 year old narrowfruit tree.

[0009] More preferably, the length of the cutting is 5-10 cm, and the uppermost part of the cutting retains one leaf cut by half.

[0010] Preferably, the concentration of the allitridin solution is 500 mg / L, and the time for the cutting to be immersed in the allitridin solution is 1 h.

[0011] Preferably, the cultivation substrate is vermiculite.

[0012] Preferably, the cultivation environment is a sunshade greenhouse with the relative humidity of 80±10% and the light transmittance of 60-70%.

[0013] Preferably, the cutting time is in the middle and late June.

[0014] Preferably, the cutting further comprises a disinfection step after the cutting is inserted into the cutting substrate.

[0015] More preferably, the disinfection uses the carbendazim solution diluted by 500 times.

[0016] The beneficial technical effects of the application are as follows:

[0017] By controlling the cutting time, cutting environment, cutting treatment, cutting substrate and other factors, the cutting propagation method of the application can significantly improve the rooting rate of the narrowfruit tree tender branch cutting. The rooting rate of the cutting after two months can be up to 90%, and the rooting rate of the cutting after three months can be up to more than 80%.

[0018] The cutting propagation method is simple, rapid, low in cost and easy to implement on a large scale. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description only constitute some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative effort.

[0020] Figure 1 Figure 1 is a photograph of the above-ground growth form of a cutting seedling of Gomphocarpus physocarpus obtained 20 days after cutting for Example 1;

[0021] Figure 2 Figure 2 is a photograph of the above-ground growth form of a cutting seedling of Gomphocarpus physocarpus obtained 40 days after cutting for Example 1;

[0022] Figure 3 Figure 3 is a photograph of the above-ground growth form of a cutting seedling of Gomphocarpus physocarpus obtained 60 days after cutting for Example 1;

[0023] Figure 4 Figure 4 is a photograph of the root system growth form of a cutting seedling of Gomphocarpus physocarpus obtained 60 days after cutting for Example 1;

[0024] Figure 5 Figure 5 is a photograph of the root system growth form of a cutting seedling of Gomphocarpus physocarpus obtained 90 days after cutting for Example 1. DETAILED DESCRIPTION

[0025] The various illustrative embodiments of the present application will now be described in detail in connection with the accompanying drawings. This description is made for illustrative purposes only and is not intended to limit the present application. The present application is described in connection with certain embodiments, and it will be understood that the terms used herein are merely descriptive, but are not intended to limit the present application.

[0026] It should be noted that the present application does not describe in detail the conventional means in the art, and is not the focus of the present application.

[0027] In addition, for the numerical range in the present application, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intermediate value in the stated range, and any other stated value or intermediate value in the stated range, is also included in the present application. The upper limit and the lower limit of these smaller ranges can be independently included or excluded from the range.

[0028] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar to or equivalent to those described herein may be used in the implementation or testing of this invention.

[0029] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0030] Example 1

[0031] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0032] (1) Select a shaded greenhouse with good ventilation from north to south, with a light transmittance of 60-70%, and set up timed spraying to keep the relative humidity of the greenhouse at 80±10%. The cutting time is June 20th in summer.

[0033] (2) Select new semi-lignified tender branches of 1-2 year old narrow-fruited cypress trees as cuttings. Cut the cuttings to a length of 5-10 cm, retain one small leaf at the top, and cut them in half.

[0034] (3) The cutting substrate should be vermiculite with a size of 3~6mm, and the hormone should be a solution of ethionine with a concentration of 500mg / L.

[0035] (4) Soak the cuttings in the prepared allicin solution for 1 hour, and then insert half of the cuttings obliquely into the substrate.

[0036] (5) After the cuttings are taken, turn on the timed misting system and spray continuously for 8 hours a day to keep the cutting shed moist (relative humidity 80±10%). Then disinfect with a 500-fold diluted carbendazim solution once a day for one week. After that, carry out routine culture.

[0037] Figure 1 The image shows the above-ground growth morphology of *Sterculia lanceolata* cuttings obtained 20 days after cutting in Example 1.

[0038] Figure 2 This is a photograph of the above-ground growth morphology of *Symplocos nephrolepis* cuttings obtained 40 days after cutting in Example 1.

[0039] Figure 3 The above-ground growth morphology of *Symplocos nephrolepis* cuttings obtained 60 days after cutting in Example 1 is shown in the photograph.

[0040] Figure 4 Photographs showing the root growth morphology of *Sterculia lanceolata* cuttings obtained 60 days after cutting in Example 1.

[0041] Figure 5This is a photograph of the root growth morphology of *Symplocos nephrolepis* cuttings obtained 90 days after cutting in Example 1.

[0042] Example 2

[0043] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0044] Compared with Example 1, the difference is that the concentration of the allicin solution was adjusted to 1000 mg / L, while the other conditions were the same as in Example 1.

[0045] Example 3

[0046] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0047] Compared with Example 1, the difference is that the concentration of the allicin solution was adjusted to 1500 mg / L, while the other conditions were the same as in Example 1.

[0048] Example 4

[0049] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0050] Compared with Example 1, the difference is that the cutting substrate is selected as 10mm peat moss, while other conditions are the same as in Example 1.

[0051] Example 5

[0052] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0053] Compared with Example 4, the difference is that the concentration of the allicin solution was adjusted to 1000 mg / L, while the other conditions were the same as in Example 1.

[0054] Example 6

[0055] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0056] Compared with Example 4, the difference is that the concentration of the allicin solution was adjusted to 1500 mg / L, while the other conditions were the same as in Example 1.

[0057] Example 7

[0058] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0059] Compared with Example 1, the difference is that the cutting substrate is selected with a volume ratio of 1:2 river sand (0.5mm) and perlite (2mm), while other conditions are the same as in Example 1.

[0060] Example 8

[0061] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0062] Compared with Example 7, the difference is that the concentration of the allicin solution was adjusted to 1000 mg / L, while the other conditions were the same as in Example 1.

[0063] Example 9

[0064] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0065] Compared with Example 7, the difference is that the concentration of the allicin solution was adjusted to 1500 mg / L, while the other conditions were the same as in Example 1.

[0066] Three months after implementing the asexual propagation methods of Examples 1-9, the rooting rate of the *Symplocos nephrolepis* cuttings in each group of Examples 1-9 was statistically analyzed, and the results are shown in Table 1.

[0067] Table 1. Rooting rate of *Sterculia lanceolata* cuttings in groups 1-9 of Examples 1

[0068]

[0069] Example 10

[0070] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0071] Compared with Example 1, the difference is that the allicin solution is replaced with the commonly used naphthaleneacetic acid solution (500 mg / L), while the other conditions are the same as in Example 1.

[0072] Example 11

[0073] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0074] Compared with Example 10, the difference is that the concentration of naphthaleneacetic acid was adjusted to 1000 mg / L, while the other conditions were the same as in Example 10.

[0075] Example 12

[0076] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0077] Compared with Example 10, the difference is that the concentration of naphthaleneacetic acid was adjusted to 1500 mg / L, while the other conditions were the same as in Example 10.

[0078] Example 13

[0079] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0080] Compared with Example 10, the difference is that the cutting substrate is selected as 10mm peat moss, while the other conditions are the same as in Example 10.

[0081] Example 14

[0082] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0083] Compared with Example 13, the difference is that the concentration of naphthaleneacetic acid was adjusted to 1000 mg / L, while the other conditions were the same as in Example 10.

[0084] Example 15

[0085] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0086] Compared with Example 13, the difference is that the concentration of naphthaleneacetic acid was adjusted to 1500 mg / L, while the other conditions were the same as in Example 10.

[0087] Example 16

[0088] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0089] Compared with Example 10, the difference is that the cutting substrate is selected as river sand (0.5mm) and perlite (2mm) with a volume ratio of 1:2, while other conditions are the same as in Example 10.

[0090] Example 17

[0091] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0092] Compared with Example 16, the difference is that the concentration of naphthaleneacetic acid was adjusted to 1000 mg / L, while the other conditions were the same as in Example 10.

[0093] Example 18

[0094] The rapid asexual propagation method for *Sterculia lanceolata* is as follows:

[0095] Compared with Example 16, the difference is that the concentration of naphthaleneacetic acid was adjusted to 1500 mg / L, while the other conditions were the same as in Example 10.

[0096] Examples 10-18 served as comparative experiments, with the substrate, treatment time, and hormone concentration all repeated from Examples 1-9, except that the growth regulator was replaced with naphthaleneacetic acid (NAA), commonly used in cutting experiments. After three months of asexual propagation using the methods described in Examples 10-18, the rooting rate of *Symplocos nephrolepis* cuttings in each group from Examples 10-18 was counted as a control group, and the results are shown in Table 2.

[0097] Table 2. Rooting rate of *Sterculia lanceolata* cuttings in each control group of Examples 10-19

[0098]

[0099] As can be seen from the data in Tables 1 and 2, this invention significantly improves the rooting rate of *Symplocos nephrolepis* cuttings by controlling the cutting substrate, hormone concentration, hormone type selection, and treatment time allocation. The allicin solution used in this invention, compared to the commonly used naphthaleneacetic acid solution for cuttings, increases the rooting rate of *Symplocos nephrolepis* by 30-60%.

[0100] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A method for propagating *Symplocos septemlobus* by cuttings, characterized in that, Includes the following steps: Cuttings of the narrow-fruited cypress were immersed in an allicin solution and then inserted into a rooting medium for cultivation.

2. The method for propagating *Sterculia lanceolata* by cuttings according to claim 1, characterized in that, The cuttings are new, semi-lignified shoots of 1-2 year old narrow-fruited cypress trees.

3. The method for propagating *Sterculia lanceolata* by cuttings according to claim 2, characterized in that, The cuttings are 5-10cm long, and the top part of the cutting retains one leaf that has been cut in half.

4. The method for propagating *Sterculia lanceolata* by cuttings according to claim 1, characterized in that, The concentration of the allicin solution is 500 mg / L, and the cuttings are immersed in the allicin solution for 1 hour.

5. The method for propagating *Sterculia lanceolata* by cuttings according to claim 1, characterized in that, The culture substrate is vermiculite.

6. The method for propagating *Sterculia lanceolata* by cuttings according to claim 1, characterized in that, The cultivation environment is a shaded greenhouse with a relative humidity of 80±10% and a light transmittance of 60~70%.

7. The method for propagating *Sterculia lanceolata* by cuttings according to claim 1, characterized in that, The cuttings should be taken in mid-to-late June.

8. The method for propagating *Sterculia lanceolata* by cuttings according to claim 1, characterized in that, After the cuttings are inserted into the rooting medium, a sterilization process is also included.

9. The method for propagating *Sterculia lanceolata* by cuttings according to claim 8, characterized in that, The disinfection method used was a carbendazim solution diluted 500 times.

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