Insecticidal and acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide pest and mite killing, and discloses a pest and mite killing composition and application thereof.The pest and mite killing composition comprises an active ingredient A and an active ingredient B. The active ingredient A is a compound shown in the formula I, and the structure of the compound shown in the formula I is shown in the specification. The tetronic acid compound is any one of spiromesifen, spirodiclofen, spirotetramat, spirodiclofen diester, spidoxomat or spiropidion, and the mass ratio of the active component A to the active component B is (1: 48)-(48: 1). The insecticidal and acaricidal composition expands the control spectrum of pests and mites, is safe to target crops, delays the development of drug resistance of pests and mites, and reduces pesticide residues.

Description

Technical Field

[0001] This invention belongs to the field of pesticide insecticide and acaricide technology, and discloses an insecticide and acaricide composition and its application. Background Technology

[0002] Piercing-sucking pests are a dominant group among plant pests, mainly including whiteflies, thrips, mites, and aphids. These pests are small, and early symptoms are often subtle and easily overlooked. However, they are numerous, often clustering on tender branches, leaves, buds, flower buds, and fruits, sucking plant sap and robbing them of nutrients. This directly causes various symptoms, leading to curling of branches, leaves, and flowers; in severe cases, it can even cause the entire plant to wither or die. They also directly or indirectly transmit viral diseases and other pests. Without control measures, this can result in serious losses or even total crop failure. Therefore, mitigating the infestation of crop pests and mites, ensuring the quality and safety of agricultural plants, reducing pesticide costs, and mitigating environmental pollution have become current concerns. Finding safe and effective control methods has thus become one of the main approaches to green and safe production.

[0003] In view of this, the applicant selected compound I and six quaternary keto acid compounds to form a compound, and conducted toxicity and efficacy tests on spider mites and aphids in order to provide a basis for the selection and use of control agents. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides an insecticidal and acaricidal composition and its application. The insecticidal and acaricidal composition of this invention has excellent control effects on pests and mites, expands the control spectrum of pests and mites, is safe for target crops, delays the development of pesticide resistance in pests and mites, and reduces pesticide residues.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: an insecticidal and acaricidal composition, wherein the composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I, and the structure of the compound of formula I is shown below: The component B is a quaternary keto acid compound, which is any one of spirodiclofen, spirodiclofen, spirodiclofen diester, spidoxamat, or spiropidion. The mass ratio of active ingredient A to active ingredient B is 1:48 to 48:1, or any value within the above range.

[0006] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:48 to 45:1, or any value within the above range.

[0007] Furthermore, the active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:25 to 35:1, or any value within the above range.

[0008] The active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:36 to 42:1, or any value within the above range.

[0009] The active ingredient B is spirotetramat, and the mass ratio of the compound of formula I to spirotetramat is 1:36 to 45:1, or any value within the above range.

[0010] The active ingredient B is spirodicarboxylate, and the mass ratio of the compound of formula I to spirodicarboxylate is 1:20 to 40:1, or any value within the above range.

[0011] The active ingredient B is spidoxamat, and the mass ratio of the compound of formula I to spidoxamat is 1:48 to 40:1, or any value within the above range.

[0012] The active ingredient B is spiropidion, and the mass ratio of the compound of formula I to spiropidion is 1:30 to 40:1, or any value within the above range.

[0013] Furthermore, the active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:24 to 30:1, or any value within the above range.

[0014] The active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:18 to 36:1, or any value within the above range.

[0015] The active ingredient B is spirotetramat, and the mass ratio of the compound of formula I to spirotetramat is 1:35 to 35:1, or any value within the above range.

[0016] The active ingredient B is spirodicarboxylate, and the mass ratio of the compound of formula I to spirodicarboxylate is 1:10 to 30:1, or any value within the above range.

[0017] The active ingredient B is spidoxamat, and the mass ratio of the compound of formula I to spidoxamat is 1:40 to 20:1, or any value within the above range.

[0018] The active ingredient B is spiropidion, and the mass ratio of the compound of formula I to spiropidion is 1:20 to 40:1, or any value within the above range.

[0019] The composition of the present invention exhibits a synergistic effect on semi-piercing-sucking pests at any value within the above numerical range.

[0020] Furthermore, the total weight of the insecticidal and acaricidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the composition.

[0021] Furthermore, in addition to the active ingredient, the composition also contains pesticide-permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0022] Furthermore, the composition is prepared into a pesticide-permitted formulation, wherein the formulation is a solid or liquid formulation;

[0023] Furthermore, the solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension concentrate, emulsifiable concentrate, dispersible oil suspension, or water emulsion;

[0024] The present invention also discloses the application of the insecticidal and acaricidal composition described above for the control of piercing-sucking plant pests or mites.

[0025] Furthermore, the pests are hemiptera pests, and the mites are mites from the order Acari.

[0026] Furthermore, the aforementioned hemiptera pests include aphids, scale insects, whiteflies, and leafhoppers: pea aphid (Acrythosiphonpisum), adelges spp. (adelgids), cabbage whitefly (Aleurodes proletella), spiral whitefly (Aleurodicus disperses), woolly whitefly (Aleurothrixus flccosus), white-spotted scale (Aluacaspisspp.), Amrasca bigutella bigutella, leafhopper (Aphrophora spp.), red scale (Aonidiella aurantii), aphid (Aphis spp.), cotton aphid (Aphis gossypii), and apple aphid (Aphis... pomi (apple aphid), Aulacorthitm solani (foxgloveaphid), Bemisia spp. (whitefly), Bemisia argentifolii, Bemisia tabaci (sweetpotato whitefly), Brachycolusnoxius (Russian aphid), Brachycorynclia asparagi (asparagusaphid), Brevennia rehi, Brevicoryne brassicae (cabbage aphid), Ceroplastes spp. (scale insect), Ceroplastes rubens (red wax scale), Chionaspis spp. (scale insect), Chrysomphalus spp. (scale insect), Coccus spp. (scale insect), Dysaphis plantaginea (rosy apple aphid), Empoasca spp. (scale insect).(Leafhopper), Apple cotton aphid (Eriosomalanigerum) (woolly apple aphid), Cottony cushion scale (Icerya purchasi) (cottony cushion scale), Mango leafhopper (Idioscopus nitidulus) (mango leafhopper), Smaller brown planthopper (Laodelphax striatellus) (smaller brown planthopper), Lepidosaphes spp., Macrosiphum spp., Potato aphid (Macrosiphum euphorbiae), English grain aphid (Macrosiphumgranarium), Rose aphid (Macrosiphum rosae), Asterleafhopper (Macrosteles quadrilineatus), Mahanarva frimbiolata, Metopolophium * *dirhodum* (rose grain aphid), *Midis longicornis*, *Myzuspersicae* (green peach aphid), *Nephotettix spp.* (leafhopper), *Nephotettix cinctipes* (green leafhopper), *Nilaparvata lugens* (brown planthopper), *Parlatoria pergandii* (chaff scale), *Parlatoria ziziphi* (ebony scale), *Peregrinus maidis* (corndelphacid), *Philaenus spp.* (blower), *Phylloxera vitifoliae* (grape phylloxera), *Physokermes piceae* (spruce budscale), *Planococcus* (mealyptic mealybug). (Pseudococcus spp.) (meat mealybug), species of the genus Pseudococcus spp.(Mealybug), Pineapple Mealybug (Pscudococcus brcvipcs), Orchard Scale (Quadraspidiotus perniciosus), Aphid Species (Rhapalosiphum spp.), Corn Leaf Aphid (Rhapalosiphum maida), Oatbird-cherry Aphid (Rhapalosiphumpadi), Scale Beetle (Saissetia spp.), Black Scale Beetle (Saissetiaoleae), Gramineous Aphid (Schizaphis graminum), British Wheat Aphid (Sitobion avenge), White-backed Planthopper (Sogatella) *Furcifera* (white-backed planthopper), *Therioaphis* spp. (aphid), *Toumeyella* spp. (scale insect), *Toxoptera* spp. (aphid), *Trialeurodes* spp. (whitefly), *Trialeurodesvaporariorum* (greenhouse whitefly), *Trialeurodesabutiloneus* (bandedwing whitefly), *Unaspis* spp. (scale insect), *Unaspisyanonensis* (arrowhead scale), and *Zulia entreriana*.

[0027] The aforementioned tick-like pests include species of the genus *Acarus* spp. (food mite), *Acarus siro* (grain mite), *Aceria mangiferae* (mango bud mite), species of the genus *Aculops* spp., *Aculops lycopersici* (tomato russet mite), *Aculops pelekasi*, *Aculus pelekassi*, *Aculus schlechtendali* (apple rust mite), *Brevipalpus obovatus* (privet mite), *Brevipalpus phoenicis* (red and black flat mite), species of the genus *Eotetranycus* spp., and *Eotetranychus* (hornbeam leaf mite). *Carpini* (yellow spider mite), *Epitimerus* spp., *Eriophyes* spp., *Metatetranycus* spp., *Oligonychus* spp., *Oligonychus coffee*, *Oligonychus ilicus* (southernred mite), *Panonychus* spp., *Panonychus cirri* (citrus red mite), *Panonychus ulmi* (European red mite), *Phyllocoptruta oleivora* (citrus rustmite), *Polyphagotarsonemunlatus* (broad mite) The following mites are listed: *Bulbmite* (a type of mite), *Sarcoptes scabiei* (a type of scabies mite), *Tegolophus perseaflorae* (a type of avocado gall mite), and *Tetranychus spp.* (a type of spider mite).The following are listed as examples of spider mites: *Tetranychus urticae* (two-spotted spider mite), *Tetranychus cinnabarinus*, *Tetranychus viennensis*, and *Tetranychus truncatus*.

[0028] In particular, the composition of the present invention has excellent control effects on Tetranychus cinnabarinus, Tetranychus two-spotted, Tetranychus truncatus, Tetranychus citrinum, Tetranychus maculata, whiteflies, and aphids.

[0029] To achieve the desired insecticidal effect, the dosage of this composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application location, application method, and formulation used.

[0030] The beneficial effects of this invention are as follows:

[0031] Compared with single agents, the composition of the present invention has a significant synergistic effect on common piercing-sucking pests in agriculture, overcomes and delays drug resistance, expands the control spectrum, and significantly improves the control effect; it reduces labor and pesticide costs, and achieves significant control effect with a single application. Detailed Implementation

[0032] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0033] Formulation preparation example:

[0034] Preparation Example 1: 24% Formula I compound·spirodiclofen suspension (1:5)

[0035] Formula composition: by weight percentage, 4% Formula I compound, 20% spirodiclofen, 0.5% sodium lignosulfonate, 2% Gelbert alcohol polyoxyethylene ether, 3% polyoxyethylene dehydrated sorbitan monooleate, 0.5% sodium polyacrylate, 3% styrene-phenol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.2% sodium sorbate, 0.2% silicone oil, and deionized water to make up the balance.

[0036] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0037] Preparation Example 2: 30% Formula I compound·spirodiclofen water-dispersible granules (3:2)

[0038] Formula composition: by weight percentage, 18% Formula I compound, 12% spirodiclofen, 7% naphthalene sulfonate formaldehyde condensate, 8% sodium polycarboxylate, 2% sodium dodecyl sulfate, 10% corn starch, and kaolin to make up the balance.

[0039] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0040] Preparation Example 3: 33% Formula I compound·spirodiclofen wettable powder (10:1)

[0041] Formula composition: by weight percentage, 30% Formula I compound, 3% spirodiclofen, 10% dispersant NNO, 5% dispersant BX, 6% sodium alkyl polyoxyethylene ether sulfonate, 5% sodium lignosulfonate, 15% silica, and kaolin to make up the balance.

[0042] Preparation method: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a stirring tank. The mixture is then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the present invention.

[0043] Preparation Example 4: 22.5% Formula I compound·spirodiclofen suspension (1:14)

[0044] Formula composition: by weight percentage, 1.5% of Formula I compound, 21% spirodiclofen, 1% naphthalene sulfonate formaldehyde condensate, 4% styrene phenol polyoxyethylene ether phosphate, 2% sodium dodecyl sulfate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance.

[0045] Preparation method: Same as in preparation example 1.

[0046] Preparation Example 5: 6% Formula I compound·spirodiclofen water emulsion (1:3)

[0047] Formula composition: by weight percentage, 1% Formula I compound, 5% spirodiclofen, 12% toluene, 5% alkyl aryl polyoxyethylene polyoxypropylene ether, 10% acetophenone, 10% cyclohexanone, 1% sodium alkyl polyoxyethylene ether sulfonate, 0.15% xanthan gum, 4% ethylene glycol, 0.1% benzoic acid, 0.2% polyoxypropylene polyoxyethylene glycerol ether, and deionized water to make up the balance.

[0048] Preparation method: According to the formulation ratio in the example, the active ingredient, solvent, emulsifier, and cosolvent are added together to dissolve into a uniform oil phase; a portion of water, antifreeze, and other pesticide adjuvants are mixed together to form a uniform aqueous phase; while stirring at high speed in the reactor, the oil phase is added to the aqueous phase, and after stirring evenly, the shearing machine is turned on for high-speed shearing, and the remaining water is added. Shearing is carried out for about half an hour to form an oil-in-water emulsion.

[0049] Preparation Example 6: 10% Formula I compound·spirodiclofen emulsifiable concentrate (9:1)

[0050] Formulation composition: by weight percentage, 9% Formula I compound, 1% spirodiclofen, 10% DMF, 10% styrene-phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 12% propylene carbonate, 10% cyclohexanone, with xylene to make up the balance.

[0051] Preparation method: Dissolve the active ingredient in an organic solvent, then add emulsifiers and other additives, mix and dissolve under stirring to form a homogeneous and transparent liquid, which is the emulsifiable oil.

[0052] Preparation Example 7: 26% Formula I compound·spirotetramat suspension (1:12)

[0053] Formula composition: by weight percentage, 2% Formula I compound, 24% spirotetramat, 3% alkylphenol polyoxyethylene ether phosphate, 1% naphthalene sulfonate formaldehyde condensate, 2% alkyl aryl polyoxyethylene polyoxypropylene ether, 1% sodium lignosulfonate, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.3% xanthan gum, 2% sodium sorbate, 0.5% silicone oil, and deionized water to make up the balance.

[0054] Preparation method: Same as in preparation example 1.

[0055] Preparation Example 8: 33% Formula I compound·spirotetramat water-dispersible granules (5:1)

[0056] Formula composition: by weight percentage, 27.5% Formula I compound, 5.5% spirotetramat, 12% sodium lignosulfonate, 6% sodium polycarboxylate, 2% sodium dodecyl sulfate, 10% silica, 30% corn starch, and kaolin to make up the balance.

[0057] Preparation method: Same as in preparation example 2.

[0058] Preparation Example 9: 20% Formula I compound·spirotetramat dispersible oil suspension (9:1)

[0059] Formula composition: by weight percentage, 18% of Formula I compound, 2% spirotetramat, 10% oleic acid polyoxyethylene ether, 10% castor oil polyoxyethylene ether, 3% calcium dodecylbenzene sulfonate, 0.3% silica, 0.3% organobentonite, 1% naphthalene sulfonate formaldehyde condensate, and soybean oil to make up the balance.

[0060] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0061] Preparation Example 10: 22% Formula I compound·spirodimethyl diester suspension (1:10)

[0062] Formula composition: by weight percentage, 2% Formula I compound, 20% spirodiclofen, 2% isotridecyl alcohol polyoxyethylene ether, 4% alkyl polyoxyethylene ether phosphate, 3% alkyl aryl polyoxyethylene polyoxypropylene ether, 1% sodium lignosulfonate, 5% glycerol, 1% magnesium aluminum silicate, 0.25% xanthan gum, 2% sodium sorbate, 0.5% silicone oil, and deionized water to make up the balance.

[0063] Preparation method: Same as in preparation example 1.

[0064] Preparation Example 11: 32% Formula I compound·spirodimethyl diester water-dispersible granules (3:5)

[0065] Formula composition: by weight percentage, 12% Formula I compound, 20% spirodimethyl diester, 8% naphthalene sulfonate formaldehyde condensate, 5% sodium polycarboxylate, 4% sodium lignosulfonate, 2% sodium dodecyl sulfate, 0.5% organosilicon defoamer, and kaolin to make up the balance.

[0066] Preparation method: Same as in preparation example 2.

[0067] Preparation Example 12: 11% Formula I compound·spirodiclofen diester emulsifiable concentrate (10:1)

[0068] Formulation composition: by weight percentage, 10% Formula I compound, 1% spirodimethyl ester, 12% N,N-dimethylformamide, 25% cyclohexanone, 10% alkylaryl polyoxyethylene ether polyoxypropylene ether, 2% calcium dodecylbenzenesulfonate, and xylene to make up the balance.

[0069] Preparation method: Same as in preparation example 6.

[0070] Preparation Example 13: 26% Formula I compound·spidoxamat suspension (12:1)

[0071] Formulation composition: by weight percentage, 24% Formula I compound, 2% spidoxamat, 2% succinate sulfonate, 12% alkylphenol polyoxyethylene ether, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% calcium dodecylbenzenesulfonate, 1% sodium polycarboxylate, 0.3% silica, 0.3% organobentonite, and methyl oleate to make up the balance;

[0072] Preparation method: Same as in preparation example 1.

[0073] Preparation Example 14: 28% Formula I compound·spidoxamat water-dispersible granules (5:3)

[0074] Formula composition: by weight percentage, 17.5% Formula I compound, 10.5% spidoxamat, 10% naphthalene sulfonate formaldehyde condensate, 3% bleaching powder BX, 5% sodium lignosulfonate, and kaolin to make up the balance.

[0075] Preparation method: Same as in preparation example 2.

[0076] Preparation Example 15: 31.5% wettable powder of formula I compound·spidoxamat (1:8)

[0077] Formula composition: by weight percentage, 3.5% Formula I compound, 28% spidoxamat, 10% sodium lignosulfonate, 4% dispersant BX, 5% dispersant MF, and kaolin to make up the balance.

[0078] Preparation method: Same as in preparation example 3.

[0079] Preparation Example 16: 22% Spiropidion (Formula I) water-dispersible granules (1:10)

[0080] Formula composition: by weight percentage, 2% Formula I compound, 20% spiropidion, 3% sodium alkyl naphthalene sulfonate, 7% naphthalene sulfonate formaldehyde condensate, 10% sodium polycarboxylate, 3% sodium dodecyl sulfate, 10% ammonium sulfate, and starch to make up the balance.

[0081] Preparation method: Same as in preparation example 2.

[0082] Preparation Example 17: 20% Formula I compound spiropidion suspension (1:4)

[0083] Formulation composition: by weight percentage, 4% Formula I compound, 16% spiropidion, 3% isotridecyl alcohol polyoxyethylene ether, 5% tristyrylphenol ethoxylated phosphate, 2% naphthalene sulfonate formaldehyde condensate, 4% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.5% magnesium aluminum silicate, 0.2% xanthan gum, 5% glycerol, 0.2% potassium benzisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance.

[0084] Preparation method: Same as in preparation example 1.

[0085] Preparation Example 18: 27% formula I compound spiropidion wettable powder (8:1)

[0086] Formula composition: by weight percentage, 24% Formula I compound, 3% spiropidion, 10% sodium lignosulfonate, 5% dispersant NNO, 2% sodium dodecyl sulfate, 3% silica, and kaolin to make up the balance.

[0087] Preparation method: Same as in preparation example 3.

[0088] Example 1: Indoor Bioactivity Test for Harmful Mites

[0089] Test targets: Tetranychus cinnabarinus nymphs and adult females;

[0090] Test agents: Spirodiclofen, Spirodiclofen, Spirodiclofen, Spirodiclofen diester, Spidoxamat, Spiropidofen, and the technical grade of compound I;

[0091] Pharmaceutical preparation: Dissolve the above raw materials in a suitable solvent to form a mother liquor, and then dilute it with 0.1% Tween-80 aqueous solution in a proportional manner to form 5 mass concentration gradients.

[0092] Experimental Method: The slide immersion method was used. Double-sided tape was cut into 2cm x 2cm pieces and attached to one end of a glass slide. The tested mites were selected, and using a No. 0 brush, they were gently picked up and their backs were placed face down onto the tape. 30 mites were attached to each slide, and each treatment dose was repeated four times, for a total of 120 mites. The slides with mites were placed in a clean, large rearing dish with a damp cotton ball, covered with a glass plate, and incubated at 25℃ for 2 hours before microscopic examination. If any mites died, live mites were added before further testing. During testing, the mite-attached end of the slide was immersed in the test solution for 5 seconds, then removed. Excess solution around the mites and on the tape was immediately blotted with a small piece of filter paper. Treatments with corresponding organic solvents (without the drug) were used as controls. Slides treated with each concentration of drug were returned to the original rearing dish and incubated at 25℃ for 48 hours. The results were then examined using a binocular dissecting microscope. During the inspection, gently touch the mite with the tip of a brush. If the mite does not move or react, it is recorded as a dead individual.

[0093] Data statistics and analysis:

[0094] Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula.

[0095]

[0096] In the formula:

[0097] P – Mortality rate, expressed as a percentage (%);

[0098] K represents the number of dead insects, in heads;

[0099] N represents the total number of insects treated, in units of heads.

[0100]

[0101] In the formula:

[0102] P1 – Corrected mortality rate, in percentage (%);

[0103] P t —The mortality rate is expressed as a percentage (%).

[0104] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0105] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0106] The LC was determined using the DPS statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0107] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0108]

[0109] In the formula:

[0110] ATI – Actual Measured Toxicity Index of Mixtures;

[0111] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0112] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0113] TTI = TI A ×P A +TIB ×P B

[0114] In the formula:

[0115] TTI – Theoretical Toxicity Index of Mixtures;

[0116] TI A —A. Toxicity index of drug A;

[0117] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0118] TI B —Toxicity index of drug B;

[0119] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0120]

[0121] In the formula:

[0122] CTC – Cotoxicity Coefficient;

[0123] ATI – Actual Measured Toxicity Index of Mixtures;

[0124] TTI – Theoretical Toxicity Index of Mixtures.

[0125] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0126] The indoor activity test results are shown in the table below:

[0127] Table 1. Results of indoor bioactivity assays of compound I and spirodiclofen on Tetranychus cinnabarin nymphs.

[0128]

[0129] Table 1 shows the results of the indoor experiments. The combination of compound I and spirodiclofen at an appropriate mass ratio has a significant synergistic effect on *Tetranychus cinnabarinus*. When the mass ratio of compound I to spirodiclofen is 1:24–30:1, the co-toxicity coefficient against *Tetranychus cinnabarinus* is greater than 120, indicating a synergistic effect. When the mass ratio is 1:12–24:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:6–12:1, the co-toxicity coefficient is greater than 150, showing a significant synergistic effect. Among these, the co-toxicity coefficient is the highest at a mass ratio of 3:2, reaching 188.397.

[0130] Table 2 shows the results of indoor bioactivity assays of compound I and spirodiclofen in combination with Tetranychus cinnabarin nymphs.

[0131]

[0132] Table 2 shows the results of indoor experiments. The combination of compound I and spirodiclofen at appropriate mass ratios has a significant synergistic effect on *Tetranychus cinnabarinus*. When the mass ratio of compound I to spirodiclofen is 1:36–42:1, the co-toxicity coefficient against *Tetranychus cinnabarinus* is greater than 120, indicating a synergistic effect. When the mass ratio is 1:18–36:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:9–18:1, the co-toxicity coefficient is greater than 140, showing a remarkable synergistic effect. Among these, the mass ratio of compound I to spirodiclofenus is 1:3, resulting in the highest co-toxicity coefficient against *Tetranychus cinnabarinus*, at 168.584.

[0133] Table 3. Results of indoor bioactivity assays of compound I in combination with spirotetramat against Tetranychus cinnabarinus nymphs.

[0134]

[0135] Table 3 shows the results of the indoor experiments. The combination of compound I and spirotetramat at an appropriate mass ratio has a significant synergistic effect on *Tetranychus carinata*. When the mass ratio of compound I to spirotetramat is 1:45–35:1, the co-toxicity coefficient against *Tetranychus carinata* is greater than 120, indicating a synergistic effect. When the mass ratio is 1:35–25:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:25–25:1, the co-toxicity coefficient is greater than 140, showing a remarkable synergistic effect. Among these, the mass ratio of compound I to spirotetramat is 1:2, resulting in the highest co-toxicity coefficient against *Tetranychus carinata*, at 199.227.

[0136] Table 4. Results of indoor bioactivity assays of compound I and spirodiester on adult Tetranychus cinnabarinus.

[0137]

[0138] Table 4 shows the results of the indoor experiments. The combination of compound I and spirodiclofen at an appropriate mass ratio has a significant synergistic effect on *Tetranychus cinnabarinus*. When the mass ratio of compound I to spirodiclofen is 1:20–40:1, the co-toxicity coefficient against *Tetranychus cinnabarinus* is greater than 120; when the mass ratio is 1:10–30:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect; when the mass ratio is 1:5–20:1, the co-toxicity coefficient is greater than 150, indicating a remarkable synergistic effect. Among these, the mass ratio of compound I to spirodiclofenus is 5:1, resulting in the highest co-toxicity coefficient against *Tetranychus cinnabarinus*, at 179.023.

[0139] Table 5. Results of indoor bioactivity assays of compound I and Spidoxamat in combination with adult Tetranychus cinnabarinus.

[0140]

[0141] Table 5 shows the results of the indoor experiments. The combination of compound I and Spidoxamat at an appropriate mass ratio has a significant synergistic effect on *Tetranychus carinata*. When the mass ratio of compound I to Spidoxamat is 1:48–40:1, the co-toxicity coefficient against *Tetranychus carinata* is greater than 120, indicating a synergistic effect. When the mass ratio is 1:40–32:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:40–16:1, the co-toxicity coefficient is greater than 140, showing a remarkable synergistic effect. Among these, the co-toxicity coefficient is the highest at a mass ratio of 1:8, reaching 195.345.

[0142] Table 6. Results of indoor bioactivity assays of compound I and Spiropidion against adult Tetranychus cinnabarinus.

[0143]

[0144] Table 6 shows the results of the indoor experiments. The combination of compound I and Spiropidion at an appropriate mass ratio has a significant synergistic effect on *Tetranychus carmine*. When the mass ratio of compound I to Spiropidion is 1:20–40:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect. When the mass ratio is 1:10–32:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:10–16:1, the co-toxicity coefficient is greater than 140, showing a remarkable synergistic effect. Among these, the co-toxicity coefficient is the highest at a mass ratio of 4:1, reaching 176.323.

[0145] Example 2: Indoor bioactivity assay of aphids

[0146] Experimental target: aphids.

[0147] Test agents: Spirodiclofen, Spirodiclofen, Spidoxacarb, Spiropidion, and the technical grade of compound I.

[0148] Drug preparation: Dissolve the above raw materials in a suitable solvent, and then dilute them with 0.1% Tween-80 aqueous solution in equal proportions to form 5 mass concentration gradients.

[0149] Experimental Methods: Indoor bioactivity was determined using the immersion method. Fresh, tender shoots of Sichuan pepper infested with aphids were selected. Adult aphids of similar growth were retained, and excess adult aphids were removed. Thirty test insects were used for each treatment, repeated four times. The insects were immersed in the prepared test solution. A 0.1% Tween-80 aqueous solution was used as a blank control. After 5 seconds, the insects were removed, dried, and placed in test bottles lined with moisturizing filter paper. These bottles were then placed in an artificial climate chamber at (25±1)℃, relative humidity 68%–70%, and a photoperiod L:D = 16h:8h. Aphid mortality was assessed 48 hours after treatment, and the number of dead insects was recorded. A death was indicated by lightly touching the abdomen with the tip of a paintbrush; if the legs did not move, the insect was considered dead. Based on the collected data, the mortality rate and corrected mortality rate were calculated.

[0150] Data statistics and analysis:

[0151] Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula.

[0152]

[0153] In the formula:

[0154] P – Mortality rate, expressed as a percentage (%);

[0155] K represents the number of dead insects, in heads;

[0156] N represents the total number of insects treated, in units of heads.

[0157]

[0158] In the formula:

[0159] P1 – Corrected mortality rate, in percentage (%);

[0160] P t —The mortality rate is expressed as a percentage (%).

[0161] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0162] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0163] The LC was determined using the DPS statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0164] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0165]

[0166] In the formula:

[0167] ATI – Actual Measured Toxicity Index of Mixtures;

[0168] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0169] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0170] TTI = TI A ×P A +TI B ×P B

[0171] In the formula:

[0172] TTI – Theoretical Toxicity Index of Mixtures;

[0173] TI A —A. Toxicity index of drug A;

[0174] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0175] TI B —Toxicity index of drug B;

[0176] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0177]

[0178] In the formula:

[0179] CTC – Cotoxicity Coefficient;

[0180] ATI – Actual Measured Toxicity Index of Mixtures;

[0181] TTI – Theoretical Toxicity Index of Mixtures.

[0182] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0183] The indoor activity test results are shown in the table below:

[0184] Table 7. Results of the indoor bioactivity assay of compound I combined with spirodiclofen against aphids.

[0185]

[0186] Table 7 shows the results of indoor experiments, indicating that combining compound I with spirodiclofen at an appropriate mass ratio has a significant synergistic effect on aphids. When the mass ratio of compound I to spirodiclofen is 1:25–35:1, the co-toxicity coefficient against aphids is greater than 120, demonstrating a synergistic effect; when the mass ratio is 1:15–30:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect; when the mass ratio is 1:7–15:1, the co-toxicity coefficient is greater than 150, also showing a significant synergistic effect; among these, the co-toxicity coefficient is the highest at a mass ratio of 2:1, reaching 204.949.

[0187] Table 8. Results of the indoor bioactivity assay of compound I combined with spirotetramat against aphids.

[0188]

[0189]

[0190] Table 8 shows the results of the indoor experiments. The combination of compound I and spirotetramat at an appropriate mass ratio has a significant synergistic effect on aphids. When the mass ratio of compound I to spirotetramat is 1:36–45:1, the co-toxicity coefficient to aphids is greater than 120, indicating a synergistic effect. When the mass ratio is 1:24–45:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:12–36:1, the co-toxicity coefficient is greater than 140, showing a remarkable synergistic effect. Among these, the co-toxicity coefficient is the highest at a mass ratio of 7:1, reaching 175.807.

[0191] Table 9. Results of indoor bioactivity assays of compound I in combination with spidoxamat against aphids.

[0192]

[0193] Table 9 shows the results of the indoor experiments. The combination of compound I and spikexamat at an appropriate mass ratio has a significant synergistic effect on aphids. When the mass ratio of compound I to spikexamat is 1:40–20:1, the co-toxicity coefficient to aphids is greater than 120, indicating a synergistic effect. When the mass ratio is 1:30–10:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:20–5:1, the co-toxicity coefficient is greater than 140, showing a remarkable synergistic effect. Among these, the co-toxicity coefficient is the highest at a mass ratio of 1:5, reaching 184.247.

[0194] Table 10 Results of indoor bioactivity assays of compound I in combination with spiropidion against aphids

[0195]

[0196]

[0197] Table 10 shows the results of the indoor experiments. The results indicate that compound I and spiropidion, when combined at an appropriate mass ratio, have a significant synergistic effect on aphids. When the mass ratio of compound I to spiropidion is 1:30–40:1, the co-toxicity coefficient to aphids is greater than 120, demonstrating a synergistic effect. When the mass ratio is 1:15–30:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:7–15:1, the co-toxicity coefficient is greater than 150, showing a remarkable synergistic effect. Among these, the co-toxicity coefficient is the highest at a mass ratio of 3:1, reaching 198.449.

[0198] Example 3: Field efficacy test for controlling strawberry spider mites and aphids

[0199] Experimental site: Strawberry garden in Taishang Village, Tongshan District, Xuzhou, Zhejiang Province. The strawberries in the experimental site were grown in greenhouses, and the greenhouse used for the test was a second-generation solar greenhouse.

[0200] Experimental crop: Strawberry (Zhang Ji).

[0201] Experimental targets: spider mites (Tetranychus cinnabarinus, Tetranychus two-spotted), aphids (Peach aphid, Cotton aphid).

[0202] Experimental Methods: Strawberry plants were planted in September 2023 with a row spacing of 20 cm and a plant spacing of 15 cm. Each treatment was replicated four times. All experimental plots were arranged in a randomized block design, with each plot measuring 30 m². 2 Protective rows were set up between the experimental sections. The experiment used a Gongnong-16 backpack electric sprayer for spraying, focusing on spraying both sides of the leaves.

[0203] Investigation methods: Before applying the pesticide, the initial insect population was investigated. 3 and 7 days after application, the number of spider mites and aphids was investigated. 20 strawberry plants were randomly selected from each plot, and 5 leaves were selected from each plant. The number of insects in each treatment was recorded, and the insect population reduction rate and control effect were calculated.

[0204] Methods for calculating drug efficacy:

[0205]

[0206]

[0207] The results of the field efficacy trials are shown in the table below:

[0208] Table 11 Results of efficacy tests of compound compositions for controlling strawberry spider mites

[0209]

[0210]

[0211] Table 11 shows that all compound treatment groups had good control effects against strawberry spider mites. Seven days after application, the compound treatment groups showed better control effects, with control efficacy reaching over 81%, which was higher than the conventional single-agent control.

[0212] Table 12 Results of efficacy tests of compound compositions for controlling strawberry aphids

[0213]

[0214] Table 12 shows that all four compound pesticide combinations exhibited varying degrees of control efficacy against strawberry aphids. The 30% Formula I compound·spiropidion water-dispersible granules (3:2) showed the best control effect against strawberry aphids, with significantly higher control efficacy than other treatments. The control efficacy was 83.89% after 3 days and 95.02% after 7 days. The 27% Formula I compound·spiropidion wettable powder (8:1), 28% Formula I compound·spidoxamat water-dispersible granules (5:3), and 20% Formula I compound·spiropidion oil-dispersible suspension (9:1) treatments showed control efficacy exceeding 73% after 3 days and exceeding 80% after 7 days, demonstrating good control efficacy against strawberry aphids.

[0215] In summary, through indoor toxicity testing and field efficacy trials, it can be seen that the composition of the present invention has a good control effect on mites and hemiptera pests, and is safe for target crops. It has significant control effect and is superior to single agents in delaying the development of resistance and prolonging the duration of effect.

[0216] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. An insecticidal and acaricidal composition, characterized in that, The composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I, and the structure of the compound of formula I is shown below: The component B is a quaternary keto acid compound, which is any one of spirodiclofen, spirodiclofen, spirodiclofen diester, spidoxamat, or spiropidion. The mass ratio of active ingredient A to active ingredient B is 1:48 to 48:

1.

2. The insecticidal and acaricidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:48 to 45:

1.

3. The insecticidal and acaricidal composition according to claim 1, characterized in that, The active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:25 to 35:

1. The active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:36 to 42:1; The active ingredient B is spirotetramat, and the mass ratio of the compound of formula I to spirotetramat is 1:36 to 45:

1. The active ingredient B is spirodimethyl diester, and the mass ratio of the compound of formula I to spirodimethyl diester is 1:20 to 40:

1. The active ingredient B is spidoxamat, and the mass ratio of the compound of formula I to spidoxamat is 1:48 to 40:1; The active ingredient B is spiropidion, and the mass ratio of the compound of formula I to spiropidion is 1:30 to 40:

1.

4. The insecticidal and acaricidal composition according to claim 3, characterized in that, The active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:24 to 30:

1. The active ingredient B is spirodiclofen, and the mass ratio of the compound of formula I to spirodiclofen is 1:18 to 36:

1. The active ingredient B is spirotetramat, and the mass ratio of the compound of formula I to spirotetramat is 1:35 to 35:

1. The active ingredient B is spirodicarboxylate, and the mass ratio of the compound of formula I to spirodicarboxylate is 1:10 to 30:

1. The active ingredient B is spidoxamat, and the mass ratio of the compound of formula I to spidoxamat is 1:40 to 20:1; The active ingredient B is spiropidion, and the mass ratio of the compound of formula I to spiropidion is 1:20 to 40:

1.

5. The insecticidal and acaricidal composition according to claim 1, characterized in that, The total weight of the insecticidal and acaricidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the composition.

6. The insecticidal and acaricidal composition according to claim 1, characterized in that, In addition to the active ingredient, the composition contains pesticide-permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

7. The insecticidal and acaricidal composition according to claim 1, characterized in that, The composition is prepared into a pesticide formulation, which may be a solid or liquid formulation.

8. The insecticidal and acaricidal composition according to claim 7, characterized in that, The solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension, emulsifiable concentrate, dispersible oil suspension, or water emulsion.

9. The insecticidal and acaricidal composition according to any one of claims 1-8 for the control of piercing-sucking pests.

10. The application according to claim 9, characterized in that, The pests mentioned are whiteflies, aphids, or mites.

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