Pig manure rapid fermentation-soil remediation combined type complex microbial inoculant and application thereof

Abstract

The invention relates to a pig manure rapid fermentation-soil remediation combined type complex microbial inoculant and application thereof.The complex microbial inoculant is formed by compounding bacillus subtilis, white rot fungi and trichoderma according to a specific proportion, and the bacillus subtilis strain is derived from an efficient compost isolate separated from composted pig manure compost. The microbial inoculum is prepared by adding the composite protective agent to be mixed with the carrier and performing low-temperature drying. During application, the pig manure is subjected to continuous high-energy quantum treatment, and pathogen inactivation and organic matter degradation are achieved through the synergistic effect of a high-frequency electric field and active particles; and then carrying out solid-liquid separation, inoculating the solid part with a complex microbial inoculant, carrying out short-period aerobic fermentation at 40-80 DEG C, and carrying out low-temperature airflow drying at 80-150 DEG C to prepare the solid organic fertilizer with both fertilizer efficiency and soil biocontrol function. And the liquid part is subjected to bipolar high energy quantum treatment and chemical neutralization to produce reclaimed water. Through systematic combination of high-energy quantum activation and the functional complex microbial inoculant, rapid and efficient fermentation and all-component resource utilization of pig manure are realized.

Description

Technical Field

[0001] This invention relates to the field of microbial preparations and agricultural waste resource utilization technology, and in particular to a compound microbial agent for rapid fermentation of pig manure and soil remediation and its application. Background Technology

[0002] In large-scale pig farming, the large amount of manure generated daily, if not properly treated, can easily cause serious environmental pollution and resource waste. Currently, the commonly used manure treatment methods in the industry, such as open-air composting or conventional fermentation, typically suffer from long processing cycles, large land areas required, and the generation of foul odors and secondary pollution during the process. In particular, traditional methods have low decomposition efficiency for recalcitrant components in pig manure such as lignin and cellulose, resulting in inconsistent quality of the produced organic fertilizer, limited humic acid content, and potential residues of pathogens and harmful substances, thus hindering its efficient resource utilization. With the increasing demands for environmental protection and sustainable development in the livestock industry, the market urgently needs a technology that can achieve rapid, efficient, and clean conversion of pig manure. While existing technologies have attempted to use single microbial agents or simple physical methods for auxiliary treatment, they often suffer from limitations such as poor adaptability of the microbial strains, limited functionality, insufficient stability in complex environments, low synergistic efficiency of physical and biological processes, and difficulty in simultaneously achieving harmless and high-value utilization. Summary of the Invention

[0003] The main objective of this invention is to provide a compound microbial agent for rapid fermentation of pig manure and soil remediation, and its application, so as to achieve the goal of rapid and efficient fermentation of pig manure and full-component resource utilization through the systematic combination of compound microbial agent and high-energy quantum activation process.

[0004] To achieve the above objectives, the present invention provides a compound microbial agent for rapid fermentation of pig manure and soil remediation. The compound microbial agent is composed of Bacillus subtilis, white rot fungi and Trichoderma. The percentage of effective viable bacteria of each strain in the compound microbial group is: Bacillus subtilis 50% to 80%, white rot fungi 10% to 30%, and Trichoderma 10% to 25%.

[0005] Furthermore, the compound microbial agent also includes a carrier and a compound protective agent, wherein the compound protective agent includes one or more of sucrose, trehalose and skim milk powder, and the mass percentage of the compound protective agent is 1% to 8%.

[0006] Furthermore, the carrier is selected from one or more of wheat bran, peat moss, and diatomaceous earth.

[0007] Furthermore, the strain of Bacillus subtilis is derived from a local high-efficiency compost isolate obtained by targeted screening and isolation from well-rotted pig manure compost.

[0008] Furthermore, the compound microbial agent is in the form of powder or granules, with a moisture content ≤10%.

[0009] This invention also provides a method for treating pig manure with a compound microbial agent, comprising:

[0010] Continuous high-energy quantum processing of pig manure;

[0011] The material after high-energy quantum processing is subjected to solid-liquid separation to obtain solid fecal residue and liquid wastewater;

[0012] The compound microbial agent is mixed with the solid fecal residue and then subjected to aerobic fermentation.

[0013] The fermented material is dried by low-temperature airflow to obtain solid bio-organic fertilizer.

[0014] Furthermore, the continuous high-energy quantum processing utilizes the synergistic effect of a high-frequency electric field and active particles.

[0015] Furthermore, the temperature of the aerobic fermentation is 40°C to 80°C, the aerobic fermentation is carried out after inoculation with the compound microbial agent, and the total fermentation treatment time is controlled to be 1 hour to 7 days; the temperature of the low-temperature airflow drying is 80°C to 150°C.

[0016] Furthermore, the method also includes bipolar high-energy quantum treatment of the liquid wastewater and chemical neutralization using an acid-base regulator to obtain reclaimed water.

[0017] Furthermore, the acid-base regulator is selected from one or more of sulfuric acid, hydrochloric acid, sodium hydroxide, sodium carbonate, and lime.

[0018] The compound microbial agent for rapid fermentation of pig manure and soil remediation provided by this invention and its application have the following beneficial effects:

[0019] Compared with existing technologies, this invention, through the synergistic effect of "continuous high-energy quantum physical activation" and "biotransformation with specific compound microbial agents," shortens the traditional composting cycle of tens of days to 1-7 days, significantly improving treatment efficiency and facilitating continuous and large-scale treatment of manure. The high-energy quantum activation pretreatment efficiently inactivates pathogens and effectively removes malodorous gases. Combined with the deep fermentation of the compound microbial agents, the entire treatment process essentially achieves simultaneous removal of odor, pathogens, and harmful substances, with no significant secondary pollution, making it environmentally friendly. The prepared solid bio-organic fertilizer has a high organic matter content and a significantly increased humic acid content, exhibiting superior fertilizer efficiency compared to conventional compost products. Simultaneously, the liquid portion, after treatment, produces reclaimed water, enabling recycling and enhancing the overall resource value of manure. Furthermore, the core Bacillus subtilis in the compound microbial agent is derived from a highly efficient compost isolate, exhibiting strong adaptability to the manure environment; the agent undergoes composite stabilization treatment with protectants and carriers, ensuring its activity and durability during storage and application. This invention integrates pretreatment, fermentation, drying, and water treatment into a single, streamlined system, significantly reducing the floor space required compared to traditional composting and improving space utilization. Furthermore, it can be configured for automated or semi-automated operation, reducing manual labor intensity. The absence of large amounts of additives or commercial microbial agents after pretreatment helps control operating costs and enhances the technology's operability and potential for widespread adoption in actual production. Attached Figure Description

[0020] Figure 1 This is a schematic flowchart of a method for treating pig manure with a compound microbial agent according to an embodiment of the present invention.

[0021] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0023] This invention proposes a compound microbial agent for rapid fermentation of pig manure and soil remediation. The compound microbial agent is composed of Bacillus subtilis, white rot fungi and Trichoderma. The percentage of effective viable bacteria of each strain in the compound microbial group is: Bacillus subtilis 50% to 80%, white rot fungi 10% to 30%, and Trichoderma 10% to 25%.

[0024] The compound microbial agent further includes a carrier and a compound protective agent. The compound protective agent includes one or more of sucrose, trehalose, and skim milk powder, and the mass percentage of the compound protective agent is 1% to 8%. The carrier is selected from one or more of wheat bran, peat moss, and diatomaceous earth. The strain of Bacillus subtilis is derived from a local high-efficiency compost isolate obtained by directional screening from well-rotted pig manure compost. The compound microbial agent is in the form of powder or granules, with a moisture content ≤10%.

[0025] Example 1:

[0026] This invention proposes a compound microbial agent for rapid fermentation of pig manure and soil remediation. This compound microbial agent consists of Bacillus subtilis, white-rot fungi, and Trichoderma, with the following percentages of effective viable bacteria: Bacillus subtilis 50%–80%, white-rot fungi 10%–30%, and Trichoderma 10%–25%. This formulation is designed based on functional complementarity and synergistic effects: Bacillus subtilis, as the dominant bacterium, is responsible for rapidly initiating fermentation, decomposing easily degradable organic matter such as proteins and starches, and regulating the pH of the system to inhibit the growth of harmful bacteria; white-rot fungi, as lignin-degrading bacteria, effectively break down the lignin structure of fibrous bedding materials such as straw in manure by secreting enzyme systems such as lignin peroxidase; Trichoderma not only secretes cellulase to assist in the decomposition of organic matter, but its key role is to inhibit or antagonize various soil-borne pathogenic fungi. The introduction of this strain aims to give the final organic fertilizer product soil biocontrol functions, which can improve the microecological environment when directly applied to the soil. To ensure the activity and stability of the compound microbial agent during processing, storage, and subsequent application, this embodiment employs the following process for formulation preparation: The three functional microorganisms mentioned above are separately subjected to high-density fermentation culture to prepare high-concentration bacterial suspensions. Then, they are compounded according to the stated proportions and uniformly mixed with a sterilized, dried mixing carrier. The mixing carrier is selected from one or more of wheat bran, peat moss, and diatomaceous earth, used to adsorb the microorganisms and provide physical support. During the mixing process, a composite protective agent with a mass percentage of 1%–8% is added simultaneously. This composite protective agent includes sucrose, trehalose, skim milk powder, or combinations thereof, to effectively protect the microorganisms from damage during subsequent drying and storage. After uniform mixing, low-temperature airflow drying technology is used, controlling the inlet air temperature at 40-60℃, drying until the material moisture content is ≤10%, ultimately producing a powdered or granular finished compound microbial agent. This drying condition is mild and can maximize the survival rate of the microorganisms. Preferably, the Bacillus subtilis strain in this embodiment is derived from a local high-efficiency compost isolate obtained through targeted screening from well-rotted pig manure compost. This strain, naturally acclimatized to the composting environment, exhibits strong adaptability and highly efficient organic matter decomposition capabilities in livestock and poultry manure substrates. As the core microbial strain for initiating fermentation, it ensures rapid occupation of its ecological niche and dominant role in subsequent treatment. This embodiment utilizes an optimized combination of specific functional microbial strains, a stabilization process combining a composite protective agent with carrier adsorption, and the selection of locally sourced compost strains with high degradation capabilities to construct a specialized composite microbial agent integrating rapid fermentation and soil remediation potential. This provides a core microbial preparation for the efficient resource utilization and agricultural recycling of pig manure.

[0027] Reference Figure 1 This is a schematic flowchart of a method for treating pig manure using a combined compound microbial agent for rapid fermentation and soil remediation, as proposed in this invention. The method includes the following steps:

[0028] S1, continuous high-energy quantum processing of pig manure;

[0029] S2, the material after high-energy quantum processing is subjected to solid-liquid separation to obtain solid fecal residue and liquid wastewater;

[0030] S3, the compound microbial agent is mixed with the solid fecal residue and aerobic fermentation is carried out;

[0031] S4 involves drying the fermented material using low-temperature airflow to obtain solid bio-organic fertilizer.

[0032] The continuous high-energy quantum processing utilizes the synergistic effect of a high-frequency electric field and active particles.

[0033] The aerobic fermentation temperature is 40℃ to 80℃, and the aerobic fermentation is carried out after inoculation with compound microbial agent. The total fermentation treatment time is controlled to be 1 hour to 7 days. The temperature of the low-temperature airflow drying is 80℃ to 150℃.

[0034] It also includes bipolar high-energy quantum treatment of the liquid wastewater and chemical neutralization using an acid-base regulator to obtain reclaimed water.

[0035] The acid-base regulator is selected from one or more of sulfuric acid, hydrochloric acid, sodium hydroxide, sodium carbonate, and lime.

[0036] Example 2:

[0037] The collected pig manure undergoes continuous high-energy quantum processing. This processing unit generates a high-frequency electric field and activates high-concentration active particles (such as ozone and hydroxyl radicals). Utilizing the synergistic effect of the physical effect of the high-frequency electric field and the strong oxidizing chemical effect of the active particles, pathogenic microorganisms (including bacteria, viruses, and parasite eggs) in the manure are rapidly inactivated, while partially breaking down large organic molecules, improving the accessibility of subsequent biological treatment and laying the foundation for efficient fermentation. The pretreated material undergoes solid-liquid separation (e.g., using screw extrusion or sieving) to obtain a solid portion (manure residue) and a liquid portion (manure water). The solid portion is then subjected to aerobic fermentation to produce fertilizer: the compound microbial agent prepared in Example 1 is inoculated into the solid manure residue, typically at 0.1%-0.5% of the dry weight of the solid portion. Aerobic fermentation is carried out under controlled conditions, maintaining an aerobic environment through forced ventilation or turning, and controlling the temperature between 40°C and 80°C. Under these conditions, the functional microorganisms in the compound microbial agent are rapidly activated and work synergistically, shortening the fermentation cycle to 1 hour to 7 days, achieving rapid maturation. The composted solid material is rapidly dried under a low-temperature airflow of 80℃ to 150℃. This temperature window effectively removes moisture while preserving the active nutrients, humus, and some beneficial microorganisms in the organic fertilizer, ultimately yielding high-quality solid bio-organic fertilizer. The liquid portion is then treated: the liquid obtained from solid-liquid separation undergoes bipolar high-energy quantum treatment, further degrading residual organic matter, killing pathogens, and breaking down colloidal instability through enhanced oxidation. Subsequently, chemical neutralization is performed, using acid-base regulators (such as sulfuric acid, hydrochloric acid, sodium hydroxide, sodium carbonate, or lime, or one or more) to adjust and stabilize the effluent quality, ultimately producing reclaimed water that meets agricultural reuse or specific discharge standards. This reclaimed water can be reused for moisture regulation during the fermentation process or used as a base liquid for further formulation of liquid fertilizer. This embodiment integrates high-energy quantum pretreatment with specific functional compound microbial agents to construct an efficient and short-process pig manure treatment route. It not only enables the solid manure residue to be rapidly transformed into high-quality organic fertilizer with soil remediation potential within hours to days, but also simultaneously completes the purification and resource utilization of the liquid portion, truly achieving the goal of full, rapid, and high-value utilization of pig manure.

[0038] The improvements and advantages of the treatment method described in this invention compared to traditional livestock and poultry manure treatment technologies are analyzed from several key dimensions below, as shown in Table 1:

[0039] Comparison Projects Traditional livestock and poultry manure treatment methods Processing method of the present invention Processing objects It can only process solid sewage. Solid, liquid, and gaseous sewage can all be completely rendered harmless. Secondary pollution The emission of foul-smelling gases can easily cause secondary pollution. A fully enclosed system with no leaks, no smoke, and no odor diffusion. Additives / Expectorants Biological inoculants (cost 70-120 yuan / ton) and bedding material need to be added. No biological agents or excipients need to be added. Removal of harmful substances Difficult to completely remove It can completely remove harmful substances from feces and sewage. Processing cycle 15-30 days (longer at low temperatures) 1 hour to 7 days Area Large composting sites are needed Only one-quarter of the traditional process Operational complexity Requires a large number of personnel or equipment, and involves high labor intensity. It can be completed by only 2-3 people and requires little labor intensity. Product conversion rate The effective conversion rate is low, and it cannot produce liquid organic fertilizer at the same time. 5-7 tons of manure can produce 1 ton of high-quality solid fertilizer + 3-5 tons of liquid fertilizer Organic fertilizer quality Low fertilizer efficiency, requires large-scale spreading, and may affect soil health. High fertility; small amounts can improve the soil environment.

[0040] Table 1

[0041] As can be seen from the comparison in the table above, the compound microbial agent and its application method provided by the present invention, without the need to add exogenous microbial agents and excipients, systematically solves the problems of long treatment cycle, serious secondary pollution, large land area and low product added value in traditional methods through a short-process synergistic process of "physical activation + biological transformation", and achieves the unity of high efficiency, cleanliness and high resource value in pig manure treatment.

[0042] In summary, the compound microbial agent is composed of Bacillus subtilis, white-rot fungi, and Trichoderma in a specific ratio. The Bacillus subtilis strain is derived from a highly efficient compost isolate from well-rotted pig manure compost. The agent is prepared by mixing a compound protectant with a carrier and then drying at low temperature. In application, the pig manure is first subjected to continuous high-energy quantum treatment, utilizing the synergistic effect of a high-frequency electric field and active particles to inactivate pathogens and degrade organic matter. Subsequently, solid-liquid separation occurs. The solid portion is inoculated with the compound microbial agent and undergoes short-cycle aerobic fermentation at 40-80℃, followed by low-temperature airflow drying at 80-150℃ to obtain a solid organic fertilizer with both fertilizing and soil biocontrol functions. The liquid portion undergoes bipolar high-energy quantum treatment and chemical neutralization to produce reclaimed water. This invention achieves rapid and efficient fermentation and full-component resource utilization of pig manure through the systematic combination of high-energy quantum activation and functional compound microbial agents.

[0043] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A compound microbial agent for rapid fermentation of pig manure and combined soil remediation, characterized in that, The compound microbial agent is composed of Bacillus subtilis, white-rot fungi, and Trichoderma. The percentage of effective viable bacteria in each strain of the compound microbial group is: Bacillus subtilis 50%–80%, white-rot fungi 10%–30%, and Trichoderma 10%–25%.

2. The compound microbial agent for rapid fermentation of pig manure and soil remediation as described in claim 1, characterized in that, The compound microbial agent also includes a carrier and a compound protectant. The compound protectant includes one or more of sucrose, trehalose and skim milk powder, and the mass percentage of the compound protectant is 1% to 8%.

3. The compound microbial agent for rapid fermentation of pig manure and soil remediation as described in claim 2, characterized in that, The carrier is selected from one or more of wheat bran, peat moss, and diatomaceous earth.

4. The compound microbial agent for rapid fermentation of pig manure and soil remediation as described in claim 1, characterized in that, The strain of Bacillus subtilis was obtained from a local high-efficiency compost isolate obtained by targeted screening and isolation from well-rotted pig manure compost.

5. The compound microbial agent for rapid fermentation of pig manure and soil remediation as described in claim 1, characterized in that, The compound microbial agent is in the form of powder or granules, with a moisture content ≤10%.

6. The method of applying the compound microbial agent to treat pig manure according to any one of claims 1-5, characterized in that, Includes the following steps: Continuous high-energy quantum processing of pig manure; The material after high-energy quantum processing is subjected to solid-liquid separation to obtain solid fecal residue and liquid wastewater; The compound microbial agent is mixed with the solid fecal residue and then subjected to aerobic fermentation. The fermented material is dried by low-temperature airflow to obtain solid bio-organic fertilizer.

7. The method for treating pig manure with the compound microbial agent according to claim 6, characterized in that, The continuous high-energy quantum processing utilizes the synergistic effect of a high-frequency electric field and active particles.

8. The method for treating pig manure with the compound microbial agent according to claim 6, characterized in that, The aerobic fermentation temperature is 40℃ to 80℃, and the aerobic fermentation is carried out after inoculation with compound microbial agent. The total fermentation treatment time is controlled to be 1 hour to 7 days. The temperature of the low-temperature airflow drying is 80℃ to 150℃.

9. The method for treating pig manure with the compound microbial agent according to claim 6, characterized in that, It also includes bipolar high-energy quantum treatment of the liquid wastewater and chemical neutralization using an acid-base regulator to obtain reclaimed water.

10. The method for treating pig manure with the compound microbial agent according to claim 9, characterized in that, The acid-base regulator is selected from one or more of sulfuric acid, hydrochloric acid, sodium hydroxide, sodium carbonate, and lime.

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