A composition with whitening and anti-aging effects and application thereof

Abstract

The present application relates to a kind of composition with whitening and anti-aging and its application, belong to the field of cosmetics.The composition with whitening and anti-aging of the present application includes the following mass parts of components: tetrahydro magnolia alcohol: 0.1-10 parts;Phytol: 1-5 parts;Resveratrol: 0.1-5 parts.The composition with whitening and anti-aging of the present application has significant inhibitory effect on tyrosinase and elastase, and has excellent whitening, anti-aging efficacy.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more particularly to a composition with whitening and anti-aging properties and its application. Background Technology

[0002] With the development of the social economy and the increasing attention consumers pay to their personal image, cosmetics have evolved from basic cleansing and moisturizing functions to include specific effects such as whitening and anti-aging. Skin problems such as pigmentation, dullness, wrinkles, and sagging are mainly induced by factors such as ultraviolet radiation, environmental pollution, and natural aging. The core biological processes involve the excessive synthesis and deposition of melanin, as well as the degradation and loss of the extracellular matrix in the dermis (especially collagen and elastin).

[0003] In the current field of skin-whitening cosmetics, commonly used active ingredients such as arbutin, vitamin C and its derivatives, and kojic acid primarily reduce melanin production by inhibiting tyrosinase activity. However, some traditional skin-whitening ingredients suffer from poor stability (e.g., vitamin C is easily oxidized), strong skin irritation, or are subject to concentration restrictions under regulations, affecting their full efficacy and universal applicability. On the other hand, in the field of anti-aging cosmetics, ingredients such as retinol, peptides, and pro-xylane are widely researched and applied, aiming to stimulate collagen regeneration or inhibit matrix metalloproteinases (MMPs). However, similarly, some highly effective anti-aging ingredients, such as retinol, have drawbacks such as photosensitivity and high irritation, requiring specific conditions for use, thus limiting the user experience.

[0004] In recent years, the market demand for cosmetics with multiple functions (such as simultaneous whitening and anti-aging) that are also gentle and safe has become increasingly strong. However, simply physically mixing commercially available whitening and anti-aging ingredients often fails to achieve synergistic effects and may even affect the safety and efficacy of the final product due to formulation compatibility or irritation issues. Therefore, developing a novel, gentle, and safe composite active composition that achieves synergistic effects through the scientific formulation of specific components, simultaneously targeting key points of melanin production and skin matrix degradation, has become an important research direction in the field of cosmetic technology. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a composition with whitening and anti-aging properties and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] In a first aspect, the present invention provides a composition having whitening and anti-aging properties, comprising the following components in parts by weight:

[0008] Tetrahydromagnoliol: 0.1-10 parts;

[0009] Phytosterol: 10-15 parts;

[0010] Resveratrol: 0.05-20 parts.

[0011] The present invention obtains a whitening and anti-aging composition by selecting a specific range of component ratios. This composition has a significant inhibitory effect on tyrosinase and elastase, and has excellent whitening and anti-aging effects.

[0012] Preferably, the mass ratio of tetrahydromagnoliol to resveratrol is 1:(0.5-5).

[0013] More preferably, the composition comprises the following components in parts by weight:

[0014] Tetrahydromagnoliol: 0.7 parts;

[0015] Phytosterol: 13 parts;

[0016] Resveratrol: 2.8 parts.

[0017] The present invention, by selecting specific mass ratios of components, produces a composition with whitening and anti-aging properties that exhibits a more significant inhibitory effect on tyrosinase and elastase, resulting in superior whitening and anti-aging effects.

[0018] In a second aspect, the present invention provides the use of the composition having whitening and anti-aging effects as described in the first aspect in the preparation of cosmetics having whitening and anti-aging effects.

[0019] Preferably, the dosage form of the cosmetic is any one of cream, lotion, spray, serum, or mask.

[0020] Thirdly, the present invention provides a serum with whitening and anti-aging effects, the serum comprising the composition with whitening and anti-aging effects described in the first aspect.

[0021] Preferably, the essence further includes skin conditioning agents, thickeners, preservatives, and deionized water.

[0022] More preferably, the skin conditioning agent includes at least one of glycerin, sodium hyaluronate, panthenol, and allantoin.

[0023] More preferably, the thickener includes at least one of xanthan gum, sclerotinia gum, and carbomer.

[0024] More preferably, the preservative includes at least one of phenoxyethanol, parabens, and phenoxyethanol.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] 1. Synergistic Effect, Multi-dimensional Targeting: The core beneficial effect of this invention lies in the fact that the three specific active ingredients selected—tetrahydromagnoliol, phytol, and resveratrol—are not simply functionally additive, but rather produce a significant synergistic effect within the specific ratio range disclosed in this invention. This composition constructs a multi-pathway, multi-target skin repair network. In the whitening pathway, the components work synergistically to more comprehensively and efficiently inhibit tyrosinase activity, powerfully blocking melanin synthesis from the source, achieving skin brightening and fading of dark spots. In the anti-aging pathway, the composition works synergistically to protect the dermal structure, particularly by synergistically inhibiting the activity of key degrading enzymes such as elastase. The addition of phytol provides a good lipid compatibility environment for the system, contributing to the stability and delivery of the active ingredients.

[0027] 2. Complementary Mechanisms, Stable Penetration: The three components form a synergistic system under specific ratios. Tetrahydromagnoliol and resveratrol play a key role in inhibiting tyrosinase and elastase, and their mechanisms may be complementary. The addition of phytol not only leverages its inherent skin barrier repair and antioxidant activity, but more importantly, as an excellent lipid-soluble carrier, it can form a stable synergistic system with tetrahydromagnoliol and resveratrol, significantly improving the physicochemical compatibility and stability of the composition, and promoting the transdermal penetration of active ingredients. This ensures that the core active ingredients are delivered more effectively to the target site, fully translating the high in vitro validated activity into observable skin surface effects in humans.

[0028] 3. Gentle and Safe, with Balanced Efficacy: Through scientific ingredient formulation, this composition achieves high efficacy while significantly optimizing the gentleness and safety of the formula. The various ingredients work synergistically to exert their whitening and anti-aging effects, maintaining a balance and reducing the potential irritation risks associated with any single high-concentration ingredient. This allows cosmetics made from this composition to be suitable for a wider range of skin types, ultimately providing users with a high-quality cosmetic solution that combines significant whitening and anti-aging effects with excellent safety. Detailed Implementation

[0029] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] The raw materials used in this invention and their sources are as follows:

[0031] Tetrahydromusanol: Purchased from Shanghai Keqin Technology Co., Ltd., trade name: Anallerg®-Mulan;

[0032] Resveratrol: Purchased from DSM Nutritional Products Ltd., brand name: REGU®-FADE;

[0033] Phytol: Purchased from BASF (China) Co., Ltd., trade name: Phytol;

[0034] All other raw materials, reagents, and experimental equipment were commercially available.

[0035] Preparation of compositions with whitening and anti-aging properties

[0036] Composition 1

[0037] Composed of the following components by mass:

[0038] Tetrahydromagnoliol: 0.7 parts;

[0039] Phytosterol: 13 parts;

[0040] Resveratrol: 2.8 parts;

[0041] Preparation method: Mix the above-mentioned components by weight evenly, seal and store in the dark.

[0042] Composition 2

[0043] Composed of the following components by mass:

[0044] Tetrahydromagnoliol: 0.1 part;

[0045] Phytosterol: 10 parts;

[0046] Resveratrol: 0.05 parts;

[0047] The preparation method is the same as that of composition 1.

[0048] Composition 3

[0049] Composed of the following components by mass:

[0050] Tetrahydromagnoliol: 10 parts;

[0051] Phytosterol: 15 parts;

[0052] Resveratrol: 20 parts;

[0053] The preparation method is the same as that of composition 1.

[0054] Composition 4

[0055] Composed of the following components by mass:

[0056] Tetrahydromagnoliol: 0.7 parts;

[0057] Phytosterol: 13 parts;

[0058] Resveratrol: 0.35 parts;

[0059] The preparation method is the same as that of composition 1.

[0060] Composition 5

[0061] Composed of the following components by mass:

[0062] Tetrahydromagnoliol: 0.7 parts;

[0063] Phytosterol: 13 parts;

[0064] Resveratrol: 0.7 parts;

[0065] The preparation method is the same as that of composition 1.

[0066] Composition ①

[0067] Unlike composition 1, it lacks tetrahydromagnoliol, and the missing mass fraction is made up with resveratrol. The other components, their mass fractions, and preparation methods are the same as those in composition 1.

[0068] Composition ②

[0069] Unlike composition 1, resveratrol is missing, and the missing mass fraction is made up with tetrahydromagnoliol. The other components, their mass fractions, and preparation methods are the same as those in composition 1.

[0070] Composition ③

[0071] Unlike composition 1, this composition lacks phytol. The missing mass fraction is made up by tetrahydromagnoliol and resveratrol in a mass ratio of 0.7:2.8. The remaining components, their mass fractions, and preparation methods are the same as those in composition 1.

[0072] Composition ④

[0073] Unlike composition 1, it lacks resveratrol and tetrahydromagnoliol, and the missing mass fractions are made up with phytol. The remaining components, mass fractions of the components, and preparation methods are the same as those of composition 1.

[0074] Composition ⑤

[0075] Unlike composition 1, resveratrol is replaced with an equal mass of oxidized resveratrol, while the remaining components, mass fractions of components, and preparation methods are the same as in composition 1.

[0076] Composition ⑥

[0077] Composed of the following components by mass

[0078] Tetrahydromagnoliol: 2.8 parts;

[0079] Phytosterol: 3 parts;

[0080] Resveratrol: 0.7 parts;

[0081] The preparation method is the same as that of composition 1.

[0082] Composition ⑦

[0083] Composed of the following components by mass

[0084] Tetrahydromagnoliol: 3 parts;

[0085] Phytosterol: 0.7 parts;

[0086] Resveratrol: 12 parts;

[0087] The preparation method is the same as that of composition 1.

[0088] Preparation of serums with whitening and anti-aging effects

[0089] Serum 1

[0090] Composed of the following components by mass percentage:

[0091] Composition 1: 1 wt%

[0092] Skin conditioning agent: 5 wt%;

[0093] Thickener: 0.5 wt%;

[0094] Preservative: 0.1 wt%;

[0095] Deionized water replenished to 100%;

[0096] The skin conditioning agent is glycerin and panthenol in a 1:1 mass ratio; the thickener is xanthan gum; and the preservative is succinate.

[0097] Preparation method:

[0098] A1: Mix 1 / 2 deionized water with the skin conditioning agent and the composition evenly to obtain mixture A;

[0099] A2: Heat 1 / 3 of the deionized water to 60°C, then add the thickener and stir until well mixed to obtain mixture B;

[0100] A3: Mix mixture A, mixture B cooled to 30°C, the remaining deionized water, and the preservative evenly to obtain the essence.

[0101] Serum 2

[0102] Unlike serum 1, composition 2 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0103] Serum 3

[0104] Unlike serum 1, composition 3 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0105] Serum 4

[0106] Unlike serum 1, composition 4 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0107] Serum 5

[0108] Unlike serum 1, composition 5 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those in serum 1.

[0109] Serum 6

[0110] Composed of the following components by mass percentage:

[0111] Composition 1: 1.5 wt%

[0112] Skin conditioning agent: 5 wt%;

[0113] Thickener: 0.5 wt%;

[0114] Preservative: 0.1 wt%;

[0115] Deionized water replenished to 100%;

[0116] The skin conditioning agent is glycerin and panthenol in a 1:1 mass ratio; the thickener is xanthan gum; the preservative is succinate; the preparation method is the same as that of serum 1.

[0117] Serum 7

[0118] Composed of the following components by mass percentage:

[0119] Composition 1: 0.1 wt%

[0120] Skin conditioning agent: 5 wt%;

[0121] Thickener: 0.5 wt%;

[0122] Preservative: 0.1 wt%;

[0123] Deionized water replenished to 100%;

[0124] The skin conditioning agent is glycerin and panthenol in a 1:1 mass ratio; the thickener is xanthan gum; the preservative is succinate; the preparation method is the same as that of serum 1.

[0125] Serum ①

[0126] Unlike serum 1, composition 1 is replaced by composition ① at the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those in serum 1.

[0127] Serum ②

[0128] Unlike serum 1, composition 2 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0129] Serum ③

[0130] Unlike serum 1, composition 3 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0131] Essence ④

[0132] Unlike serum 1, composition 4 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0133] Essence ⑤

[0134] Unlike serum 1, composition 5 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those in serum 1.

[0135] Essence ⑥

[0136] Unlike serum 1, composition 6 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0137] Essence ⑦

[0138] Unlike serum 1, composition 7 is used to replace composition 1 by the same mass percentage, while the remaining components, component mass percentages, and preparation methods are the same as those of serum 1.

[0139] Blank Essence

[0140] Composed of the following components by mass percentage:

[0141] Skin conditioning agent: 5 wt%;

[0142] Thickener: 0.5 wt%;

[0143] Preservative: 0.1 wt%;

[0144] Deionized water replenished to 100%;

[0145] The skin conditioning agent is glycerin and panthenol in a 1:1 mass ratio; the thickener is xanthan gum; and the preservative is succinate.

[0146] Preparation method:

[0147] A1: Mix 1 / 2 deionized water with the skin conditioning agent until homogeneous to obtain mixture A;

[0148] A2: Heat 1 / 3 of the deionized water to 60°C, then add the thickener and stir until well mixed to obtain mixture B;

[0149] A3: Mix mixture A, mixture B cooled to 30°C, the remaining deionized water, and the preservative evenly to obtain the essence.

[0150] Efficacy verification

[0151] Experiment 1: Inhibitory effect of the composition on tyrosinase

[0152] Sample solutions: Compositions 1-5 and compositions ①-⑦ are prepared as sample solutions, wherein the concentration of each composition is 30 μg / mL.

[0153] Test method:

[0154] Prepare a 0.5 mg / mL L-tyrosine solution and a 100 μg / mL tyrosinase solution using phosphate buffer (pH 6.8) as the solvent. Mix the tyrosine solution, tyrosinase solution, and sample solution thoroughly. Set up a blank control group. Incubate at 37°C for 30 min, and measure the absorbance at 475 nm. Calculate the inhibition rate of tyrosinase activity by the sample using the following formula:

[0155]

[0156] Sample group: 100 μL tyrosine solution + 50 μL sample solution + 50 μL tyrosinase solution;

[0157] Sample blank group: 100 μL tyrosine solution + 50 μL sample solution + 50 μL buffer solution;

[0158] Control group: 100 μL tyrosine solution + 50 μL tyrosinase + 50 μL buffer solution;

[0159] Control group: 100 μL tyrosine solution + 100 μL buffer solution;

[0160] Each group was measured an average of 3 times, and the average value was taken to calculate the tyrosinase inhibition rate. The results are shown in Table 3.

[0161] The measurement results are shown in Table 1.

[0162] Table 1. Inhibitory effect of the composition on tyrosinase

[0163] Group Tyrosinase inhibition rate (%) Composition 1 48.5 Composition 2 32.3 Composition 3 42.1 Composition 4 34.7 Composition 5 35.9 Composition ① 8.2 Composition ② 12.7 Composition ③ 15.4 Composition ④ 3.6 Composition ⑤ 28.1 Composition ⑥ 26.9 Composition ⑦ 20.5

[0164] Based on the data from Test Example 1, the three raw materials described in this invention (tetrahydromagnoliol, phytol, and resveratrol) exhibit a significant synergistic effect, and their combined use significantly inhibits tyrosinase activity. Specifically, the optimal ratio of Composition 1 (0.7:13:2.8) at a concentration of 30 μg / mL achieved an inhibition rate of 48.5% against tyrosinase, which is far beyond the simple summation of the effects of a single component. This is attributed to tetrahydromagnoliol acting as the core inhibitor, complementing resveratrol in the inhibitory pathway, while phytol acts as a key stabilizer and permeation enhancer, with the three components jointly constructing a highly efficient action system. The data strongly support this mechanism: when the core inhibitor tetrahydromagnoliol (Composition ①) is missing from the system, the inhibition rate drops sharply to 8.2%; when the auxiliary inhibitor resveratrol (Composition ②) is missing, the inhibition rate is only 12.7%; and especially when phytol (Composition ③) is missing as a carrier, the inhibition rate is only 15.4%, which confirms the indispensable auxiliary role of phytol in maintaining the activity and delivery efficiency of the entire system. Conversely, composition ④, containing only phytol, showed an inhibition rate as low as 3.6%, directly demonstrating that a simple carrier cannot produce a significant effect when the core active ingredient is missing. Furthermore, changes in the ratio beyond the scope of this invention (such as compositions ⑥ and ⑦) resulted in a significant decrease in the inhibition rate (26.9% and 20.5%, respectively), which conversely demonstrates the necessity of the specific ratio for achieving the synergistic effect of "1+1+1>3". Therefore, only under the specific ratio defined in this invention can the three components perform their respective functions and work synergistically to achieve highly efficient inhibition of tyrosinase activity.

[0165] Experiment 2: Inhibition of elastase by the composition

[0166] This experiment used porcine pancreatic elastase as the test subject and N-succinyl-alanine-alanine-p-nitroaniline (AAAPVN, commercially available) as the substrate. Porcine pancreatic elastase can hydrolyze AAAPVN, and its hydrolysis products can cause an increase in absorbance at a wavelength of 420 nm. The absorbance was measured using a microplate reader to evaluate the firming and anti-wrinkle effects of the examples and comparative examples.

[0167] Test substances: Compositions 1-5, Compositions ①-⑦.

[0168] 1. Solution preparation

[0169] (1) Prepare Tris-HCl buffer (0.1M pH=7.5): Weigh 2.42g Tris into a beaker, add 200mL of ultrapure water, dissolve completely, and then adjust the pH to 7.5 with concentrated HCl.

[0170] (2) Test sample: Take the above test substances, dissolve the test substances in Tris-HCl buffer, prepare a test substance solution with a concentration of 1wt%, and set it as the test sample.

[0171] (3) Prepare substrate solution AAAPVN (2mM): Weigh 4.51mg N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 5mL Tris-HCl buffer.

[0172] (4) Prepare porcine pancreatic elastase solution (0.171 U / mL): Dissolve 280 μL of porcine pancreatic elastase stock solution in 10 mL of Tris-HCl buffer.

[0173] 2. Grouping and Sampling

[0174] The experiment was divided into three groups: the test sample group, the blank control group, and the model control group. Each group had three replicates at the same concentration. The amounts of each solution added are shown in Table 2.

[0175] Table 2 Experimental Groups

[0176] Group Sample addition amount (μL) Porcine pancreatic elastase (μL) AAAPVN (μL) Tris-HCl buffer (μL) Model control group 0 25 50 25 Blank control group 0 0 50 50 Sample group to be tested 25 25 50 0

[0177] 3. Measurement Method

[0178] The reaction was allowed to proceed at room temperature for 15 minutes, and the absorbance was measured at 420 nm using an ELISA reader.

[0179] 4. Calculation formula

[0180] Inhibition rate (%) = [1 - (A1 - A0) / (A2 - A0)] × 100%

[0181] Where: A0 - the average absorbance of the blank control wells;

[0182] A1 - The average absorbance of the sample well;

[0183] The average absorbance of the A2 model control well.

[0184] 5. Test Results

[0185] Table 3 Results of porcine pancreatic elastase inhibition rate

[0186] Sample Name elastase inhibition rate / % Composition 1 52.4 Composition 2 38.6 Composition 3 40.2 Composition 4 45.8 Composition 5 47.3 Composition ① 15.8 Composition ② 20.1 Composition ③ 23.5 Composition ④ 5.3 Composition ⑤ 32.9 Composition ⑥ 31.7 Composition ⑦ 28.4

[0187] Based on the data from Test Example 2, the anti-aging efficacy of the compositions of the present invention also confirms their synergistic design achieved through specific formulation ratios. Composition 1, with its preferred formulation, exhibited an inhibition rate of 52.4% against elastase, significantly higher than other examples (38.6%-47.3%) and all comparative examples. Specifically, when the formulation ratios were not optimal (compositions 2-5), the inhibition rates fluctuated between 38.6% and 47.3%, indicating that this range was generally effective. However, the absence of any core component led to a sharp decline in efficacy: the inhibition rate was only 15.8% when tetrahydromagnoliol (composition ①) was absent; 20.1% when resveratrol (composition ②) was absent; and 23.5% when phytol (composition ③) was absent. Composition ④, containing only phytol, showed an inhibition rate as low as 5.3%, directly demonstrating the weak effect of a single component. Furthermore, composition ⑤, which replaced resveratrol with the structural analog oxidized resveratrol, also showed a significantly lower inhibition rate (32.9%) than composition 1, indicating the specificity of the components. When the formulation deviates completely from the scope of this invention (e.g., compositions ⑥ and ⑦), the inhibition rates (31.7% and 28.4%) are also significantly lower than the preferred formulations. These data collectively demonstrate that tetrahydromagnoliol, phytol, and resveratrol must coexist in the specific formulations described in this invention to achieve efficient inhibition of elastase. The absence of any one of them or improper formulation will lead to the loss of synergistic effect. This, together with the whitening efficacy data of Test Example 1, provides strong support for the "multi-dimensional targeting and synergistic enhancement" technical effect of this invention.

[0188] Test 3: Safety Test

[0189] Using the 2015 "Cosmetic Safety Technical Specifications" as a reference standard, the cosmetic irritation of serums 1-7, serums ①-⑦, and blank serums was evaluated. The test method was a skin patch test, and 30 people aged 16-65 years were randomly distributed to participate in the test.

[0190] Test substances: serums 1-7, serums ①-⑦, and blank serum.

[0191] Test Method: An equal number of test areas as the amount of test substance were marked on the subject's back. The test substance was placed in a patch applicator (0.020-0.025g). The patch applicator containing the test substance was then covered with non-irritating adhesive tape on the subject's back. One test substance was assigned to each test area. The patch was gently pressed with the palm of the hand to ensure even adhesion to the skin surface. The application was left for 24 hours. The patch applicator was removed 30 minutes later, and the skin reaction was observed after the pressure marks disappeared. If the result was negative, observations were repeated at 24 and 48 hours after the patch test.

[0192] Evaluation criteria:

[0193] Grade 0: Negative reaction;

[0194] Grade 1: Suspicious reaction, with only slight erythema;

[0195] Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules;

[0196] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area;

[0197] Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0198] Test results: All subjects had negative skin reactions.

[0199] The above test results show that the essence provided by the present invention is gentle and non-irritating to the skin and is safe to use.

[0200] Experiment 4: Human Efficacy Test

[0201] Subjects aged 40-55 with dull facial skin, fine lines around the eyes, and lack of elasticity were randomly divided into groups of 10. Each group used serums 1-7, 1-7, and a blank serum for 4 weeks. The whitening, spot-fading, anti-wrinkle, and firming effects were objectively evaluated using a multifunctional skin tester, a skin redness and melanin tester, and a skin elasticity tester.

[0202] The test subjects used the aforementioned serum after cleansing their faces in the morning and evening for four consecutive weeks. Data was collected from the test subjects' faces using a skin color difference test probe and a multifunctional skin testing system (DermaLab® Combo, Cortex, Denmark), a skin red and melanin tester and test probe (DermaLab® Combo, Cortex, Denmark), and a skin elasticity tester (DermaLab® Combo, Cortex, Denmark) before use (week 0), after week 2, and after week 4. Changes in skin whiteness (brightness) / L, skin melanin content / MI, skin elasticity R0 value, skin elasticity R2 value, and skin firmness F4 value were calculated before and after the use of the cosmetics.

[0203] in:

[0204] The L value represents the white balance of L*; the larger the value, the more the color leans towards white.

[0205] A higher MI measurement value indicates a higher melanin content in the skin;

[0206] The R0 value represents the firmness of the skin; the smaller the R0 value, the firmer the skin.

[0207] R2 value characterizes the viscoelasticity of the skin. The closer the R2 value is to 1 (100%), the greater the elasticity of the curve and the better the skin elasticity.

[0208] The F4 value is the area within the envelope curve formed during the application and removal of negative pressure; the smaller the value, the firmer the skin.

[0209] Change rate / % = (Measurement value in week 4 - Measurement value in week 0) / Measurement value in week 0 × 100%

[0210] The average of the test results for each group was taken, and the specific results are shown in Table 4.

[0211] Table 4 Results of Human Efficacy Tests

[0212]

[0213] This trial, conducted over a four-week randomized controlled trial on human subjects, objectively quantified the product's ultimate efficacy. Key data showed that after four weeks of using the optimized formula serum, subjects experienced an 8.03% increase in skin brightness (L value), a 14.72% decrease in melanin content (MI index), a 12.39% increase in skin elasticity (R2 value), and significant improvements in firmness (R0 and F4 values) (reduced by 20.26% and 30.57%, respectively). In contrast, the blank control group showed no significant changes in any of its indicators. These human observation data, combined with the aforementioned in vitro mechanisms, form a complete closed loop: the increased skin brightness and decreased melanin content directly confirm that the mechanism described in Test Example 1—"inhibiting tyrosinase activity and reducing melanin synthesis"—achieved a definite whitening effect on real human skin; while the significant improvement in multiple parameters of skin elasticity and firmness confirms that the anti-aging mechanism of "inhibiting elastase and protecting the dermal matrix" in Test Example 2 has successfully translated into visible signs of skin rejuvenation. The test ultimately verified at the clinical level that the composition of the present invention works synergistically through dual pathways to achieve safe and effective comprehensive whitening and anti-aging effects.

[0214] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition with whitening and anti-aging properties, characterized in that, The composition comprises the following components in parts by weight: Tetrahydromagnoliol: 0.1-10 parts; Phytosterol: 10-15 parts; Resveratrol: 0.05-20 parts.

2. The composition according to claim 1, characterized in that, The mass ratio of tetrahydromagnoliol to resveratrol is 1:(0.5-5).

3. The composition according to claim 2, characterized in that, The composition comprises the following components in parts by weight: Tetrahydromagnoliol: 0.7 parts; Phytosterol: 13 parts; Resveratrol: 2.8 parts.

4. The use of the composition according to any one of claims 1-3 in the preparation of cosmetics with whitening and anti-aging effects.

5. The application as described in claim 4, characterized in that, The cosmetic product can be any one of the following forms: cream, lotion, spray, serum, or mask.

6. A serum with whitening and anti-aging effects, characterized in that, The serum comprises the composition according to any one of claims 1-3.

7. The essence as described in claim 6, characterized in that, The serum also includes skin conditioning agents, thickeners, preservatives, and deionized water.

8. The essence as described in claim 7, characterized in that, The skin conditioning agent includes at least one of glycerin, sodium hyaluronate, panthenol, and allantoin.

9. The essence as described in claim 7, characterized in that, The thickener includes at least one of xanthan gum, sclerotinia gum, and carbomer.

10. The essence as described in claim 7, characterized in that, The preservatives include at least one of phenoxyethanol, parabens, and phenoxyethanol.

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