Traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea of calves and lambs and preparation method of traditional Chinese medicine preparation

Abstract

The invention provides a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea of calves and lambs and a preparation method thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The traditional Chinese medicine preparation is prepared from the following traditional Chinese medicinal materials in parts by weight: 1 part of pomegranate bark, 1 part of pomegranate seeds, 3 parts of white halloysite, 3 parts of red halloysite, 3 parts of limonite, 1 part of montmorillonite, 1 part of cuttlebone, 2 parts of liquorice, 3 parts of dried ginger and 2 parts of dark malt. The traditional Chinese medicine preparation is prepared by adopting different superfine grinding processes according to the materials of the medicinal materials, the traditional Chinese medicine preparation is convenient to take, and the superfine particle size can effectively improve the absorption and utilization rate of the body to the medicine and reduce the dosage. The cure rate of the traditional Chinese medicine preparation for the rotavirus diarrhea of the young livestock can reach 90%, and the negative conversion rate of the rotavirus can reach 100%. The traditional Chinese medicine preparation has an exact curative effect on the rotavirus diarrhea of the young livestock, contains safe and mild components, has no toxic or side effect on organisms, and can be widely popularized and applied.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese veterinary medicine technology, specifically to a traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs, and its preparation method. Background Technology

[0002] Diarrhea in young livestock is one of the most common and extremely harmful problems in animal husbandry, sometimes even devastating. According to authoritative statistics, the morbidity rate of diarrhea in calves under 3 weeks of age reaches over 28%, with a mortality rate exceeding 20%; the morbidity rate in lambs under 3 weeks of age can reach over 30%, with a mortality rate reaching 20%; and the mortality rate in suckling pigs is close to 20%, with diarrhea accounting for over 70% of these deaths. Furthermore, diarrhea disrupts the balance of microorganisms in young livestock, leading to decreased immunity, impaired digestive function, and increased feed conversion ratios, among other problems.

[0003] Because young animals' digestive systems are not fully developed, their ability to digest milk and feed is weak, and their resistance is poor. They are easily affected by external factors, especially stressful events such as weaning, separation from their herd, or exposure to cold, which can easily cause cold-induced or stress-induced diarrhea. Furthermore, undigested nutrients ferment abnormally in the intestines, leading to an increase in intestinal pH, which causes opportunistic pathogens to proliferate uncontrollably, disrupting the body's flora balance and subsequently resulting in bacterial or viral infectious diarrhea.

[0004] Rotavirus, a genus of the Reoviridae family, is a significant pathogen causing acute viral gastroenteritis in infants and various young livestock and poultry. The disease is transmitted through the digestive tract. Infected young animals show obvious symptoms, primarily acute diarrhea. The disease is most prevalent during the cold season from late winter to early spring. Poor husbandry practices, inadequate hygiene, or concurrent pathogenic Escherichia coli infections can exacerbate the condition, increase mortality, and cause significant economic losses to the livestock industry. Current clinical treatment for rotavirus diarrhea in young animals primarily involves fluid replacement therapy to correct dehydration, antiviral drugs, and antibiotics. However, these methods have limitations, failing to effectively suppress the virus and easily disrupting the intestinal flora of young animals, thus worsening diarrhea. Traditional Chinese medicine, benefiting from its multi-target and multi-activity characteristics, has been applied to the treatment of various livestock and poultry diseases in recent years. Therefore, this invention aims to provide a convenient, safe, effective, and highly bioavailable traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs, along with its preparation method. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] To address the shortcomings of existing technologies, this invention provides a traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs, and its preparation method.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs. The traditional Chinese medicine preparation is made from the following traditional Chinese medicinal materials in parts by weight: 0.5-2 parts pomegranate peel, 1-2 parts pomegranate seeds, 3-6 parts white ochre, 3-6 parts red ochre, 3-6 parts halloysite, 1-2 parts montmorillonite, 1-2 parts cuttlebone, 0.5-2 parts licorice, 1-3 parts dried ginger, and 1-2 parts roasted malt.

[0010] Preferably, the traditional Chinese medicine preparation is made from the following traditional Chinese medicinal materials in parts by weight: 1 part pomegranate peel, 1 part pomegranate seeds, 3 parts white ochre, 3 parts red ochre, 3 parts halloysite, 1 part montmorillonite, 1 part cuttlebone, 2 parts licorice, 3 parts dried ginger, and 2 parts roasted malt.

[0011] Secondly, the present invention provides a method for preparing a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs, specifically including the following steps:

[0012] (1) Weigh out the white ochre, red ochre, gentian, montmorillonite and cuttlebone according to the above weight proportions, mix them, pulverize them with a rod mill and pass them through a 400-mesh sieve;

[0013] (2) Weigh the pomegranate peel, pomegranate seeds, licorice, dried ginger and roasted malt according to the above weight proportions, mix them and coarsely crush them, then crush them using an ultra-fine pulverizing dual airflow device and pass them through a 300-mesh sieve;

[0014] (3) After mixing the pulverized drugs from steps (1) and (2) evenly, the above-mentioned traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs is obtained.

[0015] Furthermore, the tannin content in the traditional Chinese medicine preparation shall not be less than 5%.

[0016] Introduction and pharmacological effects of each Chinese medicinal material in the traditional Chinese medicine preparation of this invention.

[0017] Pomegranate peel: The dried pericarp of the pomegranate plant (Punica granatum L.), belonging to the Punicaceae family; it has a sour and astringent taste and is warm in nature; it is used for chronic diarrhea, dysentery, hematochezia, rectal prolapse, metrorrhagia, leukorrhea, and abdominal pain due to intestinal parasites; it has the effects of astringing the intestines and stopping diarrhea, stopping bleeding, and expelling parasites. It is commonly used for chronic diarrhea, dysentery, hematochezia, rectal prolapse, metrorrhagia, leukorrhea, and abdominal pain due to intestinal parasites.

[0018] Pomegranate seeds: The dried seeds of the pomegranate plant (Pomegranate argentea), also known as pomegranate seeds in Tibetan medicine; they are sour, sweet, warm, and moist in nature; they are mainly used to treat cold-related ailments, stomach coldness, and stomach diseases, and have the effects of aiding digestion, fighting gastric ulcers, and lowering blood lipids and blood sugar.

[0019] White stone powder: a mineral-based traditional Chinese medicine derived from the silicate mineral kaolinite. Its main component is hydrated aluminum silicate. Also known as white talisman or white clay, it is neutral in nature and sweet and sour in taste. It enters the stomach and large intestine meridians and has the effects of astringing the intestines and stopping diarrhea, stopping bleeding and promoting tissue regeneration. Clinically, it is often used for chronic diarrhea, dysentery, metrorrhagia and leukorrhea.

[0020] Red ochre: A mineral-based traditional Chinese medicine, it is a silicate mineral, specifically hydrous kaolinite from the hydrous kaolinite group, primarily containing aluminum silicate tetrahydrate [Al4(Si4O3)2]. 10 [(OH)8•4H2O], enters the large intestine and stomach meridians, and has the effects of astringing the intestines and stopping diarrhea, astringing and stopping bleeding, promoting tissue regeneration and healing sores. It is often used to treat chronic diarrhea, hematochezia, and non-healing ulcers.

[0021] Yu Yu Liang: This is a hydroxide mineral called limonite, mainly containing basic iron oxide [FeO(OH)]. It has a sweet and astringent taste, and is slightly cold in nature; it enters the stomach and large intestine meridians; it has the effects of astringing the intestines and stopping diarrhea, and constricting and stopping bleeding. It is commonly used for chronic diarrhea and dysentery, rectal bleeding, metrorrhagia, and leukorrhea.

[0022] Montmorillonite: A natural aluminosilicate mineral, its main component is hydrated magnesium aluminum silicate, which is used in both traditional Chinese medicine and modern pharmaceuticals. It is neutral in nature and sweet in taste, and enters the stomach and large intestine meridians. It has the effects of adsorption and astringency, protection of mucous membranes, and antidiarrheal properties, and is often used to relieve diarrhea, gastrointestinal inflammation, and gastrointestinal bleeding.

[0023] Cuttlebone: The dried inner shell of the cuttlefish (Sepia esculenta) or the golden cuttlefish (Sepia esculenta), also known as cuttlebone or squid shell. It mainly contains calcium carbonate (80%-85%), shell keratin and mucilage. It is warm in nature, salty and astringent in taste, and enters the spleen and kidney meridians. It has the effects of astringing and stopping bleeding, astringing essence and stopping leukorrhea, neutralizing acid and relieving pain, and astringing and healing sores. It is mainly used to treat stomach pain and acid reflux, metrorrhagia and hematochezia, seminal emission and leukorrhea, and external bleeding.

[0024] Licorice: The dried root and rhizome of *Glycyrrhiza uralensis* Fisch., *Glycyrrhiza inflata* Bat., or *Glycyrrhiza glabra* L. (all belonging to the Fabaceae family). It has a sweet taste and neutral properties, and enters the heart, lung, spleen, and stomach meridians. It has the effects of tonifying the spleen and replenishing qi, clearing heat and detoxifying, resolving phlegm and relieving cough, relieving spasms and pain, and harmonizing other herbs. It is commonly used for spleen and stomach weakness, fatigue, palpitations, shortness of breath, cough with excessive phlegm, abdominal and limb spasms and pain, carbuncles and boils, and to alleviate the toxicity and harshness of other drugs.

[0025] Dried ginger: The dried rhizome of Zingiber ojjicinale Rosc., a plant of the ginger family. It is pungent and hot in nature, and enters the spleen, stomach, kidney, heart, and lung meridians. It warms the middle and dispels cold, restores yang and unblocks the meridians, warms the lungs and transforms phlegm. It is used for cold pain in the epigastrium and abdomen, vomiting and diarrhea, cold limbs and weak pulse, and cough with cold phlegm.

[0026] Roasted malt: A processed product of malt in traditional Chinese medicine, it is made by germinating and drying the mature fruit of barley (a plant of the Poaceae family), and then roasting it until it is charred. It has a sweet and slightly astringent taste and is warm in nature. It has the effects of promoting digestion, relieving stagnation, and stopping diarrhea. It is often used to treat food stagnation and diarrhea due to spleen deficiency.

[0027] (III) Beneficial Effects

[0028] 1. This invention is based on the theory of traditional Chinese veterinary medicine and proposes a traditional Chinese medicine formula for the prevention and treatment of rotavirus diarrhea in calves and lambs. The formula uses white ochre, red ochre, and halloysite as the principal ingredients. White ochre and red ochre have strong astringent and antidiarrheal effects, which can not only prevent intestinal prolapse but also absorb pathogens and dampness in the intestines, reducing the frequency of diarrhea. Moreover, their warm nature can also take into account the deficiency and coldness of the spleen and stomach, avoiding the retention of cold in the intestines. Halloysite has significant astringent and antidiarrheal effects. When combined with red and white ochre, the three ingredients work together as the principal ingredients, which can both astringe the intestines and stop diarrhea to treat the symptoms and take into account the qi of the spleen and stomach to protect the root cause. Furthermore, since the three are mineral medicines, after being ultra-finely pulverized, they will form a layered structure and a non-uniform charge distribution, which can fix and inhibit viruses, bacteria and their toxins and gases in the digestive tract, making them lose their pathogenicity. They can also form a protective layer on the surface of the gastrointestinal mucosa, protecting the gastrointestinal mucosa from damage by pathogenic factors. In addition, dried ginger and roasted malt are used as assistant herbs. Dried ginger is pungent and hot, with a strong ability to warm the middle and dispel cold. It can warm the yang of the spleen and stomach, dispel the deficiency and cold in the middle jiao, restore the digestive function of the spleen and stomach, and fundamentally solve the problems of spleen and stomach deficiency and cold and water retention. When combined with the principal herb, it can restrain the cooling and astringent properties of mineral medicines such as litharge and halloysite, prevent excessive astringency from causing stagnation, and at the same time warm the yang to help consolidate and astringe. Roasted malt is sweet and neutral, with significant effects of promoting digestion, eliminating food stagnation, strengthening the spleen and opening the stomach. When combined with dried ginger, one warms and the other eliminates, working together to warm the middle and strengthen the spleen, promote digestion and harmonize the stomach. It not only helps the principal herb stop diarrhea, but also prevents astringency from hindering the stomach. Then, pomegranate peel, pomegranate seeds, montmorillonite, and cuttlebone are used as adjuvant herbs. Pomegranate peel and seeds astringe the intestines and stop diarrhea; together with the principal herb, they enhance the effect of consolidating the intestines and stopping diarrhea. Moreover, their sour taste can astringe yin, which can alleviate the depletion of yin fluids caused by prolonged diarrhea in young animals. Montmorillonite, combined with mineral drugs such as red and white ochre, enhances the adsorption and astringent effects. Cuttlebone astringes and stops bleeding; its astringent properties help the principal herb stop diarrhea, and its warming properties are suitable for the syndrome of spleen and stomach deficiency and cold. Finally, licorice is used as the guiding herb to invigorate qi, replenish the middle jiao, clear heat and detoxify, and harmonize the effects of all the herbs.

[0029] Based on the above-mentioned formula, this invention prepares a traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs. Through extensive experimental screening of the drug composition and dosage, it has been further determined that the traditional Chinese medicine preparation is made from the following traditional Chinese medicinal materials in parts by weight: 1 part pomegranate peel, 1 part pomegranate seeds, 3 parts white ochre, 3 parts red ochre, 3 parts halloysite, 1 part montmorillonite, 1 part cuttlebone, 2 parts licorice, 3 parts dried ginger, and 2 parts roasted malt; it has the effects of astringing and stopping diarrhea, warming the middle and strengthening the spleen.

[0030] 2. The traditional Chinese medicine preparation of the present invention is prepared by using different ultra-micro pulverization processes for different medicinal materials. The traditional Chinese medicine preparation is convenient to administer, and the ultra-micro particle size can effectively improve the body's absorption and utilization rate of the drug and reduce the dosage.

[0031] 3. The traditional Chinese medicine preparation of this invention has a cure rate of up to 90% for rotavirus diarrhea in young animals, and a 100% rotavirus seroconversion rate. This traditional Chinese medicine preparation is effective against rotavirus diarrhea in young animals, has safe and mild ingredients, and has no toxic side effects on the body; therefore, it can be widely promoted and applied. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] Example 1

[0034] A traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs, the preparation being made according to the following steps:

[0035] (1) Take 3kg of white ochre, 3kg of red ochre, 3kg of halloysite, 1kg of montmorillonite and 1kg of cuttlebone, mix them together, pulverize them with a rod mill and pass them through a 400-mesh sieve;

[0036] (2) Take 1 kg of pomegranate peel, 1 kg of pomegranate seeds, 2 kg of licorice, 3 kg of dried ginger, and 2 kg of roasted malt, mix them together and coarsely grind them. Then, use an ultra-fine grinding dual-airflow device to grind them and mix them through a 300-mesh screen.

[0037] (3) Mix the 400-mesh mineral powder obtained in step (1) with the 300-mesh plant-based Chinese medicine powder obtained in step (2) evenly to obtain a Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves / lambs.

[0038] Example 2

[0039] A traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs, the preparation being made according to the following steps:

[0040] (1) Take 30kg of white ochre, 30kg of red ochre, 30kg of halloysite, 10kg of montmorillonite and 10kg of cuttlebone, mix them together, pulverize them with a rod mill and pass them through a 400-mesh sieve;

[0041] (2) Take 10 kg of pomegranate peel, 10 kg of pomegranate seeds, 20 kg of licorice, 30 kg of dried ginger, and 20 kg of roasted malt, mix them together and coarsely grind them. Then, use an ultra-fine grinding dual-airflow device to grind them and mix them through a 300-mesh screen.

[0042] (3) Mix the 400-mesh mineral powder obtained in step (1) with the 300-mesh plant-based Chinese medicine powder obtained in step (2) evenly to obtain a Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves / lambs.

[0043] Example 3

[0044] Screening of drug components in traditional Chinese medicine preparations according to this invention

[0045] 1 Experimental Drugs

[0046] Preparation 1: Red ochre, Halloysite, Montmorillonite, Cuttlebone, Pomegranate peel, Pomegranate seeds

[0047] Preparation 2: White ochre, red ochre, halloysite, montmorillonite, cuttlebone

[0048] Preparation 3: Pomegranate peel, pomegranate seeds, licorice, dried ginger, roasted malt

[0049] Preparation 4: Montmorillonite, Cuttlebone, Pomegranate Peel, Pomegranate Seed, Licorice, Dried Ginger

[0050] Preparation 5: White ochre, red ochre, halloysite, montmorillonite, cuttlebone, pomegranate peel, pomegranate seeds, licorice, dried ginger, roasted malt

[0051] Each medicinal material is prepared in 1 part by weight, with each part by weight being 1 kg. Preparations 1-5 were obtained according to the method described in Example 1.

[0052] 2. Experimental Methods

[0053] Fifty-one calves exhibiting diarrhea symptoms and testing positive for rotavirus were selected from a ranch in Ningxia. They were randomly divided into five groups (groups 1-5), receiving 50g / calves of rotavirus preparation 1-5 mixed with milk for four consecutive days. The diarrhea pattern was observed daily, and the cure rate and effectiveness rate were calculated. On day 5, rotavirus antigen testing was performed, and the cure, improvement, and effectiveness rates were statistically analyzed. Cure rate = number of cured calves / number of trials. Effective rate = (number of cured calves + number of improved calves) / number of trials.

[0054] Therapeutic criteria:

[0055] Cure: After treatment, the diarrhea symptoms disappeared, the patient's mental state and appetite were good, the body temperature was normal, the stool was formed, the area around the anus was dry, and the rotavirus rapid test strip test was negative;

[0056] Improvement: Significant improvement in watery diarrhea symptoms or no loose stools, better appetite or mental state, negative test strip results, but not fully recovered by day 5;

[0057] Ineffective: Cattle that show no improvement on the 5th day of observation, still have watery feces or diarrhea in the pen, or are in poor spirits or have a poor appetite.

[0058] 3 Results

[0059] As can be seen from the results in Table 1, the diarrhea cure rate and rotavirus seroconversion rate of formulation 5 were the highest, indicating that the combination of mineral drugs, warming drugs and astringent drugs in formulation 5 has a more definite astringent and consolidating effect, warming the middle and strengthening the spleen. Therefore, the drug composition of formulation 5 in this embodiment is determined to be the basic composition for subsequent studies.

[0060] Table 1. The therapeutic effects of traditional Chinese medicine preparations with different drug compositions on rotavirus diarrhea in calves [n (%)]

[0061]

[0062] Example 4

[0063] Screening of drug dosage in traditional Chinese medicine preparations in this invention

[0064] 1. Experimental drug

[0065] Preparation 1: 1 part white ochre, 1 part red ochre, 1 part halloysite, 1 part montmorillonite, 1 part cuttlebone, 1 part pomegranate peel, 1 part pomegranate seeds, 1 part licorice, 1 part dried ginger, 1 part roasted malt

[0066] Preparation 2: 1 part white ochre, 1 part red ochre, 1 part halloysite, 1 part montmorillonite, 1 part cuttlebone, 2 parts pomegranate peel, 2 parts pomegranate seeds, 2 parts licorice, 2 parts dried ginger, 2 parts roasted malt

[0067] Preparation 3: 3 parts white ochre, 3 parts red ochre, 3 parts halloysite, 1 part montmorillonite, 1 part cuttlebone, 1 part pomegranate peel, 1 part pomegranate seed, 2 parts licorice, 3 parts dried ginger, 2 parts roasted malt.

[0068] Preparation 4: 3 parts white ochre, 3 parts red ochre, 3 parts halloysite, 3 parts montmorillonite, 1 part cuttlebone, 1 part pomegranate peel, 1 part pomegranate seed, 1 part licorice, 1 part dried ginger, 1 part roasted malt

[0069] Each medicinal material was prepared in 1 part by weight, with each part by weight being 1 kg. Preparations 1-4 were obtained according to the method described in Example 1.

[0070] 2. Experimental Methods

[0071] Forty calves exhibiting diarrhea symptoms and testing positive for rotavirus were selected from a ranch in Inner Mongolia. They were randomly divided into four groups (Groups 1-4), receiving 50g / calves of rotavirus-prescribed preparations (1-4) mixed with milk for four consecutive days. The diarrhea pattern was observed daily, and the cure rate and effectiveness rate were calculated. On day 5, rotavirus antigen testing was performed, and the cure, improvement, and effectiveness rates were statistically analyzed. Cure rate = Number of cured calves / Number of trials. Effective rate = (Number of cured calves + Number of improved calves) / Number of trials.

[0072] Therapeutic criteria:

[0073] Cure: After treatment, the diarrhea symptoms disappeared, the patient's mental state and appetite were good, the body temperature was normal, the stool was formed, the area around the anus was dry, and the rotavirus rapid test strip test was negative;

[0074] Improvement: Significant improvement in watery diarrhea symptoms or no loose stools, better appetite or mental state, negative test strip results, but not fully recovered by day 5;

[0075] Ineffective: Cattle that show no improvement on the 5th day of observation, still have watery feces or diarrhea in the pen, or are in poor spirits or have a poor appetite.

[0076] 3 Results

[0077] As shown in Table 2, formulation 3 had the highest cure rate and total effective rate for diarrhea. The effective rate of formulation 2 was higher than that of formulation 4, and the cure rate of formulation 4 was higher than that of formulation 2. This may be because the dosage of astringent mineral drugs such as white ochre, red ochre, and maltose, as well as the warming drug dried ginger in formulation 3 was higher than that in formulations 4 and 2. Therefore, formulation 3 in this embodiment was determined as the final formulation for the prevention and treatment of calf rotavirus diarrhea.

[0078] Table 2. The therapeutic effects of traditional Chinese medicine preparations with different drug compositions on rotavirus diarrhea in calves [n (%)]

[0079]

[0080] Example 5

[0081] The therapeutic effect of the traditional Chinese medicine preparation of this invention on calf rotavirus diarrhea.

[0082] Twenty-seven calves aged 15-25 days from a large dairy farm in Ningxia were selected. These calves exhibited watery diarrhea (gray, green, or yellow) or feces containing intestinal mucus, and tested positive for rotavirus antigen. Fifteen calves were in the experimental group, receiving 40-50g of the traditional Chinese medicine preparation described in Example 2 daily, mixed with milk. The other 12 calves were in the control group, receiving the farm's standard treatment (20g of Bai Tou Weng powder mixed with milk / day + 2.5mg / kg body weight of enrofloxacin injection) once daily for four consecutive days. The diarrhea pattern was observed daily, and the cure rate and effectiveness rate were calculated. Rotavirus antigen testing was performed on day 5, and the cure, improvement, and effectiveness rates were calculated. Cure rate = number of cured calves / number of trials. Effective rate = (number of cured calves + number of improved calves) / number of trials.

[0083] Therapeutic criteria:

[0084] Cure: After treatment, the diarrhea symptoms disappeared, the patient's mental state and appetite were good, the body temperature was normal, the stool was formed, the area around the anus was dry, and the rotavirus rapid test strip test was negative;

[0085] Improvement: Significant improvement in watery diarrhea symptoms or no loose stools, better appetite or mental state, negative test strip results, but not fully recovered by day 5;

[0086] Ineffective: Cattle that show no improvement on the 5th day of observation, still have watery feces or diarrhea in the pen, or are in poor spirits or have a poor appetite.

[0087] The results are shown in Table 3. After the administration of the drug, the cure rate, efficacy rate and rotavirus seroconversion rate of the experimental group were all higher than those of the enrofloxacin control group. In particular, the rotavirus seroconversion rate of the experimental group was 100%, which shows that the traditional Chinese medicine preparation of the present invention has a significant therapeutic effect on rotavirus diarrhea in calves.

[0088] Table 3. Therapeutic effect of the traditional Chinese medicine preparation of this invention on calf rotavirus diarrhea [n (%)]

[0089] Example 5

[0090] The therapeutic effect of the traditional Chinese medicine preparation of this invention on calf rotavirus diarrhea.

[0091] Thirty calves at a dairy farm in Helan Mountain Dairy Industry, Ningxia State Farms, tested positive for rotavirus antigen and presented with watery diarrhea (gray, green, or yellow) or feces containing intestinal mucus. They were randomly divided into three groups: a 10-calves group receiving 50g of the herbal preparation from Example 2 daily; a 10-calves control group receiving routine treatment (20g of *Pulsatilla chinensis* powder mixed with milk daily + 2.5mg / kg body weight of enrofloxacin injection); and a 10-calves group receiving combined treatment (2.5mg / kg body weight of enrofloxacin injection and 50g of the herbal preparation from Example 2). All calves were given the medication mixed with milk once daily for four days. The diarrhea pattern was observed daily, and the cure rate and effectiveness rate were calculated. Rotavirus antigen testing was performed on the fifth day, and the cure, improvement, and effectiveness rates were calculated. Cure rate = number of cured calves / number of trials. Effective rate = (number of cured calves + number of improved calves) / number of trials.

[0092] Therapeutic criteria:

[0093] Cure: After treatment, the diarrhea symptoms disappeared, the patient's mental state and appetite were good, the body temperature was normal, the stool was formed, the area around the anus was dry, and the rotavirus rapid test strip test was negative;

[0094] Improvement: Significant improvement in watery diarrhea symptoms or no loose stools, better appetite or mental state, negative test strip results, but not fully recovered by day 5;

[0095] Ineffective: Cattle that show no improvement on the 5th day of observation, still have watery feces or diarrhea in the pen, or are in poor spirits or have a poor appetite.

[0096] The results are shown in Table 4. After administration of the traditional Chinese medicine preparation group and the enrofloxacin + traditional Chinese medicine preparation group, the cure rate, efficacy rate and rotavirus seroconversion rate of diarrhea were all higher than those of the conventional administration control group. This indicates that the traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves can be used to treat rotavirus infection in calves, especially for curing diarrhea symptoms and achieving rotavirus antigen seroconversion.

[0097] Table 4. Therapeutic effect of the traditional Chinese medicine preparation of this invention on calf rotavirus diarrhea [n (%)]

[0098]

[0099] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine preparation for the prevention and treatment of rotavirus diarrhea in calves and lambs, characterized in that, The traditional Chinese medicine preparation is made from the following medicinal materials in parts by weight: 0.5-2 parts pomegranate peel, 1-2 parts pomegranate seeds, 3-6 parts white ochre, 3-6 parts red ochre, 3-6 parts halloysite, 1-2 parts montmorillonite, 1-2 parts cuttlebone, 0.5-2 parts licorice, 1-3 parts dried ginger, and 1-2 parts roasted malt.

2. The traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs according to claim 1, characterized in that, The traditional Chinese medicine preparation is made from the following traditional Chinese medicinal materials in parts by weight: 1 part pomegranate peel, 1 part pomegranate seeds, 3 parts white ochre, 3 parts red ochre, 3 parts halloysite, 1 part montmorillonite, 1 part cuttlebone, 2 parts licorice, 3 parts dried ginger, and 2 parts roasted malt.

3. A method for preparing a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs as described in any one of claims 1 to 2, characterized in that, Specifically, the following steps are included: (1) Weigh out the white ochre, red ochre, gentian, montmorillonite and cuttlebone according to the above weight proportions, mix them, pulverize them with a rod mill and pass them through a 400-mesh sieve; (2) Weigh the pomegranate peel, pomegranate seeds, licorice, dried ginger and roasted malt according to the above weight proportions, mix them and coarsely crush them, then crush them using an ultra-fine pulverizing dual airflow device and pass them through a 300-mesh sieve; (3) After mixing the pulverized drugs from steps (1) and (2) evenly, the above-mentioned traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs is obtained.

4. The method for preparing a traditional Chinese medicine preparation for preventing and treating rotavirus diarrhea in calves and lambs as described in claim 3, characterized in that, The tannin content in the traditional Chinese medicine preparation prepared by the method shall not be less than 5%.

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