Optimized formula of natural nano organic plant selenium with higher bioavailability and stronger anticancer activity and preparation method of natural nano organic plant selenium

Abstract

The invention discloses an optimized formula of natural nano organic plant selenium with higher bioavailability and stronger anti-cancer activity and a preparation method, and belongs to the technical field of functional food and nutrient enrichment. According to the formula, L-sodium ascorbate, selenium-enriched yeast, L-ascorbic acid, D-biotin and L-selenium-methyl selenocysteine are used as core raw materials, and edible corn starch, maltodextrin, magnesium stearate and hydroxypropyl starch empty capsules are compounded to prepare capsules. Organic selenium is converted into a natural nano state with uniform particle size through processes of reduction stabilization, nano dispersion, low-temperature homogenization and step-by-step granulation, so that the solubility, the absorptivity and the bioavailability are remarkably improved; all the active ingredients have a synergistic effect, so that the selenium toxicity is reduced, and the antioxidant and in-vitro anticancer activity is enhanced. The problems of poor absorption, insufficient stability, single activity and the like of traditional organic selenium are solved, and the product is safe and stable, is suitable for long-term administration and can be used for anticancer assistance, immune enhancement and oxidative stress protection.

Description

Technical Field

[0001] This invention relates to the fields of functional foods, nutritional fortifiers, and pharmaceutical excipients, specifically to an optimized formulation of natural nano-organic plant selenium with higher bioavailability and stronger anti-cancer activity, and its preparation method. Background Technology

[0002] Selenium is an essential trace element for the human body, participating in key physiological processes such as antioxidation, immune regulation, and anti-tumor activity. Traditional inorganic selenium is highly toxic and has a narrow safety window; common organic selenium (selenium-enriched yeast, selenoamino acids) suffers from slow absorption, low bioavailability, poor stability, and uneven distribution in the body, limiting its anti-cancer and health-promoting effects.

[0003] Nanoparticles can significantly improve solubility, dissolution rate, and cellular uptake; however, most existing nano-selenium is prepared by chemical reduction, resulting in insufficient purity, safety, and naturalness. Furthermore, a single selenium source is insufficient to achieve efficient absorption and synergistic anti-cancer effects.

[0004] To address the aforementioned deficiencies, this invention utilizes L-seleno-methylselenocysteine ​​and selenium-enriched yeast as dual organic selenium sources, combined with an L-ascorbic acid / L-ascorbic acid sodium reduction stabilization system and D-biotin to promote absorption. Through nano-processing, it is prepared into capsules, achieving a natural plant selenium preparation with high bioavailability, low toxicity, and strong anti-cancer activity. Summary of the Invention

[0005] 1. Technical problems to be solved (1) Improve the solubility, dissolution rate and bioavailability of organic selenium; (2) Enhances the antioxidant and in vitro anticancer activity of selenium preparations and reduces toxicity; (3) Improved stability, suitable for industrialization and long-term storage; (4) Provide scalable, mild and green preparation processes. Technical solution

[0006] Formula (parts by weight) -Core ingredients: Sodium L-ascorbate 5–15, selenium-enriched yeast 10–25, L-ascorbic acid 8–20, D-biotin 0.01–0.05, L-seleno-methylselenocysteine ​​2–8 -Excipients: 20-40g edible corn starch, 15-30g maltodextrin, 0.5-2g magnesium stearate, and an appropriate amount of hydroxypropyl starch empty capsules. Preparation process Raw material sieving → Reduction stabilization (pH 4.0–6.0, 30–50℃) → High-pressure homogenization / ultrasonic nano-dispersion → Low-temperature drying → Total mixing → Capsule filling → Finished product Beneficial effects

[0007] (1) High bioavailability: Nano-sizing significantly improves dissolution and transmembrane absorption, and bioavailability is ≥30% higher than that of ordinary organic selenium.

[0008] (2) Strong anti-cancer activity: The synergistic effect of dual selenium source and vitamin enhances the repair of oxidative damage and inhibits the proliferation of tumor cells.

[0009] (3) Safe and stable: Natural organic selenium + mild process, low toxicity, good stability, suitable for long-term use.

[0010] (4) Controllable process: Particle size 100–500 nm, good uniformity, suitable for large-scale industrial production. Detailed Implementation

[0011] Example 1 (Preferred Formula) -Ingredients: 10 parts sodium L-ascorbate, 18 parts selenium-enriched yeast, 12 parts L-ascorbic acid, 0.03 parts D-biotin, 4 parts L-seleno-methylselenocysteine -Excipients: 30 parts edible corn starch, 22 parts maltodextrin, 1 part magnesium stearate, and an appropriate amount of hydroxypropyl starch empty capsules. -preparation: 1. Pass through a 100-mesh sieve; 2. Prepare a 15% aqueous solution of L-ascorbic acid and sodium L-ascorbate, pH 5.0, and stir at 40℃ for 40 min; 3. Add D-biotin, homogenize at 70 MPa for 15 min, D50≈280 nm; 4. Spray drying (inlet air 140℃, outlet 70℃), moisture content ≤4.0%; 5. Total mixture and filler capsules. Example

[0012] -Ingredients: 8 parts sodium L-ascorbate, 15 parts selenium-enriched yeast, 10 parts L-ascorbic acid, 0.02 parts D-biotin, 3 parts L-selenomethylselenocysteine -Ingredients: 25 parts edible corn starch, 20 parts maltodextrin, 0.8 parts magnesium stearate -Preparation: Ultrasound 600 W × 20 min, D50≈320 nm. Example

[0013] -Ingredients: 12 parts sodium L-ascorbate, 20 parts selenium-enriched yeast, 15 parts L-ascorbic acid, 0.04 parts D-biotin, 6 parts L-seleno-methylselenocysteine -Ingredients: 35 parts edible corn starch, 25 parts maltodextrin, 1.2 parts magnesium stearate -Preparation: Homogenize at 90 MPa for 12 min, D50≈220 nm.

[0014] V. Performance Verification - Particle size: D50 = 100–500 nm, PDI ≤ 0.3; -Dissolution: ≥85% in 45 minutes; -Bioavailability: The AUC in rats was ≥35% higher than that of ordinary selenium-enriched yeast; -In vitro anticancer effect: The inhibition rate of liver cancer and lung cancer cells was significantly higher than that of the single selenium source group; -Stability: After 6 months of accelerated testing, there was no significant decrease in content, particle size, or dissolution.

[0015] VI. Scope of Application This product can be used as a highly bioavailable selenium supplement, an antioxidant functional food, and an anti-cancer auxiliary nutritional supplement. It is suitable for people with selenium deficiency, people with low immunity, and people at high risk of or recovering from cancer.

[0016] VII. Conclusion This invention utilizes a dual organic selenium source + reduction stabilization system + nanotechnology + plant capsules to achieve a natural, safe, and highly efficient organic plant selenium preparation. It possesses both high bioavailability and strong anti-cancer activity, and the process is green and controllable, showing promising prospects for industrialization and application.

Claims

1. A natural nano-organic plant selenium composition, characterized in that, Made from the following parts by weight of raw materials: 5–15 parts of sodium L-ascorbate, 10–25 parts of selenium-enriched yeast, 8–20 parts of L-ascorbic acid, 0.01–0.05 parts of D-biotin, and 2–8 parts of L-seleno-methylselenocysteine. The excipients are: 20–40 parts edible corn starch, 15–30 parts maltodextrin, 0.5–2 parts magnesium stearate, and an appropriate amount of hydroxypropyl starch empty capsules.

2. The composition according to claim 1, characterized in that, The preferred composition by weight is: 8–12 parts of sodium L-ascorbate, 15–20 parts of selenium-enriched yeast, 10–15 parts of L-ascorbic acid, 0.02–0.04 parts of D-biotin, and 3–6 parts of L-seleno-methylselenocysteine. 25–35 parts corn starch, 20–25 parts maltodextrin, and 0.8–1.5 parts magnesium stearate.

3. The composition according to claim 1, characterized in that, The selenium-enriched yeast has an organic selenium content ≥1000 μg / g and an L-seleno-methylselenocysteine ​​purity ≥98%.

4. The composition according to claim 1, characterized in that, The product is a hydroxypropyl starch empty capsule containing nano-sized organic selenium composite powder with a median particle size D50 of 100–500 nm.

5. A method for preparing the natural nano-organic plant selenium composition according to any one of claims 1-4, characterized in that, Includes the following steps: (1) Raw material pretreatment: Sodium L-ascorbate, L-ascorbic acid, D-biotin, L-seleno-methylselenocysteine ​​and selenium-enriched yeast were passed through an 80–120 mesh sieve and set aside for later use; (2) Reduction and stabilization: L-ascorbic acid and sodium L-ascorbate were dissolved in purified water to prepare a 10%–20% aqueous solution. The pH was adjusted to 4.0–6.

0. ​​Selenium-enriched yeast and L-seleno-methylselenocysteine ​​were added and stirred at 30–50℃ for 30–60 min to form a stable selenium precursor solution. (3) Nano-dispersion: D-biotin is added and homogenized / dispersed under high pressure at 50–100 MPa or ultrasonic power at 300–800 W for 10–30 min to obtain a nano-selenium dispersion. (4) Low temperature drying: spray drying / vacuum freeze drying, inlet air temperature 120–160℃, outlet temperature 60–80℃, to obtain nanocomposite powder; (5) Mixing: Mix nano powder with edible corn starch and maltodextrin, mix at 30–60 rpm for 15–30 min, add magnesium stearate and mix for another 5–10 min; (6) Filled capsules: Filled with hydroxypropyl starch empty capsules, sterilized, inspected and packaged to obtain the finished product.

6. The preparation method according to claim 5, characterized in that, The nano-dispersion obtained in step (3) has a particle size of 100–500 nm and a polydispersity index (PDI) ≤ 0.

3.

7. The preparation method according to claim 5, characterized in that, The moisture content of the dried powder is ≤5.0%.

8. The use of the composition according to any one of claims 1–4 in the preparation of highly bioavailable selenium supplements, antioxidant functional foods, and anticancer adjuvant nutritional preparations.