A facial mask with whitening, moisturizing and freckle-removing functions and its preparation method

Abstract

This application relates to the field of health product technology, specifically disclosing a facial mask with whitening, moisturizing, and freckle-removing functions and its preparation method. This facial mask with whitening, moisturizing, and freckle-removing functions comprises the following components in parts by weight: 15-25 parts of peach blossom Bletilla striata polysaccharide complex, 5-12 parts of silkworm pupae enzymatically hydrolyzed oligopeptides, 10-20 parts of biomimetic membrane-modified starch microspheres, 35-50 parts of winter melon juice lactic acid bacteria fermentation supernatant, and 1-3 parts of antioxidant complex. This application uses peach blossom Bletilla striata polysaccharide complex, silkworm pupae enzymatically hydrolyzed oligopeptides, biomimetic membrane-modified starch microspheres, winter melon juice lactic acid bacteria fermentation supernatant, and antioxidant complex in a compounded manner according to their weight proportions. The complex and oligopeptides exist in a molecular-level dispersion state, the microspheres act as carriers to uniformly carry the active components, and the antioxidant complex maintains the stability of the system. Therefore, it achieves the effects of highly dispersed active ingredients, sufficient contact with the skin during application, and improved retention rate of active substances after washing.

Description

Technical Field

[0001] This application relates to the field of health product technology, and more specifically, it relates to a facial mask with whitening, moisturizing and spot-removing functions and its preparation method. Background Technology

[0002] As people's living standards improve, consumers' demand for skin care products is increasing, especially skin care products with whitening, moisturizing, and skin brightening effects. As a convenient and efficient skin care product, face masks can allow effective ingredients to fully contact the skin for a longer period of time by covering the facial skin, thereby playing a role in nourishing and improving skin texture. At present, there are a variety of face mask products with whitening and moisturizing effects on the market, including sheet masks, cream masks, and powder masks.

[0003] In the field of natural skincare, powder masks are favored by some consumers due to their natural ingredients and reasonable formulation. They typically involve grinding ingredients such as peach blossom, Bletilla striata, and silkworm pupae into fine powders, mixing them evenly in a certain proportion, and then having consumers add water, milk, honey, or fresh fruit and vegetable juice to make a paste before applying it to the face. However, traditional powder masks are mostly in the form of raw herbal powders, and their active ingredients are encapsulated by plant cell walls. When using them, simply adding water or fruit juice makes it difficult to fully release the active ingredients into the liquid phase, which can lead to a low absorption and utilization rate of the active ingredients by the skin. Most of the active substances are washed away during washing, resulting in a limited proportion that actually works. Summary of the Invention

[0004] To address the problems of existing face masks, which make it difficult to fully release active ingredients into the liquid phase, resulting in low absorption and utilization rates of effective ingredients by the skin and the fact that most active substances are washed away during washing, thus limiting the actual effectiveness of the product, this application provides a face mask with whitening, moisturizing, and spot-removing functions, as well as its preparation method.

[0005] This application provides a facial mask with whitening, moisturizing, and freckle-removing functions, and its preparation method, which adopts the following technical solution: In the first aspect, this application provides a facial mask with whitening, moisturizing, and blemish-removing functions, employing the following technical solution: A facial mask with whitening, moisturizing and spot-removing functions comprises the following components in parts by weight: 15-25 parts of peach blossom Bletilla striata polysaccharide complex, 5-12 parts of silkworm enzymatic hydrolysis oligopeptide, 10-20 parts of biomimetic membrane modified starch microspheres, 35-50 parts of winter melon juice lactic acid bacteria fermentation supernatant, and 1-3 parts of antioxidant complex.

[0006] By adopting the above technical solution, the complex of peach blossom and Bletilla striata polysaccharide complex, Bombyx mori enzymatic hydrolysis oligopeptides, biomimetic membrane-modified starch microspheres, winter melon juice lactic acid bacteria fermentation supernatant, and antioxidant complex are compounded in parts by weight. The complex and oligopeptides exist in a molecular-level dispersion state, the microspheres act as carriers to uniformly carry the active components, the fermentation broth makes each component form a fine mud-like paste, and the antioxidant complex maintains the stability of the system. Therefore, the active ingredients are highly dispersed, fully contact the skin during application, and the retention rate of active substances is improved after washing. This solves the problem of existing masks, which make it difficult to fully release active ingredients into the liquid phase, resulting in low absorption and utilization rate of effective ingredients by the skin, and most of the active substances are taken away during washing, resulting in a limited proportion of actual effects.

[0007] Preferably, the peach blossom and Bletilla striata polysaccharide complex is formed by complexing peach blossom extract and Bletilla striata polysaccharide extract at a dry matter weight ratio of 1:2-3. The peach blossom extract is an extract obtained by subcritical water extraction of peach blossoms, and the Bletilla striata polysaccharide extract is a polysaccharide extract obtained by enzyme-assisted water extraction of Bletilla striata.

[0008] By adopting the above technical solution, the flavonoids in peach blossom extract and the polysaccharide molecules in Bletilla striata polysaccharide extract form molecular-level complexes through hydrogen bonds, which improves the dispersibility and stability of flavonoids in the aqueous phase. At the same time, the adhesiveness of polysaccharides helps the active ingredients to remain on the skin surface, thereby improving the transdermal absorption efficiency and whitening effect of the mask.

[0009] Preferably, the biomimetic membrane-modified starch microspheres comprise, from the inside out, a porous starch core, a biomimetic lipid membrane layer coating the surface of the porous starch core, and a chitosan oligosaccharide hydrophilic layer sprayed onto the surface of the biomimetic lipid membrane layer. The porous starch core has a particle size of 20-50 micrometers and a porosity of 40-60%. The biomimetic lipid membrane layer is composed of soybean lecithin and cholesterol in a molar ratio of 5-8:1. The chitosan oligosaccharide hydrophilic layer has a thickness of 10-50 nm and a molecular weight of 1000-3000 Da for the chitosan oligosaccharide.

[0010] By adopting the above technical solution, the biomimetic lipid membrane layer can fuse with the lipids of the stratum corneum of the skin to promote the penetration of active ingredients. The positively charged hydrophilic layer of chitosan oligosaccharide can be electrostatically adsorbed on the skin surface to achieve microsphere anchoring. The porous starch core enables the slow release of active ingredients. The three-layer structure works together to enable the active ingredients to continue to play a role after washing, thereby improving the long-lasting moisturizing and continuous whitening effect of the mask.

[0011] Preferably, the supernatant of the winter melon juice lactic acid bacteria fermentation is the supernatant obtained by centrifugation and filtration after the original winter melon juice has been fermented by mixed Lactobacillus plantarum and Streptococcus thermophilus, and the pH of the fermentation supernatant is 4.0-4.5.

[0012] By adopting the above technical solution, the organic acids produced by the fermentation of winter melon juice with lactic acid bacteria can temporarily regulate the permeability of the stratum corneum, creating favorable conditions for the penetration of microspheres. At the same time, the small molecule organic acids, free amino acids and polysaccharides produced by fermentation can synergistically enhance the water retention capacity of the stratum corneum, thereby improving the penetration and moisturizing effects of the mask.

[0013] Preferably, the antioxidant complex is composed of tea extract, rosemary extract and vitamin E succinate mixed in a weight ratio of 2-4:1:1 and then encapsulated with hydroxypropyl-β-cyclodextrin, and the average particle size of the antioxidant complex is 100-300 nm.

[0014] By adopting the above technical solution, EGCG in tea extract, carrageenan in rosemary extract, and vitamin E succinate work synergistically to exert antioxidant effects. After being encapsulated with cyclodextrin, their dispersibility and stability in the aqueous phase are improved, which can effectively protect the flavonoids and polysaccharides in the mask from oxidative degradation, thereby maintaining the efficacy stability of the product during storage and use.

[0015] Secondly, this application provides a method for preparing a facial mask with whitening, moisturizing, and freckle-removing functions, using the following technical solution: A method for preparing a facial mask with whitening, moisturizing, and blemish-removing functions, applicable to the aforementioned facial mask with whitening, moisturizing, and blemish-removing functions, includes the following steps: S1. Peach blossom Bletilla striata polysaccharide complex, white silkworm enzymatic hydrolysis oligopeptide and biomimetic membrane modified starch microspheres were prepared sequentially. S2. Load the peach blossom Bletilla striata polysaccharide complex and the silkworm enzymatic hydrolysis oligopeptide onto the biomimetic membrane-modified starch microspheres to obtain microspheres loaded with active ingredients. S3. Prepare the supernatant of winter melon juice lactic acid bacteria fermentation and the antioxidant complex in sequence; S4. Mix the microspheres loaded with active ingredients with the antioxidant complex evenly, add the supernatant of the winter melon juice lactic acid bacteria fermentation, homogenize, fill, sterilize, and obtain a face mask.

[0016] By adopting the above technical solution and coordinating the various steps, the structural integrity of the active ingredients is ensured during the loading and mixing process, so that the final product forms a mud-like paste with highly dispersed active ingredients and uniformly distributed microspheres, thereby achieving the convenience of immediate use and stable efficacy release.

[0017] Preferably, in step S1, the preparation of the peach blossom Bletilla striata polysaccharide complex includes the following steps: Take dried peach blossoms, add deionized water at a material-to-liquid ratio of 1:10-15 g / mL, and extract in subcritical water at 120-140℃ and 2-4MPa for 30-60 minutes. Centrifuge and concentrate to obtain concentrated peach blossom extract. Take dried Bletilla striata, add deionized water at a material-to-liquid ratio of 1:20-30 g / mL, adjust the pH to 5.0-5.5, add a complex enzyme of cellulase and pectinase, enzymatically hydrolyze at 45-55℃ for 2-3 hours, inactivate the enzyme, centrifuge, concentrate, precipitate with alcohol, and dry to obtain Bletilla striata polysaccharide extract. Peach blossom extract concentrate and Bletilla striata polysaccharide extract were mixed at a dry matter weight ratio of 1:2-3, the pH was adjusted to 5.0-5.5, and the mixture was reacted at 45-55℃ for 2-3 hours. The mixture was then spray-dried to obtain peach blossom Bletilla striata polysaccharide complex. The preparation steps of the enzymatic hydrolysis oligopeptide of *Bombyx mori* are as follows: take dried *Bombyx mori*, add deionized water at a material-to-liquid ratio of 1:10-15 g / mL, adjust the pH to 7.0-7.5, add neutral protease, enzymatically hydrolyze at 50-55℃ for 3-4 hours, inactivate the enzyme, centrifuge, collect the supernatant, pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, collect the permeate, concentrate, spray dry, and obtain enzymatic hydrolysis oligopeptide of *Bombyx mori*. The preparation of the biomimetic membrane-modified starch microspheres includes the following steps: After gelatinizing glutinous corn starch, it is mixed and emulsified with an oil phase, crosslinked with epichlorohydrin, washed, and dried to obtain a porous starch core. The porous starch core has a particle size of 20-50 micrometers and a porosity of 40-60%. Soybean lecithin and cholesterol were mixed at a molar ratio of 5-8:1, dissolved in anhydrous ethanol, and the starch porous core was added. The mixture was stirred, the solvent was removed, and the mixture was dried to obtain biomimetic lipid membrane-coated microspheres. The biomimetic lipid membrane-coated microspheres were placed in a fluidized bed, sprayed with chitosan oligosaccharide solution, and dried to obtain biomimetic membrane-modified starch microspheres.

[0018] By adopting the above technical solutions, subcritical water extraction is used to efficiently enrich peach blossom flavonoids, enzyme-assisted water heating is used to obtain Bletilla striata polysaccharide, spray drying is used to ensure the activity of the complex, ultrafiltration membrane separation is used to obtain oligopeptides of suitable molecular weight, reverse emulsion polymerization is used to prepare uniform porous starch microspheres, and fluidized bed spraying is used to form a uniform chitosan oligosaccharide layer, thereby ensuring the yield and activity of each intermediate.

[0019] Preferably, in step S2, the load includes the following steps: The polysaccharide complex of Bletilla striata and the enzymatically hydrolyzed oligopeptide of Bombyx mori were mixed at a weight ratio of 3-4:1 and dissolved in phosphate buffer solution at pH 5.5-6.0 to prepare a 10-15% solution. Modified starch microspheres with biomimetic membrane were added to the solution at a mass ratio of 1:5-10. After immersion in the solution under a vacuum of 0.08-0.09 MPa for 25-35 minutes, the microspheres were collected by centrifugation and then vacuum dried at 35-45°C for 7-9 hours to obtain microspheres loaded with active ingredients.

[0020] By adopting the above technical solution, vacuum impregnation allows the active ingredients to enter the pores of the starch porous core, and the mild drying conditions avoid thermal damage to the active ingredients, thereby achieving high drug loading and sustained release performance, and ensuring the long-term effect of the microspheres on the skin surface.

[0021] Preferably, in step S3, the preparation of the supernatant from the lactic acid bacteria fermentation of winter melon juice includes the following steps: Take a fresh winter melon, peel and remove the seeds, juice it, filter it, and obtain the original winter melon juice; Add 5-10% glucose to the original winter melon juice, adjust the pH to 6.0-6.5, and sterilize. Inoculate with Lactobacillus plantarum and Streptococcus thermophilus, with a total inoculum of 1-2%, and ferment at 37°C for 24-36 hours until the pH drops to 4.0-4.5; Centrifuge, collect the supernatant, filter and sterilize to obtain the supernatant from the lactic acid bacteria fermentation of winter melon juice.

[0022] By adopting the above technical solution, the mixed strains ferment to produce a suitable ratio of lactic acid and acetic acid, which stabilizes the pH of the fermentation broth at 4.0-4.5. This acidic environment is conducive to the protonation of chitosan oligosaccharides on the surface of microspheres to enhance electrostatic adsorption, and can also gently promote the metabolism of the cuticle. At the same time, filtration and sterilization ensure the microbial safety of the product.

[0023] Preferably, in step S4, the mixing is performed by mixing the microspheres loaded with the active ingredients and the antioxidant complex at 100-200 rpm for 10-15 minutes to form a mixed powder. The homogenization is performed by adding the supernatant from the winter melon juice lactic acid bacteria fermentation to a vacuum homogenizer and stirring at 50-100 rpm, adding the mixed powder, vacuuming to 0.06-0.08 MPa, and homogenizing at 3000-5000 rpm for 5-10 minutes to form a paste. The sterilization is performed using... 60 Sterilization by Co-γ irradiation, with an irradiation dose of 5-10 kGy.

[0024] By adopting the above technical solution, the combination of low-speed premixing and high-speed vacuum homogenization enables the microspheres and antioxidant complexes to be evenly dispersed in the fermentation broth, forming a fine and stable mud-like paste. Irradiation sterilization is carried out at room temperature, avoiding the damage of active ingredients to heat sterilization, thereby ensuring the uniformity, stability and safety of the product.

[0025] In summary, this application has the following beneficial effects: 1. Because this application uses a mixture of peach blossom Bletilla striata polysaccharide complex, silkworm enzymatic hydrolysis oligopeptides, biomimetic membrane-modified starch microspheres, winter melon juice lactic acid bacteria fermentation supernatant, and antioxidant complex in proportion to weight, the complex and oligopeptides exist in a molecular-level dispersion state, the microspheres act as carriers to uniformly carry the active components, the fermentation broth makes each component form a fine mud-like paste, and the antioxidant complex maintains the stability of the system, thus achieving the effect of highly dispersed active ingredients, full contact with the skin during application, and improved retention rate of active substances after washing. This solves the problem of existing masks, which make it difficult to fully release active ingredients into the liquid phase, easily leading to low absorption and utilization rate of effective ingredients by the skin, and most of the active substances are carried away during washing, resulting in a limited proportion of actual effects.

[0026] 2. This application employs a three-layer structure microsphere consisting of a porous starch core, a biomimetic lipid membrane, and a chitosan oligosaccharide hydrophilic layer, arranged sequentially from the inside out. The biomimetic lipid membrane can fuse with stratum corneum lipids to promote penetration, the chitosan oligosaccharide hydrophilic layer carries a positive charge to achieve electrostatic adsorption and anchoring on the skin surface, and the porous starch core enables the slow release of active ingredients. Simultaneously, the pH of winter melon juice after lactic acid bacteria fermentation is 4.0-4.5, and its organic acids can temporarily increase the permeability of the stratum corneum and provide an optimal protonation environment for chitosan oligosaccharides. The microspheres and fermentation broth form a chain-like synergy, namely, the fermentation broth loosens the stratum corneum, the microspheres anchor and penetrate, and the microspheres slowly release active ingredients, thus continuing to release them even after washing, improving transdermal absorption efficiency and long-lasting moisturizing ability.

[0027] 3. This application utilizes the flavonoid components in the Bletilla striata polysaccharide complex to inhibit tyrosinase activity, the enzymatic hydrolysis of oligopeptides by Bombyx mori to promote the metabolism of melanin-containing keratinocytes, and the biomimetic membrane microspheres to target and deliver the active ingredients to the hair follicle opening and the basal layer of the epidermis for continuous release, synergistically achieving a comprehensive whitening and spot-removing effect. In addition, the antioxidant complex formed by the inclusion of tea extract, rosemary extract and vitamin E succinate with cyclodextrin can scavenge free radicals and form an antioxidant microenvironment on the surface of the microspheres, protecting the flavonoids and polysaccharides from oxidation, so that the product maintains stable efficacy at room temperature without the need to add chemical preservatives. Attached Figure Description

[0028] Figure 1 This is a flowchart of a method for preparing a facial mask with whitening, moisturizing and spot-removing functions provided in this application. Detailed Implementation

[0029] The present application will be further described in detail below with reference to the accompanying drawings and embodiments.

[0030] Technical concept: Existing powder masks typically use raw medicinal materials such as peach blossoms, Bletilla striata, and Bombyx mori as raw powders, which consumers mix with water or juice before use. However, the active ingredients in the raw medicinal powders are encapsulated by the plant cell walls, and simple liquid-phase mixing is insufficient to fully release the active ingredients. This results in low absorption and utilization rates of the active ingredients by the skin, with most of the active substances being washed away, limiting the actual whitening and moisturizing effects. At the same time, the lack of a carrier structure to prolong the action time of the active ingredients means that the mask cannot continue to exert its effects after application and washing off.

[0031] Based on this, this application improves the dispersibility and stability of flavonoids in aqueous phase by forming molecular-level complexes with flavonoids from peach blossoms and polysaccharides from Bletilla striata through hydrogen bonds. The complexes are then co-loaded with enzymatically hydrolyzed oligopeptides from Bombyx mori into modified starch microspheres modified with a biomimetic membrane. The three-layer structure of the microspheres—a biomimetic lipid membrane layer to promote stratum corneum penetration, a hydrophilic chitosan oligosaccharide layer for electrostatic adsorption and anchoring on the skin surface, and a porous starch core for slow release of active ingredients—ensures that the active ingredients continue to exert their effects after washing. Furthermore, winter melon juice fermented with lactic acid bacteria is used as the liquid medium, and the resulting organic acids can temporarily... By increasing the permeability of the stratum corneum in a timely manner, favorable conditions are created for the penetration of microspheres. Thus, by compounding peach blossom and Bletilla striata polysaccharide complex, silkworm enzymatic hydrolysis oligopeptide, biomimetic membrane modified starch microspheres, winter melon juice lactic acid bacteria fermentation supernatant, and antioxidant complex in proportion by weight, a mask product with highly dispersed active ingredients, full contact with the skin during application, and improved retention rate of active substances after washing is obtained. This successfully solves the problem of existing masks that are difficult to fully release active ingredients into the liquid phase, which easily leads to low absorption and utilization rate of effective ingredients by the skin, and most of the active substances are taken away during washing, resulting in a limited proportion of actual effects.

[0032] Unless otherwise specified, all experimental methods used below are conventional methods. All materials, reagents, methods, and instruments used, unless otherwise specified, are conventional materials, reagents, methods, and instruments in this field, which can be obtained commercially or prepared according to literature methods by those skilled in the art.

[0033] Preparation Example 1: Preparation of Peach Blossom and Bletilla striata Polysaccharide Complex Preparation of peach blossom extract: Take 100g of dried peach blossoms, crush them through a 40-mesh sieve, add 1250mL of deionized water, and put them into a subcritical water extraction device; set the extraction temperature to 130℃, the pressure to 3MPa, and the extraction time to 45 minutes; after extraction, cool to room temperature, centrifuge the extract at 8000rpm for 15 minutes, and collect the supernatant; concentrate the supernatant under reduced pressure at 58℃ to a solid content of 25% to obtain concentrated peach blossom extract.

[0034] Preparation of Bletilla striata polysaccharide extract: Take 100g of dried Bletilla striata, pulverize it through a 40-mesh sieve, add 2500mL of deionized water, and adjust the pH to 5.25 with 0.1mol / L hydrochloric acid; add a complex enzyme of cellulase and pectinase, with the enzyme addition amount being 0.8% of the substrate mass, and the enzyme activity ratio of cellulase to pectinase being 1:1; stir and hydrolyze in a 50℃ water bath for 2.5 hours; after the enzymatic hydrolysis is completed, heat to 90℃ and hold for 10 minutes to inactivate the enzyme; after cooling, centrifuge at 8000rpm for 15 minutes and collect the supernatant; concentrate the supernatant under reduced pressure at 58℃ to a solid content of 18%; add anhydrous ethanol to the concentrate to a final ethanol concentration of 70%, and let stand overnight at 4℃; centrifuge at 8000rpm for 15 minutes to collect the precipitate, wash twice with anhydrous ethanol, and vacuum dry at 40℃ for 12 hours to obtain Bletilla striata polysaccharide extract powder with a mass of 18.5g.

[0035] Complexation reaction: Take 20g of peach blossom extract concentrate and add 8g of Bletilla striata polysaccharide extract powder at a dry matter weight ratio of 1:2.5. Adjust the pH to 5.25 with 0.1mol / L citric acid. Stir the reaction in a 50℃ water bath for 2.5 hours. After the reaction is completed, spray dry the reaction solution with an inlet air temperature of 170℃, an outlet air temperature of 85℃, and an atomizer speed of 28000rpm to obtain 26.5g of peach blossom Bletilla striata polysaccharide complex powder.

[0036] Preparation Example 2: Preparation of Oligopeptides from Enzymatic Hydrolysis of Silkworm (Bombyx mori) Take 100g of dried silkworm pupae, pulverize and pass through a 40-mesh sieve, add 1250mL of deionized water, and adjust the pH to 7.25 with 0.1mol / L sodium hydroxide solution; add 0.8g of neutral protease, and stir and hydrolyze in a water bath at 52.5℃ for 3.5 hours; after hydrolysis, heat to 90℃ and hold for 15 minutes to inactivate the enzyme; after cooling, centrifuge at 8000rpm for 20 minutes and collect the supernatant; pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000Da at an operating pressure of 0.25MPa and collect the permeate; concentrate the permeate under reduced pressure at 58℃ to a solid content of 12%, and spray dry at an inlet air temperature of 170℃, an outlet air temperature of 85℃, and an atomizer speed of 28000rpm to obtain 8.2g of silkworm pupae enzymatic hydrolyzed oligopeptide powder.

[0037] Preparation Example 3: Preparation of modified starch microspheres with biomimetic membrane modification Preparation of porous starch core: 20g of glutinous corn starch was weighed and added to deionized water to prepare a 12% (w / v) starch slurry. The slurry was stirred and gelatinized in a 93℃ water bath for 30 minutes and then cooled to room temperature for later use. 3g of Span 80 and 2g of Tween 80 were weighed and dissolved in 200mL of soybean oil to prepare the oil phase. The gelatinized starch solution was slowly added dropwise to the oil phase and emulsified at 10000rpm for 5 minutes to form a W / O type emulsion. 0.15g of epichlorohydrin was added to the emulsion and the crosslinking reaction was stirred in a 45℃ water bath for 5 hours. After the reaction, the mixture was centrifuged at 8000rpm for 10 minutes. The precipitate was washed twice with anhydrous ethanol and twice with deionized water, and then vacuum dried at 40℃ for 12 hours to obtain 16.8g of porous starch core powder.

[0038] Active ingredient loading: 14g of the peach blossom Bletilla striata polysaccharide complex powder prepared in Preparation Example 1 and 4g of the silkworm enzymatic hydrolysis oligopeptide powder prepared in Preparation Example 2 were mixed at a weight ratio of 3.5:1 and dissolved in a phosphate buffer solution at pH 5.75 to prepare a 12.5% ​​solution. The phosphate buffer solution was a 0.02mol / L sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution. 10g of starch porous cores were added to the above 12.5% ​​solution, with a microsphere to solution mass ratio of 1:7.5. The mixture was placed in a vacuum desiccator and evacuated to a vacuum degree of 0.085MPa for 30 minutes. After restoring to normal pressure, the mixture was centrifuged at 8000rpm for 10 minutes, and the precipitate was collected. The precipitate was vacuum dried at 40℃ for 8 hours to obtain 10.2g of microspheres loaded with active ingredients.

[0039] Biomimetic lipid membrane coating: 4.5g of soybean lecithin and 0.7g of cholesterol were weighed and dissolved in 100mL of anhydrous ethanol to prepare a 5.2% (w / v) lipid solution. 10g of microspheres loaded with active ingredients were added to the lipid solution and stirred in a 42℃ water bath for 30 minutes. The mixture was transferred to a rotary evaporator and the ethanol was removed by rotary evaporation at 42℃ and a vacuum of 0.085MPa. The microspheres were collected and dried under vacuum at 40℃ for 8 hours to obtain 10.8g of biomimetic lipid membrane-coated microspheres.

[0040] Spraying of the hydrophilic layer of chitosan oligosaccharide: Weigh 3g of chitosan oligosaccharide with a molecular weight of 2000 Da, dissolve it in 100mL of deionized water to prepare a 3% solution, adjust the pH to 5.25 with 0.1mol / L citric acid to obtain the chitosan oligosaccharide coating solution; Place 10g of biomimetic lipid membrane-coated microspheres in a fluidized bed coating machine, using bottom spraying, with an inlet air temperature of 42℃, a material temperature of 38℃, an atomization pressure of 0.18MPa, a spraying rate of 1.5mL / min, and a coating weight gain of 8%; After coating, fluidize and dry at 40℃ for 15 minutes to obtain 10.6g of biomimetic membrane-modified starch microspheres.

[0041] Preparation Example 4: Preparation of Supernatant from Lactic Acid Bacteria Fermentation of Winter Melon Juice Take 1000g of fresh winter melon, peel and remove seeds, cut into 1.5cm pieces, and put into a juicer to extract juice. Filter the juice through double-layered gauze to remove coarse fibers, obtaining 850mL of original winter melon juice; add 63.8g of 7.5% glucose to the original winter melon juice, stir to dissolve, and adjust the pH to 6.25 with 0.1mol / L sodium hydroxide solution; pour the adjusted winter melon juice into Erlenmeyer flasks, autoclave at 121℃ for 15 minutes, and cool to 37℃.

[0042] Lactobacillus plantarum and Streptococcus thermophilus lyophilized powders were inoculated separately into MRS liquid medium and activated at 37°C for 24 hours to obtain activated bacterial solutions with a viable count ≥10⁻⁶. 8 CFU / mL; the activated Lactobacillus plantarum and Streptococcus thermophilus bacterial solutions were mixed at a volume ratio of 1:1 and inoculated into the sterilized winter melon juice at a total inoculation amount of 1.5% (v / v); the mixture was placed in a constant temperature incubator at 37℃ for 30 hours for static fermentation, and the pH was 4.25 at the end of fermentation.

[0043] After fermentation, the fermentation broth was centrifuged at 8000 rpm for 15 minutes to remove the bacterial precipitate and collect the supernatant. The supernatant was filtered through a 0.22 μm polyethersulfone filter membrane to remove bacteria, yielding 780 mL of winter melon juice lactic acid bacteria fermentation supernatant.

[0044] Preparation Example 5: Preparation of Antioxidant Complex Weigh 3g of tea extract, 1g of rosemary extract, and 1g of vitamin E succinate, mix them evenly to obtain 5g of antioxidant active ingredient mixture; weigh 25g of hydroxypropyl-β-cyclodextrin, add it to 100mL of deionized water to prepare a 25% (w / v) solution, and stir to dissolve it in a 55℃ water bath; add the antioxidant active ingredient mixture to the hydroxypropyl-β-cyclodextrin solution, and stir to encapsulate it in a 55℃ water bath for 2.5 hours; after encapsulation, spray dry the encapsulation solution with an inlet air temperature of 170℃, an outlet air temperature of 85℃, and an atomizer speed of 28000rpm to obtain 27.5g of antioxidant complex powder.

[0045] Preparation of the face mask: 20g of the peach blossom Bletilla striata polysaccharide complex powder obtained in Preparation Example 1, 8.5g of the silkworm pupae enzymatic hydrolysate oligopeptide powder obtained in Preparation Example 2, 15g of the biomimetic membrane-modified starch microspheres obtained in Preparation Example 3, and 2g of the antioxidant complex powder obtained in Preparation Example 5 were added to a three-dimensional mixer under aseptic conditions and mixed at 150 rpm for 12.5 minutes to obtain 45.5g of mixed powder. 42.5g of the winter melon juice lactic acid bacteria fermentation supernatant obtained in Preparation Example 4 was added to a vacuum homogenizer and emulsifier and stirred at 75 rpm. While stirring, the above mixed powder was slowly added. After the addition was complete, the vacuum was drawn to 0.07 MPa, and homogenized at 4000 rpm for 7.5 minutes to form a uniform and fine mud-like paste. The paste was filled into clean packaging containers, sealed, and then... 60 Sterilization by Co-γ irradiation with a dose of 7.5 kGy yields the final product, a facial mask.

[0046] To better understand the above technical solutions, the technical solutions of the present invention will be clearly and completely described below in conjunction with embodiments.

[0047] The following is a further description with reference to the embodiments: Example 1: Please refer to the appendix Figure 1 A facial mask with whitening, moisturizing and spot-removing functions includes the following components in parts by weight: 20 parts of peach blossom Bletilla striata polysaccharide complex, 8.5 parts of silkworm enzymatic hydrolysis oligopeptide, 15 parts of biomimetic membrane modified starch microspheres, 42.5 parts of winter melon juice lactic acid bacteria fermentation supernatant, and 2 parts of antioxidant complex.

[0048] The peach blossom and Bletilla striata polysaccharide complex is formed by complexing peach blossom extract and Bletilla striata polysaccharide extract at a dry matter weight ratio of 1:2.5. The peach blossom extract is obtained by subcritical water extraction of peach blossoms, and the Bletilla striata polysaccharide extract is obtained by enzyme-assisted water extraction of Bletilla striata.

[0049] The modified starch microspheres modified with biomimetic membrane consist of, from the inside out, a porous starch core, a biomimetic lipid membrane layer coating the surface of the porous starch core, and a chitosan oligosaccharide hydrophilic layer sprayed onto the surface of the biomimetic lipid membrane layer. The porous starch core has a particle size of 35 micrometers and a porosity of 50%. The biomimetic lipid membrane layer is composed of soybean lecithin and cholesterol in a molar ratio of 6.5:1. The thickness of the chitosan oligosaccharide hydrophilic layer is 30 nm, and the molecular weight of the chitosan oligosaccharide is 2000 Da.

[0050] The supernatant from the lactic acid bacteria fermentation of winter melon juice was obtained by centrifugation and filtration after the original winter melon juice underwent mixed fermentation with Lactobacillus plantarum and Streptococcus thermophilus. The pH of the fermentation supernatant was 4.25. The antioxidant complex was composed of tea extract, rosemary extract, and vitamin E succinate mixed in a weight ratio of 3:1:1 and then encapsulated with hydroxypropyl-β-cyclodextrin. The average particle size of the antioxidant complex was 200 nm.

[0051] A method for preparing a facial mask with whitening, moisturizing, and blemish-removing functions, applicable to the aforementioned facial mask with whitening, moisturizing, and blemish-removing functions, includes the following steps: S1. Peach blossom Bletilla striata polysaccharide complex, white silkworm enzymatic hydrolysis oligopeptide and biomimetic membrane modified starch microspheres were prepared sequentially. The preparation of the peach blossom and Bletilla striata polysaccharide complex includes the following steps: dried peach blossoms are taken, and deionized water is added at a material-to-liquid ratio of 1:12.5 g / mL. The mixture is extracted with subcritical water at 130℃ and 3MPa for 45 minutes, centrifuged, and concentrated to obtain a concentrated peach blossom extract. Dried Bletilla striata is taken, and deionized water is added at a material-to-liquid ratio of 1:25 g / mL. The pH is adjusted to 5.25, and a complex enzyme of cellulase and pectinase is added. The mixture is enzymatically hydrolyzed at 50℃ for 2.5 hours, the enzyme is inactivated, centrifuged, concentrated, precipitated with alcohol, and dried to obtain a Bletilla striata polysaccharide extract. The concentrated peach blossom extract and the Bletilla striata polysaccharide extract are mixed at a dry matter weight ratio of 1:2.5, the pH is adjusted to 5.25, and the mixture is reacted at 50℃ for 2.5 hours. The mixture is then spray-dried to obtain the peach blossom and Bletilla striata polysaccharide complex. The preparation steps of enzymatic hydrolysis oligopeptides of white silkworm are as follows: take dried white silkworm, add deionized water at a material-to-liquid ratio of 1:12.5 g / mL, adjust the pH to 7.25, add neutral protease, enzymatically hydrolyze at 52.5℃ for 3.5 hours, inactivate the enzyme, centrifuge, collect the supernatant, pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, collect the permeate, concentrate, spray dry, and obtain enzymatic hydrolysis oligopeptides of white silkworm; The preparation of biomimetic membrane-modified starch microspheres includes the following steps: glutinous corn starch is gelatinized and emulsified with an oil phase, crosslinked with epichlorohydrin, washed, and dried to obtain a porous starch core with a particle size of 35 micrometers and a porosity of 50%; soybean lecithin and cholesterol are mixed at a molar ratio of 6.5:1, dissolved in anhydrous ethanol, and the porous starch core is added, stirred, the solvent is removed, and dried to obtain biomimetic lipid membrane-coated microspheres; the biomimetic lipid membrane-coated microspheres are placed in a fluidized bed, sprayed with chitosan oligosaccharide solution, and dried to obtain biomimetic membrane-modified starch microspheres.

[0052] S2. Peach blossom Bletilla striata polysaccharide complex and silkworm pupa enzymatic hydrolysis oligopeptide were loaded onto biomimetic membrane modified starch microspheres to obtain microspheres loaded with active ingredients. The loading process includes the following steps: A polysaccharide complex of *Bletilla striata* and enzymatically hydrolyzed oligopeptides of *Bombyx mori* are mixed at a weight ratio of 3.5:1 and dissolved in a phosphate buffer solution at pH 5.75 to prepare a 12.5% ​​solution; modified starch microspheres modified with a biomimetic membrane are added to the solution at a mass ratio of 1:7.5; after immersion in the solution under a vacuum of 0.085 MPa for 30 minutes, the microspheres are collected by centrifugation and then vacuum-dried at 40°C for 8 hours to obtain microspheres loaded with active ingredients.

[0053] S3. Prepare the supernatant of winter melon juice lactic acid bacteria fermentation and the antioxidant complex in sequence; The preparation of the supernatant from the lactic acid bacteria fermentation of winter melon juice includes the following steps: Take fresh winter melon, peel and remove seeds, juice it, filter it to obtain winter melon juice; add 7.5% glucose to the winter melon juice, adjust the pH to 6.25, and sterilize it; inoculate with Lactobacillus plantarum and Streptococcus thermophilus, with a total inoculation amount of 1.5%, and ferment at 37℃ for 30 hours until the pH drops to 4.25; centrifuge, collect the supernatant, filter and sterilize it to obtain the supernatant from the lactic acid bacteria fermentation of winter melon juice.

[0054] S4. Mix the microspheres loaded with active ingredients and the antioxidant complex evenly, add the supernatant of winter melon juice lactic acid bacteria fermentation, homogenize, fill, sterilize, and obtain the face mask.

[0055] The mixing process involves mixing microspheres loaded with active ingredients and an antioxidant complex at 150 rpm for 12.5 minutes to form a mixed powder. Homogenization involves adding the supernatant from the winter melon juice lactic acid bacteria fermentation to a vacuum homogenizer, stirring at 75 rpm, adding the mixed powder, vacuuming to 0.07 MPa, and homogenizing at 4000 rpm for 7.5 minutes to form a paste-like consistency. Sterilization is performed using… 60 Sterilization by Co-γ irradiation, with an irradiation dose of 7.5 kGy.

[0056] Example 2: This example differs from Example 1 above in that: A facial mask with whitening, moisturizing and spot-removing functions comprises the following components in parts by weight: 25 parts of peach blossom Bletilla striata polysaccharide complex, 12 parts of silkworm enzymatic hydrolysis oligopeptide, 20 parts of biomimetic membrane modified starch microspheres, 50 parts of winter melon juice lactic acid bacteria fermentation supernatant, and 3 parts of antioxidant complex.

[0057] The peach blossom and Bletilla striata polysaccharide complex is formed by complexing peach blossom extract and Bletilla striata polysaccharide extract at a dry matter weight ratio of 1:3. The peach blossom extract is obtained by subcritical water extraction of peach blossoms, and the Bletilla striata polysaccharide extract is obtained by enzyme-assisted water extraction of Bletilla striata.

[0058] The modified starch microspheres modified with biomimetic membrane consist of, from the inside out, a porous starch core, a biomimetic lipid membrane layer coating the surface of the porous starch core, and a chitosan oligosaccharide hydrophilic layer sprayed onto the surface of the biomimetic lipid membrane layer. The porous starch core has a particle size of 50 micrometers and a porosity of 60%. The biomimetic lipid membrane layer is composed of soybean lecithin and cholesterol in a molar ratio of 8:1. The thickness of the chitosan oligosaccharide hydrophilic layer is 50 nm, and the molecular weight of the chitosan oligosaccharide is 3000 Da.

[0059] The supernatant from the lactic acid bacteria fermentation of winter melon juice was obtained by centrifugation and filtration after the original winter melon juice underwent mixed fermentation with Lactobacillus plantarum and Streptococcus thermophilus. The pH of the fermentation supernatant was 4.5. The antioxidant complex was composed of tea extract, rosemary extract, and vitamin E succinate mixed in a weight ratio of 4:1:1 and then encapsulated with hydroxypropyl-β-cyclodextrin. The average particle size of the antioxidant complex was 300 nm.

[0060] A method for preparing a facial mask with whitening, moisturizing, and blemish-removing functions, applicable to the aforementioned facial mask with whitening, moisturizing, and blemish-removing functions, includes the following steps: S1. Peach blossom Bletilla striata polysaccharide complex, white silkworm enzymatic hydrolysis oligopeptide and biomimetic membrane modified starch microspheres were prepared sequentially. The preparation of the peach blossom and Bletilla striata polysaccharide complex includes the following steps: dried peach blossoms are taken, and deionized water is added at a material-to-liquid ratio of 1:15 g / mL. The mixture is extracted with subcritical water at 140℃ and 4MPa for 60 minutes, centrifuged, and concentrated to obtain a concentrated peach blossom extract. Dried Bletilla striata is taken, and deionized water is added at a material-to-liquid ratio of 1:30 g / mL. The pH is adjusted to 5.5, and a complex enzyme of cellulase and pectinase is added. The mixture is enzymatically hydrolyzed at 55℃ for 3 hours, the enzyme is inactivated, centrifuged, concentrated, precipitated with alcohol, and dried to obtain a Bletilla striata polysaccharide extract. The concentrated peach blossom extract and the Bletilla striata polysaccharide extract are mixed at a dry matter weight ratio of 1:3, the pH is adjusted to 5.5, and the mixture is reacted at 55℃ for 3 hours. The mixture is then spray-dried to obtain the peach blossom and Bletilla striata polysaccharide complex. The preparation steps of enzymatic hydrolysis oligopeptides of white silkworm are as follows: take dried white silkworm, add deionized water at a material-to-liquid ratio of 1:15 g / mL, adjust the pH to 7.5, add neutral protease, enzymatically hydrolyze at 55℃ for 4 hours, inactivate the enzyme, centrifuge, collect the supernatant, pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, collect the permeate, concentrate, spray dry, and obtain enzymatic hydrolysis oligopeptides of white silkworm. The preparation of biomimetic membrane-modified starch microspheres includes the following steps: gelatinizing glutinous corn starch and mixing and emulsifying it with an oil phase, adding epichlorohydrin for crosslinking, washing, and drying to obtain a porous starch core with a particle size of 50 micrometers and a porosity of 60%; mixing soybean lecithin and cholesterol at a molar ratio of 8:1, dissolving in anhydrous ethanol, adding the porous starch core, stirring, removing the solvent, and drying to obtain biomimetic lipid membrane-coated microspheres; placing the biomimetic lipid membrane-coated microspheres in a fluidized bed, spraying with chitosan oligosaccharide solution, and drying to obtain biomimetic membrane-modified starch microspheres.

[0061] S2. Peach blossom Bletilla striata polysaccharide complex and silkworm pupa enzymatic hydrolysis oligopeptide were loaded onto biomimetic membrane modified starch microspheres to obtain microspheres loaded with active ingredients. The loading process includes the following steps: A polysaccharide complex of *Bletilla striata* and enzymatically hydrolyzed oligopeptides of *Bombyx mori* are mixed at a weight ratio of 4:1 and dissolved in a phosphate buffer solution at pH 6.0 to prepare a 15% solution; modified starch microspheres modified with a biomimetic membrane are added to the solution at a mass ratio of 1:10; the microspheres are immersed in the solution under a vacuum of 0.09 MPa for 35 minutes, centrifuged to collect the microspheres, and then vacuum-dried at 45°C for 9 hours to obtain microspheres loaded with active ingredients.

[0062] S3. Prepare the supernatant of winter melon juice lactic acid bacteria fermentation and the antioxidant complex in sequence; The preparation of the supernatant from the lactic acid bacteria fermentation of winter melon juice includes the following steps: take fresh winter melon, peel and remove seeds, juice it, filter it to obtain winter melon juice; add 10% glucose to the winter melon juice, adjust the pH to 6.5, and sterilize it; inoculate with Lactobacillus plantarum and Streptococcus thermophilus, with a total inoculation amount of 2%, and ferment at 37℃ for 36 hours until the pH drops to 4.5; centrifuge, collect the supernatant, filter and sterilize it to obtain the supernatant from the lactic acid bacteria fermentation of winter melon juice.

[0063] S4. Mix the microspheres loaded with active ingredients and the antioxidant complex evenly, add the supernatant of winter melon juice lactic acid bacteria fermentation, homogenize, fill, sterilize, and obtain the face mask.

[0064] The mixing process involves mixing microspheres loaded with active ingredients with an antioxidant complex at 200 rpm for 15 minutes to form a mixed powder. Homogenization involves adding the supernatant from the winter melon juice lactic acid bacteria fermentation to a vacuum homogenizer, stirring at 100 rpm, adding the mixed powder, vacuuming to 0.08 MPa, and homogenizing at 5000 rpm for 10 minutes to form a paste-like consistency. Sterilization is performed using… 60 Sterilization by Co-γ irradiation, with an irradiation dose of 10 kGy.

[0065] Example 3: This example differs from Example 1 above in that: A facial mask with whitening, moisturizing and spot-removing functions comprises the following components in parts by weight: 15 parts of peach blossom Bletilla striata polysaccharide complex, 5 parts of silkworm enzymatic hydrolysis oligopeptide, 10 parts of biomimetic membrane modified starch microspheres, 35 parts of winter melon juice lactic acid bacteria fermentation supernatant, and 1 part of antioxidant complex.

[0066] The peach blossom and Bletilla striata polysaccharide complex is formed by complexing peach blossom extract and Bletilla striata polysaccharide extract at a dry matter weight ratio of 1:2. The peach blossom extract is an extract obtained by subcritical water extraction of peach blossoms, and the Bletilla striata polysaccharide extract is a polysaccharide extract obtained by enzyme-assisted water extraction of Bletilla striata.

[0067] The modified starch microspheres modified with biomimetic membrane consist of, from the inside out, a porous starch core, a biomimetic lipid membrane layer coating the surface of the porous starch core, and a chitosan oligosaccharide hydrophilic layer sprayed onto the surface of the biomimetic lipid membrane layer. The porous starch core has a particle size of 20 micrometers and a porosity of 40%. The biomimetic lipid membrane layer is composed of soybean lecithin and cholesterol in a molar ratio of 5:1. The thickness of the chitosan oligosaccharide hydrophilic layer is 10 nm, and the molecular weight of the chitosan oligosaccharide is 1000 Da.

[0068] The supernatant from the lactic acid bacteria fermentation of winter melon juice was obtained by centrifugation and filtration after the original winter melon juice underwent mixed fermentation with Lactobacillus plantarum and Streptococcus thermophilus. The pH of the fermentation supernatant was 4.0. The antioxidant complex was composed of tea extract, rosemary extract, and vitamin E succinate mixed in a weight ratio of 2:1:1 and then encapsulated with hydroxypropyl-β-cyclodextrin. The average particle size of the antioxidant complex was 100 nm.

[0069] A method for preparing a facial mask with whitening, moisturizing, and blemish-removing functions, applicable to the aforementioned facial mask with whitening, moisturizing, and blemish-removing functions, includes the following steps: S1. Peach blossom Bletilla striata polysaccharide complex, white silkworm enzymatic hydrolysis oligopeptide and biomimetic membrane modified starch microspheres were prepared sequentially. The preparation of the peach blossom and Bletilla striata polysaccharide complex includes the following steps: dried peach blossoms are taken, and deionized water is added at a material-to-liquid ratio of 1:10 g / mL. The mixture is extracted with subcritical water at 120℃ and 2MPa for 30 minutes, centrifuged, and concentrated to obtain a concentrated peach blossom extract. Dried Bletilla striata is taken, and deionized water is added at a material-to-liquid ratio of 1:20 g / mL. The pH is adjusted to 5.0, and a complex enzyme of cellulase and pectinase is added. The mixture is enzymatically hydrolyzed at 45℃ for 2 hours, the enzyme is inactivated, centrifuged, concentrated, precipitated with alcohol, and dried to obtain a Bletilla striata polysaccharide extract. The concentrated peach blossom extract and the Bletilla striata polysaccharide extract are mixed at a dry matter weight ratio of 1:2, the pH is adjusted to 5.0, and the mixture is reacted at 45℃ for 2 hours. The mixture is then spray-dried to obtain the peach blossom and Bletilla striata polysaccharide complex. The preparation steps of enzymatic hydrolysis oligopeptides of white silkworm are as follows: take dried white silkworm, add deionized water at a material-to-liquid ratio of 1:10 g / mL, adjust the pH to 7.0, add neutral protease, enzymatically hydrolyze at 50℃ for 3 hours, inactivate the enzyme, centrifuge, collect the supernatant, pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, collect the permeate, concentrate, spray dry, and obtain enzymatic hydrolysis oligopeptides of white silkworm. The preparation of biomimetic membrane-modified starch microspheres includes the following steps: gelatinizing glutinous corn starch and mixing and emulsifying it with an oil phase, adding epichlorohydrin for crosslinking, washing, and drying to obtain a porous starch core with a particle size of 20 micrometers and a porosity of 40%; mixing soybean lecithin and cholesterol at a molar ratio of 5:1, dissolving in anhydrous ethanol, adding the porous starch core, stirring, removing the solvent, and drying to obtain biomimetic lipid membrane-coated microspheres; placing the biomimetic lipid membrane-coated microspheres in a fluidized bed, spraying with chitosan oligosaccharide solution, and drying to obtain biomimetic membrane-modified starch microspheres.

[0070] S2. Peach blossom Bletilla striata polysaccharide complex and silkworm pupa enzymatic hydrolysis oligopeptide were loaded onto biomimetic membrane modified starch microspheres to obtain microspheres loaded with active ingredients. The loading process includes the following steps: A polysaccharide complex of *Bletilla striata* and enzymatically hydrolyzed oligopeptides of *Bombyx mori* are mixed at a weight ratio of 3:1 and dissolved in a phosphate buffer solution at pH 5.5 to prepare a 10% solution; modified starch microspheres modified with a biomimetic membrane are added to the solution at a mass ratio of 1:5; after immersion in the solution under a vacuum of 0.08 MPa for 25 minutes, the microspheres are collected by centrifugation and then vacuum-dried at 35°C for 7 hours to obtain microspheres loaded with active ingredients.

[0071] S3. Prepare the supernatant of winter melon juice lactic acid bacteria fermentation and the antioxidant complex in sequence; The preparation of the supernatant from the lactic acid bacteria fermentation of winter melon juice includes the following steps: take fresh winter melon, peel and remove seeds, juice it, filter it to obtain winter melon juice; add 5% glucose to the winter melon juice, adjust the pH to 6.0, and sterilize it; inoculate with Lactobacillus plantarum and Streptococcus thermophilus, with a total inoculation amount of 1%, and ferment at 37℃ for 24 hours until the pH drops to 4.0; centrifuge, collect the supernatant, filter and sterilize it to obtain the supernatant from the lactic acid bacteria fermentation of winter melon juice.

[0072] S4. Mix the microspheres loaded with active ingredients and the antioxidant complex evenly, add the supernatant of winter melon juice lactic acid bacteria fermentation, homogenize, fill, sterilize, and obtain the face mask.

[0073] The mixing process involves mixing the microspheres loaded with active ingredients and the antioxidant complex at 100 rpm for 10 minutes to form a mixed powder. Homogenization involves adding the supernatant from the winter melon juice lactic acid bacteria fermentation to a vacuum homogenizer, stirring at 50 rpm, adding the mixed powder, vacuuming to 0.06-0.08 MPa, and homogenizing at 3000 rpm for 5 minutes to form a paste-like consistency. Sterilization is performed using… 60 Sterilization by Co-γ irradiation, with an irradiation dose of 5 kGy.

[0074] Comparative Example 1: A powdered facial mask with whitening, moisturizing and spot-removing functions, including the following raw materials: 3 liang of peach blossom, 5 liang of winter melon, 1 liang of white silkworm, 2 liang of talc, and 1 liang of Bletilla striata.

[0075] Preparation method: Take the above-mentioned peach blossoms, white silkworm, talc, and Bletilla striata, wash them separately, and dry them to constant weight; pulverize each of the dried medicinal materials separately using a pulverizer, pass them through a 200-mesh sieve, and obtain fine powder of each raw material; place the fine powder of each medicinal material in a mixer according to the proportion, mix them thoroughly and evenly to obtain compound powder; take fresh winter melon, peel and remove seeds, juice it, filter it, and obtain winter melon juice; mix the above compound powder and winter melon juice at a volume ratio of 3:1, stir thoroughly to form a paste, and obtain a powdered face mask.

[0076] Comparative Example 2: This comparative example differs from Example 1 above in that: The modified starch microspheres modified with biomimetic membranes were replaced with unmodified starch microspheres. The specific preparation method is as follows: After gelatinizing glutinous corn starch, it was mixed and emulsified with the oil phase, and then crosslinked with epichlorohydrin. After washing and drying, a porous starch core was obtained with a particle size of 35 micrometers and a porosity of 50%. The polysaccharide complex of Bletilla striata and the enzymatically hydrolyzed oligopeptide of Bombyx mori were mixed at a weight ratio of 3.5:1 and dissolved in phosphate buffer at pH 5.75 to prepare a 12.5% ​​solution. The porous starch core was added to the solution, with a microsphere to solution mass ratio of 1:7.5. The mixture was immersed in the solution under a vacuum of 0.085 MPa for 30 minutes, centrifuged to collect the microspheres, and then vacuum-dried at 40°C for 8 hours to obtain microspheres loaded with the active ingredient. Other steps were the same as in Example 1.

[0077] Comparative Example 3: This comparative example differs from Example 1 above in that: The supernatant from the lactic acid bacteria fermentation of winter melon juice was replaced with unfermented winter melon juice, i.e., raw winter melon juice obtained by juicing and filtering fresh winter melon. Everything else was the same as in Example 1.

[0078] Comparative Example 4: This comparative example differs from Example 1 above in that: The chitosan oligosaccharide in the hydrophilic layer has a molecular weight of 500 Da, and the specific preparation method is as follows: Chitosan oligosaccharide with a molecular weight of 500 Da was dissolved in deionized water to prepare a 3% solution, and the pH was adjusted to 5.25 to obtain a chitosan oligosaccharide coating solution. Biomimetic lipid membrane-coated microspheres were placed in a fluidized bed coating machine using a bottom spray method. The inlet air temperature was 42℃, the material temperature was 38℃, the atomization pressure was 0.18MPa, the spray rate was 1.5mL / min, and the coating weight gain was 8%. After coating, the microspheres were fluidized and dried at 40°C for 15 minutes to obtain biomimetic membrane-modified starch microspheres.

[0079] Everything else is the same as in Example 1.

[0080] Comparative Example 5: This comparative example differs from Example 1 above in that: The fermentation endpoint pH of the supernatant from the lactic acid bacteria fermentation of winter melon juice was 3.5. The specific preparation method is as follows: Take a fresh winter melon, peel and remove the seeds, juice it, filter it, and obtain the original winter melon juice; Add 7.5% glucose to the original winter melon juice, adjust the pH to 6.25, and sterilize. The mixture was inoculated with Lactobacillus plantarum and Streptococcus thermophilus at a total inoculum of 1.5%. Fermentation was carried out at 37°C until the pH dropped to 3.5. The mixture was then centrifuged, the supernatant was collected, filtered to remove bacteria, and the resulting supernatant from the fermentation of winter melon juice with lactic acid bacteria was obtained.

[0081] Everything else is the same as in Example 1.

[0082] Performance testing: The mask samples prepared in Examples 1-3 and Comparative Examples 1-5 were subjected to the following tests: Transdermal absorption rate determination: The Franz diffusion cell method was used, with isolated pig skin as the transdermal barrier, to determine the 24-hour cumulative transdermal absorption rate (%) of total flavonoids in each sample. The mask sample was evenly coated on the surface of pig skin, and pH 7.4 phosphate buffer was added to the receiving cell. The sample was kept in a constant temperature water bath at 37℃ and samples were taken at 1, 2, 4, 6, 8, 12 and 24 hours. The total flavonoid content was determined by ultraviolet spectrophotometry, and the cumulative transdermal absorption rate was calculated.

[0083] Tyrosinase inhibition rate determination: The dopachrome method was used. 0.5 mL of each sample extract was taken, and 1.5 mL of pH 6.8, 0.1 mol / L phosphate buffer, 0.5 mL of 0.1% tyrosine solution, and 0.5 mL of 200 U / mL tyrosinase solution were added. After mixing, the mixture was incubated in a water bath at 37℃ for 20 minutes, and the absorbance was measured at 475 nm to calculate the tyrosinase inhibition rate.

[0084] Skin moisture content determination: Thirty healthy volunteers aged 25-55 years were randomly divided into 8 groups of 3 people each. A 3cm×3cm area was marked on the inner forearm of the subjects, and the corresponding sample was applied to each area. The amount of sample applied each time was 0.2g. The sample was washed off after 20 minutes. The skin moisture content of the stratum corneum was measured before application, and at 1 hour, 4 hours and 8 hours after application using a skin moisture meter. The rate of increase in moisture content was calculated.

[0085] Skin melanin content determination: Thirty healthy volunteers aged 25-55 years were randomly divided into 8 groups of 3 people each. A 3cm×3cm area was marked on the inner forearm of the subjects, and the corresponding sample was used continuously for 4 weeks, 3 times a week. The skin melanin content was measured before use and in the 1st, 2nd and 4th weeks after use using a skin melanin tester, and the melanin content reduction rate was calculated.

[0086] Skin occlusive patch test: Refer to the skin occlusive patch test method in the "Cosmetic Safety Technical Specifications" (2015 edition); 30 healthy volunteers were selected, and the samples of Examples 1-3 and Comparative Examples 1-5 were placed in the patch tester and applied to the back of the subjects. The patch tester was removed after 48 hours, and the skin reaction was observed at 0.5 hours, 24 hours and 48 hours after removal. The skin irritation and sensitization were evaluated according to the grading standard.

[0087] Table 1: Results of transdermal absorption rate and tyrosinase inhibition rate detection ; Table 2: Results of Skin Moisture Content Growth Rate Detection ; Table 3: Results of the test for the decrease rate of skin melanin content ; Table 4: Results of Skin Occlusive Patch Test ; Note: No positive reactions, such as skin irritation symptoms like erythema and edema, were observed in any of the 30 subjects. None of the samples showed skin irritation or sensitization.

[0088] As can be seen from Examples 1 to 3 and Comparative Examples 1 to 5, and Tables 1 to 4, the multi-component synergistic system of this application, composed of peach blossom Bletilla striata polysaccharide complex, silkworm enzymatic hydrolysis oligopeptide, biomimetic membrane-modified starch microspheres, winter melon juice lactic acid bacteria fermentation supernatant, and antioxidant complex, can enhance the transdermal absorption performance and whitening effect of the mask. It improves solubility through molecular-level complexation of active ingredients, achieves targeted delivery through biomimetic membrane-modified microspheres, and promotes stratum corneum permeability through fermentation medium, thereby ensuring the efficient utilization and sustained effect of active ingredients.

[0089] As can be seen from Example 1 and Comparative Example 2, and Table 1, the three-layer structure of the biomimetic membrane-modified starch microspheres can enhance the transdermal absorption of active ingredients. The biomimetic lipid membrane layer can fuse with the lipids of the stratum corneum of the skin to promote penetration, and the chitosan oligosaccharide hydrophilic layer can enhance the electrostatic adsorption of microspheres on the skin surface to achieve anchoring. The synergistic effect of the two ensures the targeted delivery and long-lasting release of active ingredients, while the unmodified microspheres lacking this structure cannot achieve the same delivery efficiency.

[0090] Based on Example 1 and Comparative Example 3, and in conjunction with Table 1, it can be seen that the lactic acid bacteria fermentation treatment of winter melon juice can enhance the penetration effect of the mask. The organic acids produced during the fermentation process can temporarily regulate the permeability of the stratum corneum, creating favorable conditions for the penetration of microspheres. At the same time, the small molecule active substances in the fermentation liquid can help improve the penetration effect of active ingredients, while unfermented winter melon juice lacks the above synergistic effect.

[0091] As can be seen from Example 1 and Comparative Example 4, and Table 1, the molecular weight of chitosan oligosaccharides in the hydrophilic layer can effectively maintain the anchoring effect of microspheres. A suitable molecular weight can provide sufficient positive charge to enhance the electrostatic adsorption with the skin surface. However, if the molecular weight is too small, the adsorption effect weakens, resulting in a decrease in the retention capacity of microspheres on the skin surface, which in turn affects the transdermal absorption efficiency of active ingredients.

[0092] Based on Example 1 and Comparative Example 5, and in conjunction with Table 1, it can be seen that the pH value of the supernatant of winter melon juice lactic acid bacteria fermentation can balance the permeation-enhancing effect and component compatibility. When the pH value is too low, it will affect the protonation state of chitosan oligosaccharides on the surface of microspheres, weaken the electrostatic adsorption effect, and may also have an adverse effect on the stability of active ingredients, thereby affecting the overall transdermal absorption efficiency.

[0093] As can be seen from Examples 1 to 3 and Comparative Example 1, and Table 2, the multi-component combination of this application can enhance the skin's moisturizing and water-locking ability. Through the continuous sustained-release effect of the biomimetic membrane-modified microspheres, the active ingredients can continue to be released after washing. At the same time, the organic acids and amino acids in the fermentation medium can synergistically enhance the water-retention capacity of the stratum corneum, so that the skin's moisture content remains stable for a longer period of time.

[0094] As can be seen from Example 1 and Comparative Example 2, and Table 2, the three-layer structure of the biomimetic membrane-modified starch microspheres is the core factor in maintaining long-lasting skin hydration. The chitosan oligosaccharide hydrophilic layer in this structure can adsorb water and form a moisturizing film on the skin surface, while the biomimetic lipid membrane layer can promote the penetration of active ingredients into the deep stratum corneum. The unmodified microspheres lacking this structure cannot achieve the same long-lasting moisturizing effect.

[0095] Based on Example 1 and Comparative Example 3, and in conjunction with Table 2, it can be seen that the fermentation supernatant obtained after the winter melon juice is fermented with lactic acid bacteria has a synergistic effect on skin moisturizing. The small molecule organic acids, free amino acids and polysaccharides produced by fermentation can jointly enhance the water retention capacity of the stratum corneum. However, the unfermented winter melon juice lacks the above-mentioned active components, and its moisturizing effect is relatively limited.

[0096] As can be seen from Examples 1 to 3 and Comparative Example 1, and in conjunction with Table 3, the multi-component synergistic effect of this application can inhibit the production and deposition of melanin in the skin. By inhibiting tyrosinase activity through the peach blossom Bletilla striata polysaccharide complex, melanin production is reduced from the source. By targeting and delivering whitening ingredients to the hair follicle opening through biomimetic membrane-modified microspheres, and by gently promoting the metabolism of melanin-containing keratinocytes through the organic acids in the fermentation medium, the three pathways work synergistically to achieve skin brightening and fading of pigmentation.

[0097] As can be seen from Example 1 and Comparative Example 2, and Table 3, the three-layer structure of the biomimetic membrane-modified starch microspheres can ensure the continuous effect of whitening ingredients, allowing the active ingredients to be embedded in the hair follicle opening and slowly released, thus prolonging the interaction time between the whitening ingredients and the skin. In contrast, unmodified microspheres lacking this structure cannot achieve the same long-lasting whitening effect.

[0098] Based on Example 1 and Comparative Example 5, and in conjunction with Tables 2 and 3, it can be seen that the pH value of the supernatant of winter melon juice lactic acid bacteria fermentation can affect the moisturizing and whitening effects. Suitable pH conditions can maintain the optimal protonation state of chitosan oligosaccharides on the surface of microspheres, ensuring the electrostatic adsorption and anchoring effect of microspheres. At the same time, the concentration and type of organic acids in the fermentation broth can exert a gentle keratin exfoliation effect under suitable pH conditions, promoting the normal metabolism of melanin-containing keratinocytes.

[0099] As can be seen from Examples 1 to 3 and Comparative Examples 1 to 5, and Table 4, the mask samples of each example and comparative example of this application did not show a positive reaction in the skin occlusive patch test. This indicates that the raw material system used in this application, including peach blossom, Bletilla striata, Bombyx mori, winter melon juice, starch, phospholipids, chitosan oligosaccharide, tea extract, rosemary extract, and vitamin E, has good overall skin safety and meets the safety requirements of the cosmetics and health products fields.

[0100] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A facial mask with whitening, moisturizing, and blemish-removing functions, characterized in that, The product comprises the following components in parts by weight: 15-25 parts of peach blossom Bletilla striata polysaccharide complex, 5-12 parts of silkworm enzymatic hydrolysis oligopeptide, 10-20 parts of biomimetic membrane modified starch microspheres, 35-50 parts of winter melon juice lactic acid bacteria fermentation supernatant, and 1-3 parts of antioxidant complex.

2. A facial mask with whitening, moisturizing, and freckle-removing functions according to claim 1, characterized in that: The peach blossom and Bletilla striata polysaccharide complex is formed by complexing peach blossom extract and Bletilla striata polysaccharide extract at a dry matter weight ratio of 1:2-3. The peach blossom extract is an extract obtained by subcritical water extraction of peach blossoms, and the Bletilla striata polysaccharide extract is a polysaccharide extract obtained by enzyme-assisted water extraction of Bletilla striata.

3. A facial mask with whitening, moisturizing, and freckle-removing functions according to claim 1, characterized in that: The modified starch microspheres modified with the biomimetic membrane consist of, from the inside out, a porous starch core, a biomimetic lipid membrane layer coating the surface of the porous starch core, and a chitosan oligosaccharide hydrophilic layer sprayed onto the surface of the biomimetic lipid membrane layer. The porous starch core has a particle size of 20-50 micrometers and a porosity of 40-60%. The biomimetic lipid membrane layer is composed of soybean lecithin and cholesterol in a molar ratio of 5-8:

1. The thickness of the chitosan oligosaccharide hydrophilic layer is 10-50 nm, and the molecular weight of the chitosan oligosaccharide is 1000-3000 Da.

4. A facial mask with whitening, moisturizing, and freckle-removing functions according to claim 1, characterized in that: The supernatant of the winter melon juice lactic acid bacteria fermentation is the supernatant obtained by centrifugation and filtration after the original winter melon juice is fermented by mixed fermentation of Lactobacillus plantarum and Streptococcus thermophilus. The pH of the fermentation supernatant is 4.0-4.

5.

5. A facial mask with whitening, moisturizing, and freckle-removing functions according to claim 1, characterized in that: The antioxidant complex is composed of tea extract, rosemary extract and vitamin E succinate mixed in a weight ratio of 2-4:1:1 and then encapsulated with hydroxypropyl-β-cyclodextrin. The average particle size of the antioxidant complex is 100-300 nm.

6. A method for preparing a facial mask with whitening, moisturizing, and freckle-removing functions, characterized in that: The application of a facial mask with whitening, moisturizing, and freckle-removing functions as described in any one of claims 1-5 includes the following steps: S1. Peach blossom Bletilla striata polysaccharide complex, white silkworm enzymatic hydrolysis oligopeptide and biomimetic membrane modified starch microspheres were prepared sequentially. S2. Load the peach blossom Bletilla striata polysaccharide complex and the silkworm enzymatic hydrolysis oligopeptide onto the biomimetic membrane-modified starch microspheres to obtain microspheres loaded with active ingredients. S3. Prepare the supernatant of winter melon juice lactic acid bacteria fermentation and the antioxidant complex in sequence; S4. Mix the microspheres loaded with active ingredients with the antioxidant complex evenly, add the supernatant of the winter melon juice lactic acid bacteria fermentation, homogenize, fill, sterilize, and obtain a face mask.

7. The method for preparing a facial mask with whitening, moisturizing, and freckle-removing functions according to claim 6, characterized in that: In step S1, the preparation of the peach blossom Bletilla striata polysaccharide complex includes the following steps: Take dried peach blossoms, add deionized water at a material-to-liquid ratio of 1:10-15 g / mL, and extract in subcritical water at 120-140℃ and 2-4MPa for 30-60 minutes. Centrifuge and concentrate to obtain concentrated peach blossom extract. Take dried Bletilla striata, add deionized water at a material-to-liquid ratio of 1:20-30 g / mL, adjust the pH to 5.0-5.5, add a complex enzyme of cellulase and pectinase, enzymatically hydrolyze at 45-55℃ for 2-3 hours, inactivate the enzyme, centrifuge, concentrate, precipitate with alcohol, and dry to obtain Bletilla striata polysaccharide extract. Peach blossom extract concentrate and Bletilla striata polysaccharide extract were mixed at a dry matter weight ratio of 1:2-3, the pH was adjusted to 5.0-5.5, and the mixture was reacted at 45-55℃ for 2-3 hours. The mixture was then spray-dried to obtain peach blossom Bletilla striata polysaccharide complex. The preparation steps of the enzymatic hydrolysis oligopeptide of *Bombyx mori* are as follows: take dried *Bombyx mori*, add deionized water at a material-to-liquid ratio of 1:10-15 g / mL, adjust the pH to 7.0-7.5, add neutral protease, enzymatically hydrolyze at 50-55℃ for 3-4 hours, inactivate the enzyme, centrifuge, collect the supernatant, pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, collect the permeate, concentrate, spray dry, and obtain enzymatic hydrolysis oligopeptide of *Bombyx mori*. The preparation of the biomimetic membrane-modified starch microspheres includes the following steps: After gelatinizing glutinous corn starch, it is mixed and emulsified with an oil phase, crosslinked with epichlorohydrin, washed, and dried to obtain a porous starch core. The porous starch core has a particle size of 20-50 micrometers and a porosity of 40-60%. Soybean lecithin and cholesterol were mixed at a molar ratio of 5-8:1, dissolved in anhydrous ethanol, and the starch porous core was added. The mixture was stirred, the solvent was removed, and the mixture was dried to obtain biomimetic lipid membrane-coated microspheres. The biomimetic lipid membrane-coated microspheres were placed in a fluidized bed, sprayed with chitosan oligosaccharide solution, and dried to obtain biomimetic membrane-modified starch microspheres.

8. The method for preparing a facial mask with whitening, moisturizing, and freckle-removing functions according to claim 6, characterized in that: In step S2, the load includes the following steps: The polysaccharide complex of Bletilla striata and the enzymatically hydrolyzed oligopeptide of Bombyx mori were mixed at a weight ratio of 3-4:1 and dissolved in phosphate buffer solution at pH 5.5-6.0 to prepare a 10-15% solution. Modified starch microspheres with biomimetic membrane were added to the solution at a mass ratio of 1:5-10. After immersion in the solution under a vacuum of 0.08-0.09 MPa for 25-35 minutes, the microspheres were collected by centrifugation and then vacuum dried at 35-45°C for 7-9 hours to obtain microspheres loaded with active ingredients.

9. The method for preparing a facial mask with whitening, moisturizing, and freckle-removing functions according to claim 6, characterized in that: In step S3, the preparation of the supernatant from the lactic acid bacteria fermentation of winter melon juice includes the following steps: Take a fresh winter melon, peel and remove the seeds, juice it, filter it, and obtain the original winter melon juice; Add 5-10% glucose to the original winter melon juice, adjust the pH to 6.0-6.5, and sterilize. Inoculate with Lactobacillus plantarum and Streptococcus thermophilus, with a total inoculum of 1-2%, and ferment at 37°C for 24-36 hours until the pH drops to 4.0-4.5; Centrifuge, collect the supernatant, filter and sterilize to obtain the supernatant from the lactic acid bacteria fermentation of winter melon juice.

10. A method for preparing a facial mask with whitening, moisturizing, and freckle-removing functions according to claim 6, characterized in that: In step S4, the mixing process involves mixing the microspheres loaded with the active ingredients and the antioxidant complex at 100-200 rpm for 10-15 minutes to form a mixed powder. The homogenization process involves adding the supernatant from the winter melon juice lactic acid bacteria fermentation to a vacuum homogenizer and stirring at 50-100 rpm. The mixed powder is then added, and the mixture is vacuumed to 0.06-0.08 MPa and homogenized at 3000-5000 rpm for 5-10 minutes to form a paste-like consistency. The sterilization process uses… 60 Sterilization by Co-γ irradiation, with an irradiation dose of 5-10 kGy.

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