Anoxic-resistant melon compound beverage and preparation method thereof

Abstract

The application discloses an anti-hypoxia radix glabra composite solid beverage and a preparation method thereof, and belongs to the technical field of health-care beverages. The anti-hypoxia radix glabra composite solid beverage is prepared from radix glabra of nine years as raw material, and comprises the following steps: screening and cleaning, twice soaking, 80 DEG C segmented cooking, 70 DEG C gradient concentration, and low-temperature drying at 65 DEG C or below to obtain high-activity radix glabra extract; then, the radix glabra extract is mixed with white sugar and 95% edible alcohol according to a mass ratio of 1:2:1, and the mixture is dried at 50 DEG C for 2 hours, granulated for 1 hour to obtain the solid granular beverage. The anti-hypoxia radix glabra composite solid beverage has the advantages of maximizing the retention of anti-hypoxia active ingredients, improving the taste and solubility, and the like. Animal experiments prove that the product can significantly relieve the weight loss, pulmonary edema and brain edema caused by hypoxia, regulate key indexes such as HIF-alpha, EPO, TNF-alpha, IL-6 and SOD, and has no obvious organ toxicity and high safety when taken for a long time. The anti-hypoxia radix glabra composite solid beverage is suitable for industrial production, can be used for developing health-care food for resisting hypoxia and relieving the plateau reaction, and provides a standardized scheme for high-value utilization of radix glabra.

Description

Technical Field

[0001] The present invention belongs to the technical field of health care beverages, and particularly relates to a radish beverage for alleviating the body's hypoxia reaction and a preparation method thereof. Background Art

[0002] Radish is a traditional Tibetan medicine plant of the cruciferous family Brassica. It mainly grows in alpine regions above 3,800 meters above sea level and has significant characteristics of being both a medicine and a food. It is rich in bioactive components such as flavonoids, polysaccharides, glucosinolates, alkaloids, organic acids, and various minerals, and also contains essential nutrients such as proteins and vitamins. Research shows that radish has various physiological functions such as anti-hypoxia, anti-oxidation, anti-fatigue, regulating immune homeostasis, improving blood sugar and blood lipids, and alleviating discomfort due to acclimatization. Based on its clear active components, good palatability, and cultivation adaptability, radish has become a functional food raw material with high cost performance and important development prospects, especially suitable for developing health foods targeting the plateau hypoxia environment and related metabolic regulation.

[0003] However, the resource development and utilization of radish are still in the initial stage. In production areas such as Qinghai and Tibet, radish is mostly used as an ordinary crop, with relatively low economic added value. The in-depth research on its natural anti-hypoxia characteristics is still relatively limited. The existing processing methods are mostly direct juicing or powder making by crushing, and the process is relatively simple, failing to fully exert the functional potential of its active components. In addition, the pungent smell contained in radish itself, if directly eaten in powder form, not only affects the taste, but also the bioaccessibility and utilization rate of the active components are relatively low. The currently commercially available radish beverages generally have problems such as poor taste and insufficient optimization of anti-hypoxia and other effects, and fail to effectively achieve the expected effect of alleviating altitude sickness. Therefore, how to improve the taste, quality, and anti-hypoxia function of radish products through process improvement has become an important research direction with scientific significance and market value at present. Summary of the Invention

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides an anti-hypoxia radish beverage and a preparation method thereof.

[0005] In the specific preparation process of the present invention, first, the selected nine-year radish raw materials are cleaned and soaked, and then subjected to segmented steaming and concentration operations in sequence. After extraction and drying steps, radish extracts are obtained. Then, the radish extracts are weighed and mixed with auxiliary materials such as white sugar and alcohol according to a set ratio, and through full mixing, drying, and granulation processes, a granular beverage product is finally obtained. This method maximally retains the activity of each component through the process of separately extracting and then compounding each raw material.

[0006] On the one hand, the present invention provides a radish solid beverage product with anti-hypoxia function. Among them, the preparation method of the core raw material radish extract specifically includes the following industrial production steps:

[0007] S1: Select 500 kg of qualified nine-year-old coriander roots, screen and clean them. The cleaning process lasts for 8 hours.

[0008] S2: Place the cleaned coriander root obtained in S1 into an extraction pot, add purified water and soak for 2 hours, then change the water and soak overnight again;

[0009] S3: The soaked roots obtained in S2 are steamed in an extraction pot at a controlled temperature of 80℃ for 4 hours. S4: Concentrate the cooking liquid obtained in S3, controlling the concentration temperature at 70℃ and the concentration time at 2 hours. S5: Soak the concentrated extract of coriander root obtained in S4 in the extraction pot overnight again; S6: The material obtained after the second soaking in S5 is further cooked in the extraction pot for a second time, with the temperature controlled at 80℃ and the cooking time at 3 hours. S7: The secondary cooking liquor obtained in S6 is subjected to secondary concentration treatment, with the concentration temperature controlled at 70℃ and the concentration time at 3 hours. S8: Collect S7 to obtain concentrated substrate, which is 200 kg of coriander root extract; S9: The coriander root extract obtained in S8 is dried, with the drying temperature controlled to not exceed 65℃ and the drying time to be 72 hours. After drying, approximately 100 kg of dried coriander root extract is obtained.

[0010] To prepare solid beverage granules, the method further includes the following steps: S10: Weigh the ingredients according to the formula, including the dried coriander root extract obtained in step S9, white sugar, and 95% edible alcohol in a ratio of 1:2:1; S11: Put the ingredients from S10 into a mixing device and mix for 5 hours until uniform; S12: Dry the uniformly mixed material obtained in S11 for 2 hours, controlling the drying temperature at 50°C; S13: Granulate the material dried in S12 using a granulator for 1 hour to finally obtain the coriander root solid granule beverage.

[0011] In another aspect, the present invention provides the application of the above-mentioned coriander root solid granule beverage in the preparation of anti-hypoxia products.

[0012] This invention demonstrates a significant anti-hypoxia effect by intervening in a hypoxia-induced mouse model using a coriander root solid granule beverage. Furthermore, this invention verifies the toxicity of the coriander root solid granule beverage through toxicity testing. The body weight, liver function, kidney function, blood lipid levels, and myocardial enzyme levels of mice were measured one and four weeks after consumption of the beverage, and no significant organ toxicity was found. This invention is beneficial for developing the edible and medicinal value of coriander root and broadening its application in anti-hypoxia treatment.

[0013] Specifically:

[0014] A1: The coriander root solid granule beverage prepared by this invention has a significant alleviating effect on weight loss in mice caused by hypoxia;

[0015] A2: The coriander root solid granule beverage prepared by this invention significantly improves hypoxia-induced pulmonary edema and cerebral edema;

[0016] A3: The coriander root solid granule beverage prepared in this invention significantly improves the levels of hypoxia-inducible factor-α (HIF-α), erythropoietin (EPO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and superoxide dismutase (SOD) in hypoxic mice.

[0017] A4: Coix lacryma-jobi solid granule beverage significantly improved the expression of hypoxia-inducible factor-α (HIF-α) in the liver of hypoxic mice.

[0018] A5: Toxicity results of coriander root solid granule beverage showed that after drinking the coriander root solid granule beverage for one and four weeks, there were no significant changes in mouse body weight, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), blood urea nitrogen (BUN), high-density lipoprotein (HDL), low-density lipoprotein (LDL), creatine kinase isoenzyme (CKMB), total bilirubin (TBIL), and direct bilirubin (DBIL).

[0019] The technical effects and advantages of this invention are as follows:

[0020] The solid beverage obtained by this invention can be used to prepare health foods or special dietary foods that have the effects of anti-hypoxia, relieving altitude sickness, improving hypoxic tissue damage, antioxidation, and anti-inflammation.

[0021] Highly effective preservation of active ingredients: Double soaking + segmented cooking + gradient concentration significantly improves the extraction rate and stability of anti-hypoxia components such as flavonoids and polysaccharides;

[0022] Excellent taste and palatability: The compound sugar and alcohol are used to adjust the texture, eliminate the spiciness, and the granules dissolve quickly and are convenient to drink;

[0023] It has a definite anti-hypoxia effect: it significantly relieves weight loss, pulmonary edema and cerebral edema caused by hypoxia, regulates key indicators such as HIF-α, EPO, TNF-α, IL-6 and SOD, and improves the liver's hypoxia response;

[0024] Safe and non-toxic: Acute and subacute toxicity tests showed no significant adverse effects on mouse body weight, liver and kidney function, blood lipids, myocardial enzymes and major organs, and it is safe for long-term use;

[0025] The process is industrializable: the parameters are stable and highly controllable, making it suitable for large-scale production and increasing the added value of coriander root resources. Attached Figure Description

[0026] Figure 1 This is a schematic diagram illustrating the preparation of a coriander root solid granule beverage.

[0027] Figure 2 Indicators of purslane root solid granule beverage in alleviating hypoxia in mice. Figure 2 A is a schematic diagram of the construction of a mouse hypoxia model; Figure 2 B shows the changes in body weight in mice in a hypoxic model after intervention with coriander root solid granule beverage; Figure 2 C is a statistical graph of the dry-wet ratio of the lungs of mice in a hypoxia-relieving model of pyrrolizum root solid granule beverage (detecting pulmonary edema). Figure 2 D is a statistical graph of the dry and wet specific gravity of the brain of mice in the hypoxia-relieving model of coriander root solid granule beverage (detecting cerebral edema). Figure 2 E is a statistical graph showing the expression of hypoxia-inducible factor-α (HIF-α) in the serum of mice in a hypoxia-alleviating model model of coriander root solid granule beverage; Figure 2 F is a statistical graph showing the expression of serum erythropoietin (EPO) in mice with hypoxia-relieving model induced by coriander root solid granule beverage; Figure 2 G is a statistical graph of interleukin-6 (IL-6) expression in mice with hypoxia-relief model by coriander root solid granule beverage; Figure 2 H is a statistical graph showing the expression of tumor necrosis factor-α (TNF-α) in the serum of mice in a hypoxia-relieving model model of coriander root solid granule beverage; Figure 2 Figure I shows the statistical graph of superoxide dismutase (SOD) expression in mice with hypoxia model improved by coriander root solid granule beverage. Figure 2 Image J represents a representative image from immunohistochemical analysis of hepatic hypoxia-inducible factor-α (HIF-α) expression in a hypoxic model mouse model induced by a distillate of coriander root granules. Mouse groups: Control-H2O (normal mice - water group); Control-Brassica (normal mice - distillate of coriander root granules group); Hypoxia-H2O (hypoxic mice - water group); Hypoxia-Brassica (hypoxic mice - distillate of coriander root granules group).

[0028] Figure 3 Results of toxicity test on mice after one week of intervention with coriander root solid granule beverage

[0029] Figure 3 A statistical chart of mouse weight measured during the first week after intervention with a coriander root solid granule beverage; and Figure 3 B serum alanine aminotransferase (ALT). Figure 3 C-aspartate aminotransferase (AST). Figure 3 Creatinine D (CREA) Figure 3 E-urea nitrogen (BUN) Figure 3 F High-density lipoprotein (HDL) Figure 3 G low-density lipoprotein (LDL) Figure 3 H-creatine kinase isoenzyme (CKMB) Figure 3 Total bilirubin (TBIL) Figure 3 J Direct bilirubin (DBIL) level statistics. Figure 3 K represents representative H&E staining results of major mouse organs (heart, liver, spleen, lung, and kidney). Control-H2O (normal mice - drinking water group); Control-Brassica (normal mice - coriander root solid granule beverage group).

[0030] Figure 4 Results of toxicity test on mice after four weeks of intervention with coriander root solid granule beverage

[0031] Figure 4 A statistical chart of mouse weight measured at week 4 after intervention with a coriander root solid granule beverage; and Figure 4 B serum alanine aminotransferase (ALT). Figure 4 C-aspartate aminotransferase (AST). Figure 4 Creatinine D (CREA) Figure 4 E-urea nitrogen (BUN) Figure 4 F High-density lipoprotein (HDL) Figure 4 G low-density lipoprotein (LDL) Figure 4 H-creatine kinase isoenzyme (CKMB) Figure 4 Total bilirubin (TBIL) Figure 4 J Direct bilirubin (DBIL) level statistics. Figure 4 K represents representative H&E staining results of major mouse organs (heart, liver, spleen, lung, and kidney). Control-H2O (normal mice - drinking water group); Control-Brassica (normal mice - coriander root solid granule beverage group). Detailed Implementation

[0032] The application effects of this invention will be described in detail below so that those skilled in the art can more clearly understand the advantages and features of this invention, and thus form a more accurate and clear understanding of the scope of protection of this invention. It should be noted that the following embodiments are only for illustrating this invention and do not constitute any limitation on the scope of protection of this invention.

[0033] Example 1: Preparation of Coriander Root Solid Particle Beverage

[0034] Includes the following steps:

[0035] (1) Select 500 kg of qualified nine-year-old coriander roots, thoroughly screen and wash for 8 hours, then place them in an extraction pot and soak them in water overnight.

[0036] (2) The soaked roots in step (1) were steamed in an extraction pot at 80°C for 4 h, the steaming liquid was collected, and the steaming liquid was concentrated at 70°C for 2 h.

[0037] (3) Place the concentrated extract of coriander root obtained in step (2) back into the extraction pot, add water and soak overnight, then cook for 3 hours at 80°C, and collect the second cooking liquid.

[0038] (4) The secondary cooking liquid obtained in step (3) is concentrated for 3 hours at 70°C. The concentrated substrate is collected to obtain about 200 kg of coriander root extract.

[0039] (5) The root extract obtained in step (4) is dried at a temperature not exceeding 65°C for 72 h to obtain approximately 100 kg of dried root extract for later use.

[0040] (6) Weigh the dried coriander root extract, white sugar and 95% edible alcohol in a mass ratio of 1:2:1, and put the above materials into the mixing equipment and mix them thoroughly for 5 hours until uniform.

[0041] (7) The mixture obtained in step (6) is dried at 50°C for 2 hours, and then granulated by a granulator for 1 hour to finally obtain coriander root solid granule beverage. Specifically, as follows... Figure 1 As shown.

[0042] Example 2: Application of Coriander Root Solid Particle Beverage in Anti-hypoxia

[0043] Specific experimental steps:

[0044] Hypoxia mouse model construction and experimental grouping: Six-week-old male C57BL / 6J mice were selected as experimental subjects and randomly divided into Control-H2O (normal mice - drinking water group); Control-Brassica (normal mice - coriander root solid pellet beverage group); Hypoxia-H2O (hypoxia mice - drinking water group); Hypoxia-Brassica (hypoxia mice - coriander root solid pellet beverage group).

[0045] This invention establishes the following animal grouping and treatment scheme:

[0046] The experimental animals were randomly divided into four groups: (1) Control-H2O group (normal mice-water group): raised under normoxic conditions and allowed free access to ordinary drinking water; (2) Control-Brassica group (normal mice-coriander root solid granule beverage group): raised under normoxic conditions and allowed free access to coriander root solid granule beverage; (3) Hypoxia-H2O group (hypoxic mice-water group): subjected to hypoxia treatment and allowed free access to ordinary drinking water; (4) Hypoxia-Brassica group (hypoxic mice-coriander root solid granule beverage group): 7 days before the implementation of hypoxia treatment, coriander root solid granule beverage was administered under normoxic conditions.

[0047] The dosage of the coriander root solid granule beverage was 0.5 g / mL, administered via free intake.

[0048] From day 8 to day 18 of the experiment, the Hypoxia-H2O and Hypoxia-Brassica groups were subjected to intermittent hypoxia exposure: they were placed in a hypoxic environment with an oxygen concentration of 8.5% for 12 hours daily for 7 consecutive days to establish a hypoxia model. During the hypoxia treatment, the Hypoxia-Brassica group had free access to a beverage containing coriander root granules, while the Hypoxia-H2O group had free access to regular drinking water. The model construction process is illustrated below. Figure 2 As shown in Figure A.

[0049] Throughout the experiment, daily changes in mouse body weight were recorded for each group (see [link to experiment]). Figure 2 B), used to assess general physiological status.

[0050] On day 19 of the experiment, all mice were euthanized. Blood samples were then collected, serum was separated, and relevant biochemical indicators were detected using a fully automated biochemical analyzer. Lung and brain tissues were taken to determine the wet-to-dry weight ratio. Liver tissue was fixed, embedded in paraffin, and sectioned for immunohistochemical staining and hematoxylin-eosin staining analysis.

[0051] The steps for collecting mouse lung and brain tissue and determining their wet-dry specific gravity are as follows:

[0052] (1) Collect mouse lung and brain tissue, wash the blood on the tissue surface with pre-cooled phosphate buffer solution, wipe the tissue surface dry with filter paper, and quickly weigh the wet weight in place and record the data.

[0053] (2) Place the tissue in a constant temperature drying oven and dry at 60 degrees Celsius for 12 hours until constant weight. After removal, weigh the dry weight and record the data. Calculate the tissue wet-to-dry ratio and moisture content according to the formulas: wet-to-dry ratio = wet weight / dry weight; moisture content (%) = (wet weight − dry weight) / wet weight × 100%. Specific results can be found in [the table below]. Figure 2 C and Figure 2 D. Hypoxia-H2O (hypoxic mice - drinking water group) showed obvious pulmonary edema and cerebral edema, while the edema in Hypoxia-Brassica (hypoxic mice - coriander root solid granule beverage group) was significantly relieved.

[0054] The steps for detecting the expression of hypoxia-inducible factor-α (HIF-α), erythropoietin (EPO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and superoxide dismutase (SOD) in mouse serum by ELISA are as follows:

[0055] (1) Sample processing: After whole blood collection, place at room temperature for 2 hours, centrifuge at 1000×g for 20 minutes, and carefully collect the supernatant serum. Remove the ELISA kit from the refrigerator and allow it to equilibrate at room temperature for 20-30 minutes.

[0056] (2) Standard dilution: Dissolve the lyophilized standard and dilute it multiple times.

[0057] (3) Add 100 μL of each diluted standard to the standard wells. Add 50 μL of the mouse serum to be tested to the sample wells. Seal the reaction wells with sealing film and incubate at 37°C for 120 minutes.

[0058] (4) Washing the plate: Discard the liquid in the well. Fill each well with washing solution, soak for 1-2 minutes, shake dry and pat clean. Repeat 3 times.

[0059] (5) Add biotinylated antibody: Add 100 μL of biotinylated detection antibody working solution to each well. Incubate at 37℃ for 60 minutes. Repeat the washing step 3 times.

[0060] (6) Add enzyme conjugate: Add 100 μL of horseradish peroxidase (HRP)-labeled avidin working solution to each well. Incubate at 37°C in the dark for 30-45 minutes. Repeat the washing step 5 times.

[0061] (7) Color development: Add 90 μL of TMB substrate to each well. Incubate at 37℃ in the dark for 15-30 minutes. Termination and reading: Add 50 μL of stop solution to each well. The blue color will immediately turn yellow. Within 5 minutes of adding the stop solution, measure the OD value of each well using a microplate reader at a wavelength of 450 nm. The results are as follows: Figure 2 E, Figure 2 F, Figure 2 G, Figure 2 H and Figure 2The results showed that in the Hypoxia-H2O (hypoxic mouse-water group) mice, serum hypoxia indices—hypoxia-inducible factor-α (HIF-α) and erythropoietin (EPO), as well as inflammatory indices—tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)—were significantly elevated, while the antioxidant indices—superoxide dismutase (SOD)—were significantly decreased. In the Hypoxia-Brassica (hypoxic mouse-coriander root solid granule beverage group) mice, the hypoxia condition was significantly alleviated.

[0062] The steps for histopathological staining are as follows:

[0063] (1) Tissue dehydration and embedding: Lung tissue was fixed in 4% paraformaldehyde for 24 hours and then dehydrated. After dehydration, it was embedded.

[0064] (2) Paraffin sectioning and drying: Preheat the tissue microtome to a suitable temperature, carefully install the blades, and group and label the slides. Freeze the paraffin at -20℃. After freezing, trim the paraffin block with a blade, leaving 1-2 mm around the lung area. Place the trimmed paraffin block on the microtome, first trimming it to a thickness of 20 μm until the lung outline is complete, then adjusting to a thickness of 5 μm for continuous sectioning. Gently place the slide into the microtome using tweezers and a brush, spreading the slide completely flat, and carefully transfer it to a glass slide. After sectioning, bake in an oven at 60℃ for 1 hour, then stain or use as needed.

[0065] (3) Dewaxing and water removal: Soak the dried tissue sections in xylene I, II and III in a fume hood for 20 minutes each, then put them into anhydrous ethanol I and II for 15 minutes each, then into 95% anhydrous ethanol I and II for 5 minutes each, then into 80% anhydrous ethanol for 10 minutes, and finally rinse slowly with running water for 3-5 minutes.

[0066] (4) Immunohistochemical staining steps

[0067] 1) The paraffin sections were placed in xylene and ethanol in sequence to complete dewaxing and gradient hydration. Finally, the sections were transferred to distilled water for later use.

[0068] 2) Place the slide in citrate buffer and heat to repair the antigen. After cooling to room temperature, rinse thoroughly with PBS.

[0069] 3) Immerse the slides in a 3% hydrogen peroxide solution to block endogenous peroxidase activity. Then rinse the slides with PBS to remove excess reagent.

[0070] 4) Add 10% goat serum to the surface of the slide and block at room temperature for 30 minutes.

[0071] 5) Add primary antibody working solution, including hypoxia-inducible factor-α (HIF-α) antibody, to the surface of the slide. Incubate overnight at 4°C to ensure adequate binding.

[0072] 6) The next day, bring the sections to room temperature and wash with PBS, then add the secondary antibody working solution. Incubate at room temperature for 30 minutes to achieve specific binding.

[0073] 7) Add DAB chromogenic solution to the slide and observe under a microscope. A brownish-yellow deposit indicates a positive signal. Once the color has developed to an appropriate depth, immediately rinse with distilled water to terminate the reaction.

[0074] 8) Counterstain the cell nuclei with hematoxylin, and rinse with running water after staining for 30 seconds.

[0075] 9) The sections were dehydrated with ethanol and then cleared with xylene. Finally, they were mounted with neutral resin and dried for observation. Results are as follows: Figure 2 J showed that the liver tissue of the Hypoxia-H2O (hypoxic mouse-water group) group showed significant high expression of HIF-1a, while the expression of HIF-1a in the Hypoxia-Brassica (hypoxic mouse-coriander root solid granule beverage group) group was significantly alleviated.

[0076] (5) Hematoxylin-eosin staining procedure

[0077] 1) After dewaxing and removing the tissue sections from the water, immerse them in hematoxylin staining solution for 5–10 minutes (adjust the specific time according to the type of staining solution and the results of the preliminary experiment). After immersion, rinse with tap water for 3–5 minutes.

[0078] 2) Place the slice in 1% hydrochloric acid alcohol for rapid differentiation for a few seconds (about 3–10 seconds). Under a microscope, observe that the cell nuclei are clear and appear blue-purple. Then immediately rinse with running water to stop differentiation.

[0079] 3) Place the slices in an alkaline blueing solution for 30 seconds to 1 minute to turn the cell nuclei from purplish-red to blue, then rinse with running water for 3 to 5 minutes.

[0080] 4) Immerse the slides in eosin staining solution for 1–3 minutes, then remove them and quickly and gently wash them with distilled water or proceed directly to the dehydration step without washing.

[0081] 5) Dehydrate the slices by immersing them in 80% ethanol, 95% ethanol I and II, and anhydrous ethanol I and II for 2–5 minutes each.

[0082] 6) Place the sections in xylene I and II sequentially for 5–10 minutes each to make the tissue transparent.

[0083] 7) Add a drop of neutral resin mounting medium, carefully cover with a coverslip to avoid air bubbles, and let it air dry at room temperature.

[0084] 8) After the mounting medium has completely dried, observe the tissue morphology and acquire images under an optical microscope. See below for specific results. Figure 3 k and Figure 4 There were no significant differences in tissue staining between control-H2O (normal mice - drinking water group) and control-Brassica (normal mice - coriander root solid granule beverage group), demonstrating that the coriander root solid granule beverage had no significant toxic effects on mouse organs.

[0085] The fully automated detection steps for biochemical indicators in mouse serum are as follows:

[0086] (1) Collect whole blood from mice, let it stand at room temperature for 2 h, and then centrifuge at 3000 r / min for 15 min at 2–8 ℃ to obtain serum.

[0087] (2) Carefully aspirate the upper layer of serum and dispense it into centrifuge tubes.

[0088] (3) The aliquoted serum samples were placed on a fully automated biochemical analyzer for testing. See below for specific results. Figure 3 B- Figure 3 J and Figure 4 B- Figure 4 There were no significant differences in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), blood urea nitrogen (BUN), high-density lipoprotein (HDL), low-density lipoprotein (LDL), creatine kinase isoenzyme (CKMB), total bilirubin (TBIL), and direct bilirubin (DBIL) levels between Control-H2O (normal mice - drinking water group) and Control-Brassica (normal mice - coriander root solid granule beverage group), demonstrating that the coriander root solid granule beverage had no significant organ toxicity in mice.

[0089] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A method for preparing a hypoxia-resistant coriander root compound solid beverage, characterized in that, The process includes two stages: preparation of coriander root extract and solid particle forming. The specific steps are as follows: (1) Raw material pretreatment: Nine-year-old coriander roots were selected, screened, and washed with running water for 8 hours; (2) Soaking once: Soak the cleaned coriander roots in purified water for 2 hours, change the water and soak overnight again; (3) First cooking and concentration: The soaked roots of the coriander were cooked at 80°C for 4 hours, the cooking liquid was collected and concentrated at 70°C for 2 hours to obtain a first concentrated liquid; (4) Second soaking: Add water to the concentrated solution from the first soaking and soak overnight; (5) Secondary cooking and concentration: The material after the second soaking is cooked at 80°C for 3 hours, the secondary cooking liquid is collected and concentrated at 70°C for 3 hours to obtain the root extract; (6) Low-temperature drying: The root extract of coriander is dried at a temperature not exceeding 65°C for 72 hours to obtain the dried product of coriander root extract; (7) Ingredient compounding: Weigh and mix the ingredients according to the mass ratio of dried coriander root extract: white sugar: 95% edible alcohol = 1:2:1; (8) Mixing, drying and granulation: Mix the ingredients for 5 hours until uniform, dry at 50°C for 2 hours, and granulate for 1 hour to obtain the hypoxia-resistant coriander root compound solid beverage.

2. The preparation method according to claim 1, characterized in that, The cooking in steps (3) and (5) is carried out in a sealed extraction pot, and the concentration is carried out under reduced pressure.

3. A hypoxia-resistant coriander root compound solid beverage, characterized in that, The beverage is prepared by the method described in any one of claims 1-3; the beverage uses nine-year-old coriander root extract as the core functional ingredient, and the excipients are white sugar and 95% edible alcohol, with a mass ratio of 1:2:1; the coriander root extract is prepared by double soaking, segmented cooking, gradient concentration and low-temperature drying.

4. The hypoxia-resistant coriander root compound solid beverage according to claim 3, characterized in that, The beverage can significantly alleviate hypoxia-induced weight loss, pulmonary edema, and cerebral edema, downregulate the levels of hypoxia-inducible factor HIF-α, erythropoietin EPO, tumor necrosis factor TNF-α, and interleukin-6 IL-6, and increase superoxide dismutase (SOD) activity, thereby improving the expression of hypoxia-inducible factor-α (HIF-α) in hypoxic mice.

5. The hypoxia-resistant coriander root compound solid beverage according to claim 3, characterized in that, The beverage, when taken continuously for 1 week or 4 weeks, had no significant effect on mouse body weight, liver and kidney function indicators ALT, AST, CREA, BUN, blood lipid indicators HDL, LDL, myocardial enzyme indicators CKMB, bilirubin indicators TBIL, DBIL, or the morphology of heart, liver, spleen, lung, and kidney tissues, and showed no obvious acute or subacute organ toxicity.

6. The application of the anti-hypoxia coriander root compound solid beverage according to any one of claims 3-5 in the preparation of health foods or special dietary foods that are anti-hypoxic, relieve altitude sickness, improve hypoxic tissue damage, have antioxidant and anti-inflammatory properties.

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