A skin repair composition, its preparation and use

Abstract

The application belongs to the field of skin care products, and particularly relates to a skin repair composition as well as a preparation method and application thereof. The skin repair composition comprises dragon blood extract, grape seed extract and an antioxidant. The mass fractions of the dragon blood extract and the grape seed extract are 5-15 parts and 5-15 parts respectively. The antioxidant comprises at least two of hydrolyzed procyanidin, dimethyl methoxy benzodihydro pyranol and ferulic acid. Compared with the prior art, the skin repair composition has the advantages of simple formula, small irritation, excellent repair and soothing effect, good stability and the like.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, and in particular to a skin repair composition, its preparation method, and its application. Background Technology

[0002] As the largest organ in the human body, the skin serves as an immune barrier to prevent microbial invasion and maintain internal homeostasis. It also plays many important roles, including thermoregulation, fluid balance, and wound healing. When the skin is damaged by endogenous factors (such as aging and disease) or exogenous factors (such as physical trauma, ultraviolet radiation, and chemical damage), it initiates a series of complex biological mechanisms to actively restore its structural integrity, barrier function, and appearance. This process includes wound repair and barrier function restoration.

[0003] However, when skin damage extends deep into the dermis (such as cuts, soft tissue tears, surgical incisions, etc.) or involves large areas of epidermal loss (such as abrasions, burns, etc.) or damage to the body's own fibroblasts (such as stretch marks), resulting in severe damage to the soft tissue that cannot fully repair itself and is instead replaced by fibrous tissue for repair, scars will form, thus affecting the skin's appearance. To avoid or eliminate scars, many people choose repair cosmetics to repair damaged skin.

[0004] Currently, repair cosmetics often use ingredients such as ceramides, panthenol, and asiaticoside, but these ingredients have many limitations in terms of repair effects:

[0005] The skin repair effect of single ceramides is limited after reaching a certain concentration. Moreover, due to their lipophilic nature, they have poor solubility in aqueous environments, making them difficult to apply directly to water-based skin care products, resulting in low bioavailability.

[0006] Panthenol has poor stability. For example, a skincare product containing 5% panthenol will only have about 20% panthenol residue after about 8 months at room temperature. Furthermore, while increasing the panthenol content in skincare products can improve their effectiveness, higher concentrations result in a thicker, more viscous product, significantly impacting the user experience. Additionally, using panthenol alone cannot achieve rapid redness reduction in a short period.

[0007] Asiaticoside is the main active ingredient in Centella asiatica, which can significantly promote wound healing and inhibit scar formation. In cosmetics, it has anti-inflammatory, sedative, detoxifying, and anti-swelling effects. However, due to its large molecular weight, it has poor water and lipid solubility, making it difficult to cross the skin barrier when applied topically, resulting in low absorption and bioavailability.

[0008] In addition to the problems with the ingredients themselves, existing repair cosmetics also have the following shortcomings: Limited depth of repair: Most repair products mainly work on the outermost layer of the skin, the stratum corneum, to repair the cortex by replenishing lipids and moisturizing. However, they are almost unable to effectively repair deep damage to the dermis caused by inflammation, aging, and photodamage, such as collagen degradation and elastic fiber breakage.

[0009] The products are highly homogenized and offer limited efficacy: Ingredient lists across different brands are extremely similar, lacking truly groundbreaking solutions. These products often only offer some relief for a specific problem, failing to address multiple concurrent issues such as oxidative damage and inflammatory responses. For existing skin damage, especially long-standing conditions like stretch marks, the repair effect is not significant.

[0010] The formulation has flaws: In pursuit of "all-around effectiveness," product formulations often pile on a large number of active ingredients, crudely layering multiple highly active components. This may lead to poor compatibility or counteracting effects, and could also alter the pH and permeability of the formulation, resulting in synergistic stimulation. Furthermore, many highly effective repair ingredients (such as certain peptides, natural antioxidants, and growth factors) are extremely unstable and easily deactivated during production and storage. The process of formulation engineers seeking stable solutions may reduce their bioavailability. In addition, many repair raw materials are expensive, resulting in high prices for finished products, limiting the consumer base and hindering product promotion.

[0011] Therefore, there is an urgent need to develop a new skin repair composition that is simple in formulation, highly stable, has good skin repair effect, and is less irritating. Summary of the Invention

[0012] Based on this, the purpose of the present invention is to overcome the defects or deficiencies of the prior art and provide a skin repair composition that is simple in formulation, highly stable, has good skin repair effect, and is less irritating.

[0013] A skin repair composition comprising dragon's blood extract, grape seed extract, and an antioxidant; wherein the dragon's blood extract and grape seed extract are present in parts by weight of 5-15 parts and 5-15 parts, respectively; and the antioxidant comprises at least two of hydrolyzed proanthocyanidins, dimethylmethoxybenzodihydropyranol, and ferulic acid.

[0014] Compared to existing technologies, the skin repair composition of this invention utilizes different components along different repair pathways. Dragon's blood extract accelerates wound repair by promoting fibroblast proliferation and collagen synthesis; plant extracts such as grape seed extract provide antioxidant and anti-inflammatory support, inhibiting the release of inflammatory factors (TNF-α, IL-6, IL-1β) and reducing oxidative stress damage. The antioxidants synergistically work with dragon's blood extract and plant extracts to form a "sacrifice-regeneration" cycle, preventing the active ingredients in dragon's blood extract and plant extracts from becoming ineffective, thereby improving the overall stability of the composition. Furthermore, this skin repair composition has advantages such as simple formulation and low irritation, making it suitable for preparing skin repair products.

[0015] In one embodiment, the method for preparing the dragon's blood extract includes: pulverizing the resin secreted by Peruvian croton, soaking the resin in water at a material-to-liquid ratio of 1:10 to 1:15 to allow the resin to fully swell, then extracting at 90°C to 100°C for 1.5 to 2 hours, repeating the extraction 2 to 3 times, combining all the extracts and concentrating them to 10% to 15% of the original volume to obtain the dragon's blood extract.

[0016] In one embodiment, the method for preparing the grape seed extract includes: pulverizing dried grape seeds, adding grape juice to 70% ethanol at a material-to-liquid ratio of 1:5 to 1:10, extracting at 50°C to 70°C for 1 to 3 hours, repeating the extraction 1 to 3 times, combining all extracts, concentrating, and freeze-drying to obtain the grape seed extract.

[0017] In one embodiment, the antioxidant is hydrolyzed proanthocyanidins and dimethylmethoxybenzodihydropyranol, with mass fractions of 0.05~0.2 parts and 0.01~0.2 parts, respectively.

[0018] In one embodiment, the skin repair composition further includes dipropylene glycol, 1,3-propanediol, and water, in parts by weight of 15-25 parts, 50-60 parts, and 1-5 parts, respectively.

[0019] In addition, the present invention also provides a repair essence, which is made by mixing phase A, phase B, phase C and phase D; Phase A includes water, EDTA-2Na, sodium polyacrylate, and betaine; Phase B includes preservatives and 1,3-butanediol; Phase C includes sodium hyaluronate and the skin repair composition; Phase D includes preservatives and glycerol glucosides.

[0020] In one embodiment, by mass parts: Phase A comprises 82-88 parts water, 0.01-0.05 parts EDTA-2Na, 0.01-0.2 parts sodium polyacrylate, and 1-2 parts betaine; Phase B includes 0.1 to 0.4 parts of preservative and 1 to 3 parts of 1,3-butanediol; Phase C includes 0.1 to 0.3 parts sodium hyaluronate and 5 to 15 parts skin repair composition; Phase D includes 0.4 to 0.8 parts of preservative and 0.5 to 1.5 parts of glycerol glucoside.

[0021] This invention also provides a method for preparing the aforementioned repair essence, characterized by comprising the following steps in sequence: S1. Heat phase A to 80℃~85℃ and stir to dissolve; heat phase B to 50℃~55℃ and stir to dissolve. S2. After phase A cools down to 50°C, add phase B to phase A and stir to dissolve. S3. After the mixture from step S2 has cooled to 42°C, add phase C to the mixture from step S2 and stir to dissolve. S4. Add the dissolved D phase to the mixture from step S3 and stir until homogeneous.

[0022] The present invention also provides the use of the skin repair composition in the preparation of skin repair products or stretch mark repair products.

[0023] In one embodiment, the skin repair product includes toner, skin gel, skin lotion, skin cream, serum, gel, mask, and spray.

[0024] To better understand and implement this invention, the following detailed description is provided in conjunction with the accompanying drawings. Attached Figure Description

[0025] Figure 1 The free radical scavenging rate of compositions 1-9 is given.

[0026] Figure 2 The DPPH free radical scavenging rate of compositions 1-9.

[0027] Figure 3 The content of inflammatory factors in compositions 1-9.

[0028] Figure 4 The TNF-α downregulation rate of compositions 1-9.

[0029] Figure 5 The IL-6 downregulation rate of compositions 1-9.

[0030] Figure 6 The IL-1β downregulation rate of compositions 1-9.

[0031] Figure 7 The ABTS radical scavenging rate of composition 10-14 is given.

[0032] Figure 8 The DPPH free radical scavenging rate of composition 10-14.

[0033] Figure 9 The free radical scavenging rate is denoted as 1-4.

[0034] Figure 10 Hyaluronidase inhibition rate of the skin repair compositions of Examples 1 and Comparative Examples 1-3.

[0035] Figure 11 The skin heme is the skin repair composition of Example 1 and Comparative Examples 1-3.

[0036] Figure 12 The difference in skin hemoglobin between the skin repair compositions of Example 1 and Comparative Examples 1-3 is shown.

[0037] Figure 13 The percentage of the red area in the skin repair compositions of Examples 1 and Comparative Examples 1-3.

[0038] Figure 14 The difference in the percentage of red area between the skin repair compositions of Example 1 and Comparative Examples 1-3.

[0039] Figure 15 Example 1 shows the reduction of skin redness area using the repair essence of Example 1.

[0040] Figure 16 Example 2 shows the reduction of skin redness area using the repair essence from Example 1.

[0041] Figure 17 The images show a before-and-after comparison of stretch marks before and after using the repair essence in Example 1.

[0042] Figure 18 Comparison of stretch marks before and after using the microneedle sponge in the control group. Detailed Implementation

[0043] Skin wound repair is a dynamic, complex, and continuous biological process, mainly involving a series of events such as hemostasis, inflammatory response, tissue proliferation (granulation tissue formation), and tissue remodeling (scar formation). Growth factors are a class of bioactive polypeptides secreted by cells that regulate cellular function, primarily participating in wound repair and tissue regeneration. EGF (Epidermal Growth Factor) acts extensively on epithelial cells, fibroblasts, keratinocytes, and various stem cells, stimulating cell division and proliferation, promoting cell migration, and accelerating wound healing and tissue repair. TGF (Transforming Growth Factor) affects almost all cell types, exhibiting a bidirectional regulatory effect; it can both promote cell proliferation and inhibit excessive growth, while also stimulating the synthesis of extracellular matrix proteins such as collagen. Increased inflammatory responses in the body can lead to tissue damage and impair wound healing.

[0044] The skin barrier function primarily resides in the stratum corneum of the epidermis. This "brick-and-mortar structure" (keratinocytes + intercellular lipids) is a crucial barrier preventing the invasion of harmful external factors and the loss of nutrients and moisture from the body. When the sebum film (a natural moisturizing film) and intercellular lipids (such as ceramides and cholesterol) are insufficient, the skin's ability to retain moisture decreases, making it easier for external irritants (bacteria, pollutants, ultraviolet rays, etc.) to penetrate. This leads to increased sensory nerve transmission signals, causing sensitive reactions such as redness, stinging, dryness, and sensitivity. Skin barrier repair refers to restoring the integrity and function of the outermost "brick-and-mortar structure" (physical barrier), mainly the epidermis, especially the stratum corneum. This is achieved by accelerating the metabolism, differentiation, and shedding of keratinocytes and promoting the synthesis and assembly of intercellular lipids (ceramides, cholesterol, fatty acids), thereby restoring and maintaining the skin's moisturizing ability, resistance to external stimuli, and microecological balance.

[0045] The relationship between skin wound repair and barrier repair is not independent, but rather mutually influential and inseparable. Skin wound repair includes barrier repair, specifically manifested in the later stages of wound repair (the proliferation and remodeling phase), where the newly formed epidermis needs to complete the final differentiation of barrier function to form an effective stratum corneum. Therefore, one of the ultimate goals of wound repair is to rebuild a fully functional barrier.

[0046] Therefore, the primary objective of this invention is to screen for repair components that promote growth factor secretion and accelerate wound healing as the main active ingredients in skin repair compositions. Due to the advantages of plant extracts, such as natural origin, high safety, multi-target repair, comprehensive efficacy, and low irritation, this invention screens for target repair components from plant extracts. Based on the characteristic that fibroblasts can secrete insulin-like growth factor, platelet-derived growth factor, TGF-β1 (transforming growth factor-β1), and EGF, this invention applies a combination of plant extracts with excipients / solvents (water, dipropylene glycol, and 1,3-propanediol) to fibroblasts and investigates its effect on growth factor secretion.

[0047] Screening revealed that dragon's blood extract is rich in alkaloids (taspine), which can promote the secretion of TGF-β1 and EGF. TGF-β1 and EGF can stimulate the proliferation of keratinocytes and fibroblasts, inhibit the activity of metalloproteinases, and facilitate collagen deposition, thus promoting wound healing. Specifically, taspine accelerates wound repair and tissue regeneration by promoting the proliferation and migration of fibroblasts and vascular endothelial cells, stimulating collagen and extracellular matrix synthesis. Taspine can also inhibit pain signal transmission and reduce pain perception by activating opioid receptors in the central nervous system, while indirectly alleviating inflammatory pain through anti-inflammatory effects (such as inhibiting the NF-κB pathway). Furthermore, dragon's blood extract is rich in polyphenols such as proanthocyanidins. Proanthocyanidins protect cell membranes and DNA from oxidative damage by scavenging free radicals (such as reactive oxygen species, ROS), inhibiting lipid peroxidation, and inhibiting the release of inflammatory mediators (such as the NF-κB signaling pathway). Proanthocyanidins and taspinine work synergistically to enhance the antioxidant and anti-inflammatory effects of dragon's blood extract.

[0048] Among them, dragon's blood extract refers to extracts from the genus *Rhizoma* of the palm family (Arecaceae). Daemonorops ), Fabaceae family, Pterocarpus genus ( Pterocarpus ), Agavaceae family, Dracaena genus ( Dracaena ), Euphorbiaceae family, Croton genus ( Croton The resins and juices were extracted from over 20 species of plants belonging to four different families and genera. The content of active ingredients in dragon's blood extracts varies depending on the source and extraction method. Therefore, this invention optimizes the source and extraction method of dragon's blood extract to increase the content of proanthocyanidins and taspine. Specifically, the dragon's blood extract of this invention is derived from *Croton burmannii*, a plant belonging to the genus *Croton* of the family Euphorbiaceae. Croton lechleriThe resin secreted by the agent is extracted using the following method: The lumpy resin is crushed and passed through a 20-40 mesh sieve to obtain a uniform powder. Then, the raw material and pure water are added to an extraction tank at a material-to-liquid ratio of 1:10 to 1:15 (g / mL), and soaked at room temperature for 1-2 hours to allow the material to fully swell. The extraction stage uses a decoction method, heating to 90℃-100℃ and maintaining a gentle boil. Each extraction lasts 1.5-2 hours, and this process is repeated 2-3 times to fully extract the water-soluble components. After extraction, the extracts are combined while still hot (>60℃) and sequentially passed through a 100-mesh sieve and a plate and frame filter press for solid-liquid separation to obtain a clear filtrate. The obtained filtrate is then further removed by centrifugation or fine filtration to remove insoluble impurities. The preliminarily purified filtrate was then transferred to a concentration device for vacuum concentration until the soluble solids content (measured by a refractometer, °Brix) reached 20%~30% (equivalent to 10%~15% of the original filtrate volume), in order to recover some water and obtain a high-concentration dragon's blood extract solution. Analysis showed that the active ingredients of the dragon's blood extract of this invention include 40%~70% polyphenols (mainly proanthocyanidins), 10%~20% diterpenoids, 5%~15% lignans, and 0.5%~2.5% alkaloids (mainly taspine). Taspine is specific and has extremely strong biological activity, and is considered the most important single active ingredient of the dragon's blood extract and a key indicator for quality control. Proanthocyanidins are the largest active ingredient in the dragon's blood extract, forming the basis of its efficacy.

[0049] Furthermore, this invention optimized the concentration of dragon's blood extract in the composition, finding that the composition was more effective when the mass percentage of dragon's blood extract was between 5% and 20%. Excessive concentration of taspine in the dragon's blood extract leads to overexpression of TGF-β1, which may conversely cause scarring and fibrosis, limiting the efficacy of the composition and making it difficult to improve its efficacy by increasing the concentration of dragon's blood extract.

[0050] Therefore, this invention considers combining dragon's blood extract with other plant extracts to increase the content of other active ingredients while controlling the concentration of taspine, and to improve the repair effect of the composition through the synergistic effect of different components.

[0051] Oxidative free radicals and inflammatory factors can damage the skin barrier. Therefore, this invention aims to improve the composition's ability to repair the skin barrier by enhancing its antioxidant and anti-inflammatory capabilities, thereby improving the composition's repair effect by simultaneously repairing skin wounds and the skin barrier.

[0052] This invention combines plant extracts with excipients / solvents, and then measures the antioxidant and anti-inflammatory capabilities of the compositions. Four plant extracts with good effects were screened out: grape seed extract, ginkgo leaf extract, lotus seed extract, and tea extract. These four plant extracts were then compounded with dragon's blood extract, and water, dipropylene glycol, and 1,3-propanediol were added as excipients and solvents to form four compound combinations. The mass ratios of the components in each compound combination are shown in Table 1. Subsequently, by testing the antioxidant and anti-inflammatory capabilities of each combination, the component of the combination with the best effect was selected as the active ingredient of the skin repair composition.

[0053] Table 1. Composition of Candidate Skin Repair Compositions (by weight percentage)

[0054] The preparation method of grape seed extract is as follows.

[0055] First, dried grape seeds were crushed to a particle size of 1-3 mm and extracted with 70% (v / v) ethanol solution at a material-to-liquid ratio of 1:8 at 60°C with stirring for 120 minutes. The extraction was repeated once. The extracts were combined and concentrated under vacuum at 50°C until no alcohol odor remained. Purification is the crucial step in obtaining a high-purity product: the aqueous concentrate was pumped into an ADS-17 or HP-20 macroporous resin column at a flow rate of 1.5 BV / h, followed by thorough washing with 4 BV-5 BV of deionized water to remove impurities such as sugars. Then, an ethanol gradient elution was performed, first washing away some impurities with a low concentration of ethanol (20%-30%), followed by main elution with 60%-70% ethanol. The target fraction rich in proanthocyanidins was precisely collected using a UV detector (280 nm). After being concentrated under vacuum at 50°C, the fraction was freeze-dried under vacuum (pre-freezing at -45°C, main drying stage ≤ -20°C, vacuum degree 0.05 mBar) to produce a fluffy powder in order to retain maximum activity. The final product was standardized by spectrophotometry to ensure that the proanthocyanidin (calculated as catechin equivalent) content reached 95%.

[0056] The preparation method of lotus seed extract is as follows.

[0057] First, the cored and dried lotus seeds were pulverized and passed through a 30-mesh sieve. During the extraction stage, a 50% (v / v) ethanol aqueous solution was added at a material-to-liquid ratio of 1:12 (w / v), and ultrasonic-assisted extraction was employed (power 400W, frequency 40kHz, temperature 55±5℃). After extraction for 40 minutes, solid-liquid separation was performed, and the residue was extracted once more. The extracts were combined and concentrated under vacuum at 55℃~60℃ to completely recover the ethanol, yielding a concentrated aqueous solution. To further enrich the active ingredients, the concentrated solution was passed through a D101 macroporous resin column at a flow rate of 1.5 BV / h. It was first eluted with deionized water, then eluted with 60% ethanol solution, and the polyphenol-rich fraction was collected. Finally, the eluent was spray-dried (inlet air 170℃, outlet air 80℃), and 10%~20% maltodextrin was added as a carrier to obtain a free-flowing light brown powder. The final product was standardized using the Folin-Ciocalteu method, controlling the total polyphenol content to be no less than 30% or 50%.

[0058] The preparation method of tea extract is as follows.

[0059] First, dried green tea leaves were pulverized and passed through a 20-mesh sieve. Then, at a material-to-liquid ratio of 1:10 (w / v), a 60% (v / v) ethanol aqueous solution was used for extraction at 70±2℃ with constant temperature stirring for 90 minutes. This extraction was repeated once, and the extracts were combined. The extract was then concentrated under vacuum at 40℃~50℃ to approximately 1 / 5 of its original volume, and then refrigerated at 4℃ for 12 hours to allow impurities to precipitate. After centrifugation and fine filtration, a crude extract was obtained. The key purification step employed AB-8 macroporous adsorption resin: after loading the crude extract at a flow rate of 2 BV / h, water-soluble impurities were washed away with 3 BV~5 BV of deionized water, followed by elution of the target tea polyphenols with a 70% ethanol solution at a flow rate of 2 BV / h. The target fraction was collected under UV detection (280 nm). Finally, the eluent was concentrated under vacuum at 50℃ and then powdered by spray drying (inlet air 180℃, outlet air 85℃) or vacuum freeze-drying. The finished product undergoes standardized processing to ensure that the total polyphenol content is ≥90% or the content of the key active ingredient epigallocatechin gallate (EGCG) is ≥50%.

[0060] The preparation method of Ginkgo biloba extract is as follows.

[0061] Ginkgo leaves were pulverized and passed through a 10-20 mesh sieve, then dynamically extracted with a 60% (w / w) acetone aqueous solution at a 1:10 material-to-liquid ratio (w / v) at 55-60°C for approximately 4 hours using countercurrent extraction. The extract was first vacuum distilled at ≤60°C to recover the acetone. A crucial detoxification and purification step followed: the concentrate was allowed to settle at room temperature and centrifuged. The resulting clear liquid was then subjected to liquid-liquid extraction with equal volumes of petroleum ether and ethyl acetate, retaining the aqueous phase rich in the target components. This aqueous phase was further purified using an HPD macroporous adsorption resin column, washing successively with water and low-concentration ethanol, followed by elution with 60-70% ethanol to remove the target components. The eluent was concentrated and dried, then analyzed using high-performance liquid chromatography (HPLC) and other sophisticated instruments to ensure that the active ingredients met the standards and that toxic ginkgolic acid was completely removed, ultimately yielding a standardized light yellow-brown extract powder.

[0062] In summary, combining the above four plant extracts with dragon's blood extract, if their antioxidant and anti-inflammatory effects are significantly enhanced, could potentially serve as a candidate active ingredient for a skin repair composition that can achieve repair effects through a multidimensional mechanism.

[0063] Then, by testing the antioxidant and anti-inflammatory properties of combinations 1 to 9, the present invention screens out the components of the combination with the best compounding effect as the active ingredients of the skin repair composition.

[0064] This invention uses the Total Antioxidant Capacity (T-AOC) Assay Kit (ABTS Method) (Product No.: D799298-0100) from Shanghai Sangon Biotech Co., Ltd. to determine the total antioxidant capacity of the skin repair composition.

[0065] The ABTS method is the most widely used indirect detection method, applicable to the determination of the antioxidant capacity of both hydrophilic and lipophilic substances. ABTS, upon oxidation, generates stable blue-green cationic free radicals ABTS+, which are soluble in aqueous or acidic ethanol media and exhibit maximum absorption at 734 nm. When the analyte is added to an ABTS+ solution, its antioxidant components react with the ABTS+, causing the reaction system to decolorize. The change in absorbance at 734 nm is detected, and the free radical scavenging rate of the sample is calculated.

[0066] like Figure 1As shown, the scavenging rates of ABTS free radicals by dragon's blood extract, grape seed extract, lotus seed extract, tea extract, and ginkgo leaf extract (i.e., combinations 1-5) were 48.11%, 53.72%, 32.96%, 52.74%, and 41.84%, respectively. The scavenging rates of ABTS free radicals by the (dragon's blood + grape seed) combination, (dragon's blood + lotus seed) combination, (dragon's blood + tea) combination, and (dragon's blood + ginkgo leaf) combination (i.e., combinations 6-9) were 71.08%, 46.61%, 70.3%, and 50.85%, respectively. Among them, the (dragon's blood + grape seed) combination exhibited the highest free radical scavenging rate, significantly higher than the other three combinations and the individual dragon's blood extract, grape seed extract, lotus seed extract, tea extract, and ginkgo leaf extract (P<0.05).

[0067] This invention uses the DPPH free radical scavenging ability test kit (product number: D799008-0100) from Shanghai Sangon Biotech Co., Ltd. to determine the scavenging rate of the skin repair composition against DPPH free radicals.

[0068] DPPH radicals are stable nitrogen-centered free radicals and are an important indicator of a sample's antioxidant capacity, widely used in research on antioxidant foods, health products, and pharmaceuticals. DPPH radicals have unpaired electrons, their alcoholic solutions are purple, and they exhibit strong absorption at 515 nm. In the presence of antioxidants, DPPH radicals are scavenged, the solution color lightens, and the absorbance at 515 nm decreases. Within a certain range, the change in absorbance is directly proportional to the degree of radical scavenging.

[0069] like Figure 2 As shown, the scavenging rates of DPPH free radicals by dragon's blood extract, grape seed extract, lotus seed extract, tea extract, and ginkgo leaf extract (i.e., combinations 1-5) were 63.57%, 58.03%, 39.75%, 61.00%, and 46.53%, respectively. The scavenging rates of ABTS free radicals by the (dragon's blood + grape seed) combination, (dragon's blood + lotus seed) combination, (dragon's blood + tea) combination, and (dragon's blood + ginkgo leaf) combination (i.e., combinations 6-9) were 75.77%, 57.09%, 73.79%, and 56.28%, respectively. Among them, the (dragon's blood + grape seed) combination exhibited the highest free radical scavenging rate, significantly higher than the other three combinations and the individual dragon's blood extract, grape seed extract, lotus seed extract, tea extract, and ginkgo leaf extract (P<0.05).

[0070] This invention uses the following method to test the anti-inflammatory properties of combinations 1-9: RAW264.7 macrophages from 96-well plates with well-developed monolayers were selected. The culture medium in the 96-well plates was discarded, and the samples were diluted to a non-cytotoxic concentration for testing. LPS and culture medium containing combinations 1-9 (i.e., dragon's blood extract, grape seed extract, lotus seed extract, tea extract, ginkgo leaf extract, (dragon's blood + grape seed) combination, or (dragon's blood + lotus seed) combination, (dragon's blood + tea) combination, or (dragon's blood + ginkgo leaf) combination) were added to each well of the sample group. Cell culture medium containing LPS was added to each well of the model control group. Culture medium containing dexamethasone sodium phosphate and LPS was added to each well of the positive control group. Cell culture medium was added to each well of the blank control group. 100 μL of culture medium was added to each well, with 6 wells in each group. After sample addition, the 96-well plates were incubated at 37°C in a 5% CO2 incubator for (24±2) h. After incubation, the supernatant was collected and the relative levels of three inflammatory factors [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β)] were measured. The levels of TNF-α, IL-6, and IL-1β were measured according to the ELISA kit instructions and the downregulation rate was calculated. Downregulation rate = (1 - T / C) × 100%; where T refers to the average level of inflammatory factors in the sample group and C refers to the average level of inflammatory factors in the model control group.

[0071] like Figure 3 As shown, after LPS induction, the levels of inflammatory factors in the RAW264.7 strain were significantly higher than those in the blank control group (P<0.05), confirming the validity of the experimental model. Among the samples, the (dragon's blood + grape seed) combination showed the lowest levels of IL-6 and IL-1β, at 98.49 pg / mL and 85.38 pg / mL, respectively.

[0072] Figure 4 The study showed that the downregulation rate of the inflammatory factor TNF-α content by each sample was significantly higher than that of other single substances and combinations. Figure 5 The study showed the downregulation rate of IL-6 content for each sample. The downregulation rates of tea extract and the (dragon's blood + grape seed) combination were 44.59% and 49.64%, respectively, significantly higher than other single substances and combinations (p<0.05). The downregulation rates of dragon's blood extract and grape seed extract alone were 30.09% and 32.59%, respectively, while the downregulation rate of the (dragon's blood + grape seed) combination was significantly higher, demonstrating that the combination of the two has a synergistic effect and can enhance the anti-inflammatory effect. Figure 6The results showed the downregulation rate of IL-1β content for each sample. The downregulation rates of dragon's blood extract and grape seed extract were 49.62% and 51.45%, respectively, while the downregulation rate of the (dragon's blood + grape seed) combination was 57.61%, which was significantly higher than that of the single treatment and other combinations (P<0.05), consistent with the results for IL-6.

[0073] TNF-α, IL-6, and IL-1β are key pro-inflammatory cytokines that play a central role in skin inflammation, sensitivity, aging, and barrier damage. TNF-α participates in photoaging, its expression increases after UV exposure, leading to collagen degradation, and it is a key mediator of skin aging. IL-6 rapidly increases after injury or infection, and its overexpression hinders the recovery of normal barrier function. IL-1β is one of the core signals of barrier damage, associated with stinging and burning sensations in the skin. Both inflammatory factors, IL-6 and IL-1β, are closely related to skin wound repair and barrier repair. When used alone, dragon's blood extract and grape seed extract showed moderate downregulation rates for both, while the combination of dragon's blood and grape seed extract significantly increased their downregulation rates and enhanced their inhibitory effects, indicating that the combination of dragon's blood extract and grape seed extract has a better effect on skin wound repair and barrier repair. In contrast, tea extract is more effective than grape seed extract when used alone, but its effect when combined with dragon's blood extract is slightly inferior to that of dragon's blood extract and grape seed extract alone. Furthermore, grape seeds, as a processing byproduct of wine, grape juice, and other products, have advantages such as wide availability, low price, and mature production technology. Therefore, considering the anti-inflammatory and antioxidant capabilities of the composition, as well as the availability and cost of plant extracts, this invention further investigated the combination of dragon's blood extract and grape seed extract.

[0074] To determine the concentration and ratio of dragon's blood extract and grape seed extract, this invention combines dragon's blood extract and grape seed extract at different concentrations, as shown in Table 2. The final concentration and ratio of the two are determined by evaluating the free radical scavenging rate of each combination.

[0075] Table 2 Component Ratio Optimization Settings

[0076] Figures 7-8The scavenging rates of ABTS and DPPH free radicals by combinations 10-14 were shown in the figure. As can be seen from the figure, the scavenging rate increased with increasing the concentration of either dragon's blood extract or grape seed extract, indicating that increasing the concentration enhances their effect. When both dragon's blood extract and grape seed extract were increased to 10%, the free radical scavenging rate continued to increase. However, when the concentrations of both dragon's blood extract and grape seed extract were increased to 20%, the increase in free radical scavenging rate was very limited and showed no statistical difference, indicating that further increasing the concentration had little effect on the synergistic effect of the combination. Therefore, this invention sets the concentration of both dragon's blood extract and grape seed extract at 10%.

[0077] However, after the antioxidant capacity of the combination of dragon's blood extract and grape seed extract was significantly improved, a new problem emerged: the composition exhibited poor stability and a significant decrease in activity after long-term storage. Research revealed that the active ingredients in the extracts are highly susceptible to auto-oxidation and photo-oxidation. For example, the phenolic hydroxyl groups in active ingredients such as proanthocyanidins readily undergo dehydrogenation reactions with free radicals, generating semiquinone free radicals. If the concentration of free radicals in the environment is too high, the semiquinone free radicals may further react, leading to the destruction of the active ingredient's own structure and loss of antioxidant capacity. During auto-oxidation, the oxidation of one active molecule may generate new free radicals, triggering the oxidation of more molecules and resulting in "self-accelerated" degradation. Furthermore, the conjugated double bonds and aromatic ring structures in active ingredients such as proanthocyanidins are photosensitive, especially under ultraviolet (UV) irradiation. Light energy can excite molecular electronic transitions, producing photo-oxidation products (such as quinone compounds), leading to the deactivation of the active ingredients. During photo-oxidation, light energy may directly decompose the active ingredients, generating new free radicals (such as singlet oxygen), which further attack other molecules, accelerating the overall oxidation process.

[0078] To maintain the antioxidant capacity of the extract and improve the stability of the composition, this invention considers combining plant extracts with other antioxidants to enhance the overall stability of the composition through a "sacrifice-regeneration" cycle. To further improve the stability of the composition, this invention selects appropriate antioxidants for both auto-oxidation and photo-oxidation pathways, providing a comprehensive antioxidant barrier for the composition.

[0079] Therefore, this invention screens antioxidants targeting singlet oxygen, the core active substance of photo-oxidation, and peroxide radicals, a key intermediate in auto-oxidation. It was found that dimethylmethoxybenzodihydropyranol can act simultaneously on both auto-oxidation and photo-oxidation pathways, excelling at quenching singlet oxygen and scavenging peroxide radicals. However, while using dimethylmethoxybenzodihydropyranol alone improves the stability of the composition to some extent, it is still limited. Studies have shown that dimethylmethoxybenzodihydropyranol has relatively weak scavenging power against hydroxyl radicals, which are strong oxidants capable of attacking almost all biomolecules and can be generated through photochemical oxidation, metal-catalyzed decomposition, and auto-oxidation side reactions. Therefore, this invention also selects an antioxidant with strong hydroxyl radical scavenging effects, which can complement dimethylmethoxybenzodihydropyranol, and the two are combined to form a complete and highly efficient antioxidant defense system.

[0080] The composition of each sample after the addition of antioxidants is shown in Table 3. The samples were subjected to free radical scavenging test and stability test (standing at 25℃ for 90 days).

[0081] Table 3 Samples with different combinations of antioxidants

[0082] Table 4. Stability test results for each sample

[0083] Figure 9 The scavenging rates of each sample against ABTS and DPPH free radicals were shown. After the addition of antioxidants, the scavenging rates of the samples against free radicals were improved to varying degrees, with sample 3 showing the highest scavenging rates against both ABTS and DPPH free radicals.

[0084] Table 4 shows the stability changes of each sample after the addition of antioxidants. As can be seen from Table 4, sample 3 performed excellently in the stability test and no abnormalities were found, indicating that dimethylmethoxybenzodihydropyranol and hydrolyzed proanthocyanidins contribute to the stability of the composition.

[0085] Therefore, the composition and proportion of the skin repair composition of the present invention are determined as shown in Table 5.

[0086] Table 5. Components and Proportions of Skin Repair Compositions

[0087] Furthermore, the present invention also conducted stability tests on the skin repair composition in Table 5, setting four conditions: -10℃, 25℃, alternating -10℃ / 25℃, and 48℃. No abnormalities were observed on days 0, 30, and 90, indicating that the skin repair composition is very stable.

[0088] Based on the above skin repair composition, the present invention provides a repair essence comprising: phase A, phase B, phase C, and phase D, and in volume parts as follows: Phase A consists of 82-88 parts deionized water, 0.01-0.05 parts EDTA-2Na, 0.01-0.2 parts Covacryl AC (sodium polyacrylate), and 1-2 parts betaine.

[0089] Phase B comprises 0.1 to 0.4 parts of p-hydroxyacetophenone and 1 to 3 parts of 1,3-butanediol.

[0090] Phase C includes 0.1 to 0.3 parts sodium hyaluronate and 5 to 15 parts skin repair composition.

[0091] Phase D consists of 0.4–0.8 parts of p-hydroxyacetophenone and 0.5–1.5 parts of glycerol glucoside.

[0092] The preparation method of the above-mentioned repair essence includes the following steps.

[0093] S1. Heat phase A to 80℃~85℃ and stir to dissolve. Heat phase B to 50℃~55℃ and stir to dissolve.

[0094] S2. After phase A cools down to 50°C, add phase B to phase A and stir to dissolve.

[0095] S3. After the mixture from step S2 has cooled to 42°C, add phase C to the mixture from step S2 and stir to dissolve.

[0096] S4. Add the dissolved D phase and stir until well mixed.

[0097] The skin repair composition of the present invention will be described in detail below with reference to specific embodiments and accompanying drawings.

[0098] Example 1 This embodiment provides a skin repair composition, the components and contents of which are shown in Table 5.

[0099] Based on the above skin repair composition, this embodiment also provides a repair essence, the formula of which is shown in Table 6.

[0100] Table 6. Ingredients and Content of Repair Essence

[0101] The preparation method of the repair essence includes the following steps.

[0102] S1. Heat phase A to 80℃~85℃ and stir to dissolve. Heat phase B to 50℃~55℃ and stir to dissolve.

[0103] S2. After phase A cools down to 50°C, add phase B to phase A and stir to dissolve.

[0104] S3. After the mixture from step S2 has cooled to 42°C, add phase C to the mixture from step S2 and stir to dissolve.

[0105] S4. Add the dissolved D phase and stir until well mixed.

[0106] The above-mentioned skin repair composition was subjected to stability tests under four storage conditions: -10℃, 25℃, alternating -10℃ / 25℃, and 48℃, for a duration of 90 days. The results showed that the skin repair composition did not separate or precipitate after 90 days of storage, demonstrating good stability.

[0107] Comparative Examples 1-3 Comparative Examples 1-3 provide a repair essence, the preparation method and ingredients of which are basically the same as the repair essence of Example 1, the difference being the skin repair composition. The components and contents of the skin repair compositions used in Comparative Examples 1-3 are shown in Table 7.

[0108] Table 7. Components and content of skin repair compositions in Comparative Examples 1-3

[0109] Test Example 1 The inhibition rate of hyaluronidase activity by the skin repair compositions of Examples 1 and Comparative Examples 1-3 was determined using the hyaluronidase activity assay kit (540nm spectrophotometry) from Beijing Tianjingsha Gene Technology Co., Ltd.

[0110] Hyaluronidase specifically hydrolyzes hyaluronic acid, generating oligosaccharide fragments containing reducing terminals (such as N-acetylglucosamine). Under alkaline conditions, these reducing sugars react with 3,5-dinitrosalicylic acid reagent to produce a brownish-red aminonitrosalicylic acid. The color intensity is directly proportional to the reducing sugar content, and a maximum absorption peak is observed at 540 nm. The catalytic activity of hyaluronidase can be quantified by measuring the OD540 value corresponding to the reducing sugars produced in the enzymatic reaction. In inhibition experiments, the inhibition rate of hyaluronidase activity can be calculated by comparing the change in OD540 value of the reaction system before and after sample addition.

[0111] In evaluating the soothing efficacy of cosmetics, the hyaluronidase inhibition assay is a very important and classic in vitro evaluation method. Figure 10The results showed that the negative control inhibited hyaluronidase activity by 3.15%, while the positive control (1 mg / mL dimethyl glycyrrhizate) inhibited hyaluronidase activity by 79.78%. Compared with the negative control, the skin repair compositions of Example 1, Comparative Examples 1 and 2 significantly increased the inhibition rate of hyaluronidase. The skin repair composition of Example 1 showed an inhibition rate of 32.94% on hyaluronidase, significantly higher than that of Comparative Examples 1, 2, and 3 (p<0.05). These experimental results demonstrate that the skin repair composition of the present invention can significantly inhibit hyaluronidase activity, protect hyaluronic acid in the skin from decomposition, thereby maintaining the skin barrier, relieving sensitivity, and achieving a "soothing" effect.

[0112] Test Example 2 120 healthy men or women aged 18 to 60 were selected; subjects with lactic acid stinging score ≥3, who felt sensitive skin and were prone to redness were selected. The testing method referred to "T / CAB 0152-2022 Test Method for Seven Efficacy Items of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing and Soothing".

[0113] 50 μL of a 0.05% capsaicin solution was dropped onto two layers of filter paper (0.8 cm thick) and placed on both cheeks of the subject. After capsaicin stimulation, the left cheek was treated with the repair essence of Example 1 or Comparative Examples 1-3, while the right cheek was treated with a matrix control sample (i.e., a blank control) without the repair agent. Skin parameters were measured at various time points: T0 – before application of the repair essence; T1 – immediately after capsaicin stimulation; T30 min – 30 min after application of the repair essence; T60 min – 60 min after application of the repair essence; T90 min – 90 min after application of the repair essence.

[0114] Data were analyzed using SPSS 26.0 statistical software. Independent t-tests or rank-sum tests were used to compare parameter values ​​and differences at each follow-up time point in the sample experimental area and the blank control area between groups. Paired t-tests or rank-sum tests were used to compare parameter values ​​at each follow-up time point within the sample experimental area itself.

[0115] (1) Skin hemoglobin Depend on Figure 11 It can be seen that after capsaicin stimulation modeling, the skin hemoglobin values ​​T1 in the blank control and the four repair essence areas were significantly different from the baseline value (T0) immediately (p<0.05), indicating that capsaicin stimulation modeling was successful.

[0116] Depend on Figure 12It can be seen that, compared with the value immediately after modeling (T1), the skin hemoglobin in the blank control decreased by 6.26%, 12.51%, and 20.13% at T30 min, T60 min, and T90 min, respectively; the skin hemoglobin in the skin repair composition area decreased by 11.83%, 22.69%, and 34.85% at T30 min, T60 min, and T90 min, respectively; the skin hemoglobin in Comparative Example 1 decreased by 5.67%, 9.72%, and 19.92% at T30 min, T60 min, and T90 min, respectively; the skin hemoglobin in Comparative Example 2 decreased by 15.74%, 21.49%, and 23.5% at T30 min, T60 min, and T90 min, respectively; and the skin hemoglobin in Comparative Example 3 decreased by 9.16%, 13.75%, and 23.43% at T30 min, T60 min, and T90 min, respectively. At T30 min, T60 min, and T90 min, the difference in skin hemoglobin in the skin repair composition was significantly greater than that in the blank control, and the degree of decrease in skin hemoglobin was greater than that in the blank control, comparative example 1, comparative example 2, and comparative example 3.

[0117] (2) Percentage of red zone area Depend on Figure 13 It can be seen that after capsaicin stimulation modeling, the percentage of red area in the blank control and the four repair essence areas (T1) were significantly different from the baseline value (T0) (p<0.05), indicating that capsaicin stimulation modeling was successful.

[0118] Depend on Figure 14 It was found that, compared with the value immediately after modeling (T1), the proportion of skin red area in the blank control area decreased by 25.81% and 45.44% at T60 min and T90 min, respectively; the proportion of skin red area in the skin repair composition area decreased by 29.86% and 66.87% at T60 min and T90 min, respectively; the proportion of skin red area in Comparative Example 1 decreased by 22.64% and 47.76% at T60 min and T90 min, respectively; the proportion of skin red area in Comparative Example 2 decreased by 30.22% and 55.69% at T60 min and T90 min, respectively; and the proportion of skin red area in Comparative Example 3 decreased by 27.25% and 52.32% at T60 min and T90 min, respectively. Compared with the value immediately after modeling (T1), the difference in the proportion of skin red area in the skin repair composition area was significantly greater than that in the blank control, Comparative Example 1, Comparative Example 2, and Comparative Example 3 (p < 0.05).

[0119] Test Example 3 First, a microneedle sponge was used on the stretch mark area to open the skin channels, allowing for better absorption of the repair essence. Then, the repair essence from Example 1 was used continuously for 7 days. The blank control group used only the microneedle sponge but not the repair essence.

[0120] Depend on Figure 17 and Figure 18 As can be seen, compared with the blank control group, after continuous use of the repair essence for 7 days following the microneedling treatment, the vertical stretch marks below the navel were significantly reduced, and the skin in the entire stretch mark area became smoother, although itching and other discomforts were also observed during this period. Since the repair essence does not cause discomfort on undamaged skin, and the discomfort from the microneedling treatment disappears after 1-2 days, this indicates that the discomfort caused by the repair essence on damaged skin is a discomfort experienced during skin healing. This proves that the repair essence of this invention can promote the healing of damaged skin, repair the skin barrier, and improve the skin elasticity and texture in the stretch mark area.

[0121] In summary, the skin repair composition and repair essence provided by this invention have the effects of maintaining the skin barrier, relieving sensitivity, and repairing skin damage, including stretch marks. Compared with existing repair products, they have the following advantages: (1) Multi-effect combined for comprehensive repair: Through the synergistic effect of dragon's blood extract (promoting fibroblast proliferation and collagen synthesis) and grape seed extract (antioxidant and anti-inflammatory), it simultaneously repairs dermal damage and the "brick wall structure" of the epidermis. At the same time, the composition achieves multi-pathway skin protection by inhibiting inflammatory factors (TNF-α, IL-6, IL-1β), scavenging free radicals (significantly improving the scavenging rate of ABTS and DPPH free radicals), and inhibiting hyaluronidase activity.

[0122] (2) Simple ingredients and stable formula: It contains only 4 active ingredients (dragon's blood extract, grape seed extract, hydrolyzed proanthocyanidins, and dimethylmethoxybenzodihydropyranol), avoiding the incompatibilities caused by the accumulation of ingredients in traditional products. By adding dimethylmethoxybenzodihydropyranol (quenching singlet oxygen) and hydrolyzed proanthocyanidins (chelating metal ions and inhibiting lipid peroxidation), a dual antioxidant barrier is constructed. There is no precipitation or discoloration after standing at 25℃ for 90 days, and it remains stable under extreme conditions from -10℃ to 48℃.

[0123] (3) The combination of dragon's blood extract and grape seed extract can achieve enhanced core repair and anti-inflammatory effects. Dragon's blood extract can promote wound repair and inhibit the release of inflammatory factors; grape seed extract can resist oxidation and inflammation. When the two are combined, the free radical scavenging rate is significantly improved, the downregulation rate of inflammatory factors is increased, and the anti-inflammatory effect is enhanced.

[0124] (4) The combination of hydrolyzed proanthocyanidins and dimethylmethoxybenzodihydropyranol can enhance antioxidant defense. Hydrolyzed proanthocyanidins have a strong ability to scavenge free radicals and can also protect other active ingredients; dimethylmethoxybenzodihydropyranol can quench singlet oxygen and stabilize the formula. When the two work synergistically, the free radical scavenging rate is improved and the stability is significantly better than the control group.

[0125] (5) The combination of dipropylene glycol and 1,3-propanediol can achieve a synergistic effect of solvent and moisturizing. Dipropylene glycol can enhance the penetration of active ingredients, while 1,3-propanediol has a solvent effect and provides basic moisturizing. The combination of the two can optimize the skin feel and avoid stickiness.

[0126] (6) Controllable cost and wide applicability: Grape seed extract and dragon's blood extract have abundant raw material sources and mature extraction processes. This skin repair composition can be widely used in various cosmetics such as ointments, creams, lotions, and liquids to meet the needs of different consumer scenarios.

[0127] The embodiments described above are merely examples of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A skin repair composition, characterized in that, It includes dragon's blood extract, grape seed extract, and antioxidants; the dragon's blood extract and grape seed extract are in parts by weight of 5-15 parts and 5-15 parts, respectively; the antioxidants include at least two of hydrolyzed proanthocyanidins, dimethylmethoxybenzodihydropyranol, and ferulic acid.

2. The skin repair composition according to claim 1, characterized in that, The preparation method of the dragon's blood extract includes: pulverizing the resin secreted by Peruvian croton, soaking the resin in water at a material-to-liquid ratio of 1:10 to 1:15 to fully swell the resin, then extracting it at 90℃ to 100℃ for 1.5 to 2 hours, repeating the extraction 2 to 3 times, combining all the extracts and concentrating them to 10% to 15% of the original volume to obtain the dragon's blood extract.

3. The skin repair composition according to claim 2, characterized in that, The method for preparing the grape seed extract includes: crushing dried grape seeds, adding grape juice to 70% ethanol at a material-to-liquid ratio of 1:5 to 1:10, extracting at 50℃ to 70℃ for 1 to 3 hours, repeating the extraction 1 to 3 times, combining all extracts, concentrating, and freeze-drying to obtain the grape seed extract.

4. The skin repair composition according to claim 3, characterized in that, The antioxidants are hydrolyzed proanthocyanidins and dimethylmethoxybenzodihydropyranol, with mass fractions of 0.05~0.2 parts and 0.01~0.2 parts, respectively.

5. The skin repair composition according to any one of claims 1 to 4, characterized in that, It also includes dipropylene glycol, 1,3-propanediol and water, in parts by mass of 15-25 parts, 50-60 parts and 1-5 parts, respectively.

6. A repairing essence, characterized in that, It is made from a mixture of phases A, B, C, and D; Phase A includes water, EDTA-2Na, sodium polyacrylate, and betaine; Phase B includes preservatives and 1,3-butanediol; Phase C includes sodium hyaluronate and the skin repair composition according to any one of claims 1 to 5; Phase D includes preservatives and glycerol glucosides.

7. The repair essence according to claim 6, characterized in that, By weight parts: Phase A comprises 82-88 parts water, 0.01-0.05 parts EDTA-2Na, 0.01-0.2 parts sodium polyacrylate, and 1-2 parts betaine; Phase B includes 0.1 to 0.4 parts of preservative and 1 to 3 parts of 1,3-butanediol; Phase C includes 0.1 to 0.3 parts sodium hyaluronate and 5 to 15 parts skin repair composition; Phase D includes 0.4 to 0.8 parts of preservative and 0.5 to 1.5 parts of glycerol glucoside.

8. The method for preparing the repair essence according to claim 6 or 7, characterized in that: The steps are as follows: S1. Heat phase A to 80℃~85℃ and stir to dissolve; heat phase B to 50℃~55℃ and stir to dissolve. S2. After phase A cools down to 50°C, add phase B to phase A and stir to dissolve. S3. After the mixture from step S2 has cooled to 42°C, add phase C to the mixture from step S2 and stir to dissolve. S4. Add the dissolved D phase to the mixture from step S3 and stir until homogeneous.

9. The use of the skin repair composition according to any one of claims 1 to 5 in the preparation of skin repair products or stretch mark repair products.

10. The application according to claim 9, characterized in that, The skin repair products include toners, skin gels, lotions, creams, serums, gels, masks, and sprays.

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